CN102272334B - 新基因组测序策略 - Google Patents

新基因组测序策略 Download PDF

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Publication number
CN102272334B
CN102272334B CN201080004824.6A CN201080004824A CN102272334B CN 102272334 B CN102272334 B CN 102272334B CN 201080004824 A CN201080004824 A CN 201080004824A CN 102272334 B CN102272334 B CN 102272334B
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sequence
fragment
joint
contig
dna
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CN201080004824.6A
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English (en)
Chinese (zh)
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CN102272334A (zh
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M·J·T·范艾杰克
A·J·范托恩
A·A·A·W·扬森
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Keygene NV
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Keygene NV
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201080004824.6A 2009-01-13 2010-01-13 新基因组测序策略 Expired - Fee Related CN102272334B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US14428109P 2009-01-13 2009-01-13
US61/144,281 2009-01-13
US22646809P 2009-07-17 2009-07-17
US61/226,468 2009-07-17
NL2003235 2009-07-17
NL2003235 2009-07-17
PCT/NL2010/000003 WO2010082815A1 (en) 2009-01-13 2010-01-13 Novel genome sequencing strategies

Publications (2)

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CN102272334A CN102272334A (zh) 2011-12-07
CN102272334B true CN102272334B (zh) 2014-08-20

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CN201080004824.6A Expired - Fee Related CN102272334B (zh) 2009-01-13 2010-01-13 新基因组测序策略

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US (1) US20130196859A1 (enExample)
EP (1) EP2379751B1 (enExample)
JP (1) JP2012514977A (enExample)
CN (1) CN102272334B (enExample)
ES (1) ES2403312T3 (enExample)
WO (1) WO2010082815A1 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011074960A1 (en) * 2009-12-17 2011-06-23 Keygene N.V. Restriction enzyme based whole genome sequencing
FR2968671B1 (fr) * 2010-12-13 2015-05-01 Chaouki Miled Sequencage maspet
WO2013152505A1 (zh) * 2012-04-13 2013-10-17 深圳华大基因科技服务有限公司 一种转录组组装的方法及系统
EP4036247B1 (en) * 2012-09-04 2024-04-10 Guardant Health, Inc. Methods to detect rare mutations and copy number variation
CA2884246C (en) * 2012-09-24 2023-11-07 Cb Biotechnologies, Inc. Multiplex pyrosequencing using non-interfering noise-canceling polynucleotide identification tags
CN102982252A (zh) * 2012-12-05 2013-03-20 北京诺禾致源生物信息科技有限公司 一种高杂合二倍体基因组支架序列组装策略
MX364309B (es) * 2013-04-17 2019-04-22 Pioneer Hi Bred Int Métodos para caracterizar la composición de secuencias de adn en un genoma.
CN103388025B (zh) * 2013-07-10 2015-04-29 华中农业大学 基于克隆dna混合池的全基因组测序方法
CN111863131A (zh) 2013-10-07 2020-10-30 塞昆纳姆股份有限公司 用于非侵入性评估染色体改变的方法和过程
JP6418800B2 (ja) * 2014-06-17 2018-11-07 国立大学法人 東京大学 外来性dnaのゲノムへの導入位置の決定法
US9710296B2 (en) 2014-12-09 2017-07-18 Vmware, Inc. Allocating cost of disk usage to a linked clone virtual machine
US9582309B2 (en) 2014-12-09 2017-02-28 Vmware, Inc. Allocating cost of disk usage to a linked clone virtual machine based on a parameter of usage
WO2016134034A1 (en) * 2015-02-17 2016-08-25 Dovetail Genomics Llc Nucleic acid sequence assembly
CN106021978B (zh) * 2016-04-06 2019-03-29 晶能生物技术(上海)有限公司 基于光学图谱平台Irys的一种de novo测序数据组装方法
CN106778060B (zh) * 2016-10-09 2019-05-21 南京双运生物技术有限公司 一种利用原核生物基因组高质量草图制作完成图的方法
CN108660197A (zh) * 2017-04-01 2018-10-16 深圳华大基因科技服务有限公司 一种二代序列基因组重叠群的组装方法和系统

