CN102090335A - Culture medium used in rooting phase of plant tissue culture - Google Patents

Culture medium used in rooting phase of plant tissue culture Download PDF

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Publication number
CN102090335A
CN102090335A CN2010105804542A CN201010580454A CN102090335A CN 102090335 A CN102090335 A CN 102090335A CN 2010105804542 A CN2010105804542 A CN 2010105804542A CN 201010580454 A CN201010580454 A CN 201010580454A CN 102090335 A CN102090335 A CN 102090335A
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CN
China
Prior art keywords
plant tissue
river sand
rooting
thin river
medium used
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2010105804542A
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Chinese (zh)
Inventor
张爱东
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DALIAN DONGFANG FRUIT AND VEGETABLE PROFESSIONAL COOPERATIVE
Original Assignee
DALIAN DONGFANG FRUIT AND VEGETABLE PROFESSIONAL COOPERATIVE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DALIAN DONGFANG FRUIT AND VEGETABLE PROFESSIONAL COOPERATIVE filed Critical DALIAN DONGFANG FRUIT AND VEGETABLE PROFESSIONAL COOPERATIVE
Priority to CN2010105804542A priority Critical patent/CN102090335A/en
Publication of CN102090335A publication Critical patent/CN102090335A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a culture medium used in rooting phase of plant tissue culture, comprising cleaned thin river sand, mineral element, nutrient element and hormone, wherein the using amount thereof is 1/4 MS. The grain diameter of the thin river sand is 0.1 to 1 mm. The invention has the following advantages: the thin river sand with zero cost is adopted to replace the agar so that the rooting speed can be increased by 30%; the quality of the root system is very good, and the root system is similar to the rooting system under soil environment. The thin sand can be used repeatedly; the fungus pollution can be reduced by 60%; and the growth of the seedling is not influenced even if being polluted.

Description

The Plant Tissue Breeding stage medium of taking root
Technical field
The present invention relates to agricultural technology field, specifically is a kind of Plant Tissue Breeding stage medium of taking root.
Background technology
The existing Plant Tissue Breeding stage medium of taking root mainly is agar+nutritive element+hormone.The nutrition flowability is poor in the agar, and gas permeability is poor, and bacterium breeds easily.Though liquid culture allows the flowability problem that has solved nutrition, but need to adopt special-purpose concussion shaking table satisfy the demand of root system to oxygen by concussion.And it is tender and crisp that agar medium is taken root, and the root hair is few.Entering the transplanting stage will shift to an earlier date hardening, and transplanting also needs to have shaded early stage, and survival rate is all below 80%.
Summary of the invention
In order to overcome above-mentioned defective, the invention provides a kind of Plant Tissue Breeding stage medium of taking root, replace agar with the thin river sand that cleans up, make the overall broth material cost reduce by 40%.
Technical scheme of the present invention is achieved in that the Plant Tissue Breeding stage medium of taking root, and contains the thin river sand, mineral element, nutritive element and the hormone that clean up, and its consumption is 1/4MS.
The particle diameter of described thin river sand is 0.1 ~ 1mm.
The invention has the beneficial effects as follows: adopt thin river sand zero cost to replace agar, rooting rate improves 30%, and the root system quality is fabulous, being similar to takes root following of soil environment is, fine sand can also be reused, and mushroom pollute to reduce by 60%, even and polluted and also do not influence a bottle seedling growth.
Embodiment
The Plant Tissue Breeding stage medium of taking root, containing the thin river sand particle diameter that cleans up is 0.1 ~ 1mm, mineral element, nutritive element and hormone, its consumption is 1/4MS.
Adopt thin river sand zero cost to replace agar, rooting rate improves 30%, and the root system quality is fabulous, and being similar to takes root following of soil environment is, fine sand can also be reused, and mushroom pollutes and reduces by 60%, even and polluted and also do not influence bottle seedling and grow.
The above; only be the preferable embodiment of the present invention; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to replacement or change according to technical scheme of the present invention and inventive concept thereof, all should be encompassed within protection scope of the present invention.

Claims (2)

1. the Plant Tissue Breeding stage medium of taking root is characterized in that, the described Plant Tissue Breeding stage medium of taking root contains thin river sand, mineral element, nutritive element and the hormone that cleans up, and its consumption is 1/4MS.
2. the Plant Tissue Breeding according to claim 1 stage medium of taking root is characterized in that the particle diameter of described thin river sand is 0.1 ~ 1mm.
CN2010105804542A 2010-12-09 2010-12-09 Culture medium used in rooting phase of plant tissue culture Pending CN102090335A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2010105804542A CN102090335A (en) 2010-12-09 2010-12-09 Culture medium used in rooting phase of plant tissue culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2010105804542A CN102090335A (en) 2010-12-09 2010-12-09 Culture medium used in rooting phase of plant tissue culture

Publications (1)

Publication Number Publication Date
CN102090335A true CN102090335A (en) 2011-06-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN2010105804542A Pending CN102090335A (en) 2010-12-09 2010-12-09 Culture medium used in rooting phase of plant tissue culture

Country Status (1)

Country Link
CN (1) CN102090335A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102550420A (en) * 2012-01-16 2012-07-11 北京农学院 Method for preparing Damascus rose tissue culture rooting medium
CN102742508A (en) * 2012-08-08 2012-10-24 昆明琪鼎权生物科技有限公司 Method for utilizing tissue culture bag to simulate ecology to perform plant tissue culture
CN108419678A (en) * 2018-05-30 2018-08-21 楚雄师范学院 A kind of chiltern plant tissue culture media and the preparation method and application thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
王淑勤等: "用河沙和蛭石制作植物组织培养的培养基", 《河北林业科技》 *
赵建萍等: "培养因子对艾西丝南瓜芽增殖及不定根形成的影响", 《植物学通报》 *
马纯艳等: "激素及支持物对丰花月季快速繁殖的影响", 《吉林林业科技》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102550420A (en) * 2012-01-16 2012-07-11 北京农学院 Method for preparing Damascus rose tissue culture rooting medium
CN102550420B (en) * 2012-01-16 2016-07-13 北京农学院 The preparation method of Damascus rose tissue culture rooting medium
CN102742508A (en) * 2012-08-08 2012-10-24 昆明琪鼎权生物科技有限公司 Method for utilizing tissue culture bag to simulate ecology to perform plant tissue culture
CN108419678A (en) * 2018-05-30 2018-08-21 楚雄师范学院 A kind of chiltern plant tissue culture media and the preparation method and application thereof

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Application publication date: 20110615