CN102160551A - Transsexuality modifier for female flowers and male flowers of jatropha curcas and preparation method thereof - Google Patents
Transsexuality modifier for female flowers and male flowers of jatropha curcas and preparation method thereof Download PDFInfo
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- CN102160551A CN102160551A CN2011100542980A CN201110054298A CN102160551A CN 102160551 A CN102160551 A CN 102160551A CN 2011100542980 A CN2011100542980 A CN 2011100542980A CN 201110054298 A CN201110054298 A CN 201110054298A CN 102160551 A CN102160551 A CN 102160551A
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Abstract
The invention discloses a transsexuality modifier for female flowers and male flowers of jatropha curcas and a preparation method thereof. The modifier comprises a plant exogenous hormone dispensing agent, wherein the plant exogenous hormone dispensing agent comprises the following components in part by weight: 1 to 50 parts of S-abscisic acid, 1 to 20 parts of cyclodextrin, 1 to 20 parts of vincofos, 1 to 50 parts of cytokinin and 1 to 50 parts of D-Iso ascorbic acid. By the female and male flowers transsexuality modifier which is used for a jatropha curcas forest and has fruit setting capability, the number of the female flowers of the jatropha curcas can be increased by orientated induction, the ratio of the total number of flowers to the number of the female flowers is obviously increased, bisexual flowers can be induced, the total number of the induced jatropha curcas inflorescence is improved by over 200 percent, the ratio of the number of the female flowers to the total number of the flowers is improved by over 55 percent after the transsexuality modification, and the flowering and fruit setting capability of the jatropha curcas can be effectively improved, so that the fruit yield of the jatropha curcas can be improved to a great extent.
Description
Technical field
The present invention relates to chemical agent of a kind of trees floral organ ratio of regulating and control to grow and preparation method thereof, relating to a kind of little seeds of a tung oil tree male and female flowering particularly changes property conditioning agent and preparation method thereof.
Background technology
What the little seeds of a tung oil tree (Jatropha curcas) were that current international energy circle generally acknowledges also is first-selected renewable energy resources plant, and the oil plant that its seed benevolence is squeezed out is biodiesel through simple and easy processing, can be widely used in all kinds of dynamic power machines and diesel locomotive.3 tons of small idesias just can be produced 1 ton of biodiesel, are the biological new forms of energy kinds that has competitiveness and strategy exploitation meaning on the international market.
No matter (cloud, expensive, river, Guangdong and Guangxi Provinces and Hainan) is original natural forest or artificial planting woods in number provinces and regions, China southwest, and all with considerable scale, only PetroChina Company Limited. just reaches 1,000,000 mu in state, the Liangshan Mountain, Sichuan.But as the bioenergy raw material production forest of commercialization, scale, its per mu yield is too low, and every mu is no more than 100 kilograms, lacks improved seeds and high-yield culture technique measure in the production.The phenomenon of " industry is leading, and research and development lag behind " seriously restricts the sound development of China's bioenergy industry.As seen, improving the output of the little seeds of a tung oil tree on a large scale, is the industry bottleneck problem that needs to be resolved hurrily.The quality and the output that improve little seeds of a tung oil tree woods rapidly will be one of crucial directions of recent little seeds of a tung oil tree industry research.
At present, the too low one of the main reasons of little seeds of a tung oil tree woods per mu yield is to there is no the good clone new varieties of high yield in the little seeds of a tung oil tree production can promote.On the other hand, the little seeds of a tung oil tree of having planted always are the breedings of seed seedling, the existing little seeds of a tung oil tree belong to seedling crop entirely, have the serious balance phenomenon of male and female flowering ratio after blooming, and the little seeds of a tung oil tree are the different flowering plants of monoecism, the quantity of female flower is few, account for greatly and always spend severally 1.5%～5%, the male flower ratio is too high, does not have enough female flowers, fruit-setting rate is low, has a strong impact on little seeds of a tung oil tree fruit yield.In the long term, must cultivate the degeneration-resistant improved seeds of the high oil of high yield.But to the present little seeds of a tung oil tree of seedling crop of implant mass, at the serious unbalance too low phenomenon of fruit yield that causes of back male and female flowering ratio of blooming, working out to take effect in a short time improves the method for little seeds of a tung oil tree output, just seem particularly important, particularly bedding-in goes out a kind of chemicals, little seeds of a tung oil tree floral organ device can be changed than raising female flower ratio by using this chemicals, thereby little seeds of a tung oil tree fruit yield can be improved.
Summary of the invention
In order to solve the problem that above-mentioned prior art exists, the invention provides a kind of little seeds of a tung oil tree male and female flowering changes the property adjustment, thereby changes property adjustment and can improve the female flower ratio and improve little seeds of a tung oil tree fruit yield by using this little seeds of a tung oil tree male and female flowering.The present invention also provides the preparation method of this little seeds of a tung oil tree male and female flowering commentaries on classics property adjustment.
