CN102177809A - Method for carrying out uniform selenium-enriched cultivation of pleurotus geesteranus by using selenium yeast - Google Patents
Method for carrying out uniform selenium-enriched cultivation of pleurotus geesteranus by using selenium yeast Download PDFInfo
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Abstract
The invention discloses a method for carrying out uniform selenium-enriched cultivation of pleurotus geesteranus by using selenium yeast, which is characterized by comprising steps of mixing a selenium-enriched cultivation material additive of which the weight is 1-10 percent of the total weight of a cultivation drier into the cultivation drier and carrying out cultivation according to a common cultivation method to obtain the uniform selenium-enriched pleurotus geesteranus, wherein the total content of selenium in the selenium-enriched cultivation material additive is in the range of 5-200mg/kg; the diameters of particles in the selenium-enriched cultivation material additive are distributed in a multiple diameter region mode in the range of 0.005-5.0mm; the weight of the particles with the diameter in the range of 1.0-5.0mm is at least 10 percent of the total weight of the additive; the weight of the particles with the diameter in the range of 0.005-0.1mm is at least 40 percent of the total weight of the additive; and the selenium-enriched cultivation material additive comprises the following composition raw materials in parts by weight: 100 parts of maize cob meal and 0.45-18 parts of selenium yeast. The pleurotus geesteranus obtained by using the method has high selenium utilization rate and stable selenium content between batches. Moreover, the method has no hidden danger of selenium pollution and is convenient to operate and easy to popularize.
Description
Technical field
The present invention relates to edible mushroom, particularly a kind of is that selenium source carries out the selenium nutrition-intensified method of elegant precious mushroom with the selenium yeast, belongs to the conversion technology field of selenium resource.
Background technology
Elegant precious mushroom (Pleurotus geesteranus) belongs to Eumycota, Basidiomycetes, Agaricales, Pleurotaceae Pleurotus on taxonomy.Elegant precious mushroom quality is tender and crisp, fresh and sweet tasty and refreshing, has happy crab fragrance, and is nutritious, has higher edible and medical value, is the strain excellent in the oyster cap fungus.It is a kind of high protein, low-fat nutraceutical, and its protein content approaches meat, than the high 3-6 of general vegetables doubly, and its quality delicacy, fiber content is few; More valuablely be, it contains ethnic group self and can not make, and the threonine that lacks usually in the food, lysine, homo amino acid etc.This kind edible mushroom is gained the name because of delicate and precious, and its shape is small and exquisite, and is delicious in taste, very popular, and in very great demand in the international market, DEVELOPMENT PROSPECT is wide.
Selenium is a kind of humans and animals trace elements necessary, have remove free radical anti-oxidant (, delay senility, biological propertyes such as anticancer, enhancing immunity, antagonism heavy metal.It is 50～250 μ g that a day selenium intake is recommended by Chinese Soclety of Nutrition.Human body selenium source is main to be taken in by meals, but China adult's average day selenium intake only have about 36 μ g, and therefore, the selemium nutrition condition of poor is at China's ubiquity.The data that WHO announces shows that also the whole world has more than 40 country to belong to low selenium or scarce selenium area.Selenium mainly is divided into inorganic selenium and organic selenium two big classes: the former comprises selenate, selenite etc., and inorganic selenium assimilation effect for humans and animals is poor, and risk of toxicity is big, and improper use very easily causes selenosis; The latter mainly comprises forms such as seleno-amino acids, seleno albumen, selenium polysaccharide, good absorbing effect concerning humans and animals, and toxicity is little, is more suitable for mending the requirement of selenium.
Rich selenium agricultural byproducts are human body Selenium Supplement element effective ways, and therefore, technique of enriched selenium is scientists study hot of research and development field.The nutritionist points out that except that dietary fiber, edible mushroom contains the complete nutrients matter of needed by human body, and an adult eats the 100g edible mushroom every day, can obtain the 15-17g high-quality protein; Can obtain 600mg calcium, be equivalent to 80% of daily requirement; Can satisfy 11% of human body amphemera requirement, but Se content is lower than 5g/kg in the general edible mushroom.Existing edible mushroom selenium-enriching cultivation technology is mainly directly adds the selenium salting liquid in cultivate material, method is simple, cost is lower for this, but inorganic residual quantity is big, toxicity big, absorption difference, and edible mushroom is low to se use efficiency in the cultivate material, and major part rests on laboratory or small test stage; And whole growth in the cycle fruit body selenium content whether stable, promptly can the Se content of different batches fruit body keep stablizing on the knees of the gods.
