CN102144572B - Closed rotary maze device for animal memory training system - Google Patents
Closed rotary maze device for animal memory training system Download PDFInfo
- Publication number
- CN102144572B CN102144572B CN 201110052637 CN201110052637A CN102144572B CN 102144572 B CN102144572 B CN 102144572B CN 201110052637 CN201110052637 CN 201110052637 CN 201110052637 A CN201110052637 A CN 201110052637A CN 102144572 B CN102144572 B CN 102144572B
- Authority
- CN
- China
- Prior art keywords
- maze
- animal
- rotating disk
- tank
- motor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K15/00—Devices for taming animals, e.g. nose-rings or hobbles; Devices for overturning animals in general; Training or exercising equipment; Covering boxes
- A01K15/02—Training or exercising equipment, e.g. mazes or labyrinths for animals ; Electric shock devices ; Toys specially adapted for animals
Abstract
The invention discloses a closed rotary maze device for an animal memory training system. The maze device comprises a round box body and a circuit control system. Key technical points of the maze device are that: the bottom of the box body of the maze device is provided with a turntable driven by a motor; the motor is arranged on a central cylinder, and an output shaft of the motor is in fastened with the turntable along the axial direction of the central cylinder; the turntable rotates around the central cylinder under the driving of the motor; the periphery of the lower part of a frame of the box body is provided with a plurality of pulleys for supporting the turntable; and a sieve-shaped supporting net is arranged above the turntable. The maze device overcomes the defect of the lateralization in the detection of the conventional maze devices such as a Y-maze and a T-maze. In addition, a device driven by gradient voltage is removed; the maze device effectively prevents animals from finishing experiments by means of smell, and respective disadvantages of a Morris water maze, the Y-maze and a drought maze are weighted; the starlight detection can be performed through program control according to the nocturnal habits of the experimental animals; and the maze device can ensure that the experimental animals are subjected to sensory stimulation in real environment, which is more favorable for acquisition of experimental data.
Description
Technical field
The present invention relates to a kind of model, particularly a kind of closed rotary maze device for animal memory training system of setting up animal learning and memory behavior training method about research.Can be applicable to the fields such as neuro-physiology, Neuropharmacology and psychology.
Background technology
At present, the animal behavior experiment is the indispensable important means of research higher brain function and other Neuscience, especially has more importantly position aspect the behavioral medicine researchs such as neuro-physiology, Neuropharmacology.Wherein maze experiment is the classical project of behavioral experiment, is one of the most frequently used experiment of research rat (or mouse) learning and memory function, also is cognitive drug screening animal model commonly used.
In the task of labyrinth, the instrument of maze device itself can inform that animal needs them how to do (instruction that is similar to human experimentation).In this task, the researcher generally can mention these two concepts of working memory and reference memory (Reference Memory).Animal will be finished a subtask, what at first need is reference memory, it has reflected that animal is to the study of relevant information between each examination time (Trial) in the task (such as the appearance in spatial cues position, labyrinth and experimental rules etc.), therefore animal should be present in each experimentation with reference to information in the process that forms reference memory.In the situation of laboratory, the working memory of animal refers to keep between the examination different in every wheel the (Session) experiment time the ability of information.Comparatively speaking, working memory is different between each examination is inferior, is the memory capability that animal keeps each examination time information (place of just being).After an examination was inferior, rat can wipe current specific working memory, but keeps reference memory constant.Therefore working memory only works in an examination is inferior, and reference memory then belongs to long-term memory.
Existing maze device mainly contains following several: 1, the animal working memory model is measured in non-irrigated labyrinth: non-irrigated labyrinth mainly refers to various radiation traps (radial arm maze), Y labyrinth, T labyrinth etc.Y, T-shaped labyrinth and Barnes maze experiment do not provide bonus penalty terms, the nature of utilizing animal to explore fully, this class labyrinth task is learning and memory under the hungry motivation of animal drives, therefore test the food intake that strictly to control animal in the last week, its weight is alleviated to original about 85%.But the problem that the method exists is exactly to affect experimental result being existed around non-study factors such as arm walking nature and hunger intensities of animal.
2, water maze is measured the animal working memory model: classical water maze model is Morris water maze (Morris water maze, MWM), utilizes animal to avoid water logging and the escape motivation of seeking platform is carried out the learning and memory task.Adding entry in the experimental provision on the basis in non-irrigated labyrinth in addition in the past, forming 8 arm radiation water mazes or T water maze, Y water maze etc., to eliminate the smell clue to the impact of experiment grade.When utilizing water maze to carry out performance testing, do not need to control animal diet followed drinking-water; Required time of animal training is shorter, approximately 1 week; Can utilize calculator to set up image and automatically gather and analytical system, this just can make corresponding histogram and running orbit figure according to the data that gather, and is convenient to the researcher experimental result is made further analysisanddiscusion etc.But the shortcoming of MWM is that latent period and its motion function state of animal swimming are closely related, so affect the normal result that the state of the medicine of animal movement function and animal itself all might affect experiment.In addition, owing to needing surveillance and analysis software, only have minority to equip preferably the laboratory and can carry out the work.The many factors that the design of experimental arrangement need to be considered, need simultaneously the experimenter to possess the knowledge of certain neuro-physiology, cognitive physiology and mathematical statistics aspect, bring difficulty for the explanation of carrying out with the result of experiment, also limited deeply using widely of this labyrinth simultaneously; Because physical demands is too large, body temperature is also lost too much, and old pindone becomes difficulty of task; Be not that all mouse strains all are suitable for the test of Morris labyrinth, (achievement does not improve with the increase of fate, and the school grade of 129/SvJ is deviation also can not to learn this task such as BALB/c; Achievement difference between individuality is huge; Some strain, will encounter difficulties when finishing the learning and memory task take vision as the basis when aging because the has age correlation is looked the road pathology such as the mouse of 129/SvJ strain.In the C57BL/6 strain, because serious alopecia exists, some mouse is produced depressed, add the ulcer dermatitis, finally may affect the Burden-Swimming Ability of KM of mouse and affect experiment grade; To going down of slight ability of learning and memory, this labyrinth program may be insensitive; Animal is immersed in the water causes internal secretion or other stress effect.The interaction of the latter and cerebral lesion or pharmacology operation room has uncertainty; Still need to just can finish by means of manual through the operation of automatic monitor system, this experiment is become some are dull; Shared experimental site is excessive.
