CN102283252A - Composition disinfectant containing matrine and humic acid and preparation method thereof - Google Patents
Composition disinfectant containing matrine and humic acid and preparation method thereof Download PDFInfo
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- CN102283252A CN102283252A CN2011102545930A CN201110254593A CN102283252A CN 102283252 A CN102283252 A CN 102283252A CN 2011102545930 A CN2011102545930 A CN 2011102545930A CN 201110254593 A CN201110254593 A CN 201110254593A CN 102283252 A CN102283252 A CN 102283252A
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- disinfectant
- matrine
- bactericide
- humus
- deionized water
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Abstract
The invention discloses a composition disinfectant containing matrine and humic acid. The disinfectant provided by the invention utilizes purely natural component raw materials including a plant source, a mineral source and the like to effectively prevent and treat the apple tree canker; the production process of the disinfectant is harmless to operators and the plant does not generate phytotoxicity and drug resistance after the disinfectant is applied to the plant so that the disinfectant does not have the use limit; the disinfectant has the comprehensive functions including low toxin, no residues, environmental security, no resistances and the like, so that the disinfectant satisfies the green or organic production standard requirements.
Description
Technical field
The present invention relates to a kind of bactericide and preparation method thereof, more specifically, the present invention relates to a kind of composition fungicide that contains matrine and humic acid and preparation method thereof.
Background technology
Apple valsa canker (Valsa mail Miyabe et Yamada) is a kind of widely distributed worldwide disease, also is on China produces serious a kind of disease to take place generally and endangers.In recent years, incidence trend is on the rise, and generally speaking, bearing tree (10 years) incidence of disease is about 40%, and severe patient can reach more than 85%, the lighter's underproduction, and weight person ruins the garden, and is very big to the yield and quality influence of apple.Therefore, how it is is effectively prevented and treated, become a major issue that needs to be resolved hurrily in the current apple production.The common chemical preparation is prevented and treated apple canker, and its defective is to control " table ", can not control " basis ".Chemical preservative uses the back wound healing poor, and residue of pesticide exceed standard, and often causes tree body immunity degradation.Usually be to take place to control, 1 year more than 1 year heavy phenomenon every year every year.Not only strengthen the orchard worker and invest worker's cost, and residue of pesticide often exceed standard, apple production, quality and ecotope are caused certain harm.
Summary of the invention
In order to overcome above-mentioned defective, the invention provides a kind of composition fungicide that contains matrine and humic acid, its raw material comprises: 0.01-5.0wt% matrine, 0.1-20.0wt% humus, 0.1-5.0wt% bleeding agent and balance of deionized water.
As an optimal technical scheme of the present invention, the content of each raw material is: matrine 0.3wt%, humus 2.7wt%, bleeding agent 2.0wt% and balance of deionized water.
The present invention also provides the preparation method of this bactericide, and it may further comprise the steps:
(1) chooses matrine, humus, bleeding agent and deionized water in proportion;
(2) in reactor, add deionized water, humus is added by proportioning, fully stir, it is dissolved fully, obtain solution a;
(3) in solution a, add matrine, fully stir, it is mixed, obtain bactericide stoste b;
(4) in bactericide stoste b, add bleeding agent, obtain bactericide of the present invention.
The beneficial effect of technical scheme of the present invention is: the technology of the present invention adopts pure natural components raw materials such as plant resource and mineral sources, to the apple valsa canker control effectively, production process is harmless to operating personnel, can not produce poisoning and pesticide resistance to plant after the dispenser, does not have the restriction of use; Have low toxicity, noresidue, environmentally safe, do not produce comprehensive effect such as resistance, meet green or organic production standard requirement.
Embodiment
Below in conjunction with specific embodiment the present invention is described in further detail:
Embodiment 1
Ratio in matrine 0.3wt%, humus 2.7wt%, bleeding agent 2.0wt% and deionized water 95wt% is chosen matrine, humus, bleeding agent and deionized water; In reactor, add deionized water, humus is added by proportioning, fully stir, it is dissolved fully, obtain solution a; In solution a, add matrine, fully stir, it is mixed, obtain bactericide stoste b; In bactericide stoste b, add bleeding agent, obtain bactericide of the present invention.
