CN102144572A - Closed rotary maze device for animal memory training system - Google Patents

Closed rotary maze device for animal memory training system Download PDF

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Publication number
CN102144572A
CN102144572A CN2011100526371A CN201110052637A CN102144572A CN 102144572 A CN102144572 A CN 102144572A CN 2011100526371 A CN2011100526371 A CN 2011100526371A CN 201110052637 A CN201110052637 A CN 201110052637A CN 102144572 A CN102144572 A CN 102144572A
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maze
animal
rotating disk
motor
training system
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CN102144572B (en
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朱启文
赵建文
匡宝平
曹伟平
商丽宏
王克威
李玉生
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Shenyang Medical College
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Shenyang Medical College
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Priority to CN201110052637.1A priority Critical patent/CN102144572B/en
Priority to PCT/CN2011/071865 priority patent/WO2012119322A1/en
Priority to GB1309005.5A priority patent/GB2498685B/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K1/00Housing animals; Equipment therefor
    • A01K1/02Pigsties; Dog-kennels; Rabbit-hutches or the like
    • A01K1/03Housing for domestic or laboratory animals
    • A01K1/031Cages for laboratory animals; Cages for measuring metabolism of animals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K15/00Devices for taming animals, e.g. nose-rings or hobbles; Devices for overturning animals in general; Training or exercising equipment; Covering boxes
    • A01K15/02Training or exercising equipment, e.g. mazes or labyrinths for animals ; Electric shock devices ; Toys specially adapted for animals

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Health & Medical Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Clinical Laboratory Science (AREA)
  • Catching Or Destruction (AREA)
  • Housing For Livestock And Birds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Electrically Operated Instructional Devices (AREA)

Abstract

The invention discloses a closed rotary maze device for an animal memory training system. The maze device comprises a round box body and a circuit control system. Key technical points of the maze device are that: the bottom of the box body of the maze device is provided with a turntable driven by a motor; the motor is arranged on a central cylinder, and an output shaft of the motor is in fastened with the turntable along the axial direction of the central cylinder; the turntable rotates around the central cylinder under the driving of the motor; the periphery of the lower part of a frame of the box body is provided with a plurality of pulleys for supporting the turntable; and a sieve-shaped supporting net is arranged above the turntable. The maze device overcomes the defect of the lateralization in the detection of the conventional maze devices such as a Y-maze and a T-maze. In addition, a device driven by gradient voltage is removed; the maze device effectively prevents animals from finishing experiments by means of smell, and respective disadvantages of a Morris water maze, the Y-maze and a drought maze are weighted; the starlight detection can be performed through program control according to the nocturnal habits of the experimental animals; and the maze device can ensure that the experimental animals are subjected to sensory stimulation in real environment, which is more favorable for acquisition of experimental data.

Description

The closed rotary labyrinth device that is used for animal memory training system
Technical field
The present invention relates to a kind of about studying the model of setting up animal learning and memory behavior training method, particularly a kind of closed rotary labyrinth device that is used for animal memory training system.Can be applicable to fields such as neuro-physiology, Neuropharmacology and psychology.
Background technology
At present, the animal behavior experiment is the indispensable important means of research higher brain function and other Neuscience, especially has position more importantly aspect the behavioral medicine researchs such as neuro-physiology, Neuropharmacology.Wherein maze experiment is the classical project of behavioral experiment, is one of the most frequently used experiment of research rat (or mouse) learning and memory function, also is cognitive drug screening animal model commonly used.
In the task of labyrinth, the instrument of labyrinth device itself can inform that animal needs them how to do (instruction that is similar to human experimentation).In this task, the researcher generally can mention working memory and reference memory (Reference Memory) these two notions.Animal will be finished a subtask, what at first need is with reference to memory, it has reflected that animal is to the study of relevant information between each examination time (Trial) in the task (such as the appearance in spatial cues position, labyrinth and experimental rules etc.), therefore animal should be present in each experimentation with reference to information in the process that forms with reference to memory.In the situation of laboratory, the working memory of animal is meant the ability that keeps information between the examination different in every wheel the (Session) experiment time.Comparatively speaking, working memory is different between each examination is inferior, is the memory capability that animal keeps each examination time information (place of just being).After an examination was inferior, rat can wipe current specific working memory, but kept constant with reference to memory.Therefore working memory only works in an examination is inferior, then belongs to long-term memory with reference to memory.