Summary of the invention
Problems such as the object of the invention is to provide the even selenium-enriching cultivation method of the precious mushroom of a kind of show, and it is batch inhomogeneous to have solved in the prior art behind the selenium-enriching cultivation between the growth of elegant precious mushroom head tide or many tide growth elegant each strains of precious mushroom in back Se content, and the rich selenium amount fluctuation of fruit body is big.
In order to solve these problems of the prior art, technical scheme provided by the invention is:
A kind of method of utilizing selenium yeast to carry out the precious mushroom of even selenium-enriching cultivation show, it is characterized in that described method is included in cultivation and sneaks into the selenium-enriching cultivation feed additives that accounts for cultivation siccative gross weight～10%, the step of cultivating the elegant precious mushroom that obtains even rich selenium according to the common cultivation method in the siccative; Wherein to contain the selenium total amount be 5～200mg/kg to the selenium-enriching cultivation feed additives, and grain diameter is many particle size region and distributes in the selenium-enriching cultivation feed additives in 0.005～5.0mm scope, and particle diameter is that the particle of 1.0～5.0mm accounts for 10% of additive gross weight at least; Particle diameter is that the particle of 0.005～0.1mm accounts for 40% of additive gross weight at least; The component raw material that described selenium-enriching cultivation feed additives adopts comprises in parts by weight separately:
Maize cob meal 100 parts by weight;
Selenium yeast 0.45～18 parts by weight.
Preferably, grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10～20% of 1.0～5.0mm;
Particle diameter is the particle 40～70% of 0.005～0.1mm;
The particle 20～40% of other particle size range.
Preferably, grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10% of 1.0～5.0mm;
Particle diameter is the particle 50% of 0.005～0.1mm;
The particle 40% of other particle size range.
Preferably, grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 20% of 1.0～5.0mm;
Particle diameter is the particle 50% of 0.005～0.1mm;
The particle 30% of other particle size range.
Preferably, described cultivation siccative comprises with the weight percent meter of its component:
Corncob 75%;
Cotton seed hulls 20%;
Quicklime 3%;
Land plaster 1%;
Composite fertilizer 1%.
Preferably, described common cultivation method comprises that the cultivation siccative that will sneak into the selenium-enriching cultivation feed additives adds running water according to material-water ratio, abundant stirring and evenly mixing, heap fermentation at 1: 1.3, the steam sterilization sterilization treatment is used in the pack back, makes the even selenium enriched oyster mushroom of final composts or fertilisers of cultivating inoculated and cultured.
Preferably, the maize cob meal that uses of described selenium-enriching cultivation feed additives is pulverized as natural crops offcuts corncob and is formed.
Preferably, the particle of described selenium-enriching cultivation feed additives is by finishing granulation process after maize cob meal and the selenium yeast raw material pulverizing.
Preferably, during the cultivation of the medicine that is used to keep healthy food selenium enriched oyster mushroom, the Se content of the selenium-enriching cultivation feed additives of use is 100～200mg/kg; When being used for the cultivation of daily edible selenium enriched oyster mushroom, the Se content of the selenium-enriching cultivation feed additives of use is 5～50mg/kg.
Preferably, grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10% of 1.0～5.0mm;
Particle diameter is the particle 60% of 0.005～0.1mm;
The particle 30% of other particle size range.
Preferably, grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10% of 1.0～5.0mm;
Particle diameter is the particle 70% of 0.005～0.1mm;
The particle 20% of other particle size range.
Wherein A～B% represents scope in A% arrives the B% scope, and comprises B% and A%.
The inventor is through a large amount of research and test, combine many tide growths of elegant precious mushroom and the different characteristics of each tide growth amount of gathering, by preparing a kind of different proportioning features of different grain size that have, discharge the specialist additive of selenium wherein with different speed, providing a kind of is that selenium source carries out the selenium nutrition-intensified method of elegant precious mushroom with the selenium yeast, realizes the stability of rich selenium amount between each damp mushroom and to the high usage of selenium resource and the high conversion ratio that absorbs.
