CN102318574B - Method for improving greenling fertilized egg hatching rate - Google Patents
Method for improving greenling fertilized egg hatching rate Download PDFInfo
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- CN102318574B CN102318574B CN2011102393853A CN201110239385A CN102318574B CN 102318574 B CN102318574 B CN 102318574B CN 2011102393853 A CN2011102393853 A CN 2011102393853A CN 201110239385 A CN201110239385 A CN 201110239385A CN 102318574 B CN102318574 B CN 102318574B
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- 241001417941 Hexagrammidae Species 0.000 title claims abstract description 34
- 230000012447 hatching Effects 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 230000000638 stimulation Effects 0.000 claims abstract description 14
- 239000013535 sea water Substances 0.000 claims abstract description 12
- 230000003203 everyday effect Effects 0.000 claims abstract description 10
- 102000002322 Egg Proteins Human genes 0.000 claims description 40
- 108010000912 Egg Proteins Proteins 0.000 claims description 40
- 210000004681 ovum Anatomy 0.000 claims description 40
- 238000011534 incubation Methods 0.000 claims description 19
- 230000002706 hydrostatic effect Effects 0.000 claims description 6
- 230000003760 hair shine Effects 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 235000013601 eggs Nutrition 0.000 abstract description 57
- 239000012528 membrane Substances 0.000 abstract description 9
- 238000005507 spraying Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 2
- 230000003252 repetitive effect Effects 0.000 abstract 1
- 241000251468 Actinopterygii Species 0.000 description 6
- 230000004720 fertilization Effects 0.000 description 6
- 230000013011 mating Effects 0.000 description 5
- 230000009027 insemination Effects 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000009991 scouring Methods 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 241001249573 Hexagrammos Species 0.000 description 1
- 241001596950 Larimichthys crocea Species 0.000 description 1
- 241001529596 Pontinus kuhlii Species 0.000 description 1
- 241000212346 Spermolepis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a method for improving greenling fertilized egg hatching rate, which can solve the problems that the membranes of greenling fertilized eggs are thicker and the hatching rate of the fertilized eggs is low. The technical scheme provided by the invention is that the method comprises the artificial hatching steps of: when sunlight is available, taking fertilized egg merogones out of a hatching pond and exposing the fertilized egg merogones in air to be irradiated by sunlight every day, conducting seawater flow spraying stimulation to the fertilized egg merogones for one time per 10 to 15 minutes of irradiation, keeping the water flow spraying stimulation time to be 30 to 60 seconds per time, keeping the irradiation time to be 1 to 1.5 hours per day and then putting the fertilized egg merogones back into the hatching pond for continuous hatching. By adopting the method provided by the invention, under the repetitive alternate effects of sunlight irradiation and water flow stimulation, the membranes of the fertilized eggs are expanded and shrunk, become fragile and can be broken easily, and therefore the hatching rate of the fertilized eggs is obviously improved. According to contrast tests, compared with the existing hatching method, the method provided by the invention can improve the greenling fertilized egg hatching rate by 3 to 5 percent.
Description
Technical field
The present invention relates to fish fertilization and hatching m, specifically, relate to a kind of method that improves greenling fertilized egg hatching rate.
Background technology
Greenling (
Hexagrammos otakiiJ﹠amp; S) mainly be distributed in China Shandong, Liaoning Coast, be commonly called as yellow croaker, yellow ear of maize, be under the jurisdiction of Rockfish shape order, Greenling section, for settling down fish in the littoral shallow sea of cold warm nature.Because of delicious flavour, economic worth is high, quite is subjected to the coastal culturist of northern China and consumers welcomed.Greenling parent population fish brood amount is low, and fish-egg is the high viscosity fish-egg, ovum footpath 1.8～2.2 mm, easily extruding caking.Present stage, the artificial insemination of fish viscid egg, the rare practice of hatching technique and deliver, limited greatly the development of high viscosity ovum type fish artificial breeding industrialization, the fish-egg fertility rate and hatchability is relatively low under the greenling artificial condition, fertilization rate is about 48% under the prior art condition, and incubation rate only is about 56%.
