CN102265798A - Sepia cuttlebone tetracycline labeling method and special reagents thereof - Google Patents
Sepia cuttlebone tetracycline labeling method and special reagents thereof Download PDFInfo
- Publication number
- CN102265798A CN102265798A CN2011101735001A CN201110173500A CN102265798A CN 102265798 A CN102265798 A CN 102265798A CN 2011101735001 A CN2011101735001 A CN 2011101735001A CN 201110173500 A CN201110173500 A CN 201110173500A CN 102265798 A CN102265798 A CN 102265798A
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- China
- Prior art keywords
- tetracycline
- water body
- casing
- cuttlebone
- cuttlefish
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000004098 Tetracycline Substances 0.000 title claims abstract description 48
- 229960002180 tetracycline Drugs 0.000 title claims abstract description 48
- 229930101283 tetracycline Natural products 0.000 title claims abstract description 48
- 235000019364 tetracycline Nutrition 0.000 title claims abstract description 48
- 150000003522 tetracyclines Chemical class 0.000 title claims abstract description 48
- 238000002372 labelling Methods 0.000 title abstract description 11
- 239000003153 chemical reaction reagent Substances 0.000 title abstract description 5
- 241000238370 Sepia Species 0.000 title abstract 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 46
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 14
- 238000009395 breeding Methods 0.000 claims description 48
- 230000001488 breeding effect Effects 0.000 claims description 48
- 238000009792 diffusion process Methods 0.000 claims description 30
- 241000238371 Sepiidae Species 0.000 claims description 28
- 239000003550 marker Substances 0.000 claims description 16
- 238000007790 scraping Methods 0.000 claims description 10
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 230000008520 organization Effects 0.000 claims description 6
- 239000013535 sea water Substances 0.000 claims description 4
- 238000002203 pretreatment Methods 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- 241001663378 Sepiella maindroni Species 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 5
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 abstract description 3
- 229910001424 calcium ion Inorganic materials 0.000 abstract description 3
- 230000002452 interceptive effect Effects 0.000 abstract description 3
- 150000002500 ions Chemical class 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 238000012544 monitoring process Methods 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 230000001066 destructive effect Effects 0.000 abstract 2
- 238000011017 operating method Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 208000035874 Excoriation Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
Images
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a sepia cuttlebone tetracycline labeling method and special reagents thereof, and the sepia cuttlebone tetracycline labeling method comprises the following operation step: placing sepia which needs to be labeled into a cultivation water body with tetracycline for performing cultivation, wherein the concentration of the tetracycline in the cultivation water body is 100-200mg/T; and 1-5g of EDTA (ethylenediaminetetraacetic acid) is firstly added into each ton of the cultivation water body for pretreatment before the tetracycline is dissolved in the cultivation water body. The corresponding reagents comprise 0.1-0.2 part by weight of the tetracycline and 1-5 parts of the EDTA. By adopting the method and the reagents, calcium ions in the cultivation water body can be removed, the loss of the tetracycline caused by interfering ions in the follow-up labeling step can be avoided, and the stable labeling concentration, the stable labeling effect, the non-destructive labeling of the sepia and small stress reaction can be further ensured. Furthermore, the sepia cuttlebone tetracycline labeling method is low in implementation cost, convenient to operate and high in operation efficiency, can be used for further realizing the growth monitoring and non-destructive chemical labeling of sepiella maindroni and further has the advantages of convenience in observation and low cost.
Description
Technical field
The present invention relates to the harmless labeling method of a kind of aquatic animal and special marker agent prescription, particularly cuttlefish cuttlebone tetracycline marker method and special agent thereof.
