CN102093953A - Device for cell contaminated dust exposure test - Google Patents
Device for cell contaminated dust exposure test Download PDFInfo
- Publication number
- CN102093953A CN102093953A CN2010105608778A CN201010560877A CN102093953A CN 102093953 A CN102093953 A CN 102093953A CN 2010105608778 A CN2010105608778 A CN 2010105608778A CN 201010560877 A CN201010560877 A CN 201010560877A CN 102093953 A CN102093953 A CN 102093953A
- Authority
- CN
- China
- Prior art keywords
- cell
- chamber
- exposure
- dust
- cellular exposure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000428 dust Substances 0.000 title claims abstract description 35
- 238000012360 testing method Methods 0.000 title abstract description 8
- 238000004113 cell culture Methods 0.000 claims abstract description 22
- 239000000843 powder Substances 0.000 claims abstract description 17
- 238000009792 diffusion process Methods 0.000 claims abstract description 6
- 230000001413 cellular effect Effects 0.000 claims description 36
- 235000015097 nutrients Nutrition 0.000 claims description 12
- 238000002955 isolation Methods 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 239000012930 cell culture fluid Substances 0.000 claims description 4
- 230000006641 stabilisation Effects 0.000 claims description 4
- 238000011105 stabilization Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 231100000027 toxicology Toxicity 0.000 abstract description 4
- 238000010171 animal model Methods 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 3
- 210000000056 organ Anatomy 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 abstract 7
- 210000000170 cell membrane Anatomy 0.000 abstract 2
- 239000003814 drug Substances 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000005192 partition Methods 0.000 abstract 1
- 230000005180 public health Effects 0.000 abstract 1
- 238000010998 test method Methods 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 15
- 239000007789 gas Substances 0.000 description 10
- 241001465754 Metazoa Species 0.000 description 5
- 230000000694 effects Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000133 toxic exposure Toxicity 0.000 description 1
- 238000013022 venting Methods 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a device for a cell contaminated dust exposure test. The gas inlet end of a main gas pipe is connected with a dust generator and an inlet gas source, and the other end of the main gas pipe is connected with a purifying treatment device through an exhaust pipe with a flow controller. A gas inlet pipe is connected with a cell exposure chamber through a gas diffusion port, and a gas exhaust port is connected with a powder collection chamber through a gas collection port. A partition cover is arranged between the powder collection chamber and the cell exposure chamber, a cell membrane plate is arranged between the cell exposure chamber and a cell culture solution chamber, and a warm bath chamber is arranged outside the cell membrane plate. A culture solution supply device is connected with the cell culture solution chamber. The device provided by the invention has the beneficial effects that in a contaminated dust test, in vitro culture is used to replace experimental animals, the test method is optimized and improved, the culture of target organ cells or other cells can be directly used in the contaminated dust test, the whole process of the test can be conveniently controlled, and the test results are accurate. The device can be widely used in cell contaminated dust exposure test in medicine, public health environmental science, molecular biology, cytology, toxicology and other fields.
Description
Technical field
The present invention relates to a kind of cellular exposure dust exposure experimental device.
Background technology
In the scientific domains such as present stage biology, environmentalism, diseases prevention and control, medical science, military science, toxicology, suck the experiment of dust toxic exposure and also rest on animal experiment stage.This exists that the laboratory animal quantity that needs is many, experiment condition is harsh, animal individual difference causes greatly that experiment effect is not good, the cost height of testing and the requirement of animal welfare and protection aspect etc. are unfavorable for factor seriously restricting going deep into of this type of research.And the appearance of cell toxicology research and swift and violent a kind of can accurately control and the direct dust exposure experimental device of effect of development exigence.
Summary of the invention
For addressing the above problem, the invention provides a kind of cellular exposure dust exposure experimental device, get rid of the interference of other factors in the experiment, target organ is directly carried out the dust exposure experiment, and can control the experiment whole process.
For achieving the above object, technical scheme of the present invention provides a kind of cellular exposure dust exposure experimental device, comprise that main tracheae air inlet one end is connected with dust producer and inlet air source, the other end links to each other with purifying processing device by the vapor pipe that has flow director.Inlet pipe links to each other with the cellular exposure chamber by the diffusion gas port, and venting port links to each other with powder collection cavity by collecting gas port.The lid of isolation is arranged between powder collection cavity and the cellular exposure chamber, between cellular exposure chamber and the cell cultures sap cavity cell lamina membranacea is arranged, this part has temperature to bathe the chamber outward.The nutrient solution feeding mechanism links to each other with the cell cultures sap cavity.
There is the isolation lid that evenly is covered with aperture in described cellular exposure chamber, it on it powder collection cavity, be placed with the cell lamina membranacea between itself and the cell cultures sap cavity, cell lamina membranacea upper surface is exposed in the cellular exposure chamber fully, lower surface soaks in the cell cultures sap cavity, and this four part of powder collection cavity, cellular exposure chamber, cell cultures sap cavity and cell lamina membranacea is all bathed chamber institute parcel fully by temperature.
