CN102114202A - Liver-protecting composition and preparation method thereof - Google Patents

Abstract

The invention discloses a liver-protecting composition and a preparation method thereof, and the liver-protecting composition comprises the following raw materials: Chinese jujube, hawthorn fruit, coix seed, liquoric root and Mongolian dandelion herb. The prepared liver-protecting composition can be prepared into any one common formulation for internal use and health care product, has the effects of protecting liver, expelling toxin and improving fatty liver, has the pharmacological effects of reducing aminotransferase and inhibiting hepatocellular injury, and can play great roles in protecting the liver and preventing hepatofibrosis in daily applications.

Description

A kind of composition and method of making the same that protects the liver

Technical field

The invention belongs to food and production field thereof, particularly relate to and a kind of liver is had antiinflammatory, detoxifcation (lowering the transaminase raises and lactic acid dehydrogenase), immunomodulating, anti-hepatic fibrosis, anti-liver injury effect, can have the health product and the production method thereof that protect the liver toxin expelling and improve the effect of fatty liver.

Background technology

Liver is the digestive gland of human body maximum, also is maximum body of gland, its not only secretion of bile participate in digestion activity, and nutritious substance metabolism, store glycogen, detoxify, engulf important function such as defence, also have hemopoietic function the period of embryo.

Liver is the vitals of human body, and a lot of functions are arranged, and the biochemical reaction that carries out in the liver reaches more than 500 kinds, and its major physiological function is mainly reflected in the following aspects:

1) metabolic function: liver plays an important role for the metabolism of a lot of materials such as human body internal protein, saccharide, lipid.The decomposition of protein, fat and sugar class and synthetic and co-conversion mutually between them etc. realize in liver that mainly because three major nutrient can be emitted lot of energy when decomposing, therefore, liver also is a heat production organ.In addition, liver also participates in the metabolism of vitamin, and vitamin such as A, B, C, D and K's is synthetic all closely related with liver with storage.Liver is obvious, and can vitamin metabolism to occur when impaired unusual.

2) Detoxication: liver can by oxidation and bonded mode, become material nontoxic or that toxicity is less with noxious substance with the noxious substance that enters in the body or produced in the metabolic process in vivo.Be hydrolyzed to aminoacid as the body internal protein, aminoacid decomposes the ammonia that produces, and becomes avirulent carbamide to be excreted by urine through the liver role transformation.Endogenous or ectogenic noxious substance make its toxicity disappear, weaken or combination through hepatocellular effect mostly, and the material that is converted into solubility is beneficial to discharge.

3) phagocytosis: hepatic sinusoid, the sternzellen of Dou Bishang have the antibacterial of engulfing, foreign body and old and feeble erythrocytic effect, so defense function is arranged.

4) synthetic manufacturing and storage function and 5) secretion and excretory function: hepatocyte constantly generates bile acid and secretion of bile.Bile can promote fat in enteral digestion and absorption in digestion process.

Liver be the human body vitals be again the organ of a fragility, just as protect bad can causing a disease.After virus was invaded liver, the capillary permeability of liver increased, hepatocellular degeneration swelling, and the liver internal hemorrhage, inflammatory cell infiltration causes the liver enlargement, the normal function decline.Most of hepatopathy can be cured, but the minority protracted course of disease becomes chronic hepatitis.

At present, various on the market have protect the liver, the health product and the Chinese medicine preparation of liver protection effect, most medicament that relates to or the botanical herbs in the health product are not the prescription complexity, adopt extracts such as higher Chinese herbal medicine of economic worth such as Cordyceps, Pollen Pini, Ganoderma as raw material exactly, have that the too high and raw material supply of production cost is unsettled, control of product quality is difficult, the shortcoming of weak curative effect, and owing to the medicine that uses can not be taken for a long time, therapeutical effect can only be played, hepar damnification can not be used to prevent.In recent years, various hepatopathys and hepatopathy patient in early stage presents the characteristics of sustainable growth and rejuvenation, and the market demand is a kind of easy to use, cheap and have health product of fine health-care effect.

Summary of the invention

Primary and foremost purpose of the present invention is to provide a kind of composition and method of making the same that is used to protect the liver at the problem that above-mentioned prior art exists, present composition prescription is simple, the raw material that uses is the medical material of integration of edible and medicinal herbs, have protect the liver, hepatoprotective and improve the effect of fatty liver, protect the liver the curative effect height, the present invention protects the liver that the preparation of compositions method is simple, stable processing technique is simple and direct, easy to use, cheap

For realizing purpose of the present invention, one aspect of the present invention provides a kind of compositions that protects the liver, and comprises following raw material: Fructus Jujubae, Fructus Crataegi, Semen Coicis, Radix Glycyrrhizae and Herba Taraxaci.

Wherein, the described weight portion proportioning that protects the liver composition material is: Fructus Jujubae: 0.1～10, Fructus Crataegi: 2～20, Radix Glycyrrhizae: 0.5～15, Herba Taraxaci: 0.5～10, Semen Coicis: 1～30.

Particularly, the weight portion proportioning of described raw material is: Fructus Jujubae: 0.5～5, Fructus Crataegi: 5～10, Radix Glycyrrhizae: 0.5～5, Herba Taraxaci: 0.5～5, Semen Coicis: 5～20.

The present invention protects the liver compositions and selects to carry out combined preparation by Fructus Jujubae, Fructus Crataegi, Semen Coicis, Radix Glycyrrhizae and Herba Taraxaci with antiinflammatory, detoxifcation, immunomodulating, anti-hepatic fibrosis, anti-liver injury effect and integration of edible and medicinal herbs and form, soothing liver and strengthening spleen, blood circulation promoting and blood stasis dispelling, strengthening the body resistance, heat-clearing and toxic substances removing, these integration of edible and medicinal herbs Chinese crude drugs are used in combination, make the active component in each medical material produce synergism, mutual enhancement protects the liver, the effect of hepatoprotective, thereby can suppress, stop the damage of liver organization effectively.

Fructus Jujubae has another name called Fructus Jujubae, dried Fructus Jujubae, Chinese date, is the dry mature fruit of Rhamnaceae jujube Fructus Jujubae Ziziphus jujuba Mill.var.inermis (Bunge) Rehd..Fructus Jujubae is warm in nature, and sweet in the mouth is returned spleen, stomach warp, has invigorating the spleen and replenishing QI, and the effect of nourishing blood to tranquillize the mind is mainly used in the insufficiency of the spleen lack of appetite of treatment, weak loose stool, woman hysteria.

