CN101543219A - Sterilizing composite compounded by diniconazole and tea saponin, preparation method and application thereof - Google Patents
Sterilizing composite compounded by diniconazole and tea saponin, preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a sterilizing composite compounded by diniconazole and tea saponin, a preparation method and the application thereof. The sterilizing composite comprises the following components according to the weight percent: 0.5-80 of diniconazole, 0.01-80 of tea saponin, and the balance of assistant. The sterilizing composite can be prepared to missible oil, wettable powder, microemulsion, suspending agent, suspending emulsion, aqueous emulsion or dissoluble liquid. The invention is compounded by the diniconazole and the tea saponin, has remarkable synergized action and more obviously strengthened sterilizing activity than the single sterilant, and plays a role of one medicine for treating multiple diseases, thereby reducing manpower and material investment during the disease prevention, improving the production profit, and obviously lowering the cost under the premise of ensuring the medicine effect. The invention has multiple formulations, simple preparation method and higher popularization value.
Description
Technical field
The invention belongs to agricultural bactericidal preparation technique field, be specifically related to a kind of bactericidal composition its preparation method and application of compounded by diniconazole and tea saponin.
Background technology
Because long-term, a large amount of unreasonable uses of chemical pesticide, and because the intrinsic shortcoming of some medicament itself has caused serious pesticide resistance problem.It is good to seek Environmental compatibility, and the public nuisance-free agricultural chemicals to pathogen has lasting effect seems especially important in today that pollution-free food is much accounted of day by day.
Natural products often shows many biologically actives, and widely people are used for hundreds of years.Wherein, the research and development of plant resource fungicide active ingredient is an important research direction that solves the many negative effects of current chemical bactericide.Therefore, be object with the natural products, seek bioactivator, the research and development novel biopesticide enjoys attention at home and abroad, and wherein, botanical pesticide is a most noticeable class.Being mixed of the exploitation of botanical pesticide, botanical pesticide and chemical pesticide, botanical pesticide and being mixed of botanical pesticide all are the forward position and the focus of pesticide research in the world.
Alkene azoles alcohol is wide-spectrum bactericide, belong to triazole bactericidal agent, spray and can prevent the smelting smut of being scattered about like the stars on grape, cereal and fruit, this bactericide also can be used to the powdery mildew of anti-smelting rose, the leaf spot of peanut, the leaf spot of banana and the powdery mildew of coffee, in the ergosterol biosynthesis of fungi, suppress 14 α-demethylation effect, cause that ergosterol lacks, and causes fungal cell membrane undesired, final fungi death, the lasting period is permanent.
Tea Saponin (Tea saponin) is the bactericide that extracts from the tea cake after the seed of plant of theaceae such as tea seed cake, the tea-seed oil expression, belongs to pentacyclic triterpenoid, and its pure product are colorless prismatic crystal, have hygroscopicity, and bitter is pungent, and molecular formula is C
57H
90O
26, molecular weight is 1191.31,223～224 ℃ of fusing points, and soluble in water and organic solvents such as alcohols, organic acid are insoluble to organic solvents such as ether, benzinum, benzene.
Tea Saponin is a kind of nonionic surface active agent, has that stronger exhibition is puted forth effort, emulsifying ability, dispersion force, wetting power and a foaming power, and cold-blooded animal is had high poison, and people and animals are waited the nontoxic or low toxicity of homoiothermy animal.This compound has sterilization, sterilization, kills mite and reduces capillary multiple function, has different mechanism of action with chemical pesticide, and by improve soup wetting, opening up, emulsifying dispersivity has significant synergies to chemical pesticide.Tea Saponin is typical no-pollution multifunctional plant pesticide kind, and for example at Chinese countryside, the tea bran always is exactly a soil pesticide, can kill oncomelania as pool-cleaning agent, makes bactericide with immersion tea bran water and can prevent rice blast and banded sclerotial blight.After deliberation, Tea Saponin itself has good inhibition effect to the various plants growth of pathogenic bacteria, is a kind of typical multi-functional plant source pesticide kind.But this medicine quick-acting is poor, and application dosage is very big when using separately.
Summary of the invention
The objective of the invention is to overcome the deficiency of existing bactericide technology, make full use of the unique effect mechanism of rich resources of tea saponin and its botanical pesticide, determine the suitable compatibility of itself and alkene azoles alcohol, a kind of efficient, wide spectrum is provided, is difficult for developing immunity to drugs, the bactericidal composition of low toxicity.
