CN114190386B - Compound composition of propamidine and sabinenol, bactericide and application - Google Patents

Compound composition of propamidine and sabinenol, bactericide and application Download PDF

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CN114190386B
CN114190386B CN202111451130.3A CN202111451130A CN114190386B CN 114190386 B CN114190386 B CN 114190386B CN 202111451130 A CN202111451130 A CN 202111451130A CN 114190386 B CN114190386 B CN 114190386B
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propamidine
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hinokitiol
agent
bactericide
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CN114190386A (en
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王勇
吴华
陈光友
蔡崇林
闫合
冯俊涛
马志卿
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Northwest A&F University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/52—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing groups, e.g. carboxylic acid amidines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/12—Powders or granules
    • A01N25/14—Powders or granules wettable
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

The invention provides a compound composition of propamidine and hinokitiol, a bactericide and application; the ratio by mass of propamidine: hinokitiol = (1-8): 2-1). The propamidine and the natural product sabinenol are compounded to prevent and treat sclerotinia rot of colza and gray mold of fruits and vegetables, and can be used for treating sclerotinia rot of colza and gray mold of fruits and vegetables which have drug resistance to benzimidazole and dicarboximide bactericides. The binary compound preparation has obvious synergy and can obviously reduce the use amount of the preparation for preventing and treating drug-resistant rape sclerotinia rot and fruit and vegetable gray mold, and the compound preparation is expected to be developed into a new generation of green agricultural bactericide.

Description

Compound composition of propamidine and sabinenol, bactericide and application
Technical Field
The invention belongs to the field of agricultural bactericides, and particularly relates to a compound composition of propamidine and hinokitiol, a bactericide and application. The two compounds are compounded to prevent and treat sclerotinia rot of colza, gray mold of fruits and vegetables and the method for treating sclerotinia rot of colza and gray mold of fruits and vegetables which have generated drug resistance to benzimidazole and dicarboximide bactericides, belonging to the invention of new application and application technology of the bactericides.
Background
Crop diseases cause enormous economic losses to agricultural production each year. At the present stage, chemical control is still an important means for controlling plant diseases. However, as chemical pesticides are repeatedly used for a long time, the problems of pathogenic bacteria resistance and pesticide residue are increasingly prominent. At present, people pay close attention to green food and environmental safety, and development of new environment-friendly green pesticides for preventing and treating crop diseases and insect pests is urgently needed. The pesticide can make up for the deficiencies of the pesticides, improve the control effect, reduce the cost and more importantly delay the occurrence of drug resistance. Propamidine (Propamidine) is an alkyl amidine environment-friendly compound, and is a novel bactericide variety developed and researched by Shanxi province biological pesticide engineering technical research center. Early researches show that propamidine has broad-spectrum antibacterial activity, has good inhibition effect on botrytis cinerea and sclerotinia sclerotiorum, does not have interactive drug resistance with the existing bactericide, and has wide application prospect.
Hinokitiol (hinokitiol) is a plant-derived natural product of tropolone family extracted from hinoki, and is a monoterpenoid compound having tropolone skeleton. Earlier researches find that the hinokitiol has wide biological activity, can be used as a natural plant source bactericide, and has excellent control effect on soil-borne diseases. The hinokitiol has simple structure, easy synthesis, environment friendship and high safety to non-target organism, is a natural, safe and efficient matter and has great potential for being developed into plant source bactericide.