Mainly contain alkaloid in the Fructus Jujubae: for example stepharine, N-demethyl nuciferine, asimilobine etc.; The triterpenic acid compounds: for example Betulonic acid, oleanolic acid, Crataegolic acid, 3-O-trans right-coumaric acyl maslinic acid, 3-O-cis be to coumaric acyl maslinic acid, betulic acid etc.; Oside compound: for example Fructus Jujubae soap formula I, II, III, jujuboside B etc.; Also contain cyclic adenosine monophosphate, cyclic guanosine monophosphate, fructose, glucose, sucrose, sitosterol, stigmasterol, desmosterol, globulariacitrin, riboflavin, thiamine, carotene, nicotinic acid and several amino acids.

Modern pharmacological research is found, the multiple bioactive substance that contains in the Fructus Jujubae as jujube polysaccharide, flavonoid, saponins, triterpenes, alkaloids, cyclic adenosine monophosphate, cyclic guanosine monophosphate etc., has the plurality kinds of health care effect of curing the disease to human body.

Cyclic adenosine monophosphate in the Fructus Jujubae participates in cell division and differentiation, form formation, glycogen and multiple physiological and biochemical procedures such as steatolysis, steroid generation in vivo, and can act on genetic transcription, influences proteinic synthetic.Cyclic adenosine monophosphate has significant curative effect to treating various tumors, can effectively stop the formation of nitrites material in the human body, thereby the formation of anticancer and propagation, even the material that cancerous cell is transformed to normal cell, in addition, the triterpenoid compound that is rich in the Fructus Jujubae has the effect of anticancer, and is the strongest with Crataegolic acid effectiveness especially, even surpassed anticarcinogen fluorouracil commonly used; The soap-like matter that Fructus Jujubae contains has effects such as the body metabolism of adjusting, enhancing immunity, antiinflammatory, antiallergic action, blood sugar lowering and cholesterol level; Fructose in the Fructus Jujubae, glucose, oligosaccharide, acidic polysaccharose participate in hepatoprotective.Fructus Jujubae can make the rabbit anteserum total protein and the albumin of carbon tetrachloride liver damage obviously increase.Fructus Jujubae can improve monocytic phagocytic function in the body simultaneously, and the effect of the liver protecting, physical strength reinforcing is arranged; Vitamin C in the Fructus Jujubae and cAMP etc. can alleviate the infringement of chemicals to liver, and the promotion protein synthesis is arranged, and increase the effect of The Total albumen content.Fructus Jujubae can be used for the auxiliary treatment of chronic hepatitis and early stage liver cirrhosis clinically.Jujube polysaccharide has tangible complement activity and promotes the lymphopoiesis effect, but human body immunity improving power.

Fructus Crataegi another name red fruit, TANGLIZI are the dry mature fruit of rosaceous plant Fructus Pyri Pashiae Crataegus pinnatifidaBge.var.major N.E.Br. or Fructus Crataegi Crataegus pinnatifida Bge..Slightly warm in nature, sour in the mouth, sweet is returned spleen, stomach, Liver Channel, has promoting digestion and invigorating the stomach, and the circulation of qi promoting dissipating blood stasis is mainly used in meat stagnation, gastral cavilty distension, dysentery stomachache, blood stasis amenorrhea, postpartum stagnation, trusted subordinate's twinge, hernia pain; Hyperlipemia.The effect of Fructus Crataegi (parched to brown) dyspepsia and intestinal stasis relieving strengthens.Be used for meat stagnation, dysentery is not well.

Contain left-handed youngster in the Fructus Crataegi and show caffein, Quercetin, hyperin, chlorogenic acid, Crataegolic acid, citric acid, citric acid mono-methyl, citric acid dimethyl ester, citric acid trimethyl, flavan polymers, amygdalin, sucrose, ursolic acid.Pharmacological research to Fructus Crataegi shows that Fructus Crataegi has the facilitating digestion effect, and the lipase that it contains can promote fat digestion, and can increase the secretion of peptic digestion enzyme, facilitating digestion.Gastrointestinal function is had certain regulating action, the hyperactive duodenum smooth muscle of exempting from is inhibitory action, in the face of lax rat stomach smooth muscle has slight enhancing contraction; Effect for reducing fat, Fructus Crataegi extract and pure extractum 0.5mg/kg are oral to make lecithin ratio raising in the atherosclerosis rabbits blood, and cholesterol and the lipid deposition on organ reduces.After Cavia porcellus was taken the Fructus Crataegi water decoction, cholesterol is contained into enzyme activity inhibitory action, can make the hydroxymethyl glutaryl shop enzyme A reductase vitality of its hepatocyte microsome and mucous membrane of small intestine descend about 70% and 67% respectively.The Fructus Pyri Pashiae aqueous extract can show reduction serum total cholesterol content, its effect is showing greater than pantethine, and can obviously increase HDL-C in the serum, HDL2-C, HDL3-C concentration, its effect may be that the drainage that increases cholesterol realizes by HDL and subfraction concentration thereof in the raising serum; Fructus Crataegi also has antioxidation and improves immunity function, the continuous 9d of injection subcutaneous injection administration that makes of the water decoction-alcohol sedimentation of Fructus Crataegi for example, rabbit anteserum lysozyme activity, serum coagulation antibody titre, painstaking effort T lymphocyte E rosette forming rate and T lymhocyte transformation rate are all being shown strengthen, prompting has immunological enhancement.

Semen Coicis is called Semen Coicis, Semen Coicis, seed of Job's tears, ditch rice again, is the dry mature kernal of grass Semen Coicis Coixlacryma-jobi L.var.ma-yuen (Roman.) Stapf.Semen Coicis is cool in nature, and sweet in the mouth, light is returned spleen, stomach, lung meridian, has the spleen invigorating eliminating dampness by diuresis, eliminating impediment antidiarrheal, and the function that heat clearing away row is dense is mainly used in the treatment edema, beriberi, dysuria, arthralgia chiefly caused by damp pathogen contracture, diarrhea due to hypofunction of the spleen, lung abscess, intestinal carbuncle; Verruca plana.Contain 16.2% protein in the Semen Coicis, 4.65% fat, 79.17% carbohydrate, vitamin B1 (330 microgram %) and aminoacid (leucine, lysine, arginine, tyrosine etc.), Coixol, coixenolide, triterpenoid, wherein, triacylglycerol 61%-64% in the lipid, diacylglycerol 6%-7%, monoacylglycerol 4%, sterol ester 9%, free fatty 17%-18%; Also contain the glucosan of anticomplementary action and Semen Coicis polysaccharide (coixan) A, B, the C of acidic polysaccharose CA-1, CA-2 and hypoglycemic activity.

Modern pharmacological research shows that Semen Coicis has antitumor action, and the ethanol extraction of tumor-bearing mice lumbar injection Semen Coicis can suppress the propagation of ehrlich carcinoma (ECA) cell, is showing the life span that prolongs animal; The Semen Coicis acetone extract also has obvious inhibitory action to cervical cancer 14 (U14) and ascitic type liver cancer (HCA) solid tumor; The neutral polysaccharide glucosan mixture and the acidic polysaccharose IIa-1 that get from the Semen Coicis hot water extract, 2,3, the IIb part all shows anticomplementary activity, has the effect of tangible enhance immunity ability.

