CN114208695A - Device for detecting space and environment memory capacity of mouse under negative emotion - Google Patents
Device for detecting space and environment memory capacity of mouse under negative emotion Download PDFInfo
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Abstract
The invention provides a device for detecting space and environment memory capacity of a mouse under negative emotion, which comprises an animal behavioristics video analysis system, a sound insulation box, a rotary table and a behavior box, wherein the animal behavioristics video analysis system comprises a monitoring analysis background and an industrial camera; the behavior box comprises a bottom plate, three detection box bodies which are fixedly arranged on the bottom plate, have an included angle of 120 degrees and are provided with openings at the upper ends, and middle passageways which are arranged among the lower parts of the three detection box bodies and are respectively connected with the three detection box bodies, wherein each detection box body comprises a box door, a bottom electric shock plate and three replaceable wall plates; the industrial camera is arranged in the sound insulation box or outside the sound insulation box and is positioned above the behavior box. The device can be used for effectively carrying out experimental detection on the space and environment information memory capacity of the mouse under negative emotion and the priority of the mouse for acquiring the environment and the space information, and fills the blank of the experimental detection device.
Description
Technical Field
The invention relates to the technical field of animal space and environment memory detection, in particular to a device for detecting space and environment memory capacity of a mouse under negative emotion.
Background
The ability to discern environmental and azimuthal trends, and avoidance, is very important to the survival of animals. In a number of cognitive science paradigms, the brain of a mouse has been found to be able to process a wide variety of information, particularly when the mouse is injured at a location that the mouse remembers the environment and orientation at that time and avoids later in its activity. However, the information acquisition sequence of the mouse in such memory of environment and orientation and the sensitivity of the mouse to the memory of environment and orientation are not known at present.
Among devices for detecting the mouse spatial cognition ability, the devices such as the Y maze, the T maze, the water maze and the like are commonly applied at present. However, the Y maze, the T maze and the water maze are more in the detection of the spatial memory capability of the mouse, do not contain the information of the environment where the mouse is located, and can not meet the detection of the acquisition priority of the environment and the spatial information of the mouse, and the water maze occupies a large area and is inconvenient to replace the water body in the maze. In a detection device with three boxes presenting a Y shape, which is invented by Rutsuko Ito of Cambridge university in 2006, the detection of the spatial cognition ability of a rat when a reward is obtained in a certain environment can be realized, but the device can only realize the detection of the spatial cognition ability of the rat in positive emotion, and cannot detect the memory of the rat to the environment and the space in negative emotion.
Negative emotions in mice refer to avoidance emotions that occur when mice are exposed to noxious or punitive stimuli. There are many noxious or punitive stimuli that can cause negative emotions in mice, such as delivery of a foot shock, injection of formalin into the foot, tail shock, noise stimulation, injection of lithium chloride causing abdominal discomfort, and the like. The development of negative emotions in mice can generally be detected using conditional location preference experiments. For mice, noxious or punitive stimuli are naturally aversive stimuli, while environmental and spatial information in the housing belong to neutral stimuli. When the mouse receives the negative stimulation, the mouse can jointly learn the negative stimulation and the environmental or spatial information, namely, the negative stimulation is matched with the environmental or spatial information. If the mouse develops a negative emotional memory of environmental and spatial information, the mouse will exhibit evasive behavior in the environment and space that is subject to the negative stimuli when it is returned to the same environment and space again.
The mouse generates avoidance behaviors to environments and spaces which are once injured, is a survival instinct which is favorable for avoiding injury, and has great research value for detecting the survival instinct. However, the experimental device such as the above mentioned one in the prior art can not effectively detect the memory of environmental and spatial information under the negative emotion of the mouse. On the other hand, the mouse has strong spatial position perception capability, and the mouse has various strains, and the technology of optogenetics, electrophysiology and the like is mature, so that the model animal is an ideal model animal for researching the function of avoiding hurting the function. By combining the characteristics, the extraction of spatial and environmental information in the aversion caused by pain or electric shock of the mouse is detected, and the feasibility and the research value are realized. Therefore, there is a need for a device that can detect the ability of a mouse to remember spatial and environmental information when it is injured.
Disclosure of Invention
The purpose of the invention is: aiming at the problems in the prior art, the device for detecting the spatial and environmental memory abilities under the negative emotion of the mouse is provided, and effective experimental detection can be implemented on the spatial and environmental information memory abilities of the mouse when the mouse is injured by the device.
