CN104304325A - Growing ginger treating agent for preventing ginger blast - Google Patents
Growing ginger treating agent for preventing ginger blast Download PDFInfo
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- CN104304325A CN104304325A CN201410594132.1A CN201410594132A CN104304325A CN 104304325 A CN104304325 A CN 104304325A CN 201410594132 A CN201410594132 A CN 201410594132A CN 104304325 A CN104304325 A CN 104304325A
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Abstract
The invention relates to a growing ginger treating agent for preventing ginger blast. The ginger treating agent comprises active components, resistance inducer and auxiliaries, wherein the active components are compound compositions of pyrimidine nucleoside antibiotic and benziothiazolinone; effective components used for the treating agent are low-toxic medicaments, are safe to non-target organisms and environment, free of bad harm to human body and suitable for treating fresh growing gingers; particularly, compared with single dosage, because of compounding of the medicaments, the synergism is realized; meanwhile, the difference of microbe-killing mechanisms of two different medicaments is utilized, the production of drug resistance of the ginger blast is relieved.
Description
Technical field
The invention belongs to technical field of pesticide, particularly relate to a kind ginger inorganic agent, more particularly relating to a kind of for preventing the kind ginger inorganic agent of bacterial wilt of ginger.
Technical background
Ginger is one of pungent health-care vegetable of main perfume (or spice) of extensive use all over the world, is also the important source material of food, medicine and chemical products.Add up according to FAO (Food and Agriculture Organization of the United Nation) FAO, about there are more than 50 country's plantation gingers in the current whole world, and importer then reaches more than 150.China is ginger cultivation big country, and Ginger Yield accounts for Gross World Product 38%.
Bacterial wilt of ginger is the important disease hindering the sustainable development of Chinese ginger industry, and occur serious, difficulty of prevention and cure is large.Research is thought, bacterial wilt of ginger is the crushing soil-borne disease of one caused by the withered Pseudomonas alba of green grass or young crops (Ralstonia solanacearum).The long-term Field diseases in main Zai Jiang district of China is generally 10% ~ 20%, and severe patient reaches 30% ~ 40%, and local field even has no harvest.
The major measure of current control bacterial wilt of ginger is soil treatment, plants ginger Medicament soaked seed and later stage medicament root irrigation.Soil treatment effect is better, but processing soil treatment agent toxicity is high, serious to ecological disruption, has high input; Plant the process of ginger sterilization many employings Medicament soaked seed, the method lasting period is short, and preventive effect is poor; Later stage chemical agent root irrigation, medicament usage amount is large, to environment and ginger safety effects huge.Therefore, develop the new medicament of high-efficiency low-toxicity and use new prevention and controls prevention bacterial wilt of ginger extremely urgent.
Plant ginger inorganic agent to be processed into by compositions such as bactericide, inducer, film forming agent, thickener, emulsifying dispersant, antifreezing agent, fillers; the surface of the seed can be coated on after dilution; form the diaphragm with some strength and water-permeable and air permeable, slow releasing effective ingredient.After this formulation process kind ginger, form medicament protective layer, hinder the intrusion of germ by its film forming on kind of a ginger surface, again by its slow release, improving service efficiency and the lasting period of medicament, is the effective way of alternative traditional Medicament soaked seed.
Summary of the invention
The object of this invention is to provide a kind of for kind of a reagent combination for ginger process, bacterial wilt of ginger can be prevented efficiently.
Based on this purpose, technical scheme of the present invention is:
A kind of kind ginger inorganic agent preventing bacterial wilt of ginger, it is characterized in that, described kind of ginger inorganic agent comprises active component, inducer and auxiliary agent, wherein the consumption of active component is 0.5 ~ 10wt%, active component is the complex composition of antibiotic in pyrimidine nucleoside class and benziothiazolinone, and the weight ratio of the two is 4:1 ~ 1:4; The consumption of inducer is 0.001-0.1wt%; Auxiliary agent is wetting agent 0.1 ~ 2wt%, emulsifying dispersant 1 ~ 6wt%, film forming agent 1 ~ 10wt%, antifreezing agent 0.5 ~ 3wt%, defoamer 0.2 ~ 2wt%, and thickener 0.1 ~ 2wt%, filler 10 ~ 40wt%, all the other supply 100% by water.
Described inducer is one or more the combination in poly-GAG, salicylic acid, probenazole, Benzothiadiazole, methyl jasmonic acid.