Sclerotiniose (sclerotiostat rot) caused by the saprophytic fungus Sclerotinia sclerotiorum (lib.) de bark is a worldwide crop disease and can infect more than 400 plants of 278 genus of 75 families. The disease occurs in all rape planting areas in China, particularly in middle and lower reaches watershed of the coastal areas and the Yangtze river of southeast, the disease area is nearly 5000 ten thousand mu each year, the annual average yield loss is about 20 percent, and the annual average yield loss is up to 80 percent in serious conditions, even the disease is not harvested. After the rape is infected with the disease, the yield and the quality of the rapeseeds are seriously reduced, and the economic loss is hundreds of millions of yuan. Crop gray mold caused by Botrytis cinerea (Botrytis cinerea) is one of the ten major fungal diseases in the world. The pathogenic bacteria have wide host range, can infect more than 500 kinds of plants including various fruits, vegetables and flowers, and cause huge economic loss for agricultural production. The host of the botrytis cinerea can be attacked in each period from a seedling stage to a plant stage, conidiophores and conidia are generated on the surfaces of diseased parts by the pathogenic bacteria, and a gray mildew layer is usually formed on the surfaces of the diseased parts after the pathogenic bacteria are gradually accumulated. Benzimidazole fungicides such as carbendazim and benomyl, and dicarboximide fungicides such as dimethachlon and iprodione have been common agents for controlling the above two diseases for many years. However, the frequency and level of resistance of pathogenic bacteria to these agents has gradually increased due to the repeated use of large amounts of these agents. Such as Botrytis cinerea, have developed different levels of resistance to most agents registered on manufacture for the control of gray mold, and the problem of multiple resistance has become increasingly serious. Therefore, screening of novel efficient green alternative agents and compound agents is one of the important ways for preventing and treating the diseases.
Disclosure of Invention
The invention aims to provide binary compounding of propamidine and a natural product, namely hinokitiol, and can be simultaneously used for treating drug-resistant sclerotinia rot of colza and gray mold of fruits and vegetables.
The specific technical scheme is as follows:
a compound composition of propamidine and hinokitiol comprises the following components in percentage by mass: hinokitiol = (1-8): 2-1).
A compound composition of propamidine and hinokitiol comprises the following components in percentage by mass: hinokitiol =2:1.
A compound composition of propamidine and hinokitiol comprises the following components in percentage by mass: hinokitiol =4:1.
A compound composition of propamidine and hinokitiol comprises the following components in percentage by mass: hinokitiol =6:1.
The invention relates to application of a compound composition of propamidine and hinokitiol in preparation of a plant bactericide.
The invention discloses a propamidine and hinokitiol compound bactericide, which comprises the effective components of the propamidine and hinokitiol compound composition.
Optionally, the bactericide is an aqueous emulsion, and the weight percentage content is as follows:
1 to 40 percent of propamidine, 1 to 40 percent of hinokitiol, 5 to 10 percent of emulsifier, 0.5 to 2 percent of dispersant, 0.5 to 2 percent of antifreeze, 0.5 to 1 percent of preservative and 100 percent of solvent;
the dispersing agent is polyvinyl alcohol, arabic gum, xanthan gum or magnesium aluminum silicate;
the solvent is toluene, xylene, ethyl acetate or cyclohexanone;
the antifreezing agent is ethylene glycol, propylene glycol, glycerol or urea;
the preservative is sorbic acid, benzoic acid or benzaldehyde;
the emulsifier is one or a mixture of more than two of alkyl polyoxyethylene ether, fatty acid polyoxyethylene ester, phenethyl phenol polyoxyethylene ether, 7780, 7781T, 4103, OP-10, well201, no. 500, no. 602, well205 and M30.
Optionally, the bactericide is a suspending agent, and the weight percentage content is as follows:
1 to 50 percent of propamidine, 1 to 30 percent of cypress alcohol, 1 to 5 percent of wetting dispersant, 0.2 to 5 percent of defoamer, 0.5 to 5 percent of thickener, 0.5 to 5 percent of antioxidant, 0.5 to 2 percent of antifreeze and water to be supplemented to 100 percent;
the wetting dispersant is one or a mixture of more than two of alkylphenol resin polyvinyl ether, alkylbenzene sulfonate, long-chain fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether and fatty alcohol sulfate;
the defoaming agent is an acetylene glycol wetting defoaming agent or an organic silicon defoaming agent;
the thickening agent is polyvinyl alcohol, xanthan gum, sodium alginate, xanthan gum or hydroxypropyl methyl cellulose;
the antioxidant is ascorbic acid or amyl gallate;
the antifreezing agent is ethylene glycol, propylene glycol, glycerol or urea.
