CN101530096B - Sterilizing composition compounded by metalaxyl and teasaponin and application thereof - Google Patents
Sterilizing composition compounded by metalaxyl and teasaponin and application thereof Download PDFInfo
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- CN101530096B CN101530096B CN2009100383440A CN200910038344A CN101530096B CN 101530096 B CN101530096 B CN 101530096B CN 2009100383440 A CN2009100383440 A CN 2009100383440A CN 200910038344 A CN200910038344 A CN 200910038344A CN 101530096 B CN101530096 B CN 101530096B
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Abstract
The invention discloses a sterilizing composition compounded by metalaxyl and teasaponin and application thereof. The sterilizing composition comprises the following components in percentage by weight: 0.5 to 80 percent of metalaxyl, 0.01 to 80 percent of teasaponin and the balance of additives. The sterilizing composition can be prepared into missible oil, wettable powder, microemulsion, suspending agents, suspending emulsion, aqueous emulsion or soluble liquid. The sterilizing composition adopts the metalaxyl and the teasaponin to compound, has obvious synergized actions, obviously improves the bactericidal activity compared with a single dosage, and has the effect of treating various diseases by using one medicament so as to reduce the input of labor and resources in the process of controlling the diseases, and improve the productivity effect. At the same time, on the premise of guaranteeing the drug effect, the cost is obviously reduced. The sterilizing composition has the advantages of various dosage forms, simple preparation method and high popularizing value.
Description
Technical field
The invention belongs to agricultural bactericidal preparation technique field, be specifically related to composite bactericidal composition of a kind of metalaxyl and Tea Saponin and application thereof.
Background technology
Because long-term, a large amount of unreasonable uses of chemical pesticide, and because the intrinsic shortcoming of some medicament itself has caused serious pesticide resistance problem.It is good to seek Environmental compatibility, and to the public nuisance-free agricultural chemicals that pathogen has lasting effect, important seemed in today that pollution-free food is much accounted of day by day.
Natural products often shows many biologically actives, and widely people are used for hundreds of years.Wherein, the research and development of plant resource fungicide active ingredient is a research direction that solves the many negative effects of current chemical bactericide.Therefore, be object with the natural products, seek bioactivator, the research and development novel biopesticide enjoys attention at home and abroad, and wherein, botanical pesticide is the most noticeable one type.Being mixed of the exploitation of botanical pesticide, botanical pesticide and chemical pesticide, botanical pesticide and being mixed of botanical pesticide all are the forward position and the focus of pesticide research in the world.
Metalaxyl is to belong to the hypotoxicity systemic fungicide, has protection and therapeutic action, and two-way conductive performance is arranged.To Pseudoperonospora cubensis, phytophthora root rot bacterium and rotten germ various crop downy mildew, phytophthora root rot, the millet downy mildew of gourd, fruit and vegetable class are effective.Single this medicine of long-term use, germ are prone to produce resistance.
Tea Saponin (Tea saponin) is the bactericide that from the tea cake after the seed of plant of theaceae such as tea seed cake, the tea-seed oil expression, extracts, and belongs to pentacyclic triterpenoid, and its pure article are colorless prismatic crystal, have hygroscopicity, and bitter is pungent, and molecular formula is C
57H
90O
26, molecular weight is 1191.31,223～224 ℃ of fusing points, and soluble in water and organic solvents such as alcohols, organic acid are insoluble to organic solvents such as ether, benzinum, benzene.
Tea Saponin is a kind of nonionic surface active agent, has that stronger exhibition is puted forth effort, emulsifying ability, dispersion force, wetting power and a foaming power, and cold-blooded animal is had high poison, and people and animals are waited the nontoxic or low toxicity of homoiothermy animal.This compound has sterilization, sterilization, kills mite and reduces capillary multiple function, has different mechanism of action with chemical pesticide, and through improve soup wetting, opening up, emulsifying dispersivity has significant synergies to chemical pesticide.Tea Saponin is typical no-pollution multifunctional plant pesticide kind, and for example at Chinese countryside, the tea bran always is exactly a soil pesticide, can kill oncomelania as pool-cleaning agent, makes bactericide with immersion tea bran water and can prevent rice blast and banded sclerotial blight.After deliberation, Tea Saponin itself has good inhibition effect to the various plants growth of pathogenic bacteria, is a kind of typical multi-functional plant source pesticide kind.But this medicine quick-acting is poor, and application dosage is very big when using separately.