Described wetting agent is one or more the combination in neopelex, dioctyl sodium sulfosuccinate and nekal.
Described emulsifying dispersant is one or more the combination in sorbitol polyoxyethylene ether, sodium lignin sulfonate, polyarylphenol-polyoxyethylene ether phosphate, alkylphenol polyoxyethylene.
Described film forming agent is one or more combination of polyvinyl alcohol (PVA), ethene and acetate ethylene copolymer (VAE), polyacrylate, polyvinyl acetate, shitosan and benzene emulsion.
Described antifreezing agent is ethylene glycol.
Described defoamer is n-octyl alcohol.
Described thickener is one in xanthan gum, sodium alginate, guar gum and sodium carboxymethylcellulose or mixture.
Described filler is one or more mixtures of bentonite, diatomite and attapulgite.
Preferably, the consumption of active component is 1 ~ 5wt%; More preferably, the consumption of active component is 2wt%;
Preferably, the weight ratio 3:1 ~ 1:3 of active component antibiotic in pyrimidine nucleoside class and benziothiazolinone, more preferably, the weight ratio of the two is 1:3.
The invention still further relates to the purposes being used for above-mentioned kind of ginger inorganic agent to prevent bacterial wilt of ginger.
Except as otherwise noted, " % " in this specification is " percentage by weight ".
The preparation method of of the present invention kind of ginger inorganic agent is as follows: first take each constituent in proportion, joined by film forming agent in the water of 50%, add thermal agitation, make it dissolve completely, for subsequent use; Subsequently by after other constituent and residue 50% water Homogeneous phase mixing, put into colloid mill and carry out preliminary ground and mixed 20 minutes together with film forming agent solution, re-use multi-functional grinding agitator grinding 30min, then put into emulsify at a high speed clipper and shear 10 minutes with 7000-10000r/min speed high-speed stirred, of the present invention kind of ginger inorganic agent can be obtained.
The present invention plants the using method of ginger inorganic agent: kind of a ginger inorganic agent is diluted 40 times, the kind ginger cleaned is immersed in dilution, soaks and pull out after 1-3 hour, sow after drying behind cool place.
Of the present invention kind of ginger inorganic agent active ingredient used is low-toxic agent, to non-target organism and Environmental security, to human body without bad harm, is applicable to the process of ginger kind ginger.
Of the present invention kind of ginger inorganic agent active ingredient focuses on the composite use of medicament, and the therapeutic action of agricultural chemicals and protective effect are combined, compare single dose, the composite use of described medicament reaches synergistic effect; Further, also utilize the difference of the sterilization mechanism of two kinds of different agents, alleviate the drug-fast generation of ginger plague germ.
Seed soaking kind of ginger after of the present invention kind of ginger inorganic agent dilution certain multiple; kind of a ginger surface is made to form the diaphragm with some strength and water-permeable and air permeable; meet after entering in soil and water-solublely rise but do not dissolve, slow releasing effective ingredient, reach the object of long-time prevention bacterial wilt of ginger.Compared with soaking kind of ginger with traditional medicament, overcome its term of validity short, not there is the shortcomings such as slow release.Meanwhile, inducer add the resistance that can improve ginger self, improve further the effect of prevention bacterial wilt of ginger.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, the following instantiation of the present invention is described, but the present invention is not limited to these specific embodiments.
Preparation embodiment
Embodiment 1:
Antibiotic in pyrimidine nucleoside class 0.4wt%; Benziothiazolinone 1.6wt%; Benzothiadiazole 0.05wt%; PVA 6wt%; VAE 2wt%; Xanthans 0.2wt%, alkylphenol polyoxyethylene 2wt%, nekal 1.5wt%, ethylene glycol 2%; N-octyl alcohol 2wt%, bentonite 15wt%, surplus water is supplied.
Embodiment 2:
Antibiotic in pyrimidine nucleoside class 0.5wt%; Benziothiazolinone 1.5wt%; Benzothiadiazole 0.05wt%; PVA 6wt%; VAE 2wt%; Xanthans 0.2wt%, alkylphenol polyoxyethylene 2wt%, nekal 1.5wt%, ethylene glycol 2%; N-octyl alcohol 2wt%, bentonite 15wt%, surplus water is supplied.
Embodiment 3:
Antibiotic in pyrimidine nucleoside class 0.7wt%; Benziothiazolinone 1.3wt%; Benzothiadiazole 0.05wt%; PVA 6wt%; VAE 2wt%; Xanthans 0.2wt%, alkylphenol polyoxyethylene 2wt%, nekal 1.5wt%, ethylene glycol 2%; N-octyl alcohol 2wt%, bentonite 15wt%, surplus water is supplied.