Another object of the present invention provides the application of described bactericidal composition.
Purpose of the present invention is achieved by the following technical programs:
A kind of bactericidal composition of compounded by diniconazole and tea saponin is provided, comprises following components in weight percentage:
Alkene azoles alcohol: 0.5～80%;
Tea Saponin: 0.01～80%;
The auxiliary agent surplus.
Described bactericide is missible oil, wetting powder, microemulsion, suspending agent, suspended emulsion, aqueous emulsion or soluble liquid, and the preparation method who is adopted is the method that adopts usually during corresponding formulation is produced.
Described auxiliary agent is determined according to formulation:
When preparation missible oil, its auxiliary agent is:
Surfactant comprise preparation by weight 5～30%, surfactant is the polyethenoxy ether class of nonionic, or the sodium alkyl sulfate of anionic or in the alkyl benzene sulfonate one or more, use when multiple, total usage amount can not greater than deal maximum;
Solvent accounts for preparation by weight 10～80%, solvent is various aromatic hydrocarbons, aliphatic hydrocarbon, ketone, ethers, in available benzene,toluene,xylene, naphthalane, gasoline, kerosene, machine oil, MEK, propylene glycol, butyl glycol ether, the benzinum one or more, use when multiple, total usage amount can not greater than deal maximum;
Cosolvent accounts for preparation by weight 0.5～20%, and cosolvent is one or more in methyl alcohol, isoamyl alcohol, phenol, ethyl acetate, the dimethyl sulfoxide (DMSO), use when multiple, total usage amount can not greater than deal maximum;
PH value conditioning agent accounts for preparation by weight 0.5～1%, and pH value conditioning agent is one or more in sodium hydroxide, ammonium hydroxide, acetic acid, the hydrochloric acid, use when multiple, total usage amount can not greater than deal maximum;
Synergist accounts for preparation by weight 0.5～25%, and synergist is one or more in Butacide, sulfoxide, alkyl carbonate, chlorination fat, the formaldehyde, use when multiple, total usage amount can not greater than deal maximum;
Anti-photodissociation agent accounts for preparation by weight 0.5～15%, and anti-photodissociation agent is one or more in p-aminobenzoic acid, castor oil, the Angelica oil, use when multiple, total usage amount can not greater than deal maximum;
Stabilizer comprises preparation by weight 0.5～15%, stabilizing agent is one or more in epoxidized soybean oil, the epoxidized linseed, use when multiple, total usage amount can not greater than deal maximum;
Penetrating agent accounts for preparation by weight 0.5～2%, and penetrating agent is one or more in Laurocapram, JFC-2, the maleic acid di-sec-octyl sodium sulfonate, use when multiple, total usage amount can not greater than deal maximum;
Or when the preparation wetting powder, its auxiliary agent is:
Wetting agent accounts for preparation by weight 2.5～35%, wetting agent is natural products wetting agent and anionic wetting agent, available tea is withered, spaonin powder, soapberry powder, silkworm excrement, bay alcohol radical sodium sulphate, neopelex, washing powder, draw back in the powder one or more, use when multiple, total usage amount can not greater than deal maximum;
Dispersant accounts for preparation by weight 2.5～30%, dispersant is industrial by-products and anionic dispersing agent, in available lignosulfite, lignin and derivative sulfonate, naphthalene and alkylbenzene formaldehyde condensation products sulfonate, the alkylsulfonate one or more, use when multiple, total usage amount can not greater than deal maximum;
Filler accounts for preparation by weight 10～70%, and filler is mineral type carrier or synthetic vectors, and one or more in available kaolin, diatomite, the white carbon use when multiple, total usage amount can not greater than deal maximum;
Or when the preparation microemulsion, its auxiliary agent is:
Solvent accounts for preparation by weight 10～80%, and solvent is one or more in alcohols, ketone, ester class and the aromatic hydrocarbon, use when multiple, total usage amount can not greater than deal maximum;
