CN102257961B - Tissue culture method for strawberry seedling - Google Patents

Tissue culture method for strawberry seedling Download PDF

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Publication number
CN102257961B
CN102257961B CN 201110139539 CN201110139539A CN102257961B CN 102257961 B CN102257961 B CN 102257961B CN 201110139539 CN201110139539 CN 201110139539 CN 201110139539 A CN201110139539 A CN 201110139539A CN 102257961 B CN102257961 B CN 102257961B
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culture
strawberry
days
strawberry seedling
seedling
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CN102257961A (en
Inventor
谢春光
许长江
赵士力
仇从宇
李廷月
孙克仕
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DONGHAI COUNTY HUANGCHUAN CHUNFENG STRAWBERRY PRODUCTION COOPERATIVE
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DONGHAI COUNTY HUANGCHUAN CHUNFENG STRAWBERRY PRODUCTION COOPERATIVE
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Abstract

The invention discloses a tissue culture method for a strawberry seedling. The method is characterized by comprising the following steps of: flushing a creeping stem grown on a spring female strawberry seedling, disinfecting, cleaning and performing primary culture at the temperature between 23-25 DEG C in dark environment for 5-8 days, and culturing according to lighting of 12-14 hours per day; and performing multiplication, rooting culture and domestication culture, wherein the relative air humidity is kept at 90 percent, the temperature is 15-20 DEG C and the shading rate is 75 percent during domestication culture. The tissue culture method for the strawberry seedling has high operability and is more reasonable; and according to the method the industrialization, professionalization and scale of the tissue culture of the strawberry seedling can be realized, the culture survival rate of the strawberry seedling can be increased, the yield and the quality of strawberry can be increased, and the economic efficiency of strawberry planting can be increased.