The technical scheme of the invention is as follows: the device for detecting the space and environment memory capacity of the mouse under the negative emotion comprises an animal behavioural video analysis system which comprises a monitoring analysis background and an industrial camera electrically connected with the monitoring analysis background, and is structurally characterized in that: the device also comprises a sound insulation box, a rotary table which is arranged in the sound insulation box and can rotate, and a behavior box which is arranged on the rotary table and is used for accommodating a mouse to carry out a detection test when being used; the behavior box comprises a bottom plate, three detection box bodies which are fixedly arranged on the bottom plate, have an included angle of 120 degrees with each other and are provided with openings at the upper ends, and middle passageways which are fixedly arranged among the lower parts of the three detection box bodies and are respectively connected with the three detection box bodies; the industrial camera is arranged in the sound insulation box or outside the sound insulation box and is positioned above the behavior box.
The further scheme is as follows: the sound insulation box comprises a box body, a sound insulation layer arranged on the inner periphery of the box body, a white light lamp arranged outside the box body or in the box body and used for lighting when being positioned above the behavior box for use, and an infrared lamp used for assisting the industrial camera to shoot; the white light lamp and the infrared lamp are electrically connected with the monitoring analysis background.
The further scheme is as follows: the sound insulation box also comprises a suspension arm which can move up and down to adjust the position in the box body, and the industrial camera, the white light lamp and the infrared lamp are respectively fixed on the suspension arm and positioned above the behavior box.
The further scheme is as follows: the detection box body comprises 4 longitudinal beams, 3 wall plates, 1 box door and 1 electric shock plate; each longitudinal beam is provided with an inserting groove for inserting the wall plate; the lower ends of the 4 longitudinal beams are respectively fixedly connected with the bottom plate, and the two sides of each wall plate are respectively matched and spliced with the splicing grooves of the corresponding 2 longitudinal beams; the box door is arranged at the joint of the detection box body and the middle passageway in an openable and closable manner; the electric shock plates are respectively and fixedly arranged at 1 position on the lower side of the detection box body and are arranged in a suspended mode relative to the upper end of the bottom plate; the electric shock board is electrically connected with the monitoring analysis background.
The further scheme is as follows: the wall boards comprise transparent wall boards, semitransparent wall boards, transparent wall boards covering a half of the wall boards, white wall boards and wall boards with transverse stripes and vertical stripes on the inner sides of the wall boards.
The further scheme is as follows: the behavior box also comprises a top connecting frame which is inserted with the upper ends of the longitudinal beams arranged on the periphery of the bottom plate; the box door is a sliding door.
The further scheme is as follows: the middle passageway comprises 3 connecting elbows, a central support piece and a passageway plate; each connecting elbow is respectively and fixedly connected with the lower parts of a group of 2 adjacent longitudinal beams arranged on the inner side of the bottom plate, and the central support piece and the 3 connecting elbows are respectively and fixedly connected and suspended above the bottom plate; the crossing plate is fixedly arranged on the central supporting piece.
The further scheme is as follows: the aisle plate is an electric shock plate, and the upper end surface of the aisle plate is flush with the upper end surface of the electric shock plate of the detection box body; the channel passing board is electrically connected with the monitoring analysis background.
The further scheme is as follows: the rotary table is a manual rotary table or an electric rotary table; and when the turntable is an electric turntable, the turntable is electrically connected with the monitoring analysis background.
The further scheme is as follows: the detection box body is a square box body.