The technical solution used in the present invention is: a kind of little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent, it comprises plant exogenous hormone ingredients, and described plant exogenous hormone ingredients further comprises following components by weight portion: 1～20 part cyclodextrin, 1～20 part ethephon, S-abscisic acid of 1～50 part, 1～50 part D-arabo-ascorbic acid, 1～50 part cytokinin.
Preferably, described plant exogenous hormone ingredients comprises following components by weight portion: 5～15 parts cyclodextrin, 6～18 parts ethephon, S-abscisic acid of 15～35 parts, 12～35 parts D-arabo-ascorbic acid, 22～35 parts cytokinin.
Preferred, described plant exogenous hormone ingredients comprises following components by weight portion: 6 parts cyclodextrin, 15 parts ethephon, S-abscisic acid of 35 parts, 18 parts D-arabo-ascorbic acid, 26 parts 6-cytokinin.
Described cyclodextrin is preferred-cyclodextrin or its derivative, more preferably hydroxypropyl--cyclodextrin.
Described cytokinin can be one or more in zeatin, isopentennyladenine, ribosylzeatin, isopentenyladenosine, N6-furans first (base) adenine, the 6-benzyl aminoadenine (6-BA), is preferably 6-benzyl aminoadenine.
Preferably, the above little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent also includes the glacial acetic acid solvent, and described plant exogenous hormone ingredients is dissolved in and forms solution in the glacial acetic acid.More preferably, it is that male and female flowering changes property conditioning agent stoste that described little seeds of a tung oil tree male and female flowering changes the property conditioning agent, described male and female flowering change that property conditioning agent stoste adds by the glacial acetic acid solution of described plant exogenous hormone ingredients that water is made into and wherein the concentration of plant exogenous hormone ingredients be 50mg/L～2000mg/L.Better, the concentration of plant exogenous hormone ingredients is 100mg/L～1000mg/L in the described male and female flowering commentaries on classics property conditioning agent stoste.
The preparation method of little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent of the present invention, may further comprise the steps: the plant exogenous hormone ingredients of above-mentioned any one composition is dissolved in glacial acetic acid solution adds water then and be made into, and wherein the concentration of plant exogenous hormone ingredients is 50mg/L～2000mg/L.
Technique effect of the present invention is:
1, uses male and female flowering of the present invention commentaries on classics property conditioning agent the little seeds of a tung oil tree woods that possesses the ability of bearing fruit, orientable instaminate flower formation of inducing the little seeds of a tung oil tree of raising, significantly the ratio of number and female flower is always spent in increase, simultaneously can induce hermaphrodite flower, the number of always spending of the little seeds of a tung oil tree inflorescence after inducing has improved more than 200%, female flower number after the regulation and control of commentaries on classics property accounts for always spends several ratios to improve more than 55%, can effectively promote the one-tenth flower of the little seeds of a tung oil tree to bear fruit, thereby increase substantially quantity as a result, the seed production of individual plant can be improved 2～4 times.
2, can transform the low yield forest land under the prerequisite of not damaging the scale running of original little seeds of a tung oil tree forest land, shorten fruit-growing time, volume increase rapidly improves the forest land benefit.
Embodiment
Can further be well understood to the present invention by specific embodiments of the invention given below.But they are not limitation of the invention.
Embodiment 1
Take by weighing each component of plant exogenous hormone ingredients by following weight proportion and place container: 5g-cyclodextrin, the ethephon of 18g, S-abscisic acid of 30g, the D-arabo-ascorbic acid of 12g, the 6-benzyl aminoadenine of 35g, the plant exogenous hormone ingredients that weighs up is dissolved in the glacial acetic acid solution that glacial acetic acid makes plant exogenous hormone ingredients, and the consumption of glacial acetic acid just is dissolved as suitable with plant exogenous hormone ingredients fully.And then adding distilled water, be made into the little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent stoste that contains plant exogenous hormone ingredients 100mg in every liter of solution.
Embodiment 2
Take by weighing each component of plant exogenous hormone ingredients by following weight proportion and place container: 6g-cyclodextrin, the ethephon of 15g, S-abscisic acid of 35g, the D-arabo-ascorbic acid of 18g, N6-furans first (base) adenine of 26g, the plant exogenous hormone ingredients that weighs up is dissolved in the glacial acetic acid solution that glacial acetic acid makes plant exogenous hormone ingredients, and the consumption of glacial acetic acid just is dissolved as suitable with plant exogenous hormone ingredients fully.And then adding distilled water, be made into the little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent stoste that contains plant exogenous hormone ingredients 600mg in every liter of solution.