3, other mouse behavior models: disposable passive avoidance and diving tower (or pole-climbing) model is the model of two kinds of better simply research learning memories, is applicable to research memory maintenance and memory and consolidates.These two models all are how long the record animal does not enter to the cell of electric shock after suffering once to shock by electricity or do not jump off platform and test the maintenance of memory and consolidation; New object identification experiment is a kind of model of measuring animal non-space working memory ability, has utilized the congenital principle that new object is had the tendency explored of animal.Within the testing period, if animal has been remembered the past heritage body, then animal will be less than new object to the exploration time of past heritage body.
To sum up, the maze device of above-mentioned classics is the limitation of various degrees still, and human factor is more, and technical operation is loaded down with trivial details, lacks the combined influence factor of the conditional stimulus such as electricity, sound (noise) and light etc.For addressing the above problem, the applicant is through constantly research, the technical scheme of number of patent application for " CN200610045868.9 " disclosed: the sense of hearing of a kind of mouse, vision memory model and intelligent behavior detection system, the sense of hearing of this mouse, vision memory model comprises with data collection zone, the labyrinth of purpose district and interface channel thereof, the box labyrinth of circular hidden of multichannel closed-loop path is adopted in this labyrinth, in the casing at the bottom of it is with loam cake and fence, by dividing plate cabinets cavity is separated at least three test sections, one of them is the purpose district, the remainder data acquisition zone, each dividing plate bottom is the identical hole shape passage with adjustable unilateral gate of distribution outward appearance equidistantly, each hole shape access door side has two groups of infrared emission, receive photoelectric cell, in segregate each district the controllable lamp optical illumination is set respectively, the electric shock conductor of the different gradient voltage bands of generation of STOCHASTIC CONTROL is set respectively at the bottom of the fence in each district, in the control cabinet of casing middle standing pillar top, the high light generating means is set, noise generating means and video tracking head.This sense of hearing, vision memory model utilize the controllable lamp optical illumination to adapt to the dark habit that becomes of mouse, make it in casing, carry out autokinetic movement, different gradient voltage bands in conjunction with the electric shock conductor produces stimulate the passive movement that drives the STOCHASTIC CONTROL mouse with electric shock, and fasting and non-fasting mouse all are suitable for.Force the training of animal swimming different from existing water maze, adopt autokinetic movement and the gradient voltage passive matrix of mouse nature activity pattern to combine, prevent that tested mouse from accepting after the single electro photoluminescence away from dedicated tunnel, avoid animal pattern respectively to distinguish the time of staying at data acquisition unit inconsistent, make time class data more accurate.The infrared emission of each passage reveal of labyrinth, reception photoelectric cell can detect the whereabouts of animal in the labyrinth in real time, exactly.Yet the applicant finds this labyrinth model and still has the following disadvantages: shocking by electricity to stimulate drives not gentleness of its effect, and the passive matrix of gradient voltage is easily to laboratory animal generation infringement in various degree, and setting is comparatively complicated; In addition, install perfectly not, laboratory animal may rely on smell finish experiment, and above-mentioned shortcoming all can affect experimental result, affects the accuracy of experimental data.
In sum, according to the actual needs of setting up the model of animal learning and memory behavior training method, existing maze device still need improve.
Summary of the invention
The objective of the invention is for the maze device of the more rational mouse memory training system of a kind of design is provided, overcome the deficiency of the inclined to one side lateral dominance in existing maze device such as Y-maze, the T-maze detection.In addition, removed the setting that gradient voltage drives; And prevent that effectively animal from relying on smell to finish experiment, weighting Morris water maze and Y-maze, non-irrigated labyrinth strong point separately; Can detect according to the program control starlight that carries out of laboratory animal noctural habit; Can make laboratory animal that the sensory stimuli of the true environment placed oneself in the midst of is arranged, be more conducive to measuring of experimental data.
Technical scheme of the present invention is: a kind of closed rotary maze device for animal memory training system, comprise round box and circuit control system, some dividing plates are set between cabinet wall and the center column, thereby described casing is separated into some data collection zone, the purpose district, each dividing plate bottom arranges the identical door opening of outward appearance, its technical essential is: the bottom half setting of described maze device is by motor-driven rotating disk, described motor is arranged on the center column, and its output shaft axially is fastenedly connected with described rotating disk along center column, then described rotating disk rotates around described center column under described driven by motor, and described casing frame lower periphery is provided with the pulley of the described rotating disk of some supports; Described rotating disk top is provided with sieve shape supporting network; Described rotating disk below is provided with tank and forms the collecting dung treating apparatus, along periphery water pipe is arranged on the described tank, evenly offers mouth spray on the described water pipe, and described bottom of gullet is provided with bracing frame and the lower mouth of a river, is provided with the water brush in the described tank; Described casing frame below is provided with the support of pulley; The described dividing plate in each interval is provided with liquid crystal display; Described purpose district is provided with food groove.
