CN102293321A - Antibiotic-free high live bacteria fermented feed packet for livestock and poultry - Google Patents
Antibiotic-free high live bacteria fermented feed packet for livestock and poultry Download PDFInfo
- Publication number
- CN102293321A CN102293321A CN2011101982757A CN201110198275A CN102293321A CN 102293321 A CN102293321 A CN 102293321A CN 2011101982757 A CN2011101982757 A CN 2011101982757A CN 201110198275 A CN201110198275 A CN 201110198275A CN 102293321 A CN102293321 A CN 102293321A
- Authority
- CN
- China
- Prior art keywords
- livestock
- poultry
- antibiotic
- bag
- fermented feed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
The invention belongs to the field of feed package, and especially relates to an antibiotic-free high live bacteria fermented feed packet for livestock and poultry. The antibiotic-free high live bacteria fermented feed packet for livestock and poultry is composed of a packaging bag and a feed base-material packed in the packaging bag for strain inoculation. A mixed bacterial liquid, which is inoculated according to 10-40% the total weight of the feed base-material with the total bacteria number being larger than 108cfu/g, is composed of anaerobic bacteria and facultative anaerobe. The packaging bag is provided with a unidirectional exhaust valve device. The fermented feed prepared by the adoption of the fermented feed packet contains comprehensive nutrition, is produced by fermentation, has a biological activity with no residual, and will never become resistant to drugs.
Description
Technical field
The invention belongs to the feed package field, relate in particular to the high viable bacteria livestock and poultry of a kind of packed antibiotic-free and use the fermented feed bag.
Technical background
Traditional aquaculture model uses antibiosis usually to control transmission in a large number, causes the medicament residue of livestock product to exceed standard, even resistance, thereby influences the quality of livestock product.Because antibiotic many side effects, particularly the consumer is to the concern of food-safety problem in recent years, and antibiotic use is being forbidden or limited in countries in the world all one after another, and accelerate development and popularization is novel, safe, green feed additive efficiently.
Development along with modern biotechnology, utilize the method for microbial fermentation, in the process of eliminating the feedstuff fraction antigen, microbial metabolism also can produce materials such as a large amount of active plant polypeptide, lactic acid, multiple digestive ferment, and these materials have obtained using widely in field of fodder.
Chinese invention patent application (application number: 201010129568.5, the applying date: 2010-03-23) disclose the preparation method of the composite probiotic fermented feed in livestock technology field, comprise the steps: step 1, get following raw material: yellow maize, wheat flour, rapeseed dregs and dregs of beans; Step 2 with raw material pulverizing that step 1 is got, is mixed afterwards, gets mixed material, adds entry and compound probiotic in mixed material, mix thoroughly, and sealing, fermentation gets finished product feedstuff.
(application number: 200810152411.7 applyings date: 2008-10-21) disclose a kind of method of utilizing microbial fermentation to produce fermented feed containing probiotics for pigs, be made up of following step: (1) cultivates aerobic fungi as producing bacterial classification in the Chinese invention patent application; (2) cultivation of lactic acid producing bacillus; (3) solid-state cultivation: 1. with dregs of beans, cotton dregs, vinegar poor with wheat bran or/and rice bran fully mixes, add water, make mixture; 2. insert the production bacterial classification that step (1) and step (2) make; 3. pile up or pack pile and cultivate, make the pig no-antibiotics feed.
Drawbacks such as but traditional fermentative feedstuff of microbe exists, and bacterial classification is single, technology simple, fermentation is not dark, antigen is removed not thoroughly, the not high and useful viable bacteria of feed active constituent content is few, can not really substitute antibiotic, DeGrain aspect growth promotion and disease preventing and treating.In addition, the time of fermentation is long, and needs the operation place of fermentation and storage, has increased the cost of producing and storing.
