CN102048785A - Application of eupatorium adenophorum spreng in preparation of veterinary medicine or feed additive - Google Patents
Application of eupatorium adenophorum spreng in preparation of veterinary medicine or feed additive Download PDFInfo
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Abstract
The invention relates to application of eupatorium adenophorum spreng or a eupatorium adenophorum spreng extract in preparation of a veterinary medicine for treating respiratory system diseases or preparation of a medicine for enhancing the immunity. The treatment on the respiratory system diseases comprises inflammation diminishing, cough relieving, phlegm elimination and flu or infectious bronchitis treatment. The invention further relates to application of the eupatorium adenophorum spreng or the extract thereof in preparation of a feed additive for livestock. The application realizes effective development and utilization of the eupatorium adenophorum spreng, turns the harm into a benefit, and has great economic and social significance.
Description
Technical field
The present invention relates to the application of plant Eupatorium cannabinum L. (Eupatorium coelestinum L.), be specifically related to Eupatorium cannabinum L. (Eupatorium coelestinum L.) or its extract purposes in preparation veterinary medicine or health-caring feedstuff addictive.
Background technology
Eupatorium cannabinum L. (Eupatorium coelestinum L.) (Eupatorium adenophorum Spreng) belongs to Compositae, for perennial, the evergreen fruticuli of the shape of growing thickly, is commonly called as Herba Eupatorii Odorati, devil's grass.Originate in South America Mexico, import Yunnan Province of China by Burma, Thailand's one band the forties in 20th century, begins the seventies to lead to crop smothering in China.At present, Eupatorium cannabinum L. (Eupatorium coelestinum L.) in Yunnan, Sichuan, Guizhou extensively distributes, Biomass is huge in the severely afflicated area, but does not up to the present also have the effective hierarchy of control.Eupatorium cannabinum L. (Eupatorium coelestinum L.) is effectively developed, turn bane into boon, have great economy and social meaning.
In recent years, ground such as Sichuan, Yunnan have carried out some development and use to Eupatorium cannabinum L. (Eupatorium coelestinum L.), but only limit to some roughing, mainly be used for bio-fuel, be pressed into cardboard, biogas etc., also be not fully recognized that its pharmacological function.Eupatorium cannabinum L. (Eupatorium coelestinum L.) is poisonous weeds, and the report of intoxicating phenomenon is arranged after horse, cattle, sheep and mice are edible.The inventor finds that its toxin mainly is present in the blade in the toxin of research Eupatorium cannabinum L. (Eupatorium coelestinum L.) distributes, other position content seldom or detect less than; And can effectively remove toxin in the Eupatorium cannabinum L. (Eupatorium coelestinum L.) blade by microbial fermentation.Therefore, because the Eupatorium cannabinum L. (Eupatorium coelestinum L.) aboundresources, and the toxicity problem that limits its exploitation can solve, and its development and use are well worth doing.Present discovering at Eupatorium cannabinum L. (Eupatorium coelestinum L.), contain multiple bioactive ingredients in this plant, wherein contained monoterpene, sesquiterpene and derivative compound thereof show tangible parasite killing, refusing to eat, bacteriostatic activity, and contained plant sesquiterpene, steroidal, triterpene, flavone, phenylpropyl alcohol element and derivative compound thereof have higher antibiotic, antiinflammatory, active anticancer.Understand the pharmacological action of Eupatorium cannabinum L. (Eupatorium coelestinum L.), can improve the added value of its development and use greatly.
Along with the continuous expansion of intensive culture scale, the disease of each side such as the respiratory system disease of poultry, digestive system disease is more serious.With regard to respiratory system disease, bird flu in recent years, swine flue etc. are also brought great challenge to human beings'health when influencing aquaculture.Antibiotic abuse makes the problem of Resistant strain more and more serious, also causes a large amount of drug residues simultaneously, has a strong impact on the quality of livestock and poultry meat.Employing has the ruderal plant medicine treatment respiratory illness of livestock and poultry of pharmacological action can avoid above problem, and cost is low simultaneously, wide material sources, and can turn harm into good, but do not see that at present report is arranged.
Summary of the invention
The objective of the invention is to the deficiency that exists in the above-mentioned field, provide Eupatorium cannabinum L. (Eupatorium coelestinum L.) at preparation treatment respiratory system disease veterinary medicine or as the application aspect the additive for farm animal feed.Toxin (Herba Lycopi's ketones component) content is wherein dropped to account for Radix Glycyrrhizae weight below 0.2% after extract again.
