CN1423927A - Strawberry seedling production by tissue culture - Google Patents
Strawberry seedling production by tissue culture Download PDFInfo
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- CN1423927A CN1423927A CN 03110849 CN03110849A CN1423927A CN 1423927 A CN1423927 A CN 1423927A CN 03110849 CN03110849 CN 03110849 CN 03110849 A CN03110849 A CN 03110849A CN 1423927 A CN1423927 A CN 1423927A
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- strawberry
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Abstract
A tissue culture method for preparing strawberry includes culturing the leaf of strawberry in the culture medium MB to regenerate the seedlings of strawberry, which is a clonic straw berry. Its advantaegs are high regeneration rate (97% or more) and rooting rate up to 100%, low cost and no environmental pollution.
Description
Technical field: the present invention relates to a kind of method of producing the strawberry seedling by Plant Tissue Breeding.Specifically be to be means with the plant tissue culture technique,, and obtain the method for regeneration strawberry seedlings in a large number by cultivation strawberry blade on the certain culture base.The invention belongs to agricultural biological technical field.
Background technology: strawberry (Fragaria * ananassa Duch.) belongs to rose family Fragaria herbaceos perennial, its berry is the first-class fruit that nutritive value is extremely abundant, economic worth is very high, have the title of " fruit queen ", not only contain vitamin than high ten times of other fruit, and contained ellagic acid has anticancer effect in the fruit, and therefore in very great demand in American-European market, price is five times of apple, two times of grape, still supply falls short of demand.Japan, Southeast Asia and American-European countries all require from up to ten thousand tons of quick-frozen strawberries of the annual import of China, are the unique export Fruit of China.This is because in Japan, Southeast Asia and American-European countries, value of labour power is very high, and the cost that needs 1 kilogram of strawberry of the every production of Japan just reaches 20 yuans.And comprise that from 1 kilogram of strawberry of China's import freight charges only are 10 yuan.Therefore Japan, Southeast Asia and American-European countries all require to enlarge the strawberry import one after another.Produce so greatly develop China strawberry, satisfy growing demand for exports and will be the important behave of rich people, production poverty alleviation of benefiting the nation.For a long time, though by conventional breeding aspects such as strawberry output, fruit size and quality are improved to some extent, planting strawberry is the assorted and body of height, has polyploidy in the heredity again, and therefore this improvement is slowly, also is limited.Simultaneously, the incompatibility of distant hybridization and limited genetic resources have also hindered the process of conventional hybridization breeding, especially cultivating aspect the strawberry cultivars such as antiviral, antimycotic, antiweed, degeneration-resistant border, storage endurance, all be difficult to realization because of can not find suitable parent.Produce the problem that faces for a long time and will solve these strawberries, the person opens up new technology path just to need the breeding work, creates new breeding approach.Therefore, in order to enlarge strawberry market occupancy volume, pursue low-cost, high-quality and high benefit, develop the new mode of breeding, become the hot issue of current strawberry market development. utilization cell engineering means, breed strawberry fast by method for plant tissue culture, hybrid vigor fixing, keep varietY specificity, facts have proved it is feasible. utilize strawberry to be organized in and carry out in-vitro propagate on the proper culture medium, can accelerate variety breeding speed, enlarge provenance production, the proterties that keeps improved seeds, with the less space that gets, less individuality can obtain heredity with the less time and go up a large amount of regeneration nontoxic seedling identical with parental plant. and this modes of reproduction is that an effective way has been opened up in the industrialized development of strawberry, and its social benefit and economic benefit are huge.
We are through years, analyzing, studying on the basis of domestic and international strawberry achievement in research, systematically study plant tissue cell's culture technique and be applied to theory, technology and the feasibility that strawberry produces, comprise from processes such as material selection, explant induction, high frequency regeneration and field planting, laboratory and pilot scale research have been finished, make next step industrialization have more practical significance, and provide for this reason may with guarantee.
Summary of the invention: the object of the present invention is to provide a kind of raising Plant Tissue Breeding strawberry seedling productivity ratio, make it reach industrialization level.This project is through research for many years, and according to the plant leaf blade regeneration principle, having initiated with the blade is explant, sets up high frequency stable regeneration system.For realizing purpose of the present invention, adopt following technical proposals:
Selection has 30---the strawberry young leaflet tablet of 45 days seedling ages, to cut off at its blade middle part, and adaxial and its surface takes place as for inducing embryoid body on the regeneration culture medium downwards.On the basis of the above, the cultivation of leaf regeneration indefinite bud was an one-period in 15 days.