Emulsifier accounts for preparation by weight 5～30%, emulsifier is an alkyl benzene sulfonate calcium salt, the alkyl benzene sulphonate magnesium salts, alkyl benzene sulphonate aluminium salt, the alkyl benzene sulphonate sodium salt, the alkyl benzene sulphonate barium salt, C8～C20 alkylsurfuric acid sodium salt, styrene polyoxyethylene ether ammonium sulfate salt, the benzylbiphenyl phenol polyethenoxy ether, phenethyl phenol polyethenoxy (N=15～30) ether, phenethyl phenol polyethenoxy polyoxypropylene, polyoxyethylene nonylphenol ether, alkylphenol polyoxyethylene first condensation product, the xenol APEO, homemade Nongru-700 number, in No. 300 one or more, use when multiple, total usage amount can not greater than deal maximum;
Stabilizer comprises preparation by weight 0.5～15%, stabilizing agent is a 3-chloro-1, in 2-expoxy propane, butyl glycidyl ether, phenyl glycidyl ether, cresyl glycidyl ether, polyvinyl one glycol diglycidyl ether, the sodium sorbate one or more, use when multiple, total usage amount can not greater than deal maximum;
Antifreezing agent accounts for preparation by weight 0.5～10%, and antifreezing agent is one or more in ethylene glycol, glycerine, propane diols, polyethylene glycol, the sorb sodium alkoxide, use when multiple, total usage amount can not greater than deal maximum;
Or when the preparation suspending agent, its auxiliary agent is:
Surfactant comprise preparation by weight 5～30%, surfactant is the polyethenoxy ether class of nonionic, or the sodium alkyl sulfate of anionic or in the alkyl benzene sulfonate one or more, use when multiple, total usage amount can not greater than deal maximum;
Solvent accounts for preparation by weight 10～80%, solvent is various aromatic hydrocarbons, aliphatic hydrocarbon, ketone, ethers, in available benzene,toluene,xylene, naphthalane, gasoline, kerosene, machine oil, MEK, propylene glycol, butyl glycol ether, the benzinum one or more, use when multiple, total usage amount can not greater than deal maximum;
Cosolvent accounts for preparation by weight 0.5～20%, and cosolvent is one or more in methyl alcohol, isoamyl alcohol, phenol, ethyl acetate, the dimethyl sulfoxide (DMSO), use when multiple, total usage amount can not greater than deal maximum;
PH value conditioning agent accounts for preparation by weight 0.5～1%, and pH value conditioning agent is one or more in sodium hydroxide, ammonium hydroxide, acetic acid, the hydrochloric acid, use when multiple, total usage amount can not greater than deal maximum;
Synergist accounts for preparation by weight 0.5～25%, and synergist is one or more in Butacide, sulfoxide, alkyl carbonate, chlorination fat, the formaldehyde, use when multiple, total usage amount can not greater than deal maximum;
Anti-photodissociation agent accounts for preparation by weight 0.5～15%, and anti-photodissociation agent is one or more in p-aminobenzoic acid, castor oil, the Angelica oil, use when multiple, total usage amount can not greater than deal maximum;
Stabilizer comprises preparation by weight 0.5～15%, stabilizing agent is one or more in epoxidized soybean oil, the epoxidized linseed, use when multiple, total usage amount can not greater than deal maximum;
Thickener accounts for preparation by weight 0.1～2%, and thickener is one or more in silica, the bentonite, use when multiple, total usage amount can not greater than deal maximum;
Or when the preparation suspended emulsion, its auxiliary agent is:
Surfactant comprise preparation by weight 5～30%, surfactant is the polyethenoxy ether class of nonionic, or the sodium alkyl sulfate of anionic or in the alkyl benzene sulfonate one or more, use when multiple, total usage amount can not greater than deal maximum;
Solvent accounts for preparation by weight 10～80%, solvent is various aromatic hydrocarbons, aliphatic hydrocarbon, ketone, ethers, in available benzene,toluene,xylene, naphthalane, gasoline, kerosene, machine oil, MEK, propylene glycol, butyl glycol ether, the benzinum one or more, use when multiple, total usage amount can not greater than deal maximum;
Cosolvent accounts for preparation by weight 0.5～20%, and cosolvent is one or more in methyl alcohol, isoamyl alcohol, phenol, ethyl acetate, the dimethyl sulfoxide (DMSO), use when multiple, total usage amount can not greater than deal maximum;
PH value conditioning agent accounts for preparation by weight 0.5～1%, and pH value conditioning agent is one or more in sodium hydroxide, ammonium hydroxide, acetic acid, the hydrochloric acid, use when multiple, total usage amount can not greater than deal maximum;
Synergist accounts for preparation by weight 0.5～25%, and synergist is one or more in Butacide, sulfoxide, alkyl carbonate, chlorination fat, the formaldehyde, use when multiple, total usage amount can not greater than deal maximum;
