CN102771313A - Method for culturing selenium-enriched agarics by utilizing selenium-enriched crop straws - Google Patents

Method for culturing selenium-enriched agarics by utilizing selenium-enriched crop straws Download PDF

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Publication number
CN102771313A
CN102771313A CN2012102901037A CN201210290103A CN102771313A CN 102771313 A CN102771313 A CN 102771313A CN 2012102901037 A CN2012102901037 A CN 2012102901037A CN 201210290103 A CN201210290103 A CN 201210290103A CN 102771313 A CN102771313 A CN 102771313A
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selenium
rich
particle
cultivation
auricularia auriculajudae
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罗磊
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SUZHOU SE TECHNOLOGY Co Ltd
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SUZHOU SE TECHNOLOGY Co Ltd
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Abstract

The invention provides a method for culturing selenium-enriched agarics by utilizing selenium-enriched crop straws. The method is realized by preparing culture materials by using basic culture materials and a selenium-enriched culture material additive. The method is characterized in that the selenium-enriched culture material additive accounts for 1-10% of the total weight of dry culture materials; the selenium content is 1-20mg/kg; the sizes of grains in the selenium-enriched culture material additive are in multi-size regional distribution in the range from 1.0mm to 5.0mm; and the selenium-enriched culture material additive is prepared from the straws of such crops as selenium-enriched wheat or corn with selenium content being 1-80mg/kg by grinding. The method has the following advantages: the agarics cultured by the method have high selenium absorption and utilization efficiency; the highest content of selenium in the fruiting bodies of the agarics can be 20 times that of selenium in the fruiting bodies of common culture groups; the organic selenium is as high as 90% of the total selenium; and in the growth periods of the fruiting bodies, the relative standard deviation (RSD) of the selenium contents of the fruiting bodies can be strictly controlled within 20%.