Embodiment 3
Take by weighing each component of plant exogenous hormone ingredients by following weight proportion and place container: the hydroxypropyl of 13g--cyclodextrin, the ethephon of 15g, S-abscisic acid of 15g, the D-arabo-ascorbic acid of 35g, the 6-benzyl aminoadenine of 22g, adding glacial acetic acid and stirring make plant exogenous hormone ingredients dissolve the formation glacial acetic acid solution fully, and the consumption of glacial acetic acid just is dissolved as suitable with plant exogenous hormone ingredients fully.And then adding distilled water, be made into the little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent stoste that contains plant exogenous hormone ingredients 1000mg in every liter of solution.
Embodiment 4
Take by weighing each component of plant exogenous hormone ingredients by following weight proportion and place container: the hydroxypropyl of 20g--cyclodextrin, the ethephon of 18g, S-abscisic acid of 3g, the D-arabo-ascorbic acid of 50g, the 6-benzyl aminoadenine of 9g, adding glacial acetic acid and stirring makes plant exogenous hormone ingredients dissolve the formation glacial acetic acid solution fully, and then adding distilled water, be made into the little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent stoste that contains plant exogenous hormone ingredients 720mg in every liter of solution.
Embodiment 5
The application effect example, adopt following method that the little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent of embodiment 1～4 preparation is applied to little seeds of a tung oil tree woods:
Step 1: the selection of plant, processing and little seeds of a tung oil tree male and female flowering change using of property conditioning agent
Select to survive after the field planting and possess flower bear fruit ability, the Jatropha curcas plant of the age of tree more than 2 years, before bud occurs the 25th day to the bud appearance the 5th day during in, selected little seeds of a tung oil tree butt is in the one side 25cm of the moon scope, with radius is that the addle Lao Pi at position of the no scar of 5cm wipes off and exposes new skin, use 70% alcohol disinfecting immediately, 500 times of water diluents with bordeaux mixture spray sterilization again.Then, suppressing syringe or trees high-pressure injection machine with trees changes property conditioning agent stoste with little seeds of a tung oil tree male and female flowering and injects xylem through debridement, sterilization, sterilization treatment position, or use the screw extrusion form, make injection needle screw in treated position, in trunk, throw little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent stoste into by injection needle again.Consumption is controlled to be 35～45 milliliters/strain.Injection is finished immediately and is embrocated 70% alcohol disinfecting to extracting the syringe needle position, and sprays sterilization with 500 times of water diluents of bordeaux mixture.
Step 2: use the microbial bacteria liquid irrigating root
The Jatropha curcas plant that step 1 has been used little seeds of a tung oil tree male and female flowering and change the property conditioning agent is strengthened the supply of moisture, fertilizer and is managed and protected, carry out intertill and clean tillage, occur at the plant bud the 1st day occur in later the 10th day, 100 times of dilute aqueous solutions filling roots of each Jatropha curcas plant with multiple-effect microbial bacterium EM stoste (liking that peaceful your environment friendly biological technology (Nanjing) Co., Ltd produces) to bud.
Step 3: use little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent once more
In the same period of step 2, after selecting the base portion of trunk or branch to carry out debridement, sterilization, sterilization treatment according to the method for step 1 through each Jatropha curcas plant of filling root, with suppress syringe or trees high-pressure injection machine with trees 300 times of water dilute liquid medicines of male and female flowering conditioning agent stoste are injected the xylem at treated position, amount of application is 40～60 milliliters/strain.The purpose in this step is that directed regulation and control flower bud differentiation develops to the direction that forms more hermaphrodite flower and instaminate flower, further the male and female flowering ratio after the regulation and control of raising commentaries on classics property.
Step 4: use to protect and spend the agent of strong fruit
When the Jatropha curcas plant of using the male and female flowering conditioning agent through step 3 is once more bloomed; (have instaminate flower, male flower and teleianthous one period simultaneously) in the florescence peak period to each Jatropha curcas plant and spray to protect and spend the agent of strong fruit; can adopt commercially available various guarantors to spend the agent of strong fruit, the tetramethyl glutaric acid aqueous solution that also can prepare 10mg/L～500mg/L voluntarily sprays.
