CN102230007B - 一种分子信标荧光定量pcr检测邻苯二甲酸酯的方法 - Google Patents
一种分子信标荧光定量pcr检测邻苯二甲酸酯的方法 Download PDFInfo
- Publication number
- CN102230007B CN102230007B CN 201110156577 CN201110156577A CN102230007B CN 102230007 B CN102230007 B CN 102230007B CN 201110156577 CN201110156577 CN 201110156577 CN 201110156577 A CN201110156577 A CN 201110156577A CN 102230007 B CN102230007 B CN 102230007B
- Authority
- CN
- China
- Prior art keywords
- molecular beacon
- dna
- phthalic ester
- paes
- quantitative pcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000003753 real-time PCR Methods 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 20
- 150000003021 phthalic acid derivatives Chemical class 0.000 title 1
- 238000001514 detection method Methods 0.000 claims abstract description 23
- 101710163270 Nuclease Proteins 0.000 claims abstract description 9
- 210000004185 liver Anatomy 0.000 claims abstract description 9
- 210000000172 cytosol Anatomy 0.000 claims abstract description 7
- 241000252233 Cyprinus carpio Species 0.000 claims abstract description 5
- 238000004458 analytical method Methods 0.000 claims abstract description 3
- 238000012417 linear regression Methods 0.000 claims abstract description 3
- 150000002148 esters Chemical class 0.000 claims description 41
- 238000011534 incubation Methods 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 18
- 239000000523 sample Substances 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000013016 damping Methods 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- 238000011144 upstream manufacturing Methods 0.000 claims description 8
- 238000012408 PCR amplification Methods 0.000 claims description 7
- 230000008859 change Effects 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 239000012089 stop solution Substances 0.000 claims description 5
- 239000002585 base Substances 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 3
- 239000007983 Tris buffer Substances 0.000 claims description 3
- 238000000246 agarose gel electrophoresis Methods 0.000 claims description 3
- 239000003513 alkali Substances 0.000 claims description 3
- 238000000137 annealing Methods 0.000 claims description 3
- 239000008280 blood Substances 0.000 claims description 3
- 210000004369 blood Anatomy 0.000 claims description 3
- 239000000872 buffer Substances 0.000 claims description 3
- 230000009849 deactivation Effects 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 3
- 239000013612 plasmid Substances 0.000 claims description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- 229920002684 Sepharose Polymers 0.000 claims description 2
- 108010006785 Taq Polymerase Proteins 0.000 claims description 2
- 238000000746 purification Methods 0.000 claims description 2
- 239000011535 reaction buffer Substances 0.000 claims description 2
- 108010052305 exodeoxyribonuclease III Proteins 0.000 abstract description 7
- 150000001875 compounds Chemical class 0.000 abstract description 5
- 230000003321 amplification Effects 0.000 abstract description 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 238000001976 enzyme digestion Methods 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 26
- 238000002360 preparation method Methods 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 230000007613 environmental effect Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 102000005962 receptors Human genes 0.000 description 4
- 108020003175 receptors Proteins 0.000 description 4
- 102000015694 estrogen receptors Human genes 0.000 description 3
- 108010038795 estrogen receptors Proteins 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- BLFLLBZGZJTVJG-UHFFFAOYSA-N benzocaine Chemical compound CCOC(=O)C1=CC=C(N)C=C1 BLFLLBZGZJTVJG-UHFFFAOYSA-N 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 229940011871 estrogen Drugs 0.000 description 2
- 239000000262 estrogen Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 108091027981 Response element Proteins 0.000 description 1
- CWHJIJJSDGEHNS-MYLFLSLOSA-N Senegenin Chemical compound C1[C@H](O)[C@H](O)[C@@](C)(C(O)=O)[C@@H]2CC[C@@]3(C)C(CC[C@]4(CCC(C[C@H]44)(C)C)C(O)=O)=C4[C@@H](CCl)C[C@@H]3[C@]21C CWHJIJJSDGEHNS-MYLFLSLOSA-N 0.000 description 1
- 239000004902 Softening Agent Substances 0.000 description 1
- 230000007059 acute toxicity Effects 0.000 description 1
