WO2012119322A1

WO2012119322A1 - Closed rotary maze device for animal memory training system

Info

Publication number: WO2012119322A1
Application number: PCT/CN2011/071865
Authority: WO
Inventors: 朱启文; 曹伟平; 赵建文; 商丽宏; 匡宝平; 王克威; 李玉生
Original assignee: 沈阳医学院
Priority date: 2011-03-07
Filing date: 2011-03-16
Publication date: 2012-09-13
Also published as: CN102144572A; GB2498685A; GB201309005D0; GB2498685B; CN102144572B

Abstract

A closed rotary maze for animal memory training system includes a round case (1) and a circuit control system. The bottom of the case (1) is provided with a rotary plate (6) actuated by a motor (4). The motor (4) is installed on a central post, and the output shaft of the motor (4) is fixedly connected with the rotary plate (6) along the axis of the central post so that the rotary plate (6) is rotated around the central post by the action of the motor (4). The lower peripheral of the frame of the case (1) is provided with several pulleys (12) for supporting the rotary plate (6). A screen-like support net (5) is provided on the rotary plate (6). The present maze adopts the design of using a rotary plate to rotate the round case so that the experimental animals need not be put back to starting-point and the whole session of experiment can be completed uninterruptedly. The maze device abandons the configuration of maze being driven by gradient voltage in prior art. The maze is equipped with an automatic flush and drainage system so as to effectively prevent animals from depending on smell to complete a test. The maze is provided with a remote monitor so as to perform detection under control of programs according to living behaviors of the experimental animals at night. A visual stimulation unit and an odor release unit in the maze work simultaneously, such that the experimental animals feel stimulation as if in a real environment, which facilitates acquirement of the experimental data.

Description

Closed rotary labyrinth device for animal memory training system

[Technical Field]

The present invention relates to a model for establishing a training method for animal learning and memory behavior, and more particularly to a closed rotary maze device for an animal memory training system. It can be applied to fields such as neurophysiology, neuropharmacology and psychology.

【Background technique】

At present, animal behavior experiments are an indispensable and important means to study advanced brain function and other neurosciences, especially in behavioral medical research such as neurophysiology and neuropharmacology. The labyrinth experiment is a classic project of behavioral experiments. It is one of the most commonly used experiments to study the learning and memory function of rats (or mice), and it is also a common animal model for cognitive drug screening.

In the maze task, the instrument of the maze device itself tells the animal what they need to do (similar to the instructions for human experiments). In this task, researchers generally refer to the concepts of working memory and reference memory. To complete a task for an animal, the first thing you need is a reference memory, which reflects the animal's learning of information about the Trial in the task (such as the position of the spatial cues, the appearance of the maze, and the experimental rules), so the animal is forming. In the process of referring to memory, reference information should exist during each experiment. In a laboratory setting, the working memory of an animal is the ability to retain information between different trials in each session of the experiment. In comparison, working memory is different between trials, and it is the ability of animals to maintain the information of each trial (where they have just arrived). After a trial, the rats were able to erase this particular working memory, but kept the reference memory unchanged. Therefore, working memory only works in one trial, and reference memory is long-term memory.

The existing maze devices mainly include the following: 1. Drought maze measures animal working memory model: Drought maze mainly refers to various radial maze, Y maze, Τ maze and so on. The Υ, Τ-type labyrinth and Barnes labyrinth experiment did not provide reward and punishment conditions. It is entirely based on the nature of animal exploration. This kind of maze task is to learn the memory driven by animal hunger motivation, so the animal's food intake should be strictly controlled within one week before the experiment. , to reduce its body mass to about 85%. However, the problem with this method is that the non-learning factors such as the animal's walking around the arm and the degree of hunger have an impact on the experimental results.