The little seeds of a tung oil tree of multiple low yield are used each 20,000 strain of male and female flowering commentaries on classics property conditioning agent stoste of the embodiment of the invention 1,2,3,4 respectively; be respectively experimental group 1, experimental group 2, experimental group 3, experimental group 4; with the microbial bacteria liquid irrigating root and use to protect and spend the strong really little seeds of a tung oil tree of same strain number of agent to organize in contrast to compare, its result is as follows with only:
always spend number, female flower that experimental group 1 is produced account for and always spend several ratios, fruit quantity and seed production to improve 2.6 times, 57%, 3.4 times and 2.8 times respectively than control group;
always spend number, male and female flowering ratio, fruit quantity and seed production that experimental group 2 is produced have improved 2.5 times, 57%, 3.3 times and 2.7 times respectively than control group;
always spend number, male and female flowering ratio, fruit quantity and seed production that experimental group 3 is produced have improved 2.8 times, 59%, 3.5 times and 2.9 times respectively than control group;
always spend number, male and female flowering ratio, fruit quantity and seed production that experimental group 4 is produced have improved 2.8 times, 60%, 3.6 times and 3.0 times respectively than control group;
Use male and female flowering of the present invention commentaries on classics property conditioning agent, experimental group individual plant volume increase 5～10kg, the seed oil content also brings up to 34.8%～36.7% by 31.7% of control group.
Claims (10)
1. one kind little seeds of a tung oil tree male and female flowering changes the property conditioning agent, it is characterized in that: described little seeds of a tung oil tree male and female flowering changes the property conditioning agent and comprises plant exogenous hormone ingredients, and described plant exogenous hormone ingredients comprises following components by weight portion:
1～20 part of cyclodextrin,
1～20 part of ethephon,
1～50 part of S-abscisic acid,
1～50 part of D-arabo-ascorbic acid,
1～50 part of cytokinin.
2. little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent according to claim 1, it is characterized in that: described plant exogenous hormone ingredients comprises following components by weight portion:
5～15 parts of cyclodextrin,
6～18 parts of ethephons,
15～35 parts of S-abscisic acids,
12～35 parts of D-arabo-ascorbic acids,
22～35 parts of cytokinins.
3. little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent according to claim 2, it is characterized in that: described plant exogenous hormone ingredients comprises following components by weight portion:
6 parts of cyclodextrin,
15 parts of ethephons,
35 parts of S-abscisic acids,
18 parts of D-arabo-ascorbic acids,
26 parts of cytokinins.
4. according to claim 1,2 or 3 described little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agents, it is characterized in that: described cyclodextrin is-cyclodextrin or its derivative.
5. change property conditioning agent according to each described little seeds of a tung oil tree male and female flowering of claim 1 to 4, it is characterized in that: described cyclodextrin be hydroxypropyl--cyclodextrin.
6. according to each described little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent of claim 1 to 5, it is characterized in that: described cytokinin is a 6-benzyl aminoadenine.
7. according to each described little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent of claim 1 to 6, it is characterized in that: described little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent also includes the glacial acetic acid solvent, and described plant exogenous hormone ingredients is dissolved in and forms solution in the glacial acetic acid.
8. little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent according to claim 7, it is characterized in that: it is that male and female flowering changes property conditioning agent stoste that described little seeds of a tung oil tree male and female flowering changes the property conditioning agent, described male and female flowering change that property conditioning agent stoste adds by the glacial acetic acid solution of described plant exogenous hormone ingredients that water is made into and wherein the concentration of plant exogenous hormone ingredients be 50mg/L～2000mg/L.
9. little seeds of a tung oil tree male and female flowering commentaries on classics property conditioning agent according to claim 8 is characterized in that: the concentration of plant exogenous hormone ingredients is 100mg/L～1000mg/L in the described male and female flowering commentaries on classics property conditioning agent stoste.
10. preparation method who changes the property conditioning agent as each described little seeds of a tung oil tree male and female flowering in the claim 1～8, it is characterized in that, may further comprise the steps: described plant exogenous hormone ingredients is dissolved in glacial acetic acid adds water then and be made into, and wherein the concentration of plant exogenous hormone ingredients is 50mg/L～2000mg/L.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110054298 CN102160551B (en) | 2011-03-08 | 2011-03-08 | Transsexuality modifier for female flowers and male flowers of jatropha curcas and preparation method thereof |
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| CN 201110054298 CN102160551B (en) | 2011-03-08 | 2011-03-08 | Transsexuality modifier for female flowers and male flowers of jatropha curcas and preparation method thereof |
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| CN102160551B CN102160551B (en) | 2013-07-31 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104170623A (en) * | 2014-08-08 | 2014-12-03 | 深圳市新华南方生物科技股份有限公司 | Method capable of enabling jatropha curcas to blossom and bear fruit concentratedly |
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2011
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Patent Citations (5)
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| CN1943348A (en) * | 2005-10-08 | 2007-04-11 | 中国科学院成都生物研究所 | Plant growth regulating composition with inhibiting plant tip growth function |
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| WO2010116259A2 (en) * | 2009-04-07 | 2010-10-14 | Taminco, Naamloze Vennootschap | Plant growth regulator additive |
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| CN104170623A (en) * | 2014-08-08 | 2014-12-03 | 深圳市新华南方生物科技股份有限公司 | Method capable of enabling jatropha curcas to blossom and bear fruit concentratedly |
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