Optionally, the bactericide is water dispersible granules, and the weight percentage content is as follows:
1 to 40 percent of propamidine, 1 to 40 percent of hinokitiol, 1 to 5 percent of wetting agent, 1 to 5 percent of dispersing agent and 0.15 to 3 percent of disintegrating agent;
the raw materials of propamidine, sabinenol, wetting agent, dispersant and disintegrant are mixed uniformly, and then pulverized by a jet mill to prepare superfine wettable powder, then a certain amount of water is added and stirred uniformly, and the mixture is granulated by an extrusion granulator, dried by a jet dryer and sieved to obtain the water dispersible granule.
The propamidine and hinokitiol compound bactericide is applied to prevention and treatment of plant sclerotinia rot and/or gray mold.
Compared with the prior art, the invention has the advantages that:
(1) According to a series of proportioning researches, the invention discovers that when the propamidine and the hinokitiol are proportioned according to the mass ratio of (1-8) to (2-1), the synergistic coefficient is more than 1.5, and the propamidine and the hinokitiol have obvious synergistic effect.
(2) Greenhouse pot control experiments show that the bactericide prepared from propamidine and juniperitol has good control effects on sclerotinia rot and gray mold of carbendazim.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the present invention will be described in further detail with reference to embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The compound bactericide of propamidine and hinokitiol is a preparation compounded by propamidine and hinokitiol according to the mass ratio of (1-8) to (2-1), can prevent and control wild sensitive strains of sclerotinia sclerotiorum and botrytis cinerea of fruits and vegetables, and can prevent and control sclerotinia sclerotiorum and botrytis cinerea strains of fruits and vegetables which have drug resistance to benzimidazole and dicarboximide bactericides, and more importantly, the two bactericides have obvious synergistic effect under the above proportion and can reduce the dosage of chemical bactericides.
Dosage forms such as bactericides include aqueous emulsions, suspending agents and water dispersible granules;
the aqueous emulsion comprises the following components in percentage by weight: 1 to 40 percent of propamidine, 1 to 40 percent of hinokitiol, 5 to 10 percent of emulsifier, 0.5 to 2 percent of dispersant, 0.5 to 2 percent of antifreeze, 0.5 to 1 percent of preservative and 100 percent of solvent; the dispersant is polyvinyl alcohol, arabic gum, xanthan gum or magnesium aluminum silicate; the solvent is toluene, xylene, ethyl acetate or cyclohexanone; the antifreezing agent is ethylene glycol, propylene glycol, glycerol or urea; the antiseptic is sorbic acid, benzoic acid or benzaldehyde; the emulsifier is one or more of alkyl polyoxyethylene ether, fatty acid polyoxyethylene ester, phenethyl phenol polyoxyethylene ether, 7780, 7781T, 4103, OP-10, well201, no. 500, no. 602, well205 or M30.
The suspending agent comprises the following components in percentage by weight: 1 to 50 percent of propamidine, 1 to 30 percent of cypress alcohol, 1 to 5 percent of wetting dispersant, 0.2 to 5 percent of defoamer, 0.5 to 5 percent of thickener, 0.5 to 5 percent of ascorbic acid, 0.5 to 2 percent of antifreeze and water to 100 percent. The antioxidant is ascorbic acid or amyl gallate, and the wetting dispersant is one or more of alkylphenol resin polyvinyl ether, alkylbenzene sulfonate, long-chain fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether and fatty alcohol sulfate; the thickener is polyvinyl alcohol, xanthan gum, sodium alginate, xanthan gum or hydroxypropyl methyl cellulose; the defoaming agent is an acetylene glycol wetting defoaming agent and an organic silicon defoaming agent; the antifreezing agent is ethylene glycol, propylene glycol, glycerol or urea, etc.