Cultivation temperature 25 ℃ ± 1, intensity of illumination 2500lux, light application time 14h/d.
Induce that to form with regeneration culture medium be the MB minimal medium, i.e. the macroelement B of MS medium
5The trace element combination of medium, additional simultaneously 6-BA 0---4.0mg/l, IBA0---1.5mg/l.
The strawberry seedling of regeneration was cultivated after 40 days, can directly forward on the MB medium and take root.
The 6-BA that is added, IBA consumption are: 6-BA 0-4.0mg/l, IBA 0---1.5mg/l.
Need to add sucrose 1.5-3.0% in the above-mentioned medium, and transfer P
HValue 4.5-6.5.
Above-mentioned condition of culture is 14h/d illumination, 2500Lx intensity of illumination, temperature 25 ℃ ± 1.
Best Regeneration in Vitro condition of culture of the present invention is:
MB medium: additional 6-BA 3.0mg/l+IBA 0.5mg/l+ sucrose 2.5%.
The invention provides a kind of method of strawberry rapid propagation in vitro, is to cultivate the strawberry blade on the MB medium, and regeneration strawberry seedling obtains the strawberry clone.The strawberry regeneration rate reaches more than 97.0%, and rooting rate reaches 100%.This method can not only obviously improve the quality and quantity of strawberry regrowth, and it is low, free from environmental pollution to have a production cost, reaches the characteristics of industrialization level, and its development prospect is very good.
Embodiment:
This technology is with the different genotype kind: the Hani, the precious friendship, beautiful treasured, Feng Xiang, U.S. fragrant Sha and early Feng Jinhang cultivate.Explant all selects to have 30---the strawberry young leaflet tablet of 45 days seedling ages.Down its blade middle part is cut off aseptic, adaxial and its surface is downwards as for regeneration culture medium MB: on the additional 6-BA 3.0mg/l mg/l+IBA0.5mg/l+ sucrose 2.5%, pH value is 5.5 ± 1.Cultivate 15 days rear blade regenerated adventitious buds.On regeneration culture medium MB, carry out successive transfer culture, after regrowth is cultivated 40 days approximately, can directly forward on the MB medium and take root.Whole incubation condition is: 25 ± 1 ℃ of cultivation temperature, intensity of illumination 2500lux, light application time 14h/d.
The different cultivars blade the regeneration on the MB culture medium as a result the single blade average regeneration frequency of average regeneration bud number (%) rooting rate (%) of kind Hani 15.4 99.6 100 precious hand over 13.0 97.1 100 beautiful precious 12.8 97.0 100 Feng Xiang, 14.7 98.8 100 U.S. fragrant Sha 13.5 97.5 100 early rich 13.3 97.3 100 on the MB culture medium the average regeneration bud number of single blade of 6 strawberry cultivars more than 13%; Average regeneration rate is more than 97%, and the regrowth rooting rate all reaches 100%.
Claims (7)
1, the method for strawberry seedling is produced in a kind of Plant Tissue Breeding, it is characterized in that this method is included in to carry out blade on the MB medium and cultivate, and carries out successive transfer culture on the MB medium, obtains the strawberry clone.
2, the method for strawberry seedling is produced in Plant Tissue Breeding according to claim 1, it is characterized in that the strawberry blade of choosing is to have 30---the young leaflet tablet of 45 days seedling ages, to cut off at its blade middle part, and adaxial and its surface is downwards as on the regeneration culture medium.
3, the method for strawberry seedling is produced in Plant Tissue Breeding according to claim 1, it is characterized in that it is one-period that the leaf regeneration indefinite bud is cultivated 15 days.
4, the method for strawberry seedling is produced in Plant Tissue Breeding according to claim 1, it is characterized in that 25 ± 1 ℃ of cultivation temperature, intensity of illumination 2500lux, light application time 14 hours/day.
5, the method for strawberry seedling is produced in Plant Tissue Breeding according to claim 1, it is characterized in that inducing that to form with regeneration culture medium be the MB minimal medium, it is the trace element combination of the macroelement B5 medium of MS medium, additional simultaneously 6-BA O---4.Omg/l, IBA 0---1.5mg/l.
