CN102308714B - Artificial cultivation method for increasing content of salvianolic acid ingredient - Google Patents
Artificial cultivation method for increasing content of salvianolic acid ingredient Download PDFInfo
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- CN102308714B CN102308714B CN 201110109973 CN201110109973A CN102308714B CN 102308714 B CN102308714 B CN 102308714B CN 201110109973 CN201110109973 CN 201110109973 CN 201110109973 A CN201110109973 A CN 201110109973A CN 102308714 B CN102308714 B CN 102308714B
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Abstract
The invention discloses an artificial cultivation method for increasing content of a salvianolic acid ingredient. In the method disclosed by the invention, a step of spraying an aqueous solution of methyl jasmonate is only added on the basis of the original conventional production and planting process, and the sprayed aqueous solution is prepared by adding 0.015-0.40g of chemically pure or industrially pure commercial methyl jasmonate into 100L of clean water and then stirring to completely dissolving the methyl jasmonate. The method is simple to implement and convenient to operate, can be mastered and easily accepted by common medicinal herb collectors and is convenient to popularize and apply, the investment is less, the harvest is great, the total content of the salvianolic acid ingredient in roots, stems and blades of a red-rooted sage plant can be greatly increased, specifically, the total content of the salvianolic acid ingredient in the roots of the red-rooted sage plant is increased to about 9% from about 5% and is nearly twice of the original total content of the salvianolic acid ingredient, the total content of the salvianolic acid ingredient in the stems and blades of the salvia miltiorrhiza plant is increased to about 4% from about 1.6% and is nearly more than four times of the original total content of the salvianolic acid ingredient, thus the medical value of the roots of the red-rooted sage plant is higher and the stems and blades of the red-rooted sage also have values in development and utilization.
Description
Technical field
The present invention relates to a kind of Chinese herbal medicine cultivation technology, i.e. the cultivation method of the red sage root.
Background technology
The red sage root is a labiate, is used as medicine with root usually, and its active ingredient comprises fat-soluble and water-soluble two big types.Liposoluble constituent mainly is diterpene quinone tanshinone, Cryptotanshinone etc., and water soluble ingredient is mainly phenolic acid compound danshensu, protocatechualdehyde, danshinolic acid etc., and danshinolic acid has oxidation resistant effect.Application in cosmetics and health products more and more widely, at treatment angiocardiopathy, chronic hepatitis, anti-hepatic fibrosis, antiatherosclerosis with improve aspect such as memory dysfunction and bringing into play important effect especially.Particularly more and more deep to the application study of its phenolic acid class composition in recent years, its purposes is also more and more, and demand is also increasing.Demand promotion is produced also to stimulate and is excavated, and large batch of year after year excavating makes wild resource receive heavy damage, and some place is endangered, the output continuous decrease.But because salvia miltiorrhiza adaptability is not strong, to having relatively high expectations of cultivation, output is little; In addition, the red sage root mainly contain the effective constituent salvianolic acid, content is high in its root; The output of its leaf is also not little, but the content of the danshinolic acid class composition in the leaf is lower, does not have value of exploiting and utilizing.In sum, on the one hand the demand of the red sage root is increasing, and the output of the red sage root is limited on the other hand, so improve the content of its effective ingredient or the comprehensive utilization of its blade more and more receives publicity.
Summary of the invention
The object of the invention provides a kind of red sage root crop artificial cultivation method that improves the phenolic acid class component content in the salvia miltiorrhiza.
Technical scheme of the present invention is:
A kind of artificial cultivation method that improves the salvianolic acid component content, comprise choosing ground put in order and apply fertilizer to the subsoil, propagation method, field management, root outer spraying, extermination of disease and insect pest step, the concrete grammar of each step is following:
One, choosing ground, whole ground, apply fertilizer to the subsoil
Select deep, the open-textured sand of soil layer, avoid clay and saline land, avoid continuous cropping;
Treat whole ground, autumn crop results back, execute farmyard manure for every mu and make base manure for 2000～3000 kilograms, deep plough, rake, make 1.2～1.5 meters wide furrow, 0.3～0.6 meter of furrow width, furrow are high 20～40 centimetres, in order to draining.