Eupatorium cannabinum L. (Eupatorium coelestinum L.) or purple stem boneset extract are in the veterinary medicine of preparation treatment respiratory system disease or the application in the preparation raising immunity medicine.
Described treatment respiratory system disease comprises antiinflammatory, cough-relieving, eliminates the phlegm, treats influenza or infectious bronchitis.
Described purple stem boneset extract is used to prepare oral formulations, and described oral formulations is capsule, powder or granule.
Eupatorium cannabinum L. (Eupatorium coelestinum L.) or its extract are as the application of additive for farm animal feed aspect.
Described feed additive addition accounts for the 0.1-20% of animal daily ration.
Account for the 0.01-5% of animal daily ration with purple stem boneset extract as the addition of feed additive, account for the 0.5-20% of animal daily ration with Eupatorium cannabinum L. (Eupatorium coelestinum L.) as the addition of feed additive, described feedstuff is used for prevention or treatment respiratory system disease.
Described Eupatorium cannabinum L. (Eupatorium coelestinum L.) is an Eupatorium cannabinum L. (Eupatorium coelestinum L.) plant shoot branch; Described purple stem boneset extract, preparation by the following method: raw material is the aerial parts of Eupatorium cannabinum L. (Eupatorium coelestinum L.), dries in the shade after gathering, pulverizing, extraction, merge extractive liquid,, concentrate drying, promptly gets purple stem boneset extract.
Adopt the compound microorganism ferments detoxification before the described extraction, described complex microorganism comprises Lactobacillus plantarum, bacillus subtilis, Bacillus licheniformis, bacillus acidophilus etc., toxin (Herba Lycopi's ketones component) content is wherein dropped to account for Radix Glycyrrhizae weight below 0.2% after extract again.When using, carry out detoxification in advance and can reduce the side effect that a small amount of toxin of containing in the Eupatorium cannabinum L. (Eupatorium coelestinum L.) blade causes as medicinal preparation for oral administration.
Described water or organic solvent or the water-containing organic solvent of being extracted as adopts lixiviate or supersound extraction or heating and refluxing extraction 1-3 time, and the 3-10 that each solvent volume consumption is a medical material weight doubly; Described concentrate drying is that decompression and solvent recovery does not have alcohol flavor postlyophilization or spray drying to doing or being concentrated into.
Described organic solvent is ethanol or methanol or the two mixture, described water-containing organic solvent is that the organic solvent volumetric concentration is the aqueous solution of 10-95%, described extraction time is 12-24h, and its time of described supersound extraction is 10-30min, and its time of described heating and refluxing extraction is 1-3h.
For a better understanding of the present invention, below with the pharmacological evaluation of the extract of Eupatorium cannabinum L. (Eupatorium coelestinum L.) and the new purposes that the result illustrates Eupatorium cannabinum L. (Eupatorium coelestinum L.):
Get Eupatorium cannabinum L. (Eupatorium coelestinum L.) aerial parts coarse powder 1Kg, lixiviate is three times under the room temperature, each 24h, the ethanol consumption is respectively 8L, 6L and 6L, merging filtrate, and decompression and solvent recovery is to doing, get purple stem boneset extract and use for following pharmacological evaluation, yield is 5.0%, and general flavone content is 21.3%.The purple stem boneset extract that obtains with Eupatorium cannabinum L. (Eupatorium coelestinum L.) or additive method also can reach same effect, and difference only is the difference of consumption.Animal experimental data represents that with x ± SD The data SPSS11.3 statistical software carries out one-dimensional variance analysis.
(1) purple stem boneset extract acute toxicity testing
40 of Kunming mouses, male and female half and half, body weight 20 ± 2g is divided into experimental group and matched group at random, 20 every group.Behind the fasting 14h.Experimental group is by the disposable purple stem boneset extract (being equivalent to former plant dry weight mice dosage is 20g/Kg) that gives of mice body weight dosage 5g/Kg, every Mus is irritated stomach 0.5mL, every mice of matched group gives solvent 1% tween 80 aqueous solution 0.5mL, observe a week continuously after the administration, find that experimental group removes that the activity that shows slightly reduces in after administration two hours, outside recovering normally afterwards gradually, whole experimental period, experimental group and matched group were all grown normally, do not have poison, dead and other abnormal phenomenas.Press food toxicology safety evaluatio experiment conclusion, Eupatorium cannabinum L. (Eupatorium coelestinum L.) dosing time amount does not have death greater than 5000mg/kg, can think that this extract toxicity is lower, need not to do lethal dose and accurately measures.