Existing labyrinth device mainly contains following several: 1, animal working memory model is measured in non-irrigated labyrinth: non-irrigated labyrinth mainly is meant various radiation traps (radial arm maze), Y labyrinth, T labyrinth etc.Y, T type labyrinth and Barnes maze experiment do not provide bonus penalty terms, be the nature of utilizing animal to explore fully, this class labyrinth task is learning and memory under the hungry motivation of animal drives, therefore test the food intake of wanting strict control animal in the last week, its physique amount is alleviated to original about 85%.But the problem that the method exists is exactly to influence experimental result being existed around non-study factors such as arm walking nature and hunger intensities of animal.
2, water maze is measured animal working memory model: classical water maze model is that (Morris water maze MWM), utilizes animal to avoid water logging and the escape motivation of seeking platform is carried out the learning and memory task to the Morris water maze.Adding entry in the experimental provision on the basis in non-irrigated labyrinth in addition in the past, forming 8 arm radiation water mazes or T water maze, Y water maze etc., to eliminate of the influence of smell clue the experiment achievement.When utilizing water maze to carry out performance testing, do not need to control animal diet followed drinking-water; Required time of animal training is shorter, about 1 week; Can utilize calculator to set up image and gather automatically and analytical system, this just can make corresponding histogram and running orbit figure according to the data of being gathered, and is convenient to the researcher experimental result is done further analysis and discussion etc.But the shortcoming of MWM is that latent period and its motion function state of animal swimming are closely related, so influence the normal result that the state of the medicine of animal movement function and animal itself all might influence experiment.In addition, owing to need surveillance and analysis software, have only minority to equip preferably the laboratory and can carry out the work.The factor that the design of experimental arrangement need be considered is more, need the experimenter to possess the knowledge of certain neuro-physiology, cognitive physiology and mathematical statistics aspect simultaneously, bring difficulty for the explanation of carrying out with the result of experiment, also limited deeply using widely of this labyrinth simultaneously; Because physical demands is too big, body temperature is also lost too much, and old pindone becomes difficulty of task; Be not that all mouse strains all are suitable for the test of Morris labyrinth, (achievement does not improve with the increase of fate, and the school grade of 129/SvJ is deviation also can not to learn this task as BALB/c; Achievement difference between individuality is huge; Some strain, will encounter difficulties when finishing the learning and memory task based on vision when aging because the has age correlation is looked the road pathology as the mouse of 129/SvJ strain.In the C57BL/6 strain, because serious alopecia exists, it is depressed that some mouse is produced, and adds the ulcer dermatitis, finally may influence the swimming ability of mouse and influence the experiment achievement; To going down of slight ability of learning and memory, this labyrinth program may be insensitive; Animal immersed to cause internal secretion or other stress effect in the water.The interaction of the latter and cerebral lesion or pharmacology operation room has uncertainty; Still need just can finish through the operation of automatic monitor system by means of manual, this experiment is become some are dull; Shared experiment place is excessive.
3, other mouse behavior models: disposable passive avoidance and diving tower (or pole-climbing) model is the model of two kinds of better simply research learning memories, is applicable to that the research memory keeps and memory is consolidated.These two models all are how long the record animal does not enter to the cell of electric shock after suffering once to shock by electricity or do not jump off platform and test the maintenance of memory and consolidation; New object identification experiment is a kind of model of measuring animal non-space working memory ability, has utilized the congenital principle that new object is had the tendency explored of animal.In the testing period, if animal has been remembered the past heritage body, then animal will be less than new object to the exploration time of past heritage body.