2, water maze measurement animal working memory model: The classic water maze model is the Morris water maze (MWM), using animals to avoid flooding and looking for a platform to escape the vivid machine for learning and memory tasks. In addition, on the basis of the drought maze, water can be added to the experimental device to form an 8-arm radiant water maze or a T-water maze, a Y-water maze, etc., to eliminate the influence of odor cues on the experimental results. When using the water maze for behavioral testing, there is no need to control the animal's diet; the animal training takes less time, about 1 week; the computer can be used to create an automatic image acquisition and analysis system, which can be made based on the collected data. The corresponding histogram and running trajectory map are convenient for researchers to further analyze and discuss the experimental results. However, the disadvantage of MWM is that the incubation period of animal swimming is closely related to its motor function state, so the drugs that affect the animal's motor function and the state of the animal itself may affect the normal results of the experiment. In addition, due to the need for monitoring systems and analysis software, only a few well-equipped laboratories can work. The design of the experimental program needs to consider many factors, and at the same time, the experimenter needs to have certain knowledge of neurophysiology, cognitive physiology and mathematical statistics, which brings difficulties to the development of the experiment and the interpretation of the results, and also limits the maze. Intensive and extensive application; due to too much physical exertion and excessive loss of body temperature, it is difficult to complete the task of old and weak rats; not all mouse strains are suitable for Morris maze test, such as BALB/c can not learn the task (the score does not follow The number of days increases and the academic performance of 129/SvJ is also biased; the scores between individuals vary greatly; some strains, such as the 129/SvJ strain, have an age-related visual pathological lesion, which is visualized during aging. Difficulties arise in basic learning and memory tasks. In C57BL/6 strain, due to severe alopecia, some mice may cause depression, and ulcerative dermatitis may eventually affect the swimming ability of mice and affect the experimental results. The labyrinth procedure may be insensitive to a slight loss of learning and memory; immersing the animal in water may cause internal Secretion or other stress effects. The interaction between the latter and brain damage or pharmacological operations is uncertain; the operation of the automated surveillance system still needs to be done by hand, which may make the experiment somewhat boring; The experimental site is too large. 3. Other mouse behavior models: One-time passive avoidance and jumping (or climbing) models are two simple models for studying learning and memory, which are suitable for studying memory retention and memory consolidation. Both models record how long the animal does not enter the chamber after the electric shock or does not jump down the platform to test the retention and consolidation of the memory; the new object recognition experiment is a measure of the animal's non-space working memory ability. The model takes advantage of the principle that animals innately explore new objects. During the test period, if the animal remembers the old object, the animal has less time to explore the old object than the new one.

In summary, the above-mentioned classic labyrinth devices still have different degrees of limitations, many human factors, cumbersome technical operations, and lack of comprehensive influencing factors such as electricity, sound (noise) and light. In order to solve the above problems, the applicant has continuously researched and disclosed the technical proposal of the patent application number "CN200610045868.9": a mouse auditory, visual memory model and intelligent behavior detecting system, the mouse's auditory and visual memory The model includes a labyrinth with a data acquisition area, a destination area, and a connecting channel thereof. The labyrinth uses a circular dark box maze with a multi-channel closed loop, and in the box with the upper cover and the bottom of the fence, the inside of the box is passed through the partition The cavity is divided into at least three test zones, one of which is the target zone, and the remaining data collection zone, the bottom of each baffle is equally spaced, and has the same shape of a hole-shaped channel with an adjustable one-way door, and each hole-shaped access door side There are two sets of infrared emitting and receiving optoelectronic components, and each of the isolated zones is provided with controllable lighting. The bottoms of each zone are respectively provided with randomly controlled electric shock conductors for generating different gradient voltage bands, in the upper control box of the middle column of the box. Set the glare generator, noise generator and video tracking head. The auditory and visual memory model utilizes controllable lighting to adapt to the mouse's darkening habit, allowing it to perform autonomous motion in the box, combined with different gradient voltage bands generated by the electric shock conductor, and driven by electric shock stimulation to randomly control the passive movement of the rat. Suitable for fasting and non-fasting rats. Different from the training of the existing water maze for forcing the animal to swim, the combination of the autonomous movement of the rat's natural activity mode and the passive driving of the gradient voltage prevents the rats from receiving a single electrical stimulation and stay away from the designated channel, avoiding the model animals staying in the data collection device. Inconsistent time makes time-class data more accurate. The infrared emission and receiving optoelectronic components on the side of each channel of the labyrinth can detect the whereabouts of the animal in the labyrinth in real time and accurately. However, the Applicant has found that the labyrinth model still has the following disadvantages: The electric shock stimulation is not sufficiently gentle to drive, and the passive driving of the gradient voltage is easy to cause different degrees of damage to the experimental animals, and the setting is complicated; in addition, the device is not perfect, the experimental animals The experiment may be completed by odor, and the above shortcomings will affect the experimental results and affect the accuracy of the experimental data.