The invention has the positive effects that: (1) according to the invention, through the respective structures of the behavior box, the turntable, the sound insulation box and the like and the overall structural design including mutual matching, when the device is used for an experiment, effective experiment detection can be carried out on the space and the environment information memory capacity when a mouse is injured and the priority of the mouse for acquiring the environment information and the space information, and the blank of the experiment detection device is filled. (2) According to the invention, the installation mode of the wall board of the behavior box is set to be a slot-inserting type drawable mode, and the wall boards of different materials and patterns are matched, so that the wall boards can be conveniently replaced, combined and matched according to different experiment purposes during experiments, a set of device can implement various experiments, and the cost of experiment equipment is effectively reduced. (3) The bottom plates of the 3 detection box bodies of the behavior box are arranged to be the electric shock plates, so that the behavior box can be conveniently matched with the experiment requirement to enable a mouse to generate fear memory when in use, and the operation is simple and convenient. (4) According to the invention, the behavior box is arranged on the turntable, so that the behavior box can be conveniently rotated according to a set angle, and the interference of spatial information can be eliminated by rotating the behavior box according to the requirements of part of detection experiments, so that the detection result of the experiment is more accurate. (5) According to the invention, the sound insulation box is arranged, and the turntable and the behavior box are arranged in the sound insulation box, so that the interference of an external sound environment on a mouse can be well queued during experimental detection, and the accuracy of the detection experimental result is favorably improved.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic perspective view of the behavior box of FIG. 1;
FIG. 3 is a schematic perspective view of the top connection frame of FIG. 2 with the top connection frame removed;
FIG. 4 is a schematic perspective view of the wall panel of FIG. 3 with the wall panel removed;
FIG. 5 is a schematic view of the structure of FIG. 4 with the stun plate and aisle plate removed;
FIG. 6 is a schematic structural view of the stringer of FIG. 4;
FIG. 7 is a schematic structural view of the electric stun plate of FIG. 4;
figure 8 is a schematic view of the connecting elbow of figure 5.
The reference numbers in the above figures are as follows:
the device comprises a behavior box 1, a bottom plate 11, a detection box body 12, a longitudinal beam 12-1, a support column 12-1-1, a mounting column 12-1-2, a plug-in groove 12-1-3, a wall plate 12-2, a box door 12-3 and an electric shock plate 12-4; mounting side plates 12-4-1, conducting rods 12-1-2, a middle passageway 13, a connecting elbow 13-1, a main connecting part 13-1-1, a connecting part 13-1-2, a central supporting part 13-2, a channel passing plate 13-3 and a top connecting frame 14;
the sound insulation box 2, the box body 21, the sound insulation layer 22, the suspension arm 23, the white light lamp 24 and the infrared lamp 25;
a turntable 3;
the animal behavior video analysis system 4, the monitoring analysis background 41 and the industrial camera 42.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
(example 1)
Referring to fig. 1, the device for detecting the spatial and environmental memory ability under the negative emotion of the mouse of the embodiment mainly comprises a behavior box 1, a sound insulation box 2, a turntable 3 and an animal behavior video analysis system 4.
Referring to fig. 2 to 8, the behavior box 1 mainly comprises a bottom plate 11, 3 detection boxes 12 which are fixedly arranged on the bottom frame plate 11, have an included angle of 120 ° and are provided with openings at upper ends, and have the same structure, a middle aisle 13 which is fixedly arranged between lower portions of the 3 detection boxes 12 and is respectively connected with the 3 detection boxes 12, and a top connection frame 14.
Bottom plate 11 is the hexagonal board spare for the periphery, has 3 long limits and 3 minor faces in 6 outer peripheries of bottom plate 11, and 3 long limits and 3 minor faces are consecutive at interval end to end, and 6 summits of bottom plate 11 are on same circumference. Preferably, the diameter of the circle on which the 6 apexes of the bottom plate 11 lie is 70 cm.