Embodiment 4:
Antibiotic in pyrimidine nucleoside class 1wt%; Benziothiazolinone 1wt%; Benzothiadiazole 0.05wt%; PVA 6wt%; VAE 2wt%; Xanthans 0.2wt%, alkylphenol polyoxyethylene 2wt%, nekal 1.5wt%, ethylene glycol 2%; N-octyl alcohol 2wt%, bentonite 15wt%, surplus water is supplied.
Embodiment 5:
Antibiotic in pyrimidine nucleoside class 1.3wt%; Benziothiazolinone 0.7wt%; Benzothiadiazole 0.05wt%; PVA 6wt%; VAE 2wt%; Xanthans 0.2wt%, alkylphenol polyoxyethylene 2wt%, nekal 1.5wt%, ethylene glycol 2%; N-octyl alcohol 2wt%, bentonite 15wt%, surplus water is supplied.
Embodiment 6:
Antibiotic in pyrimidine nucleoside class 1.5wt%; Benziothiazolinone 0.5wt%; Benzothiadiazole 0.05wt%; PVA 6wt%; VAE 2wt%; Xanthans 0.2wt%, alkylphenol polyoxyethylene 2wt%, nekal 1.5wt%, ethylene glycol 2%; N-octyl alcohol 2wt%, bentonite 15wt%, surplus water is supplied.
Embodiment 7:
Antibiotic in pyrimidine nucleoside class 1.6wt%; Benziothiazolinone 0.4wt%; Benzothiadiazole 0.05wt%; PVA 6wt%; VAE 2wt%; Xanthans 0.2wt%, alkylphenol polyoxyethylene 2wt%, nekal 1.5wt%, ethylene glycol 2%; N-octyl alcohol 2wt%, bentonite 15wt%, surplus water is supplied.
Embodiment 8:
Antibiotic in pyrimidine nucleoside class 0.25wt%; Benziothiazolinone 0.75wt%; Benzothiadiazole 0.05wt%; PVA 6wt%; VAE 2wt%; Xanthans 0.2wt%, alkylphenol polyoxyethylene 2wt%, nekal 1.5wt%, ethylene glycol 2%; N-octyl alcohol 2wt%, bentonite 15wt%, surplus water is supplied.
Embodiment 9:
Antibiotic in pyrimidine nucleoside class 0.125wt%; Benziothiazolinone 0.375wt%; Benzothiadiazole 0.05wt%; PVA 6wt%; VAE 2wt%; Xanthans 0.2wt%, alkylphenol polyoxyethylene 2wt%, nekal 1.5wt%, ethylene glycol 2%; N-octyl alcohol 2wt%, bentonite 15wt%, surplus water is supplied.
Embodiment 10:
Antibiotic in pyrimidine nucleoside class 1wt%; Benziothiazolinone 3wt%; Benzothiadiazole 0.05wt%; PVA 6wt%; VAE 2wt%; Xanthans 0.2wt%, alkylphenol polyoxyethylene 2wt%, nekal 1.5wt%, ethylene glycol 2%; N-octyl alcohol 2wt%, bentonite 15wt%, surplus water is supplied.
Embodiment 11:
Antibiotic in pyrimidine nucleoside class 2wt%; Benziothiazolinone 6wt%; Benzothiadiazole 0.05wt%; PVA 6wt%; VAE 2wt%; Xanthans 0.2wt%, alkylphenol polyoxyethylene 2wt%, nekal 1.5wt%, ethylene glycol 2%; N-octyl alcohol 2wt%, bentonite 15wt%, surplus water is supplied.
Biological Examples
1. indoor bactericidal activity measures
1.1 Study on Fungicide Toxicity
Adopt Odontothrips loti mensuration antibiotic in pyrimidine nucleoside class and benziothiazolinone to the inhibit activities of ginger plague germ.2 kinds of former medicines are configured to 5 suitable concentrations respectively, get 0.2ml respectively and add in the cup of Oxford, after 24h, be determined at the inhibition zone size of different disposal, calculate colony growth inhibiting rate (%) by inhibition zone.According to growth inhibition ratio measuring and calculating medicament virulence.With the logarithm value of drug concentration (mg/kg) for independent variable, the probability value of antibacterial growth rate is dependent variable, sets up virulence regression equation, obtains the EC of 2 kinds of medicaments
50.