Description

Utilize rich selenium crops straw to cultivate the breeding method of rich selenium auricularia auriculajudae
Technical field
The present invention relates to edible mushroom, the breeding method of especially a kind of rich selenium auricularia auriculajudae belongs to the conversion technology field of selenium resource.
Background technology
Selenium is the trace element of needed by human; Be the essential composition of paddy light ammonia peptide peroxidase in vivo; Selenium has oxidation resistant effect; Closely bound up with health, that selenium has is anticancer, protection heart, treating liver necrosis, control myopia and cataract, detoxifcation, raising immunity, delay senility and strengthen multiple pharmacological effect such as reproductive function.According to incompletely statistics, have more than 40 countries and regions to lack selenium in the world, and China there is 72% area to belong to lack the selenium area, therefore, seek and develop rich selenium product, take in sufficient selenium to guarantee human body, just paid close attention to by the various countries scientific worker.
Auricularia auriculajudae is under the jurisdiction of Eumycota, Basidiomycetes, Agaricales, Auriculariaceae, Auricularia, edible, and quality is crisp, and China extensively cultivates at present.Wherein Uricularia polytricha is 90% to the inhibiting rate of small white mouse sarcoma 180, is 80% to the inhibiting rate of ehrlich carcinoma, contains in its fine hair and enriches polysaccharide, and anti-cancer and kill cancer action is arranged.Auricularia auriculajudae has higher drug value, and it is strong that it has enriching yin, clearing lung-heat benefit gas; Enrich blood and invigorate blood circulation; Functions such as hemostasis and pain-relieving are weaving and the good health food of mine worker, report according to the data of Japan again; Containing abundant polysaccharide in the fine hair at the Uricularia polytricha back side, is one of six kinds of the strongest medicinal fungus of antitumor activity (other are glossy ganoderma, rainbow conk, birch pleat pore fungi, artist's conk, red bolt bacterium).Many in recent years scholars think that cellulose is to keep the necessary nutrient of health, and coarse-fibred content is also higher, and this cellulose has also played good effect to digestion, absorption and the metabolism of many nutriments in the human body.
Auricularia auriculajudae has good selenium rich ability; What introduce in " cultivation method of black fungus rich in selenium " (number of patent application 201010154424.5), " producing culturing edible fungi such as rich selenium auricularia auriculajudae with selenium mineral powder " (number of patent application 200910067120.2), " containing the woodear production method of enriching organic selenium " technical schemes such as (number of patent application 201110099832.X) is the selenium-enriching cultivation method of woodear; Said method all is to sneak into sodium selenite solution in the employing medium to form the rich selenium auricularia auriculajudae of the medium culture that necessarily contains selenium concentration; But adopt auricularia auriculajudae that this method cultivates its to selenium to absorb efficient not high; The Se content that fruit body forms is not high yet, and the shared ratio of organic selenium is not high yet in the selenium form.The woodear mycelia production method of selenium " abundant contain " (number of patent application 201010555877.9) then is that what to introduce is to adopt the form of liquid fermentation to cultivate Auricularia mycelium; But this method can only be cultivated and obtained Auricularia mycelium; The time period is cultivated in its growth, and the Auricularia mycelium selenium form major part that obtains is an inorganic selenium.
Summary of the invention
The objective of the invention is: provide a kind of and utilize rich selenium crops straw to carry out the breeding method of rich selenium auricularia auriculajudae; Cultivate the auricularia auriculajudae that obtains with this method selenium absorbed the efficient height; Its fruit body Se content reaches as high as 20 times of common cultivation group fruit body Se content, and in the growth cycle of fruit body, the relative standard deviation RSD of fruit body selenium content can be strict controlled in 20%; The stability of the Se content of the high selenium auricularia auriculajudae that can guarantee to cultivate; The ratio of the shared total selenium of organic selenium is up to more than 90% in the auricularia auriculajudae fruit body in addition, and selenium form safety can be used as desirable selenium-enriched food and supplies human body edible.
Above-mentioned goal of the invention realizes through following technical scheme, is included in and sneaks into the selenium-enriching cultivation feed additives that accounts for cultivation siccative gross weight 1~10% in the cultivation siccative, cultivates according to the common cultivation method and obtains rich selenium auricularia auriculajudae; Wherein to contain the selenium total amount be 1~20 mg/kg to the selenium-enriching cultivation feed additives; And being many particle size region in the selenium-enriching cultivation feed additives in the scope of grain diameter below 5.0 mm distributes; Particle diameter is that the particle of 1.0~5.0 mm accounts for 10% of additive gross weight at least, and particle diameter is that the particle below 0.1 mm accounts for 50% of additive gross weight at least; Said selenium-enriching cultivation additive adopt the crushed stalk of the crops such as wheat or corn of rich selenium to form.
Preferably, be three particle size region in the said selenium-enriching cultivation feed additives in the scope of grain diameter below 5.0 mm and distribute, comprise with its weight percent meter in additive:
Particle diameter is the particle 10~20% of 1.0~5.0 mm;
Particle diameter is the particle 50~70% below 0.1 mm;
Particle diameter is the particle 10~30% of 0.1~1.0 mm.
Preferably, said cultivation siccative comprises with the weight percent meter of its component:
Cotton seed hulls 55%;
Wood chip 30%
Wheat bran 10%;
Quicklime 3%;
Land plaster 1%;
Composite fertilizer 1%.
Preferably; Agricultural crop straw powder such as Se-rich wheat that said selenium-enriching cultivation feed additives uses or corn are pulverized as agricultural crop straws such as the Se-rich wheat of artificial culture or selenium-rich corns and are formed, and the Se content of agricultural crop straw powder such as said Se-rich wheat or corn is 1~80 mg/kg.
Preferably; Said common cultivation method comprises the cultivation siccative of sneaking into the selenium-enriching cultivation feed additives according to material-water ratio 1:1.3 adding running water, abundant stirring and evenly mixing, heap fermentation; The steam sterilization sterilization treatment is used in the pack back, makes the rich selenium auricularia auriculajudae of final composts or fertilisers of cultivating inoculated and cultured.
Preferably, the particle of said selenium-enriching cultivation feed additives is accomplished through agricultural crop straws such as Se-rich wheat or corn being pulverized the back granulation process.
The concrete steps of the technical scheme that the present invention adopts can for:
At first prepare a kind of rich selenium fungus cultivation feed additives:
1. agricultural crop straws such as Se-rich wheat or corn being passed through screening, drying, are crushed to particle diameter is below 0.1 mm, and mixing is designed the required cultivate material additive of cultivation of selenium-rich auricularia auriculajudae, and selenium content is in 1~20 mg/kg scope.
2. the above-mentioned additive processing of part granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.1~1.0 mm, 1.0~5.0 mm scopes.
3. above-mentioned three kinds of particle diameters are respectively: below 0.1 mm, the particle of 0.1~1.0 mm, 1.0~5.0 mm mixes by following percentage by weight: 50~70%, 10~30%, 10~20%; Promptly obtain having the selenium-enriching cultivation feed additives of different grain size proportioning characteristic, its total Se content is still in 1~20 mg/kg scope.
Cultivation is during spice, and the ratio in 1~10% is sneaked into the common cultivation material with additive, stirs, and makes that final selenium content is in 0.1~2 mg/kg scope in the cultivate material.
Press material-water ratio 1:1.3 and add running water, fully stir, mixing once more, heap fermentation, handle with steam sterilizing the pack back, makes final cultivate material, the rich selenium auricularia auriculajudae of cultivation after inoculation.
When the auricularia auriculajudae fruit body is gathered, detect wherein Se content.The fruit body Se content has improved 2~20 times than common cultivation group, can reach 1~8 mg/kg, and the selenium that exists with organic selenium forms such as seleno-amino acids in the fruit body accounts for more than 90% of total selenium.In the growth cycle of fruit body, the relative standard deviation RSD of fruit body selenium content can be strict controlled in 20%, the stability of the Se content of the rich selenium auricularia auriculajudae that can guarantee to cultivate.
With respect to scheme of the prior art, the invention has the advantages that:
(1) the present invention has realized the rich selenium cultivation of auricularia auriculajudae fruit body; Utilize auricularia auriculajudae that the present invention cultivates that selenium in the medium absorbed the efficient height; Its fruit body selenium Se content can reach 1~8 mg/kg; Be 5~10 times of Se content in the cultivate material, the fruit body Se content is 2~20 times of common cultivation group fruit body Se content; And the ratio that organic selenium accounts for total selenium in the fruit body is more than 90%, and selenium form safety can be used as desirable selenium-enriched food and supplies human body edible;
(2) the cultivate material additive for preparing among the present invention; In conjunction with the characteristics of edible fungi growth, have different grain size proportions, can realize that wherein selenium discharges so that different rates is stable; Realize the stability of each damp massee fruiting bodies Se content, its relative standard deviation can be controlled in below 20%;
(3) additive component is simple and convenient, only needs during use when the cultivate material spice, to mix in proportion with the common cultivation material, promptly can be made into the cultivate material of selenium content in 0.1~2mg/kg scope.
Embodiment
Following examples are used to explain the present invention, but can not be used for limiting scope of the present invention.The implementation condition that adopts among the embodiment can be done further adjustment according to actual conditions, and unaccounted implementation condition is generally the condition in the normal experiment.
Embodiment 1: rich selenium fungus cultivation embodiment
The preparation of rich selenium auricularia auriculajudae additive
1. agricultural crop straws such as Se-rich wheat or corn being passed through screening, drying, are crushed to particle diameter is to design the required cultivate material additive of cultivation of selenium-rich auricularia auriculajudae below 0.1 mm, and its Se content is 5 mg/kg;
2. the above-mentioned compound processing of part granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.1~1.0 mm, 1.0~5.0 mm scopes;
3. above-mentioned three kinds of particle diameters are respectively: below 0.1 mm, the particle of 0.1~1.0 mm, 1.0~5.0 mm mixes by following percentage by weight: 50~70%, 10~30%, 10~20%; Promptly obtain having the selenium-enriching cultivation feed additives of different grain size proportioning characteristic, its total Se content is still in the 5mg/kg scope.
Auricularia auriculajudae is selenium nutrition-intensified:
Select the additive of above-mentioned preparation for use, during spice, the ratio in 10% is sneaked into the common cultivation material with additive, stirs in cultivation, makes that final selenium content is in 0.5 mg/kg scope in the cultivate material.
Press material-water ratio 1:1.3 and add running water, fully stir, mixing once more, heap fermentation, handle with steam sterilizing the pack back, makes the rich selenium auricularia auriculajudae of final cultivate material inoculated and cultured.
Implementation result:
When the auricularia auriculajudae fruit body was gathered, sample detecting is Se content wherein.The fruit body Se content has improved 2.5 times than common cultivation group, can reach 1 mg/kg, and Se content is 2 times of cultivate material selenium concentration, and the selenium that exists with organic selenium forms such as seleno-amino acids in the fruit body accounts for more than 90% of total selenium.In the growth cycle of fruit body, the relative standard deviation RSD of fruit body selenium content can be strict controlled in 20%, can realize the stability of fruit body Se content.
 