Summary of the invention
In order to overcome above-mentioned the deficiencies in the prior art, the purpose of this invention is to provide the high viable bacteria livestock and poultry of a kind of packed antibiotic-free and use the fermented feed bag, adopt fermented feed to pack the fermented feed that is equipped with and have the mixture comprehensive nutrition, and fermentation is come out, biologically active, can be not residual, can not produce drug resistance yet.
In order to realize above-mentioned purpose, the present invention has adopted following technical scheme:
The high viable bacteria livestock and poultry of packed antibiotic-free are used the fermented feed bag, this fermented feed bag is made of packaging bag and the feed base-material that is packaged in the packaging bag inoculation bacterial classification, described feed base-material is the 10-100 order, in feed base-material gross weight is 100%, and corn is that 65%-70%, dregs of beans are that 15%--20%, the cottonseed dregs of rice are 5%-10%, wheat bran 2%-10%, premix 1%-4%; 10%~40% inoculation total bacteria count of pressing feed base-material gross weight is greater than 10
8The mixed bacteria liquid of cfu/g, described mixed bacteria liquid is made of aerobic bacteria and facultative anaerobe; Described packaging bag is provided with unidirectional gas outlet valve apparatus, and unidirectional gas outlet valve apparatus comprises valve seat and valve gap, and the bottom of valve seat is provided with ring shape binding portion, ring shape binding portion and packaging bag inboard bonding; Be provided with first recess in the centre of ring shape binding portion, be provided with the truncated cone-shaped boss in first recess, described valve gap be located on the truncated cone-shaped boss, is formed with internal cavity between described valve gap and the truncated cone-shaped boss, is provided with the silica gel sheet in the internal cavity; And on described valve gap, be provided with venthole and be used to connect outside and internal cavity, the middle part of truncated cone-shaped boss is provided with air admission hole and is used to connect internal cavity and packaging bag inside, the below of described truncated cone-shaped boss is provided with second recess, be provided with cellulose bacteriological filtration sheet in second recess, cellulose bacteriological filtration sheet covers the air admission hole inboard.
As preferably, by weight aerobic bacteria: facultative anaerobe is 40%-50%:50%-60%.
As preferably, above-mentioned aerobic bacteria is selected saccharomycete and bacillus subtilis for use.
As preferably, above-mentioned facultative anaerobe is selected one or both in Bacillus acidi lactici and the streptococcus lactis for use.
As preferably, the PE combined package bag is selected in above-mentioned packaging bag for use, and described unidirectional gas outlet valve apparatus bonding is arranged on the inboard of PE combined package bag.Select 3 layers~5 layers composite membrane for use as preferred again, the above-mentioned used film of PE combined package bag.
As further improvement, the madial wall of above-mentioned valve gap is provided with a plurality of boss, and boss and silica gel sheet offset.
As further improvement, the circumferential madial wall of above-mentioned valve gap is provided with annular skewed slot, and annular skewed slot matches with the truncated cone-shaped boss.
The present invention utilizes aerobic bacteria reproduction speeds such as saccharomycete, bacillus subtilis than fast these characteristics of anaerobic bacteria in the packaging bag that has unidirectional gas outlet valve apparatus, produces the time difference, allows aerobic bacteria ferment earlier, reaches the purpose of the oxygen in a large amount of consumption packaging bags.Simultaneously, utilization has a large amount of bacillus subtilis of powerful fungistatic effect, kill assorted bacterium such as the mould that contains in each component of feedstuff, Escherichia coli, salmonella, reach the purpose of killing assorted bacterium in the packaging bag that has unidirectional gas outlet valve apparatus and the feedstuff wherein.In addition, aerobic fermentation also produces a large amount of lactic acid; These lactic acid, and the environment of the assorted bacterium of anaerobic, nothing are for next step anaerobic fermentation has been created excellent condition.Then, by described anaerobic bacteria (as Bacillus acidi lactici, streptococcus lactis) as, these bacterium to the fermentation of feed, metabolism zymogenesis must be under anaerobic, acid effect competence exertion to ultimate attainment, make the antigen degraded more thorough, it is abundanter to produce enzyme, and plant polypeptide is more, and digestion is more obvious.Employing has the purpose of unidirectional gas outlet valve apparatus, will make the interior material of packaging bag at anaerobic fermentation stage secluding air (oxygen) exactly, and can the carbon dioxide that fermentating metabolism produces be discharged outside the bag by check valve.Adopt method of the present invention in addition, fermentation time shortens greatly, can directly deliver after packing, ferments in fermentation or the user's storage process in the employing logistics progress, has saved the time of fermenting in the production process like this, has saved the occupation of land of fermentation plant.