To sum up, the labyrinth device of above-mentioned classics is the limitation of various degrees still, and human factor is more, and the technology operation is loaded down with trivial details, lacks the combined influence factor of conditional stimulus such as electricity, sound (noise) and light etc.For addressing the above problem, the applicant is through constantly research, the technical scheme of number of patent application for " CN200610045868.9 " disclosed: the sense of hearing of a kind of mouse, vision memory model and intelligent behavior detection system, the sense of hearing of this mouse, vision memory model comprises and has data collection zone, the labyrinth of purpose district and interface channel thereof, the circular camera bellows formula labyrinth of multichannel closed-loop path is adopted in this labyrinth, in the casing at the bottom of it has loam cake and fence, by dividing plate cabinets cavity is separated at least three test sections, one of them is the purpose district, the remainder data acquisition zone, each dividing plate bottom is the identical hole shape passage that has adjustable unilateral gate of distribution outward appearance equidistantly, each hole shape access door side has two groups of infrared emission, receive photoelectric cell, in segregate each district the controllable lamp optical illumination is set respectively, the electric shock conductor of the different gradient voltage bands of generation of STOCHASTIC CONTROL is set respectively at the bottom of the fence in each district, in the control cabinet of casing middle standing pillar top, the high light generating means is set, noise generating means and video tracking head.This sense of hearing, vision memory model utilize the controllable lamp optical illumination to adapt to the dark habit that becomes of mouse, make it in casing, carry out autokinetic movement, different gradient voltage bands in conjunction with the electric shock conductor produces stimulate the passive movement that drives the STOCHASTIC CONTROL mouse with electric shock, and fasting and non-fasting mouse all are suitable for.Force the training of animal swimming different with existing water maze, adopt the autokinetic movement and the passive driving of gradient voltage of mouse nature activity pattern to combine, preventing to be tried mouse accepts after the single electro photoluminescence away from dedicated tunnel, it is inconsistent to avoid animal pattern respectively to distinguish the time of staying at data acquisition unit, makes time class data more accurate.The infrared emission of each passage reveal of labyrinth, reception photoelectric cell can detect the whereabouts of animal in the labyrinth in real time, exactly.Yet the applicant finds this labyrinth model and still has the following disadvantages: shocking by electricity to stimulate drives its effect gentleness inadequately, and the passive driving of gradient voltage is easily to laboratory animal generation infringement in various degree, and setting is comparatively complicated; In addition, install perfectly inadequately, laboratory animal may rely on smell finish experiment, and above-mentioned shortcoming all can influence experimental result, influences the accuracy of experimental data.
In sum, according to the actual needs of setting up the model of animal learning and memory behavior training method, existing labyrinth device still need improve.
Summary of the invention
The objective of the invention is for the labyrinth device of the more rational mouse memory training of a kind of design system is provided, overcome the deficiency of the inclined to one side lateral dominance in existing labyrinth device such as Y-maze, the T-maze detection.In addition, removed the setting that gradient voltage drives; And prevent that effectively animal from relying on smell to finish experiment, weighting Morris water maze and Y-maze, non-irrigated labyrinth strong point separately; Can detect according to the program control starlight that carries out of laboratory animal noctural habit; Can make laboratory animal that the sensory stimuli of the true environment placed oneself in the midst of is arranged, be more conducive to measuring of experimental data.
Technical scheme of the present invention is: a kind of closed rotary labyrinth device that is used for animal memory training system, comprise round box and circuit control system, some dividing plates are set between cabinet wall and the center column, thereby described casing is separated into the plurality of data acquisition zone, the purpose district, each dividing plate bottom is provided with the identical door opening of outward appearance, its technical essential is: the bottom half setting of described labyrinth device is by motor-driven rotating disk, described motor is arranged on the center column, and its output shaft axially is fastenedly connected with described rotating disk along center column, then described rotating disk rotates around described center column under described driven by motor, and described casing frame lower periphery is provided with the pulley of the described rotating disk of some supports; Described rotating disk top is provided with sieve shape supporting network; Described rotating disk below is provided with tank and forms the collecting dung treating apparatus, and described tank upper edge periphery has water pipe, evenly offers mouth spray on the described water pipe, and described bottom of gullet is provided with the bracing frame and the following mouth of a river, is provided with the water brush in the described tank; Described casing frame below is provided with the support of pulley; The described dividing plate in each interval is provided with liquid crystal display; Described purpose district is provided with food groove.
Each door opening place of described dividing plate below is provided with two pairs of photoelectricity correlation sensors, places respectively behind the door in front of the door, with the form output of ' or ', thereby detects the direction that mouse is moved into one's husband's household upon marriage.
Described food groove is the drum type that the side has grid, the round sides periphery of described food groove is provided with ring-shaped jacket, described overcoat is divided into two, and can separately expose grid down, by described circuit control system control, when overcoat is opened, animal is promptly had food as the award of finishing training mission from the grid below of described food groove, and after power supply disconnected, described overcoat was closed, seal described grid, animal promptly can't be had food.
Described dividing plate number is 6, and described round box equal angles is separated into A, B, C, D, six intervals of E, F, and wherein 4 door openings that equidistantly distribute are distinguished in 5 of data collection zone, 1 in purpose district below each described dividing plate.