The inventive method se use efficiency height, no selenium contamination hidden danger, are easy to promote and can realize safe and reliable in the product, stable and controllable handled easily, realize that elegant precious mushroom is selenium nutrition-intensified.Below be concrete operations step of the present invention:
At first prepare a kind of selenium enriched oyster mushroom cultivate material additive:
1. with a kind of natural crops offcuts, corncob by screening, dry, to be crushed to particle diameter be 0.005～0.1mm, designs the prescription of the required cultivate material additive of the precious mushroom of cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals
0.45～18 part of selenium yeast;
2. above-mentioned selenium yeast is the dark friendly selenium yeast in Zhejiang, and average grain diameter is 0.1mm, and total Se content is 2000mg/kg; With itself and the abundant stirring and evenly mixing of maize cob meal, make particle diameter below 0.1mm, selenium content is compound in 5～500mg/kg scope.
3. above-mentioned compound processing granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.005～0.1mm, 0.1～1.0mm, the 1.0～5.0mm scope.
4. above-mentioned three kinds of particle diameters are respectively: the particle of 0.005～0.1mm, 0.1～1.0mm, 1.0～5.0mm mixes by following percentage by weight: 40～70%, 20～40%, 10～20%, promptly obtain having the selenium-enriching cultivation feed additives of different grain size proportioning feature, its total selenium content is still in the 5-200mg/kg scope.
According to the difference of required selenium enriched oyster mushroom product purpose, select the different cultivate material additive of selenium content during use.For example, the selenium enriched oyster mushroom cultivate material additive in 5～50mg/kg scope is selected in daily edible rich selenium mushroom cultivation; The health care medicine is eaten rich selenium mushroom cultivation and is selected the interior cultivate material additive of 100～200mg/kg scope.
The basis cultivate material is formed: corncob 75%, cotton seed hulls 20%, quicklime 3%, land plaster 1%, composite fertilizer 1%.During the cultivate material spice, the ratio in 1～10% is sneaked into basic cultivate material with additive, and stirring and evenly mixing makes in the cultivate material final selenium content in 0.5～20mg/kg scope.
Add running water by material-water ratio 1: 1.3 and fully stir, mixing once more, heap fermentation, the steam sterilization sterilization treatment is used in the pack back, makes final composts or fertilisers of cultivating inoculated and cultured selenium enriched oyster mushroom; Also can directly adopt the cultivation in raw material mode to produce the selenium enriched oyster mushroom.
When the precious massee fruiting bodies of show was gathered, sampling detected wherein Se content, and bright sample Se content improves 6～240 times than the precious mushroom of common cultivation show, can reach 0.2～8.5mg/kg.And organic selenium content significantly increases in the fruit body, and wherein the selenium that exists with various seleno-amino acids forms accounts for total selenium more than 85%.
Statistics output and se use efficiency in the damp mushroom recovery process, the result shows: selenium nutrition-intensified group is improved 20% than common cultivation group head tide mushroom output, and se use efficiency is up to 40%.
In the whole growth of fruit body in the cycle, the relative standard deviation RSD% of its selenium content can be strict controlled in 15%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD% is higher than 30%) edible mushroom of the additive cultivation of different grain size proportioning feature; Selenium content is more stable in the same stage fruit body, and RSD all is lower than 5%.
Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are marked.The result: the selenium enriched oyster mushroom exceeds 5 fens than the precious mushroom of common show.
Utilize the present invention can obtain rich selenium better effects if, organic selenium content is higher, and the elegant precious mushroom that rich selenium quality is more stable promotes elegant precious mushroom product and is high-quality selenium-enriched health care product, is that daily delicacies can be used as the dietotherapy good merchantable brand again, and the people that are more convenient for accept.
With respect to scheme of the prior art, advantage of the present invention is:
(1) the cultivate material additive for preparing among the present invention is the selenium-enriched edible mushroom specialist additive, combines the edible fungi growth characteristics, has different grain size proportioning feature, can realize that selenium wherein discharges with different speed, thereby realizes the stability of rich selenium amount between each damp mushroom.
(2) yeast selenium itself just has selenium form safety, characteristics that bioavailability is high, more utilizes elegant precious mushroom that it is absorbed; Maize cob meal can prevent effectively that as a kind of selenium source carrier selenium source runs off and the even distribution of assurance selenium source.
(3) the present invention realizes the stability of additive quality by steps such as elementary compound preparation, granulation and each granularity particle mix in varing proportions; Realize mixing once more by spice before the cultivate material fermentation, this just guarantees the even distribution of selenium resource in cultivate material, is beneficial to the even absorption to selenium of mycelium and fruit body.