Each detection box body 12 consists of 4 longitudinal beams 12-1, 3 wall plates 12-2, 1 door 12-3 and 1 electric shock plate 12-4. The structure of the longitudinal beam 12-1 is shown in figure 6, the longitudinal beam 12-1 is integrally formed by a support column 12-1-1 and a mounting column 12-1-2, the upper end of the mounting column 12-1-2 is flush with the upper end of the support column 12-1-1, the lower end of the mounting column 12-1-2 is shorter than the lower end of the support column 12-1-1 so as to be convenient for hanging and mounting an electric shock plate 12-4, an inserting groove 12-1-3 for inserting with a wall plate 12-2 is arranged between the support column 12-1-1 and the mounting column 12-1-2, the upper end of the support column 12-1-1 is provided with an inserting hole for inserting with a top connecting frame 14, the mounting column 12-1-2 is provided with a mounting connecting hole (not marked in the figure), and each longitudinal beam 12-1 is fixedly connected with a bottom plate by the lower end of the support column 12-1-1. The two sides of each wall plate 12-2 are respectively matched and spliced with the splicing grooves 12-1-3 of the corresponding 2 longitudinal beams 12-1, and the splicing mode is adopted, so that the wall plates 12-2 can be conveniently replaced according to the requirements of detection experiments when in use. The wall board 12-2 is provided with a transparent wall board, a semitransparent wall board, a transparent wall board for shielding a half of the wall board, a white wall board and wall boards with horizontal stripes and vertical stripes on the inner sides of the wall boards, and the horizontal stripes and the vertical stripes can be arranged on the corresponding wall boards by adopting a mode of sticking paper and the like. Different wall panels 12-2 are selected accordingly according to different requirements of the detection experiment paradigm. The doors 12-3 are each provided with 1 leaf by means of a corresponding longitudinal beam 12-1 at the junction of each detection box body 12 and the middle passage 13, and preferably, the doors 12-3 are sliding doors so as to be convenient to arrange and open and close during use. Structure of electric shock plate 12-4 referring to fig. 7, the electric shock plate 12-4 is composed of 2 parallel mounting side plates 12-4-1 and a plurality of conducting rods 12-1-2 arranged between the 2 mounting side plates 12-4-1, two sides of each mounting side plate 12-4-1 are provided with connecting lugs protruding upwards, the electric shock plate 12-4 is correspondingly matched and fixedly connected with the lower ends of the mounting columns 12-1-2 of the 4 longitudinal beams 12-1 by the connecting lugs on the 2 mounting side plates 12-4-1, so that the electric shock plate 12-4 is suspended above the bottom plate 11. The electric shock plate 12-4 is used for carrying out short-time electric shock on the soles of the mice according to experimental needs so as to bring the mice with the fear/aversion memory of detecting the environment in the box body 12. Preferably, the 3 detection chambers 12 are square chambers.
The middle passageway 13 mainly comprises 3 connecting elbows 13-1, a central support piece 13-2 and a passageway plate 13-3; the structure of the connecting elbow 13-1 is shown in fig. 8, the connecting elbow 13-1 is integrally composed of an overall arc-shaped main connecting part 13-1-1 and a strip-shaped connecting part 13-1-2, each connecting elbow 13-1 is fixedly connected with the lower parts of a group of 2 adjacent longitudinal beams 12-2 arranged on the inner side of the bottom plate back to back through the main connecting part 13-1-1, and referring to fig. 5, the central supporting part 13-2 is fixedly connected with the connecting part 13-1-2 of each connecting elbow 13-1 so as to be suspended above the bottom plate 11. The aisle plates 13-3 are fixedly arranged on the central support piece 13-2, and the upper end surfaces of the aisle plates 13-3 are flush with the upper end surfaces of the electric shock plates 12-4 of the detection box bodies 12. Preferably, the aisle boards 13-3 are also shock boards, so that the transient shock is discharged by the aisle boards 13-3 to drive the mice into the box when the mice linger in the middle aisle 13 and do not enter the corresponding detection box 12 in the detection experiment process.
The top connection frame 14 is preferably arranged, the top connection frame 14 is a narrow strip-shaped plate member which is in a Y shape as a whole, a plug connector which is matched with the plug hole at the upper end of the support column 12-1-1 of the longitudinal beam 12-1 is arranged on the lower end surface of the top connection frame 14, and the plug connector on the lower end surface of the top connection frame 14 is plugged with the plug hole at the upper end of the support column 12-1-1 of the 6 longitudinal beams 12-1 which are arranged on the periphery of the bottom plate 11. The top attachment frame 14 serves to further reinforce the inspection boxes 12 and provide an aesthetically pleasing appearance.
Still referring to fig. 1, the soundproof case 2 is mainly composed of a case body 21, a soundproof layer 22, a suspension arm 23, a white light lamp 24, and an infrared lamp 25. The box body 21 is a square box body with an opening at the upper end, and the upper side of the box body 21 is provided with an opening for placing the suitcase 1; the sound insulation layer 22 is arranged around the box body 21; the suspension arm 23 is preferably disposed, the suspension arm 23 is disposed in the box 21 to be movable up and down to adjust the position, and the structure of the suspension arm 23 and the manner of disposing the suspension arm in the box 21 are simple prior art and will not be described in detail; the white light lamp 24 and the infrared lamp 25 are preferably provided, and the white light lamp 24 and the infrared lamp 25 may be provided at an upper portion (not shown) outside the soundproof case 2 or inside the soundproof case 2; corresponding to the suspension arm 23, a white light lamp 24 and an infrared lamp 25 are respectively fixed on the suspension arm 23 in a manner that the light emitting points of the white light lamp 24 and the infrared lamp 25 face downward, wherein the white light lamp 24 is used for illumination, and the infrared lamp 25 is used for matching with an industrial camera for shooting.