Radix Glycyrrhizae is the dry root of glycyrrhizic legume Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. Glycyrrhiza glabra L., and property is flat, sweet in the mouth, GUIXIN, lung, spleen, stomach warp have invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, the effect of coordinating the actions of various ingredients in a prescription, be mainly used in weakness of the spleen and stomach, fatigue and weakness, shortness of breath and palpitation, cough with copious phlegm, gastral cavity abdomen, the anxious pain of extremity contraction, carbuncle sore tumefacting virus, cushion toxicity, strong.

Main chemical compositions in the Radix Glycyrrhizae is a triterpenoid saponin: glycyrrhizin, uralsaponin A, B and glycyrrhizin A3, B2, C2, D3, E2, F3, G2, H2, J2, K2 etc.; Flavone compounds: liquiritin, liquiritigenin, liquiritin, isoliquiritin, different Radix Glycyrrhizae unit, neoliquriitin, neoisoliquiritin, licoricidin (+)-Licoricidin., the sharp ketone of Radix Glycyrrhizae, formoononetin, ononin, Isolicoflavonol. etc.; Coumarins chemical compound: Radix Glycyrrhizae coumarin, glycyol, isoglycyol, Radix Glycyrrhizae coumarin-7-methyl ether, neoglycyrol, Radix Glycyrrhizae pyrans coumarin, licocoumarone etc.; Alkaloid: 5,6,7,8-tetrahydrochysene-4-methylquinoline, 5,6,7,8-tetrahydrochysene-2,4-dimethyl quinoline, 3-methyl-6,7,8-three hydrogen pyrrolo-es [1,2-a] pyrimidine-3-ketone etc.; Also contain licobenzofuran, sitosterol, Radix Glycyrrhizae glucosan GBW, Angelica Polysaccharide UA, UB, UC, polysaccharide GR-2a, GR-2IIb, GR-2IIC and polysaccharide GPS etc.

Radix Glycyrrhizae has blood fat reducing and the anti-atherogenic stalk turns usefulness into: its glycyrrhizin that contains has the effect of certain reduction blood cholesterol per capita to the hypertension of experimental high-cholesterol disease of rabbit and cholesterol rising.Glycyrrhizin 10mg/kg every day intramuscular injection continuous 5 days, has tangible effect for reducing fat to experimental rabbit hyperlipemia; Antitumor action: the OberlingGuerin myeloma that enoxolone is transplanted rat has inhibitory action, the mixture of monoammonium glycyrrhizinate, Monosodium glycyrrhetin and enoxolone derivative, white mice ehrlich carcinoma and sarcoma all there is inhibitory action, even oral also effective.Glycyrrhizin, liquiritin can produce morphologic variation to rat ascites hepatocarcinoma and mice ehrlich ascites cell, and glycyrrhizin still can suppress the yoshida sarcoma of subcutaneous transplantation; Liver protection effect: glycyrrhizin can stop the rising of carbon tetrachloride poisoning rat glutamate pyruvate transaminase, reduces accumulating of the interior triglyceride of liver.Histopathology is observed, and light through its hepatic injury of rat of glycyrrhizin, enoxolone treatment, histochemistry is observed and shown, the rat hepatic glycogen of enoxolone treatment obviously increases the serum tire first globulin recall rate height of glycyrrhizin and enoxolone; Promote the bile secretion effect: glycyrrhizin can increase the biliary pore bile secretion, and glycyrrhizin 5mg/kg can show the bile secretion that increases rabbit, and the red matter of gallbladder behind the rabbit ligation bile duct is raise inhibitory action.

Herba Taraxaci claims Herba crotalariae albidae, grandmother's fourth again, for feverfew Herba Taraxaci Taraxacum mongolicumHand.-Mazz., alkali ground Herba Taraxaci Taraxacum sinicum Kitag. or belong to the dry herbs of several plants together.Herba Taraxaci is cold in nature, and bitter in the mouth, sweet is returned liver, stomach warp, has heat-clearing and toxic substances removing, dispersing swelling and dissipating binds, and the effect of inducing diuresis for treating stranguria syndrome is mainly used in the treatment furuncle swelling toxin, acute mastitis, scrofula, conjunctival congestion, pharyngalgia, lung abscess, acute appendicitis, jaundice due to damp-heat, the puckery pain of pyretic stranguria.Herba Taraxaci contains taraxasterol, taraxol, taraxerol, ψ-Portugal's Herba Taraxaci sterol, β-Amyrin, stigmasterol, cupreol, choline, organic acid, fructose, sucrose, glucose, inulin, phylloxanthin, violaxanthin, flavoxanthin, phylloquinone, vitamin pectin etc.

The pharmacological action of Herba Taraxaci mainly contains antibacterial action: staphylococcus aureus Resistant strain, Hemolytic streptococcus are had stronger bactericidal action, Diplococcus pneumoniae, meningococcus, diphtheria corynebacterium, bacillus pyocyaneus, dysentery bacterium, Bacillus typhi etc. and micrococcus catarrhalis are also had certain bactericidal action; The lactogenesis effect: Herba Taraxaci has the obstruction of dredging newborn vascular, promotes the effect of lactogenic; Antitumor action and choleretic effect are used for tumor and chronic gallbladder spasmolytic and calculosis.

The present invention provides a kind of preparation of compositions method that protects the liver on the other hand, comprises the steps:

1) prepare raw material according to following weight portion proportioning:

Fructus Jujubae: 0.1～10, Fructus Crataegi: 2～20, Radix Glycyrrhizae: 0.5～15, Herba Taraxaci: 0.5～10, Semen Coicis: 1～30

2) with behind the raw material mix homogeneously, mixture is carried out heating extraction at least 2 times, collect extracting solution;

3) extracting solution of collecting is concentrated, make active component.

Wherein, the weight portion proportioning of described raw material is: Fructus Jujubae: 0.5～5, Fructus Crataegi: 5～10, Radix Glycyrrhizae: 0.5～5, Herba Taraxaci: 0.5～5, Semen Coicis: 5～20.

Wherein, heating extraction is carried out according to following steps step 2):

A) add water to raw mix, carry out heating extraction, wherein, the weight of the water of adding is 6-12 with the ratio of raw mix gross weight: 1;

B) collect each time extracting solution and merging.

Particularly, in described steps A) in, after described raw mix adds water, soaked 20-90 minute, carry out described heating extraction again.

Especially, the heating extraction temperature is 60-100 ℃, and the time is 1-4 hour.