Background technology
Sepiella maindroni once was one of the four big fishery in China fishing ground, and was stronger to the adaptability of temperature, in China coastal distribution very wide, be the very high traditional fishery kind of a kind of economic worth.Because overfishing and ecological degeneration since the seventies in 20th century, decay successively occurred.Last century, the eighties had the Primary Study to Sepiella maindroni resource and propagation such as report Li Xing a word used in person's names, and abroad had the biology of merchant cuttlefish and the report of breed aspect.Cuttlefish is fished for pressure owing to be subjected to fishery for a long time, and population quantity falls sharply, and needs badly by manually breeding and releases.After progressively breaking through the cuttlefish raising technology, release and put on agenda.The related scientific research personnel of Oceanography Institute Of Zhejiang repeatedly Sepiella maindroni are carried out artificial breeding and propagation is released, but find in practice for upgrowth situation to release effect and further further investigation cuttlefish, need development of new reality that cuttlefish is carried out corresponding mark, satisfy the needs of research and production.
Summary of the invention
The purpose of this invention is to provide cuttlefish cuttlebone tetracycline marker method and special agent thereof, realize the growth monitoring of Sepiella maindroni is reached harmless chemical labeling with this, and have convenient, the with low cost advantage of observation.
The present invention is achieved in that cuttlefish cuttlebone tetracycline marker method, comprises the cuttlefish that needs mark is put into the operating procedure that breeding water body with tetracycline is cultured; Tetracycline concentration in the breeding water body is 100 to 200mg/T; Breeding water body added EDTA earlier in breeding water body per ton before the dissolving tetracycline be 1 to 5g preliminary treatment.
Cuttlefish cuttlebone tetracycline marker method has following steps:
1. pre-treatment step: will put into slowly-releasing mark breeding box and little inflation through the seawater that purifies, as breeding water body; Dissolve in EDTA in the breeding water body and transfer concentration to 1～5g/T, fully the dissolving back is stand-by.
2. adapt to step: cuttlefish to be marked is put into a side breeding water body of the diffusion adjusting device of slowly-releasing mark breeding box, and keep the little inflation of this part water body;
3. markers step:, put into the opposite side of the diffusion adjusting device of slowly-releasing mark breeding box with the ratio of tetracycline with 100～200mg/T breeding water body.
Slowly-releasing mark breeding box has casing, and is equipped with in the casing and the inside of casing can be divided into two-part diffusion adjusting device with folding function; The DIFFUSION CONTROLLED part that can control the folding of diffusion adjusting device also is installed in the casing.
The diffusion adjusting device is at least three active clapboards that the gap is provided with; Two end evaginations at an edge of active clapboard and connect box movingly; The edge of the movable part of active clapboard is shaped on the draw-in groove of indent; Draw-in groove is connected with flexible scraping blade, and flexible scraping blade is slidingly matched near the part of the cabinet wall inwall with casing; Bottom half is shaped on and can limits the limiting plate that the dividing plate rotation is crossed the border.
The DIFFUSION CONTROLLED part contains and runs through the connecting rod that is connected casing top; Connecting rod is provided with unidirectional stepping controlling organization; Be connected with rope on the connecting rod; Rope runs through active clapboard successively, and the gap is provided with the card ball on the rope; Unidirectional stepping controlling organization is the ratchet that is arranged on connecting rod one side; The casing outside is provided with the grab with spring answer part near the part of ratchet.
The corresponding cuttlefish cuttlebone tetracycline marker special agent that provides, it contains 0.1 to 0.2 part tetracycline, 1 to 5 part EDTA by weight.
Compared with prior art, the invention has the advantages that the operating procedure that breeding water body that the present invention has used the cuttlefish that will need mark to put into to have tetracycline is cultured; Tetracycline concentration in the breeding water body is 100 to 200mg/T; Breeding water body added EDTA earlier in breeding water body per ton before the dissolving tetracycline be 1 to 5g preliminary treatment.Method of the present invention and reagent can be removed the calcium ion in the breeding water body, avoid then the employed tetracycline of markers step owing to the loss that interfering ion causes, guarantee the stable and mark effect of label concentration stable, to realizing that cuttlefish can't harm mark, stress reaction is little.And that the present invention realizes is with low cost, easy to operate, operating efficiency is high.
Description of drawings
Fig. 1 is an embodiment of the invention slowly-releasing mark breeding box perspective view;
Fig. 2 is an embodiment of the invention slowly-releasing mark breeding box sectional structure schematic diagram;
Fig. 3 is the local parts stereo amplification figure of embodiment of the invention slowly-releasing mark breeding box;
Fig. 4 is an embodiment of the invention flow chart.