In summary, the existing maze device still needs improvement based on the actual needs of the model for establishing animal learning and memory behavior training methods. [Summary of the Invention]

The object of the present invention is to provide a labyrinth device for designing a more reasonable mouse memory training system, which overcomes the disadvantages of the lateral advantages of the existing labyrinth devices such as Y-maze and T-maze. In addition, the gradient voltage-driven setting is removed; and the animal is effectively prevented from completing the experiment by odor, and the advantages of the Morris water maze and the Y-ma _Ze and the drought maze are weighted; the starlight detection can be performed according to the nighttime activity of the experimental animal; The experimental animals have the sensory stimulation of being in the real environment, which is more conducive to the measurement of experimental data.

The technical solution of the present invention is: a closed rotary labyrinth device for an animal memory training system, comprising a circular box body and a circuit control system, wherein a plurality of partition plates are disposed between the inner wall of the box body and the center cylinder body, thereby The box body is divided into a plurality of data collection areas and a destination area, and the bottom of each partition plate is provided with the same door opening. The technical point is that: the bottom of the box body of the labyrinth device is provided with a motor-driven turntable, and the motor is disposed on the center column. Body, and the output shaft is fastened to the turntable along the axial direction of the central cylinder, the turntable is rotated around the central cylinder by the motor, and a plurality of supports are arranged around the lower part of the frame a pulley of the turntable; a sieve-shaped support net is arranged above the turntable; a sink is formed under the turntable to form a manure collection and processing device, and a water pipe is arranged along the periphery of the water tank, and the spray pipe is hooked on the water pipe a bottom of the water tank is provided with a support frame and a water outlet, wherein the water tank is provided with a water brush; a bracket with a pulley is arranged below the frame of the box; Said separator plate is provided with the liquid crystal panel; the destination area with the feed trough.

Two pairs of photoelectric radiation sensors are disposed at each door hole below the partition plate, and the front door is respectively placed behind the door and outputted in the form of 'or' to detect the direction of the mouse passing the door.

The food trough is a drum type with a mesh on the side, the circular side of the food trough is provided with an annular outer casing, and the outer casing is divided into two, and the lower part can be separately exposed to the grid, and the circuit control system is Control, when the coat is opened, the animal eats food from under the grid of the food trough. In order to complete the reward of the training task, after the power is turned off, the jacket is closed, the grid is sealed, and the animal cannot eat the food.

The number of the partitions is 6, and the circular box is equally divided into six sections of A, B, C, D, E, and F, wherein the data collection area is 5 and the destination area is 1 each. Four door holes are equally spaced below the partition.

The circuit control system comprises a power supply, a single chip microcomputer, an eye slap controller, and an automatic flushing drainage system controller; after the automatic flushing drainage system controller receives the control signal from the single chip microcomputer, the electric valve controller controls the installation of the faeces The electric valve on the collection processing device is turned on or off, so that the water pipe disposed along the water tank of the fecal collecting and processing device is connected or blocked with the water source, and the water tank is periodically flushed, and the sewage flows out from the water outlet of the water tank; Sending a signal to the motor controlling the turntable to realize a rotary motion of the turntable; the visual sigh controller simultaneously controls the LCD screen and the scent controller, and after the single chip detects the squint signal, the odor controller passes The air compressor, control valve, scent generator, and pressure relief valve deliver fresh air to the robotic arm of the training system, which is then transported by the robotic arm to the scent canister located in the labyrinth position.

