CN102144475B - Method for growing seedlings of robustbhesa - Google Patents
Method for growing seedlings of robustbhesa Download PDFInfo
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- CN102144475B CN102144475B CN2010106064136A CN201010606413A CN102144475B CN 102144475 B CN102144475 B CN 102144475B CN 2010106064136 A CN2010106064136 A CN 2010106064136A CN 201010606413 A CN201010606413 A CN 201010606413A CN 102144475 B CN102144475 B CN 102144475B
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- seedling
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- robustbhesa
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/28—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture specially adapted for farming
Abstract
The invention discloses a method for growing seedlings of robustbhesa. The robustbhesa has been listed to the plants under state protection (category II) in the List of Rare and Endangered Plants in China (in 1987) and the China Plant Red Data Book (in 1992) and has been listed to the highly-endangered level of red alarming class 'Almost Disappeared (the number of the existing plants is no more than 10)' in the Threatened Quantitative Evaluation Level of Wild Plants in China. After a long-term observation, no natural seedling of the population is found and the natural breeding is difficult to carry out, which are the endangered reasons for the species. By the adoption of chemical and physical means in the invention, an environment appropriate to breeding and growing seedlings of the robustbhesa is constructed, so that the planting rate of the seedling growth in vitro culture reaches 15-50%, the planting rate of the seed seedling growth reaches 60-71%, therefore, the endangered tree species is saved, the factory massive seedling growth of the robustbhesa is realized and a new choice to the tree variety is provided for green seedlings of the tropical coast areas in China.
Description
Technical field
The present invention relates to is the seedling-cultivating method of plant bhesa sinensis, belongs to plant cultivation seedling growing process field.
Background technology
Chinese Second Class Key Protected Plant bhesa sinensis Bhesa robusta (Roxb.) D.Hou is the Celastraceae (Celastraceae) found in Hepu of Guangxi county Nankang town in 1979 in China's plant species that newly distributes, and once names to be magnificent storehouse forest (Kurrimia sinica H.T.Chang et S.Y.Liang) or bhesa sinensis (Bhesa sinica H.T.Chang et S.Y.Liang).(45 (3): 147,149.1999) record: only there is 1 strain veteran in the Hepu County of bhesa sinensis in the southeast, Guangxi, is distributed in Vietnam, India and Malaysia abroad according to " Chinese Plants will ".Bhesa sinensis is put into Chinese Second Class Key Protected Plant in " Chinese rare and endangered protective plant register " (1987) and " Chinese Plants Red Data Book " (1992), and bhesa sinensis has been put into the high-risk grade of warning red class " almost disappearance (existing plant≤10 strains) " in " the compromised qualitative assessment grade of Chinese wild plant ".Malaysia calls red-flowered spindle tree to Bhesa robusta, and the bhesa sinensis in Guangxi flower is spent little yellow green for gathering umbrella garden awl inflorescence, and is obviously variant between these two geographical population.
Bhesa sinensis is grown on not far low height above sea level sand ground of bank off sea or the tableland, more than ten meter of the height of tree, and blade approaches keratin, the spire salmon pink, annual height of seedling can reach 1 meter, is the green tree species of one's native land that the torrid zone, China south China seashore has the development utilization prospect.But also do not find the natural seedling of this population through long-term observation; Naturally breed and be difficult to carry out; Perhaps the endangered reason of these seeds that Here it is is in wild state because this tree is planted in China and south east asia simultaneously, and not seeing has relevant seedling propagating technology report.
Critical Condition and the potential value of this kind in the seashore greening of the torrid zone, south China in view of bhesa sinensis; With the non-tube rapid propagation technology platform of plant serves as rely on to implement the technical method that the bhesa sinensis batch production grows seedlings on a large scale not only aspect the species conservation important value to be arranged, and bright prospects is also arranged at species aspect utilizing.