Embodiment
Below in conjunction with attached embodiment the present invention is described in further detail.
Drawing reference numeral explanation: casing 1, limiting plate 11, diffusion adjusting device 2, active clapboard 21, draw-in groove 21a, flexible scraping blade 22, DIFFUSION CONTROLLED part 3, connecting rod 31, ratchet 32, grab 33, rope 34, card ball 35.
Embodiment: referring to figs. 1 through Fig. 4, cuttlefish cuttlebone tetracycline marker method comprises the cuttlefish that needs mark is put into the operating procedure that breeding water body with tetracycline is cultured; Tetracycline concentration in the breeding water body is 100 to 200mg/T; Breeding water body added EDTA earlier in breeding water body per ton before the dissolving tetracycline be 1 to 5g preliminary treatment.
Cuttlefish cuttlebone tetracycline marker method has following steps:
1. pre-treatment step: will put into slowly-releasing mark breeding box and little inflation through the seawater that purifies, as breeding water body; Dissolve in EDTA in the breeding water body and transfer concentration to 1～5g/T, fully the dissolving back is stand-by.Prevent that with the shielding heavy metal of aquaculture common usage the purpose of paedomorphosis deformity is different, the purpose that EDTA of the present invention uses is the calcium ion of removing in the breeding water body, the loss of avoiding the employed tetracycline of markers step then to cause owing to interfering ion.The preferred 5gEDTA of present embodiment, breeding water body are 1 ton of seawater.
2. adapt to step: cuttlefish to be marked is put into a side breeding water body of the diffusion adjusting device 2 of slowly-releasing mark breeding box, and keep the little inflation of this part water body; The aerating device that the little inflation of water body is adopted adopts and is positioned at the bigger side of diffusion adjusting device 2 water bodys.
3. markers step:, put into the opposite side of the diffusion adjusting device 2 of slowly-releasing mark breeding box with the ratio of tetracycline with 100～200mg/T breeding water body.What diffusion adjusting device 2 water bodys were less one is sidelong and puts tetracycline.Cause the phenomenon of concentration deficiency in order to prevent that tetracycline or EDTA are floating, can consider to adopt the bolting silk parcel.The preferred 0.1g tetracycline of present embodiment.
Slowly-releasing mark breeding box has casing 1, and is equipped with in the casing 1 and the inside of casing 1 can be divided into two-part diffusion adjusting device 2 with folding function; The DIFFUSION CONTROLLED part 3 that can control 2 foldings of diffusion adjusting device also is installed in the casing 1.With a side of test drug input diffusion adjusting device 2, diffusion naturally by water body prevent test organism stress, and shortening experimental period.The control that DIFFUSION CONTROLLED part 3 is realized natural diffusion velocity indirectly by control diffusion adjusting device 2.
At least three active clapboards 21 that diffusion adjusting device 2 is provided with for the gap; Two end evaginations at an edge of active clapboard 21 and connect box 1 movingly; The edge of the movable part of active clapboard 21 is shaped on the draw-in groove 21a of indent; Draw-in groove 21a is connected with flexible scraping blade 22, and flexible scraping blade 22 is slidingly matched near the part of the casing 1 inwalls inwall with casing 1; Casing 1 bottom is shaped on can limit the limiting plate 11 that dividing plate 21 rotations are crossed the border.The rotation of dividing plate 21 forms the size in continuous part gap, two water body zones, is spaced from each other fully until approximate.Manually limit the medicine that is arranged in active clapboard 21 1 side water bodys in mode comparatively easily and freely be diffused into the opposite side water body.The flexible scraping blade 22 in side that is slidingly matched with casing 1 inwall can play airtight effect, with 22 of partially flexible scraping blades that rotate axially parallel is to play sealing function under the vertical state of active clapboard 21, adopts the flexible scraping blade 22 of flexible material can prevent the skin abrasion of experimental animal simultaneously.