Embodiment 2
Ratio in matrine 0.01wt%, humus 20wt%, bleeding agent 0.1wt% and deionized water 79.89wt% is chosen matrine, humus, bleeding agent and deionized water; In reactor, add deionized water, humus is added by proportioning, fully stir, it is dissolved fully, obtain solution a; In solution a, add matrine, fully stir, it is mixed, obtain bactericide stoste b; In bactericide stoste b, add bleeding agent, obtain bactericide of the present invention.
Embodiment 3
Ratio in matrine 5.0wt%, humus 0.1wt%, bleeding agent 5.0wt% and deionized water 89.9wt% is chosen matrine, humus, bleeding agent and deionized water; In reactor, add deionized water, humus is added by proportioning, fully stir, it is dissolved fully, obtain solution a; In solution a, add matrine, fully stir, it is mixed, obtain bactericide stoste b; In bactericide stoste b, add bleeding agent, obtain bactericide of the present invention.
The test example
The bactericide that embodiment 1-3 is made adds respectively in the sterilization conical flask, adds an amount of methyl alcohol again it is fully dissolved, and adds the sterile water dilution then and is settled to 50ml, and each 50ml of test mother liquor that makes concentration and be 48000 μ g/g is standby.
On the PDA medium, with apple rot pathogen continuous culture several generations, preceding 3 days then in test, again switching once, 28 ℃ to cultivate mycelia down long to a certain degree, standby;
Under aseptic technique, draw the 2ml mother liquor respectively, add respectively in the aseptic conical flask, the sterilising medium that will melt is in advance measured 18ml and is added in the above-mentioned aseptic conical flask again, fully shakes up.Pour into immediately then in the culture dish that diameter is 9cm, make the pastille flat board (this moment, the mother liquor dilution was 10 times) of respective concentration; Other establish do not contain soup processing as blank, repeat 4 times.
With cultured pathogen, under aseptic condition, be the card punch of 5mm with diameter, cut the bacterium cake from colony edge, with transfer needle the bacterium cake is inoculated in the dull and stereotyped central authorities of pastille, the mycelia face covers the ware lid by medium, puts in 28 ℃ of insulating boxs and cultivates 72 hours.
Adopting vernier scale measurement colony diameter, unit is millimeter (mm), and after cultivating 72 hours for the examination bacterium colony, each bacterium colony employing right-angled intersection method vertical survey diameter is once got its mean value.
Calculate bactericide to the mycelial growth inhibition rate for test-object target bacterium, unit is %, and result of calculation keeps 2 significant digits, and computing formula is: I=(D
0-D
t)/D
0* 100%, wherein I is that bactericide is to the mycelial growth inhibition rate for test-object target bacterium; D
0For the blank bacterium colony increases diameter; D
tHandle bacterium colony for soup and increase diameter.
When carrying out the medicament joint toxicity measuring, adopt the Wadley method to calculate the coefficient of synergism (SR) of mixture, estimate mixture associating result of use.
Test result is as shown in the table:
Result of the test shows: it is obvious that bactericide of the present invention suppresses effect to apple rot pathogen, and it is obvious that humus and matrine mix the back synergistic effect.
Above listed each embodiment enumerates of the present invention, and those skilled in the art can understand the simple modification done, replacement or belong to protection scope of the present invention with combining of this area customary means on basis of the present invention.
Claims (3)
1. composition fungicide that contains matrine and humic acid, its raw material comprises: 0.01-5.0wt% matrine, 0.1-20.0wt% humus, 0.1-5.0wt% bleeding agent and balance of deionized water.
2. bactericide according to claim 1, wherein the content of each raw material is: matrine 0.3wt%, humus 2.7wt%, bleeding agent 2.0wt% and balance of deionized water.