Summary of the invention
The object of the invention has just provided a kind of bhesa sinensis seedling-cultivating method; Solved bhesa sinensis population irreproducible problem under nature; Also solved the technical problem of growing seedlings and producing, rescued endangered species, for China's regional greening of torrid zone seashore provides new choice of tree species with seedling.
The foregoing invention purpose realizes through following technical proposals: the bhesa sinensis seedling-cultivating method comprises the steps:
From the field or the nursery obtain bhesa sinensis propagating materials explant; With concentration is 0.1-0.3% (W/V) sodium chloride solution; The oxidisability electricity function water that after electrolysis, obtains is OPR900-1200mv, and the inferior hypochloric acid water of PH2.0-3.0 carries out spray disinfectant to explant;
2. with concentration root-inducing powder ABT1 solution soaking explant lower end 1-4 hour of 50-200ppm;
Explant transplanted a cutting before 1 hour the electricity consumption function water to the seedbed, greenhouse by 50～500ml/m
2Amount is carried out spray disinfectant, and it is all moistening to be sprayed to seedling medium, and the clear water atomizing passes through wet seedbed; Implant explant in the moistening perlite seedling medium in seedbed, carry out the control and management of growth of seedling environment, satisfy the root best habitat conditions of needs of explant; 18.4 ℃～33.0 ℃ of environmental temperatures between nursery stage; Ambient humidity 68～90%, 21.9 ℃～32.0 ℃ of substrate temperature, intensity of illumination 1700～7800lux;
4. grow seedlings in 10 days of initial stage; Every morning connects the high voltage electric field generator and opens the activation of 80,000 volts of high voltage electric field 0.5-1.5 hours promotion plant cells, promotes growth and pesticide and disinfectant; Nurse young plants in hothouses and spray 1～2 time 0.5～2 ‰ potassium dihydrogen phosphates and 0.001 ‰ Nafusaku solution by the amount that every young plant sprays the 5-50 milliliter 5-20 days early stage, to promote the development growth of its root system;
5. after nursing young plants in hothouses 20 days, the every 5-10 days amounts with every young plant 5-50 milliliter are used the urea nutrient solution of a 1.0-3.0%, the explant cottage propagation through growth in 30-60 days after, obtain growing new blade and a large amount of adventive root seedling;
6. sprigging is continued to grow seedlings in the nursery, land for growing field crops in nutritious bag; Rearing condition is temperature 10-35 ℃ in nutritious bag; Humidity 60-80%; Illumination 1700-41000lux can obtain the bhesa sinensis commercial seedling that height of seedling is the afforestation that is applicable to the south China coastal region of 40-100cm after growing seedlings through 1 year field.
7. the soil of the nutrition in nutritious bag prescription is: pond sludge 80-100 weight portion, gold zone mud 80-100 weight portion, mushroom residue 3-5 weight portion, animal dung 2-4 weight portion, phosphate fertilizer 1-2 weight portion; Getting above-mentioned prescription composition fully mixes; Being dried to water content is 8-15%, and the fine powder pack that is ground into particle diameter and is the 1.0-5.0 millimeter is subsequent use.
8. working as the bhesa sinensis propagating materials is kind of a period of the day from 11 p.m. to 1 a.m, then soaks kind of vernalization 24 hours with clear water earlier, is undertaken by above-mentioned steps 3-6 again, obtains the bhesa sinensis commercial seedling.
Take the present invention of above-mentioned measure; Utilize chemistry and physical means, built the environment of suitable bhesa sinensis breeding and seedling, make the explant planting percent of growing seedlings reach 15-50%; The seed seedling-raising planting percent has reached 60-71%; Saved endangered species, realized that the bhesa sinensis batch production grows seedlings on a large scale, for the greening in seashore area, China torrid zone provides new choice of tree species with seedling.The inventive method is scientific and reasonable, implements easily, and is with low cost, social benefit and remarkable in economical benefits.