The upper part of the water brush disposed in the water tank is fixed at the bottom of the turntable, and the lower brush body is in contact with the side wall of the water tank and the bottom of the tank. When the turntable rotates, the water brush rotates accordingly, and the lower part The brush body can scrub the sink

Advantages and positive technical effects of the present invention: The present invention has the following features on the basis of fully absorbing the advantages of the classic labyrinth: 1. The circular design is rotated by a delicate turntable, so that the test is not between each round of experiments. The subject needs to be put back to the starting point, so that the whole round of experiments can be completed without interruption, which overcomes the deficiency of the animal's lateral advantage in Y-maz _e and T-maze detection to some extent; 2. Maze channel (door hole) The automatic flushing drainage system can effectively prevent animals from completing experiments with odor, and weights the advantages of Morris water maze and Y- _maZ e, drought labyrinth (Barnes labyrinth); 3. The invention uses computer to establish images, automatic data acquisition and When analyzing the system, the remote monitoring is realized by means of the local area network and the Internet according to the access authority, and the starlight detection is performed according to the nighttime activity habit of the experimental animal; 4. The visual stimulation unit and the scent release device work synchronously, so that the experimental animal can be placed in the real environment. Sensory stimulation; therefore, the present invention evaluates the ability of the test animal to learn and remember: using a mouse The combination of autonomous exercise and chassis rotation drive is the motivation for the test animals to explore the response. It is suitable for fasting or non-fasting rats. It is in line with the actual physiological behavior of animals. Night program control test: Timing rotation (interval l-2h) And the automatic acquisition of images and data, the use of mouse night activities and exploration of natural observation and detection, the animal's stress is small, so it can reduce the confounding factors of the experimental results to some extent. Advance in the pre-adaptation phase: The animals in the test phase live in the same maze as the detection system, so that they can adapt to the environment from the time of weaning, rather than taking it out of the environment to make them familiar with the environment. All of the above features highlight the advantages of the labyrinth device for memory training of experimental animals. The experimental data is more accurate and the experimental results are more ideal.

[Description of the Drawings]

The invention is further described in conjunction with the accompanying drawings:

Figure 1 is a schematic view of a specific structure of the present invention;

Figure 2 is a plan view of Figure 1;

Figure 3 is a block diagram of the circuit control of the present invention;

Figure 4 is a control block diagram of the automatic flushing drainage system of the present invention;

Figure 5 is a control block diagram of the scent release device of the present invention;

Figure 6 is a schematic view showing the structure of a water tank of the feces collection and treatment device of the present invention;

Figure 7 is a functional block diagram of a mouse memory training system to which the present invention is applied;

The serial number in the figure shows: 1 box, 2 partitions, 3 LCD screens, 4 motors, 5 mesh support nets, 6 turntables, 7 water pipes, 8 sinks, 9 water brushes, 10 bracket pulleys, 11 brackets, 12 pulleys, 13 Door hole, 14 food trough, 15 sprinklers, 16 sewers.

【detailed description】

The mouse memory training system of the present invention is a device for detecting the spatial memory ability of a biological mouse. The system adopts a circular dark box maze as a behavior data acquisition device, allowing the experimental mouse to complete the cycle training through the closed loop formed by the door hole. Rat's autonomous movement and passive stimulation The action record of the mouse is recorded by the motion, and the track information is collected by the data acquisition device. Then, it is transmitted to the database in the microcomputer control system through the data bus, and the activity behavior of the mouse is controlled and monitored at any time.

1. The present invention is specifically described with reference to Figures 1-6: The closed rotary labyrinth device for an animal memory training system includes a circular box 1 and a circuit control system, and a plurality of between the inner wall of the box 1 and the center cylinder The partition 2 divides the box into a plurality of data collection areas and destination areas, and the bottom of each partition 2 is equidistantly distributed with the same appearance of the door opening 13, as shown in FIG. 1, the number of the partitions is 6, and the circular box The body is equally divided into six intervals of in, B, C, D, E, and F, of which 5 are data acquisition areas and 1 is a destination area, and 4 door openings 13 are disposed under each partition 2.