Prove that by toxicity test the toxicity of Eupatorium cannabinum L. (Eupatorium coelestinum L.) and extract thereof is very low, its ethanol extraction is equivalent to former plant LD50＞20g/Kg for LD50＞5g/Kg of mice, belongs to actual non-toxic type plant.
(2) purple stem boneset extract anti-inflammatory activity experiment
1. material
1.1 medicine: purple stem boneset extract, dexamethasone tablet (Guangdong Huanan Pharmaceutical Factory)
1.2 animal: Kunming mouse, regular grade, male and female half and half, body weight 20 ± 2g; Provide the animal quality certification number by Sichuan University's Experimental Animal Center: SC * K (111)-10-2006
2. method
The mice ear experiment: Kunming mouse is divided into 5 groups at random: blank group (giving equal-volume 1% tween 80 % aqueous solution), dexamethasone positive controls (2mgkg-1), 3 dosage groups of Eupatorium cannabinum L. (Eupatorium coelestinum L.) ethanol extraction (1g/kg, 0.5g/kg, 0.25g/kg, extract mixture is suspended from the distilled water of 1% tween 80), 12 every group.Each organizes the mouse stomach administration, every day 1 time, 7d continuously.At the front and back of mouse right ear two sided coatings proinflammatory agent dimethylbenzene (100%) 0.05mL/ only, left ear is left intact behind last administration 30min.Put to death mice behind the 30min, cut ears and lay round auricle at same position respectively with 5mm diameter card punch, weigh, every Mus auris dextra sheet weight deducts left auricle weight and is the swelling degree, and statistics is respectively organized the inhibitory rate of intumesce of ear thickness and the administration group of mice.
Inhibitory rate of intumesce (%)=(the average swelling degree of the matched group-average swelling degree of administration the group)/average swelling degree of matched group * 100%
3. result
The table purple stem boneset extract to the influence of mice ear (x ± SD, n=12)
| Group | Dosage/gkg-1 | Swelling degree/% | Swelling rate inhibition/% |
| Blank | - | 38.1±25.1 | 0 |
| The Eupatorium cannabinum L. (Eupatorium coelestinum L.) low dose group | 0.25 | 31.0±20.0 | 18.4 |
| Dosage group in the Eupatorium cannabinum L. (Eupatorium coelestinum L.) | 0.5 | 24.3±23.6 | 36.8 |
| The Eupatorium cannabinum L. (Eupatorium coelestinum L.) high dose group | 1 | 16.4±9.2* | 57.0 |
| Dexamethasone group | 2.0×10-3 | 10.7±6.5** | 72.1 |
Annotate: * and blank group be P＜0.05 relatively; * compares P＜0.01 with blank group
Purple stem boneset extract senior middle school low dose group has in various degree inhibitory action to mice ear.Compare with the blank group, the ear thickness of Eupatorium cannabinum L. (Eupatorium coelestinum L.) high dose extract mice has remarkable inhibitory action, inhibitory rate of intumesce 57%.This shows that purple stem boneset extract has certain antiinflammatory action.