Summary of the invention
The objective of the invention is to make full use of the unique effect mechanism of rich resources of tea saponin and its botanical pesticide, confirm itself and the suitable compatibility of metalaxyl, provide a kind of efficiently, wide spectrum, be difficult for developing immunity to drugs, the bactericidal composition of low toxicity.
Another object of the present invention provides the application of said bactericidal composition.
The object of the invention is achieved through following technical scheme:
Provide a kind of metalaxyl and Tea Saponin composite bactericidal composition, comprise following components in weight percentage:
Metalaxyl: 0.5～80%;
Tea Saponin: 0.01～80%;
The auxiliary agent surplus.
Said bactericide is missible oil, wetting powder, microemulsion, suspending agent, suspended emulsion, aqueous emulsion or soluble liquid, and the preparation method who is adopted is the method that adopts usually during corresponding formulation is produced.
Described auxiliary agent is different and different according to formulation, is exemplified below:
When preparation missible oil, its auxiliary agent is:
Surfactant comprise preparation by weight 5～30%; Surfactant is the polyethenoxy ether class of nonionic; Or the sodium alkyl sulfate of anionic or in the alkyl benzene sulfonate one or more, use when multiple, total usage amount can not greater than deal maximum;
Solvent accounts for preparation by weight 10～80%; Solvent is various aromatic hydrocarbons, aliphatic hydrocarbon, ketone, ethers; In available benzene,toluene,xylene, naphthalane, gasoline, kerosene, machine oil, MEK, propylene glycol, butyl glycol ether, the benzinum one or more; Use when multiple, total usage amount can not greater than deal maximum;
Cosolvent accounts for preparation by weight 0.5～20%, and cosolvent is one or more in methyl alcohol, isoamyl alcohol, phenol, ethyl acetate, the dimethyl sulfoxide (DMSO), use when multiple, total usage amount can not greater than deal maximum;
PH value conditioning agent accounts for preparation by weight 0.5～1%, and pH value conditioning agent is one or more in sodium hydroxide, ammonium hydroxide, acetic acid, the hydrochloric acid, use when multiple, total usage amount can not greater than deal maximum;
Synergist accounts for preparation by weight 0.5～25%, and synergist is one or more in Butacide, sulfoxide, alkyl carbonate, chlorination fat, the formaldehyde, use when multiple, total usage amount can not greater than deal maximum;
Anti-photodissociation agent accounts for preparation by weight 0.5～15%, and anti-photodissociation agent is one or more in p-aminobenzoic acid, castor oil, the Angelica oil, use when multiple, total usage amount can not greater than deal maximum;
Stabilizer comprises preparation by weight 0.5～15%, stabilizing agent is one or more in epoxidized soybean oil, the epoxidized linseed, use when multiple, total usage amount can not greater than deal maximum;
Penetrating agent accounts for preparation by weight 0.5～2%, and penetrating agent is one or more in Laurocapram, JFC-2, the maleic acid di-sec-octyl sodium sulfonate, use when multiple, total usage amount can not greater than deal maximum;
Or when the preparation wetting powder, its auxiliary agent is:
Wetting agent accounts for preparation by weight 2.5～35%; Wetting agent is natural products wetting agent and anionic wetting agent; Available tea is withered, spaonin powder, soapberry powder, silkworm excrement, bay alcohol radical sodium sulphate, neopelex, washing powder, draw back in the powder one or more; Use when multiple, total usage amount can not greater than deal maximum;
Dispersant accounts for preparation by weight 2.5～30%; Dispersant is industrial by-products and anionic dispersing agent; In available lignosulfite, lignin and derivative sulfonate, naphthalene and alkylbenzene formaldehyde condensation products sulfonate, the alkylsulfonate one or more; Use when multiple, total usage amount can not greater than deal maximum;