Circuit control system comprises power supply, single-chip microcomputer, looks the sense of smell controller, flush discharge system controller automatically; Described automatic flush discharge system controller receives after described single-chip microcomputer sends control signal, the motor-driven valve that its electric valve controller control is installed on the described collecting dung treating apparatus opens or cuts out, the water pipe that the tank upper edge of described collecting dung treating apparatus is arranged is communicated with the water source or intercepts, regularly wash described tank, sewage flows out from described water channel sewage mouth; Described single-chip microcomputer sends a signal to the motor of the described rotating disk of control simultaneously, realizes rotatablely moving of rotating disk; The described sense of smell controller of looking is controlled liquid crystal display LCD and smell controller simultaneously, described single-chip microcomputer is after detecting scent signal, described smell controller is sent to the mechanical arm of training system by air compressor, control valve, smell generator, pressure-reducing valve with fresh air, is delivered to the smell jar that is provided with in the position, labyrinth by mechanical arm again.
The described water portion of brushing that is provided with in the described tank is fixed on described rotating disk bottom, and its underpart brush body contact with trench bottom with described tank sidewall, and in the time of described dial rotation, described water is brushed rotation thereupon, and then the bottom brush body can be scrubbed described tank.
Advantage of the present invention and positive technique effect: the present invention is on the basis of the advantage of fully receiving classical labyrinth, also have following characteristics: 1, be rotating disk rotation circular design by exquisiteness, make that every the wheel need be with the tested starting point of putting back between the experiment pilot scale time, can interruptedly not finish whole wheel experiment like this, overcome the deficiency of the inclined to one side lateral dominance of animal in Y-maze, T-maze detect to a certain extent; 2, the automatic flush discharge system that is provided with of labyrinth channel (door opening) can prevent effectively that animal from relying on smell to finish experiment, weighting Morris water maze and Y-maze, non-irrigated labyrinth (Barnes labyrinth) strong point separately; When 3, the present invention utilizes calculator to set up image, automatic data collection and analytical system, realized remote monitoring according to access rights, detected according to the program control starlight that carries out of laboratory animal noctural habit by means of LAN and Internet; 4, visual stimulus unit and smell releasing device synchronous working can make laboratory animal that the sensory stimuli of the true environment placed oneself in the midst of is arranged; Therefore, when the present invention estimates the animal subject ability of learning and memory: adopt the rotation of mouse autokinetic movement and chassis to drive the motivation that is integrated as the animal subject exploratory response, fasting or non-fasting mouse all are suitable for, meet the reality of animal normal physiological behaviouristics activity; Program control test at night: regularly rotation (1-2h at interval) and image, automatic data collection, utilize the nature of mouse nocturnalism and exploration to observe and detect, to animal stress be less, so can reduce the Confounding Factor of experimental result to a certain extent.Pre-adaptation stage reach: tested animal the raising stage just live in the duplicate labyrinth of detection system outward appearance in, allow it conform, rather than when experiment, just take out and make it be familiar with environment from the wean beginning.The equal outstanding behaviours of above characteristics this labyrinth device advantage that laboratory animal is carried out memory training, its experimental data is more accurate, experiment effect is even more ideal.
Description of drawings
The invention will be further described in conjunction with the accompanying drawings:
Fig. 1 is a concrete structure schematic diagram of the present invention;
Fig. 2 is the vertical view of Fig. 1;
Fig. 3 is a circuit control block diagram of the present invention;
Fig. 4 is the control block diagram of the automatic flush discharge of the present invention system;
Fig. 5 is the control block diagram of smell releasing device of the present invention;
Fig. 6 is the sink structure schematic diagram of collecting dung treating apparatus of the present invention;
Fig. 7 is the functional block diagram of application mouse memory training of the present invention system;
Sequence number explanation among the figure: 1 casing, 2 dividing plates, 3 liquid crystal displays, 4 motors, 5 sieve shape supporting networks, 6 rotating disks, 7 water pipes, 8 tanks, 9 water brush, 10 support pulleys, 11 supports, 12 pulleys, 13 door openings, 14 food grooves, 15 mouth sprays, 16 times mouths of a river.
Embodiment
Mouse memory training of the present invention system is a kind of device of detection of biological mouse spatial memory ability, system adopts circular camera bellows formula labyrinth as the behavioral data harvester, the closed-loop path that allows experimental mouse form by door opening, finish circuit training successively, utilize the autokinetic movement of mouse and the passive movement of stimulation to write down the movement track of mouse, gather this trace information by data acquisition unit.Be transferred to database in the Control System of Microcomputer by data/address bus then, control and monitor the crawler behavior of mouse at any time.