The rotary disc 3 is a rotatable disc-shaped structural member, and the rotary disc 3 can adopt a manual rotary disc or an electric rotary disc, preferably an electric rotary disc. The rotary plate 3 is a commercially available member, and its structure will not be described in detail. The turntable 3 is provided in the soundproof box 2 and is located below the white light lamp 24 and the infrared lamp 25 of the soundproof box 2.
The behavior box 1 is provided on the turntable 3, and specifically, the behavior box 1 is provided on the turntable 3 in such a manner that the lower end surface of the bottom plate 11 thereof meets the upper end surface of the turntable 3.
The animal behavior video analysis system 4 is a commercially available part, and is preferably a Tracking Master V3.0 animal behavior video analysis system of Shanghai Vanbu Intelligent science and technology Limited. The animal behavioural video analysis system 4 comprises a monitoring analysis back-end 41 and an industrial camera 42. The industrial camera 42 can be arranged at the upper part outside the sound insulation box 2 or in the sound insulation box 2, preferably, the industrial camera 42 is fixedly arranged on the suspension arm 23 and is positioned right above the behavior box 1, and the industrial camera 42 is used for shooting the running track of the mouse under the cooperation of the infrared lamp 25 and sending the running track to the monitoring analysis background 41 during the experiment. The monitoring and analysis back-end 41 is used for the automatic analysis of the experimental data and the control of the device of the present embodiment. Specifically, the monitoring and analyzing background 41 is used for controlling each electric shock board 12-4, the white light lamp 24, the infrared lamp 25, the industrial camera 42 and power on-off control when the electric shock board is adopted as the channel board 13-3 and the electric turntable is adopted as the turntable 3 during the experiment, receiving and storing video information of the movement of the mouse in the behavior box 1 sent by the industrial camera 42 and carrying out post analysis.
The electric shock plates 12-4, the aisle plates 13-3 when the electric shock plates are adopted, the white light lamps 24, the infrared lamps 25, the rotary disc 3 when the electric rotary disc is adopted and the industrial camera 42 are all electrically connected with the monitoring analysis background 41. The structure and the working principle of the animal behavior video analysis system 4 and the electrical connection relationship between the relevant components are the prior art and are not described in detail.
The device of space and environmental memory ability under detection mouse negative emotion of this embodiment, it is when using, can adopt different normal forms to detect the experiment to the mouse according to the needs that detect the experiment, when every normal form detects the experiment, all start animal behavior video analytic system 4's control analysis backstage 41 and industrial camera 42, white light lamp 24 and infrared lamp 25 are opened to the environment when needing, utilize industrial camera 42 to carry out whole shooting monitoring, utilize control analysis backstage 41 to carry out experimental data analysis to the video that industrial camera 42 uploaded.
The paradigm one is: double-box paradigm for detecting only fear memory capacity of mouse environment
The wall plate 12-3 of 1 detection box body 12 in 3 detection box bodies 12 of the behavior box 1 is selected to be the wall plate with the transverse stripes and the door 12-3 of the behavior box is opened, the wall plate 12-3 of the other 1 detection box body 12 is selected to be the wall plate with the vertical stripes and the door 12-3 of the behavior box is opened, and the 3 rd detection box body 12 does not need to close the door 12-3 of the behavior box, so that the behavior box 1 is changed from a three-box detection experiment paradigm to a two-box detection experiment device. Compare in two box detection device of the same kind among the prior art, this device can drive action case 1 rotation through carousel 3 and get rid of the influence that spatial information caused the experiment in the two case preference experiments of prior art completely. The specific method of the paradigm comprises the following steps:
the first day: the mouse is placed into the behavior box 1 to be freely explored, the monitoring analysis background 41 carries out statistical analysis on videos uploaded by the shooting of the industrial camera 42, and background preference of the mouse on the two detection boxes 12 is measured according to accumulated exploration time of the mouse in the 2 detection boxes 12 with doors opened respectively.