3. method for preparing the described bactericide of claim 1, it may further comprise the steps:
(1) chooses matrine, humus, bleeding agent and deionized water in proportion;
(2) in reactor, add deionized water, humus is added by proportioning, fully stir, it is dissolved fully, obtain solution a;
(3) in solution a, add matrine, fully stir, it is mixed, obtain bactericide stoste b;
(4) in bactericide stoste b, add bleeding agent, obtain bactericide of the present invention.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102545930A CN102283252A (en) | 2011-08-31 | 2011-08-31 | Composition disinfectant containing matrine and humic acid and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102545930A CN102283252A (en) | 2011-08-31 | 2011-08-31 | Composition disinfectant containing matrine and humic acid and preparation method thereof |
Publications (1)
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| CN102283252A true CN102283252A (en) | 2011-12-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN2011102545930A Pending CN102283252A (en) | 2011-08-31 | 2011-08-31 | Composition disinfectant containing matrine and humic acid and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102674945A (en) * | 2011-12-31 | 2012-09-19 | 乌鲁木齐木易联华生物科技有限公司 | Organic biological pesticide fertilizer |
| CN102838414A (en) * | 2012-09-15 | 2012-12-26 | 山东农业大学 | Pesticide for preventing and controlling northern fruit tree root rot and application of pesticide |
| CN104886175A (en) * | 2015-06-01 | 2015-09-09 | 山东省农业科学院农业质量标准与检测技术研究所 | Pesticide for preventing and treating rotten disease of apple trees |
| CN106386857A (en) * | 2016-08-31 | 2017-02-15 | 王山辉 | Preparation for controlling rot disease of fruit trees and method thereof |
| CN108276215A (en) * | 2018-02-13 | 2018-07-13 | 广西中烟工业有限责任公司 | A kind of tobacco seedlings dedicated fertilizer and preparation method thereof with rooting promoting |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1280775A (en) * | 2000-08-15 | 2001-01-24 | 西北农业大学无公害农药研究服务中心 | Sophora herb disinfectant and its preparing process |
| CN1847200A (en) * | 2006-04-26 | 2006-10-18 | 张洪昌 | Pollution-less pesticide fertilizer for preventing and controlling blight and its production process |
| CN101233867A (en) * | 2008-02-26 | 2008-08-06 | 天津市恒源伟业生物科技发展有限公司 | Matrine agent plant pesticide and preparation thereof |
-
2011
- 2011-08-31 CN CN2011102545930A patent/CN102283252A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1280775A (en) * | 2000-08-15 | 2001-01-24 | 西北农业大学无公害农药研究服务中心 | Sophora herb disinfectant and its preparing process |
| CN1847200A (en) * | 2006-04-26 | 2006-10-18 | 张洪昌 | Pollution-less pesticide fertilizer for preventing and controlling blight and its production process |
| CN101233867A (en) * | 2008-02-26 | 2008-08-06 | 天津市恒源伟业生物科技发展有限公司 | Matrine agent plant pesticide and preparation thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102674945A (en) * | 2011-12-31 | 2012-09-19 | 乌鲁木齐木易联华生物科技有限公司 | Organic biological pesticide fertilizer |
| CN102674945B (en) * | 2011-12-31 | 2014-12-24 | 乌鲁木齐木易联华生物科技有限公司 | Organic biological pesticide fertilizer |
| CN102838414A (en) * | 2012-09-15 | 2012-12-26 | 山东农业大学 | Pesticide for preventing and controlling northern fruit tree root rot and application of pesticide |
| CN102838414B (en) * | 2012-09-15 | 2014-02-26 | 山东农业大学 | Pesticide for preventing and controlling northern fruit tree root rot and application of pesticide |
| CN104886175A (en) * | 2015-06-01 | 2015-09-09 | 山东省农业科学院农业质量标准与检测技术研究所 | Pesticide for preventing and treating rotten disease of apple trees |
| CN106386857A (en) * | 2016-08-31 | 2017-02-15 | 王山辉 | Preparation for controlling rot disease of fruit trees and method thereof |
| CN108276215A (en) * | 2018-02-13 | 2018-07-13 | 广西中烟工业有限责任公司 | A kind of tobacco seedlings dedicated fertilizer and preparation method thereof with rooting promoting |
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Application publication date: 20111221 |