(4) by additive and common cultivation material by a certain percentage (1～10%) mix, make the cultivate material of final selenium content in 0.5～20mg/kg scope, can make that the selenium resource is absorbed and used better in the cultivate material, organic selenium content significantly improves in the fruit body.Testing result shows that selenomethionine is the main occurrence patterns of selenium in the elegant precious mushroom, and this form selenium accounts for 85～89% of total Se content.
(5) use the selenium enriched oyster mushroom that the present invention produces, bright mushroom selenium content can be strict controlled between 0.2～2.5mg/kg and the 3～8.5mg/kg, and the former can be for daily edible, and the latter can be used as food therapeutic health.
(6) statistics output and se use efficiency in the damp mushroom recovery process, the result shows: selenium nutrition-intensified group is improved more than 20% than common cultivation group head tide mushroom output; Se use efficiency is higher than the selenium enriched oyster mushroom that utilizes the cultivation of inorganic selenium salt under the equal conditions far away up to 40%.
(7) in the whole growth of fruit body in the cycle, the relative standard deviation RSD of its selenium content can be strict controlled in 15%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) edible mushroom of the additive cultivation of different grain size proportioning feature.
(8) to be suitable for any material be the elegant precious mushroom cultivation of cultivation base-material to the additive rich in selenium of the present invention preparation, as sawmilling end, wheat bran, corn tangerine bar, corncob, rape straw, cotton seed hulls etc.
Embodiment
Below in conjunction with specific embodiment such scheme is described further.Should be understood that these embodiment are used to the present invention is described and are not limited to limit the scope of the invention.The implementation condition that adopts among the embodiment can be done further adjustment according to the condition of concrete producer, and not marked implementation condition is generally the condition in the normal experiment.
Embodiment 1 elegant precious mushroom selenium-enriching cultivation embodiment
(1) preparation of selenium enriched oyster mushroom additive:
1. with a kind of natural crops offcuts, corncob by screening, dry, to be crushed to particle diameter be 0.005～0.1mm, the prescription of the required cultivate material additive of the precious mushroom of design cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals;
0.45～0.9 part of selenium yeast.
2. above-mentioned selenium yeast is the dark friendly selenium yeast in Zhejiang, and average grain diameter is 0.1mm, and total Se content is 2000mg/kg; It is crushed to after particle diameter is 0.005～0.1mm, with the abundant stirring and evenly mixing of maize cob meal, promptly makes particle diameter below 0.1mm, selenium content is compound in 5～10mg/kg scope.
3. above-mentioned compound processing granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.005～0.1mm, 0.1～1.0mm, the 1.0～5.0mm scope.
4. above-mentioned three kinds of size particles are mixed by following percentage by weight: 70%, 20%, 10%, promptly obtain having different grain size proportioning feature, selenium content is the selenium-enriching cultivation feed additives of 5～10mg/kg.
(2) elegant precious mushroom is selenium nutrition-intensified:
In certain edible mushroom base, select additive for use as preparation as described in (1); The common cultivation material is formed: corncob 75%, cotton seed hulls 20%, quicklime 3%, land plaster 1%, composite fertilizer 1%.When the cultivate material spice, ratio in 10% and common cultivation material mixing, making final selenium content is 0.5～1.0mg/kg; Press material-water ratio 1: 1.3 and add running water, fully stir, mixing once more, heap fermentation, the steam sterilization sterilization treatment use in the pack back, makes final composts or fertilisers of cultivating inoculation, a bacterium; Cultivate according to precious mushroom cultivation of normal show and management method.
(3) implementation result:
Selenium nutrition-intensified group of head tide mushroom production ratio common cultivation group output improves 20%, and se use efficiency is up to 40%; Total Se content is about 0.2～0.5mg/kg in the selenium enriched oyster massee fruiting bodies, is 6～15 times of common cultivation group, and wherein the selenium that exists with various seleno-amino acids forms accounts for more than 85% of total selenium; Fruit body is in whole growth in the cycle, the relative standard deviation RSD of selenium content can be strict controlled in 15%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) elegant precious mushroom of the additive cultivation of different grain size proportioning feature.Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are marked.The result: the selenium enriched oyster mushroom exceeds 5 fens than the precious mushroom of common show.