- 231100000403 acute toxicity Toxicity 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960005274 benzocaine Drugs 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000010931 ester hydrolysis Methods 0.000 description 1
- 230000001076 estrogenic effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 238000006303 photolysis reaction Methods 0.000 description 1
- 238000003822 preparative gas chromatography Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000009871 tenuigenin Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201110156577 CN102230007B (zh) | 2011-06-10 | 2011-06-10 | 一种分子信标荧光定量pcr检测邻苯二甲酸酯的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201110156577 CN102230007B (zh) | 2011-06-10 | 2011-06-10 | 一种分子信标荧光定量pcr检测邻苯二甲酸酯的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102230007A CN102230007A (zh) | 2011-11-02 |
CN102230007B true CN102230007B (zh) | 2013-10-09 |
Family
ID=44842612
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 201110156577 Expired - Fee Related CN102230007B (zh) | 2011-06-10 | 2011-06-10 | 一种分子信标荧光定量pcr检测邻苯二甲酸酯的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102230007B (zh) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102608055B (zh) * | 2012-03-08 | 2014-04-02 | 中国科学院合肥物质科学研究院 | 食品中苯二甲酸酯类塑化剂的快速荧光检测方法 |
CN110530835B (zh) * | 2019-09-27 | 2022-03-04 | 山西省农业科学院农产品加工研究所 | 油类物质中邻苯二甲酸酯类增塑剂的荧光法检测方法 |
CN110849859B (zh) * | 2019-12-17 | 2021-03-23 | 山东农业大学 | 基于双发射碳量子点标记核酸适配体的荧光比值法检测邻苯二甲酸二丁酯的方法 |
CN113933272B (zh) * | 2021-09-28 | 2022-08-09 | 云南省农业科学院农产品加工研究所 | 食品中邻苯二甲酸酯类塑化剂的荧光探针检测方法 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101691606B (zh) * | 2009-08-20 | 2012-11-07 | 东华大学 | 一种外切酶保护荧光定量pcr检测壬基酚的方法 |
-
2011
- 2011-06-10 CN CN 201110156577 patent/CN102230007B/zh not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CN102230007A (zh) | 2011-11-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102230007B (zh) | 一种分子信标荧光定量pcr检测邻苯二甲酸酯的方法 | |
CN111378679A (zh) | 一种基因表达组件及其构建的克隆载体和应用 | |
KR20130068530A (ko) | 세포배양에서 엑소좀의 정량 방법 및 이를 이용한 엑소좀의 수득율을 증가시키는 방법 | |
CN108623696B (zh) | 一种利用纤维素固定化CBD-EndoS融合酶的方法 | |
CN107058277B (zh) | 一种大豆蛋白的应用及其制备方法和表达载体、引物 | |
KR101629353B1 (ko) | 구제역 a형 표준 백신주의 방어항원이 발현되는 재조합 구제역 바이러스 및 그의 제조방법 | |
CN108642074A (zh) | 一种含有乙醇诱导启动子的质粒载体及其在提高谷氨酸棒杆菌重组蛋白表达量中的应用 | |
KR20130140934A (ko) | 세포주, 세포 배양액 또는 생체 시료의 마이크로베지클로부터 마이크로rna를 직접 추출하는 방법 | |
CN113913421A (zh) | 全血dna提取试剂及提取方法 | |
CN105671068B (zh) | 一种基于人源骨架蛋白Fn3制备脑钠肽前体抗原替代物的方法 | |
CN108676848B (zh) | 用于检测融合基因的混合基因、标准质粒、试剂盒及其制备方法 | |
CN107151670B (zh) | 大豆蛋白及其编码基因的应用及引物、表达载体和制备方法 | |
CN106893733A (zh) | 一种基于大肠杆菌表达系统的重组pBpp蛋白制备方法 | |
CN110042067A (zh) | 一种提高重组酿酒酵母菌株木糖利用能力的方法及其突变株 | |
CN108866031B (zh) | 大豆蛋白的应用 | |
CN111139209B (zh) | 表达HER2单链抗体的重组大肠杆菌Nissle 1917及其功能验证方法 | |
CN108085371B (zh) | 判断pcr结果是否为假阳性的方法 | |
CN114438163B (zh) | 真菌筛选试剂、筛选方法、试剂盒及应用 | |
CN101038517A (zh) | 一种汉字形声编码输入法 | |
CN108070532A (zh) | 一种生产葡萄糖氧化酶的方法 | |
JPH10248585A (ja) | 天然物および他の化学物質をスクリーニングするためのインビトロ転写法 | |
CN112592844B (zh) | 一种重组毕赤酵母基因工程菌及其构建方法与在产木聚糖酶中的应用 | |
CN108949793B (zh) | 一种表征遗传毒性的重组菌及其构建方法与应用 | |
CN109136236A (zh) | 一种提高铅结合蛋白特异性的定向进化方法 | |
CN101481703A (zh) | 禽源启动子表达载体及其构建方法与应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CB03 | Change of inventor or designer information |
Inventor after: Zhang Yanliang Inventor before: Zhao Xiaoxiang Inventor before: Bai Zhou Inventor before: Li Hao Inventor before: Shao Panshan Inventor before: Xie Jia |
|
CB03 | Change of inventor or designer information | ||
TR01 | Transfer of patent right |
Effective date of registration: 20170605 Address after: Zhongxing Road, yangshe Bridge Village in Suzhou city Jiangsu province 215600 Zhangjiagang City No. 18 Patentee after: SUZHOU DATA TESTING SERVICE TECHNOLOGY CO.,LTD. Address before: 201620 Shanghai Songjiang new town, North Renmin Road, No. 2999, No. Patentee before: Donghua University |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20220527 Address after: No. 288, group 2, Yinhe village, Chahe Town, Rudong County, Nantong City, Jiangsu Province, 226000 Patentee after: Nantong Jiahuan Environmental Technology Co.,Ltd. Address before: 215600 No.18 Zhongxing Road, Xieqiao village, yangshe Town, Zhangjiagang City, Suzhou City, Jiangsu Province Patentee before: SUZHOU DATA TESTING SERVICE TECHNOLOGY CO.,LTD. |
|
TR01 | Transfer of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131009 |