CN114847183B - Honeycomb type electric shock labyrinth device replacing water labyrinth and video analysis system - Google Patents
Honeycomb type electric shock labyrinth device replacing water labyrinth and video analysis system Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K15/00—Devices for taming animals, e.g. nose-rings or hobbles; Devices for overturning animals in general; Training or exercising equipment; Covering boxes
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K15/00—Devices for taming animals, e.g. nose-rings or hobbles; Devices for overturning animals in general; Training or exercising equipment; Covering boxes
- A01K15/02—Training or exercising equipment, e.g. mazes or labyrinths for animals ; Electric shock devices ; Toys specially adapted for animals
- A01K15/029—Electric or similar shock devices, e.g. prods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
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Abstract
A honeycomb electric shock labyrinth device for replacing a water labyrinth and a video analysis system relate to the technical field of animal experiment devices, and the honeycomb electric shock labyrinth device comprises a labyrinth and a camera; the video analysis system comprises a controller, a computer, an experimental process operation module and a video analysis module, wherein the camera is electrically connected with the controller through a wire, and the controller is electrically connected with the computer through the wire; a plurality of paths are formed in the maze for the mice to pass along the second silk screen and reach the maze unit provided with the inducers. The invention can replace the labyrinth device of Morris water maze, and the experimental process is not dependent on water, thereby effectively widening the application range of the water maze experiment; the invention also reduces the experiment difficulty, saves water sources, can accurately calculate the experiment result data of the exploration track, exploration time, exploration mode, electric shock times and electric shock time of the mice, improves the effectiveness of the experiment result, and reduces the labor capacity of experiment staff.
Description
Technical Field
The invention relates to the technical field of animal experiment devices, in particular to a honeycomb electric shock labyrinth device for replacing a water labyrinth and a video analysis system.
Background
Morris water maze is a classical experiment for studying the learning and memory ability of rodents, and in animal behavioural and neurobiological experiments, water maze is often used to detect the spatial memory ability of animals, which can find the position of a platform in the water maze according to the markers in the environment.
The traditional water maze experimental device and the operation are as follows: the water maze system is divided into a water maze device and a water maze image automatic acquisition and software analysis system. The diameter of the maze is 1.0m and the height is 0.5m. The maze is divided into 4 quadrants, each of which is labeled. The water maze test was divided into two parts and tested continuously for 5 days. The first part is a positioning navigation experiment, which is tested for 4 days, and the second part is a space exploration experiment, which is tested for 1 day. The black (or white) pigment edible toner is put into water to hide the platform, and the black (or white) pigment edible toner is kept in clear contrast with the animal color during image pickup, so that tracking is facilitated. The platform is positioned under the water surface by 0.8cm, the water depth is 21cm, the platform is positioned in the NW quadrant, and the water temperature of the water maze is kept constant (22-24 ℃) by a heating rod. Positioning navigation experiment: each mouse is trained for 4 times per day, and each training interval is 15-20 min. The water inlet point is selected in a semi-random mode each time during the test, and an experimenter holds up the mouse by hand so that the mouse faces the pool wall and lightly puts the mouse into water. In each training, the mice can find a platform within 60s, the residence time on the platform exceeds 2s as a platform seeking success mark, and the time from water entering to platform seeking success of the mice is recorded as a latency period. If the platform was not found in the mice 60s, it was guided to the platform by hand by the experimenter and the latency was noted as 60s. After the mice were on the platform, they were allowed to stay on the platform for 10s to allow spatial learning and memory based on the 4 quadrant reference and to reduce the stress of the mice. Average daily latency values were calculated to assess the ability of the animals to acquire spatial memory. Space exploration experiment: the platform was removed and each mouse tested 1 time for 60s. The SE quadrant was selected to place the mice slightly into the water toward the pool wall edge and the spatial memory of the animals was assessed by the number of passes through the original platform location, the original platform quadrant run ratio and the time ratio (i.e. the ratio of the run time of the original platform quadrant to the total run time) in the animals 60s.
The existing water maze experimental device has the advantages that the water demand is large during experiments, the water temperature needs to be heated to the designated temperature in advance, the water is still required to be kept constant, the water is still required to be changed from the clean angle of the experiments, the temperature of the water is not well controlled, the experimental time is greatly increased, the early preparation of the experiments is more complicated, the working efficiency is reduced, the water consumption is large, and certain waste is caused to water resources. When the animals are taken and placed, a large amount of manpower and material resources are required, experimental errors caused by irregular operation of experimental staff are extremely easy to induce, for example, the water entering mode of the big and small mice should avoid the water entering the nose tip first, and the animals are prevented from choking water.
