CN102305823B - 一种甲型副伤寒沙门菌脉冲场凝胶电泳方法 - Google Patents
一种甲型副伤寒沙门菌脉冲场凝胶电泳方法 Download PDFInfo
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- CN102305823B CN102305823B CN201110137040.7A CN201110137040A CN102305823B CN 102305823 B CN102305823 B CN 102305823B CN 201110137040 A CN201110137040 A CN 201110137040A CN 102305823 B CN102305823 B CN 102305823B
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Description
试剂 | μl/胶块 | μl/10胶块 |
纯水 | 180μl | 1800μl |
Spe I酶配套缓冲液 | 20μl | 200μl |
总体积 | 200μl | 2000μl |
试剂 | μl/胶块 | μl/10胶块 |
纯水 | 175μl | 1750μl |
Spe I酶配套缓冲液 | 20μl | 200μl |
Spe I酶(10U/μl) | 5μl | 50μl |
总体积 | 200μl | 2000μl |
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CN201110137040.7A CN102305823B (zh) | 2011-05-25 | 2011-05-25 | 一种甲型副伤寒沙门菌脉冲场凝胶电泳方法 |
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CN201110137040.7A CN102305823B (zh) | 2011-05-25 | 2011-05-25 | 一种甲型副伤寒沙门菌脉冲场凝胶电泳方法 |
Publications (2)
Publication Number | Publication Date |
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CN102305823A CN102305823A (zh) | 2012-01-04 |
CN102305823B true CN102305823B (zh) | 2014-07-30 |
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102643901B (zh) * | 2012-03-31 | 2013-09-25 | 中国疾病预防控制中心传染病预防控制所 | 一种检测甲型副伤寒沙门菌的rt-lamp试剂盒 |
EP3414255B1 (en) * | 2015-11-20 | 2021-09-22 | Washington University | Preparative electrophoretic method for targeted purification of genomic dna fragments |
CN105671166B (zh) * | 2016-03-04 | 2019-01-29 | 杭州贝因美母婴营养品有限公司 | 一种乳源革兰氏阳性菌的脉冲场凝胶电泳分型方法 |
JP2020515250A (ja) | 2017-04-07 | 2020-05-28 | セージ サイエンス, インコーポレイテッド | 統合型電気泳動dna精製を使用する遺伝的構造変動を検出するためのシステムおよび方法 |
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2011
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Non-Patent Citations (6)
Title |
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中国2000--2008年间甲型副伤寒沙门菌脉冲场凝胶电泳和多位点序列分析;韩辉等;《中国预防医学杂志》;20100930;第44卷(第9期);第810-814页 * |
云南省玉溪市甲型副伤寒沙门菌PFGE数据库的建立及其应用;姚颖波等;《疾病监测》;20091030;第24卷(第10期);第770-773 * |
姚颖波等.云南省玉溪市甲型副伤寒沙门菌PFGE数据库的建立及其应用.《疾病监测》.2009,第24卷(第10期),第770-773页. |
我国伤寒和甲型副伤寒沙门菌PFGE的分型研究;陈春霞;《中国优秀硕士学位论文全文数据库 医药卫生科技辑》;20081115(第11期);摘要,第4-7、31、37页 * |
陈春霞.我国伤寒和甲型副伤寒沙门菌PFGE的分型研究.《中国优秀硕士学位论文全文数据库 医药卫生科技辑》.2008,(第11期),摘要,第4-7、24、31、37页. |
韩辉等.中国2000--2008年间甲型副伤寒沙门菌脉冲场凝胶电泳和多位点序列分析.《中国预防医学杂志》.2010,第44卷(第9期),第810-814页. |
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