CN102216456A - 基于甲基化富集母体样品中胎儿核酸的非侵入性产前诊断用方法和组合物 - Google Patents

基于甲基化富集母体样品中胎儿核酸的非侵入性产前诊断用方法和组合物 Download PDF

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CN102216456A
CN102216456A CN2009801458565A CN200980145856A CN102216456A CN 102216456 A CN102216456 A CN 102216456A CN 2009801458565 A CN2009801458565 A CN 2009801458565A CN 200980145856 A CN200980145856 A CN 200980145856A CN 102216456 A CN102216456 A CN 102216456A
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nucleic acid
fetal
dna
maternal
methylation
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M·埃里希
A·O·H·尼格伦
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Sequenom Inc
Sequenom Center for Molecular Medicine LLC
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CN2009801458565A 2008-09-16 2009-09-16 基于甲基化富集母体样品中胎儿核酸的非侵入性产前诊断用方法和组合物 Pending CN102216456A (zh)

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US19226408P 2008-09-16 2008-09-16
US61/192,264 2008-09-16
PCT/US2009/057215 WO2010033639A2 (en) 2008-09-16 2009-09-16 Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses

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EP (3) EP3103871B1 (enExample)
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AU (1) AU2009293232B2 (enExample)
CA (4) CA3209502A1 (enExample)
DK (1) DK2329021T3 (enExample)
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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105164509A (zh) * 2012-08-28 2015-12-16 阿科尼生物系统公司 用于纯化核酸的方法和试剂盒
CN107223159A (zh) * 2014-05-09 2017-09-29 科戴克斯生命股份公司 源自特定细胞类型的dna的检测及相关方法
US10093919B2 (en) 2007-10-31 2018-10-09 Akonni Biosystems, Inc. Method and kit for purifying nucleic acids
CN109689896A (zh) * 2015-11-10 2019-04-26 科戴克斯生命股份公司 使用在胎儿和怀孕雌性动物之间差异甲基化的dna区域检测胎儿染色体非整倍性
CN110734907A (zh) * 2019-09-17 2020-01-31 上海凌恩生物科技有限公司 一种叶绿体dna的富集方法
CN111755072A (zh) * 2020-08-04 2020-10-09 深圳吉因加医学检验实验室 一种同时检测甲基化水平、基因组变异和插入片段的方法及装置
CN112037860A (zh) * 2013-06-13 2020-12-04 阿瑞奥萨诊断公司 用于非入侵性性染色体非整倍性确定的统计分析
CN116246704A (zh) * 2023-05-10 2023-06-09 广州精科生物技术有限公司 用于胎儿无创产前检测的系统
US11773443B2 (en) 2014-05-09 2023-10-03 Eurofins Lifecodexx Gmbh Multiplex detection of DNA that originates from a specific cell-type
WO2025113619A1 (en) * 2023-11-29 2025-06-05 Centre For Novostics Enrichment of clinically-relevant nucleic acids