The time difference of the different generations of the packaging bag that utilization of the present invention has a unidirectional gas outlet valve apparatus and aerobic, anaerobe fermentation time, produce a kind of mixed feed mixture that really has an antibacterial and growth promoting function of antibiotic (as multiple digestive ferment, lactic acid etc. such as the viable bacteria-bacillus subtilis of antibacterial peptide, high-load, protease), this mixture comprehensive nutrition, and fermentation is come out, biologically active, can be not residual, can not produce drug resistance yet.Can urge growth of animals or poultry; Can prevent and treat livestock and poultry disease of digestive system (as control diarrhoea); Improve immunity; Improve food conversion ratio; Improve palatability; Improve livestock and poultry meat matter; Reduce environmental pollution etc.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Fig. 2 is the structural representation of unidirectional gas outlet valve apparatus.
The specific embodiment
A kind of high viable bacteria livestock and poultry of antibiotic-free that have unidirectional gas outlet valve apparatus are used fermented feed and preparation method thereof.
1) of the present invention consisting of: fatten livestock and poultry material: corn is 66%, dregs of beans 18%, the cottonseed dregs of rice are 8%, wheat bran 6%, premix 2%.
2) described preparation method: above-mentioned raw materials is crushed to 30 orders, mixes standby by formula rate; Saccharomycete, bacillus subtilis are inoculated into respectively in the fermentation tank that has aerating device, in proper culture medium, spread cultivation 18-24 hour; Bacillus acidi lactici, streptococcus lactis are inoculated into respectively in the fermentation tank that does not have aerating device, anaerobic spread cultivation 44-48 hour in proper culture medium again.To be detectedly all reach 10
9During cfu/g density, in saccharomycete: bacillus subtilis: Bacillus acidi lactici: streptococcus lactis=20-25%:20-25%:25-30%:25:30% ratio mixing is diluted to greater than 10 with sterilized water again
9Cfu/g density is then inoculated total bacteria count greater than 10 in 25% of material ratio
8The mixed bacteria liquid of cfu/g packs with the packaging bag that has unidirectional gas outlet valve apparatus more than 5 layers in mixing each standby feedstuff component, and static solid state fermentation is more than 5 days.
A kind of high viable bacteria livestock and poultry of antibiotic-free that have unidirectional gas outlet valve apparatus are used fermented feed and preparation method thereof.
1) of the present invention consisting of: newborn newborn animal fowl material: corn is 66%, dregs of beans 18%, the cottonseed dregs of rice are 8%, wheat bran 4%, premix 4%.
2) described preparation method: above-mentioned raw materials is crushed to 60 orders, mixes standby by formula rate; Saccharomycete, bacillus subtilis are inoculated into respectively in the fermentation tank that has aerating device, in proper culture medium, spread cultivation 18-24 hour; Bacillus acidi lactici, streptococcus lactis are inoculated into respectively in the fermentation tank that does not have aerating device, anaerobic spread cultivation 44-48 hour in proper culture medium again.To be detectedly all reach 10
9During cfu/g density, in saccharomycete: bacillus subtilis: Bacillus acidi lactici: streptococcus lactis=20-25%:20-25%:25-30%:25:30% ratio mixing is diluted to greater than 10 with sterilized water again
9Cfu/g density is then inoculated total bacteria count greater than 10 in 25% of material ratio
8The mixed bacteria liquid of cfu/g packs with the packaging bag that has unidirectional gas outlet valve apparatus more than 5 layers in mixing each standby feedstuff component, and static solid state fermentation is more than 5 days.