The next day: putting the mice into 1 of 2 detection box bodies 12, closing a box door 12-3 of the detection box body 12, and carrying out the experiment for 45min all day; the mouse is made familiar with the environmental information of the detection box body 12, but the mouse is not matched with fear memory; after the experiment is finished, the behavior box 1 is driven by the turntable 3 to randomly rotate 120 degrees or 240 degrees so as to eliminate the influence of spatial information on the mouse recognition environment.
And on the third day: putting the mice into the other 1 of the 2 detection box bodies 12, closing the box door 12-3 of the detection box body 12, and carrying out the experiment for 45min all day; in the period, every random time of 8-10 min, the total number of electric shocks is 4-5 times, the electric shock plate 12-4 of the detection box body 12 where the mouse is located is electrified, and the mouse is given an electric shock with the current of 0.4mA for 2 s. Under this intensity of shock, the mouse only experiences fear due to the shock and does not experience pain. Through such an electric shock program, the mouse generates fear of the environment in the detection box body 12 and matches fear memory with the environment information of the detection box body 12; after the experiment is finished, the behavior box 1 is driven by the turntable 3 to randomly rotate 120 degrees or 240 degrees so as to eliminate the influence of spatial information on the mouse recognition environment.
The fourth day: and opening the doors 12-3 of the two detection boxes 12, putting the mouse into the behavior box 1 for 15min, freely exploring the mouse in the behavior box 1, respectively accumulating the exploration time of the mouse in the 2 detection boxes 12, and measuring the preference of the mouse after condition matching. Specifically, the evaluation method of the preference after condition matching is as follows: the search time of the mouse in the 2 detection boxes 12 on the first day is correspondingly subtracted from the search time of the mouse in the 2 detection boxes 12 on the fourth day, so that the judgment is carried out, and when the mouse generates negative emotion association to a certain detection box 12, the search time of the mouse in the detection box 12 is reduced.
This paradigm has got rid of spatial information's effect completely when testing, can detect the mouse better whether match fear memory and box environment to the detection box 12 of the environmental information that has matched the fear memory shows avoidance behavior.
A second paradigm: first three-box paradigm for detecting dependence priority of mice on space and environment
The wall plate 12-3 of 2 detection box bodies in 3 detection box bodies 12 of the behavior box 1 is selected from the wall plate provided with the transverse stripe, the wall plate 12-3 of the other 1 detection box body 12 is selected from the wall plate provided with the vertical stripe, the behavior box 1 is not rotated in the experimental process, and the specific method of the model comprises the following steps:
the first day: opening the doors 12-3 of 3 detection boxes 12 of the behavior box 1, placing the mouse in the behavior box 1 for 15min, freely exploring the mouse in the behavior box 1, and measuring the background preference of the mouse to the 3 detection boxes 12 by adopting the same method as the paradigm I;
the next day: placing a mouse into a detection box body 12 provided with a vertical stripe wall plate, closing a box door 12-3 of the detection box body 12, and carrying out an experiment for 45min all day; the mice are familiar with the environmental and spatial information of the test housing 12, but do not match fear memory to the mice.
And on the third day: placing the mouse into 1 of 2 detection box bodies 12 provided with transverse stripe wall boards, closing a box door 12-3 of the detection box body 12, and carrying out the experiment for 45min all day; the mice are familiar with the environmental and spatial information of the test housing 12, but do not match fear memory to the mice.
The fourth day: placing the mouse into the other 1 of the 2 detection box bodies 12 provided with the transverse stripe wall boards, and closing the box door 12-3 of the detection box body 12, wherein the experiment time of the whole day is 45 min; the same method as in paradigm one is used to shock the mouse, so that the mouse generates fear memory and matches the fear memory with the environmental and spatial information of the test housing 12.
The fifth day: and opening the box door 12-3 of each detection box body 12, putting the mouse back into the behavior box 1 for 15min, and determining the priority of the mouse on the acquisition of space and environment information after condition matching by adopting the same method as the paradigm I.
The paradigm can test the priority that the mouse obtained space and environmental information when receiving space and environmental information simultaneously. In particular, the experimental results and corresponding explanations to which the paradigm may be referenced are as follows: if the mouse shows the escape behavior of the detection box body 12 entering on the fourth day and does not show the escape behavior of the detection box body 12 entering on the third day, it indicates that the fear memory of the mouse is matched with the space and environment information at the same time, that is, the priority of the mouse on obtaining the space and environment information is similar; if the mouse shows evasion behaviors for the detection box body 12 entering the detection box body on the third day and the fourth day, it is indicated that the fear memory of the mouse is preferentially matched with the environmental information in the detection box body 12, that is, the mouse preferentially acquires the environmental information in the detection box body 12.