The water dispersible granule comprises the following components in percentage by weight: 1-40% of propamidine, 1-40% of cypress wood alcohol, 1-5% of wetting agent, 1-5% of dispersing agent and 0.15-3% of disintegrating agent, wherein the raw materials of propamidine, cypress wood alcohol, wetting agent, dispersing agent and disintegrating agent are uniformly mixed, then are crushed by an airflow crusher to prepare superfine wettable powder, then a certain amount of water is added and uniformly stirred, and are granulated by an extrusion granulator, dried by an airflow dryer and sieved to obtain the water dispersible granule.
The propamidine and hinokitiol compounded synergistic bactericide is applied to the prevention and treatment of plant sclerotinia rot and gray mold, and is a substitute medicament for the drug resistance treatment of the sclerotinia rot and the gray mold.
Example 1: indoor in vitro determination of toxicity of propamidine and hinokitiol
The toxicity of propamidine and hinokitiol to pathogenic bacteria is determined by hypha growth rate method. Dissolving propamidine with methanol to 5000, 2500, 1250, 625, 312.5, 156.2 and 78.125ug/ml; dissolving hinokitiol in methanol to 25000, 12500, 6250, 3125, 1562.5, 781.25 and 390.625ug/ml; carbendazim was dissolved with 0.2M hydrochloric acid to 500, 250, 125, 62.5 and 31.25ug/ml. Sucking 0.2mL of the mother solution by using a pipette gun respectively, adding 100mL of PDA culture medium to prepare a drug-containing culture medium with final concentrations of 10, 5, 2.5, 1.25, 0.625 and 0.3125ug/mL propamidine respectively; medicated medium with final concentration of 50, 25, 12.5, 6.25, 3.125, 1.5625 and 0.78125ug/ml hinokitiol respectively; drug-containing media with final concentrations of 1, 0.5, 0.25, 0.125, and 0.0625ug/ml carbendazim, respectively. The cake of pathogenic bacteria with the diameter of 0.5cm at the edge of the colony was placed in the center of the dish (hypha facing down) and 0.2mL of methanol or 0.2M hydrochloric acid was added as a control. Culturing the inoculated culture dish at 25 deg.C for 3d, respectively measuring colony diameter by cross method, calculating bacteriostasis rate by using blank control as standard, and calculating effective inhibitory concentration (EC) by DPS software 50 ). The Sclerotinia sclerotiorum and Botrytis cinerea strains used in the experiment are both preserved in the laboratory. The pathogenic bacteria are separated from the diseased residues of the susceptible plants by adopting a conventional strain separation method and are obtained by a conventional strain identification method, and the specific pathogenic bacteria are numbered as the strains obtained by different isolates at different times, so that the special meaning is not generated.
Figure BDA0003386030180000051
TABLE 1 determination of sensitivity of carbendazim, propamidine, sabinenol to Sclerotium sclerotiorum
Figure BDA0003386030180000052
S is a multi-bacterium sensitive strain; r is carbendazim resistant strain.
As can be seen from Table 1, the propamidine, the hinokitiol and the carbendazim have no cross-drug resistance, and the propamidine and the hinokitiol have good bacteriostatic activity on sclerotinia sclerotiorum.
TABLE 2 determination of susceptibility of carbendazim, propamidine, and sabinenol to Botrytis cinerea
Figure BDA0003386030180000053
S is a multi-bacterium sensitive strain; r is carbendazim resistant strain.
As can be seen from Table 2, the propamidine, the cypress wood alcohol and the carbendazim do not have cross drug resistance, and the propamidine and the cypress wood alcohol have good bacteriostatic activity on gray mold of fruits and vegetables.