Not all strains of rats or mice are good at swimming, for example, mice have large gene libraries, high hybridization rate and large individual differences, and are not put on the stage or are guided to enter water again after putting on the stage. The water maze experiment can not be carried out on the big mouse due to the factors such as modeling, for example, the brain stereotactic injection needle retention, the multichannel electrophysiological experiment, the ultramicro imaging experiment and the like, and the water maze experiment can not be carried out any more due to the fact that the head is fixed with the electrode and other elements, so that the detection of the space memory capacity of the big mouse is severely limited.
In order to reduce the labor intensity of experimental staff, improve the experimental efficiency, ensure the accuracy of experimental results and expand the application range of experiments, a labyrinth device capable of replacing Morris water maze is urgently needed.
Disclosure of Invention
The invention provides a honeycomb electric shock labyrinth device for replacing a water labyrinth and a video analysis system, and aims to provide a labyrinth device capable of replacing a Morris water labyrinth, which is not dependent on water any more, and a video analysis system and a use method thereof, so that accurate calculation of track exploration, time exploration, electric shock frequency statistics and electric shock time statistics of a large mouse and a small mouse is realized.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
the honeycomb electric shock labyrinth device comprises a labyrinth and a camera, wherein the camera is arranged above the labyrinth, the labyrinth is formed by connecting a plurality of labyrinth units, adjacent labyrinth units are separated by a first wire netting and a second wire netting which have different shapes, the first wire netting and the second wire netting are connected with each other and enclose the labyrinth units, and the second wire netting can be used for a rat or a mouse to drill; the video analysis system comprises a controller, a computer, an experimental process operation module and a video analysis module, wherein the camera is electrically connected with the controller through a wire, and the controller is electrically connected with the computer through a wire; the first wire netting is electrically connected with the controller through a wire, and can release electric shock stimulus in a safe range when a rat or a mouse touches the first wire netting, the first wire netting and the second wire netting are separated through an insulator, and a plurality of paths which can be used for the rat or the mouse to pass through along the second wire netting and reach a labyrinth unit provided with a primer are formed in the labyrinth.
Preferably, the first wire mesh and the second wire mesh form an electric shock memory reference object, and the difference is only that the shapes of the mesh holes are different.
Preferably, the honeycomb type electric shock labyrinth device further comprises a shading cylinder, a bottom plate is arranged at the bottom of the shading cylinder, a carbon fiber electric heating plate layer is arranged on the inner wall of the shading cylinder, the carbon fiber electric heating plate layer is electrically connected with the controller through a wire, a temperature sensor is further arranged in the shading cylinder, and the temperature sensor is in signal connection with the controller through a wire; the outer wall of the shading cylinder is provided with a plurality of S-shaped vent holes, the top end of the shading cylinder is provided with a cylinder cover, the lower surface of the cylinder cover is provided with a camera and a light supplementing lamp, the light supplementing lamp is electrically connected with a controller through a lead and can adjust brightness under the action of the controller, and the inner surface of the shading cylinder is also provided with a diffuse reflection coating; the central axis of the shading cylinder is used as a reference, and the corresponding inner surfaces of the shading cylinders in the four directions of east, west, south and north are respectively hung with reference objects with different shapes and colors, wherein the reference objects are used for enabling rats or mice to form spatial memory.
Preferably, the labyrinth units are regular hexagons, adjacent labyrinth units are connected with each other through shared sides, each shared side is provided with a first wire netting and a second wire netting, and the first wire netting is electrically connected with the controller through a wire penetrating through the bottom plate.
Preferably, the outside of shading section of thick bamboo still fixedly connected with electric putter, electric putter below be equipped with the control box, control box and shading section of thick bamboo surface fixed connection, electric putter's stiff end and control box top fixed connection, flexible end is along vertically upwards extending to fixedly connected with L shape connecting rod, the horizontal segment tip and the flexible end of L shape connecting rod be connected, the tip and the top fixed connection of bung of vertical section, the control box in be equipped with the controller, electric putter pass through wire and controller electric connection.
Preferably, the experimental process operation module runs through a computer and comprises a temperature detection unit, a timing unit, a video information acquisition unit and a storage unit, and is connected with the video analysis module through the storage unit.