Description

A kind of method for tissue culture of strawberry seedling
Technical field
The present invention relates to the method for tissue culture of the method for tissue culture of a Plants, particularly a kind of strawberry seedling.
Background technology
In recent years, occur yield reducation, fruit deformity in the strawberry production, go on the market slower degradation trend under the economic benefit.Its main cause is to lack strawberry high quality seedling production technology on the one hand, and the way that adopts tradition to grow seedlings in the production causes the virus accumulation serious, makes original kind of sexual involution of strawberry, and quality and yield significantly descends; Be that yellow cultivated strawberry mode is more backward on the other hand, lack the ahead of time efficiency cultivation technology for high of listing of forcing culture, thereby the person's that makes the strawberry cultivating planting benefit descends.
Summary of the invention
Technical problem to be solved by this invention is for the deficiencies in the prior art, provide a kind of method more reasonable, can grasp strong with property, can realize the efficient method for tissue culture that carries the strawberry seedling of training.
Technical problem to be solved by this invention is to realize by following technical scheme.The present invention is a kind of method for tissue culture of strawberry seedling, is characterized in, its step is as follows:
(1) sterilization: choose the stolon that the female Strawberry Seedlings in spring occurs and make explant, after the water flushing, then with 70% alcohol-pickled sterilization 11-13 after second, sterile water wash is then used 0.1%Hgcl 2Sterilize after 13-18 minute, use again sterile water wash;
(2) first culture: under carrying stereomicroscope, cut in the female Strawberry Seedlings stem apex access medium after the disinfecting of 0.3mm and carried out just culture 55-60 days; Just the temperature of culture is 23-25 ℃, places in the dark first during cultivation and cultivates after 5-8 days, cultivates by 12-14 hour/day illumination again, with 75% alcohol environment is carried out disinfection once in per 2 days during cultivation, and ultraviolet ray carries out disinfection 1 time to environment weekly;
(3) propagation, culture of rootage: the Strawberry Seedlings of first culture is bred cultivation, and the cycle that propagation is cultivated is 30 days, and propagation is cultivated 2 all after dates, is divided into individual plant, moves into and carries out hairy root culture in the medium 20 days;
(4) domestication is cultivated: the Strawberry Seedlings of culture of rootage is moved into the peat composed of rotten mosses and perlite mix that (its mixed proportion can be allocated on demand, the general peat composed of rotten mosses accounts for about the 10-70% of mixture weight best) domestication matrix in tame to cultivate and namely got strawberry seedling in 7 days, keeping the relative moisture of air during cultivation is 90%, temperature is 15-20 ℃, and shading rate is 75%;
Consisting of of described medium:
NH 4NO 3 82.5g ; KNO 3 95g ; KH 2PO 4 8.5g ;
H 3BO 3 310mg ; MnSO 4·4H 2O 18.95mg ;
ZnSO 4·7H 2O 53mg ; KI 41.5mg ; Na 2MoO 4·2H 2O 12.5mg;
CuSO 4·5H 2O 1.25mg ; CoCl 2·6H 2O 1.25mg;
CaCl 2·2H 2O 22g ; MgSO 4·7H 2O 18. 5g ;
FeSO 4·7H 2O 1.865g ; Na 2-EDTA 1.39g ;
Inositol 10g; Nicotinic acid 50mg; Pyridoxine 50mg;
Thiamines acid 10mg; Glycine 200mg;
After above-mentioned each component mixed, add pure water to 1L, to 5.8-5.9, be heated to boiling after adding again sucrose 30g, agar-agar 8g with the 1mol/LNaOH adjust pH; Be sub-packed in and be heated to 121 ℃ and keep and carried out disinfection in 20 minutes behind the blake bottle, cooling namely gets medium naturally.
Compared with prior art; the inventive method is a kind of method for tissue culture of workable, the more rational strawberry seedling of method; it can realize batch production, specialization and the scale of the tissue cultivation of strawberry seedling; and the inventive method can improve year training survival rate of strawberry seedling; improve the yield and quality of strawberry, increase the economic benefit of strawberry cultivating.
Embodiment
Below further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further, and do not consist of its Copyright law.
Embodiment 1.A kind of method for tissue culture of strawberry seedling, its step is as follows:
(1) sterilization: the stolon of choosing female Strawberry Seedlings generation in spring is made explant, after the water flushing, then uses 70% alcohol-pickled sterilization after 11 seconds, and sterile water wash is then used 0.1%Hgcl 2Sterilize after 13-18 minute, use again sterile water wash;
(2) first culture: under carrying stereomicroscope, cut and carried out just culture in the female Strawberry Seedlings stem apex access medium after the disinfecting of 0.3mm 55 days; Just the temperature of culture is 23 ℃, places in the dark first during cultivation and cultivates after 5 days, cultivates by 12 hours/day illumination again, with 75% alcohol environment is carried out disinfection once in per 2 days during cultivation, and ultraviolet ray carries out disinfection 1 time to environment weekly;
(3) propagation, culture of rootage: the Strawberry Seedlings of first culture is bred cultivation, and the cycle that propagation is cultivated is 30 days, and propagation is cultivated 2 all after dates, is divided into individual plant, moves into and carries out hairy root culture in the medium 20 days;
(4) domestication is cultivated: the Strawberry Seedlings of culture of rootage is moved into tame cultivation in the domestication matrix that the peat composed of rotten mosses and perlite mix (its mixed proportion is 4:6) and namely got strawberry seedling in 7 days, keeping the relative moisture of air during cultivation is 90%, temperature is 15 ℃, and shading rate is 75%;
Consisting of of described medium:
NH 4NO 3 82.5g ; KNO 3 95g ; KH 2PO 4 8.5g ;
H 3BO 3 310mg ; MnSO 4·4H 2O 18.95mg ;
ZnSO 4·7H 2O 53mg ; KI 41.5mg ; Na 2MoO 4·2H 2O 12.5mg;
CuSO 4·5H 2O 1.25mg ; CoCl 2·6H 2O 1.25mg;
CaCl 2·2H 2O 22g ; MgSO 4·7H 2O 18. 5g ;
FeSO 4·7H 2O 1.865g ; Na 2-EDTA 1.39g ;
Inositol 10g; Nicotinic acid 50mg; Pyridoxine 50mg;
Thiamines acid 10mg; Glycine 200mg;
After above-mentioned each component mixed, add pure water to 1L, behind 1mol/LNaOH adjust pH to 5.8, be heated to boiling after adding again sucrose 30g, agar-agar 8g; Be sub-packed in and be heated to 121 ℃ and keep and carried out disinfection in 20 minutes behind the blake bottle, cooling namely gets medium naturally.
Embodiment 2.A kind of method for tissue culture of strawberry seedling, its step is as follows:
(1) sterilization: the stolon of choosing female Strawberry Seedlings generation in spring is made explant, after the water flushing, then uses 70% alcohol-pickled sterilization after 13 seconds, and sterile water wash is then used 0.1%Hgcl 2Sterilize after 18 minutes, use again sterile water wash;
(2) first culture: under carrying stereomicroscope, cut and carried out just culture in the female Strawberry Seedlings stem apex access medium after the disinfecting of 0.3mm 60 days; Just the temperature of culture is 25 ℃, places in the dark first during cultivation and cultivates after 8 days, cultivates by 12-14 hour/day illumination again, with 75% alcohol environment is carried out disinfection once in per 2 days during cultivation, and ultraviolet ray carries out disinfection 1 time to environment weekly;
(3) propagation, culture of rootage: the Strawberry Seedlings of first culture is bred cultivation, and the cycle that propagation is cultivated is 30 days, and propagation is cultivated 2 all after dates, is divided into individual plant, moves into and carries out hairy root culture in the medium 20 days;
(4) domestication is cultivated: the Strawberry Seedlings of culture of rootage is moved into tame cultivation in the domestication matrix that the peat composed of rotten mosses and perlite mix (its mixed proportion is 5:5) and namely got strawberry seedling in 7 days, keeping the relative moisture of air during cultivation is 90%, temperature is 20 ℃, and shading rate is 75%;
Consisting of of described medium:
NH 4NO 3 82.5g ; KNO 3 95g ; KH 2PO 4 8.5g ;
H 3BO 3 310mg ; MnSO 4·4H 2O 18.95mg ;
ZnSO 4·7H 2O 53mg ; KI 41.5mg ; Na 2MoO 4·2H 2O 12.5mg;
CuSO 4·5H 2O 1.25mg ; CoCl 2·6H 2O 1.25mg;
CaCl 2·2H 2O 22g ; MgSO 4·7H 2O 18. 5g ;
FeSO 4·7H 2O 1.865g ; Na 2-EDTA 1.39g ;
Inositol 10g; Nicotinic acid 50mg; Pyridoxine 50mg;
Thiamines acid 10mg; Glycine 200mg;
After above-mentioned each component mixed, add pure water to 1L, behind 1mol/LNaOH adjust pH to 5.9, be heated to boiling after adding again sucrose 30g, agar-agar 8g; Be sub-packed in and be heated to 121 ℃ and keep and carried out disinfection in 20 minutes behind the blake bottle, cooling namely gets medium naturally.