(3) purple stem boneset extract immunomodulating experiment
1. material
1.1 medicine: purple stem boneset extract, dexamethasone tablet (Guangdong Huanan Pharmaceutical Factory)
1.2 animal: Kunming mouse, regular grade, male and female half and half, body weight 20 ± 2g is provided by Sichuan University's Experimental Animal Center,
The animal quality certification number: SC * K (111)-10-2006
2. method
2.1 hemolysin experiment
72 of Kunming mouses are divided into 6 groups at random, 12 every group.Normal control group, cyclophosphamide group are irritated the stomach normal saline by every mice 0.3ml every day, and all the other each groups are irritated stomach with capacity variable concentrations medicine (ring+medicine) every day, press the high, medium and low dosage (1gkg of purple stem boneset extract respectively
-1, 0.5gkg
-1, 0.25gkg
-1), after diluting one times, positive control Cordyceps Ganoderma oral liquid stock solution presses every 0.3ml administration, continuously 10d.In administration the 6th day, except that the normal control group, each group is annotated 5% chicken red blood cell 0.2ml sensitization by every mouse peritoneal, administration 8d, except that normal group, equal subcutaneous injection cyclophosphamide 0.02mgg, 3d continuously, behind the last injection 24h, get eyeball of mouse and get blood 1ml-1.5ml, leave standstill the EP pipe that 1h puts into anticoagulant, the centrifugal 10min of 2000-3000r/min, get upper serum 50 μ l, with 100 times of NS dilutions, get this serum sample 2ml and under ice bath, add 5% chicken red blood cell 1mL, add 10% complement 1ml mixing again, 37 ℃ of temperature are bathed 1h, reaction finishes, and reaction tube is moved into cessation reaction in the ice bath immediately, with the centrifugal 10min of 2000r/min,, get the 2ml supernatant in 540nm survey its absorbance (A) as serum in the index of hemolysin.(establish the not blank pipe of increase serum in addition, i.e. 5% chicken red blood cell suspension 1ml, 10% complement 1ml mixes, and is centrifugal in the refrigerator stopped reaction behind 37 ℃ of water bath heat preservation 1h, transfers 0 benchmark when getting its supernatant as colorimetric).
2.2 the exponential detection of mouse immune organ
Get when above-mentioned 2.1 mouse experiments finish and pluck eyeball sacrificed by exsanguination mice, strip spleen and thymus, and surrounding tissue is peeled off clean back its weight in wet base of title
3 results
The table purple stem boneset extract is to the immunocompromised effect of immunologic function
Annotate: * and blank group be P＜0.05 relatively; * compares P＜0.01 with blank group
Purple stem boneset extract 0.5gkg
-1And 1gkg
-1Group can significantly promote the formation of immunocompromised mice serum hemolysin, compares significant difference with the blank group, with normal group than no significant difference.1gkg
-1The dosage group can significantly improve immunocompromised mouse thymus exponential sum index and spleen index.Experimental result shows the improve effect of body's immunity of purple stem boneset extract.
(4) purple stem boneset extract cough-relieving experiment
1. material: test with immunomodulating
2. method
Mice ammonia draws coughs experiment: experiment grouping and administration experiment (2) together, fasting 12h before the experiment, and after the last administration 30min, 1 mice is placed the frosted wide mouthed bottle (adding the cotton balls that contains 75uL ammonia in advance) of 250ml, cough latent period (by putting into the bottle beginning to the time that produces cough) and the cough number of times of 2min mice of observing and write down mice (show as the contraction of mice abdominal muscle, magnify mouth simultaneously, cough sound sometimes).
3. result
| Group | Dosage/gkg-1 | Incubation period/s | Cough/time |
| Blank | - | 27.6±13.3 | 12.3±5.3 |
| The Eupatorium cannabinum L. (Eupatorium coelestinum L.) low dose group | 0.5 | 25.8±8.7 | 6.7±2.6** |
| Dosage group in the Eupatorium cannabinum L. (Eupatorium coelestinum L.) | 1 | 33.7±17.8 | 7.0±4.0** |
| The Eupatorium cannabinum L. (Eupatorium coelestinum L.) high dose group | 2 | 23.6±5.6 | 8.2±2.9* |
| The codeine phosphate group | 4×10-3 | 39.8±12.8* | 7.2±2.0** |
Annotate: * and blank group be P＜0.05 relatively; * compares P＜0.01 with blank group
Experimental result shows that the purple stem boneset extract of high, normal, basic dosage all can significantly suppress the mouse cough that ammonia causes, reduces the cough number of times, but to there not being obvious influence the incubation period of cough.