Embodiment 2: rich selenium fungus cultivation embodiment
The preparation of rich selenium auricularia auriculajudae additive
1. agricultural crop straws such as Se-rich wheat or corn being passed through screening, drying, are crushed to particle diameter is to design the required cultivate material additive of cultivation of selenium-rich auricularia auriculajudae below 0.1 mm, and its Se content is 10 mg/kg;
2. the above-mentioned compound processing of part granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.1~1.0 mm, 1.0~5.0 mm scopes;
3. above-mentioned three kinds of particle diameters are respectively: below 0.1 mm, the particle of 0.1~1.0 mm, 1.0~5.0 mm mixes by following percentage by weight: 50~70%, 10~30%, 10~20%; Promptly obtain having the selenium-enriching cultivation feed additives of different grain size proportioning characteristic, its total Se content is still in 10 mg/kg scopes.
Auricularia auriculajudae is selenium nutrition-intensified:
Select the additive of above-mentioned preparation for use, during spice, the ratio in 10% is sneaked into the common cultivation material with additive, stirs in cultivation, makes that final selenium content is in 1 mg/kg scope in the cultivate material.
Press material-water ratio 1:1.3 and add running water, fully stir, mixing once more, heap fermentation, handle with steam sterilizing the pack back, makes the rich selenium auricularia auriculajudae of final cultivate material inoculated and cultured.
Implementation result:
When the auricularia auriculajudae fruit body was gathered, sample detecting is Se content wherein.The fruit body Se content has improved 10 times than common cultivation group, can reach 4 mg/kg, and Se content is 4 times of cultivate material selenium concentration, and the selenium that exists with organic selenium forms such as seleno-amino acids in the fruit body accounts for more than 90% of total selenium.In the growth cycle of fruit body, the relative standard deviation RSD of fruit body selenium content can be strict controlled in 20%, can realize the stability of fruit body Se content.
 