Claims (1)

1. the method for tissue culture of a strawberry seedling is characterized in that, its step is as follows:
(1) sterilization: choose the stolon that the female Strawberry Seedlings in spring occurs and make explant, after the water flushing, then with 70% alcohol-pickled sterilization 11-13 after second, sterile water wash is then used 0.1%HgCl 2Sterilize after 13-18 minute, use again sterile water wash;
(2) first culture: under carrying stereomicroscope, cut in the female Strawberry Seedlings stem apex access medium after the disinfecting of 0.3mm and carried out just culture 55-60 days; Just the temperature of culture is 23-25 ℃, places in the dark first during cultivation and cultivates after 5-8 days, cultivates by 12-14 hour/day illumination again, with 75% alcohol environment is carried out disinfection once in per 2 days during cultivation, and ultraviolet ray carries out disinfection 1 time to environment weekly;
(3) propagation, culture of rootage: the Strawberry Seedlings of first culture is bred cultivation, and the cycle that propagation is cultivated is 30 days, and propagation is cultivated 2 all after dates, is divided into individual plant, moves into and carries out hairy root culture in the medium 20 days;
(4) domestication is cultivated: the Strawberry Seedlings of culture of rootage is moved into to tame to cultivate in the domestication matrix that the peat composed of rotten mosses and perlite mix namely got strawberry seedling in 7 days, the relative moisture of maintenance air is 90% during cultivation, and temperature is 15-20 ℃, and shading rate is 75%;
Consisting of of described medium:
NH 4NO 3 82.5g;KNO 3 95g;KH 2PO 4 8.5g;
H 3BO 3 310mg;MnSO 4·4H 2O 18.95mg;
ZnSO 4·7H 2O 53mg;KI 41.5mg;Na 2MoO 4·2H 2O 12.5mg;
CuSO 4·5H 2O 1.25mg;CoCl 2·6H 2O 1.25mg;
CaCl 2·2H 2O 22g;MgSO 4·7H 2O 18. 5g;
FeSO 4·7H 2O 1.865g;Na 2-EDTA 1.39g;
Inositol 10g; Nicotinic acid 50mg; Pyridoxine 50mg;
Thiamines acid 10mg; Glycine 200mg;
After above-mentioned each component mixed, add pure water to 1L, to 5.8-5.9, be heated to boiling after adding again sucrose 30g, agar 8g with the 1mol/LNaOH adjust pH; Be sub-packed in and be heated to 121 ℃ and keep and carried out disinfection in 20 minutes behind the blake bottle, cooling namely gets medium naturally.
CN 201110139539 2011-05-27 2011-05-27 Tissue culture method for strawberry seedling Expired - Fee Related CN102257961B (en)

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Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102668987B (en) * 2012-05-31 2013-07-17 句容市白兔镇云兔草莓专业合作社 Multiplying culture media for strawberry
CN103283594B (en) * 2013-05-13 2014-07-16 北京林业大学 Micro-propagation liquid culture method for strawberries
CN103283595B (en) * 2013-05-17 2015-04-22 高红胜 Primary culture method of stem tip tissue of strawberry
CN104782618A (en) * 2015-04-14 2015-07-22 阜阳市殿兴农业科技有限公司 Strawberry seedling tissue preservation method
CN104770301A (en) * 2015-04-22 2015-07-15 吴迪 Tissue culture method for strawberry seedlings
CN104904598A (en) * 2015-06-01 2015-09-16 张亚南 Method for cultivating strawberry seedlings
CN106576735A (en) * 2016-11-16 2017-04-26 唐滨 Strontium-rich dry lotus root planting method using tissue culture seedlings
CN110178690B (en) * 2019-06-12 2021-11-05 贵州金盘水农业科技有限公司 Application of carbon nano sol in strawberry cultivation, strawberry seedling cultivation medium, matrix and strawberry seedling cultivation method
CN112425508B (en) * 2020-12-08 2022-10-25 黑龙江省农业科学院园艺分院 Culture medium for inducing differentiation, proliferation and rooting of strawberry stem tips

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Publication number Priority date Publication date Assignee Title
CN1454464A (en) * 2002-11-26 2003-11-12 中国科学院黑龙江农业现代化研究所 Strawberry anther regenerative strain-induced culture medium
CN1423927A (en) * 2003-01-13 2003-06-18 贾景明 Strawberry seedling production by tissue culture
CN100362908C (en) * 2005-12-26 2008-01-23 浙江大学 Strawberry virus elimination method

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