Example 2: synergistic effect of propamidine and sabinenol in different proportions
Respectively measuring the toxicity of propamidine and hinokitiol to pathogenic bacteria at different ratios by using a hypha growth rate reference method
The fungus cake is transferred to a PDA plate containing a series of compound medicines with concentration, and is cultured at 25 ℃ for 2 constant temperature culture. Measuring the diameters of all colonies when the diameters of the colonies in the blank control group exceed 2/3 of the diameter of the whole culture dish, repeating the treatment for 3 times, and calculating the hypha growth inhibition rate and the effective inhibition medium concentration (EC) 50 ) The synergistic coefficient of the mixed medicament is calculated according to the following formula. When the synergistic coefficient is more than 1.5, the synergistic effect is achieved; the additive effect is between 0.5 and 1.5; less than 0.5 is antagonistic (a and b are the ratio of propamidine and hinokitiol in the composition, EC 50 (A) And EC 50 (B) Is an effective medium concentration of propamidine and hinokitiol, EC 50 (Exp) is the theoretical inhibitory median concentration, EC 50 (Obs) is the actual concentration in inhibition measured).
Figure BDA0003386030180000061
TABLE 3 virulence determination of the different proportions of propamidine and sabinenol against the Sclerotium sclerotiorum carbendazim resistant strain JK16R
Figure BDA0003386030180000062
The data in Table 3 show that the toxicity of propamidine and hinokitiol to sclerotinia sclerotiorum is different, the synergy coefficient value is 0.9558-2.0774, the synergy coefficients of the proportions are all larger than 0.5, the additive effect is shown in the proportions, particularly the synergy coefficients are all larger than 1.5 when the mass ratio of propamidine to hinokitiol is 1:2-8:1, and the propamidine and hinokitiol have obvious synergy to sclerotinia sclerotiorum in the proportions.
TABLE 4 virulence of Propamidine and Hinokitiol against Botrytis cinerea carbendazim resistant strain NJ4-1R in different ratios
Figure BDA0003386030180000071
The data in table 4 show that the ratio of propamidine to hinokitiol is 1: 10-10, the synergistic coefficients are all more than 0.5 between 0.6317-2.3037, which shows that the synergistic compounds have additive effect under the above mixture ratio, especially the synergistic coefficients are all more than 1.5 when the mixture ratio is 1:1-8:1, which shows that the synergistic compounds have significant synergistic effect under the above mixture ratio. The propamidine and the hinokitiol in the proportion have obvious synergistic effect on preventing and treating gray mold of fruits and vegetables under the combined action.
Example 3: production of 15% propamidine and 15% hinokitiol composition emulsion in water A
167 kg of 90% propamidine raw material and 160 kg of 94% hinokitiol raw material are weighed accurately and quantitatively. Mixing the two active components in 2M 3 Mixing in blending tank. Accurately weighing 10 kg of ascorbic acid, putting into a blending tank, and fully and uniformly mixing again. Then 80 kg of emulsifier Well201, 10 kg of Arabic gum, 10 kg of urea, 10 kg of sorbic acid and 500 kg of ethyl acetate are accurately put into a blending tank, and the mixture is added until the mixture is completely filled to 1000 kg, and is fully and uniformly mixed in the blending tank. Stirring was continued for 1 hour. 1 ton of propamidine and hinokitiol composition in an aqueous emulsion is prepared.
The content of propamidine and hinokitiol was 15% by sampling analysis. And (3) subpackaging the product into aluminum cans coated with barrier materials, wherein each can contains 5 kg of the product, and the total amount of the product is 200 cans. Sealing the opening of the tank, and sticking a label to obtain the composition product A aqueous emulsion product with the content of 30 percent.
Example 4: production of 30% propamidine and 15% hinokitiol composition as suspending agent B
333 kilograms of 90 percent propamidine raw material, 160 kilograms of 94 percent hinokitiol raw material, 10 kilograms of ascorbic acid, 205 kilograms of emulsifier Well, 40 kilograms of wetting dispersant long-chain fatty alcohol-polyoxyethylene ether, 30 kilograms of thickening agent polyvinyl alcohol and 25 kilograms of defoaming agent organic silicon are weighed accurately and quantitatively, put into a blending tank and stirred fully and uniformly in the blending tank. Then quantitatively adding 100 kg of agricultural emulsifier TX-10 into the blending tank, adding the mixture to 1000 kg, stirring while adding, and after all the materials are added, continuing stirring for 1 hour by using a stirrer.