Anti-photodissociation agent accounts for preparation by weight 0.5～15%, and anti-photodissociation agent is one or more in p-aminobenzoic acid, castor oil, the Angelica oil, use when multiple, total usage amount can not greater than deal maximum;
Stabilizer comprises preparation by weight 0.5～15%, stabilizing agent is one or more in epoxidized soybean oil, the epoxidized linseed, use when multiple, total usage amount can not greater than deal maximum;
Thickener accounts for preparation by weight 0.1～2%, and thickener is one or more in silica, the bentonite, use when multiple, total usage amount can not greater than deal maximum;
Or when the preparation aqueous emulsion, its auxiliary agent is:
Solvent accounts for preparation by weight 5～70%, and solvent is one or more in alcohols, ketone, ester class and the aromatic hydrocarbon, use when multiple, total usage amount can not greater than deal maximum;
Emulsifier accounts for preparation by weight 5～30%, emulsifier is an alkyl benzene sulfonate calcium salt, the alkyl benzene sulphonate magnesium salts, alkyl benzene sulphonate aluminium salt, the alkyl benzene sulphonate sodium salt, the alkyl benzene sulphonate barium salt, C8～C20 alkylsurfuric acid sodium salt, styrene polyoxyethylene ether ammonium sulfate salt, the benzylbiphenyl phenol polyethenoxy ether, phenethyl phenol polyethenoxy (N=15～30) ether, phenethyl phenol polyethenoxy polyoxypropylene, polyoxyethylene nonylphenol ether, alkylphenol polyoxyethylene first condensation product, the xenol APEO, homemade Nongru-700 number, in No. 300 one or more, use when multiple, total usage amount can not greater than deal maximum;
Stabilizer comprises preparation by weight 0.5～5%, stabilizing agent is a 3-chloro-1, in 2-expoxy propane, butyl glycidyl ether, phenyl glycidyl ether, cresyl glycidyl ether, polyvinyl one glycol diglycidyl ether, the sodium sorbate one or more, use when multiple, total usage amount can not greater than deal maximum;
Antifreezing agent accounts for preparation by weight 0.5～10%, and antifreezing agent is one or more in ethylene glycol, glycerine, propane diols, polyethylene glycol, the sorb sodium alkoxide, use when multiple, total usage amount can not greater than deal maximum;
The preparation method of described missible oil is: by proportioning the former medicine of the pure and mild Tea Saponin of alkene azoles is dissolved in the solvent, other auxiliary agents such as emulsifier are joined in the former drug solns of the pure and mild Tea Saponin of alkene azoles, stir, put at last and mix also homogenize in the homogenizer strongly promptly.
The preparation method of described wetting powder is: by proportioning the former medicine of the pure and mild Tea Saponin of alkene azoles is carried out joining in the blender behind the comminution by gas stream, add auxiliary agent respectively by proportioning in blender, stir promptly.
The preparation method of described microemulsion is: alkene azoles alcohol is dissolved in the solvent, Tea Saponin is soluble in water, emulsifier is joined in the alkene azoles alcoholic solution, stir, add cosolvent, the Tea Saponin aqueous solution then, put at last and mix also homogenize in the homogenizer strongly promptly.
The preparation method of described suspending agent is: by proportioning the former medicine of the pure and mild Tea Saponin of alkene azoles is added wetting dispersing agent, thickener, stabilizing agent, defoamer, antifreezing agent, pH regulator agent and other auxiliary agent and deionized water one and arise from the ball mill ball milling and make suspending agent promptly.
The preparation method of described suspended emulsion is: by proportioning the former medicine of the pure and mild Tea Saponin of alkene azoles is pulverized, add various auxiliary agents and water except that thickener again, enter together to mix in the high-shear emulsion machine and add thickener simultaneously and further mix, after sand mill grind, after microscopy is qualified promptly.
The preparation method of described aqueous emulsion is: by ratio requirement the former medicine of the pure and mild Tea Saponin of alkene azoles is dissolved in the solvent, emulsifier, cosolvent are joined in the former drug solns of the pure and mild Tea Saponin of alkene azoles, stir, slowly add then in the entry, fully stir promptly.