DIFFUSION CONTROLLED part 3 contains and runs through the connecting rod 31 that is connected casing 1 top; Connecting rod 31 is provided with unidirectional stepping controlling organization; Be connected with rope 34 on the connecting rod 31; Rope 34 runs through active clapboard 21 successively, and the gap is provided with card ball 35 on the rope 34; Unidirectional stepping controlling organization is the ratchet 32 that is arranged on connecting rod 31 1 sides; Casing 1 outside is provided with the grab 33 with spring answer part near the part of ratchet 32.The card ball 35 that is fixed on the rope 34 can be adjusted the angle that active clapboard 21 tilts under the drive of rope 34 tractions.Ratchet 32 and grab 33 are replied under the effect of part at spring, can limit rope 34 at active clapboard 21 action of gravity drag ropes 34, and then make active clapboard 21 be stabilized in certain angle of inclination.Release catch 33 can be put down active clapboard 21 and oppositely regulate.
Cuttlefish cuttlebone tetracycline marker special agent, it contains 0.1 to 0.2 part tetracycline, 1 to 5 part EDTA by weight.The preferred 0.1g tetracycline of present embodiment is joined 5gEDTA.
Although described the present invention in conjunction with preferred embodiment; right its is not in order to limit the present invention; any those skilled in the art; under the situation that does not break away from the spirit and scope of the present invention; can implement the displacement and the modification of various changes, coordinate to the theme of listing here, so protection scope of the present invention is as the criterion when looking the claim restricted portion that is proposed.
Claims (6)
1. cuttlefish cuttlebone tetracycline marker method is characterized in that: will need the cuttlefish of mark to put into the breeding water body with tetracycline and culture; Tetracycline concentration in the described breeding water body is 100 to 200mg/T; Described breeding water body added EDTA earlier in breeding water body per ton before the dissolving tetracycline be 1 to 5g preliminary treatment.
2. cuttlefish cuttlebone tetracycline marker method according to claim 1 is characterized in that having following steps:
1. pre-treatment step: will put into slowly-releasing mark breeding box and little inflation through the seawater that purifies, as breeding water body; Dissolve in EDTA in the described breeding water body and transfer concentration to 1～5g/T, fully the dissolving back is stand-by;
2. adapt to step: cuttlefish to be marked is put into a side breeding water body of the diffusion adjusting device (2) of described slowly-releasing mark breeding box, and keep the little inflation of this part water body;
3. markers step:, put into the opposite side of the diffusion adjusting device (2) of described slowly-releasing mark breeding box with the ratio of tetracycline with 100～200mg/T breeding water body.
3. cuttlefish cuttlebone tetracycline marker method according to claim 2, it is characterized in that: described slowly-releasing mark breeding box has casing (1), and is equipped with in the described casing (1) and the inside of casing (1) can be divided into two-part diffusion adjusting device (2) with folding function; The DIFFUSION CONTROLLED part (3) that can control described diffusion adjusting device (2) folding also is installed in the described casing (1).
4. cuttlefish cuttlebone tetracycline marker method according to claim 3 is characterized in that: at least three active clapboards (21) that described diffusion adjusting device (2) is provided with for the gap; Two end evaginations at an edge of described active clapboard (21) also connect described casing (1) movingly; The edge of the movable part of described active clapboard (21) is shaped on the draw-in groove (21a) of indent; Described draw-in groove (21a) is connected with flexible scraping blade (22), and described flexible scraping blade (22) is slidingly matched near the part of casing (1) the inwall inwall with described casing (1); Described casing (1) bottom is shaped on can limit the limiting plate (11) that described dividing plate (21) rotation is crossed the border.
5. cuttlefish cuttlebone tetracycline marker method according to claim 3 is characterized in that: described DIFFUSION CONTROLLED part (3) contains and runs through the connecting rod (31) that is connected described casing (1) top; Described connecting rod (31) is provided with unidirectional stepping controlling organization; Be connected with rope (34) on the described connecting rod (31); Described rope (34) runs through described active clapboard (21) successively, and rope (34) upward is provided with card ball (35) in the gap; Described unidirectional stepping controlling organization is for being arranged on the ratchet (32) of described connecting rod (31) one sides; Described casing (1) outside is provided with the grab (33) with spring answer part near the part of ratchet (32).