Embodiment
Embodiment 1
Explant is grown seedlings: two bud cuttings of clip bhesa sinensis semi-lignified one leaf, and the lower end otch is cut into 45 degree angles near the bud below, and the upper end is cut into flat mouthful above bud.The electricity consumption function water is sprayed the explant surface, soaks bhesa sinensis cutting lower end 4 hours with No. 1 (ABT1) 100ppm of root-inducing powder after 20 minutes.Start intelligent management system, the plant characteristic that systematic parameter is set is that short-day, happiness are fertile, happiness is wet, fast numerous initial progress is set for urging 0 phase of root, temperature 25-35 ℃.The electricity consumption function water is utilized atomizing system abundant moistening seedbed expanded perlite matrix to intelligent seedbed spray disinfectant.Medium of seedling bed is inserted in the bhesa sinensis cutting to be cultivated; Continuous 5 day every morning opened 80,000 volts of high voltage electric fields 1 hour; Transplanting the 10th day and the 15th day plain otch cell activation, the callus of promoting of formulated for administered power form and differentiation the 20th day, 25 days and 30 days formulated for administered nutrient solution promotion seedling developments.18.4 ℃～33.0 ℃ of nurturing period environmental temperatures, 21.9 ℃～32.0 ℃ of substrate temperature, intensity of illumination 1700～7800lux, ambient humidity 38～90%.10～15d grows young leaves, and lower end, 20 days left and right sides otch forms callus, forms indefinite root system in 30～40 days, obtains complete vegetative propagation seedling, and planting percent reaches 45%.Sprigging is gone in the nutritious bag, and each nutritious bag is equipped with 1 kilogram of nutrition soil, and nutrition soil is the following weight proportioning: 40 kilograms in pond sludge, 47 kilograms in gold zone mud, 1.5 kilograms of mushroom residues, 1 kilogram of animal dung, phosphate fertilizer are prepared from for 0.5 kilogram, move the garden again and grow seedlings.
Embodiment 2
Seed propagation seedling: the bhesa sinensis seed of gathering spring, airing fruit, removing pericarp are removed foreign material and are obtained clean seed.In climatic cabinate, implement seed germination experiment, the result is a germination rate 60%, germination vigor 40%.Seed sprays electric work ability water sterilization 20 minutes, in clear water, soaks vernalization 24 hours.The electricity consumption function water is to intelligent seedbed spray disinfectant and abundant moistening medium of seedling bed, scatters seeds then and covers fully with matrix, opens the intelligent environment management system.Preceding 5 day every morning after planting opened 80,000 volts of high voltage electric fields 1 hour, and the ambient temperature and humidity of nurturing period, substrate temperature and illumination condition are identical with the explant rearing condition.The 10th day, 15 days, 20 days and 30 days totally 4 formulated for administered nutrient solutions promote growth of seedling.Through about 60 days cultivation, can obtain the bhesa sinensis seedling of mean height 13.8cm, emergence rate 70%.Sprigging is gone in the nutritious bag, and each nutritious bag is equipped with 1 kilogram of nutrition soil, and nutrition soil is by the following weight proportioning: 50 kilograms in pond sludge, 60 kilograms in gold zone mud, 1.5 kilograms of mushroom residues, 1 kilogram of animal dung, 0.5 kilogram in phosphate fertilizer, be prepared from, and move the garden again and grow seedlings.1 year about 80cm of height of seedling can reach 1.5 meters through the heights of seedling in 1.5 years.