Each door opening place of described dividing plate below arranges two pairs of photoelectricity correlation sensors, places respectively behind the door in front of the door, with the formal output of ' or ', thereby detects the direction that mouse is moved into one's husband's household upon marriage.
Described food groove is that the side is with the drum type of grid, the round sides of described food groove is arranged with ring-shaped jacket outward, described overcoat is divided into two, and can separately expose grid down, by described circuit control system control, when overcoat is opened, animal is namely had food as the award of finishing training mission from the grid below of described food groove, and after power supply disconnected, described overcoat was closed, seal described grid, animal namely can't be had food.
Described dividing plate number is 6, and described round box equal angles is separated into A, B, C, D, E, six intervals of F, and wherein 4 door openings that equidistantly distribute are distinguished in 5 of data collection zone, 1 in purpose district below each described dividing plate.
Circuit control system comprises power supply, single-chip microcomputer, looks the sense of smell controller, auto-flushing drainage system controller; After described auto-flushing drainage system controller receives described single-chip microcomputer and sends control signal, the motor-driven valve that its electric valve controller control is installed on the described collecting dung treating apparatus opens or cuts out, make on the tank of described collecting dung treating apparatus along the water pipe of arranging and be communicated with the water source or intercept, regularly wash described tank, sewage flows out from described water channel sewage mouth; Described single-chip microcomputer sends a signal to the motor of the described rotating disk of control simultaneously, realizes rotatablely moving of rotating disk; The described sense of smell controller of looking is controlled liquid crystal display LCD and smell controller simultaneously, described single-chip microcomputer is after detecting scent signal, described smell controller is sent to fresh air by air compressor, control valve, smell generator, pressure-reducing valve the mechanical arm of training system, is delivered to the smell tank that arranges in the position, labyrinth by mechanical arm again.
The described water section of brushing that arranges in the described tank is fixed on described rotating disk bottom, and its underpart brush body contact with trench bottom with described tank sidewall, and in the time of described dial rotation, described water is brushed thereupon rotation, and then the bottom brush body can be scrubbed described tank.
Advantage of the present invention and positive technique effect: the present invention is on the basis of the advantage of fully receiving classical labyrinth, also has following features: 1, be the turntable rotation circular design by exquisiteness, so that every the wheel need be with the tested starting point of putting back between the experiment pilot scale time, can interruptedly not finish the whole experiment of taking turns like this, overcome to a certain extent the deficiency of the inclined to one side lateral dominance of animal in Y-maze, T-maze detect; 2, the auto-flushing drainage system that arranges of labyrinth channel (door opening) can prevent effectively that animal from relying on smell to finish experiment, weighting Morris water maze and Y-maze, non-irrigated labyrinth (Barnes labyrinth) strong point separately; When 3, the present invention utilizes calculator to set up image, automatic data collection and analytical system, realized remote monitoring by means of LAN and Internet according to access rights, detected according to the program control starlight that carries out of laboratory animal noctural habit; 4, visual stimulus unit and smell releasing device synchronous working can make laboratory animal that the sensory stimuli of the true environment placed oneself in the midst of is arranged; Therefore, when the present invention estimates the animal subject ability of learning and memory: adopt mouse autokinetic movement and chassis rotary actuation to be integrated as the motivation of animal subject exploratory response, fasting or non-fasting mouse all are suitable for, meet the reality of animal normal physiological behaviouristics activity; Program control test at night: timing rotation (interval 1-2h) and image, automatic data collection, utilize the nature of mouse nocturnalism and exploration observe to detect, to animal stress be less, therefore can reduce to a certain extent the Confounding Factor of experimental result.Pre-adaptation stage reach: tested animal the raising stage just live in the duplicate labyrinth of detection system outward appearance in, allow it begin to conform from wean, rather than when experiment, just take out and make it be familiar with environment.The equal outstanding behaviours of above characteristics this maze device advantage of laboratory animal being carried out memory training, its experimental data is more accurate, experiment effect is even more ideal.
Description of drawings
The invention will be further described by reference to the accompanying drawings:
Fig. 1 is concrete structure schematic diagram of the present invention;
Fig. 2 is the vertical view of Fig. 1;
Fig. 3 is circuit control block diagram of the present invention;
Fig. 4 is the control block diagram of auto-flushing drainage system of the present invention;
Fig. 5 is the control block diagram of smell releasing device of the present invention;
Fig. 6 is the sink structure schematic diagram of collecting dung treating apparatus of the present invention;
Fig. 7 is the functional block diagram of using mouse memory training system of the present invention;
Sequence number explanation among the figure: 1 casing, 2 dividing plates, 3 liquid crystal displays, 4 motors, 5 sieve shape supporting networks, 6 rotating disks, 7 water pipes, 8 tanks, 9 water brush, 10 support pulleys, 11 supports, 12 pulleys, 13 door openings, 14 food grooves, 15 mouth sprays, 16 times mouths of a river.