Wherein, the described extracting solution of step 3) concentrates and carries out according to following steps: at first the extracting solution of collecting is carried out centrifugal treating, obtain supernatant; Then supernatant is carried out concentration, wherein, the concentration temperature is 60-100 ℃.

Particularly, described concentration is a concentrating under reduced pressure, wherein the relative pressure in the concentrating under reduced pressure process be-0.05～-0.095MPa.

Wherein, in described step 3), it is 1.1～1.7 extractum that described extracting solution is concentrated into relative density, is preferably 1.2～1.5.

Another aspect of the invention provide a kind of by above-mentioned arbitrary described protect the liver that the combination of acceptable auxiliary on compositions and the pharmaceutics makes protect the liver, the pharmaceutical preparation of hepatoprotective.

Wherein, the compositions that protects the liver of the present invention can be made clinical acceptable peroral dosage form by tradition or modern production technology with acceptable auxiliary on the pharmaceutics.As capsule, tablet, granule,, pill, oral liquid etc.Used excipient substance can be starch, dextrin, lactose, microcrystalline cellulose, gelatin, sucrose, magnesium stearate etc.Need long-term prescription clinically, make solid preparation and store conveniently, the patient takes facility, also meeting the market requirement more.Preferred dosage form is capsule, granule, tablet.

Further aspect of the present invention provides that a kind of above-mentioned arbitrary described compositions protects the liver in preparation, hepatoprotective and improve the application of the medicine and the health product of fatty liver.

Further aspect of the present invention provides a kind of preparation method of liver-protecting tea, comprises following step of carrying out:

1) prepare raw material according to following weight portion proportioning:

Fructus Jujubae: 0.1～10, Fructus Crataegi: 2～20, Radix Glycyrrhizae: 0.5～15, Herba Taraxaci: 0.5～10, Semen Coicis: 1～30

2) with behind the raw material mix homogeneously, mixture is carried out heating extraction at least 2 times, collect extracting solution;

3) extracting solution of collecting is concentrated, make active component;

4) in active component, add Folium Camelliae sinensis, make liver-protecting tea.

Wherein, the weight portion proportioning of raw material is: Fructus Jujubae: 0.5～5, Fructus Crataegi: 5～10, Radix Glycyrrhizae: 0.5～5, Herba Taraxaci: 0.5～5, Semen Coicis: 5～20.

Wherein, heating extraction is carried out according to following steps step 2):

A) add water to raw mix, carry out heating extraction, wherein, the weight of the water of adding is 6-12 with the ratio of raw mix gross weight: 1;

B) collect each time extracting solution and merging.

Particularly, in described steps A) in, after described raw mix adds water, soaked 20-90 minute, carry out described heating extraction again.

Especially, the heating extraction temperature is 60-100 ℃, and the time is 1-4 hour.

Wherein, the described extracting solution of step 3) concentrates and carries out according to following steps: at first the extracting solution of collecting is carried out centrifugal treating, obtain supernatant; Then supernatant is carried out concentration, wherein, the concentration temperature is 60-100 ℃.

Particularly, described concentration is a concentrating under reduced pressure, wherein the relative pressure in the concentrating under reduced pressure process be-0.05～-0.095MPa.

Wherein, in described step 3), it is 1.1～1.7 extractum that described extracting solution is concentrated into relative density, is preferably 1.2～1.5.

Wherein, step 4) also comprises spissated active component is dissolved in the alcoholic solution that mass percent concentration is 80-90% and left standstill 10-12 hour, be 1.1～1.7 supernatant extractum with supernatant concentration to relative density then, supernatant extract dry, pulverizing back add Folium Camelliae sinensis.

Particularly, described Folium Camelliae sinensis is selected one or more in green tea, scented tea, black tea, the Folium camelliae assamicae.

Wherein, the weight portion proportioning of supernatant extractum and Folium Camelliae sinensis is 1: 1-5.

The present invention protects the liver compositions and has following advantage:

1, the present invention protects the liver compositions prescription compatibility science, and determined curative effect is safe, quality controllable;

2, the present invention protects the liver the effect that compositions has soothing liver and strengthening spleen, blood circulation promoting and blood stasis dispelling, strengthening the body resistance, heat-clearing and toxic substances removing, active component in each medical material produces synergism, can promote human body immunity improving power, regulate and enhancing human body metabolism function, strengthen protect the liver, the effect of hepatoprotective, anti-hepatic fibrosis, thereby can suppress, stop the damage of liver organization effectively, reach the effect for the treatment of both the principal and secondary aspects of a disease;

3, the present invention protects the liver the Chinese crude drug that the combination of Chinese medicine material is an integration of edible and medicinal herbs, can take for a long time, has no side effect, and it is rapid that the hepatic of preparation or health product are eliminated clinical symptoms, and the effect of transaminase lowering does not significantly rebound;

4, to protect the liver the preparation of compositions method simple in the present invention, the process conditions gentleness, and with low cost, expense is low.

The specific embodiment

Below further set forth the beneficial effect of medicine of the present invention by testing example, these test the routine pharmacodynamics test that has comprised medicine of the present invention.

1 pair of fatty liver rat of test example index is improved test

Test material:

Protect the liver granule, 2g/ bag (every bag contains crude drug 10g); Positive drug: colchicine, lot number: the accurate word of medicine is defended in Yunnan, and (1996) No. 002722, manufacturer: Xishuangbanna, Yunnan pharmaceutical factory.

Test method:

Get cleaning level female sd inbred rats, 100, body weight 100～120g, fed continuously 40 days with food rich in fat, be diagnosed as fatty liver through the fatty liver corresponding index, be divided into 5 groups at random, 20 every group, promptly protect the liver 3 groups of granule low dose group (80mg/kg), middle dosage group (160mg/kg), high dose group (320mg/kg); Blank group: normal saline 320mg/kg; Positive drug group: colchicine 0.11mg/kg.

Protect the liver granule and positive drug after water is carried, be prepared into aseptic suitable concentration solution according to dosage, drug solution and contrast normal saline are all irritated stomach by the 10ml/kg per os and are given animal subject, once a day, continuous 30d, detect glutamate pyruvate transaminase (ALT), cholesterol (CH) and triglyceride (TG) index situation, result of the test sees Table 1.

The influence of table 1 pair fatty liver Mouse Liver function (X ± S)

Annotate: compare * P＜0.05, * * P＜0.01 with model group

Testing result explanation: protect the liver granule height, middle dosage and can significantly reduce and detect glutamate pyruvate transaminase (ALT), cholesterol (CH) and triglyceride (TG) content, show to have obvious improve fatty liver liver function and blood lipids index effect.

The protection test of 2 pairs of concanavalin A, Con A induced mice of test example immunologic liver injury

Test material:

Protect the liver granule, 2g/ bag (every bag contains crude drug 10g); Positive drug: bifendate drop pill, lot number: the accurate word (2001) of medicine is defended No. 002802 in the capital, manufacturer: Beijing XieHe medicine Factory.