As shown in Figure 1, the packaging bag of the unidirectional gas outlet valve apparatus among the above embodiments 1 and the embodiment 2, the PE combined package bag is selected in this packaging bag for use, and used film is selected 5 layers of composite membrane 1 for use.As shown in Figure 2, described unidirectional gas outlet valve apparatus comprises valve seat 2 and valve gap 3, and the bottom of valve seat 2 is provided with ring shape binding portion 14, and ring shape binding portion 14 passes through hot adhesion with the inboard of packaging bag; Be provided with in first recess, 9, the first recesses 9 in the centre of ring shape binding portion 14 and be provided with truncated cone-shaped boss 12, described valve gap 3 be located on the truncated cone-shaped boss 12, and valve gap 3 circumferential madial walls are provided with annular skewed slot 13, and annular skewed slot 13 matches with truncated cone-shaped boss 12.Be formed with internal cavity 10 between described valve gap 3 and the truncated cone-shaped boss 12, be provided with silica gel sheet 5 in the internal cavity 10, and be provided with a plurality of boss 8 at the madial wall of valve gap 3, boss 8 offsets with silica gel sheet 5.
As shown in Figure 1, described valve gap 3 is provided with venthole 6 and is used for connecting outside and internal cavity 10, the middle part of truncated cone-shaped boss 12 is provided with air admission hole 7 and is used to connect internal cavity 10 and packaging bag inside, the below of described truncated cone-shaped boss 12 is provided with second recess 11, be provided with cellulose bacteriological filtration sheet 4 in second recess 11, cellulose bacteriological filtration sheet 4 covers air admission hole 7 inboards.
The feedstuff that to mix bacterial classification when using joins in the packaging bag, seals, and the film on the venthole 6 is poked, and gets final product.
Claims (8)
1. the high viable bacteria livestock and poultry of packed antibiotic-free are used the fermented feed bag, it is characterized in that: this fermented feed bag is made of packaging bag and the feed base-material that is packaged in the packaging bag inoculation bacterial classification, described feed base-material is the 10-100 order, in feed base-material gross weight is 100%, and corn is that 65%-70%, dregs of beans are that 15%-20%, the cottonseed dregs of rice are 5%-10%, wheat bran 2%-10%, premix 1%-4%; 10%~40% inoculation total bacteria count of pressing feed base-material gross weight is greater than 10
8The mixed bacteria liquid of cfu/g, described mixed bacteria liquid is made of aerobic bacteria and facultative anaerobe; Described packaging bag is provided with unidirectional gas outlet valve apparatus, and unidirectional gas outlet valve apparatus comprises valve seat (2) and valve gap (3), and the bottom of valve seat (2) is provided with ring shape binding portion (14), and ring shape binding portion (14) is inboard bonding with packaging bag; Be provided with first recess (9) in the centre of ring shape binding portion (14), be provided with truncated cone-shaped boss (12) in first recess (9), described valve gap (3) be located on the truncated cone-shaped boss (12), be formed with internal cavity (10) between described valve gap (3) and the truncated cone-shaped boss (12), be provided with silica gel sheet (5) in the internal cavity (10); And on described valve gap (3), be provided with venthole (6) and be used for connecting outside and internal cavity (10), the middle part of truncated cone-shaped boss (12) is provided with air admission hole (7) and is used to connect internal cavity (10) and packaging bag inside, the below of described truncated cone-shaped boss (12) is provided with second recess (11), be provided with cellulose bacteriological filtration sheet (4) in second recess (11), cellulose bacteriological filtration sheet (4) covers the inboard of air admission hole (7).
2. the high viable bacteria livestock and poultry of packed antibiotic-free according to claim 1 are used the fermented feed bag, it is characterized in that: by weight aerobic bacteria: facultative anaerobe is 40%-50%:50%-60%.