Two, propagation method
(1) root division
When gathering in the crops autumn, reserve the part plot and do not dig, to digging between 1 year 2～March, selecting diameter is 0.7～l.0 centimetre; Stalwartness, no damage by disease and insect, the red root of color of the leather are made kind of a root, get the strong part of epimere sprouting ability in the root bar, are cut into long about 5 centimetres sections, open the cave; Dark 5～7 centimetres of cave, 1～2 section of root segment is put in every cave, tiltedly puts; The upper end is kept up,, plant about 3 centimetres of back earthing with digging with cutting with planting;
(2) cottage propagation
In the plant strain growth most productive period in 4～May, get red sage root terrestrial stem, be cut into the segment about 7～10 centimetres; Wipe out lower blade, upper blade cuts off l/2, on ready-made seedbed, presses 10 centimetres of seeding row spacing 6 cm x then; Oblique cutting buries 1/2～1/3, accomplishes to water to shade after and preserve moisture with cutting with inserting; Treat can transplant the land for growing field crops when root grows to 3 centimetres of left and right sides, 30 centimetres of plantations of seeding row spacing 25 cm x are pressed in the land for growing field crops.
Three, field management
Intertill and clean tillage: root division person, often because of blinding is too thick, obstruction is emerged, and therefore, will look into seedling when come up 3～April, and is too thick or table soil hardens because of blinding as finding, should be with excavating on the cave.Carry out the intertill and clean tillage first time during 5～6 centimetres of heights of seedling, intertillage is shallow, avoids hindering root, for the second time in June, carries out in 7～August for the third time, and envelope stops intertillage behind the ridge;
Topdress: combine intertill and clean tillage to topdress 2 times; Every mu imposes the people and animals' liquid dung 4000kg that becomes thoroughly decomposed for the first time; And add potassium chloride 3～5kg, and carrying out intertill and clean tillage second time to early July by the end of June as the envelope row is last, every mu of while imposes 4000～5000 kilograms of people and animals' liquid dungs of becoming thoroughly decomposed;
Irrigation and drainage: the seeding stage will often keep ground moistening, in order to emerging and growth of seedling, wants timely draining rainy season, in order to avoid mashed root.
Four, root outer spraying
(1) spray aqueous solution preparation: get commercially available technical pure or chemical pure methyl jasmonate 0.015g～0.40g, add in the 100L clean water, stirring is dissolved it fully, processes the methyl jasmonate aqueous solution;
The said preferred preparation that sprays the aqueous solution: get commercially available technical pure or chemical pure methyl jasmonate 0.08g～0.30g, add in the 100L clean water, stirred 20～30 minutes, it is dissolved fully, process the methyl jasmonate aqueous solution;
The said the best preparation that sprays the aqueous solution: get commercially available technical pure or chemical pure methyl jasmonate 0.20g, add in the 100L clean water, stirred 20～30 minutes, it is dissolved fully, process the methyl jasmonate aqueous solution.
(2) spray the time: selection sprays the time in the morning or sprayed up to 5 o'clock afternoon 3;
(3) spray season and number of times: in May～September, different local climate is different, with red sage root plant long have 10 with blade to plant base leaf begin withered and yellow till, use altogether 8～15 times, used once in 8～15 days at every interval;
(4) spraying method: the methyl jasmonate aqueous solution for preparing more than inciting somebody to action is packed in the agroatomizer; The methyl jasmonate aqueous solution is sprayed on the blade of red sage root plant through sprayer; All to spray from both direction up and down so that the blade upper and lower surface of red sage root plant all or major parts evenly be covered with the methyl jasmonate aqueous solution and be advisable.
Five, the extermination of disease and insect pest
Root rot: note draining rainy season; Crop rotation; Their early stage is with 800～1000 times of liquid pourings of 50% topsin;
Root knot nematode disease: rice field-upland field rotation; With 2～3 kilograms of 80% nemagon, add the water double centner, evenly applied in the soil in preceding 15 days and earthing in plantation;
China dodder: in time root out diseased plant vegetative period; Remove the dodder seed;
Cotton bollworm: spray 1500 times of liquid of 50% phoxim emulsifiable concentrate or 600 times of liquid of 50% sevin in the flower bud phase;
Three-spotted plusia: the juvenile stage worm sprays with 800 times of liquid of 90% chlorophos or 1500 times of liquid of 4O% flolimat.