Bactericide of the present invention can be applicable to prevent and treat oomycetes, sac fungi, basidiomycetes and/plant disease that imperfect fungus causes.As wheat, paddy rice, cereal, apple, grape, strawberry, cucumber, capsicum, eggplant, Chinese cabbage, tobacco, banana, oranges and tangerines and flowers, the powdery mildew of careless strain on the lawn, leaf mold, ring spot, leaf spot, anthracnose etc., comprise banana anthracnose, the banana crown rot, the strawberry leaves mildew, purple blotch of onion, rice sheath blight disease, graw mold of tomato, peronophythora litchi, watermelon blight, rice blast, the corn base rot disease, the corn northern leaf blight, sigatoka, root rotof flax, cotton verticillium wilt, cucumber eqpidemic disease disease, lettuce stalk break and/or cotton wilt are agricultural, pollution-free food, the production of nuisanceless fruits and vegetables and the green plants of beautifying the environment provide safe and efficient pesticide new variety.
Beneficial effect of the present invention is:
1, the present invention selects the pure and mild Tea Saponin of alkene azoles to carry out composite, by a large amount of experiment confirm Tea Saponins when self has bactericidal action, by improving the opening up of soup, wetting action, alkene azoles alcohol is had significant synergies, the generation of the antagonism property of medicine has retarding action.
2, bactericidal composition bactericidal activity of the present invention obviously strengthens, and multiple diseases is played the double effect of controlling of a medicine, thereby reduces the manpower and materials input of preventing and treating in the disease process, improves productivity effect.
3, use alkene azoles alcohol cost higher separately, and the Tea Saponin cost is very low, adopts these two kinds of single agent composite, cost obviously reduces under the prerequisite that guarantees drug effect.
4, the present invention adopts conventional method can prepare multiple formulation, and the preparation method is simple, and promotional value is very high.
Embodiment:
The present invention is further illustrated below in conjunction with embodiment, and the present invention obtains the compatibility effect of Tea Saponin and alkene azoles alcohol by long-term a large amount of experimental summaries, and the conventional auxiliary agent that uses is not given unnecessary details one by one in an embodiment, but does not therefore limit the scope of the invention.
Embodiment 1 preparation 3% Tea Saponin alkene azoles alcohol (tea alkene) microemulsion, the weight proportion of each component is:
Alkene azoles alcohol 2.4%, Tea Saponin 0.6%, methyl alcohol 10%, cyclohexane 9%, ethyl acetate 8%, cyclohexanone 25%, farming breast 500 4%, Nongru-700 8%, AEO-7 10%, and GP330 0.5%, glycerine 0.5%, the water of surplus.By proportioning the pure and mild Tea Saponin of alkene azoles is dissolved in methyl alcohol, cyclohexane, the ethyl acetate, mixes other composition modulation of back adding and obtain microemulsion.
Embodiment 2: prepare 5% Tea Saponin alkene azoles alcohol (tea alkene) missible oil, the weight proportion of each component is:
Alkene azoles alcohol 3.5%; Tea Saponin 1.5%; Surfactant farming breast 500 is 10%, and Nongru-700 is 10%; Organic solvent cyclohexane 20%, ethyl acetate 10%, DMF10%, carrene 10%; Cosolvent methyl alcohol 5%; Stabilizing agent castor oil 5%; Anti-photodissociation agent epoxidized soybean oil 5%; Penetrating agent Laurocapram 10%.Each component is put into modulation kettle mixes well promptly.
Embodiment 3: prepare 80.5% Tea Saponin alkene azoles alcohol (tea alkene) wetting powder, the weight proportion of each component is:
Alkene azoles alcohol 0.5%; Tea Saponin 80%; Wetting agent bay alcohol radical sodium sulphate 3%; Dispersant lignin 2.5%; Filler diatomite 14%.By proportioning the former medicine of the pure and mild Tea Saponin of alkene azoles is carried out joining in the blender behind the comminution by gas stream, in blender, add auxiliary agent respectively, stir promptly by proportioning.
Embodiment 4: prepare 80.01% Tea Saponin alkene azoles alcohol (tea alkene) suspending agent, the weight proportion of each component is:
Alkene azoles alcohol 80%; Tea Saponin 0.01%; Surfactant sodium alkyl sulfate 7.39%; Solvent propylene glycol 10%; Cosolvent dimethyl sulfoxide (DMSO) 0.5%; PH value conditioning agent acetic acid 0.5%; Synergist Butacide 0.5%; Anti-photodissociation agent castor oil 0.5%; Stabilizing agent epoxidized soybean oil 0.5%; Thickener-silica 0.1%.The former medicine of the pure and mild Tea Saponin of alkene azoles is added in the above-mentioned auxiliary agent and deionized water one arises from the ball mill ball milling and makes suspending agent promptly by proportioning.