Flow director is housed respectively on dust producer, inlet air source, inlet pipe, vapor pipe.
Described inlet pipe bottom is for spreading gas port in the cellular exposure chamber, and the vapor pipe bottom is for collecting gas port in powder collection cavity.
Described temperature is bathed between control device and the temperature bath chamber to be connected by conduit and is formed the temperature control loop.
Described cell cultures sap cavity and dress row current stabilization pump between the nutrient solution feeding mechanism of nutrient solution flow director is housed, the other end is equipped with cell culture fluid water conservancy diversion delivery pipe.
Its beneficial effect is to substitute laboratory animal with the vitro culture thing in the dust exposure experiment, experimental technique is optimized and improves, can directly carry out the dust exposure experiment to cultures such as target organ cells, the whole process of control experiment easily obtains accurate experimental result.Can be widely used in the cellular exposure dust exposure experiment in each field such as medical science, public hygienic environment, molecular biology, cytology, toxicology.
Description of drawings
Accompanying drawing is the synoptic diagram of structure of the present invention.
Among the figure:
1, purifying processing device
2, vapor pipe
3, collect gas port
4, conduit
5, temperature is bathed control device
6, nutrient solution feeding mechanism
7, nutrient solution flow director
8, current stabilization pump
9, temperature is bathed the chamber
10, cell cultures sap cavity
11, cell culture fluid water conservancy diversion delivery pipe
12, cell lamina membranacea
13, cellular exposure chamber
14, powder collection cavity
15, isolate lid
16, inlet pipe
17, main tracheae
18, dust producer
19, flow director
20, inlet air source
21, diffusion gas port
Embodiment
In conjunction with the accompanying drawings the present invention is illustrated.
As shown in drawings, main tracheae (17) air inlet one end is connected with dust producer (18) and inlet air source (20), and the end of giving vent to anger links to each other with purifying processing device (1) by flow director (19).Purifying processing device (1) can carry out thorough purifying treatment with experiment waste gas and reach safety dumping.The isolation lid (15) that evenly is covered with aperture is arranged at top, cellular exposure chamber (13), its top is powder collection cavity (14), be placed with cell lamina membranacea (12) between cellular exposure chamber (13) and the cell cultures sap cavity (10), cell lamina membranacea (12) upper surface is exposed in the cellular exposure chamber (13) fully, and lower surface soaks in cell cultures sap cavity (10).The nutrient solution feeding mechanism (6) that nutrient solution flow director (7) is housed on it links to each other with cell cultures sap cavity (10), current stabilization pump (8) is housed between the two, the other end is equipped with cell culture fluid water conservancy diversion delivery pipe (11), guarantees that the required nutrition of cell growth is constantly supplied.
And powder collection cavity (14), cellular exposure chamber (13), cell cultures sap cavity (10) and this four part of cell lamina membranacea (12) are all bathed chamber (9) institute parcel fully by temperature.Temperature is bathed chamber (9) and is wrapped in whole cellular exposure chamber (13), cell lamina membranacea (12) and cell cultures sap cavity (10) outside fully, and bathes between the control device (5) with temperature and to be connected by conduit (4), and the temperature maintenance that temperature is bathed in the chamber (9) is constant.
On inlet pipe (16) on dust producer (18), inlet air source (20), the main tracheae (17) and vapor pipe (2), flow director (19) is housed respectively, can accurately controls the generating capacity of dust and concentration etc.There is diffusion gas port (21) inlet pipe (16) bottom in cellular exposure chamber (13), can fully cover table on the abundant exposing cell of interior gaseous substance that cell lamina membranacea (12) makes cellular exposure chamber (13) and; There is collection gas port (3) vapor pipe (2) bottom in powder collection cavity (14), can collect the discarded dust of being derived by the aperture of isolating on the lid (15) fully.
The structure of described herein device can be applied to a plurality of experiment modules of being made up of inlet pipe (16), vapor pipe (2), powder collection cavity (14), the cellular exposure chamber (13) that has isolation lid (15), cell lamina membranacea (12), cell cultures sap cavity (10) and temperature bath chamber (9) simultaneously.
When using, at first open cellular exposure chamber (13), the cell lamina membranacea (12) that has experimental cell will be isolated lid (15) with cultivating cover completely after being placed under the aseptic condition on the cell cultures sap cavity (10), steadily putting well.Regulate flow director (19) and nutrient solution flow director (7) then, open nutrient solution feeding mechanism (6) and temperature bath control device (5), open dust producer (18) and inlet air source (20).Dust enters main tracheae (17) and is transported to uniformly in the cellular exposure chamber (13) by diffusion gas port (21), and the upper surface that evenly spreads in experimental cell carries out the dust exposure experiment to experimental cell.The depleted dust is collected in the purifying processing device (1) by the collection gas port (3) of vapor pipe (2) bottom, carries out safe handling.