Families Citing this family (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US7888127B2 (en) 2008-01-15 2011-02-15 Sequenom, Inc. Methods for reducing adduct formation for mass spectrometry analysis
CA3209502A1 (en) * 2008-09-16 2010-03-25 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8476013B2 (en) * 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US9926593B2 (en) 2009-12-22 2018-03-27 Sequenom, Inc. Processes and kits for identifying aneuploidy
AU2011255641A1 (en) 2010-05-18 2012-12-06 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
JP6153874B2 (ja) 2011-02-09 2017-06-28 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
US8980553B2 (en) * 2011-04-02 2015-03-17 New England Biolabs, Inc. Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
US8455221B2 (en) 2011-04-29 2013-06-04 Sequenom, Inc. Quantification of a minority nucleic acid species
US20140235474A1 (en) 2011-06-24 2014-08-21 Sequenom, Inc. Methods and processes for non invasive assessment of a genetic variation
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2013052913A2 (en) 2011-10-06 2013-04-11 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20140242588A1 (en) 2011-10-06 2014-08-28 Sequenom, Inc Methods and processes for non-invasive assessment of genetic variations
US8688388B2 (en) 2011-10-11 2014-04-01 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
DK2766496T3 (en) 2011-10-11 2017-05-15 Sequenom Inc METHODS AND PROCESSES FOR NON-INVASIVE ASSESSMENT OF GENETIC VARIATIONS
EP2805280B1 (en) 2012-01-20 2022-10-05 Sequenom, Inc. Diagnostic processes that factor experimental conditions
HK1206055A1 (en) 2012-03-02 2015-12-31 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2852680B1 (en) 2012-05-21 2019-12-25 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2014011928A1 (en) 2012-07-13 2014-01-16 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2904534B1 (en) 2012-10-04 2021-12-15 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20130189684A1 (en) 2013-03-12 2013-07-25 Sequenom, Inc. Quantification of cell-specific nucleic acid markers
EP2971100A1 (en) 2013-03-13 2016-01-20 Sequenom, Inc. Primers for dna methylation analysis
US9305756B2 (en) 2013-03-13 2016-04-05 Agena Bioscience, Inc. Preparation enhancements and methods of use for MALDI mass spectrometry
ES2939547T3 (es) 2013-04-03 2023-04-24 Sequenom Inc Métodos y procedimientos para la evaluación no invasiva de variaciones genéticas
IL300163B2 (en) 2013-05-24 2024-06-01 Sequenom Inc Methods and processes for non-invasive assessment of genetic variations
MX2015016911A (es) 2013-06-21 2016-06-21 Sequenom Inc Metodos y procesos para evaluacion no invasiva de variaciones geneticas.
IL289974B (en) 2013-10-04 2022-09-01 Sequenom Inc Methods and processes for non-invasive evaluation of genetic variations
EP3055427B1 (en) 2013-10-07 2018-09-12 Sequenom, Inc. Methods and processes for non-invasive assessment of chromosome alterations
EP3736344A1 (en) 2014-03-13 2020-11-11 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
JP6659575B2 (ja) 2014-04-21 2020-03-04 ナテラ， インコーポレイテッド 変異の検出および染色体分節の倍数性
EP2942401A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Detection of DNA that originates from a specific cell-type
EP3598452B1 (en) 2014-05-30 2023-07-26 Sequenom, Inc. Chromosome representation determinations
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
EP3760739B1 (en) 2014-07-30 2025-09-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
JP6971845B2 (ja) 2014-10-10 2021-11-24 セクエノム， インコーポレイテッド 遺伝子の変動の非侵襲的評価のための方法および処理
EP3018213A1 (en) 2014-11-04 2016-05-11 Genesupport SA Method for determining the presence of a biological condition by determining total and relative amounts of two different nucleic acids
WO2016179530A1 (en) * 2015-05-06 2016-11-10 Seracare Life Sciences, Inc. Liposomal preparations for non-invasive-prenatal or cancer screening
DK3294906T3 (en) 2015-05-11 2024-08-05 Natera Inc Methods for determining ploidy
JP6643815B2 (ja) * 2015-05-15 2020-02-12 森永乳業株式会社 腸内状態の判定方法、および腸内状態の判定装置
EP3375886A4 (en) * 2015-11-10 2018-09-19 Fujifilm Corporation Method for determining whether cells or cell masses are originated from same person, or unrelated persons, or parent and child, or related persons
CN109477138A (zh) 2016-04-15 2019-03-15 纳特拉公司 肺癌检测方法
EP3464626B1 (en) 2016-05-27 2022-04-06 Sequenom, Inc. Methods for detecting genetic variations
JP7048105B2 (ja) 2016-07-15 2022-04-05 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 核酸ライブラリを生成する方法
CA3030890A1 (en) 2016-07-27 2018-02-01 Sequenom, Inc. Genetic copy number alteration classifications
WO2018022906A1 (en) 2016-07-27 2018-02-01 Sequenom, Inc. Methods for non-invasive assessment of genomic instability
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
EP3571615B1 (en) 2017-01-20 2024-01-24 Sequenom, Inc. Methods for non-invasive assessment of genetic alterations
CA3049455C (en) 2017-01-20 2023-06-13 Sequenom, Inc. Sequencing adapter manufacture and use
CA3194621A1 (en) 2017-01-20 2018-07-26 Sequenom, Inc. Methods for non-invasive assessment of copy number alterations
JP7237003B2 (ja) 2017-01-24 2023-03-10 セクエノム， インコーポレイテッド 遺伝子片の評価のための方法およびプロセス
US12421550B2 (en) 2017-03-17 2025-09-23 Sequenom, Inc. Methods and processes for assessment of genetic mosaicism
US12084720B2 (en) 2017-12-14 2024-09-10 Natera, Inc. Assessing graft suitability for transplantation
CN111954720A (zh) 2018-01-12 2020-11-17 克拉雷特生物科学有限责任公司 用于分析核酸的方法和组合物
CN112236535A (zh) 2018-04-14 2021-01-15 纳特拉公司 用于借助于循环肿瘤dna的个人化检测的癌症检测和监测的方法
AU2019280712B2 (en) 2018-06-06 2025-11-20 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
EP3874060B1 (en) * 2018-10-31 2024-10-16 Guardant Health, Inc. Method and system for calibrating epigenetic partitioning assays
CN114174530A (zh) 2019-04-05 2022-03-11 克拉雷特生物科学有限责任公司 用于分析核酸的方法和组合物
EP3973081A4 (en) * 2019-05-23 2022-09-07 American Molecular Laboratories, Inc. METHODS FOR DETECTING RARE DNA SEQUENCES IN FECAL SAMPLES
EP3980559A1 (en) 2019-06-06 2022-04-13 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
CN114929893A (zh) * 2019-06-24 2022-08-19 齐罗马科德公司 差异定量核酸的方法
AU2020363787A1 (en) 2019-10-09 2022-04-21 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
EP4052259A1 (en) 2019-10-31 2022-09-07 Sequenom, Inc. Application of mosaicism ratio in multifetal gestations and personalized risk assessment
TW202146659A (zh) * 2020-05-28 2021-12-16 美商伊路米納有限公司 比較具有不同特徵的多核苷酸拷貝
US20230235320A1 (en) 2020-06-24 2023-07-27 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
US20240395358A1 (en) 2020-10-08 2024-11-28 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
IL302988A (en) * 2020-11-19 2023-07-01 Nucleix Ltd Detection of methylation changes in DNA samples using restriction enzymes and high-throughput sequencing
US20240023862A1 (en) 2021-01-12 2024-01-25 Ricoh Company, Ltd. Diagnostic support apparatus, diagnostic support method, and diagnostic support program
WO2024186778A1 (en) 2023-03-03 2024-09-12 Laboratory Corporation Of America Holdings Methods and systems for positive cfdna screening on genetic variations using mosaicism ratio
CN121153082A (zh) 2023-05-15 2025-12-16 美国控股实验室公司 用于在全基因组测序中进行泛癌筛查的机器学习方法