Preferably, the video analysis module comprises a video analysis sub-module and a reference sub-module, and the method for performing video analysis by the video analysis sub-module comprises the following steps: firstly, carrying out smoothing treatment on video information recorded by a storage unit, then counting the movement time, track, electric shock times, total electric shock time and the time for finding food fastest for a rat or a mouse through a software program according to the treated image information, obtaining experimental result data of the track, the time, the mode, the electric shock times and the electric shock time of the rat or the mouse through software analysis, and storing the data in a storage module; the inspection sub-module is provided with an experiment result data inspection window and an experiment process video information inspection window.
Preferably, the camera is a thermal imaging camera, the first wire netting is composed of crisscrossed wires, and the transverse wires above the first wire netting are provided with insulating layers.
The honeycomb electric shock labyrinth device for replacing the water labyrinth has the beneficial effects that: the invention can replace the labyrinth device of Morris water maze, the experimental process is not dependent on water, thus effectively widening the application range of the water maze experiment, and being capable of carrying out maze experiment on various kinds of big and small mice or big and small mice in modeling, leading the research scope of the experiment to be wider and widening the research field; meanwhile, the invention reduces the experiment difficulty, saves water sources, can accurately calculate the experiment result data of the exploration track, exploration time, exploration mode, electric shock times and electric shock time of the rats, improves the effectiveness of the experiment result, and reduces the labor capacity of experiment staff.
Drawings
FIG. 1 is a schematic view of a vertical sectional structure of a shading cylinder of the invention;
FIG. 2 is a schematic view of a partial structure of the labyrinth of the present invention;
FIG. 3 is a schematic diagram of the connection structure of the first wire netting and the second wire netting of the present invention;
FIG. 4 is a schematic diagram of the front structure of the shading barrel of the present invention;
FIG. 5 is a schematic cross-sectional view of a light shielding barrel of the present invention;
1. a bottom plate; 2. a light shielding cylinder; 3. a maze; 31. a first wire netting; 32. a second wire netting; 33. a labyrinth unit; 34. an insulator; 4. a vent hole; 5. a mouse; 6. a primer; 7. an electric push rod; 8. an L-shaped connecting rod; 9. a control box; 10. a barrel cover; 11. a camera; 12. a light supplementing lamp; 13. carbon fiber electric heating plate layer.
Detailed Description
The following detailed description of the embodiments of the present invention in a stepwise manner is provided merely as a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, but any modifications, equivalents, improvements, etc. within the spirit and principles of the present invention should be included in the scope of the present invention.
In the description of the present invention, it should be noted that, the positional or positional relationship indicated by the terms "upper", "lower", "left", "right", "top", "bottom", "inner", "outer", etc. are based on the positional or positional relationship shown in the drawings, are merely for describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, and specific orientation configuration and operation, and thus should not be construed as limiting the present invention.
In the initial embodiment, a honeycomb electric shock maze device and a video analysis system for replacing a water maze can be provided with 2 sets of devices respectively for experiments of rats or mice according to the body type and the size differences of the rats and the mice. As shown in fig. 1-5, the honeycomb type electric shock labyrinth device comprises a labyrinth 3 and a camera 11, wherein the camera 11 is arranged above the labyrinth 3; the maze 3 does not depend on water, so that the problems that the mice are not good at swimming, such as mice with large gene libraries, high hybridization rate and large individual difference, the mice cannot go on the bench or enter water again after being guided to go on the bench, the water maze experiment cannot be carried out by the mice due to the factors such as modeling, and the like are solved, and the maze experiment can be carried out by various mice.
As shown in fig. 1 and 2, the maze 3 is formed by connecting a plurality of maze units 33, the adjacent maze units 33 are separated by a first wire netting 31 and a second wire netting 32 with different shapes, the first wire netting and the second wire netting are connected with each other and enclose the maze units 33, and the second wire netting 32 can be used for a mouse to drill; the video analysis system comprises a controller, a computer, an experimental process operation module and a video analysis module, wherein the camera is electrically connected with the controller through a wire, and the controller is electrically connected with the computer through a wire; the first wire netting 31 is electrically connected with the controller through a wire, and can release electric shock stimulus in a safe range when a mouse touches the first wire netting, the first wire netting and the second wire netting are separated through an insulator 34, and a plurality of paths for the mouse to pass through along the second wire netting and reach a labyrinth unit 33 provided with a primer 6 are formed in the labyrinth 3. When the electric appliance is used, each electric appliance element is connected with a power supply, and the controller enables the electric stimulation to be located in a safe range by adjusting the current of the first wire gauze. When the mouse touches the first wire netting, the mouse can be electrically stimulated, the touch second wire netting can not be electrically shocked, through repeated training and spatial memory, the mouse is learned and only reaches the position of the primer 6 through the second wire netting, and the experimenter can judge the memory degree of the mouse to the first wire netting according to the frequency that the mouse is electrically stimulated. Meanwhile, the mouse can find the primer 6 through the path, and whether the mouse passes the alternative water maze experiment can be judged according to the time of the mouse finding the primer 6. The experimental method and the evaluation index can refer to the specific setting of the water maze experiment.