Embodiment 2 elegant precious mushroom selenium-enriching cultivation embodiment
(1) preparation of selenium enriched oyster mushroom additive:
1. with a kind of natural crops offcuts, corncob by screening, dry, to be crushed to particle diameter be 0.005～0.1mm, the prescription of the required cultivate material additive of the precious mushroom of design cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals;
0.9～4.5 part of selenium yeast.
2. above-mentioned selenium yeast is the dark friendly selenium yeast in Zhejiang, and average grain diameter is 0.1mm, and total Se content is 2000mg/kg; It is crushed to after particle diameter is 0.005～0.1mm, with the abundant stirring and evenly mixing of maize cob meal, promptly makes particle diameter below 0.1mm, selenium content is compound in 10～50mg/kg scope.
3. above-mentioned compound processing granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.005～0.1mm, 0.1～1.0mm, the 1.0～5.0mm scope.
4. above-mentioned three kinds of size particles are mixed by following percentage by weight: 60%, 30%, 10%, promptly obtain having different grain size proportioning feature, selenium content is the selenium-enriching cultivation feed additives of 10～50mg/kg.
(2) elegant precious mushroom is selenium nutrition-intensified:
In certain edible mushroom base, select additive for use as preparation as described in (1); The common cultivation material is formed: corncob 75%, cotton seed hulls 20%, quicklime 3%, land plaster 1%, composite fertilizer 1%.When the cultivate material spice, ratio in 10% and common cultivation material mixing, making final selenium content is 1～5mg/kg; Press material-water ratio 1: 1.3 and add running water, fully stir, mixing once more, heap fermentation, the steam sterilization sterilization treatment use in the pack back, makes final composts or fertilisers of cultivating inoculation, a bacterium; Cultivate according to precious mushroom cultivation of normal show and management method.
(3) implementation result:
Selenium nutrition-intensified group of head tide mushroom production ratio common cultivation group output improves 20%, and se use efficiency is up to 40%; Total Se content is 0.5～2.5mg/kg in the selenium enriched oyster massee fruiting bodies, is 15～70 times of common cultivation group, and wherein the selenium that exists with various seleno-amino acids forms accounts for more than 85% of total selenium; Fruit body is in whole growth in the cycle, the relative standard deviation RSD of selenium content can be strict controlled in 15%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) elegant precious mushroom of the additive cultivation of different grain size proportioning feature.Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are marked.The result: the selenium enriched oyster mushroom exceeds 5 fens than the precious mushroom of common show.
Embodiment 3 elegant precious mushroom selenium-enriching cultivation embodiment
(1) preparation of selenium enriched oyster mushroom additive:
1. with a kind of natural crops offcuts, corncob by screening, dry, to be crushed to particle diameter be 0.005～0.1mm, the prescription of the required cultivate material additive of the precious mushroom of design cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals;
4.5～9 parts of selenium yeasts.
2. above-mentioned selenium yeast is the dark friendly selenium yeast in Zhejiang, and average grain diameter is 0.1mm, and total Se content is 2000mg/kg; It is crushed to after particle diameter is 0.005～0.1mm, with the abundant stirring and evenly mixing of maize cob meal, promptly makes particle diameter below 0.1mm, selenium content is compound in 50～100mg/kg scope.
3. above-mentioned compound processing granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.005～0.1mm, 0.1～1.0mm, the 1.0～5.0mm scope.
4. above-mentioned three kinds of size particles are mixed by following percentage by weight: 60%, 20%, 20%, promptly obtain having different grain size proportioning feature, selenium content is the selenium-enriching cultivation feed additives of 50～100mg/kg.
(2) elegant precious mushroom is selenium nutrition-intensified:
In certain edible mushroom base, select additive for use as preparation as described in (1); The common cultivation material is formed: corncob 75%, cotton seed hulls 20%, quicklime 3%, land plaster 1%, composite fertilizer 1%.When the cultivate material spice, ratio in 5% and common cultivation material mixing, making final selenium content is 2.5～5mg/kg; Press material-water ratio 1: 1.3 and add running water, fully stir, mixing once more, heap fermentation, the steam sterilization sterilization treatment use in the pack back, makes final composts or fertilisers of cultivating inoculation, a bacterium; Cultivate according to precious mushroom cultivation of normal show and management method.