Wherein: the bottom of the box body 1 is provided with a turntable 6, and the central column body is provided with a motor 4, and the output shaft of the motor 4 is fastened and connected to the center of the turntable 6 along the axial direction of the center cylinder body, and the turntable 6 is rotated around the center cylinder by the motor 4 There are a plurality of pulleys 12 supporting the turntable around the lower part of the frame frame; a sieve-shaped support net 5 is arranged above the turntable 6, which may be a metal mesh or a plastic mesh; and a sink 8 is formed under the turntable 6 to form a manure collection and processing device, a sink 8 is arranged with a water pipe 7 along the periphery, a water spray pipe 15 is provided on the water pipe 7, a support frame and a water outlet 16 are arranged at the bottom of the water tank 8, and a water brush 9 is also arranged in the water tank 8; a pulley is arranged under the frame of the tank The bracket 11 of 10; the partition 2 of each section is provided with a liquid crystal panel 3; the target area is provided with a food tank 14.

The circuit control system of the present application includes a power supply, a single chip microcomputer, an eye slap controller, an automatic flushing drainage system controller, and the like. The MCU sends a signal to the motor 4 of the control carousel to realize the rotary motion of the carousel 6. The motor control of this application is controlled by solid state relay, and the power of the drive motor is <100w. The solid state relay in this paper uses lOOOw. The microcontroller indirectly controls the relay through the MOS transistor. The instruction of the MCU to control the motor comes from the instruction sent by the host computer through the CAN bus. The CAN and RS232 conversion interface circuits implement data conversion between the CAN bus protocol and the RS232 protocol. The upper computer command is sent to each child node through the system.

Detection of the position of the mouse: Two photoelectric radiation sensors are arranged at each door opening 13 provided under the partition plate, and the front door of the door is placed separately and outputted in the form of 'or', so that the direction of the rat crossing the door can be detected, so as to detect The walking path of the mouse.

Sound and light stimulation of the mouse: The gate of the MOS tube is controlled by the MCU 10 port, and the control of the illumination source (liquid crystal panel) and the sound source is realized, and the frequency of the sound generation and the intensity of the light emission can be controlled. Wherein, the single-chip microcomputer is used as the sub-receiving unit to receive the data information transmitted by the main control unit, and extracts the command information from the data information, thereby generating the sound stimulation and the light stimulation. The control data from which the stimulus can be extracted from the data information has luminous intensity, vocalization frequency, and the like.

Mouse visual stimulation unit: The ViSaGe visual stimulation generator is a prior art. ViSaGe uses 14bit DCAs image output technology to ensure that ViSaGe controls the output of visually stimulating image color, brightness and accurate display time, even when the image is quickly switched, no frame loss occurs. The CRS MATLAB toolkit makes it easier and faster to program visually stimulated images in MATLAB. At the same time, multiple interfaces ensure that ViSaGe can be used in sync with other devices such as ResonseBox, EyeTracker, EEG, Optotrak and fMRI.

The food trough 14 is a drum type with a mesh on the side, and a bottom surface can be attached to a smooth partition surface. On the other bottom surface opposite thereto, there is an opening for the food trough, and the food can be placed in the food trough by using the upper half of the foldable semicircle. The circular side of the food trough has an annular outer casing. The outer casing is divided into two. The lower part can be separately exposed to the grid and controlled by the circuit control system. When the outer casing is opened, the animal can eat food from the bottom of the food trough. After completing the training task, after a certain period of time (10s), the power is turned off, the jacket is closed, the grid is sealed, and the animal cannot eat the food.