Embodiment 3: rich selenium fungus cultivation embodiment
The preparation of rich selenium auricularia auriculajudae additive
1. agricultural crop straws such as Se-rich wheat or corn being passed through screening, drying, are crushed to particle diameter is to design the required cultivate material additive of cultivation of selenium-rich auricularia auriculajudae below 0.1 mm, and its Se content is 20 mg/kg;
2. the above-mentioned compound processing of part granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.1~1.0 mm, 1.0~5.0 mm scopes;
3. above-mentioned three kinds of particle diameters are respectively: below 0.1 mm, the particle of 0.1~1.0 mm, 1.0~5.0 mm mixes by following percentage by weight: 50~70%, 10~30%, 10~20%; Promptly obtain having the selenium-enriching cultivation feed additives of different grain size proportioning characteristic, its total Se content is still in the 20mg/kg scope.
Auricularia auriculajudae is selenium nutrition-intensified:
Select the additive of above-mentioned preparation for use, during spice, the ratio in 10% is sneaked into the common cultivation material with additive, stirs in cultivation, makes that final selenium content is in 2 mg/kg scopes in the cultivate material.
Press material-water ratio 1:1.3 and add running water, fully stir, mixing once more, heap fermentation, handle with steam sterilizing the pack back, makes the rich selenium auricularia auriculajudae of final cultivate material inoculated and cultured.
Implementation result:
When the auricularia auriculajudae fruit body was gathered, sample detecting is Se content wherein.The fruit body Se content has improved 20 times than common cultivation group, can reach 8 mg/kg, and Se content is 4 times of cultivate material selenium concentration, and the selenium that exists with organic selenium forms such as seleno-amino acids in the fruit body accounts for more than 90% of total selenium.In the growth cycle of fruit body, the relative standard deviation RSD of fruit body selenium content can be strict controlled in 20%, can realize the stability of fruit body Se content.