Claims (3)
1. the bhesa sinensis seedling-cultivating method is characterized in that described method comprises the steps:
(1) from the field or the nursery obtain bhesa sinensis propagating materials explant; With concentration is 0.1-0.3% (W/V) sodium chloride solution; The oxidisability electricity function water that after electrolysis, obtains is OPR900-1200mv, and the inferior hypochloric acid water of PH2.0-3.0 carries out spray disinfectant to explant;
(2) with concentration be root-inducing powder ABT1 solution soaking explant lower end 1-4 hour of 50-200ppm;
(3) explant transplanted a cutting before 1 hour the electricity consumption function water to the seedbed, greenhouse by 50～500ml/m
2Amount is carried out spray disinfectant, and it is all moistening to be sprayed to seedling medium, and the clear water atomizing passes through wet seedbed; Implant explant in the moistening perlite seedling medium in seedbed, carry out the control and management of growth of seedling environment, satisfy the root best habitat conditions of needs of explant; 18.4 ℃～33.0 ℃ of environmental temperatures between nursery stage; Ambient humidity 68～90%, 21.9 ℃～32.0 ℃ of substrate temperature, intensity of illumination 1700～7800lux;
(4) grow seedlings in 10 days of initial stage; Every morning connects the high voltage electric field generator and opens the activation of 80,000 volts of high voltage electric field 0.5-1.5 hours promotion plant cells, promotes growth and pesticide and disinfectant; Nurse young plants in hothouses and spray 1～2 time 0.5～2 ‰ potassium dihydrogen phosphates and 0.001 ‰ Nafusaku solution by the amount that every young plant sprays the 5-50 milliliter 5-20 days early stage, to promote the development growth of its root system;
(5) nursed young plants in hothouses 20 days after, the every 5-10 days amounts with every young plant 5-50 milliliter are used the urea nutrient solution of a 1.0-3.0 ‰, the explant cottage propagation through growth in 30-60 days after, obtain growing new blade and a large amount of adventive root seedling;
(6) sprigging is continued to grow seedlings in the nursery, land for growing field crops in nutritious bag; Rearing condition is temperature 10-35 ℃ in nutritious bag; Humidity 60-80%; Illumination 1700-41000lux can obtain the bhesa sinensis commercial seedling that height of seedling is the afforestation that is applicable to the south China coastal region of 40～100cm after growing seedlings through 1 year field.
2. bhesa sinensis seedling-cultivating method according to claim 1; It is characterized in that the nutrition soil prescription in the nutritious bag is: pond sludge 80-100 weight portion, gold zone mud 80-100 weight portion, mushroom residue 3-5 weight portion, animal dung 2-4 weight portion, phosphate fertilizer 1-2 weight portion; Getting above-mentioned prescription composition fully mixes; Being dried to water content is 8-15%, and the fine powder pack that is ground into particle diameter and is the 1.0-5.0 millimeter is subsequent use.
3. bhesa sinensis seedling-cultivating method according to claim 1 is characterized in that said bhesa sinensis propagating materials explant replaces with the bhesa sinensis kind period of the day from 11 p.m. to 1 a.m, then soaks kind of vernalization 24 hours with clear water earlier, is undertaken by above-mentioned steps 3-6 again, obtains the bhesa sinensis commercial seedling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106064136A CN102144475B (en) | 2010-12-27 | 2010-12-27 | Method for growing seedlings of robustbhesa |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010106064136A CN102144475B (en) | 2010-12-27 | 2010-12-27 | Method for growing seedlings of robustbhesa |
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| CN102144475A CN102144475A (en) | 2011-08-10 |
| CN102144475B true CN102144475B (en) | 2012-08-08 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102986444B (en) * | 2012-12-31 | 2014-01-08 | 江苏省中国科学院植物研究所 | Method for enabling woody plant material to form adventitious root and grow into own-rooted seedling |
| CN104322226A (en) * | 2013-11-30 | 2015-02-04 | 钟诚 | Pine tree cutting seedling raising method |
| CN106171990B (en) * | 2016-07-15 | 2018-08-28 | 莫竹承 | Bhesa sinensis endosperm evoked callus method |
| CN113455273B (en) * | 2021-08-12 | 2022-05-10 | 广西红树林研究中心 | Method for cultivating seashore plant |
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2010
- 2010-12-27 CN CN2010106064136A patent/CN102144475B/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| 莫竹承 等.濒危植物膝柄木生存现状及其恢复对策.《广西科学院学报》.2008,第24卷(第2期),134-137. * |
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Granted publication date: 20120808 Termination date: 20171227 |