(3) implementation result:
Selenium nutrition-intensified group of head tide mushroom production ratio common cultivation group output improves 20%, and se use efficiency is up to 40%; Total Se content is about 1.0～2.5mg/kg in the selenium enriched oyster massee fruiting bodies, is 25～70 times of common cultivation group, and wherein the selenium that exists with various seleno-amino acids forms accounts for more than 85% of total selenium; Fruit body is in whole growth in the cycle, the relative standard deviation RSD of selenium content can be strict controlled in 15%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) elegant precious mushroom of the additive cultivation of different grain size proportioning feature.Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are marked.The result: the selenium enriched oyster mushroom exceeds 5 fens than the precious mushroom of common show.
Embodiment 4 elegant precious mushroom selenium-enriching cultivation embodiment
(1) preparation of selenium enriched oyster mushroom additive:
1. with a kind of natural crops offcuts, corncob by screening, dry, to be crushed to particle diameter be 0.005～0.1mm, the prescription of the required cultivate material additive of the precious mushroom of design cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals;
9～18 parts of selenium yeasts.
2. above-mentioned selenium yeast is the dark friendly selenium yeast in Zhejiang, and average grain diameter is 0.1mm, and total Se content is 2000mg/kg; It is crushed to after particle diameter is 0.005～0.1mm, with the abundant stirring and evenly mixing of maize cob meal, promptly makes particle diameter below 0.1mm, selenium content is compound in 100～200mg/kg scope.
3. above-mentioned compound processing granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.005～0.1mm, 0.1～1.0mm, the 1.0～5.0mm scope.
4. above-mentioned three kinds of size particles are mixed by following percentage by weight: 50%, 40%, 10%, promptly obtain having different grain size proportioning feature, total selenium content is the selenium-enriching cultivation feed additives of 100～200mg/kg.
(2) elegant precious mushroom is selenium nutrition-intensified:
In certain edible mushroom base, select additive for use as preparation as described in (1); The common cultivation material is formed: corncob 75%, cotton seed hulls 20%, quicklime 3%, land plaster 1%, composite fertilizer 1%.When the cultivate material spice, ratio in 10% and common cultivation material mixing, making final selenium content is 10～20mg/kg; Press material-water ratio 1: 1.3 and add running water, fully stir, mixing once more, heap fermentation, the steam sterilization sterilization treatment use in the pack back, makes final composts or fertilisers of cultivating inoculation, a bacterium; Cultivate according to precious mushroom cultivation of normal show and management method.
(3) implementation result:
Selenium nutrition-intensified group of head tide mushroom production ratio common cultivation group output improves 20%, and se use efficiency is up to 40%; Total Se content is 3～8.5mg/kg in the selenium enriched oyster massee fruiting bodies, is 85～240 times of common cultivation group, and wherein the selenium that exists with various seleno-amino acids forms accounts for more than 85% of total selenium; Fruit body is in whole growth in the cycle, the relative standard deviation RSD of selenium content can be strict controlled in 15%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) elegant precious mushroom of the additive cultivation of different grain size proportioning feature.Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are marked.The result: the selenium enriched oyster mushroom exceeds 5 fens than the precious mushroom of common show.
Above-mentioned example only is explanation technical conceive of the present invention and characteristics, and its purpose is to allow the people who is familiar with this technology can understand content of the present invention and enforcement according to this, can not limit protection scope of the present invention with this.All spirit essence is done according to the present invention equivalent transformation or modification all should be encompassed within protection scope of the present invention.
Claims (9)
1. method of utilizing selenium yeast to carry out the precious mushroom of even selenium-enriching cultivation show, it is characterized in that described method is included in cultivation and sneaks into the selenium-enriching cultivation feed additives that accounts for cultivation siccative gross weight～10%, the step of cultivating the elegant precious mushroom that obtains even rich selenium according to the common cultivation method in the siccative; Wherein to contain the selenium total amount be 5～200mg/kg to the selenium-enriching cultivation feed additives, and grain diameter is many particle size region and distributes in the selenium-enriching cultivation feed additives in 0.005～5.0mm scope, and particle diameter is that the particle of 1.0～5.0mm accounts for 10% of additive gross weight at least; Particle diameter is that the particle of 0.005～0.1mm accounts for 40% of additive gross weight at least; The component raw material that described selenium-enriching cultivation feed additives adopts comprises in parts by weight separately:
Maize cob meal 100 parts by weight;
Selenium yeast 0.45～18 parts by weight.