1.2.2 compound drug is selected and optimum proportioning screening
Undertaken composite by antibiotic in pyrimidine nucleoside class and benziothiazolinone: two kinds of former medicines are mixed with 4:1 by active ingredient, 3:1,2:1,1:1,1:2,1:3,1:4 be totally 7 proportionings (mass ratio).Above-mentioned formula carries out toxicity test by Study on Fungicide Toxicity method respectively.
Mixture synergy is evaluated, with EC
50based on value, evaluate mixture synergy type by calculating mixture co-toxicity coefficient.
The A medicament chosen wherein is standard agent, and each medicament toxicity index calculates as follows:
Theoretical toxicity index (the TTI)=P of mixture
a× A+P
b× B
Wherein P
a, P
bbe respectively the percentage composition of two kinds of former medicines in mixture, A, B are respectively the toxicity index of two kinds of former medicines.
CTC >=170 are notable synergistic, and 120≤CTC<170 is slightly synergy, and 70≤CTC<120 is summation action, and CTC<70 is antagonism.
2. result of the test
2.1 Study on Fungicide Toxicity results
Table 12 kinds of pesticide original medicines are to the Toxicity Determination result of ginger plague germ
As can be seen from Table 1, antibiotic in pyrimidine nucleoside class is to the EC of ginger plague germ
50be 3616.30 μ g/mL; Benziothiazolinone is to the EC of ginger plague germ
50be 1774.95 μ g/mL.
Table 2 antibiotic in pyrimidine nucleoside class and the composite Toxicity Determination result to ginger plague germ of benziothiazolinone
As shown in Table 2, the composite all proportions of antibiotic in pyrimidine nucleoside class and benziothiazolinone all shows certain synergistic effect, but different compound proportion coefficient of synergism difference to some extent.Wherein, when the compound proportion of antibiotic in pyrimidine nucleoside class and benziothiazolinone is 1:3, co-toxicity coefficient is the highest, is 166.49.
Two, field experiment
1. test method
Get kind of a ginger inorganic agent and dilute 40 times of process ginger kinds, test often processes and repeats for 4 times, establishes 2 row isolation strips between treatment region group, and adopting agricultural streptomycin to dilute 100 times of immersion kinds is medicament contrast, with water logging kind for clear water contrasts.Then carry out field experiment according to the method for randomized block experiment, measure germination rate and the field efficacy of field experiment, and finally determine bacterial wilt of ginger kind ginger inorganic agent treatment formulations according to field experiment.
To emerge " Invest, Then Investigate " emergence rate at ginger, respectively at bacterial wilt of ginger their early stage, morbidity mid-term and morbidity later stage investigation bacterial wilt of ginger incidence.The investigation of every community diagonal 5 fixed point, often some sampling survey 20 nest, adopts classification counting method, adds up according to the sick pier number of severity Scaling standard to each community.
Stage division:
0 grade: anosis;
1 grade: maternal plant partial onset, the strain of descendants ginger is anosis;
3 grades: descendants Jiang Zhu has scab but without withered;
5 grades: Jiang Cong local withered (30%-50%);
7 grades: Jiang Cong is substantially withered or entirely withered, and ginger meat 60% variable color is rotted;
9 grades: Jiang Cong is completely withered, and ginger meat 90% variable color is rotted.
Drug effect computational methods
In formula: CK---blank district disease index;
PT---chemicals treatment district disease index;
2. result of the test
As can be seen from Table 3.
The impact that table 3 different formulations seed coat agent is emerged on kind of ginger and field control effect
A kind of seed coat agent of bacterial wilt of ginger of preventing of the present invention is described by specific embodiment.Those skilled in the art can use for reference the links such as the suitable feed change of content of the present invention, process conditions and realize other object corresponding, its relevant change does not all depart from content of the present invention, all similar replacements and change will become apparent to those skilled in the art that and be all deemed to be included within scope of the present invention.
Claims (9)
1. one kind is prevented the kind ginger inorganic agent of bacterial wilt of ginger, it is characterized in that, described kind of ginger inorganic agent comprises active component, inducer and auxiliary agent, wherein the consumption of active component is 0.5 ~ 10wt%, active component is the complex composition of antibiotic in pyrimidine nucleoside class and benziothiazolinone, and the weight ratio of the two is 4:1 ~ 1:4; The consumption of inducer is 0.001-0.1wt%; Auxiliary agent is wetting agent 0.1 ~ 2wt%, emulsifying dispersant 1 ~ 6wt%, film forming agent 1 ~ 10wt%, antifreezing agent 0.5 ~ 3wt%, defoamer 0.2 ~ 2wt%, and thickener 0.1 ~ 2wt%, filler 10 ~ 40wt%, all the other supply 100% by water.