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2187108A (en) 1938-05-27 1940-01-16 Du Pont Process of purifying lead nitrate solutions
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
ATE267877T1 (de) 1993-01-07 2004-06-15 Sequenom Inc Dns - sequenzierung durch massenspektronomie
WO1997037041A2 (en) 1996-03-18 1997-10-09 Sequenom, Inc. Dna sequencing by mass spectrometry
US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
GB9704444D0 (en) 1997-03-04 1997-04-23 Isis Innovation Non-invasive prenatal diagnosis
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
DE60031540T2 (de) 1999-06-28 2007-05-16 California Institute Of Technology, Pasadena Mikromechanische pump- und ventilsysteme
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
US6927028B2 (en) 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
WO2003062441A1 (en) * 2002-01-18 2003-07-31 Genzyme Corporation Methods for fetal dna detection and allele quantitation
WO2004022721A2 (en) 2002-09-06 2004-03-18 The Trustees Of Boston University Quantification of gene expression
AU2004217872B2 (en) * 2003-03-05 2010-03-25 Genetic Technologies Limited Identification of fetal DNA and fetal cell markers in maternal plasma or serum
EP1641809B2 (en) 2003-07-05 2018-10-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
ATE425265T1 (de) 2003-07-31 2009-03-15 Sequenom Inc Verfahren für multiplex- polymerasekettenreaktionen auf hohem niveau und homogenen massenverlängerungsreaktionen zur genotypisierung von polymorphismen
EP1664077B1 (en) 2003-09-05 2016-04-13 Trustees of Boston University Method for non-invasive prenatal diagnosis
WO2005035725A2 (en) * 2003-10-08 2005-04-21 The Trustees Of Boston University Methods for prenatal diagnosis of chromosomal abnormalities
DE60328193D1 (de) 2003-10-16 2009-08-13 Sequenom Inc Nicht invasiver Nachweis fötaler genetischer Merkmale
CN101243191B (zh) * 2004-11-29 2014-04-16 塞昆纳姆股份有限公司 用于检测甲基化dna的手段和方法
EP1859050B1 (en) 2005-03-18 2012-10-24 The Chinese University Of Hong Kong A method for the detection of chromosomal aneuploidies
EP2385143B1 (en) 2006-02-02 2016-07-13 The Board of Trustees of the Leland Stanford Junior University Non-invasive fetal genetic screening by digital analysis
US7754428B2 (en) * 2006-05-03 2010-07-13 The Chinese University Of Hong Kong Fetal methylation markers
US7901884B2 (en) * 2006-05-03 2011-03-08 The Chinese University Of Hong Kong Markers for prenatal diagnosis and monitoring
US8679741B2 (en) 2006-05-31 2014-03-25 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
WO2009035447A1 (en) * 2006-06-14 2009-03-19 Living Microsystems, Inc. Diagnosis of fetal abnormalities by comparative genomic hybridization analysis
WO2008157264A2 (en) 2007-06-15 2008-12-24 Sequenom, Inc. Combined methods for the detection of chromosomal aneuploidy
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
US8476013B2 (en) * 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
CA3209502A1 (en) * 2008-09-16 2010-03-25 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
JP6447765B1 (ja) * 2017-11-21 2019-01-09 株式会社リコー 検査デバイス及びデバイス