A paradigm three: second three-box paradigm for detecting mouse dependence on space and environment priority
The wall plate 12-3 of 2 detection box bodies 12 in 3 detection box bodies 12 of the behavior box 1 is selected as the wall plate with the transverse stripes, and the wall plate 12-3 of the other 1 detection box body 12 is selected as the wall plate with the vertical stripes. The specific method of the paradigm comprises the following steps:
the experimental method and contents of the detection from the first day to the fourth day of the present paradigm are the same as those of the second paradigm, except that:
the fifth day: the behavior box 1 is rotated by 120 degrees by utilizing the turntable 3, the box doors 12-3 of the 3 detection box bodies 12 are opened, the mouse is placed back into the behavior box 1 again for 15min, the mouse is freely explored in the behavior box 1, and the priority of the mouse on the acquisition of space and environment information after condition matching is determined by adopting the same method as the paradigm I.
This paradigm is to the further improvement of aforementioned paradigm two, there is not rotation action case 1 in paradigm two, so environment and the spatial information of every detection box 12 are fixed unchangeable to the mouse, the mouse probably matches the fear memory of space + environment when selecting, the possibility that the mouse only matches the fear memory of environment still exists, and through rotating action case 1 in paradigm three, every environment and the spatial information that detects box 12 have all taken place the change to the mouse, the mouse can only match the fear memory of space + environment when selecting, consequently for paradigm two, paradigm three has further increased the reliability of experiment.
A fourth paradigm: three-box paradigm for detecting capability of mouse to distinguish spatial information signals
The wall boards 12-3 of the 3 detection box bodies 12 of the behavior box 1 are all white wall boards or transparent wall boards covering half of the wall boards and are arranged in the same way, so that no nearby environmental information of the mouse can depend on the wall boards, and the mouse is forced to depend on the spatial information only; in the present paradigm, the white light lamp 24 in the foregoing device is not turned on, and 1 indicator lamp for distinguishing the spatial information signal from the mouse is fixedly arranged outside the upper side of the soundproof box 2, and the specific method steps of the present paradigm are as follows:
the first day: and opening the doors 12-3 of the 3 detection box bodies 12, placing the mouse in the behavior box 1 for 15min, freely exploring the mouse in the behavior box 1, and measuring the background preference of the mouse on the 3 detection box bodies 12 by adopting the same method as the paradigm I.
Second and third days: the mice are placed into 1 detection box body 12 of the 3 detection box bodies 12 repeatedly every day, the box door 12-3 of the detection box body 12 where the mice are located is closed, and the experiment time is 45min all day long; the mouse is familiar with the spatial information of the detection box body 12, but the mouse is not matched with fear memory; after each day of the experiment, the test chamber 12 was cleaned to ensure that no flavor was left, and the chamber 1 was rotated by 120 ° or 240 ° by the turntable 3 to ensure that the three test chambers 12 were adequately balanced.
The fourth day: placing the mouse into the last 1 detection box body 12 of the 3 detection box bodies 12, closing the box door 12-3 of the detection box body 12 where the mouse is located, wherein the experiment time of the whole day is 45 min; adopting the same method as the paradigm one to shock the mouse, so that the mouse generates fear memory due to the sole shock and matches the fear memory with the spatial information of the detection box body 12; after the experiment is over, the test chamber 12 is cleaned to ensure that no flavor remains, and the chamber 1 is rotated by 120 or 240 by the turntable 3 to ensure that the three test chambers 12 are adequately balanced.
The fifth day: and opening the doors 14 of the three detection boxes of the behavior box 1, putting the mouse into the behavior box 1 for 15min, freely exploring the mouse in the behavior box 1, and determining the preference of the mouse on the three detection boxes 12 after condition matching by adopting the same method as the paradigm I.
This paradigm has got rid of environmental information's effect completely when the test, can detect the mouse better and whether match the spatial information of fear memory and box and pilot lamp to show the behavior of evading to the detection box 12 of the spatial information who has matchd the fear memory.
The above embodiments are illustrative of specific embodiments of the present invention, and are not restrictive of the present invention, and those skilled in the relevant art can make various changes and modifications without departing from the spirit and scope of the present invention to obtain corresponding equivalent technical solutions, and therefore all equivalent technical solutions should be included in the scope of the present invention.