Filler accounts for preparation by weight 10～70%, and filler is mineral type carrier or synthetic vectors, and one or more in available kaolin, diatomite, the white carbon use when multiple, total usage amount can not greater than deal maximum;
Or when the preparation microemulsion, its auxiliary agent is:
Solvent accounts for preparation by weight 10～80%, and solvent is one or more in alcohols, ketone, ester class and the aromatic hydrocarbon, use when multiple, total usage amount can not greater than deal maximum;
Emulsifier accounts for preparation by weight 5～30%; Emulsifier is alkyl benzene sulfonate calcium salt, alkyl benzene sulphonate magnesium salts, alkyl benzene sulphonate aluminium salt, alkyl benzene sulphonate sodium salt, alkyl benzene sulphonate barium salt, C8～C20 alkylsurfuric acid sodium salt, styrene polyoxyethylene ether ammonium sulfate salt, benzylbiphenyl phenol polyethenoxy ether, phenethyl phenol polyethenoxy (N=15～30) ether, phenethyl phenol polyethenoxy polyoxypropylene, NPE, APES first condensation product, xenol APEO, homemade Nongru-700 number, in No. 300 one or more; Use when multiple, total usage amount can not greater than deal maximum;
Stabilizer comprises preparation by weight 0.5～15%; Stabilizing agent is a 3-chloro-1; In 2-expoxy propane, butyl glycidyl ether, phenyl glycidyl ether, cresyl glycidyl ether, polyvinyl one glycol diglycidyl ether, the sodium sorbate one or more; Use when multiple, total usage amount can not greater than deal maximum;
Antifreezing agent accounts for preparation by weight 0.5～10%, and antifreezing agent is one or more in ethylene glycol, glycerine, propane diols, polyethylene glycol, the sorb sodium alkoxide, use when multiple, total usage amount can not greater than deal maximum;
Or when the preparation suspending agent, its auxiliary agent is:
Surfactant comprise preparation by weight 5～30%; Surfactant is the polyethenoxy ether class of nonionic; Or the sodium alkyl sulfate of anionic or in the alkyl benzene sulfonate one or more, use when multiple, total usage amount can not greater than deal maximum;
Solvent accounts for preparation by weight 10～80%; Solvent is various aromatic hydrocarbons, aliphatic hydrocarbon, ketone, ethers; In available benzene,toluene,xylene, naphthalane, gasoline, kerosene, machine oil, MEK, propylene glycol, butyl glycol ether, the benzinum one or more; Use when multiple, total usage amount can not greater than deal maximum;
Cosolvent accounts for preparation by weight 0.5～20%, and cosolvent is one or more in methyl alcohol, isoamyl alcohol, phenol, ethyl acetate, the dimethyl sulfoxide (DMSO), use when multiple, total usage amount can not greater than deal maximum;
PH value conditioning agent accounts for preparation by weight 0.5～1%, and pH value conditioning agent is one or more in sodium hydroxide, ammonium hydroxide, acetic acid, the hydrochloric acid, use when multiple, total usage amount can not greater than deal maximum;
Synergist accounts for preparation by weight 0.5～25%, and synergist is one or more in Butacide, sulfoxide, alkyl carbonate, chlorination fat, the formaldehyde, use when multiple, total usage amount can not greater than deal maximum;
Anti-photodissociation agent accounts for preparation by weight 0.5～15%, and anti-photodissociation agent is one or more in p-aminobenzoic acid, castor oil, the Angelica oil, use when multiple, total usage amount can not greater than deal maximum;
Stabilizer comprises preparation by weight 0.5～15%, stabilizing agent is one or more in epoxidized soybean oil, the epoxidized linseed, use when multiple, total usage amount can not greater than deal maximum;
Thickener accounts for preparation by weight 0.1～2%, and thickener is one or more in silica, the bentonite, use when multiple, total usage amount can not greater than deal maximum;