In a further embodiment, as shown in fig. 3, the first and second wires constitute a shock memory reference, differing only in the shape of the mesh. The mice distinguish the paths to the primer according to the difference of the mesh shapes, and when the mice have the capability, the mice can show the change of the probing mode; and also in the shortening of the probing time.
In a further embodiment, as shown in fig. 4 and 5, the cellular electric shock maze device further includes a light shielding barrel 2, a bottom plate 1 is disposed at the bottom of the light shielding barrel 2, a carbon fiber electric heating plate layer 13 is disposed on the inner wall of the light shielding barrel 2, the carbon fiber electric heating plate layer 13 is electrically connected with the controller through a wire, and a temperature sensor (not shown in the drawing) is further disposed in the light shielding barrel 2, and the temperature sensor is in signal connection with the controller through a wire; the outer wall of the shading cylinder is provided with a plurality of S-shaped vent holes 4, the top end of the shading cylinder is provided with a cylinder cover 10, the lower surface of the cylinder cover 10 is provided with a camera 11 and a light supplementing lamp 12, the light supplementing lamp 12 is electrically connected with a controller through a wire and can adjust brightness under the action of the controller, and the inner surface of the shading cylinder 2 is also provided with a diffuse reflection coating; the central axis of the shading cylinder is used as a reference, and the corresponding four shading cylinder inner surfaces in the north-south direction and the east-west direction are respectively hung with reference objects with different shapes and colors, wherein the reference objects are used for enabling rats or mice to form spatial memory, for example, the shapes and the colors of the 4 reference objects can be as follows: red square, blue circle, green triangle, and yellow crescent. In this embodiment, through setting up temperature sensor and carbon fiber electric heat sheet layer, can carry out thermostatic control in the shading section of thick bamboo 2 to make the mouse participate in the experiment in stable constant temperature environment, avoid the error that environmental factor leads to. Meanwhile, the diffuse reflection coating and the light supplementing lamp are arranged, the brightness in the shading barrel can be adjusted according to the requirement, so that a mouse can perform experiments in a safe environment, and the effectiveness of experimental results can be further ensured. The S-shaped vent holes 4 can ventilate, meanwhile, light leakage of the shading cylinder is avoided, and light uniformity in the shading cylinder is ensured, so that video information collection is facilitated.
In a further embodiment, as shown in fig. 1 and 2, the labyrinth units 33 are regular hexagons, adjacent labyrinth units are connected to each other by shared sides, each shared side is provided with a first wire mesh 31 and a second wire mesh 32, and the first wire mesh 31 is electrically connected to the controller by a wire penetrating through the bottom plate 1.
In a further embodiment, as shown in fig. 4 and 5, the outer side of the shading cylinder 2 is fixedly connected with an electric push rod 7, a control box is arranged below the electric push rod 7, the control box is fixedly connected with the outer surface of the shading cylinder, a fixed end of the electric push rod 7 is fixedly connected with the top end of the control box, a telescopic end extends upwards longitudinally and is fixedly connected with an L-shaped connecting rod 8, the end of a horizontal section of the L-shaped connecting rod 8 is connected with the telescopic end, the end of a vertical section is fixedly connected with the top end of the barrel cover 10, a controller is arranged in the control box, and the electric push rod 7 is electrically connected with the controller through a wire. Under the control of the controller, the barrel cover can be opened and closed at regular time, the stability of the experimental environment is ensured when the barrel cover is closed, and the mouse can be replaced when the barrel cover is opened.