The automatic flushing drainage system (fecal collection system) controller receives the AT89S51 single-chip microcomputer P3.6 (I/0) to issue a control signal to control the operation of its BFC-2 type electric valve controller, and then controls the electric installation in the feces collection and processing device. The upper electric valve is opened/closed, so that the water pipe 7 disposed along the water tank 8 of the fecal collecting and processing device is connected or blocked with the water source, and the tap water source is flushed through the electric valve to the sewage device, that is, the water tank, and the sewage flows out from the water outlet. The upper part of the water brush 9 disposed in the water tank 8 is fixed at the bottom of the turntable 6, and the lower brush body is in contact with the side wall of the water tank and the bottom of the tank. When the single-chip microcomputer controls the turntable to rotate, the water brush 9 rotates accordingly, and the lower brush body can be opposite to the sink. The brushing is performed to remove the excrement of the animal, thereby eliminating the influence of the odor on the experimental effect.

Odor release device: The MCU controls the LCD screen LCD3 and the odor controller simultaneously through the squint controller, and the MCU detects the sigh letter. After the number, the fresh air is sent to the robot arm set by the training system through the air compressor, control valve, odor generator and pressure reducing valve, and then transported by the robot arm to the scent tank set in the labyrinth position.

Second, the application supports networked operation, which can realize the setting and operation of the remote control system:

I (Client Server) The information system in the early C / S mode is mainly to divide a complete database application system into three main parts: the front-end client (front end), the application and the back-end server, and implement the application through the network. Interconnection with the server and data transfer. The core idea of this structure is that the client application only needs to send data requests to the server, all other operations are processed by the server, and finally the processed results are returned to the client's application. The processing performed by the server at this point is completely invisible to the client application. The database server and the client work in a division of labor and distribute the application tasks together.

The main feature of the two-tier client/server architecture is that when the task is processed, the division of the client and the server is performed. This advantage is reflected in: When the client sends a request to the server, the server only needs to complete the data sent by the client. Querying the task and returning the results reduces the network load on the workstation. Because the data transmission on the network is only the client's data request and the processing result returned by the server, it is no longer the entire database file, which can make the network load of the workstation significantly smaller, effectively improving the working efficiency of the low-speed connected remote client.

Since the database usually adopts a centralized management mode, when the client accesses the database through the SQL language, the client platform can be diverse, such as PC, Macintosh, UNIX workstation, etc.; and the operating system can also be diverse, such as Windows, UNIX. , OS / 2 and more. This way, when the database structure changes, or the database platform changes, the client program will not need or just need to make very few changes. From these characteristics, we can roughly conclude that the database platform has nothing to do with the workstation, and the database is independent of the client program. The database uses a transaction-based RDBMS that preserves the transaction log and database backup/restore mechanism, which is a good way to maintain the database to ensure database integrity.

Database application development can be divided into three parts in a logical sense: the data presentation layer, the logical application layer, and the data service layer. The above traditional two-tier client/server architecture can be scaled horizontally to achieve a three- or more-tier client/server architecture.

Due to the uncertainty of the communication process, in order to ensure error-free transmission of data, we finally decided to adopt a stop-and-wait protocol and perform CRC check during the communication process. Stop protocol: After the receiver receives the data correctly, it returns an acknowledgement frame (ACK), and the sender sends the next data. When the receiving end receives the error data, it returns a negative frame (NAK) and the transmitting end retransmits. When the data is lost, the sender can perform a timeout retransmission. This ensures that the sender and the receiver establish an error-free connection. In order to verify the correctness of the data, we use the CRC check method and use an 8-bit check code. So we define the frame length to be 24 bits and 3 bytes. The first byte is the control bit, the second byte is the data bit, and the third byte is the CRC check bit. The communication between the 22 control nodes and the main communication node uses the token bus mode, and the main unit cyclically scans the subunits. When an event occurs in a subunit, the subunit occupies the bus and implements point-to-point communication with the main unit.