Or when the preparation suspended emulsion, its auxiliary agent is:
Surfactant comprise preparation by weight 5～30%; Surfactant is the polyethenoxy ether class of nonionic; Or the sodium alkyl sulfate of anionic or in the alkyl benzene sulfonate one or more, use when multiple, total usage amount can not greater than deal maximum;
Solvent accounts for preparation by weight 10～80%; Solvent is various aromatic hydrocarbons, aliphatic hydrocarbon, ketone, ethers; In available benzene,toluene,xylene, naphthalane, gasoline, kerosene, machine oil, MEK, propylene glycol, butyl glycol ether, the benzinum one or more; Use when multiple, total usage amount can not greater than deal maximum;
Cosolvent accounts for preparation by weight 0.5～20%, and cosolvent is one or more in methyl alcohol, isoamyl alcohol, phenol, ethyl acetate, the dimethyl sulfoxide (DMSO), use when multiple, total usage amount can not greater than deal maximum;
PH value conditioning agent accounts for preparation by weight 0.5～1%, and pH value conditioning agent is one or more in sodium hydroxide, ammonium hydroxide, acetic acid, the hydrochloric acid, use when multiple, total usage amount can not greater than deal maximum;
Synergist accounts for preparation by weight 0.5～25%, and synergist is one or more in Butacide, sulfoxide, alkyl carbonate, chlorination fat, the formaldehyde, use when multiple, total usage amount can not greater than deal maximum;
Anti-photodissociation agent accounts for preparation by weight 0.5～15%, and anti-photodissociation agent is one or more in p-aminobenzoic acid, castor oil, the Angelica oil, use when multiple, total usage amount can not greater than deal maximum;
Stabilizer comprises preparation by weight 0.5～15%, stabilizing agent is one or more in epoxidized soybean oil, the epoxidized linseed, use when multiple, total usage amount can not greater than deal maximum;
Thickener accounts for preparation by weight 0.1～2%, and thickener is one or more in silica, the bentonite, use when multiple, total usage amount can not greater than deal maximum;
Or when the preparation aqueous emulsion, its auxiliary agent is:
Solvent accounts for preparation by weight 5～70%, and solvent is one or more in alcohols, ketone, ester class and the aromatic hydrocarbon, use when multiple, total usage amount can not greater than deal maximum;
Emulsifier accounts for preparation by weight 5～30%; Emulsifier is alkyl benzene sulfonate calcium salt, alkyl benzene sulphonate magnesium salts, alkyl benzene sulphonate aluminium salt, alkyl benzene sulphonate sodium salt, alkyl benzene sulphonate barium salt, C8～C20 alkylsurfuric acid sodium salt, styrene polyoxyethylene ether ammonium sulfate salt, benzylbiphenyl phenol polyethenoxy ether, phenethyl phenol polyethenoxy (N=15～30) ether, phenethyl phenol polyethenoxy polyoxypropylene, NPE, APES first condensation product, xenol APEO, homemade Nongru-700 number, in No. 300 one or more; Use when multiple, total usage amount can not greater than deal maximum;
Stabilizer comprises preparation by weight 0.5～5%; Stabilizing agent is a 3-chloro-1; In 2-expoxy propane, butyl glycidyl ether, phenyl glycidyl ether, cresyl glycidyl ether, polyvinyl one glycol diglycidyl ether, the sodium sorbate one or more; Use when multiple, total usage amount can not greater than deal maximum;
Antifreezing agent accounts for preparation by weight 0.5～10%, and antifreezing agent is one or more in ethylene glycol, glycerine, propane diols, polyethylene glycol, the sorb sodium alkoxide, use when multiple, total usage amount can not greater than deal maximum;
The preparation method of said missible oil is: by proportioning metalaxyl and the former medicine of Tea Saponin are dissolved in the solvent; Other auxiliary agents such as emulsifier are joined in metalaxyl and the former drug solns of Tea Saponin; Stir, put at last and give intense mixing in the homogenizer and homogenize promptly gets.