Embodiment
Mouse memory training system of the present invention is a kind of device of detection of biological mouse spatial memory capacity, system adopts the box labyrinth of circular hidden as the behavioral data harvester, the closed-loop path that allows experimental mouse form by door opening, finish successively circuit training, utilize the autokinetic movement of mouse and the passive movement of stimulation to record the movement track of mouse, gather this trace information by data acquisition unit.Then be transferred to database in the Control System of Microcomputer by data/address bus, control at any time and monitor the crawler behavior of mouse.
One, couple the present invention does concrete introduction according to Fig. 1-6: the closed rotary maze device that should be used for animal memory training system, comprise round box 1 and circuit control system, some dividing plates 2 are set between casing 1 inwall and the center column, thereby casing is separated into some data collection zone, the purpose district, each dividing plate 2 bottom identical door opening 13 of outward appearance that equidistantly distributes, as shown in Figure 1, the dividing plate number is 6, and the round box equal angles is separated into A, B, C, D, E, six intervals of F, wherein data collection zone is 5,1 in purpose district, each dividing plate 2 below arranges 4 door openings 13.
Wherein: casing 1 bottom is provided with rotating disk 6, center column is provided with motor 4, motor 4 output shafts axially are fastenedly connected with rotating disk 6 centers along center column, and then around the center column rotation, casing frame lower periphery is provided with the pulley 12 of some supporting wheels to rotating disk 6 under motor 4 drives; Rotating disk 6 tops are provided with sieve shape supporting network 5, can be wire netting or plastics net etc.; Rotating disk 6 belows are provided with tank 8 and form the collecting dung treating apparatus, along periphery water pipe 7 are arranged on the tank 8, evenly offer mouth spray 15 on the water pipe 7, and tank 8 bottoms are provided with bracing frame and the lower mouth of a river 16, also are provided with water brush 9 in the tank 8; Casing frame below is provided with the support 11 of pulley 10; Each interval dividing plate 2 is provided with liquid crystal display 3; The purpose district is provided with food groove 14.
The application's circuit control system comprises power supply, single-chip microcomputer, looks the sense of smell controller, auto-flushing drainage system controller etc.Single-chip microcomputer sends a signal to the motor 4 of control rotating disk, realizes rotatablely moving of rotating disk 6.The application's Electric Machine Control adopts solid-state relay control, the power＜100w of drive motors.Solid-state relay adopts 1000w's herein.Single-chip microcomputer is by MOS pipe indirectly control relay.The instruction of Single-chip Controlling motor comes from host computer and sends the instruction of getting off by the CAN bus.CAN and RS232 conversion interface circuit are realized the data transaction between CAN bus protocol and the RS232 agreement.The host computer instruction sends to each child node by this system.
The detection of mouse position: each door opening 13 place that dividing plate below arranges arrange two photoelectricity correlation sensors, place respectively behind the door in front of the door, with the formal output of ' or ', thereby just can detect the direction that mouse is moved into one's husband's household upon marriage, so that the run trace of detection mouse.
Acousto-optic to mouse stimulates: by single-chip microcomputer IO mouth, the grid of control MOS pipe is realized the control to light emitting source (liquid crystal display) and sound generation source, can control the frequency of sounding and luminous intensity.Wherein, single-chip microcomputer connects the data message that main control unit sends as sub-receiving element, extracts command information from data message, and then sends sound stimulation, light stimulus.The control data that can extract stimulation from data message have luminous intensity, audible frequency etc.
The visual stimulus unit of mouse: adopt the ViSaGe visual stimulus generator, belong to prior art.ViSaGe adopts 14bitDCAs image export technique, thereby guarantees the accurate and accurate displaying time of visual stimulus color of image, brightness of ViSaGe control output, even also the frame losing phenomenon can not occur when image switches fast.CRS MATLAB kit is so that adopt MATLAB Programming with Pascal Language output visual stimulus image to become more convenient.Simultaneously, multiple interfaces guarantee ViSaGe can with other device synchronization uses, such as ResonseBox, EyeTracker, EEG, Optotrak and fMRI etc.
Auto-flushing drainage system (dung collection system) controller receives AT89S51 single-chip microcomputer P3.6 (I/O) and sends control signal, control its BFC-2 type electric valve controller work, and then control the electronic motor-driven valve that is installed on the described collecting dung treating apparatus and open/close, make on the tank 8 of collecting dung treating apparatus along the water pipe 7 of arranging and be communicated with the water source or intercept, the running water water source by motor-driven valve to launching gear, be that tank regularly washes, sewage flows out from the described lower mouth of a river.The water of tank 8 interior settings is brushed 9 tops and is fixed on rotating disk 6 bottoms, its underpart brush body contacts with trench bottom with the tank sidewall, in the time of the Single-chip Controlling dial rotation, water brush 9 rotates thereupon, then the bottom brush body can be scrubbed tank, dispose the excreta of realization animal etc., and then eliminate the impact that smell brings experiment effect.
The smell releasing device: single-chip microcomputer is controlled liquid crystal display LCD3 and smell controller simultaneously by looking the sense of smell controller, single-chip microcomputer is after detecting scent signal, by air compressor, control valve, smell generator, pressure-reducing valve fresh air is sent to the mechanical arm that training system arranges, is delivered to the smell tank that arranges in the position, labyrinth by mechanical arm again.