Embodiment 5: prepare 10% Tea Saponin alkene azoles alcohol (tea alkene) suspended emulsion, the weight proportion of each component is:
Alkene azoles alcohol 5%; Tea Saponin 5%; Surfactant Nongru-700 30%; Solvent toluene 52%; Cosolvent dimethyl sulfoxide (DMSO) 5%; PH value conditioning agent acetic acid 0.5%; Synergist Butacide 0.5%; Anti-photodissociation agent castor oil 0.5%; Stabilizing agent epoxidized soybean oil 0.5%; Thickener-silica 1%.By proportioning the former medicine of the pure and mild Tea Saponin of alkene azoles is pulverized, is added auxiliary agent and water except that thickener, in high-shear emulsion machine, mix the adding thickener, mix, through the sand mill grinding, after microscopy is qualified promptly.
Embodiment 6: prepare 30% Tea Saponin alkene azoles alcohol (tea alkene) aqueous emulsion, the weight proportion of each component is:
Alkene azoles alcohol 29.5%; Tea Saponin 0.5%; Solvent acetone 50%; Emulsifier polyoxyethylene nonylphenol ether 10%; Stabilizing agent sodium sorbate 3%; Antifreezing agent polyethylene glycol 7%.By ratio requirement the former medicine of the pure and mild Tea Saponin of alkene azoles is dissolved in the solvent, emulsifier, cosolvent are joined in the former drug solns of the pure and mild Tea Saponin of alkene azoles, slowly add after stirring in the entry, fully stir promptly.
Embodiment 7: bactericidal composition of the present invention is estimated the peronophythora litchi co-toxicity
Adopt the mycelial growth rate method to measure embodiment 1～6 bactericidal composition preparation to the synergy of peronophythora litchi, according to the synergic ratio SR value of calculating composite dose according to the method for Wadley.A, B represent two kinds of medicament components respectively, and a, b are the ratio of A, B two components content in mixture.Analyze the effect of mixture with the SR value.
Criterion
There is antagonism SR≤0.5 for composite dose
SR:0.5-1.5 has summation action for composite dose
There is synergistic effect SR 〉=1.5 for composite dose
Experimental result sees Table 1.
Table 1 Tea Saponin and alkene azoles alcohol are mixed to the co-toxicity of peronophythora litchi bacterium
Embodiment 8: bactericidal composition of the present invention is to the bacteriostatic activity of 17 kinds of pathogens
Adopt the mycelial growth rate method to measure the inhibition effect of embodiment 2 bactericidal composition preparations to 17 kinds of disease funguses.Under aseptic condition, preparation variable concentrations medicament is poured in the medium (temperature is about 60 ℃) that has melted again, fully shakes up, and pours into while hot in the culture dish of 90cm and makes the pastille culture medium flat plate.Flat board solidifies cooling back and taps into the various plant pathogenic fungi bacterium cakes that size is consistent, growth is consistent (Φ=5.0mm) with transfer needle, every ware connects 1 bacterium cake, growth has one of bacterium colony to face down, and clear water blank, the contrast of 1% methyl alcohol are established in 3 repetitions of every processing simultaneously.Place biochemical incubator, cultivated 5 days under 25 ℃ of conditions, measure for the size of trying the fungus colony diameter with the right-angled intersection method again, calculate bacteriostasis rate as follows, and then calculate chemicals treatment concentration (EC in effective inhibition of different germs
50) and correlation coefficient (r) value.
The bacteriostatic activity measurement result sees Table 2.
Table 2 Tea Saponin and alkene azoles alcohol mixture preparation are to the bacteriostatic activity of 18 plant species disease funguses
Claims (5)
1, a kind of bactericidal composition of compounded by diniconazole and tea saponin is characterized in that comprising following components in weight percentage:
Alkene azoles alcohol: 0.5～80%;
Tea Saponin: 0.01～80%;
The auxiliary agent surplus.
2,, it is characterized in that bactericidal composition is missible oil, wetting powder, microemulsion, suspending agent, suspended emulsion, aqueous emulsion or soluble liquid according to the bactericidal composition of the described compounded by diniconazole and tea saponin of claim 1.