The preparation method of said wetting powder is: by proportioning metalaxyl and the former medicine of Tea Saponin are carried out joining in the blender behind the comminution by gas stream, in blender, add auxiliary agent respectively by proportioning, stir promptly and get.
The preparation method of said microemulsion is: metalaxyl is dissolved in the solvent; Tea Saponin is soluble in water, emulsifier is joined in the metalaxyl solution, stir; Add cosolvent, the Tea Saponin aqueous solution then, put at last and give intense mixing in the homogenizer and homogenize promptly gets.
The preparation method of said suspending agent is: by proportioning the former medicine of metalaxyl and Tea Saponin is added wetting dispersing agent, thickener, stabilizing agent, antifoaming agent, antifreezing agent, pH regulator agent and other auxiliary agent and deionized water one and arise from the ball mill ball milling and process suspending agent and promptly get.
The preparation method of said suspended emulsion is: by proportioning metalaxyl and the former medicine of Tea Saponin are pulverized; Add various auxiliary agents and water except that thickener again; Get into together to mix in the high-shear emulsion machine and add thickener simultaneously and further mix;, promptly get after microscopy is qualified after sand mill grinds.
The preparation method of said aqueous emulsion is: by ratio requirement metalaxyl and the former medicine of Tea Saponin are dissolved in the solvent, emulsifier, cosolvent are joined in metalaxyl and the former drug solns of Tea Saponin, stir, slowly add then in the entry, fully stirring promptly gets.
Bactericide of the present invention can be applicable to prevent and treat the plant disease that oomycetes, sac fungi, basidiomycetes, imperfect fungus cause.Like the powdery mildew of careless strain on wheat, paddy rice, cereal, apple, grape, strawberry, cucumber, capsicum, eggplant, Chinese cabbage, tobacco, banana, oranges and tangerines and flowers, the lawn, leaf mold, ring spot, leaf spot, anthracnose etc.; Comprise that banana anthracnose, banana crown rot, strawberry leaves mildew, purple blotch of onion, rice sheath blight disease, graw mold of tomato, peronophythora litchi, watermelon blight, rice blast, corn base rot disease, the corn northern leaf blight, sigatoka, root rotof flax, cotton verticillium wilt, cucumber eqpidemic disease are sick, lettuce stalk break or cotton wilt, for the production of agricultural, pollution-free food, nuisanceless fruits and vegetables and the green plants of beautifying the environment provide safe and efficient pesticide new variety.
Beneficial effect of the present invention is:
1, the present invention selects metalaxyl and Tea Saponin to carry out composite; Through a large amount of experiment confirm Tea Saponins when self has bactericidal action; Through improving the opening up of soup, wetting action, metalaxyl is had significant synergies, the generation of the antagonism property of medicine has retarding action.
2, bactericidal composition bactericidal activity of the present invention obviously strengthens, and multiple diseases is played the double effect of controlling of a medicine, prevents and treats the manpower and materials input in the disease process thereby reduce, and improves productivity effect.
3, use the metalaxyl cost higher separately, and the Tea Saponin cost is very low, adopts these two kinds of single agent composite, cost obviously reduces under the prerequisite that guarantees drug effect.
4, the present invention adopts conventional method can prepare multiple formulation, and the preparation method is simple, and promotional value is very high.
Embodiment:
Below in conjunction with embodiment the present invention is further described.The present invention obtains the compatibility effect of Tea Saponin and metalaxyl through long-term a large amount of experimental summaries, and the auxiliary agent of conventional use is not given unnecessary details one by one in an embodiment, but does not therefore limit the scope of the invention.