Two, the application's network enabled operation, can realize setting and the operation of remote control system:
Client/server (Client/Server) pattern:Information system under the early stage C/S model is mainly to be that a complete database application system is divided into foreground client computer (front end), application program and three major parts of background server, and by interconnecting between network realization application program and the server, and carry out the transmission of data.The core concept of this structure is exactly that client applications only needs to send request of data to server, and other every operation is all processed by server, and the result that will handle at last turns back in the middle of the application program of client computer.The processing procedure that this moment, server carried out is fully sightless to client applications.Database server and client computer are shared out the work and help one another, the distributed application task of jointly finishing.
When the main feature of two-layer client/server configuration is Processing tasks, to divide the work between the client-server, such advantage is embodied in: when client computer sends request to server, server only need be finished data query task that client computer sends and the result is returned, and so just can reduce the offered load amount of work station.Because the just request of data of client computer of transmission over networks and the result that server returns, it no longer is whole database file, so just can allow the offered load of work station obviously diminish, effectively promote the operating efficiency of the remote client of low speed connection.
Because database adopts the centralized management pattern usually, client computer is by the sql like language accessing database time, and the platform of client computer can be again various, such as PC, Macintosh, UNIX work station etc.; And operating system also can be various, such as Windows, UNIX, 0S/2 etc.Work as like this database structure and change, when perhaps database platform is changed, CLIENT PROGRAM will not need or only just need to carry out the change of minute quantity.We roughly can draw by these characteristics, and database platform and work station are irrelevant, the conclusion that database and client program are irrelevant.Database has adopted the RDBMS based on affairs, keeps transaction journal and database backup/restoration mechanism, so just can carry out well the maintenance of database, in order to guarantee the integrality of database.
The database application exploitation can be divided into three parts on logical meaning: data presentation layer, logic application layer and data service layer.Above-mentioned traditional two-layer client/server configuration can laterally expand, thereby just can obtain three layers or more multi-layered client/server configuration.
Communication protocol:Because the uncertainty of communication process, in order to ensure the error free transmission of data, our final decision is adopted stop-wait protocol and carry out CRC check in communication process.Stop-and-wait protocol: after receiving terminal is correctly received data, return an acknowledgement frame (ACK), transmitting terminal just sends next data.After receiving terminal is received wrong data, return a negative frame (NAK), transmitting terminal retransmits.After loss of data, transmitting terminal can carry out overtime re-transmission.So just guaranteed that transmitting terminal and receiving terminal set up zero defect and be connected.For the correctness of checking data, we adopt the method for CRC check, adopt 8 bit check codes.Therefore our definition frame length is 24,3 bytes.First byte is control bit, and second byte is data bit, and the 3rd byte is the CRC check position.Communicate by letter that we adopt the mode of token bus, master unit scan round subelement between 22 controls node and the main communication nodes.When some subelements when event occurs, this subelement captures bus and master unit is realized point to-point communication.
Three, to the simple description (configuring corresponding stimulating apparatus as example take following concrete maze device) of the training system that utilizes this maze device:
The box shape physical dimension of maze device is diameter 200cm, high 80cm.System space is separated uniformly by 6 dividing plates, evenly distributes 4 in the bottom of every dividing plate and passes through hole (door opening).2 pairs of infrared photoelectric sensors are established at every hole.The bottom is that the iron wire structure of mesh-like can be used to support experimental mouse or the motion thereon of machine mouse, is provided with electric machine rotation at system centre axle place simultaneously, can drive the rotation of screen cloth.Like this, put into the experimental mouse of system or machine mouse and just pass through between the hole back and forth motion under these 6 dividing plates, meanwhile, screen cloth has consisted of the forms of motion of whole system also in ceaselessly rotation.Pass through high 90 mm in hole, wide 110 mm, thick 60 mm, 100 mm that the hole spacing is, the hole dimension of bottom surface screen cloth are 12 x, 12 mm.This system is by the Electric Machine Control dial rotation and then force the experimental mouse training of moving into one's husband's household upon marriage.The function of this training system realizes as shown in Figure 7.
, training system rule set: all to carry out the setting of rule before testing at every turn, rule comprises the prime area of experimental mouse, the attribute of each (representing respectively whether being of passability such as Lv Men and red door), and look experimental mouse and finish training and give single or the combination rewards and punishments.
A) prime area is set: set the prime area of experimental mouse, convenient later statistical work.
B) Hong Men, green door are set: set the attribute of 24 doors, i.e. passability.
C) stimulate kind to set: to stimulate kind to comprise the stimulations such as sound, light, electricity, water, gas, food, image and smell.When experimental mouse during by non-green door (red door), give and different punitive measures, or when experimental mouse successfully pass through green, to and different incentive measure.
Native system can be realized multiple stimulation by mechanical arm, remote control stimulating apparatus.Three kinds of terminal mechanisms that stimulate wherein are set on the mechanical arm, the terminal mechanism that stimulates is the mechanical arm of subsidiary three kinds of pipelines, three kinds of respectively corresponding water, gas, food stimuluss of pipeline, as shown in Figure 8, waterpipe, gas passage, passage of food are set respectively on the mechanical arm, and mechanical arm tail end also is provided with the mobile liquid crystal screen.Stimulating system realizes respectively controlling the supply of water, gas, food by the PLC control system, behind the position that navigates to experimental mouse, the magnetic valve of mechanical arm tail end setting is opened automatically according to the mistake gate signal that PC sends, and discharging accordingly stimulates.