6, the method for strawberry seedling is produced in Plant Tissue Breeding according to claim 1, it is characterized in that pH value is 5.5 ± 1.
7, the method for strawberry seedling is produced in Plant Tissue Breeding according to claim 1, and the strawberry seedling cultivation that it is characterized in that regenerating can directly forward on the MB medium and take root after 40 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03110849 CN1423927A (en) | 2003-01-13 | 2003-01-13 | Strawberry seedling production by tissue culture |
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| CN 03110849 CN1423927A (en) | 2003-01-13 | 2003-01-13 | Strawberry seedling production by tissue culture |
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| CN1423927A true CN1423927A (en) | 2003-06-18 |
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| CN 03110849 Pending CN1423927A (en) | 2003-01-13 | 2003-01-13 | Strawberry seedling production by tissue culture |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101836591A (en) * | 2010-06-03 | 2010-09-22 | 中国农业大学 | Method for culturing axillary bud tissue of strawberry |
| CN101606489B (en) * | 2009-07-25 | 2011-09-07 | 通化师范学院 | Cultivating method for inducing variation plant variety by using strawberry petal in remote mountains |
| CN102210253A (en) * | 2010-04-12 | 2011-10-12 | 江苏省农业科学院 | Method for improving seedling emergence of strawberry seeds |
| CN102257961A (en) * | 2011-05-27 | 2011-11-30 | 东海县黄川春风草莓生产合作社 | Tissue culture method for strawberry seedling |
| CN102499093A (en) * | 2011-11-23 | 2012-06-20 | 天津中植科生物技术有限公司 | Novel high-efficient quick virus elimination technique for strawberries |
| CN102893861A (en) * | 2012-02-21 | 2013-01-30 | 张子学 | Technology for regenerating and recycling plant tissue medium polluted by undesired bacteria |
| CN104396503A (en) * | 2014-11-10 | 2015-03-11 | 福建省农业科学院生物技术研究所 | Summer acclimation transition method for strawberry tissue culture seedlings |
| CN107333659A (en) * | 2017-09-15 | 2017-11-10 | 江苏省农业科学院 | A kind of day neutrality Strawberry Plantlets fast breeding culture medium and tissue culture and rapid propagation method |
| CN108094198A (en) * | 2017-12-07 | 2018-06-01 | 陕西师范大学 | A kind of Strawberry Leaves in-vitro regeneration method |
-
2003
- 2003-01-13 CN CN 03110849 patent/CN1423927A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101606489B (en) * | 2009-07-25 | 2011-09-07 | 通化师范学院 | Cultivating method for inducing variation plant variety by using strawberry petal in remote mountains |
| CN102210253A (en) * | 2010-04-12 | 2011-10-12 | 江苏省农业科学院 | Method for improving seedling emergence of strawberry seeds |
| CN101836591A (en) * | 2010-06-03 | 2010-09-22 | 中国农业大学 | Method for culturing axillary bud tissue of strawberry |
| CN102257961A (en) * | 2011-05-27 | 2011-11-30 | 东海县黄川春风草莓生产合作社 | Tissue culture method for strawberry seedling |
| CN102499093A (en) * | 2011-11-23 | 2012-06-20 | 天津中植科生物技术有限公司 | Novel high-efficient quick virus elimination technique for strawberries |
| CN102893861A (en) * | 2012-02-21 | 2013-01-30 | 张子学 | Technology for regenerating and recycling plant tissue medium polluted by undesired bacteria |
| CN104396503A (en) * | 2014-11-10 | 2015-03-11 | 福建省农业科学院生物技术研究所 | Summer acclimation transition method for strawberry tissue culture seedlings |
| CN104396503B (en) * | 2014-11-10 | 2017-02-15 | 福建省农业科学院生物技术研究所 | Summer acclimation transition method for strawberry tissue culture seedlings |
| CN107333659A (en) * | 2017-09-15 | 2017-11-10 | 江苏省农业科学院 | A kind of day neutrality Strawberry Plantlets fast breeding culture medium and tissue culture and rapid propagation method |
| CN107333659B (en) * | 2017-09-15 | 2019-01-22 | 江苏省农业科学院 | A kind of day neutral Strawberry Plantlets fast breeding culture medium and tissue culture and rapid propagation method |
| CN108094198A (en) * | 2017-12-07 | 2018-06-01 | 陕西师范大学 | A kind of Strawberry Leaves in-vitro regeneration method |
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