One, couple the present invention does concrete introduction according to Fig. 1-6: this is used for the closed rotary labyrinth device of animal memory training system, comprise round box 1 and circuit control system, some dividing plates 2 are set between casing 1 inwall and the center column, thereby casing is separated into the plurality of data acquisition zone, the purpose district, each dividing plate 2 bottom identical door opening 13 of outward appearance that equidistantly distributes, as shown in Figure 1, the dividing plate number is 6, and the round box equal angles is separated into A, B, C, D, E, six intervals of F, wherein data collection zone is 5,1 in purpose district, each dividing plate 2 below is provided with 4 door openings 13.
Wherein: casing 1 bottom is provided with rotating disk 6, center column is provided with motor 4, motor 4 output shafts axially are fastenedly connected with rotating disk 6 centers along center column, and then around the center column rotation, casing frame lower periphery is provided with the pulley 12 of some supporting wheels to rotating disk 6 under motor 4 drives; Rotating disk 6 tops are provided with sieve shape supporting network 5, can be wire netting or plastics system net etc.; Rotating disk 6 belows are provided with tank 8 and form the collecting dung treating apparatus, and tank 8 upper edge periphery have water pipe 7, evenly offer mouth spray 15 on the water pipe 7, and tank 8 bottoms are provided with the bracing frame and the following mouth of a river 16, also are provided with water brush 9 in the tank 8; Casing frame below is provided with the support 11 of pulley 10; Each interval dividing plate 2 is provided with liquid crystal display 3; The purpose district is provided with food groove 14.
The application's circuit control system comprises power supply, single-chip microcomputer, looks the sense of smell controller, flush discharge system controller etc. automatically.Single-chip microcomputer sends a signal to the motor 4 of control rotating disk, realizes rotatablely moving of rotating disk 6.The application's Electric Machine Control adopts solid-state relay control, the power<100w of drive motors.Solid-state relay adopts 1000w's herein.Single-chip microcomputer is managed indirect control relay by MOS.The instruction of Single-chip Controlling motor comes from host computer and sends the instruction of getting off by the CAN bus.CAN and RS232 conversion interface circuit are realized the data transaction between CAN bus protocol and the RS232 agreement.The host computer instruction sends to each child node by this system.
The detection of mouse position: each door opening 13 place that dividing plate below is provided with are provided with two photoelectricity correlation sensors, place respectively behind the door in front of the door, with the form output of ' or ', thereby just can detect the direction that mouse is moved into one's husband's household upon marriage, so that the run trace of detection mouse.
Acousto-optic to mouse stimulates: by single-chip microcomputer IO mouth, the grid of control MOS pipe is realized the control to light emitting source (liquid crystal display) and sound generation source, can control the frequency of sounding and luminous intensity.Wherein, single-chip microcomputer connects the data message that main control unit sends as sub-receiving element, extracts command information from data message, and then sends sound stimulation, light stimulus.The control data that can extract stimulation from data message has luminous intensity, audible frequency etc.
The visual stimulus unit of mouse: adopt the ViSaGe visual stimulus generator, belong to prior art.ViSaGe adopts 14bitDCAs image export technique, thereby visual stimulus color of image, the brightness of guaranteeing ViSaGe control output accurately and accurately show the time, even also the frame losing phenomenon can not take place when image switches fast.CRS MATLAB kit makes and adopts MATLAB Programming with Pascal Language output visual stimulus image to become more convenient.Simultaneously, multiple interfaces guarantee ViSaGe can with other device synchronization uses, as ResonseBox, EyeTracker, EEG, Optotrak and fMRI etc.
Food groove 14 has the drum type of grid for the side, and the one bottom surface can be attached on the smooth separator face.On another bottom surface on the other side, the opening of food groove is arranged, utilize folding semicircular upper half plane, food can be put into food groove.The round sides periphery of food groove is provided with ring-shaped jacket, overcoat is divided into two, under can separately expose grid, by circuit control system control, when overcoat is opened, animal can be had food as the award of finishing training mission from the grid below of food groove, after through certain hour (10s), power supply disconnects, and then overcoat is closed, seal grid, animal promptly can't be had food.