2. according to claim a kind of ginger inorganic agent, is characterized in that, described inducer is one or more the combination in poly-GAG, salicylic acid, probenazole, Benzothiadiazole, methyl jasmonic acid; Described wetting agent is one or more the combination in neopelex, dioctyl sodium sulfosuccinate and nekal; Described emulsifying dispersant is one or more the combination in sorbitol polyoxyethylene ether, sodium lignin sulfonate, polyarylphenol-polyoxyethylene ether phosphate, alkylphenol polyoxyethylene; Described film forming agent is one or more combination of polyvinyl alcohol (PVA), ethene and acetate ethylene copolymer (VAE), polyacrylate, polyvinyl acetate, shitosan and benzene emulsion; Described antifreezing agent is ethylene glycol; Described defoamer is n-octyl alcohol; Described thickener is one in xanthan gum, sodium alginate, guar gum and sodium carboxymethylcellulose or mixture; Described filler is one or more mixtures of bentonite, diatomite and attapulgite.
3. according to claim 1 and 2 kind of ginger inorganic agent, is characterized in that, the consumption of active component is 1 ~ 5wt%.
4. according to claim 3 kind of ginger inorganic agent, is characterized in that, the consumption of active component is 2wt%.
5. according to claim 1 and 2 kind of ginger inorganic agent, is characterized in that, the weight ratio of active component antibiotic in pyrimidine nucleoside class and benziothiazolinone is 3:1 ~ 1:3.
6. according to claim 5 kind of ginger inorganic agent, is characterized in that, the weight ratio of active component antibiotic in pyrimidine nucleoside class and benziothiazolinone is 1:3.
7. according to claim 1 and 2 kind of ginger inorganic agent is for preventing the purposes of bacterial wilt of ginger.
8. the preparation method of according to claim 1 and 2 kind of ginger inorganic agent, is characterized in that, first takes each constituent in proportion, is joined by film forming agent in the water of 50%, add thermal agitation, make it dissolve completely, for subsequent use; Subsequently by after other constituent and residue 50% water Homogeneous phase mixing, put into colloid mill and carry out preliminary ground and mixed 20 minutes together with film forming agent solution, re-use multi-functional grinding agitator grinding 30min, then put into emulsify at a high speed clipper and shear 10 minutes with 7000-10000r/min speed high-speed stirred, obtained kind of ginger inorganic agent.
9. the using method of according to claim 1 and 2 kind of ginger inorganic agent, is characterized in that, kind of a ginger inorganic agent is diluted 40 times, the kind ginger cleaned is immersed in dilution, soaks and pull out after 1-3 hour, sow after drying behind cool place.
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Cited By (4)
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| CN105123784A (en) * | 2015-09-18 | 2015-12-09 | 昆明线敌生物科技有限公司 | Traditional Chinese medicine preparation for preventing and treating ginger bacterial wilt and preparation method of traditional Chinese medicine preparation |
| CN107333816A (en) * | 2017-07-31 | 2017-11-10 | 贞丰县连环生姜农民专业合作社 | A kind of medicament for treating bacterial wilt of ginger and preparation method thereof |
| CN109287641A (en) * | 2018-10-30 | 2019-02-01 | 江苏辉丰生物农业股份有限公司 | A kind of microbicide compositions |
| CN111955096A (en) * | 2020-08-05 | 2020-11-20 | 重庆文理学院 | Medicament-compounded seed ginger coating method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105123784A (en) * | 2015-09-18 | 2015-12-09 | 昆明线敌生物科技有限公司 | Traditional Chinese medicine preparation for preventing and treating ginger bacterial wilt and preparation method of traditional Chinese medicine preparation |
| CN107333816A (en) * | 2017-07-31 | 2017-11-10 | 贞丰县连环生姜农民专业合作社 | A kind of medicament for treating bacterial wilt of ginger and preparation method thereof |
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| CN111955096A (en) * | 2020-08-05 | 2020-11-20 | 重庆文理学院 | Medicament-compounded seed ginger coating method |
| CN111955096B (en) * | 2020-08-05 | 2022-07-29 | 重庆文理学院 | Seed ginger coating method by compounding medicament |
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