2. method according to claim 1 is characterized in that grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10～20% of 1.0～5.0mm;
Particle diameter is the particle 40～70% of 0.005～0.1mm;
The particle 20～40% of other particle size range.
3. method according to claim 2 is characterized in that grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10% of 1.0～5.0mm;
Particle diameter is the particle 50% of 0.005～0.1mm;
The particle 40% of other particle size range.
4. method according to claim 2 is characterized in that grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005～5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 20% of 1.0～5.0mm;
Particle diameter is the particle 50% of 0.005～0.1mm;
The particle 30% of other particle size range.
5. method according to claim 1 is characterized in that described cultivation siccative comprises with the weight percent meter of its component:
Corncob 75%;
Cotton seed hulls 20%;
Quicklime 3%;
Land plaster 1%;
Composite fertilizer 1%.
6. method according to claim 1, it is characterized in that described common cultivation method comprises that the cultivation siccative of will sneak into the selenium-enriching cultivation feed additives adds running water according to material-water ratio at 1: 1.3, abundant stirring and evenly mixing, heap fermentation, the steam sterilization sterilization treatment is used in the pack back, makes the even selenium enriched oyster mushroom of final composts or fertilisers of cultivating inoculated and cultured.
7. method according to claim 1 is characterized in that the maize cob meal that described selenium-enriching cultivation feed additives uses forms as the pulverizing of natural crops offcuts corncob.
8. method according to claim 1, the particle that it is characterized in that described selenium-enriching cultivation feed additives is by finishing granulation process after maize cob meal and the selenium yeast raw material pulverizing.
9. method according to claim 1, when it is characterized in that being used to keep healthy the cultivation of medicine food selenium enriched oyster mushroom, the Se content of the selenium-enriching cultivation feed additives of use is 100～200mg/kg; When being used for the cultivation of daily edible selenium enriched oyster mushroom, the Se content of the selenium-enriching cultivation feed additives of use is 5～50mg/kg.
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| CN102515884A (en) * | 2011-12-07 | 2012-06-27 | 苏州硒谷科技有限公司 | Edible fungi organic selenium additive, its preparation method and application |
| CN102630480A (en) * | 2012-04-01 | 2012-08-15 | 苏州安德鲁尹生物技术咨询有限公司 | Iron-rich black fungus and production method thereof |
| CN103039268A (en) * | 2012-12-11 | 2013-04-17 | 镇江市丹徒区南山溪园茶叶专业合作社 | Method for producing selenium-rich and zinc-rich Pleurotus geesteranus |
| CN103524173A (en) * | 2013-10-08 | 2014-01-22 | 开原市御隆粮米加工厂 | Agricultural tablet for improving rice nutrition |
| CN104521550A (en) * | 2014-12-01 | 2015-04-22 | 云南金雨庄生物科技有限责任公司 | Selenium-rich grifola frondosa cultivation method |
| CN107371786A (en) * | 2017-06-15 | 2017-11-24 | 柳城新天地生态农业发展有限公司 | The method for improving Se content in elegant precious mushroom |
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| CN102630480A (en) * | 2012-04-01 | 2012-08-15 | 苏州安德鲁尹生物技术咨询有限公司 | Iron-rich black fungus and production method thereof |
| CN103039268A (en) * | 2012-12-11 | 2013-04-17 | 镇江市丹徒区南山溪园茶叶专业合作社 | Method for producing selenium-rich and zinc-rich Pleurotus geesteranus |
| CN103524173A (en) * | 2013-10-08 | 2014-01-22 | 开原市御隆粮米加工厂 | Agricultural tablet for improving rice nutrition |
| CN104521550A (en) * | 2014-12-01 | 2015-04-22 | 云南金雨庄生物科技有限责任公司 | Selenium-rich grifola frondosa cultivation method |
| CN107371786A (en) * | 2017-06-15 | 2017-11-24 | 柳城新天地生态农业发展有限公司 | The method for improving Se content in elegant precious mushroom |
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Application publication date: 20110914 Assignee: Anhui Setek Biological Technology Co., Ltd. Assignor: Suzhou Se Technology Co., Ltd. Contract record no.: 2013320010174 Denomination of invention: Method for carrying out uniform selenium-enriched cultivation of pleurotus geesteranus by using selenium yeast Granted publication date: 20130403 License type: Exclusive License Record date: 20131224 |
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