The applicant has applied for application for a patent for invention, name is called " Greenling artificial insemination and hatching m ", the artificial insemination step of the method is: ripe fish-egg is collected put into the clean plane container of smooth surface, when treating that the ovum amount reaches 100～200g, gently fish-egg is pressed into a plane with have gentle hands, planar thickness is 2～3 layers of ovum, notices that the hand dynamics is light, avoids crushing fish-egg.From the method, can find out, fish-egg is 2～3 layers of ovum, has certain thickness, because fish-egg viscosity is high, the fish-egg the time of fertilization essence ovum contact rate of internal layer is little, thereby causes fertilization rate still lower, simultaneously because the pieces of an egg gathering, inside can't obtain sufficient dissolved oxygen, causes incubation rate also lower, has still restricted the success of greenling artificial breeding.
The problem that exists in order to solve said method, the applicant has applied for application for a patent for invention, " artificial insemination of a kind of high viscosity fish-egg and hatching m ", the main feature of the method is, adopt manual method the greenling ovum to be shaped as the mode of being fertilized again behind the ovum sheet of individual layer fish-egg, avoid fish-egg to become block group, the fish-egg the time of fertilization can fully be contacted with sperm, improved the fish-egg fertilization rate.
But because Greenling fertilized egg egg membrane is thicker and than flexible, be difficult for rupture of membranes, existing artificial incubation method does not have effective measures to this, cause the incubation rate of fertilized egg still on the low side.
Summary of the invention
The invention provides a kind of method that improves greenling fertilized egg hatching rate, it is thicker that it can solve Greenling fertilized egg egg membrane, the problem that the incubation rate of fertilized egg is on the low side.
In order to solve the problems of the technologies described above, technical scheme of the present invention is, a kind of method that improves greenling fertilized egg hatching rate, and the artificial incubation step of described method is:
(1) incubation net cage of fertilized egg ovum sheet being put into the hatchery is hatched, and adopts the mode of hydrostatic, continuous charge and 16 ℃ of water temperature Constant temperature hatchs;
(2) having in the every day of sunshine, fertilized egg ovum sheet is taken out from the hatchery and is exposed in the air, accepting shining of sunshine shines, every photograph solarization was carried out seawater current to fertilized egg ovum sheet in 10～15 minutes and is sprayed stimulation, it is 30～60 seconds that each current spray stimulation time, be 1～1.5 hour according to the solarization time every day, then puts back to and continue hatching in the hatchery;
(3) brooding time of fertilized egg ovum sheet in 16 ℃ of hatcheries is 18～20 days, newly hatched larvae focuses mostly in net cage edge and bottom, then active and move about in water body at the middle and upper levels gradually, when waiting to reach certain density, in time prelarva is shifted out the hatchery and cultivate to cultivating the pond.
Carry out according to shining the slightly weak time period of selection of time sunshine.
According to shine selection of time every year by the end of October～by the end of November the morning 8:30～10:00 time in.
According to shine selection of time every year by the end of October～by the end of November every afternoon 14:30～16:00 time in.
Described fertilized egg ovum sheet is individual layer.
Described fertilized egg ovum sheet is 2～3 layers.
In the mating season of greenling, greenling parent population fecund ovum is on the algae such as the flat river hedge in offshore 1～2m depth of water cay district, Song Zao, and minority originates on the reef.Because tidal action, high water time greenling fish-egg can be submerged in the seawater, and the time of low water fish-egg just is exposed in the air, and is subject to the washing away of irradiation, seawater of the sun, because shrivelled phenomenon can appear in Exposure to Sunlight and drying function fish-egg.Studies show that through the applicant, the exposure of this regularity, sunshine, seawater scouring stimulate, not only do not damage fish-egg, are conducive on the contrary the hatching of fertilized egg.
Inspire thus, high tide under the abundant simulating natural environment of the present invention, greenling fertilized egg is submerged in the seawater, is exposed to airborne rule at ebb tide, be subject to simultaneously the irradiation, seawater scouring of the sun, the situation such as air-dry, in greenling incubating oosperm process, take out fertilized egg and be exposed in the air every day from the hatchery, accept simultaneously to put back to hatchery continuation hatching after the irradiation of sunshine and the current of spraying water stimulate 1～1.5h.In this course, the egg membrane of fertilized egg stimulates repeatedly alternating action through sunshine, air-dry and current, and egg membrane generation breathing changes, and egg membrane becomes fragile, and is easy to rupture of membranes, has obviously improved the incubation rate of fertilized egg.Find through comparative trial, the present invention can improve greenling hatchability of fertile eggs 3～5% than existing hatching m.The present invention has advantages of that operating process is simple, convenient, efficient, for the artificial propagation in scale of greenling is laid a good foundation.