Test method:

Get cleaning level NIH mice, 100, body weight 22～25g is divided into 5 groups at random, 20 every group, promptly protects the liver 2 groups of granule low dose group (40mg/kg), high dose group (160mg/kg); Normal control group: normal saline 320mg/kg; Model group: canavaline 20mg/kg; Positive drug group: bifendate drop pill 150mg/kg.

Protect the liver granule and positive drug after water is carried, be prepared into aseptic suitable concentration solution according to dosage.Except that the normal control group, all the other mices are all irritated stomach, every day 1 time by the 1.2ml/kg per os in testing the first day with 20mg/kg dosage intravenous injection concanavalin A, Con A with drug solution and contrast normal saline.For three days on end, detect plasma A LT activity, IFN-γ and TNF-alpha content, experimental result sees Table 2.

The influence of immune hepatic injury mice plasma ALT, IFN-γ and TNF-α due to the table 2 pair canavaline (X ± S)

Annotate: compare * P＜0.05, * * P＜0.01 with model group

The testing result explanation: protect the liver granule high dose group mice ALT activity, IFN-γ and TNF-alpha content and all be starkly lower than model group, difference has significance (t=5.19,3.63,15.90, P＜0.05,0.01); Protect the liver granule high dose group and bifendate group relatively, the difference of mice ALT activity, IFN-γ and TNF-alpha content does not have the significance meaning, shows that protecting the liver granule has the better protect effect to canavaline induced mice immunologic liver injury.

The protection test of 3 pairs of hepatocyte fibrosiss of test example rat hepatocytes

Test material:

Protect the liver granule, 2g/ bag (every bag contains crude drug 10g); Colchicine, lot number: the accurate word (1996) of medicine is defended No. 002722 in Yunnan, manufacturer: Xishuangbanna, Yunnan pharmaceutical factory.

Test method:

Get a cleaning level female sd inbred rats, 120, body weight 100～120g is divided into 6 groups at random, 20 every group, promptly protects the liver 2 groups of granule low dose group (80mg/kg), middle dosage group (160mg/kg), high dose group (320mg/kg); Normal control group: normal saline 320mg/kg; Model group; Positive drug group: colchicine 0.11mg/kg.Except that the normal control group, all the other are respectively organized rat and use 10%CCl ₄Olive oil solution 5ml/kg subcutaneous injection 2 times weekly, in continuous 13 weeks, makes Liver Fibrosis Model.The isopyknic normal saline of normal control group subcutaneous injection.After the modeling 60 days, every group irritate stomach normal saline, normal saline respectively every day, protect the liver granule (80mg/kg), protect the liver granule (160mg/kg), protect the liver granule (320mg/kg), colchicine (1mg/kg), once a day, continuous 60 days, corner of the eyes venous blood collection carries out liver function test behind the anesthetized rat, get liver lobus sinister same area after putting to death rat, the preparation paraffin section carries out liver histological and observes.Analysis of experimental data adopts SPSS10.0 software to carry out one factor analysis of variance.Experimental result sees Table 3, table 4.

The variation of table 3 Serum ALT and AST (X ± S)

Annotate: compare * P＜0.05, * * P＜0.01 with model group

The testing result explanation: compare with the normal control group, the active of model group Serum ALT and AST obviously raises; With model group relatively, protect the liver the release (P＜0.01) that can obviously reduce ALT and AST behind the granule therapy, can reach normal level, therapeutical effect is better than the colchicine group.

Table 4 serum albumin, globulin, the proteic variation of Archon (X ± S)

Annotate: compare * P＜0.05, * * P＜0.01 with model group

The explanation of table 4 testing result: compare with normal group, the model group serum albumin levels obviously lowers, and globulin content raises, and the ratio of albumin and globulin reduces; Compare with model group; protect the liver the high serum albumin of granule therapy group gram liter; reduce serum globulin; raising albumin is than (P＜0.01); relatively there is not significant difference (P＞0.05) with normal group; be better than the colchicine group, prompting protects the liver granule to be had the hepatocyte injury of alleviating, protects hepatocellular effect.

Om observation: normal rats lobules of liver structural integrity is clear, the liver plate with the central vein be the center be streak to around radial arrangement, irregular sinus hepaticus is arranged between plate, only have a little collagen fiber to exist in portal area and central vein.The most normal leaflet structures of model group destroy or disappear, and stretch out thick collagen fiber bar rope by portal area and central vein and cut apart, and hold lobules of liver, the hepatic cords arrangement disorder, and swelling of liver cell is obvious, and steatosis is extensive, and part has necrosis.Fiber has cellular infiltrations such as a large amount of monokaryons, lymph, eosinophilic granulocyte and fibroblast every interior.Compare with model group; protect the liver the granule height, in the structural deterioration of 2 dosage treatment group lobules of liver obviously alleviate; liver collagen fiber hypertrophy also obviously alleviates; the ribbon rope is loose to narrow down; the hepatocyte edema takes a turn for the better, and the steatosis situation is obviously improved, and inflammatory cell infiltration reduces; show that protecting the liver granule has the protection hepatocyte injury, improves the effect of liver structure.

Test example 4 pairs of hepatocyte fibrosiss rats'liver function and blood serum designated object influence test

Test material:

Protect the liver granule, 2g/ bag (every bag contains crude drug 10g); Colchicine, lot number: the accurate word (1996) of medicine is defended No. 002722 in Yunnan, manufacturer: Xishuangbanna, Yunnan pharmaceutical factory.

Test method:

Get a cleaning level female sd inbred rats, 120, body weight 100～120g is divided into 6 groups at random, 20 every group, promptly protects the liver 3 groups of granule low dose group (80mg/kg), middle dosage group (160mg/kg), high dose group (320mg/kg); Normal control group: normal saline 320mg/kg; Model group; Positive drug group: colchicine 0.11mg/kg.Except that the normal control group, all the other group rats are used 10%CCl ₄Olive oil solution 5ml/kg subcutaneous injection 2 times weekly, in continuous 13 weeks, makes Liver Fibrosis Model.The isopyknic normal saline of normal control group subcutaneous injection.After the modeling 60 days, every group irritate stomach normal saline, normal saline respectively every day, protect the liver granule low dose group (80mg/kg), protect the liver dosage group (160mg/kg) in the granule, protect the liver granule high dose group (320mg/kg), colchicine (1mg/kg), once a day, continuous 60 days, corner of the eyes venous blood collection carries out HA, LN behind the anesthetized rat, C-IV, PC-III measure, and analysis of experimental data adopts SPSS10.0 software to carry out one factor analysis of variance.Experimental result sees Table 5.