3, the preparation method of the bactericidal composition of the described compounded by diniconazole and tea saponin of a kind of claim 2 is characterized in that:
The preparation method of described missible oil is: by proportioning the former medicine of the pure and mild Tea Saponin of alkene azoles is dissolved in the solvent, auxiliary agent is joined in the former drug solns of the pure and mild Tea Saponin of alkene azoles, stir, put into and mix also homogenize in the homogenizer strongly promptly;
The preparation method of described wetting powder is: by proportioning the former medicine of the pure and mild Tea Saponin of alkene azoles is carried out joining in the blender behind the comminution by gas stream, add auxiliary agent respectively by proportioning in blender, stir promptly;
The preparation method of described microemulsion is: alkene azoles alcohol is dissolved in the solvent, emulsifier is joined in the alkene azoles alcoholic solution, stir; The Tea Saponin Tea Saponin aqueous solution that gets soluble in water; Cosolvent, the Tea Saponin aqueous solution are added alkene azoles alcoholic solution, stir, put into and mix also homogenize in the homogenizer strongly promptly;
The preparation method of described suspending agent is: add the former medicine of the pure and mild Tea Saponin of alkene azoles in the auxiliary agent and deionized water one arises from the ball mill ball milling and makes suspending agent promptly by proportioning;
The preparation method of described suspended emulsion is: by proportioning the former medicine of the pure and mild Tea Saponin of alkene azoles is pulverized, add auxiliary agent and water except that thickener, in high-shear emulsion machine, mix the adding thickener, mix, grind through sand mill, after microscopy is qualified promptly;
The preparation method of described aqueous emulsion is: by ratio requirement the former medicine of the pure and mild Tea Saponin of alkene azoles is dissolved in the solvent, emulsifier, cosolvent are joined in the former drug solns of the pure and mild Tea Saponin of alkene azoles, slowly add after stirring in the entry, fully stir promptly.
4, the application of the bactericidal composition of the described compounded by diniconazole and tea saponin of a kind of claim 1 is characterized in that being applied to prevent and treat the plant disease that oomycetes, sac fungi, basidiomycetes and/or imperfect fungus cause.
5, according to the application of the composite bactericidal composition of the described mancozeb of claim 4 and Tea Saponin, it is characterized in that being applied to prevent and treat plant banana anthracnose, banana crown rot, strawberry leaves mildew, purple blotch of onion, rice sheath blight disease, graw mold of tomato, peronophythora litchi, watermelon blight, rice blast, corn base rot disease, the corn northern leaf blight, sigatoka, root rotof flax, cotton verticillium wilt, cucumber eqpidemic disease disease, lettuce stalk break and/or cotton wilt.
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Cited By (2)
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|---|---|---|---|---|
| CN104255801A (en) * | 2014-08-29 | 2015-01-07 | 浙江大洋生物科技集团股份有限公司 | Crop potassium supplement composition with sterilization function as well as preparation method and application of crop potassium supplement composition |
| CN107027768A (en) * | 2017-04-28 | 2017-08-11 | 山东沃森农业科技有限公司 | A kind of bactericide for being used to prevent and treat dendrobium candidum southern blight |
Family Cites Families (3)
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| JPS6053018B2 (en) * | 1977-09-07 | 1985-11-22 | 住友化学工業株式会社 | Azole compounds, their production methods, and fungicides comprising the compounds |
| CN101081036B (en) * | 2006-05-29 | 2011-11-16 | 河南农业大学 | Bactericidal agent for preventing disease and increasing production |
| CN101194626A (en) * | 2006-12-26 | 2008-06-11 | 河南农业大学 | High-efficiency fungicide and method of preparing the same |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255801A (en) * | 2014-08-29 | 2015-01-07 | 浙江大洋生物科技集团股份有限公司 | Crop potassium supplement composition with sterilization function as well as preparation method and application of crop potassium supplement composition |
| CN104255801B (en) * | 2014-08-29 | 2016-06-29 | 浙江大洋生物科技集团股份有限公司 | The crop with bactericidal action mends potassium composition and its production and use |
| CN107027768A (en) * | 2017-04-28 | 2017-08-11 | 山东沃森农业科技有限公司 | A kind of bactericide for being used to prevent and treat dendrobium candidum southern blight |
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