The remote control stimulating apparatus comprises the compositions such as electro photoluminescence module, mechanical stimulus module, sound stimulating module, relay driving module, wireless receiving module and wireless transmitter module, after fitting together, above-mentioned electro photoluminescence, sound stimulation, Mechanical vibration stimulation module, wireless transmitter module, wireless receiving module and relay driving module be fixed on the carrier of laboratory animal health outside binding, this carrier can be resilient canvas, and the canvas two ends are provided with bonding and buckle so that bundle experimental mouse.When experimental mouse detects beginning in the path exercising model of mouse and intelligent behavior monitoring system after, be banded in reception on the experimental mouse back, stimulating apparatus in experimental mouse during by non-green door (red door), the stimulation of setting.
D) stimulation time is set: the stimulation time when at every turn stimulating, also can select after stimulating beginning, manually to stop to stimulate.
E) chassis speed setting: the rotating speed that the chassis is set.
2, data acquisition: whenever carrying out taking turns test all will be to the information of experimental mouse by door.
A) single-wheel route statistics: experimental mouse is from the zone of departure, gets back at last the zone of departure and is defined as one and takes turns.This function statistical experiment mouse walking one take turns through route.
B) overall success and failure number of times statistics: experimental mouse is finished a circle by green door and is defined as successfully, has passed through red door and has been defined as failure, the number of times of this function statistical experiment mouse success and failure.
C) Hong Men, green door are added up by situation: this function statistical experiment mouse is by the number of times of Hong Men and green door.
3, process shows: the position of the attribute of each and experimental mouse can show intuitively in this system.
A) Hong Men, green door show: the difference by each color with each attribute display out.
B) the experimental mouse motion process shows: show the current position of experimental mouse.
4, document management: will add up the Information generation backup document that obtains.
A) generate statistical report form: statistical information is exported in the Excel form.
B) generation system daily record: this system journal will comprise that host computer is subject to and time and the content of each bar order of sending.(comprising when what door when experimental mouse cross, be subject to which type of stimulation etc.)
When mouse moved on to experimental mouse, it is capable that mouse pointer becomes cross, and can drag experimental mouse and set the prime area this moment.
This experimental system software adopts the C# programming language in conjunction with the work of writing of .NET framework software, and translation and compiling environment adopts Visual Studio 2008.Database adopts the MySQL database.
It is more desirable that the present invention configures the animal memory training system experiment effect that above-mentioned stimulating apparatus forms, and the research in the fields such as neuro-physiology, Neuropharmacology and psychology is had unusual meaning.
Claims (6)
1. closed rotary maze device that is used for animal memory training system, comprise round box and circuit control system, some dividing plates are set between cabinet wall and the center column, thereby described casing is separated into some data collection zone and purpose district, each dividing plate bottom arranges the identical door opening of outward appearance
It is characterized in that: the bottom half setting of described maze device is by motor-driven rotating disk, described motor is arranged on the center column, and its output shaft axially is fastenedly connected with described rotating disk along center column, then described rotating disk rotates around described center column under described driven by motor, and described casing frame lower periphery is provided with the pulley of the described rotating disk of some supports;
Described rotating disk top is provided with sieve shape supporting network;
Described rotating disk below is provided with tank and forms the collecting dung treating apparatus, along periphery water pipe is arranged on the described tank, evenly offers mouth spray on the described water pipe, and described bottom of gullet is provided with bracing frame and the lower mouth of a river, is provided with the water brush in the described tank;
Described casing frame below is provided with the support of pulley;
The described dividing plate in each interval is provided with liquid crystal display;
Described purpose district is provided with food groove.
2. the closed rotary maze device for animal memory training system according to claim 1, it is characterized in that: each door opening place of described dividing plate below arranges two pairs of photoelectricity correlation sensors, place respectively behind the door in front of the door, with the formal output of ' or ', thereby detect the direction that mouse is moved into one's husband's household upon marriage.
3. the closed rotary maze device for animal memory training system according to claim 1, it is characterized in that: described food groove is that the side is with the drum type of grid, the round sides of described food groove is arranged with ring-shaped jacket outward, described overcoat is divided into two, under can separately expose grid, by described circuit control system control, when overcoat is opened, animal is namely had food as the award of finishing training mission from the grid below of described food groove, after power supply disconnects, described overcoat is closed, and seals described grid, and animal namely can't be had food.
4. the closed rotary maze device for animal memory training system according to claim 1, it is characterized in that: described dividing plate number is 6, and described round box equal angles is separated into A, B, C, D, E, six intervals of F, wherein 4 door openings that equidistantly distribute are distinguished in 5 of data collection zone, 1 in purpose district below each described dividing plate.
5. the closed rotary maze device for animal memory training system according to claim 1 is characterized in that: circuit control system comprises power supply, single-chip microcomputer, looks the sense of smell controller, auto-flushing drainage system controller;
After described auto-flushing drainage system controller receives described single-chip microcomputer and sends control signal, the motor-driven valve that its electric valve controller control is installed on the described collecting dung treating apparatus opens or cuts out, make on the tank of described collecting dung treating apparatus along the water pipe of arranging and be communicated with the water source or intercept, regularly wash described tank, sewage flows out from described water channel sewage mouth;
Described single-chip microcomputer sends a signal to the motor of the described rotating disk of control simultaneously, realizes rotatablely moving of rotating disk;
The described sense of smell controller of looking is controlled liquid crystal display LCD and smell controller simultaneously, described single-chip microcomputer is after detecting scent signal, described smell controller is sent to fresh air by air compressor, control valve, smell generator, pressure-reducing valve the mechanical arm of training system, is delivered to the smell tank that arranges in the position, labyrinth by mechanical arm again.