3. the high viable bacteria livestock and poultry of packed antibiotic-free according to claim 1 and 2 are used the fermented feed bag, and it is characterized in that: aerobic bacteria is selected saccharomycete and bacillus subtilis for use.
4. the high viable bacteria livestock and poultry of packed antibiotic-free according to claim 1 and 2 are used the fermented feed bag, and it is characterized in that: facultative anaerobe is selected one or both in Bacillus acidi lactici and the streptococcus lactis for use.
5. the high viable bacteria livestock and poultry of packed antibiotic-free according to claim 1 are used the fermented feed bag, and it is characterized in that: the PE combined package bag is selected in packaging bag for use, and described unidirectional gas outlet valve apparatus bonding is arranged on the inboard of PE combined package bag.
6. the high viable bacteria livestock and poultry of packed antibiotic-free according to claim 5 are used the fermented feed bag, it is characterized in that: the used film of PE combined package bag (1) is selected 3 layers~5 layers composite membrane for use.
7. the high viable bacteria livestock and poultry of packed antibiotic-free according to claim 1 are used the fermented feed bag, it is characterized in that: the madial wall of valve gap (3) is provided with a plurality of boss (8), and boss (8) offsets with silica gel sheet (5).
8. the high viable bacteria livestock and poultry of packed antibiotic-free according to claim 1 are used the fermented feed bag, it is characterized in that: valve gap (3) circumferentially madial wall is provided with annular skewed slot (12), and annular skewed slot (12) matches with truncated cone-shaped boss (12).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2011101982757A CN102293321A (en) | 2011-07-15 | 2011-07-15 | Antibiotic-free high live bacteria fermented feed packet for livestock and poultry |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2011101982757A CN102293321A (en) | 2011-07-15 | 2011-07-15 | Antibiotic-free high live bacteria fermented feed packet for livestock and poultry |
Publications (1)
Publication Number | Publication Date |
---|---|
CN102293321A true CN102293321A (en) | 2011-12-28 |
Family
ID=45354203
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2011101982757A Pending CN102293321A (en) | 2011-07-15 | 2011-07-15 | Antibiotic-free high live bacteria fermented feed packet for livestock and poultry |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102293321A (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103918886A (en) * | 2014-04-21 | 2014-07-16 | 广州东凌粮油股份有限公司 | Method for producing high-activity fermented soybean meal |
CN104186924A (en) * | 2014-08-01 | 2014-12-10 | 福建龙岩金和动物饲料有限公司 | Automatic production process of environment-friendly nonreactive fermented feed |
CN105394333A (en) * | 2015-09-29 | 2016-03-16 | 何建国 | Microorganism-containing element for replacing antibiotics and hormones in breeding |
CN105614021A (en) * | 2015-12-28 | 2016-06-01 | 杨彦红 | Method and equipment for drying and fermenting feed at low temperature |
CN106858038A (en) * | 2017-02-21 | 2017-06-20 | 内蒙古汉恩生物科技有限公司 | A kind of composite probiotic fermented feed and its manufacturing process |
CN110810622A (en) * | 2018-08-14 | 2020-02-21 | 张金岳 | Method for producing biological protein fermentation-fermented feed by applying small molecular kinetic water and special fermentation-fermented bag |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1356242A (en) * | 2000-12-06 | 2002-07-03 | 戈格里奥·卢格米兰公司 | Selective exhaust Valve for container of product with fragrance or smell (such as coffee) |
CN101352197A (en) * | 2007-07-27 | 2009-01-28 | 严明 | Method for producing fermented feed and anaerobic fermentation bag thereof |
CN101611919A (en) * | 2006-01-27 | 2009-12-30 | 北京晟亚育达生物科技有限公司 | Fermentation packaging bag |
CN201709370U (en) * | 2010-04-27 | 2011-01-19 | 刘世红 | Feedstuff fermentation sealing bag with one-way valve |
-
2011