Claims (6)
1. cellular exposure dust exposure experimental device, it is characterized in that: comprise that main tracheae (17) air inlet one end is connected with dust producer (18) and inlet air source (20), the other end links to each other with purifying processing device (1) by the vapor pipe (2) that has flow director (19).Inlet pipe (16) links to each other with cellular exposure chamber (13) by diffusion gas port (21), and vapor pipe (2) links to each other with powder collection cavity (14) by collecting gas port (3).The lid of isolation (15) is arranged between powder collection cavity (14) and cellular exposure chamber (13), between cellular exposure chamber (13) and the cell cultures sap cavity (10) cell lamina membranacea (12) is arranged, this part has temperature to bathe chamber (9) outward.Nutrient solution feeding mechanism (6) links to each other with cell cultures sap cavity (10).
2. cellular exposure dust exposure experimental device according to claim 1, it is characterized in that: there is the isolation lid (15) that evenly is covered with aperture in described cellular exposure chamber (13), on it powder collection cavity (14), be placed with cell lamina membranacea (12) between itself and the cell cultures sap cavity (10), cell lamina membranacea (12) upper surface is exposed in the cellular exposure chamber (13) fully, lower surface soaks in cell cultures sap cavity (10), and powder collection cavity (14), cellular exposure chamber (13), cell cultures sap cavity (10) and this four part of cell lamina membranacea (12) are all bathed chamber (9) institute parcel fully by temperature.
3. cellular exposure dust exposure experimental device according to claim 1 is characterized in that: on dust producer (18), inlet air source (20), inlet pipe (16), vapor pipe (2) flow director (19) is housed respectively.
4. cellular exposure dust exposure experimental device according to claim 1 is characterized in that: described inlet pipe (16) bottom is for spreading gas port (21) in cellular exposure chamber (13), and vapor pipe (2) bottom is for collecting gas port (3) in powder collection cavity (14).
5. cellular exposure dust exposure experimental device according to claim 1 is characterized in that: described temperature is bathed between control device (5) and the temperature bath chamber (9) to be connected by conduit (4) and is formed the temperature control loop.
6. cellular exposure dust exposure experimental device according to claim 1, it is characterized in that: described cell cultures sap cavity (10) and be equipped with between the nutrient solution feeding mechanism (6) of nutrient solution flow director (7) current stabilization pump (8) is housed, the other end is equipped with cell culture fluid water conservancy diversion delivery pipe (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010560877 CN102093953B (en) | 2010-11-26 | 2010-11-26 | Device for cell contaminated dust exposure test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010560877 CN102093953B (en) | 2010-11-26 | 2010-11-26 | Device for cell contaminated dust exposure test |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102093953A true CN102093953A (en) | 2011-06-15 |
| CN102093953B CN102093953B (en) | 2013-11-06 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010560877 Expired - Fee Related CN102093953B (en) | 2010-11-26 | 2010-11-26 | Device for cell contaminated dust exposure test |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102093953B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105223111A (en) * | 2014-07-04 | 2016-01-06 | 中国科学院苏州纳米技术与纳米仿生研究所 | The external safety detection device of environment inhalable particles and method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19526533A1 (en) * | 1995-07-20 | 1997-01-23 | Fraunhofer Ges Forschung | Nutritional medium delivered to cell culture through permeable membrane |
| CN100458442C (en) * | 2002-05-24 | 2009-02-04 | 英美烟草(投资)有限公司 | An exposure device |
| CN101659990A (en) * | 2008-08-29 | 2010-03-03 | 北京微生物流行病研究所 | Negative selection of cell disease targets |
| CN201560188U (en) * | 2009-09-17 | 2010-08-25 | 中国烟草总公司郑州烟草研究院 | Full smoke exposure device of cigarette |
-
2010
- 2010-11-26 CN CN 201010560877 patent/CN102093953B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19526533A1 (en) * | 1995-07-20 | 1997-01-23 | Fraunhofer Ges Forschung | Nutritional medium delivered to cell culture through permeable membrane |
| CN100458442C (en) * | 2002-05-24 | 2009-02-04 | 英美烟草(投资)有限公司 | An exposure device |
| CN101659990A (en) * | 2008-08-29 | 2010-03-03 | 北京微生物流行病研究所 | Negative selection of cell disease targets |
| CN201560188U (en) * | 2009-09-17 | 2010-08-25 | 中国烟草总公司郑州烟草研究院 | Full smoke exposure device of cigarette |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105223111A (en) * | 2014-07-04 | 2016-01-06 | 中国科学院苏州纳米技术与纳米仿生研究所 | The external safety detection device of environment inhalable particles and method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102093953B (en) | 2013-11-06 |
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Address after: 300000 building 304, No. 39, Huanghai Third Street, Tianjin Economic and Technological Development Zone, Binhai New Area, Tianjin Patentee after: TIANJIN HOPE INDUSTRY & TRADE Co.,Ltd. Address before: 300020 No. 9, Xinjiang Road, Heping District, Tianjin Patentee before: TIANJIN HOPE INDUSTRY & TRADE Co.,Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131106 |