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008007951A1 (en) * 2006-07-12 2008-01-17 Keygene N.V. High throughput physical mapping using aflp

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0534858B2 (en) 1991-09-24 2005-04-27 Keygene N.V. Selective restriction fragment amplification : a general method for DNA fingerprinting
DE69928265T3 (de) 1998-07-30 2013-11-28 Illumina Cambridge Ltd. Matrizen von biomolekülen und ihre verwendung in sequenzierung
WO2002061126A2 (en) 2001-01-30 2002-08-08 Solexa Ltd. The preparation of polynucleotide arrays
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
EP1124990B1 (en) 1998-10-27 2006-01-18 Affymetrix, Inc. Complexity management and analysis of genomic dna
CA2348696A1 (en) 1998-11-06 2000-05-18 Solexa Ltd. A method for reproducing molecular arrays
WO2000058507A1 (en) 1999-03-30 2000-10-05 Solexa Ltd. Polynucleotide sequencing
EP1218543A2 (en) 1999-09-29 2002-07-03 Solexa Ltd. Polynucleotide sequencing
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
US7809509B2 (en) 2001-05-08 2010-10-05 Ip Genesis, Inc. Comparative mapping and assembly of nucleic acid sequences
US20030096268A1 (en) 2001-07-06 2003-05-22 Michael Weiner Method for isolation of independent, parallel chemical micro-reactions using a porous filter
GB0119719D0 (en) 2001-08-13 2001-10-03 Solexa Ltd DNA sequence analysis
US6975943B2 (en) 2001-09-24 2005-12-13 Seqwright, Inc. Clone-array pooled shotgun strategy for nucleic acid sequencing
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
ES2550513T3 (es) 2002-08-23 2015-11-10 Illumina Cambridge Limited Nucleótidos modificados para secuenciación de polinucleótidos
EP3438116B1 (en) 2002-08-23 2021-02-17 Illumina Cambridge Limited Labelled nucleotides
US20060094016A1 (en) 2002-12-02 2006-05-04 Niall Gormley Determination of methylation of nucleic acid sequences
EP1594950A4 (en) 2003-01-29 2007-07-25 454 Corp PRODUCT FOR THE PREPARATION OF SIMPLE STRANDED DNA BANKS
GB0304371D0 (en) 2003-02-26 2003-04-02 Solexa Ltd DNA Sequence analysis
GB0320059D0 (en) 2003-08-27 2003-10-01 Solexa Ltd A method of sequencing
GB0326073D0 (en) 2003-11-07 2003-12-10 Solexa Ltd Improvements in or relating to polynucleotide arrays
EP2789383B1 (en) 2004-01-07 2023-05-03 Illumina Cambridge Limited Molecular arrays
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
GB0400974D0 (en) 2004-01-16 2004-02-18 Solexa Ltd Multiple inexact matching
GB0402895D0 (en) 2004-02-10 2004-03-17 Solexa Ltd Arrayed polynucleotides
ATE465274T1 (de) 2005-06-23 2010-05-15 Keygene Nv Verbesserte strategien zur sequenzierung komplexer genome unter verwendung von sequenziertechniken mit hohem durchsatz
US20070082358A1 (en) 2005-10-11 2007-04-12 Roderic Fuerst Sequencing by synthesis based ordered restriction mapping
WO2010036287A1 (en) * 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008007951A1 (en) * 2006-07-12 2008-01-17 Keygene N.V. High throughput physical mapping using aflp

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Shotgun sequencing, clone pooling, and comparative strategies for mapping and sequencing;Aleksandar Milosavljevic;《TARGETS》;20031231;第12卷(第6期);246-252 *
The Genome of the Sea Urchin Strongylocentrotus purpuratus;Sea Urchin Genome Sequencing Consortium;《SCIENCE》;20061110;第314卷;942 *

Also Published As

Publication number Publication date
WO2010082815A1 (en) 2010-07-22
EP2379751A1 (en) 2011-10-26
EP2379751B1 (en) 2013-03-20
JP2012514977A (ja) 2012-07-05
ES2403312T3 (es) 2013-05-17
US20130196859A1 (en) 2013-08-01
CN102272334A (zh) 2011-12-07

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