Embodiment
Embodiment 1
Greenling mating season every year by the end of October～by the end of November, the artificial incubation step is:
(1) incubation net cage of individual layer fertilized egg ovum sheet being put into the hatchery is hatched, and adopts the mode of hydrostatic, continuous charge and 16 ℃ of water temperature Constant temperature hatchs;
(2) the every morning 8:30～10:00 of sunshine is being arranged, individual layer fertilized egg ovum sheet is taken out from the hatchery and is exposed in the air, accepting shining of sunshine shines, every photograph solarization was carried out seawater current to fertilized egg ovum sheet in 10 minutes and is sprayed stimulation, it is 30 seconds that each current spray stimulation time, be 1 hour according to the solarization time every day, then individual layer fertilized egg ovum sheet put back to and continued hatching in the hatchery;
(3) brooding time of individual layer fertilized egg ovum sheet in 16 ℃ of hatcheries is 18～20 days, newly hatched larvae focuses mostly in net cage edge and bottom, then active and move about in water body at the middle and upper levels gradually, when waiting to reach certain density, in time prelarva is shifted out the hatchery and cultivate to cultivating the pond.
Embodiment 2
Greenling mating season every year by the end of October～by the end of November, the artificial incubation step is:
(1) incubation net cage of individual layer fertilized egg ovum sheet being put into the hatchery is hatched, and adopts the mode of hydrostatic, continuous charge and 16 ℃ of water temperature Constant temperature hatchs;
(2) 14:30～16:00 every afternoon of sunshine is being arranged, individual layer fertilized egg ovum sheet is taken out from the hatchery and is exposed in the air, accept stimulating according to shining of sunshine, every photograph solarization stimulation was carried out seawater current to fertilized egg ovum sheet in 15 minutes and is sprayed, each current spraying time is 60 seconds, be 1.5 hour according to the solarization time every day, then individual layer fertilized egg ovum sheet put back to and continued hatching in the hatchery;
(3) brooding time of individual layer fertilized egg ovum sheet in 16 ℃ of hatcheries is 18～20 days, newly hatched larvae focuses mostly in net cage edge and bottom, then active and move about in water body at the middle and upper levels gradually, when waiting to reach certain density, in time prelarva is shifted out the hatchery and cultivate to cultivating the pond.
Embodiment 3
Greenling mating season every year by the end of October～by the end of November, the artificial incubation step is:
(1) incubation net cage of individual layer fertilized egg ovum sheet being put into the hatchery is hatched, and adopts the mode of hydrostatic, continuous charge and 16 ℃ of water temperature Constant temperature hatchs;
(2) the every morning 8:30～10:00 of sunshine is being arranged, individual layer fertilized egg ovum sheet is taken out from the hatchery and is exposed in the air, accepting shining of sunshine shines, every photograph solarization was carried out seawater current to fertilized egg ovum sheet in 13 minutes and is sprayed stimulation, it is 45 seconds that each current spray stimulation time, be 1.25 hour according to the solarization time every day, then individual layer fertilized egg ovum sheet put back to and continued hatching in the hatchery;
(3) brooding time of individual layer fertilized egg ovum sheet in 16 ℃ of hatcheries is 18～20 days, newly hatched larvae focuses mostly in net cage edge and bottom, then active and move about in water body at the middle and upper levels gradually, when waiting to reach certain density, in time prelarva is shifted out the hatchery and cultivate to cultivating the pond.