Embodiment 1 preparation 3% Tea Saponin metalaxyl microemulsion, the weight proportion of each component is:
Metalaxyl 0.6%, Tea Saponin 2.4%, methyl alcohol 10%, cyclohexane 9%, ethyl acetate 8%, cyclohexanone 25%, farming breast 500 4%, Nongru-700 8%, AEO-7 10%, GP3300.5%, glycerine 0.5%, the water of surplus.By proportioning metalaxyl and Tea Saponin are dissolved in methyl alcohol, cyclohexane, the ethyl acetate, mix other composition modulation of back adding and obtain microemulsion.
Embodiment 2: prepare 5% Tea Saponin metalaxyl (tea first) missible oil, the weight proportion of each component is:
Metalaxyl 2.5%; Tea Saponin 3.0%; Surfactant farming breast 500 is 10%, and Nongru-700 is 10%; Organic solvent cyclohexane 20%, ethyl acetate 10%, DMF10%, carrene 10%; Cosolvent methyl alcohol 5%; Stabilizing agent castor oil 5%; Anti-photodissociation agent epoxidized soybean oil 5%; Penetrating agent Laurocapram 10%.Each component is put into modulation kettle mixes well promptly and gets.Embodiment 3: prepare 80.5% Tea Saponin metalaxyl (tea first) wetting powder, the weight proportion of each component is:
Metalaxyl 0.5%; Tea Saponin 80%; Wetting agent bay alcohol radical sodium sulphate 3%; Dispersant lignin 2.5%; Filler diatomite 14%.By proportioning metalaxyl and the former medicine of Tea Saponin are carried out joining in the blender behind the comminution by gas stream, in blender, add auxiliary agent respectively, stir promptly and get by proportioning;
Embodiment 4: prepare 80.01% Tea Saponin metalaxyl (tea first) suspending agent, the weight proportion of each component is:
Metalaxyl 80%; Tea Saponin 0.01%; Surfactant sodium alkyl sulfate 7.39%; Solvent propylene glycol 10%; Cosolvent dimethyl sulfoxide (DMSO) 0.5%; PH value conditioning agent acetic acid 0.5%; Synergist Butacide 0.5%; Anti-photodissociation agent castor oil 0.5%; Stabilizing agent epoxidized soybean oil 0.5%; Thickener-silica 0.1%.By proportioning metalaxyl and the former medicine of Tea Saponin are added in the above-mentioned auxiliary agent and to arise from the ball mill ball milling with deionized water one and process suspending agent and promptly get;
Embodiment 5: prepare 10% Tea Saponin metalaxyl (tea first) suspended emulsion, the weight proportion of each component is:
Metalaxyl 5%; Tea Saponin 5%; Surfactant Nongru-700 30%; Solvent toluene 52%; Cosolvent dimethyl sulfoxide (DMSO) 5%; PH value conditioning agent acetic acid 0.5%; Synergist Butacide 0.5%; Anti-photodissociation agent castor oil 0.5%; Stabilizing agent epoxidized soybean oil 0.5%; Thickener-silica 1%.By proportioning metalaxyl and the former medicine of Tea Saponin are pulverized, added auxiliary agent and water except that thickener, in high-shear emulsion machine, mix the adding thickener, mix, grind, promptly get after microscopy is qualified through sand mill;
Embodiment 6: prepare 30% Tea Saponin metalaxyl (tea first) aqueous emulsion, the weight proportion of each component is:
Metalaxyl 29.5%; Tea Saponin 0.5%; Solvent acetone 50%; Emulsifier NPE 10%; Stabilizing agent sodium sorbate 3%; Antifreezing agent polyethylene glycol 7%.By ratio requirement metalaxyl and the former medicine of Tea Saponin are dissolved in the solvent, emulsifier, cosolvent are joined in metalaxyl and the former drug solns of Tea Saponin, slowly add after stirring in the entry, fully stirring promptly gets.Embodiment 7 bactericidal compositions of the present invention are estimated sigatoka bacterium co-toxicity
Adopt the mycelial growth rate method to measure embodiment 1～6 bactericidal composition preparation to the synergy of sigatoka bacterium, according to the synergic ratio SR value of calculating composite dose according to the method for Wadley.A, B represent two kinds of medicament components respectively, and a, b are the ratio of A, B two components content in mixture.Analyze the effect of mixture with the SR value.