Claims (7)

1. one kind is utilized rich selenium crops straw to cultivate the breeding method of rich selenium auricularia auriculajudae; Through realizing by basic cultivate material and selenium-enriching cultivation feed additives configuration cultivate material; It is characterized in that: said selenium-enriching cultivation feed additives accounts for cultivation siccative gross weight 1~10%; Se content is 1~20 mg/kg, and grain diameter is many particle size region distributions in the selenium-enriching cultivation feed additives in 1.0~5.0 mm scopes.
2. the rich selenium crops straw of utilization according to claim 1 is cultivated the breeding method of rich selenium auricularia auriculajudae; It is characterized in that: the particle that said selenium-enriching cultivation feed additives particle diameter is 1.0~5.0 mm accounts for 10% of additive gross weight at least, and particle diameter is that the particle below 0.1 mm accounts for 50% of additive gross weight at least.
3. the rich selenium crops straw of utilization according to claim 1 is cultivated the breeding method of rich selenium auricularia auriculajudae, it is characterized in that: said selenium-enriching cultivation feed additives particle diameter is distributed as the particle that particle diameter is 1.0~5.0 mm, 10~20%; Particle diameter is the particle of 0.1~1.0 mm, 10~30%; Particle diameter is the particle 50~70% below 0.1 mm.
4. the rich selenium crops straw of utilization according to claim 1 is cultivated the breeding method of rich selenium auricularia auriculajudae, it is characterized in that: the crushed stalk of crops such as Se-rich wheat that said selenium-enriching cultivation feed additives employing Se content is 1~80 mg/kg or corn forms.
5. the rich selenium crops straw of utilization according to claim 1 is cultivated the breeding method of rich selenium auricularia auriculajudae, it is characterized in that: said cultivation siccative comprises with the weight percent meter of its component: with cotton seed hulls, and 55%; Wood chip, 30%; Wheat bran, 10%; Quicklime, 3%; Land plaster, 1%; Composite fertilizer, 1%.
6. the rich selenium crops straw of utilization according to claim 1 is cultivated the breeding method of rich selenium auricularia auriculajudae; It is characterized in that: comprise the cultivation siccative of sneaking into the selenium-enriching cultivation feed additives is added running water according to material-water ratio 1:1.3; Abundant stirring and evenly mixing; Heap fermentation, the steam sterilization sterilization treatment is used in the pack back, makes the rich selenium auricularia auriculajudae of final composts or fertilisers of cultivating inoculated and cultured.
7. the rich selenium crops straw of utilization according to claim 1 is cultivated the breeding method of rich selenium auricularia auriculajudae; It is characterized in that: comprise following step: 1. agricultural crop straws such as Se-rich wheat or corn being passed through screening, drying, are crushed to particle diameter is below 0.1 mm; Mixing; Design the required cultivate material additive of cultivation of selenium-rich auricularia auriculajudae, selenium content is in 1~20 mg/kg scope; 2. the above-mentioned additive processing of part granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.1~1.0 mm, 1.0~5.0 mm scopes; 3. above-mentioned three kinds of particle diameters are respectively: below 0.1 mm, the particle of 0.1~1.0 mm, 1.0~5.0 mm mixes by following percentage by weight: 50~70%, 10~30%, 10~20%; When 4. cultivating spice, the ratio in 1~10% is sneaked into the common cultivation material with additive, stirs, and makes that final selenium content is in 0.1~2 mg/kg scope in the cultivate material; Press material-water ratio 1:1.3 and add running water, fully stir, mixing once more, heap fermentation, handle with steam sterilizing the pack back, makes final cultivate material, the rich selenium auricularia auriculajudae of cultivation after inoculation.
CN2012102901037A 2012-08-15 2012-08-15 Method for culturing selenium-enriched agarics by utilizing selenium-enriched crop straws Pending CN102771313A (en)