(5) the purple stem boneset extract experiment of eliminating the phlegm
1. material: test with immunomodulating
2. method
Phenol red excretion experiment: kunming mice, group technology and dosage are with experiment (2), and positive controls is aqueous ammonium chloride solution (the mice dosage is 1g/Kg), 10 every group.Behind the last administration 30min, lumbar injection 5% phenol red saline solution, 0.01mLg-1 mice.Behind the injection 30min mice is put to death, back of the body position is fixing, separates trachea, gets one section trachea (long 1cm) and puts into the test tube that the 1mL normal saline is housed, and vibration 30min sucks another in vitro with flushing liquor, adds 0.1mL 1molL-1NaOH, in 546nm place colorimetric, surveys the 0D value
3. result
| Group | Dosage/gkg-1 | Absorbance |
| Blank | - | Handle |
| The Eupatorium cannabinum L. (Eupatorium coelestinum L.) low dose group | 0.25 | Blank |
| Dosage group in the Eupatorium cannabinum L. (Eupatorium coelestinum L.) | 0.5 | The Eupatorium cannabinum L. (Eupatorium coelestinum L.) low dose group |
| The Eupatorium cannabinum L. (Eupatorium coelestinum L.) high dose group | 1 | Dosage group in the Eupatorium cannabinum L. (Eupatorium coelestinum L.) |
| The ammonium chloride group | 1 | The Eupatorium cannabinum L. (Eupatorium coelestinum L.) high dose group |
* compare P＜0.05 with the blank group; * compares P＜0.01 with blank group
Compare purple stem boneset extract 0.5gkg with the blank group
-1The dosage group can significantly improve phenol red excretion amount, shows that purple stem boneset extract can make mice expectoration ability strengthen, and has certain phlegm-dispelling functions.
The result proves by effect experiment, and Eupatorium cannabinum L. (Eupatorium coelestinum L.) has antiinflammatory, immunity, cough-relieving, expectorant activity.Control according to the theoretical dialectical opinion of Chinese veterinarian, respiratory tract disease can be considered in the epidemic disease poison invades exuberant lung-stomach heat, stagnation of QI and blood.Rule of treatment anti-inflammation, eliminating phlegm and stopping cough.Eupatorium cannabinum L. (Eupatorium coelestinum L.) has cough-relieving, eliminates the phlegm and antiphlogistic effect, and Eupatorium cannabinum L. (Eupatorium coelestinum L.) can improve the function of humoral immunization simultaneously, improves the situation of immunologic hypofunction, the treatment livestock and birds respiratory disease, and as influenza, infectious bronchitis etc. can reach the purpose for the treatment of both the principal and secondary aspects of a disease.
The concrete application of Eupatorium cannabinum L. (Eupatorium coelestinum L.) and extract thereof can comprise as follows among the present invention:
1. be used to prepare the veterinary drug of antiinflammatory, eliminating phlegm and stopping cough and raising immunity.Concrete grammar is that the Eupatorium cannabinum L. (Eupatorium coelestinum L.) of effective dose or its extract are cooperated with acceptable auxiliary on the veterinary drug, makes to be fit to the medicine that different animals uses dosage form.
Prepared veterinary medicine can be used for treating respiratory illness of livestock and poultry, as influenza, infectious bronchitis etc.
2. be used to prepare the animal feed health-care additive of antiinflammatory, eliminating phlegm and stopping cough and raising immunity.Concrete grammar is to adopt suitable process for dispersing to mix with feed formula the Eupatorium cannabinum L. (Eupatorium coelestinum L.) of effective dose or its extract, according to the general method for preparing feedstuff, is processed into health-care feed.
Prepared health-care feed can be used for prevention, treatment respiratory illness of livestock and poultry, as influenza, infectious bronchitis etc.
Comprehensive above the application promptly realized Eupatorium cannabinum L. (Eupatorium coelestinum L.) is effectively developed, and turns bane into boon, and a kind of novel poultry treatment respiratory system disease and the medicine and the feed additive of enhancing immunity are provided again.
The specific embodiment
Below the invention will be further described by specific embodiment, but content of the present invention is not limited to this fully.
Embodiment 1
Get Eupatorium cannabinum L. (Eupatorium coelestinum L.) aerial parts coarse powder 1Kg, lixiviate is three times under the room temperature, each 24h, and the ethanol consumption is respectively 8L, 6L and 6L, merging filtrate, decompression and solvent recovery gets purple stem boneset extract to doing, and yield is 5.0%, and general flavone content is 21.3%.