Automatically flush discharge system (dung collection system) controller receives AT89S51 single-chip microcomputer P3.6 (I/O) and sends control signal, control its BFC-2 type electric valve controller work, and then control the electronic motor-driven valve that is installed on the described collecting dung treating apparatus and open/close, the water pipe 7 that tank 8 upper edges of collecting dung treating apparatus are arranged is communicated with the water source or intercepts, the running water water source by motor-driven valve to launching gear, be that tank regularly washes, sewage flows out from the described mouth of a river down.The water that is provided with in the tank 8 is brushed 9 tops and is fixed on rotating disk 6 bottoms, its underpart brush body contacts with trench bottom with the tank sidewall, in the time of the Single-chip Controlling dial rotation, water brush 9 rotates thereupon, then the bottom brush body can be scrubbed tank, dispose the excreta of realization animal etc., and then eliminate the influence that smell brings experiment effect.
The smell releasing device: single-chip microcomputer is controlled liquid crystal display LCD3 and smell controller simultaneously by looking the sense of smell controller, single-chip microcomputer is after detecting scent signal, by air compressor, control valve, smell generator, pressure-reducing valve fresh air is sent to the mechanical arm that training system is provided with, is delivered to the smell jar that is provided with in the position, labyrinth by mechanical arm again.
Two, the application's network enabled operation, can realize the setting and the operation of remote control system:
Client/server (Client/Server) pattern:Information system under the early stage C/S model is mainly to be that a complete database application system is divided into foreground client computer (front end), application program and three major parts of background server, and realize interconnecting the transmission of the line data of going forward side by side between application program and the server by network.The core concept of this structure is exactly that client applications only needs to send request of data to server, and other every operation is all handled by server, and the result that will handle turns back in the middle of the application program of client computer at last.The processing procedure that this moment, server carried out is sightless fully to client applications.Database server and client computer are shared out the work and help one another, the distributed application task of finishing jointly.
When the main feature of two-layer client/server configuration is Processing tasks, to divide the work between the client-server, such advantage is embodied in: when client computer when server sends request, server only need be finished data query task that client computer sends and the result is returned, and so just can reduce the offered load amount of work station.Because the just request of data of client computer of transmission over networks and the result that server returns, it no longer is the entire database file, so just can allow the offered load of work station obviously diminish, promote the operating efficiency of the remote client of low speed connection effectively.
Because database adopts the centralized management pattern usually, client computer is by the sql like language accessing database time, and the platform of client computer can be again various, such as PC, Macintosh, UNIX work station or the like; And operating system also can be various, such as Windows, UNIX, 0S/2 or the like.Work as database structure like this and change, when perhaps database platform is changed, CLIENT PROGRAM will not need or only just need carry out the change of minute quantity.We roughly can draw by these characteristics, and database platform and work station are irrelevant, the conclusion that database and client program are irrelevant.Database has adopted the RDBMS based on affairs, keeps transaction journal and database backup/restoration mechanism, so just can carry out the maintenance of database well, in order to guarantee the integrality of database.
The database application exploitation can be divided into three parts on logical meaning: data presentation layer, logic application layer and data service layer.Above-mentioned traditional two-layer client/server configuration can laterally expand, thereby just can obtain three layers or more multi-layered client/server configuration.
Communication protocol:Because the uncertainty of communication process, in order to ensure the error free transmission of data, our final decision adopt stop-etc. agreement and in communication process, carry out CRC check.Stop-and-wait protocol: after receiving terminal is correctly received data, return an acknowledgement frame (ACK), transmitting terminal just sends next data.After receiving terminal is received misdata, return a negative frame (NAK), transmitting terminal retransmits.After loss of data, transmitting terminal can carry out overtime re-transmission.So just guaranteed that transmitting terminal and receiving terminal set up zero defect and be connected.For the correctness of checking data, we adopt the method for CRC check, adopt 8 bit check sign indicating numbers.Therefore our definition frame length is 24,3 bytes.First byte is a control bit, and second byte is data bit, and the 3rd byte is the CRC check position.Communicate by letter that we adopt the mode of token bus, master unit scan round subelement between 22 Control Node and the main communication node.When some subelements when event occurs, this subelement captures bus and master unit is realized point to-point communication.