Embodiment 4
Greenling mating season every year by the end of October～by the end of November, the artificial incubation step is:
(1) incubation net cage of 2 layers of fertilized egg ovum sheet being put into the hatchery is hatched, and adopts the mode of hydrostatic, continuous charge and 16 ℃ of water temperature Constant temperature hatchs;
(2) the every morning 8:30～10:00 of sunshine is being arranged, 2 layers of fertilized egg ovum sheet are taken out from the hatchery and are exposed in the air, accepting shining of sunshine shines, every photograph solarization was carried out seawater current to fertilized egg ovum sheet in 15 minutes and is sprayed stimulation, it is 60 seconds that each current spray stimulation time, be 1.5 hour according to the solarization time every day, then 2 layers of fertilized fish roe sheet put back to and continued hatching in the hatchery;
The brooding time of (3) 2 layers of fertilized egg ovum sheet in 16 ℃ of hatcheries is 18～20 days, newly hatched larvae focuses mostly in net cage edge and bottom, then active and move about in water body at the middle and upper levels gradually, when waiting to reach certain density, in time prelarva is shifted out the hatchery and cultivate to cultivating the pond.
According to solarization time every morning 8:30～10:00 by the end of October～by the end of November because this section period sunshine slightly a little less than, be conducive to reach according to the effect of shining.
The present invention has obviously improved the incubation rate of greenling fertilized egg, has improved production capacity, for the scale seed breeding is laid a good foundation.
The above only is preferred embodiment of the present invention, is not to be the restriction of the present invention being made other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not break away to any simple modification, equivalent variations and remodeling that above embodiment does, still belongs to the protection domain of technical solution of the present invention according to technical spirit of the present invention.
Claims (5)
1. a method that improves greenling fertilized egg hatching rate is characterized in that, the artificial incubation step of described method is:
(1) incubation net cage of fertilized egg ovum sheet being put into the hatchery is hatched, and adopts the mode of hydrostatic, continuous charge and 16 ℃ of water temperature Constant temperature hatchs;
(2) having in the every day of sunshine, fertilized egg ovum sheet is taken out from the hatchery and is exposed in the air, accepting shining of sunshine shines, every 10～15 minutes fertilized egg ovum sheets of solarization that shine carry out a seawater current sprinkling stimulation, it is 30～60 seconds that each current spray stimulation time, be 1～1.5 hour according to the solarization time every day, then puts back to and continue hatching in the hatchery;
(3) brooding time of fertilized egg ovum sheet in 16 ℃ of hatcheries is 18～20 days, newly hatched larvae focuses mostly in net cage edge and bottom, then active and move about in water body at the middle and upper levels gradually, when waiting to reach certain density, in time prelarva is shifted out the hatchery and cultivate to cultivating the pond.
2. a kind of method that improves greenling fertilized egg hatching rate according to claim 1 is characterized in that, according to shine selection of time every year by the end of October～by the end of November the morning 8:30～10:00 time in.
3. a kind of method that improves greenling fertilized egg hatching rate according to claim 1 is characterized in that, according to shine selection of time every year by the end of October～by the end of November every afternoon 14:30～16:00 time in.
4. a kind of method that improves greenling fertilized egg hatching rate according to claim 1 is characterized in that, described fertilized egg ovum sheet is individual layer.
5. a kind of method that improves greenling fertilized egg hatching rate according to claim 1 is characterized in that, described fertilized egg ovum sheet is 2～3 layers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011102393853A CN102318574B (en) | 2011-08-19 | 2011-08-19 | Method for improving greenling fertilized egg hatching rate |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011102393853A CN102318574B (en) | 2011-08-19 | 2011-08-19 | Method for improving greenling fertilized egg hatching rate |
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| Publication Number | Publication Date |
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| CN102318574A CN102318574A (en) | 2012-01-18 |
| CN102318574B true CN102318574B (en) | 2013-03-27 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107410111A (en) * | 2017-06-06 | 2017-12-01 | 明光市裕阳农业有限公司 | A kind of cultural method for improving grass carp survival rate |
| CN108935245A (en) * | 2018-06-19 | 2018-12-07 | 山东省海洋生物研究院 | A kind of efficient hatching method of viscid egg fish oosperm |
| CN113728951A (en) * | 2021-09-30 | 2021-12-03 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Hexagrammos otakii large-scale insemination and incubation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101773082B (en) * | 2010-01-25 | 2011-12-14 | 山东省海水养殖研究所 | Method for artificial fertilization and hatching of hexagrammos otakii |
| CN102077802B (en) * | 2010-12-31 | 2012-09-05 | 烟台大学 | Parent fish breeding and hatching method for greenlings |
| CN102106282B (en) * | 2011-01-17 | 2012-10-17 | 山东省海水养殖研究所 | Artificial inseminating and hatching method of high-viscosity fish eggs |
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