According to actual conditions: in the choosing ground process, should select not plant more than at least 1 year the soil of the red sage root; The root outer spraying time should be avoided the weather that rains.
Above-mentioned one, choosing ground, whole ground, apply fertilizer to the subsoil; Two, propagation method; Three, field management; Five, the extermination of disease and insect pest all is methods that existing conventional is produced plantation.
Beneficial effect of the present invention:
The present invention has only increased the step that sprays the methyl jasmonate aqueous solution original conventional production on the basis of planting, and it implements simple, and is easy to operate; Common medicinal herb grower can both grasp, and is easy to accepted by the medicinal herb grower, and is easy to utilize; Drop into seldom, gather in the crops greatlyyer, can significantly improve the total content of the salvianolic acid composition in red sage root and the red sage root plant cauline leaf; The total content of the salvianolic acid composition in the red sage root is brought up to about 9% from about 5%; Improve nearly 1 times, the total content that makes the salvianolic acid composition in the red sage root plant cauline leaf is brought up to about 4% from about 1.6%, improves about 3 times; Thereby make the medical value of red sage root bigger, make red sage root stem and blade also have value of exploiting and utilizing.The medical value of red sage root has improved on the one hand, and utilizable thing is many, can prevent effectively excessively to excavate, and has protected wild resource, has prevented water and soil destruction, has protected ecotope; Needs have been satisfied in medicine and cosmetics, the health products on the other hand, for human beings'health and long-life have been made contribution as raw material; Use stem of passing into disuse originally and leaf on the one hand again, no longer burn, both protected environment, improved the income at plantation family again greatly, have good economic benefits and social benefit as refuse.
Embodiment
Embodiment 1:
The present invention improves the artificial cultivation method of salvianolic acid component content; Comprise choosing ground put in order and apply fertilizer to the subsoil, propagation method, field management, root outer spraying, extermination of disease and insect pest step; Wherein dig up the roots outside outer the spraying, all be the choosing ground of said existing routine above adopting put in order with the method that applies fertilizer to the subsoil, the production of propagation method, field management, the extermination of disease and insect pest is planted.The soil of the red sage root is preferably selected not plant more than at least 1 year in choosing ground.
Key of the present invention is in the root outer spraying step, and its concrete operations are following:
(1) sprays aqueous solution preparation: get commercially available technical pure or chemical pure methyl jasmonate 0.15g, add in the 100L clean water, stirred 25 minutes, it is dissolved fully, process the methyl jasmonate aqueous solution with wooden stick or sticking plaster;
(2) spray the time: selection sprays the time in the morning or sprayed up to 5 o'clock afternoon 3, should avoid the weather that rains;
(3) spray season and number of times: in May～September, different local climate is different, with red sage root plant long have 10 with blade to plant base leaf begin withered and yellow till, use altogether 8～15 times, used once in 8～15 days at every interval;
(4) spraying method: the methyl jasmonate aqueous solution for preparing more than inciting somebody to action is packed in the agroatomizer; The methyl jasmonate aqueous solution is sprayed on the blade of red sage root plant through sprayer; All to spray from both direction up and down so that the blade upper and lower surface of red sage root plant all or major parts evenly be covered with the methyl jasmonate aqueous solution and be advisable.
Embodiment 2:
Different with embodiment 1 is; The said preparation that sprays the aqueous solution: get commercially available technical pure or chemical pure methyl jasmonate 0.20g, add in the 100L clean water, stirred 30 minutes with wooden stick or sticking plaster; It is dissolved fully, process the methyl jasmonate aqueous solution; Other are identical with embodiment 1.
Embodiment 3
Different with embodiment 1 is the said preparation that sprays the aqueous solution: get commercially available technical pure or chemical pure methyl jasmonate 0.10g, add in the 100L clean water, stirred 20 minutes with wooden stick or sticking plaster, it is dissolved fully, process the methyl jasmonate aqueous solution; Other are identical with embodiment 1.