6. the closed rotary maze device for animal memory training system according to claim 1, it is characterized in that: the described water section of brushing that arranges in the described tank is fixed on described rotating disk bottom, its underpart brush body contacts with trench bottom with described tank sidewall, in the time of described dial rotation, described water brush rotates thereupon, and then the bottom brush body can be scrubbed described tank.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110052637 CN102144572B (en) | 2011-03-07 | 2011-03-07 | Closed rotary maze device for animal memory training system |
| GB1309005.5A GB2498685B (en) | 2011-03-07 | 2011-03-16 | Closed rotary maze device for animal memory training system |
| PCT/CN2011/071865 WO2012119322A1 (en) | 2011-03-07 | 2011-03-16 | Closed rotary maze device for animal memory training system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110052637 CN102144572B (en) | 2011-03-07 | 2011-03-07 | Closed rotary maze device for animal memory training system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102144572A CN102144572A (en) | 2011-08-10 |
| CN102144572B true CN102144572B (en) | 2013-03-06 |
Family
ID=44419210
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110052637 Expired - Fee Related CN102144572B (en) | 2011-03-07 | 2011-03-07 | Closed rotary maze device for animal memory training system |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN102144572B (en) |
| GB (1) | GB2498685B (en) |
| WO (1) | WO2012119322A1 (en) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102422818A (en) * | 2011-09-01 | 2012-04-25 | 上海移数信息科技有限公司 | High-grade full-automatic intelligent analysis system of Morris water maze |
| CN102577996B (en) * | 2012-03-14 | 2013-08-14 | 浙江大学城市学院 | Noise Y labyrinth device |
| CN102960264B (en) * | 2012-12-17 | 2014-06-18 | 杭州朗拓生物科技有限公司 | Device and method used for testing light favor behavior of poultry |
| CN103070671B (en) * | 2013-01-31 | 2014-07-30 | 郑州大学 | Automatic assessment system for visual cognition behavior function of rodent |
| CN103125406B (en) * | 2013-03-19 | 2015-03-18 | 郑州大学 | Visual cognitive behavioral learning automatic training system of big and small mice |
| FR3039056B1 (en) * | 2015-07-24 | 2017-07-21 | Univ Montpellier | INSTRUMENTALIZED DEVICE FOR CHARACTERIZING THE CAPACITY TO MOVE A MOUSE. |
| CN106614102B (en) * | 2017-01-24 | 2022-07-12 | 中国农业科学院蜜蜂研究所 | Experimental device for simulating influence of automobile transportation on bees |
| CN107787872B (en) * | 2017-12-12 | 2023-05-09 | 沈阳医学院 | Rodent space learning memory training system |
| CN107980656B (en) * | 2017-12-30 | 2023-05-05 | 沈阳医学院 | Learning and memory device for training animal mine clearance |
| CN108207695B (en) * | 2017-12-30 | 2023-08-01 | 沈阳医学院 | Simulated container poison and explosion searching training device for animals |
| CN108925457B (en) * | 2018-06-09 | 2021-03-02 | 过蕊 | Training runway for pet training |
| CN110583511B (en) * | 2018-06-12 | 2023-06-13 | 中国科学院昆明动物研究所 | Kiwi three-dimensional maze |
| CN108739443A (en) * | 2018-07-31 | 2018-11-06 | 中国人民解放军陆军军医大学第附属医院 | A kind of animal illumination experiment case |
| CN111685049B (en) * | 2019-03-12 | 2022-01-21 | 中国科学院脑科学与智能技术卓越创新中心 | Method for space working memory behavior training |
| CN110024750B (en) * | 2019-05-21 | 2024-02-23 | 中国农业科学院蜜蜂研究所 | Box for measuring spatial memory behavior of bees and measuring method thereof |
| CA3156089A1 (en) * | 2019-10-22 | 2021-04-29 | Universite D'aix Marseille (Amu) | Apparatus and system for testing rodents' cognitive ability |
| CN112655587B (en) * | 2020-11-26 | 2023-06-02 | 西安医学院 | Full-automatic intelligent water maze experimental device |
| CN114190298B (en) * | 2021-12-13 | 2022-12-27 | 复旦大学 | Method for detecting spatial and environmental memory capacity of mice under negative emotion |
| CN114208695B (en) * | 2021-12-13 | 2023-04-28 | 复旦大学 | Device for detecting space and environment memory capacity under negative emotion of mouse |
| CN114847183B (en) * | 2022-05-12 | 2023-06-06 | 山东中医药大学 | Honeycomb type electric shock labyrinth device replacing water labyrinth and video analysis system |
| CN114847229B (en) * | 2022-06-02 | 2023-05-26 | 山西医科大学第一医院 | Behavioural method for evaluating learning and memory of mice based on running platform |
| CN115633644B (en) * | 2022-11-15 | 2023-06-20 | 斯贝福（北京）生物技术有限公司 | Excitation type animal movement feeding device |
| CN116267693A (en) * | 2023-05-05 | 2023-06-23 | 东莞市滨海湾中心医院(东莞市太平人民医院、东莞市第五人民医院) | Foraging tester and foraging test method for evaluating learning memory of mice |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1817326A (en) * | 2006-02-21 | 2006-08-16 | 沈阳医学院 | Mouse space memory retention model and intelligent action inspecting system |