- 2011-07-15 CN CN2011101982757A patent/CN102293321A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1356242A (en) * | 2000-12-06 | 2002-07-03 | 戈格里奥·卢格米兰公司 | Selective exhaust Valve for container of product with fragrance or smell (such as coffee) |
CN101611919A (en) * | 2006-01-27 | 2009-12-30 | 北京晟亚育达生物科技有限公司 | Fermentation packaging bag |
CN101352197A (en) * | 2007-07-27 | 2009-01-28 | 严明 | Method for producing fermented feed and anaerobic fermentation bag thereof |
CN201709370U (en) * | 2010-04-27 | 2011-01-19 | 刘世红 | Feedstuff fermentation sealing bag with one-way valve |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103918886A (en) * | 2014-04-21 | 2014-07-16 | 广州东凌粮油股份有限公司 | Method for producing high-activity fermented soybean meal |
CN104186924A (en) * | 2014-08-01 | 2014-12-10 | 福建龙岩金和动物饲料有限公司 | Automatic production process of environment-friendly nonreactive fermented feed |
CN105394333A (en) * | 2015-09-29 | 2016-03-16 | 何建国 | Microorganism-containing element for replacing antibiotics and hormones in breeding |
CN105614021A (en) * | 2015-12-28 | 2016-06-01 | 杨彦红 | Method and equipment for drying and fermenting feed at low temperature |
CN106858038A (en) * | 2017-02-21 | 2017-06-20 | 内蒙古汉恩生物科技有限公司 | A kind of composite probiotic fermented feed and its manufacturing process |
CN110810622A (en) * | 2018-08-14 | 2020-02-21 | 张金岳 | Method for producing biological protein fermentation-fermented feed by applying small molecular kinetic water and special fermentation-fermented bag |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102415480B (en) | Microbial liquid premix and preparation method thereof | |
CN102696860B (en) | Highly efficient and low-cost microbiological feed proteins based on vinegar residue and miscellaneous meal | |
CN103621766A (en) | Preparation method and application of biological feed additive with nutrition and immunocompetence | |
CN103098986B (en) | Beneficial microorganism antibiont-free feed and preparation method thereof | |
CN103110033A (en) | Pig feed additive and preparation method thereof | |
CN102293321A (en) | Antibiotic-free high live bacteria fermented feed packet for livestock and poultry | |
CN103125778A (en) | Poultry feed additive and preparation method thereof | |
CN103141692A (en) | Feed additive for ruminant animals and preparation method thereof | |
CN102687792A (en) | Feeding microecologic preparation based on beer grains and rice bran meal | |
CN103627656A (en) | Solid state fermentation method of mixed bacteria of clostridium butyricum and bacillus coagulans | |
CN101986861A (en) | Anaerobic effect type multipurpose feed fermentation agent | |
CN105166422A (en) | Biological fermentation feed for accelerating pig growth and preparation method thereof | |
CN100998369A (en) | Bo-gen double micro-organism protein forage | |
CN105394332A (en) | Chicken manure containing fermented protein feed and preparation method thereof | |
CN101836688B (en) | Preparation method of antibiotic-free microbial fermentation feed | |
CN101756024A (en) | Preparation method of probiotic feed additive | |
CN102754744A (en) | Multi-bacteria fermented feed capable of improving pork tenderness | |
CN107712284A (en) | A kind of feed fermentation agent | |
CN102919514B (en) | Preparation method of feed protein peptide without probiotic bacteria activity resistance | |
CN102578441B (en) | Method for producing silage using compound microorganism fermentation straws | |
CN106721278A (en) | Microbial fermentation child care phase piglet liquid-state feed and preparation method and application | |
CN106889307A (en) | A kind of production technology of biological feedstuff of stalk | |
CN109123076A (en) | A kind of production method of livestock and poultry vitamin B2 auxotype probiotics | |
CN102940145A (en) | Preparation method of fermentation aquatic health care feed | |
CN103146616B (en) | A kind of method and application preparing compound lactobacillus preparation |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C12 | Rejection of a patent application after its publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20111228 |