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CN103951507A (en) * 2014-04-16 2014-07-30 伊春金瑞森林食品有限责任公司 Selenium containing agaric cultivating material
CN104402639A (en) * 2014-12-23 2015-03-11 河南牧业经济学院 Selenium-rich black fungus culture material and preparation method thereof
CN104402640A (en) * 2014-12-23 2015-03-11 河南牧业经济学院 Selenium-rich Flammulina velutipes culture material and preparation method thereof
CN104557201A (en) * 2014-12-29 2015-04-29 河南牧业经济学院 Selenium-rich white fungus cultivation material and preparation method thereof
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CN106961965A (en) * 2017-03-27 2017-07-21 福建华农生物科技有限公司 A kind of implantation methods of selenium-rich roxburgh anoectochilus terminal bud

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CN103183568A (en) * 2013-04-19 2013-07-03 吴庆旺 Organic selenium-rich fertilizer and preparation method thereof
CN103183568B (en) * 2013-04-19 2015-06-03 吴庆旺 Organic selenium-rich fertilizer and preparation method thereof
CN103951507A (en) * 2014-04-16 2014-07-30 伊春金瑞森林食品有限责任公司 Selenium containing agaric cultivating material
CN104402639A (en) * 2014-12-23 2015-03-11 河南牧业经济学院 Selenium-rich black fungus culture material and preparation method thereof
CN104402640A (en) * 2014-12-23 2015-03-11 河南牧业经济学院 Selenium-rich Flammulina velutipes culture material and preparation method thereof
CN104557201A (en) * 2014-12-29 2015-04-29 河南牧业经济学院 Selenium-rich white fungus cultivation material and preparation method thereof
CN104557304A (en) * 2014-12-29 2015-04-29 河南牧业经济学院 Selenium-rich shii-take cultivation material and preparation method thereof
CN104672008A (en) * 2015-02-05 2015-06-03 灵川县农业局 Selenium-enriched black fungus culture material and preparation method thereof
CN105016816A (en) * 2015-07-17 2015-11-04 安徽省农业科学院土壤肥料研究所 Selenium-rich black fungus cultivation base material
CN105272443A (en) * 2015-09-23 2016-01-27 百色市右江区永乐三宝农产品有限公司 Se-enriched Baise black fungus production medium additive and application method
CN105907645A (en) * 2016-04-27 2016-08-31 佛山市泓丞生物科技有限公司 Solid strain formula for biologically converting inorganic selenium into organic selenium and production technology
CN106258491A (en) * 2016-08-17 2017-01-04 王瑜琳 A kind of cultural method of selenium-rich Auricularia
CN106358753A (en) * 2016-08-26 2017-02-01 福州市凯达生态农业有限公司 Inoculation process for edible fungi
CN106819678A (en) * 2016-11-30 2017-06-13 苏州硒谷科技有限公司 Black fungus rich in selenium beverage and preparation method thereof
CN106961965A (en) * 2017-03-27 2017-07-21 福建华农生物科技有限公司 A kind of implantation methods of selenium-rich roxburgh anoectochilus terminal bud

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