Claims (10)
1. A device for detecting the space and environment memory ability of a mouse under negative emotion comprises an animal behavior video analysis system which comprises a monitoring analysis background and an industrial camera electrically connected with the monitoring analysis background, and is characterized in that: the device also comprises a sound insulation box, a rotary table which is arranged in the sound insulation box and can rotate, and a behavior box which is arranged on the rotary table and is used for accommodating a mouse to carry out a detection test when the device is used; the behavior box comprises a bottom plate, three detection box bodies which are fixedly arranged on the bottom plate, have an included angle of 120 degrees with each other and are provided with openings at the upper ends, and a middle passageway which is fixedly arranged between the lower parts of the three detection box bodies and is respectively connected with the three detection box bodies; the industrial camera is arranged in the sound insulation box or outside the sound insulation box and is positioned above the behavior box.
2. The device for detecting the spatial and environmental memory ability under the negative emotion of a mouse according to claim 1, wherein: the sound insulation box comprises a box body, a sound insulation layer arranged on the inner periphery of the box body, a white light lamp arranged outside the box body or in the box body and used for lighting when being positioned above the behavior box for use, and an infrared lamp used for assisting the industrial camera to shoot; and the white light lamp and the infrared lamp are electrically connected with the monitoring analysis background.
3. The apparatus for detecting the spatial and environmental memory ability under the negative emotion of a mouse according to claim 2, wherein: the soundproof box further comprises a suspension arm which can move up and down and is arranged in the box body in a position adjusting mode, and the industrial camera, the white light lamp and the infrared lamp are respectively fixedly arranged on the suspension arm and are located above the behavior box.
4. The device for detecting the spatial and environmental memory ability under the negative emotion of a mouse according to claim 1, wherein: the detection box body comprises 4 longitudinal beams, 3 wall plates, 1 box door and 1 electric shock plate; each longitudinal beam is provided with an inserting groove for inserting the wall plate; the lower ends of the 4 longitudinal beams are respectively fixedly connected with the bottom plate, and the two sides of each wall plate are respectively matched and spliced with the splicing grooves of the corresponding 2 longitudinal beams; the box door is arranged at the joint of the detection box body and the middle passageway in an openable and closable manner; the electric shock plates are respectively and fixedly arranged at 1 position on the lower side of the detection box body and are arranged in a suspended mode relative to the upper end of the bottom plate; the electric shock plate is electrically connected with the monitoring analysis background.
5. The device for detecting the spatial and environmental memory ability under the negative emotion of a mouse according to claim 4, wherein: the wallboard includes transparent wallboard, translucent wallboard, shelters from half transparent wallboard, white wallboard and be equipped with the wallboard of horizontal stripe and vertical stripe on each wallboard inboard.
6. The device for detecting the spatial and environmental memory ability under the negative emotion of a mouse according to claim 4, wherein: the behavior box also comprises a top connecting frame which is inserted with the upper ends of the longitudinal beams arranged on the periphery of the bottom plate; the box door is a sliding door.
7. The device for detecting the spatial and environmental memory ability under the negative emotion of a mouse according to claim 4, wherein: the middle aisle comprises 3 connecting elbows, a central support piece and a passageway plate; each connecting elbow is respectively and fixedly connected with the lower parts of a group of 2 adjacent longitudinal beams arranged on the inner side of the bottom plate, and the central support piece and the 3 connecting elbows are respectively and fixedly connected and suspended above the bottom plate; the crossing plate is fixedly arranged on the central supporting piece.
8. The apparatus for detecting the spatial and environmental memory ability under the negative emotion of a mouse according to claim 7, wherein: the aisle plate is an electric shock plate, and the upper end surface of the aisle plate is flush with the upper end surface of the electric shock plate of the detection box body; the channel passing plate is electrically connected with the monitoring analysis background.
9. The device for detecting the spatial and environmental memory ability under the negative emotion of a mouse according to claim 1, wherein: the rotary table is a manual rotary table or an electric rotary table; and when the turntable is an electric turntable, the turntable is electrically connected with the monitoring analysis background.
10. The device for detecting the spatial and environmental memory ability under the negative emotion of a mouse according to any one of claims 1 to 9, wherein: the detection box body is a square box body.
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