Table 5 is respectively organized the activity change (X ± S) of HA, LN, C-IV, PC-III

Group

Dosage (mg/kg)

Number of animals (n)

HA (ng/dl)

PC-III (ng/dl)

LN (U/ml)

C-IV (ng/dl)

The normal control group

/

20

146.1±18.60

75.25±20.14

59.89±10.00

34.00±8.72

Model group

/

20

473.2±30.82

232.22±31.17

99.60±12.33

103±24.71

The positive drug group

1

20

323.9±53.66**

147.7±12.36**

72.13±9.23

35.0±10.32

Low dose group

80

20

295.1±18.64

134.65±9.55

85..25±11.64

52.72±13.4

Middle dosage group

160

20

230.6±14.90

96..53±13.58

72.13±9.23

35.0±9.62

High dose group

320

20

265.4±53.66**

129.8±10.43

79.88±15.66

48.5±10..32

Annotate: compare * P＜0.05, * * P＜0.01 with model group

The testing result explanation: the activity of each medication group HA, LN, C-IV, PC-III and model group more all have decline (P＜0.01) in various degree, especially protect the liver dosage group in the granule and obviously reduce the activity of C-IV, PC-III, relatively there were significant differences (P＜0.01) with the colchicine group.Show that protecting the liver granule has clear and definite therapeutical effect to experimental hepatic fibrosis, can reduce degree of hepatic fibrosis, effectively alleviate stem cell injuries simultaneously, improve liver function.

In order to study the toxic action that Chinese medicine composition of the present invention may exist body, acute toxicity test and long term toxicity test have been carried out.

5 acute toxicity tests of test example

Choosing NIH is 20 of healthy mices, and body weight is the 18-20 gram, male and female half and half.First fasting 12 hours before the experiment during experiment, with the dose of the tolerant Cmax of white mice, maximum volume, protects the liver granule with the present invention and is made into 30% concentration, according to 15g/kg body weight gastric infusion 1 time, observes 7 days.Experimental result is not measured Chinese medicine composition of the present invention to mice LD50 value, this dosage is equivalent to 1153 times of clinical dosage, medicine of the present invention gives the maximum tolerated dose of mice lavage administration greater than 15g/kg, shows that medicine of the present invention or functional food do not have acute toxic reaction, and safety is good.

Test example 6 long term toxicity tests

After animal feeding observed for 1 week, selecting every index normal N IH is 96 of healthy mices, is divided into high dose group (0.18g/kg), middle dosage group (0.36g/kg), low dose group (0.54g/kg) and matched group at random, 24 of every treated animals, male and female half and half, body weight are 18-20g.During off-test, randomly draw 20 for every group and measure every index, residue is put to death and is cutd open inspection.

High, medium and low dosage group dosage is equivalent to 40 times, 80 times, 160 times of clinical consumption, is equivalent to 1,2,4 times of pharmacodynamics effective dose respectively.Protect the liver granule with the preparation of distilled water suspension, press 44mg/kg, 88mgl/kg, 176mgkg body weight per os gastric infusion, every day 2 times, matched group gives the starch suspension of equivalent.Detect index, and be aided with the pathological examination that animal is respectively organized internal organs, the toxicity situation of overall merit medicine with general performance, body weight, hematology and serum biochemistry index before and after the animals administer, routine urinalysis, organ weights equipotential.

The result shows: medicament capsule of the present invention changes the no significance difference opposite sex to the weight of animals, and animal growth is normal; Every inspection index of high, medium and low dosage treated animal shows no obvious abnormalities; The result of histopathologic examination shows that three dosage treated animals are respectively organized the internal organs no abnormality seen.Show that medicine of the present invention is not good at the phase toxic reaction, be safe medication.

The present invention will be further described below in conjunction with embodiment.

Embodiment 1 the present invention protects the liver particulate preparation

1) prepare raw material (kg) according to following weight proportion:

Fructus Jujubae 0.5, Fructus Crataegi 10, Radix Glycyrrhizae 0.5, Herba Taraxaci 5, Semen Coicis 5

2) each constitutive material mix homogeneously is placed in the Chinese medicine multi-function extractor, adds tap water and soak 30min, wherein the weight of the tap water of Jia Ruing is 6: 1 with the ratio of raw material gross weight;

3) open the multi-function extractor power supply and heat, be heated to 100 ℃, remain on that constant temperature extracted 4 hours under this temperature, filter then, obtain that extracting solution is standby for the first time;

Heating-up temperature is not limited to 100 ℃, and other temperature all are applicable to the present invention.

4) add tap water in filtering residue, the weight of the tap water of adding is 6: 1 with the ratio of raw material gross weight, and heating extraction, heating-up temperature are 100 ℃, and extraction time is 4 hours, filters to obtain extracting solution for the second time;

5) 2 extracting solution are merged the back and adopt centrifuge to carry out centrifugal treating, obtain supernatant;

6) supernatant is carried out reduction vaporization and concentrate, the relative vacuum degree in the concentration process be-0.07MPa, and temperature is 80 ℃, and it is 1.2 extractum that supernatant concentration is become relative density, carries out drying then under 45-50 ℃, pulverizes the back and crosses 180 mesh sieves, gets the dry extract powder;

7) the dry extract powder is mixed with the adjuvant dextrin, alcohol granulation, drying promptly makes and protects the liver granule.

Wherein, the heating extraction temperature is not limited to 100 ℃, and heating-up temperature all is applicable to the present invention at 60-100 ℃; Constant temperature extraction time was not limited to 4 hours, and extraction time all was applicable to the present invention at 1-4 hour.

Embodiment 2 the present invention protect the liver the preparation of sheet

1) prepare raw material (kg) according to following weight proportion:

Fructus Jujubae 5, Fructus Crataegi 5, Radix Glycyrrhizae 5, Herba Taraxaci 0.5, Semen Coicis 20

2) each constitutive material mix homogeneously is placed in the Chinese medicine multi-function extractor, adds tap water and soak 20min, wherein the weight of the tap water of Jia Ruing is 12: 1 with the ratio of raw material gross weight;

3) open the multi-function extractor power supply and heat, be heated to 60 ℃, remain on that constant temperature extracted 1 hour under this temperature, filter then, obtain that extracting solution is standby for the first time;

Heating-up temperature is not limited to 60 ℃, and other temperature all are applicable to the present invention.

4) in filtering residue, divide the adding tap water 3 times, heating extraction 3 times, the temperature of each heating extraction is 60 ℃, and the weight of the tap water of adding is 12: 1 with the ratio of raw material gross weight, and extraction time is 1 hour, filters to obtain extracting solution 3 times;

5) above-mentioned 4 extracting solution are merged the back and adopt centrifuge to carry out centrifugal treating, obtain supernatant;

6) supernatant is carried out reduction vaporization and concentrate, the relative vacuum degree in the concentration process be-0.07MPa, and temperature is 80 ℃, and it is 1.5 extractum that supernatant concentration is become relative density, carries out drying then under 45-50 ℃, pulverizes the back and crosses 100 mesh sieves, gets the dry extract powder;

7) the dry extract powder is mixed with adjuvant, make granule, and dry, and compacting is in flakes.