In a further embodiment, the experimental process operation module runs through a computer and comprises a temperature detection unit, a timing unit, a video information acquisition unit and a storage unit, and the experimental process operation module is connected with the video analysis module through the storage unit. When an experiment starts, the temperature detection unit can detect whether the temperature in the shading cylinder meets the experiment standard; the experimenter puts the mouse into a appointed maze unit in the shading section of thick bamboo, puts the primer (usually mouse grain) of lure in another maze unit, starts the timing unit, and electric putter contracts, and the bung is covered, and the camera begins to gather video information simultaneously, and relevant video information can automatic storage memory cell. In the process, the temperature sensor sends a signal to the controller, and the controller regulates and controls the temperature in the shading barrel to be kept constant by regulating the power of the carbon fiber electric heating plate. Meanwhile, according to the definition of the video acquisition information, the brightness of the light supplementing lamp can be automatically adjusted to ensure the effectiveness of the acquisition result, after the experiment reaches the designated time, the barrel cover is opened, the video acquisition is finished, and the experimenter takes out the mice.
In a further embodiment, the video analysis module includes a video analysis sub-module and a review sub-module, and the method for performing video analysis by the video analysis sub-module includes the following steps: firstly, carrying out smoothing treatment on video information recorded by a storage unit, then counting the movement time, track, electric shock times, total electric shock time and the time for finding food fastest for the mouse through a software program according to the treated image information, obtaining experimental result data of the track, the time, the mode, the electric shock times and the electric shock time of the mouse through software analysis, and storing the data in a storage module; the inspection sub-module is provided with an experiment result data inspection window and an experiment process video information inspection window.
In a further embodiment, the camera is a thermal imaging camera, and the first wire mesh is formed by criss-cross wires, wherein the transverse wires above are provided with insulating layers. Because shooting in-process can probably lead to first wire netting and second wire netting to be difficult to distinguish, so set up the horizontal iron wire that is located the top and be equipped with the insulating layer, the setting through the insulating layer can make the horizontal iron wire of top heat up to the certain temperature in the safety zone, can clearly discern first wire netting and second wire netting through thermal imaging, and further clearly discern the key experimental result data such as the exploration orbit of mouse, exploration time, exploration mode, electric shock number of times, electric shock time according to the thermal imaging of mouse itself, further improved the validity and the accuracy of experimental result.
Claims (4)
1. A replace water maze's honeycomb electric shock maze device and video analysis system, characterized by: the honeycomb type electric shock labyrinth device comprises a labyrinth and a camera, wherein the camera is arranged above the labyrinth, the labyrinth is formed by connecting a plurality of labyrinth units, adjacent labyrinth units are separated by a first wire netting and a second wire netting with different shapes, the first wire netting and the second wire netting are connected with each other and enclose the labyrinth units, and the second wire netting can be used for rats or mice to drill; the video analysis system comprises a controller, a computer, an experimental process operation module and a video analysis module, wherein the camera is electrically connected with the controller through a wire, and the controller is electrically connected with the computer through a wire; the first wire netting is electrically connected with the controller through a wire, and can release electric shock stimulus in a safe range when a rat or a mouse touches the first wire netting, the first wire netting and the second wire netting are separated by an insulator, and a plurality of paths which can be used for the rat or the mouse to pass along the second wire netting and reach a labyrinth unit provided with a primer are formed in the labyrinth;
the first wire netting and the second wire netting form an electric shock memory reference object, and the difference is only that the shapes of the meshes are different;
the honeycomb type electric shock labyrinth device also comprises a shading cylinder, a bottom plate is arranged at the bottom of the shading cylinder, a carbon fiber electric heating plate layer is arranged on the inner wall of the shading cylinder, the carbon fiber electric heating plate layer is electrically connected with a controller through a wire, a temperature sensor is further arranged in the shading cylinder, and the temperature sensor is in signal connection with the controller through a wire; the outer wall of the shading cylinder is provided with a plurality of S-shaped vent holes, the top end of the shading cylinder is provided with a cylinder cover, the lower surface of the cylinder cover is provided with a camera and a light supplementing lamp, the light supplementing lamp is electrically connected with a controller through a lead and can adjust brightness under the action of the controller, and the inner surface of the shading cylinder is also provided with a diffuse reflection coating; taking the central axis of the shading cylinder as a reference, and respectively hanging reference objects with different shapes and colors on the inner surfaces of the shading cylinders in the four directions of east, west, south and north, wherein the reference objects are used for enabling rats or mice to form spatial memory;
the labyrinth units are regular hexagons, adjacent labyrinth units are connected with each other through shared sides, each shared side is provided with a first wire netting and a second wire netting, and the first wire netting is electrically connected with the controller through a wire penetrating through the bottom plate;
the camera is a thermal imaging camera, the first wire netting is composed of criss-cross wires, and the transverse wires above the first wire netting are provided with insulating layers.