3. Brief description of the training system using the labyrinth device (for example, the following specific labyrinth device is configured with the corresponding stimulation device): The frame size of the labyrinth device is 200 cm in diameter and 80 cm in height. The system space is separated by six partitions, and four through holes (door holes) are distributed in the lower part of each partition. There are 2 pairs of infrared photoelectric sensors per hole. The bottom of the screen is a wire-like wire structure that can be used to support the movement of the test mouse or machine mouse, and a motor rotation at the central axis of the system will drive the screen to rotate. In this way, the experimental mouse or machine mouse placed in the system moves back and forth between the six holes under the diaphragm, and at the same time, the screen is also rotated continuously, forming the motion form of the entire system. The hole height is 90 mm, the width is 110 mm, the thickness is 60 mm, the hole spacing is 100 mm, and the bottom mesh has a hole size of 12 X 12 mm. The system forces the rats to undergo door training by rotating the motor control dial. The function realization of the training system is shown in Figure 7.

1. The rule setting of the training system: The rules should be set before each test. The rules include the initial area of the experiment mouse. The attributes of each door (such as the green door and the red door respectively represent the passability). No), and give the single or combined reward and punishment according to the completion of the training of the experimental rats.

a) Initial area setting: Set the initial area of the experiment mouse to facilitate future statistical work.

b) Red door, green door setting: Set the properties of 24 doors, you can pass the sex. c) Stimulus type setting: Stimulus types include stimuli such as sound, light, electricity, water, gas, food, images and odor. When the rats pass the non-green door (red door), different penalties are given, or when the rats successfully pass the green door, different incentives are given.

The system can achieve a variety of stimuli through the robot arm and remote control stimulation device. There are three kinds of end stimulation mechanisms on the mechanical arm, and the end stimulation mechanism is a mechanical arm with three kinds of pipes. The three pipes respectively correspond to water, gas and food stimulation. As shown in Fig. 8, the water pipes and gas are respectively arranged on the mechanical arms. The channel, the food channel, and the end of the arm also have a mobile LCD screen. The stimulation system controls the supply of water, gas and food through the PLC control system. When the position of the experimental mouse is positioned, the solenoid valve at the end of the robot arm is automatically opened according to the signal sent by the PC to release the corresponding stimulus.

The remote stimulation device comprises an electrical stimulation module, a mechanical stimulation module, an acoustic stimulation module, a relay driving module, a wireless receiving module and a wireless transmitting module, and the above electrical stimulation, acoustic stimulation, mechanical vibration stimulation module, wireless transmission module, wireless receiving module and The relay drive modules are assembled and fixed on a carrier bundled on the outside of the body of the experimental animal. The carrier may be a flexible canvas, and the ends of the canvas are provided with a fastening buckle to facilitate binding of the experimental mouse. When the rats were tested in the mouse path training model and the intelligent behavior monitoring system, the receiving and stimulating devices bundled on the back of the rats were given the set stimulation when the rats passed the non-green door (red door). .

d) Stimulus time setting: The stimulation time at each stimulation can also be selected to stop the stimulation manually after the stimulation starts.

e) Chassis speed setting: Set the speed of the chassis.

2. Data collection: Each time a round of testing is carried out, the information of the rats passing through the door is collected.

a) Single-round route statistics: The experimental mouse departs from the departure area, and finally returns to the departure area as a round. This function counts the route that the experimental mouse walks through one round.

b) Statistics of overall success and failure times: The experiment mouse is defined as success through the green door, and the red door is defined as failure. This function counts the number of successes and failures of the experimental mouse.

c) Red door and green door pass statistics: This function counts the number of times the experimental mouse passes the red door and the green door.

3. Process display: The properties of each door in the system and the position of the experimental mouse can be displayed visually.

a) Red door and green door display: The attributes of each door are displayed by the difference of the color of each door.

b) Experimental mouse movement process display: Display the current position of the experimental mouse.

4. Document Management: Generates backup documents by collecting the information obtained.

a) Generate a statistical report: Export the statistics to an Excel table.

b) Generate system log: This system log will include the time and content of each command received and issued by the host computer. (including when the mouse is over, what kind of stimulation, etc.)