Wherein, the heating extraction temperature is not limited to 60 ℃, and heating-up temperature all is applicable to the present invention at 60-100 ℃; Constant temperature extraction time was not limited to 1 hour, and extraction time all was applicable to the present invention at 1-4 hour.

Embodiment 3 the present invention protect the liver particulate preparation

1) prepare raw material (kg) according to following weight proportion:

Fructus Jujubae 2.5, Fructus Crataegi 5, Radix Glycyrrhizae 2.5, Herba Taraxaci 2.5, Semen Coicis 15

2) each constitutive material mix homogeneously is placed in the Chinese medicine multi-function extractor, adds tap water and soak 90min, wherein the weight of the tap water of Jia Ruing is 9: 1 with the ratio of raw material gross weight;

3) open the multi-function extractor power supply and heat, be heated to 80 ℃, remain on that constant temperature extracted 2.5 hours under this temperature, filter then, obtain that extracting solution is standby for the first time;

Heating-up temperature is not limited to 80 ℃, and other temperature all are applicable to the present invention.

4) in filtering residue, divide the adding tap water 2 times, heating extraction 2 times, the temperature of each heating extraction is 80 ℃, and the weight of the tap water of adding is 9: 1 with the ratio of raw material gross weight, and extraction time is 2.5 hours, filters to obtain extracting solution 2 times;

5) above-mentioned 3 extracting solution are merged the back and adopt centrifuge to carry out centrifugal treating, obtain supernatant;

6) supernatant is carried out evaporation and concentration, the relative vacuum degree in the concentration process is 0MPa, and temperature is 100 ℃, and it is 1.3 extractum that supernatant concentration is become relative density, carries out drying then under 45-50 ℃, pulverize the back and cross 100 mesh sieves, the dry extract powder;

7) in the dry extract powder, add ethanol as adhesive, add supplementary product starch and make granule.

Wherein, the heating extraction temperature is not limited to 80 ℃, and heating-up temperature all is applicable to the present invention at 60-100 ℃; Constant temperature extraction time was not limited to 2.5 hours, and extraction time all was applicable to the present invention at 1-4 hour.

The preparation of embodiment 4 liver-protecting teas of the present invention

1) prepare raw material (kg) according to following weight proportion:

Fructus Jujubae 0.5, Fructus Crataegi 10, Radix Glycyrrhizae 0.5, Herba Taraxaci 5, Semen Coicis 5

2) each constitutive material mix homogeneously is placed in the Chinese medicine multi-function extractor, adds tap water and soak 60min, wherein the weight of the tap water of Jia Ruing is 9: 1 with the ratio of raw material gross weight;

3) open the multi-function extractor power supply and heat, be heated to 100 ℃, remain on that constant temperature extracted 4 hours under this temperature, filter then, obtain that extracting solution is standby for the first time;

Heating-up temperature is not limited to 90 ℃, and other temperature all are applicable to the present invention.

4) add tap water in filtering residue, heating extraction, the temperature of heating extraction are 100 ℃, and the weight of the tap water of adding is 9: 1 with the ratio of raw material gross weight, and extraction time is 4 hours, filter to obtain extracting solution the 2nd time;

5) above-mentioned 2 extracting solution are merged the back and adopt centrifuge to carry out centrifugal treating, obtain supernatant A;

6) supernatant A is carried out evaporation and concentration, the relative vacuum degree in the concentration process is-0.07MPa, and temperature is 80 ℃, and it is 1.2 extractum A that supernatant concentration is become relative density;

7) adding mass percent concentration in extractum A is 80% alcoholic solution, leaves standstill after the stirring and dissolving 12 hours, filter supernatant B, and carrying out evaporation and concentration, to become relative density be 1.2 extractum B, under 45-50 ℃, carry out drying then, pulverize the back and cross 180 mesh sieves, get dry extract powder B;

8) add green tea in dry extract powder B, mix homogeneously is made liver-protecting tea, and wherein the weight proportion of dry extract powder B and green tea is 1: 1.

Wherein, the heating extraction temperature is not limited to 100 ℃, and heating-up temperature all is applicable to the present invention at 60-100 ℃; Constant temperature extraction time was not limited to 4 hours, and extraction time all was applicable to the present invention at 1-4 hour.

The preparation of embodiment 5 liver-protecting teas of the present invention

1) prepare raw material (kg) according to following weight proportion:

Fructus Jujubae 5, Fructus Crataegi 5, Radix Glycyrrhizae 5, Herba Taraxaci 0.5, Semen Coicis 20

2) each constitutive material mix homogeneously is placed in the Chinese medicine multi-function extractor, adds tap water and soak 90min, wherein the weight of the tap water of Jia Ruing is 12: 1 with the ratio of raw material gross weight;

3) open the multi-function extractor power supply and heat, be heated to 60 ℃, remain on that constant temperature extracted 1 hour under this temperature, filter then, obtain that extracting solution is standby for the first time;

Heating-up temperature is not limited to 60 ℃, and other temperature all are applicable to the present invention.

4) in filtering residue, divide 3 to add tap water, heating extraction 3 times, the temperature of each heating extraction is 60 ℃, and the weight of the tap water of adding is 12: 1 with the ratio of raw material gross weight, and extraction time is 1 hour, filters to obtain extracting solution 3 times;

5) above-mentioned 4 extracting solution are merged the back and adopt centrifuge to carry out centrifugal treating, obtain supernatant A;

6) supernatant A is carried out evaporation and concentration, the relative pressure in the concentration process is 0MPa, and temperature is 100 ℃, and it is 1.5 extractum A that supernatant concentration is become relative density;

7) adding mass percent concentration in extractum A is 90% alcoholic solution, leaves standstill after the stirring and dissolving 12 hours, filter supernatant B, and carrying out evaporation and concentration, to become relative density be 1.5 extractum B, under 45-50 ℃, carry out drying then, pulverize the back and cross 100 mesh sieves, get dry extract powder B;

8) add scented tea in dry extract powder B, mix homogeneously is made liver-protecting tea, and wherein the weight proportion of dry extract powder B and green tea is 1: 5.

Wherein, the heating extraction temperature is not limited to 60 ℃, and heating-up temperature all is applicable to the present invention at 60-100 ℃; Constant temperature extraction time was not limited to 1 hour, and extraction time all was applicable to the present invention at 1-4 hour.