Criterion
There is antagonism SR≤0.5 for composite dose
SR:0.5-1.5 has summation action for composite dose
There is synergistic effect SR >=1.5 for composite dose
Experimental result is seen table 1.
Table 1 Tea Saponin and metalaxyl are mixed to the co-toxicity experimental result of sigatoka bacterium
Embodiment 8: bactericidal composition of the present invention is to the bacteriostatic activity of 17 kinds of pathogens
Adopt the mycelial growth rate method to measure the inhibition effect of embodiment 2 bactericidal composition preparations to 17 kinds of disease funguses.Under aseptic condition, preparation variable concentrations medicament is poured in the medium (temperature is about 60 ℃) that has melted again, fully shakes up, and pours into while hot in the culture dish of 90cm and processes the pastille culture medium flat plate.Flat board solidifies cooling back and meets the various plant pathogenic fungi bacterium cakes that into size is consistent, growth is consistent (Φ=5.0mm) with transfer needle; Every ware connects 1 bacterium cake; Growth has one of bacterium colony to face down, and clear water blank, the contrast of 1% methyl alcohol are established in 3 repetitions of every processing simultaneously.Place biochemical incubator, cultivated 5 days under 25 ℃ of conditions, measure the size that supplies examination fungus colony diameter with the right-angled intersection method again, calculate bacteriostasis rate, and then calculate chemicals treatment concentration (EC in effective inhibition of different germs by following formula
50) and correlation coefficient (r) value.
Bacteriostatic activity is measured the result and is seen table 2.
Table 2 Tea Saponin and metalaxyl are mixed to the bacteriostatic activity of 17 plant species disease funguses
Embodiment 9: bactericidal composition of the present invention is to sigatoka bacterium indoor pot evaluating drug effect
In the polypots of high 7.5cm diameter 9cm, load onto the riverbed soil of not using agricultural chemicals, mix farmyard manure, the banana seedlings (six leaves wholeheartedly) that plantation academy of agricultural sciences, Guangdong Province cultivates is cultivated in the greenhouse, imposes nitrogenous fertilizer in the process of growth, makes blade fresher and tenderer easy to be susceptible.Be mixed with sigatoka bacterium spore suspension with sterile distilled water, inoculum density is microscope 10 * 10 times, 80～100 spore/visuals field.The spray inoculation banana seedlings, 5mL spore suspension/alms bowl, then, banana seedlings is at T:23 ℃, and the dark condition of RH＞95% was cultivated 24 hours down, and the soup of the variable concentrations for preparing is sprayed to banana seedlings, and dose is as the criterion when blade begins to drip to be sprayed onto.Banana seedlings is placed T:23 ± 20 ℃, RH＞95%, periodicity of illumination 12: 12h, and (L: cultivate under condition D), after 7 days, when contrast obviously during morbidity, investigation result, control efficiency is calculated with reference to following formula.And calculate the control regression curve, ask for PET
50(effectively concentration in the control).
Result's investigation is a unit with the blade, and grade scale is following:
0 grade: no scab;
1 grade: lesion area＜1/6;
3 grades: lesion area 1/6～1/3;
5 grades: lesion area 1/3～1/2;
7 grades: lesion area＞1/2;
Experimental result is seen table 3.