Embodiment 2
Get the nearly top of Eupatorium cannabinum L. (Eupatorium coelestinum L.) 30-40cm stem and leaf coarse powder (toxin composition dehydrogenation Herba Lycopi ketone content is 0.39%) 100g, (main bacteria seed is a Lactobacillus plantarum to spray 0.5% liquid complex microorganism, bacillus subtilis, Bacillus licheniformis, the bacillus acidophilus) the detoxifying agent closed processes is after three days, dry Eupatorium cannabinum L. (Eupatorium coelestinum L.) detoxification grass meal (toxin composition dehydrogenation Herba Lycopi ketone content reduces to 0.011%), the methanol supersound extraction is three times under the room temperature, each 30min, three times methanol usage is 300mL, merging filtrate, decompression and solvent recovery is to doing, get purple stem boneset extract, yield is 7.3%, and general flavone content is 19.8%.
Embodiment 3
Get florescence Eupatorium cannabinum L. (Eupatorium coelestinum L.) aerial parts coarse powder 200g, add distilled water, heating and refluxing extraction three times, each 2h, the distilled water consumption is respectively 1.6L, 1.2L and 1.2L, merging filtrate, decompression and solvent recovery is to small size, and lyophilization gets purple stem boneset extract, yield is 14.6%, and general flavone content is 25.5%.
Embodiment 4 Eupatorium cannabinum L. (Eupatorium coelestinum L.) influenza capsule for animals
Material: purple stem boneset extract 140g, detoxification Eupatorium cannabinum L. (Eupatorium coelestinum L.) fine powder 140g, carboxymethyl starch sodium 8g, Icing Sugar 40g.Extract being mixed with Eupatorium cannabinum L. (Eupatorium coelestinum L.) detoxification fine powder, add Icing Sugar and carboxymethyl starch sodium, is binding agent with the ethanol liquid of PVP, the system granule, and the dry back of 40 degree is encapsulated behind the magnesium stearate mix homogeneously, obtains 1000 of influenza capsules.
Embodiment 5 Eupatorium cannabinum L. (Eupatorium coelestinum L.) influenza granule for animals
Material: purple stem boneset extract 100g, Eupatorium cannabinum L. (Eupatorium coelestinum L.) detoxification fine powder 100g, Icing Sugar 20g, micropowder silica gel 20g, carboxymethyl starch sodium 3g, PVP 30g, ethanol 200ml, magnesium stearate 2g, Pulvis Talci 2g.Extract being mixed with Eupatorium cannabinum L. (Eupatorium coelestinum L.) detoxification grass meal, add Icing Sugar and carboxymethyl starch sodium, is binding agent with HPMC, and the system granule is drying to obtain granule.
Embodiment 6 meat rabbit trophophases health care complete feed prescription
Prescription: corn 20%, bean cake 23%, Semen Tritici aestivi 15%, wheat bran 20%, animal albumen powder 1%, premix material 1.5%, Eupatorium cannabinum L. (Eupatorium coelestinum L.) detoxification grass meal 18%, Sal 0.5%, stone powder 1%.
Embodiment 725% health care piglets concentrate feed
Prescription: bean cake 70%, cottonseed meal 4%, hyperglobulinemia 7%, imported fish meal 10%, growth pig premix 3%, purple stem boneset extract 0.5%, lysine 1%, choline chloride 0.2%, vegetable oil 1%, stone powder 2%, sweeting agent 0.2%, salt 1.1%.
Embodiment 8 broiler chicks health-care feeds
Prescription: corn 55%, bean cake 38%, purple stem boneset extract 0.3%, calcium hydrogen phosphate 1.4%, stone powder 1%, Sal 0.3%, oil 3%, additive 1%.
Claims (10)
1. Eupatorium cannabinum L. (Eupatorium coelestinum L.) or purple stem boneset extract are in the veterinary medicine of preparation treatment respiratory system disease or the application in the preparation raising immunity medicine.
2. the application in the veterinary medicine of preparation treatment respiratory system disease of Eupatorium cannabinum L. (Eupatorium coelestinum L.) according to claim 1 or purple stem boneset extract, described treatment respiratory system disease comprises antiinflammatory, cough-relieving, eliminates the phlegm, treats influenza or infectious bronchitis.
3. application according to claim 1, described purple stem boneset extract is used to prepare oral formulations, and described oral formulations is capsule, powder or granule.
4. Eupatorium cannabinum L. (Eupatorium coelestinum L.) or its extract are as the application of additive for farm animal feed aspect.
5. Eupatorium cannabinum L. (Eupatorium coelestinum L.) according to claim 3 or its extract are as the application of additive for farm animal feed aspect, and described feed additive addition accounts for the 0.1-20% of animal daily ration.