2. A cellular shock maze device and video analysis system replacing a water maze as defined in claim 1, wherein: the outside of shading section of thick bamboo still fixedly connected with electric putter, electric putter below be equipped with the control box, control box and shading section of thick bamboo surface fixed connection, electric putter's stiff end and control box top fixed connection, flexible end is along vertically upwards extending to fixedly connected with L shape connecting rod, the horizontal segment tip and the flexible end of L shape connecting rod be connected, the tip and the top fixed connection of bung of vertical section, the control box in be equipped with the controller, electric putter pass through wire and controller electric connection.
3. A cellular shock maze device and video analysis system replacing a water maze as defined in claim 2, wherein: the experimental process operation module is operated by a computer and comprises a temperature detection unit, a timing unit, a video information acquisition unit and a storage unit, and is connected with the video analysis module by the storage unit.
4. A cellular shock maze device and video analysis system replacing the water maze as defined in claim 3, wherein: the video analysis module comprises a video analysis sub-module and a reference sub-module, and the video analysis method by the video analysis sub-module comprises the following steps: firstly, carrying out smoothing treatment on video information recorded by a storage unit, then counting the movement time, track, electric shock times, total electric shock time and the time for finding food fastest for a rat or a mouse through a software program according to the treated image information, obtaining experimental result data of the track, the time, the mode, the electric shock times and the electric shock time of the rat or the mouse through software analysis, and storing the data in a storage module; the inspection sub-module is provided with an experiment result data inspection window and an experiment process video information inspection window.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1817326A (en) * | 2006-02-21 | 2006-08-16 | 沈阳医学院 | Mouse space memory retention model and intelligent action inspecting system |
| CN101142900A (en) * | 2007-10-26 | 2008-03-19 | 天津医科大学总医院 | Method and device for reinforcing spatial memory of water maze laboratory animal |
| CN102144572A (en) * | 2011-03-07 | 2011-08-10 | 沈阳医学院 | Closed rotary maze device for animal memory training system |
| CN110506660A (en) * | 2019-08-23 | 2019-11-29 | 宁波大学 | A kind of cross water maze of performance testing |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005529580A (en) * | 2001-08-06 | 2005-10-06 | サイコジェニクス インク | Programmable electronic maze for use in animal behavior assessment |
| CN100477970C (en) * | 2006-02-21 | 2009-04-15 | 沈阳医学院 | Mouse path exercising model and intelligent action inspecting system |
| CN102687676B (en) * | 2012-01-14 | 2013-07-31 | 河南科技大学 | Labyrinth based on color identifying information and animal experiment method |
| FR3039056B1 (en) * | 2015-07-24 | 2017-07-21 | Univ Montpellier | INSTRUMENTALIZED DEVICE FOR CHARACTERIZING THE CAPACITY TO MOVE A MOUSE. |
| JP7137179B2 (en) * | 2017-12-27 | 2022-09-14 | 学校法人同志社 | Maze Assembly Kit for Small Animal Experiments |
| CN110583511B (en) * | 2018-06-12 | 2023-06-13 | 中国科学院昆明动物研究所 | Kiwi three-dimensional maze |
-
2022
- 2022-05-12 CN CN202210516938.3A patent/CN114847183B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1817326A (en) * | 2006-02-21 | 2006-08-16 | 沈阳医学院 | Mouse space memory retention model and intelligent action inspecting system |
| CN101142900A (en) * | 2007-10-26 | 2008-03-19 | 天津医科大学总医院 | Method and device for reinforcing spatial memory of water maze laboratory animal |
| CN102144572A (en) * | 2011-03-07 | 2011-08-10 | 沈阳医学院 | Closed rotary maze device for animal memory training system |
| CN110506660A (en) * | 2019-08-23 | 2019-11-29 | 宁波大学 | A kind of cross water maze of performance testing |
Non-Patent Citations (1)
| Title |
|---|
| 基于小鼠Morris水迷宫和Y迷宫联合试验的多任务行为学测试研究;孔林林;张军;乔枫;祁金顺;;神经解剖学杂志(04);365-368 * |
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