| CN201967488U (en) * | 2011-03-07 | 2011-09-14 | 沈阳医学院 | Closed rotary maze device for animal memory training system |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3857364A (en) * | 1973-03-30 | 1974-12-31 | F Miller | Adjustable animal maze device |
| JPS52106936A (en) * | 1976-03-05 | 1977-09-08 | Hitachi Ltd | Device for studying and observing animal maze |
| WO2003013429A2 (en) * | 2001-08-06 | 2003-02-20 | Psychogenics, Inc. | A programmable electronic maze for use in the assessment of animal behaviour |
| CN100471465C (en) * | 2006-02-21 | 2009-03-25 | 沈阳医学院 | Mouse hearing and vision memory model and intelligent action inspecting system |
| CN102119669B (en) * | 2011-03-07 | 2012-11-21 | 沈阳医学院 | Animal memory training system using closed rotary maze |
| CN102160527B (en) * | 2011-03-07 | 2012-11-28 | 沈阳医学院 | Intelligent wireless remote control simulation device used for animal memory training system |
| CN201967487U (en) * | 2011-03-07 | 2011-09-14 | 沈阳医学院 | Intelligent wireless remotely-controlled stimulating device for animal memory training system |
| CN102133908B (en) * | 2011-03-07 | 2012-07-04 | 沈阳医学院 | Bionic rat moving structure for memory training system for animals |
| CN202035374U (en) * | 2011-03-07 | 2011-11-16 | 沈阳医学院 | Automated mechanical arm stimulation device used in animal-memory training system |
| CN102119670B (en) * | 2011-03-07 | 2012-10-03 | 沈阳医学院 | Automated mechanical arm stimulating device for animal memory training system |
| CN202016516U (en) * | 2011-03-07 | 2011-10-26 | 沈阳医学院 | Simulated mouse moving structure used for animal memory training system |
-
2011
- 2011-03-07 CN CN 201110052637 patent/CN102144572B/en not_active Expired - Fee Related
- 2011-03-16 GB GB1309005.5A patent/GB2498685B/en not_active Expired - Fee Related
- 2011-03-16 WO PCT/CN2011/071865 patent/WO2012119322A1/en active Application Filing
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1817326A (en) * | 2006-02-21 | 2006-08-16 | 沈阳医学院 | Mouse space memory retention model and intelligent action inspecting system |
| CN201967488U (en) * | 2011-03-07 | 2011-09-14 | 沈阳医学院 | Closed rotary maze device for animal memory training system |
Also Published As
| Publication number | Publication date |
|---|---|
| GB2498685A (en) | 2013-07-24 |
| CN102144572A (en) | 2011-08-10 |
| WO2012119322A1 (en) | 2012-09-13 |
| GB2498685B (en) | 2015-06-10 |
| GB201309005D0 (en) | 2013-07-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102144572B (en) | Closed rotary maze device for animal memory training system | |
| CN102119669B (en) | Animal memory training system using closed rotary maze | |
| CN201967488U (en) | Closed rotary maze device for animal memory training system | |
| CN100471465C (en) | Mouse hearing and vision memory model and intelligent action inspecting system | |
| CN100471466C (en) | Mouse space memory retention model and intelligent action inspecting system | |
| CN103691058B (en) | The deep brain stimulation FPGA experiment porch of parkinson disease basal nuclei-thalamus network | |
| CN102500058B (en) | Neurologic disease physiotherapeutic instrument based on brain waves and use method thereof | |
| CN104983406B (en) | A kind of network TCM pulse diagnosis system | |
| CN104112066A (en) | Epilepsy state closed-loop control experiment platform based on FPGA | |
| CN100455015C (en) | Integrated vision monitoring multi-mode wireless computer interactive apparatus | |
| Cangelosi et al. | The processing of verbs and nouns in neural networks: Insights from synthetic brain imaging | |
| CN109920551A (en) | Autism children social action performance characteristic analysis system based on machine learning | |
| CN105165661B (en) | For double-deck semi-enclosed automatic " ten " the font labyrinth of pigeon | |
| CN106363644A (en) | Intelligent robot for internet education service | |
| CN100477970C (en) | Mouse path exercising model and intelligent action inspecting system | |
| CN108158803A (en) | A kind of channels and collaterals detect intelligence system and its detection method automatically | |
| CN107102108A (en) | A kind of aquaculture water quality monitoring system | |
| Takamiya et al. | Dynamics of memory engrams | |
| Hu et al. | Rehabilitation of motor function after stroke: a bibliometric analysis of global research from 2004 to 2022 | |
| Jørgensen et al. | Behavioral encoding across timescales by region-specific dopamine dynamics | |
| CN102160527B (en) | Intelligent wireless remote control simulation device used for animal memory training system | |
| Bradshaw | The evolution of intellect: Cognitive, neurological, and primatological aspects and hominid culture | |
| CN206100294U (en) | Medical treatment desensitization system | |
| CN106618723A (en) | Intelligent low-temperature cold plasma medical treatment equipment | |
| Fountas et al. | Phase offset between slow oscillatory cortical inputs influences competition in a model of the basal ganglia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130306 Termination date: 20200307 |