Three, to the simple description (disposing corresponding stimulating apparatus with following concrete labyrinth device is example) of the training system that utilizes this labyrinth device:
The box shape physical dimension of labyrinth device is diameter 200cm, high 80cm.System space is separated uniformly by 6 dividing plates, evenly distributes 4 in the bottom of every dividing plate and passes through hole (door opening).2 pairs of infrared photoelectric sensors are established at every hole.The bottom is that the iron wire structure of mesh-like can be used to support experimental mouse or the motion thereon of machine mouse, is provided with motor simultaneously at system centre axle place and rotates, and can drive the rotation of screen cloth.Like this, put into the experimental mouse of system or machine mouse and just pass through between the hole back and forth motion under these 6 dividing plates, meanwhile, screen cloth has constituted the forms of motion of whole system also in the rotation that does not stop.Pass through high 90 mm in hole, wide 110 mm, thick 60 mm, 100 mm that the hole spacing is, the hole dimension of bottom surface screen cloth are 12 x, 12 mm.This system is by the Electric Machine Control dial rotation and then force the experimental mouse training of moving into one's husband's household upon marriage.The function of this training system realizes as shown in Figure 7.
, training system rule set: all to carry out the setting of rule before testing at every turn, rule comprises the prime area of experimental mouse, the attribute of each (representing whether being of passability respectively) as Lv Men and red door, and look experimental mouse and finish training and give single or the combination rewards and punishments.
A) prime area is set: set the prime area of experimental mouse, convenient later statistical work.
B) Hong Men, green door are set: set the attribute of 24 doors, i.e. passability.
C) stimulate kind to set: to stimulate kind to comprise stimulations such as sound, light, electricity, water, gas, food, image and smell.When experimental mouse is passed through non-green door (red door), give and different punitive measures, or when experimental mouse is successfully passed through green, give and different incentive measures.
Native system can be realized multiple stimulation by mechanical arm, remote control stimulating apparatus.Three kinds of terminal mechanisms that stimulate wherein are set on the mechanical arm, the terminal mechanical arm that stimulates mechanism for subsidiary three kinds of pipelines, three kinds of pipeline corresponding water, gas, food stimuluss respectively, as shown in Figure 8, waterpipe, gas passage, passage of food are set respectively on the mechanical arm, and the mechanical arm end also is provided with the mobile liquid crystal screen.Stimulating system realizes controlling the supply of water, gas, food respectively by the PLC control system, and behind the position that navigates to experimental mouse, the terminal magnetic valve that is provided with of mechanical arm is opened automatically according to the mistakes gate signal that PC sends, and discharges stimulation accordingly.
The remote control stimulating apparatus comprises compositions such as electro photoluminescence module, mechanical stimulus module, sound stimulating module, relay driving module, wireless receiving module and wireless transmitter module, after fitting together, above-mentioned electro photoluminescence, sound stimulation, Mechanical vibration stimulation module, wireless transmitter module, wireless receiving module and relay driving module be fixed on the carrier of laboratory animal health outside binding, this carrier can be resilient canvas, and the canvas two ends are provided with bonding and buckle so that bundle experimental mouse.When experimental mouse detects beginning in the path exercising model of mouse and intelligent behavior monitoring system after, be banded in reception on the experimental mouse back, stimulating apparatus in experimental mouse during, the stimulation of setting by non-green door (red door).
D) stimulation time is set: the stimulation time when at every turn stimulating, also can select after stimulating beginning, manually to stop to stimulate.
E) chassis speed setting: the rotating speed that the chassis is set.
2, data acquisition: whenever carry out taking turns test and all will gather by the information of door to experimental mouse.
A) single-wheel route statistics: experimental mouse is from the zone of departure, gets back to the zone of departure at last and is defined as one and takes turns.The route of process is taken turns in this function statistical experiment mouse walking one.
B) overall success and frequency of failure statistics: experimental mouse is finished a circle by the green door and is defined as success, has passed through red door and has been defined as failure, the number of times of this function statistical experiment mouse success and failure.
C) Hong Men, green door are added up by situation: this function statistical experiment mouse is by the number of times of Hong Men and green door.
3, process shows: the position of attribute of each and experimental mouse can show intuitively in this system.
A) Hong Men, green door show: each attribute display is come out in the difference by each color.
B) the experimental mouse motion process shows: show the current position of experimental mouse.
4, document management: will add up the information that obtains and generate backup document.
A) generate statistical report form: statistical information is exported in the Excel form.
B) generation system daily record: this system journal will comprise that host computer is subjected to and the time and the content of each bar order of sending.(comprising when what door when experimental mouse cross, be subjected to which type of stimulation etc.)