When the mouse is moved to the mouse, the mouse pointer changes to a cross line, and the mouse can be dragged to set the initial area.

The experimental system software uses #programming language combined with .NET framework to complete the software writing work, the compilation environment uses Visual Studio 2008. The database uses a MySQL database.

The animal memory training system formed by the above stimulation device is more ideal, and has great significance for research in the fields of neurophysiology, neuropharmacology and psychology.

Claims

WO 2012/119322 Claim PCT/CN2011/071865

1. A closed rotary labyrinth device for an animal memory training system, comprising a circular box and a circuit control system, a plurality of partitions are arranged between the inner wall of the tank and the central cylinder, thereby dividing the box into a plurality of The data collection area and the destination area are provided with the same door opening at the bottom of each partition.

The utility model is characterized in that: the bottom of the box body of the labyrinth device is provided with a turntable driven by a motor, the motor is arranged on the central cylinder, and the output shaft thereof is fastened to the turntable along the axial direction of the central cylinder; The turntable rotates around the central cylinder under the driving of the motor, and a plurality of pulleys supporting the turntable are arranged around a lower portion of the frame of the casing;

a sieve-shaped support net is arranged above the turntable;

A water tank is formed under the turntable to form a manure collection and processing device. A water pipe is arranged along the periphery of the water tank. The water pipe is hooked on the water pipe. The bottom of the water tank is provided with a support frame and a water outlet. With a water brush;

a bracket with a pulley is disposed below the frame of the box;

a liquid crystal screen is disposed on the partition of each section;

The target area is provided with a food trough.

2 . The closed rotary labyrinth device for an animal memory training system according to claim 1 , wherein: two pairs of photoelectric radiation sensors are disposed at each door hole below the partition plate, and the front door is respectively placed behind the door. , output in the form of 'or' to detect the direction of the mouse crossing the door.

3. The closed rotary labyrinth apparatus for an animal memory training system according to claim 1, wherein: said food trough is a drum type with a mesh on the side, and a circular side periphery of said food trough An annular outer casing is provided, the outer casing is divided into two, and the lower mesh can be separately exposed to be exposed by the circuit control system. When the outer casing is opened, the animal eats food from the bottom of the food trough as a training task. The reward, after the power is turned off, the jacket is closed, the grid is sealed, and the animal cannot eat the food.

4. The closed rotary labyrinth apparatus for an animal memory training system according to claim 1, wherein: the number of the partitions is 6, and the circular box is equally angularly divided into A, B, There are six sections C, D, E and F, of which there are 5 data acquisition areas and 1 target area, and 4 door holes are equally spaced under each of the partitions.

5. The closed rotary labyrinth apparatus for an animal memory training system according to claim 1, wherein: the circuit control system comprises a power source, a single chip microcomputer, an eye slap controller, and an automatic flushing drainage system controller;

After the automatic flushing drainage system controller receives the control signal from the single chip microcomputer, the electric valve controller controls the electric valve installed on the fecal collecting and processing device to be turned on or off, so that the feces collecting and processing device is on the water tank The water pipes arranged along the water source are connected or blocked from the water source, and the water tank is periodically flushed, and the sewage flows out from the water outlet of the water tank;

The single chip simultaneously sends a signal to the motor that controls the turntable to realize the rotary motion of the turntable;

The visual squeak controller simultaneously controls the liquid crystal LCD and the scent controller, and after detecting the sinus signal, the scent controller is fresh through the air compressor, the control valve, the odor generator, and the pressure reducing valve The air is delivered to the robotic arm of the training system, which is then transported by the robotic arm to the scent canister located in the labyrinth position.

The closed rotary labyrinth apparatus for an animal memory training system according to claim 1, wherein: the upper portion of the water brush provided in the water tank is fixed at the bottom of the turntable, and the lower brush body and the bottom portion thereof are The side wall of the sink is in contact with the bottom of the trough, and while the turntable rotates, the water brush rotates accordingly, and the lower brush body can wash the water tank.