The preparation of embodiment 6 liver protecting capsules of the present invention

1) prepare raw material (kg) according to following weight proportion:

Fructus Jujubae 0.1, Fructus Crataegi 20, Radix Glycyrrhizae 15, Herba Taraxaci 0.5, Semen Coicis 30

2) each constitutive material mix homogeneously is placed in the Chinese medicine multi-function extractor, adds tap water and soak 60min, wherein the weight of the tap water of Jia Ruing is 12: 1 with the ratio of raw material gross weight;

3) open the multi-function extractor power supply and heat, be heated to 80 ℃, remain on that constant temperature extracted 2 hours under this temperature, filter then, obtain that extracting solution is standby for the first time;

Heating-up temperature is not limited to 80 ℃, and other temperature all are applicable to the present invention.

4) in filtering residue, divide the adding tap water 2 times, heating extraction 2 times, the temperature of each heating extraction is 80 ℃, and the weight of the tap water of adding is 12: 1 with the ratio of raw material gross weight, and extraction time is 2 hours, filters to obtain extracting solution 2 times;

5) above-mentioned 3 extracting solution are merged the back and adopt centrifuge to carry out centrifugal treating, obtain supernatant;

6) supernatant is carried out reduction vaporization and concentrate, the relative pressure in the concentration process be-0.07MPa, and temperature is 80 ℃, and it is 1.3 extractum that supernatant concentration is become relative density, carries out drying then under 45-50 ℃, pulverizes the back and crosses 100 mesh sieves, gets the dry extract powder;

7) with the dry extract powder with after the adjuvant microcrystalline cellulose mixes, the snap fit capsule of packing into is made capsule.

Wherein, the heating extraction temperature is not limited to 80 ℃, and heating-up temperature all is applicable to the present invention at 60-100 ℃; Constant temperature extraction time was not limited to 2 hours, and extraction time all was applicable to the present invention at 1-4 hour.

The preparation of embodiment 7 hepatagogues of the present invention

1) prepare raw material (kg) according to following weight proportion:

Fructus Jujubae 10, Fructus Crataegi 2, Radix Glycyrrhizae 5, Herba Taraxaci 10, Semen Coicis 1

2) each constitutive material mix homogeneously is placed in the Chinese medicine multi-function extractor, adds tap water and soak 90min, wherein the weight of the tap water of Jia Ruing is 10: 1 with the ratio of raw material gross weight;

3) open the multi-function extractor power supply and heat, be heated to 100 ℃, remain under this temperature constant temperature and mentioned 3 hours, filter then, obtain that extracting solution is standby for the first time;

Heating-up temperature is not limited to 100 ℃, and other temperature all are applicable to the present invention.

4) add tap water in filtering residue, the weight of the tap water of adding is 10: 1 with the ratio of raw material gross weight, and heating-up temperature is 100 ℃, and extraction time is 3 hours, filters to obtain extracting solution the 2nd time;

5) above-mentioned 2 extracting solution are merged the back and adopt centrifuge to carry out centrifugal treating, obtain supernatant;

6) supernatant is carried out reduction vaporization and concentrate, the relative pressure in the concentration process be-0.09MPa, and temperature is 80 ℃, and it is 1.5 extractum that supernatant concentration is become relative density, carries out drying then under 45-50 ℃, pulverizes the back and crosses 100 mesh sieves, gets the dry extract powder;

7) with the dry extract powder with after the adjuvant refined honey mixes, make hepatagogue.

Wherein, the heating extraction temperature is not limited to 100 ℃, and heating-up temperature all is applicable to the present invention at 60-100 ℃; Constant temperature extraction time was not limited to 3 hours, and extraction time all was applicable to the present invention at 1-4 hour.

Claims (10)

1. one kind protects the liver compositions, it is characterized in that comprising following raw material: Radix Glycyrrhizae, Fructus Crataegi, Fructus Jujubae, Herba Taraxaci and Semen Coicis.

2. the compositions that protects the liver as claimed in claim 1 is characterized in that the weight portion proportioning of described raw material is: Fructus Jujubae: 0.1～10, Fructus Crataegi: 2～20, Radix Glycyrrhizae: 0.5～15, Herba Taraxaci: 0.5～10, Semen Coicis: 1～30.

3. the compositions that protects the liver as claimed in claim 2 is characterized in that the weight portion proportioning of described raw material is selected: Fructus Jujubae: 0.5～5, Fructus Crataegi: 5～10, Radix Glycyrrhizae: 0.5～5, Herba Taraxaci: 0.5～5, Semen Coicis: 5～20.

4. one kind protects the liver the preparation of compositions method, comprises following step of carrying out:

1) prepare raw material according to following weight portion proportioning:

Fructus Jujubae: 0.1～10, Fructus Crataegi: 2～20, Radix Glycyrrhizae: 0.5～15, Herba Taraxaci: 0.5～10, Semen Coicis: 1～30

2) with behind the raw material mix homogeneously, mixture is carried out heating extraction at least 2 times, collect extracting solution;

3) extracting solution of collecting is concentrated, make active component.

5. preparation method as claimed in claim 4 is characterized in that the weight portion proportioning of described raw material is: Fructus Jujubae: 0.5～5, Fructus Crataegi: 5～10, Radix Glycyrrhizae: 0.5～5, Herba Taraxaci: 0.5～5, Semen Coicis: 5～20.

6. as claim 4 or 5 described preparation methoies, it is characterized in that step 2) described in heating extraction carry out according to following steps:

A) add water to raw mix, carry out heating extraction, wherein, the weight of the water of adding is 6-12 with the ratio of raw mix gross weight: 1;

B) collect each time extracting solution and merging.

7. preparation method as claimed in claim 6 is characterized in that in described steps A) in, described after raw mix adds water, soaked 20-90 minute, carry out described heating extraction again.

8. as claim 4 or 5 described preparation methoies, it is characterized in that extracting solution described in the described step 3) concentrates carries out according to following steps: at first the extracting solution of collecting is carried out centrifugal treating, obtain supernatant; Then supernatant is carried out concentration, wherein, the concentration temperature is 60-100 ℃.

9. the preparation method of a liver-protecting tea comprises following step of carrying out:

1) prepare raw material according to following weight portion proportioning:

Fructus Jujubae: 0.1～10, Fructus Crataegi: 2～20, Radix Glycyrrhizae: 0.5～15, Herba Taraxaci: 0.5～10, Semen Coicis: 1～30

2) with behind the raw material mix homogeneously, mixture is carried out heating extraction at least 2 times, collect extracting solution;

3) extracting solution of collecting is concentrated, make active component;

4) in active component, add Folium Camelliae sinensis, make liver-protecting tea.

One kind protect the liver according to claim 1 that compositions protects the liver in preparation, hepatoprotective and improve application in fatty liver medicine and the health product.