When mouse moved on to experimental mouse, it is capable that mouse pointer becomes cross, and can drag experimental mouse and set the prime area this moment.
This experimental system software adopts the C# programming language to finish the work of writing of software in conjunction with the .NET framework, and translation and compiling environment adopts Visual Studio 2008.Database adopts the MySQL database.
It is desirable more that the present invention disposes the animal memory training system experimentation effect that above-mentioned stimulating apparatus forms, and the research in fields such as neuro-physiology, Neuropharmacology and psychology is had unusual meaning.

Claims (6)

1. closed rotary labyrinth device that is used for animal memory training system, comprise round box and circuit control system, some dividing plates are set between cabinet wall and the center column, thereby described casing is separated into plurality of data acquisition zone, purpose district, each dividing plate bottom is provided with the identical door opening of outward appearance
It is characterized in that: the bottom half setting of described labyrinth device is by motor-driven rotating disk, described motor is arranged on the center column, and its output shaft axially is fastenedly connected with described rotating disk along center column, then described rotating disk rotates around described center column under described driven by motor, and described casing frame lower periphery is provided with the pulley of the described rotating disk of some supports;
Described rotating disk top is provided with sieve shape supporting network;
Described rotating disk below is provided with tank and forms the collecting dung treating apparatus, and described tank upper edge periphery has water pipe, evenly offers mouth spray on the described water pipe, and described bottom of gullet is provided with the bracing frame and the following mouth of a river, is provided with the water brush in the described tank;
Described casing frame below is provided with the support of pulley;
The described dividing plate in each interval is provided with liquid crystal display;
Described purpose district is provided with food groove.
2. the closed rotary labyrinth device that is used for animal memory training system according to claim 1, it is characterized in that: each door opening place of described dividing plate below is provided with two pairs of photoelectricity correlation sensors, place respectively behind the door in front of the door,, thereby detect the direction that mouse is moved into one's husband's household upon marriage with the form output of ' or '.
3. the closed rotary labyrinth device that is used for animal memory training system according to claim 1, it is characterized in that: described food groove is the drum type that the side has grid, the round sides periphery of described food groove is provided with ring-shaped jacket, described overcoat is divided into two, under can separately expose grid, by described circuit control system control, when overcoat is opened, animal is promptly had food as the award of finishing training mission from the grid below of described food groove, after power supply disconnects, described overcoat is closed, and seals described grid, and animal promptly can't be had food.
4. the closed rotary labyrinth device that is used for animal memory training system according to claim 1, it is characterized in that: described dividing plate number is 6, and described round box equal angles is separated into A, B, C, D, six intervals of E, F, wherein 4 door openings that equidistantly distribute are distinguished in 5 of data collection zone, 1 in purpose district below each described dividing plate.
5. the closed rotary labyrinth device that is used for animal memory training system according to claim 1 is characterized in that: circuit control system comprises power supply, single-chip microcomputer, looks the sense of smell controller, flush discharge system controller automatically;
Described automatic flush discharge system controller receives after described single-chip microcomputer sends control signal, the motor-driven valve that its electric valve controller control is installed on the described collecting dung treating apparatus opens or cuts out, the water pipe that the tank upper edge of described collecting dung treating apparatus is arranged is communicated with the water source or intercepts, regularly wash described tank, sewage flows out from described water channel sewage mouth;
Described single-chip microcomputer sends a signal to the motor of the described rotating disk of control simultaneously, realizes rotatablely moving of rotating disk;
The described sense of smell controller of looking is controlled liquid crystal display LCD and smell controller simultaneously, described single-chip microcomputer is after detecting scent signal, described smell controller is sent to the mechanical arm of training system by air compressor, control valve, smell generator, pressure-reducing valve with fresh air, is delivered to the smell jar that is provided with in the position, labyrinth by mechanical arm again.
6. the closed rotary labyrinth device that is used for animal memory training system according to claim 1, it is characterized in that: the described water portion of brushing that is provided with in the described tank is fixed on described rotating disk bottom, its underpart brush body contacts with trench bottom with described tank sidewall, in the time of described dial rotation, described water brush rotates thereupon, and then the bottom brush body can be scrubbed described tank.
CN201110052637.1A 2011-03-07 2011-03-07 Closed rotary maze device for animal memory training system Expired - Fee Related CN102144572B (en)

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