Claims (3)
1. metalaxyl and the composite bactericidal composition of Tea Saponin is characterized in that comprising following components in weight percentage:
Metalaxyl: 0.5～80%;
Tea Saponin: 0.01～80%;
The auxiliary agent surplus;
Said bactericidal composition is applied to prevent and treat the plant disease that oomycetes, sac fungi, basidiomycetes, imperfect fungus cause; Said plant disease comprises banana anthracnose, banana crown rot, strawberry leaves mildew, purple blotch of onion, rice sheath blight disease, graw mold of tomato, peronophythora litchi, watermelon blight, rice blast, corn base rot disease, the corn northern leaf blight, sigatoka, root rotof flax, cotton verticillium wilt, cucumber eqpidemic disease disease, lettuce stalk break or cotton wilt.
2. according to said metalaxyl of claim 1 and the composite bactericidal composition of Tea Saponin, it is characterized in that bactericidal composition is missible oil, wetting powder, microemulsion, suspending agent, suspended emulsion, aqueous emulsion or soluble liquid.
3. the preparation method of the composite bactericidal composition of said metalaxyl of claim 2 and Tea Saponin is characterized in that:
The preparation method of said missible oil is: by proportioning metalaxyl and the former medicine of Tea Saponin are dissolved in the solvent, auxiliary agent is joined in metalaxyl and the former drug solns of Tea Saponin, stir, put into and give intense mixing in the homogenizer and homogenize promptly gets;
The preparation method of said wetting powder is: by proportioning metalaxyl and the former medicine of Tea Saponin are carried out joining in the blender behind the comminution by gas stream, in blender, add auxiliary agent respectively by proportioning, stir promptly and get;
The preparation method of said microemulsion is: metalaxyl is dissolved in the solvent, emulsifier is joined in the metalaxyl solution, stir; The Tea Saponin Tea Saponin aqueous solution that gets soluble in water; Cosolvent, the Tea Saponin aqueous solution are added metalaxyl solution, stir, put into and give intense mixing in the homogenizer and homogenize promptly gets;
The preparation method of said suspending agent is: by proportioning the former medicine of metalaxyl and Tea Saponin is added in the auxiliary agent and arise from the ball mill ball milling with deionized water one and process suspending agent and promptly get;
The preparation method of said suspended emulsion is: by proportioning metalaxyl and the former medicine of Tea Saponin are pulverized; Add auxiliary agent and water except that thickener, in high-shear emulsion machine, mix the adding thickener, mix; Grind through sand mill, promptly get after microscopy is qualified;
The preparation method of said aqueous emulsion is: by ratio requirement metalaxyl and the former medicine of Tea Saponin are dissolved in the solvent, emulsifier, cosolvent are joined in metalaxyl and the former drug solns of Tea Saponin, slowly add after stirring in the entry, fully stirring promptly gets.
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| AU2015271938B2 (en) * | 2010-09-24 | 2017-07-27 | Bayer Intellectual Property Gmbh | Fungicidal compositions and methods of use |
| EP2618659A4 (en) * | 2010-09-24 | 2014-04-02 | Bayer Ip Gmbh | Fungicidal compositions and methods of use |
| CN102763646A (en) * | 2012-06-30 | 2012-11-07 | 广东中迅农科股份有限公司 | Metalaxyl emulsion in water and preparation method of metalaxyl emulsion in water |
| CN105165898B (en) * | 2015-10-26 | 2018-04-24 | 广西南宁益土生物科技有限责任公司 | A kind of granule containing metalaxyl and trichoderma harzianum |
| CN107087611A (en) * | 2017-05-08 | 2017-08-25 | 广西南宁益土生物科技有限责任公司 | A kind of bactericidal composition containing Chinese yam polysaccharide and zineb |
| CN108371186A (en) * | 2018-04-23 | 2018-08-07 | 张月婵 | Bactericidal composition containing azalomycin F and metalaxyl |
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| CN101161069A (en) * | 2007-09-21 | 2008-04-16 | 华南农业大学 | Insecticidal composition of Chinese azalea extract III and theasaponine |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101161069A (en) * | 2007-09-21 | 2008-04-16 | 华南农业大学 | Insecticidal composition of Chinese azalea extract III and theasaponine |
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