6. cuttlefish cuttlebone tetracycline marker special agent is characterized in that: contain 0.1 to 0.2 part tetracycline, 1 to 5 part EDTA by weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101735001A CN102265798B (en) | 2011-06-24 | 2011-06-24 | Sepia cuttlebone tetracycline labeling method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101735001A CN102265798B (en) | 2011-06-24 | 2011-06-24 | Sepia cuttlebone tetracycline labeling method |
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| Publication Number | Publication Date |
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| CN102265798A true CN102265798A (en) | 2011-12-07 |
| CN102265798B CN102265798B (en) | 2013-12-18 |
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| CN2011101735001A Active CN102265798B (en) | 2011-06-24 | 2011-06-24 | Sepia cuttlebone tetracycline labeling method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372272A (en) * | 2017-07-21 | 2017-11-24 | 浙江海洋大学 | A kind of cuttlefish long-acting physical device for marking based on endoskeleton cuttlebone |
| CN114403064A (en) * | 2022-03-09 | 2022-04-29 | 中国水产科学研究院黄海水产研究所 | Shell OTC marking method suitable for shellfish living bodies |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107904314A (en) * | 2017-11-08 | 2018-04-13 | 宁波大学 | A kind of molecular labeling and application available for Sepiella maindroni and golden cuttlefish species identification |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1961657A (en) * | 2006-11-26 | 2007-05-16 | 中国海洋大学 | A method for marking Sepiaesculenta |
-
2011
- 2011-06-24 CN CN2011101735001A patent/CN102265798B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1961657A (en) * | 2006-11-26 | 2007-05-16 | 中国海洋大学 | A method for marking Sepiaesculenta |
Non-Patent Citations (8)
| Title |
|---|
| M.D.DURHOLTZ等: "Laboratory validation of periodicity of incrementation in statoliths of the South African chokka squid Loligo vulgaris reynaudii(d"Orbigny,1845):a reevaluation", 《JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY》 * |
| R. VILLANUEVA: "Effect of temperature on statolith growth of the European squid Loligo vulgaris during early life", 《MARINE BIOLOGY》 * |
| 付自东等: "用荧光物质浸泡标记胭脂鱼仔、稚鱼耳石", 《动物学杂志》 * |
| 卢志晓等: "高效液相色谱法快速测定水产品中四环素类药物残留", 《现代仪器》 * |
| 孙松等: "海胆和海参的四环素标记", 《海洋科学》 * |
| 董智勇等: "曼氏无针乌贼荧光标记技术初步研究", 《浙江海洋学院学报（自然科学版）》 * |
| 郝振林等: "金乌贼荧光标志方法的研究", 《水产学报》 * |
| 陈新军等: "头足类年龄与生长特性的研究方法进展", 《大连水产学院学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372272A (en) * | 2017-07-21 | 2017-11-24 | 浙江海洋大学 | A kind of cuttlefish long-acting physical device for marking based on endoskeleton cuttlebone |
| CN107372272B (en) * | 2017-07-21 | 2020-10-13 | 浙江海洋大学 | Long-acting physical sign device of cuttlefish based on endoskeleton cuttlebone |
| CN114403064A (en) * | 2022-03-09 | 2022-04-29 | 中国水产科学研究院黄海水产研究所 | Shell OTC marking method suitable for shellfish living bodies |
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| Publication number | Publication date |
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| CN102265798B (en) | 2013-12-18 |
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Address after: 316000 No. 18 Sea Road, Dinghai, Dinghai, Zhejiang, Zhoushan, China Patentee after: Zhejiang Ocean University Address before: 316000 No. 18 Sea Road, Dinghai, Dinghai, Zhejiang, Zhoushan, China Patentee before: ZHEJIANG OCEAN University |
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Application publication date: 20111207 Assignee: Lvcheng Technology Services (Zhoushan) Co.,Ltd. Assignor: Zhejiang Ocean University Contract record no.: X2023980045987 Denomination of invention: Tetracycline labeling method for cuttlefish cuttlebone Granted publication date: 20131218 License type: Common License Record date: 20231106 |