6. Eupatorium cannabinum L. (Eupatorium coelestinum L.) according to claim 4 or its extract are as the application of additive for farm animal feed aspect, account for the 0.01-5% of animal daily ration as the addition of feed additive with purple stem boneset extract, account for the 0.5-20% of animal daily ration with Eupatorium cannabinum L. (Eupatorium coelestinum L.) as the addition of feed additive, described feedstuff is used for prevention or treatment respiratory system disease.
7. according to the arbitrary described application of claim 1-6, described Eupatorium cannabinum L. (Eupatorium coelestinum L.) is an Eupatorium cannabinum L. (Eupatorium coelestinum L.) plant shoot branch; Described purple stem boneset extract, preparation by the following method: raw material is the aerial parts of Eupatorium cannabinum L. (Eupatorium coelestinum L.), dries in the shade after gathering, pulverizing, extraction, merge extractive liquid,, concentrate drying, promptly gets purple stem boneset extract.
8. adopt the compound microorganism ferments detoxification before the application according to claim 7, described extraction.
9. application according to claim 7, described water or organic solvent or the water-containing organic solvent of being extracted as adopts lixiviate or supersound extraction or heating and refluxing extraction 1-3 time, and the 3-10 that each solvent volume consumption is a medical material weight is doubly; Described concentrate drying is that decompression and solvent recovery does not have alcohol flavor postlyophilization or spray drying to doing or being concentrated into.
10. application according to claim 7, described organic solvent is ethanol or methanol or the two mixture, described water-containing organic solvent is that the organic solvent volumetric concentration is the aqueous solution of 10-95%, described extraction time is 12-24h, its time of described supersound extraction is 10-30min, and its time of described heating and refluxing extraction is 1-3h.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103461731A (en) * | 2013-10-08 | 2013-12-25 | 贵州大学 | Crofton weed feed used for pigs and preparation method thereof |
| CN103880900A (en) * | 2014-03-06 | 2014-06-25 | 山东禾本堂生物科技有限公司 | Flavonoid glycoside compound as well as preparation method and application thereof |
| CN103961401A (en) * | 2013-03-18 | 2014-08-06 | 广西大学 | Compound medicine comprising herba lycopi and sulfamonomethoxine sodium and used for livestock and poultry |
| CN114272552A (en) * | 2021-12-06 | 2022-04-05 | 紫茎泽兰科技(云南)有限公司 | Method for detoxifying Eupatorium adenophorum |
| CN115474651A (en) * | 2022-09-19 | 2022-12-16 | 武汉新华扬生物股份有限公司 | Compound biological agent for eupatorium adenophorum spreng and application thereof |
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| CN101422481A (en) * | 2008-12-17 | 2009-05-06 | 四川大学 | Use of Eupatorium adenophorum polysaccharide for antibiosis or antivirus |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101422481A (en) * | 2008-12-17 | 2009-05-06 | 四川大学 | Use of Eupatorium adenophorum polysaccharide for antibiosis or antivirus |
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| CN103961401A (en) * | 2013-03-18 | 2014-08-06 | 广西大学 | Compound medicine comprising herba lycopi and sulfamonomethoxine sodium and used for livestock and poultry |
| CN103461731A (en) * | 2013-10-08 | 2013-12-25 | 贵州大学 | Crofton weed feed used for pigs and preparation method thereof |
| CN103461731B (en) * | 2013-10-08 | 2014-12-17 | 贵州大学 | Crofton weed feed used for pigs and preparation method thereof |
| CN103880900A (en) * | 2014-03-06 | 2014-06-25 | 山东禾本堂生物科技有限公司 | Flavonoid glycoside compound as well as preparation method and application thereof |
| CN103880900B (en) * | 2014-03-06 | 2016-03-30 | 山东禾本堂生物科技有限公司 | A kind of flavonoid glycoside compounds and its production and use |
| CN114272552A (en) * | 2021-12-06 | 2022-04-05 | 紫茎泽兰科技(云南)有限公司 | Method for detoxifying Eupatorium adenophorum |
| CN115474651A (en) * | 2022-09-19 | 2022-12-16 | 武汉新华扬生物股份有限公司 | Compound biological agent for eupatorium adenophorum spreng and application thereof |
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| Publication number | Publication date |
|---|---|
| CN102048785B (en) | 2012-10-31 |
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