The content of propamidine in the sampled test product is 30 percent, the content of hinokitiol in the sampled test product is 15 percent, and 1 ton of the product with the total content of effective components of 45 percent is finally obtained, and the bottle mouth of the product is sealed by a 500mL brown plastic bottle.
Example 5: production of propamidine and hinokitiol 4:1 composition water dispersible granule C
The embodiment provides a formula of a propamidine and juniperitol 4:1 compound water dispersible granule, and the prepared compound medicament water dispersible granule consists of the following raw materials in percentage by mass: 40 to 60 percent of propamidine, 10 to 15 percent of hinokitiol, 2.5 to 5 percent of dispersant, 2.5 to 5 percent of wetting agent, 2.5 to 5 percent of disintegrant and 5 to 10 percent of filler, wherein the sum of the mass percentages of the raw materials is 100 percent.
The propamidine technical, the hinokitiol technical, the wetting agent, the dispersing agent, the disintegrating agent and the filler are uniformly mixed and then crushed by a jet mill to prepare superfine wettable powder, then a certain amount of water is added, granulation is carried out by an extrusion granulator, and the granules are dried by air flow and screened to obtain the water dispersible granules.
Example 6: compound agent of propamidine and sabinenol for preventing sclerotinia rot of rape and gray mold of fruit and vegetable
The prevention and treatment effects of the compound pesticide on sclerotinia rot of colza and gray mold of fruits and vegetables are determined by adopting a greenhouse pot experiment. When the seedlings grow to 3-5 leaves, the test is carried out. The tested agents are the prepared emulsion in water A, a suspending agent B, a water dispersible granule C, propamidine and hinokitiol. Carbendazim (50% wp) was a positive control. Each treatment was repeated for 3 pots. And (4) inoculating carbendazim resistant pathogenic bacteria and then applying the pesticide by using conventional spraying. The treated seedlings were cultivated in a greenhouse. After culturing for 3-5 days, measuring the diameter of the disease spot, and calculating the prevention and treatment effect according to the following formula, wherein the measurement results are shown in tables 5 and 6.
TABLE 5 propamidine and sabinenol combinations for combating sclerotinia rot of colza against carbendazim
Figure BDA0003386030180000081
Figure BDA0003386030180000091
Note: values in the same column are followed by different letters indicating significant differences between the values (P = 0.05) as determined by Fisher's least significant differences. Control effect (%) = (control lesion diameter-treatment lesion diameter)/(control lesion diameter-0.5) × 100.
As can be seen from Table 5, greenhouse pot experiments prove that the synergistic effect of the two agents of the propamidine and the sabinol on preventing and treating the sclerotinia rot of colza is obvious. The control effect of different formulations of the propamidine and hinokitiol compound medicament on sclerotinia rot of colza is higher than that of each single agent when the single agent is used independently, and is obviously higher than that of a contrast medicament carbendazim.
TABLE 6 propamidine and hinokitiol combinations against carbendazim-resistant grey mould of fruits and vegetables
Figure BDA0003386030180000092
Note: values in the same column are followed by different letters indicating significant differences between the values (P = 0.05) as determined by Fisher's least significant differences. Control effect (%) = (control lesion diameter-treatment lesion diameter)/(control lesion diameter-0.5) × 100.
As can be seen from Table 6, greenhouse pot experiments prove that the synergistic effect of the propamidine and the sabinol on preventing and controlling the gray mold of the fruits and vegetables is obvious when the propamidine and the sabinol are compounded. The control effect of different formulations of the propamidine and hinokitiol compound medicament on the gray mold of fruits and vegetables is higher than that of a contrast medicament carbendazim and each single agent.