Method practical application performance of the present invention: the total content of the salvianolic acid composition in the red sage root is brought up to about 9% from about 5%; The total content of the salvianolic acid composition in the red sage root plant cauline leaf is brought up to about 4% from about 1.6%; Improve the quality of the red sage root and the output of active ingredient, really accomplished good quality and high output.
Claims (6)
1. artificial cultivation method that improves the salvianolic acid component content, comprise choosing ground put in order and apply fertilizer to the subsoil, propagation method, field management, root outer spraying, extermination of disease and insect pest step, it is characterized in that the method for said root outer spraying is following:
(1) spray aqueous solution preparation: get commercially available technical pure or chemical pure methyl jasmonate 0.015g～0.40g, add in the 100L clean water, stirring is dissolved it fully, processes the methyl jasmonate aqueous solution;
(2) spray the time: selection sprays the time in the morning or sprayed up to 5 o'clock afternoon 3;
(3) spray season and number of times: in May～September, different local climate is different, with red sage root plant long have 10 with blade to plant base leaf begin withered and yellow till, use altogether 8～15 times, used once in 8～15 days at every interval;
(4) spraying method: the methyl jasmonate aqueous solution that above-mentioned (1) is prepared is packed in the agroatomizer; The methyl jasmonate aqueous solution is sprayed on the blade of red sage root plant through sprayer; Both direction all will spray up and down, makes the whole perhaps major parts of blade upper and lower surface of red sage root plant evenly be covered with the methyl jasmonate aqueous solution.
2. the artificial cultivation method of raising salvianolic acid component content according to claim 1; It is characterized in that said (1) sprays aqueous solution preparation: get commercially available technical pure or chemical pure methyl jasmonate 0.08g～0.30g; Add in the 100L clean water; Stirred 20～30 minutes, it is dissolved fully, process the methyl jasmonate aqueous solution.
3. the artificial cultivation method of raising salvianolic acid component content according to claim 1; It is characterized in that said (1) sprays aqueous solution preparation: get commercially available technical pure or chemical pure methyl jasmonate 0.20g; Add in the 100L clean water; Stirred 20～30 minutes, it is dissolved fully, process the methyl jasmonate aqueous solution.
4. the artificial cultivation method of raising salvianolic acid component content according to claim 1 is characterized in that: fertilization time should be avoided the weather that rains.
5. the artificial cultivation method of raising salvianolic acid component content according to claim 1; It is characterized in that: put in order to described choosing with apply fertilizer to the subsoil, propagation method, field management, extermination of disease and insect pest step, its implementation method all adopts conventional method of producing plantation.
6. according to the artificial cultivation method of claim 1 or 5 described raising salvianolic acid component contents, it is characterized in that: choosing ground is the soil of not planting the red sage root more than 1 year.
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Families Citing this family (7)
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| CN103262743B (en) * | 2013-06-03 | 2014-08-20 | 四川逢春制药有限公司 | Cultivation method for improving continuous cropping yield and active ingredient content of salvia miltiorrhiza |
| CN103947424A (en) * | 2014-04-28 | 2014-07-30 | 四川逢春制药有限公司 | Rhiaoma iridis tectori planting method capable of increasing yield and contents of effective components |
| CN104871973A (en) * | 2015-05-18 | 2015-09-02 | 天津大学 | Method for accelerating accumulation of saponin in ginseng adventitious roots by adopting two types of inductors |
| CN104920209A (en) * | 2015-05-18 | 2015-09-23 | 天津大学 | Method for taking two inducers to promote ginseng adventitious saponin accumulation |
| CN106376317A (en) * | 2016-08-26 | 2017-02-08 | 李冬琼 | Plantation method of improving content of danshinolic acid in root of red-rooted salvia |
| CN107173008A (en) * | 2017-06-07 | 2017-09-19 | 云南自然谷生物开发有限公司 | Improve the red sage root implantation methods of tanshinone content |
| CN109429964A (en) * | 2018-12-13 | 2019-03-08 | 山东中医药大学 | A kind of implantation methods improving salvia-soluble liposoluble ingredient |
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| CN101491571A (en) * | 2008-01-23 | 2009-07-29 | 上海中医药大学 | Hair root of salvia miltiorrhiza containing various compounds of high content and culturing technique thereof |
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