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10093919B2 (en) 2007-10-31 2018-10-09 Akonni Biosystems, Inc. Method and kit for purifying nucleic acids
CN105164509B (zh) * 2012-08-28 2018-02-23 阿科尼生物系统公司 用于纯化核酸的方法和试剂盒
CN105164509A (zh) * 2012-08-28 2015-12-16 阿科尼生物系统公司 用于纯化核酸的方法和试剂盒
CN112037860A (zh) * 2013-06-13 2020-12-04 阿瑞奥萨诊断公司 用于非入侵性性染色体非整倍性确定的统计分析
CN112037860B (zh) * 2013-06-13 2024-02-23 豪夫迈·罗氏有限公司 用于非入侵性性染色体非整倍性确定的统计分析
US11773443B2 (en) 2014-05-09 2023-10-03 Eurofins Lifecodexx Gmbh Multiplex detection of DNA that originates from a specific cell-type
CN107223159A (zh) * 2014-05-09 2017-09-29 科戴克斯生命股份公司 源自特定细胞类型的dna的检测及相关方法
US11965207B2 (en) 2014-05-09 2024-04-23 Eurofins Lifecodexx Gmbh Detection of DNA that originates from a specific cell-type and related methods
CN109689896A (zh) * 2015-11-10 2019-04-26 科戴克斯生命股份公司 使用在胎儿和怀孕雌性动物之间差异甲基化的dna区域检测胎儿染色体非整倍性
US11753684B2 (en) 2015-11-10 2023-09-12 Eurofins Lifecodexx Gmbh Detection of fetal chromosomal aneuploidies using DNA regions that are differentially methylated between the fetus and the pregnant female
CN110734907A (zh) * 2019-09-17 2020-01-31 上海凌恩生物科技有限公司 一种叶绿体dna的富集方法
CN111755072A (zh) * 2020-08-04 2020-10-09 深圳吉因加医学检验实验室 一种同时检测甲基化水平、基因组变异和插入片段的方法及装置
CN116246704B (zh) * 2023-05-10 2023-08-15 广州精科生物技术有限公司 用于胎儿无创产前检测的系统
CN116246704A (zh) * 2023-05-10 2023-06-09 广州精科生物技术有限公司 用于胎儿无创产前检测的系统
WO2025113619A1 (en) * 2023-11-29 2025-06-05 Centre For Novostics Enrichment of clinically-relevant nucleic acids

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