Although the invention has been described in detail with respect to the general description and the specific embodiments thereof, it will be apparent to those skilled in the art that modifications and improvements can be made based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims (10)

1. The compound composition of propamidine and hinokitiol is characterized in that the propamidine comprises the following components in percentage by mass: hinokitiol = (1-8): 2-1).
2. The compound composition of propamidine and hinokitiol is characterized in that the propamidine comprises the following components in percentage by mass: hinokitiol =2:1.
3. The compound composition of propamidine and hinokitiol is characterized in that the propamidine comprises the following components in percentage by mass: hinokitiol =4:1.
4. The compound composition of propamidine and hinokitiol is characterized in that the propamidine comprises the following components in percentage by mass: hinokitiol =6:1.
5. Use of a combination of a propamidine and hinokitiol according to any one of claims 1 to 4 for the preparation of a plant fungicide.
6. The compound bactericide of propamidine and hinokitiol is characterized in that the active ingredient of the bactericide is the compound composition of propamidine and hinokitiol in any one of claims 1 to 4.
7. The propamidine and hinokitiol compound bactericide as claimed in claim 6, wherein the bactericide is an aqueous emulsion, and comprises the following components in percentage by weight:
1 to 40 percent of propamidine, 1 to 40 percent of cypress alcohol, 5 to 10 percent of emulsifier, 0.5 to 2 percent of dispersant, 0.5 to 2 percent of antifreeze, 0.5 to 1 percent of preservative and 100 percent of solvent;
the dispersing agent is polyvinyl alcohol, arabic gum, xanthan gum or magnesium aluminum silicate;
the solvent is toluene, xylene, ethyl acetate or cyclohexanone;
the antifreezing agent is ethylene glycol, propylene glycol, glycerol or urea;
the preservative is sorbic acid, benzoic acid or benzaldehyde;
the emulsifier is one or a mixture of more than two of alkyl polyoxyethylene ether, fatty acid polyoxyethylene ester and phenethyl phenol polyoxyethylene ether.
8. The propamidine and hinokitiol compound bactericide as claimed in claim 6, wherein the bactericide is a suspending agent, and comprises the following components in percentage by weight:
1 to 50 percent of propamidine, 1 to 30 percent of hinokitiol, 1 to 5 percent of wetting dispersant, 0.2 to 5 percent of defoaming agent, 0.5 to 5 percent of thickening agent, 0.5 to 5 percent of antioxidant, 0.5 to 2 percent of antifreezing agent and water to be supplemented to 100 percent;
the wetting dispersant is one or a mixture of more than two of alkylphenol resin polyvinyl ether, alkylbenzene sulfonate, long-chain fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether and fatty alcohol sulfate;
the defoaming agent is an acetylene glycol wetting defoaming agent or an organic silicon defoaming agent;
the thickening agent is polyvinyl alcohol, xanthan gum, sodium alginate, xanthan gum or hydroxypropyl methyl cellulose;
the antioxidant is ascorbic acid or amyl gallate;
the antifreezing agent is ethylene glycol, propylene glycol, glycerol or urea.
9. The propamidine and hinokitiol compound bactericide as claimed in claim 6, wherein the bactericide is a water dispersible granule, and comprises the following components in percentage by weight:
1 to 40 percent of propamidine, 1 to 40 percent of hinokitiol, 1 to 5 percent of wetting agent, 1 to 5 percent of dispersing agent and 0.15 to 3 percent of disintegrating agent;
the raw materials of propamidine, sabinenol, wetting agent, dispersant and disintegrant are mixed uniformly, and then pulverized by a jet mill to prepare superfine wettable powder, then a certain amount of water is added and stirred uniformly, and the mixture is granulated by an extrusion granulator, dried by a jet dryer and sieved to obtain the water dispersible granule.
10. The use of a propamidine in combination with hinokitiol as claimed in any one of claims 6 to 9 as a fungicide for the control of sclerotinia rot and/or gray mold in plants.
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