CN102028003A - Brown rice planthopper insect repellent as well as preparation method and application thereof - Google Patents
Brown rice planthopper insect repellent as well as preparation method and application thereof Download PDFInfo
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- CN102028003A CN102028003A CN2010105952027A CN201010595202A CN102028003A CN 102028003 A CN102028003 A CN 102028003A CN 2010105952027 A CN2010105952027 A CN 2010105952027A CN 201010595202 A CN201010595202 A CN 201010595202A CN 102028003 A CN102028003 A CN 102028003A
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Abstract
The invention discloses a brown rice planthopper insect repellent as well as a preparation method and application thereof. The brown rice planthopper insect repellent comprises the following components by weight: 1-80mg of pepper extract, 1-80mg of water hyacinth extract and 0.01-0.10mg of farnesene which are dissolved in each ml of methanol solvent. According to the invention, the insect repellent prepared from the methanol extract of plants and a volatile matter preparation can repel the feeding of female brown rice planthopper adult insects and nymph and the laying of the female adult insets, thereby reducing the hazard and the population quantity of the brown rice planthopper insect. The insect repellent has the characteristics of low cost, high efficiency, environment friendliness, no harm to human beings and livestock and the like and broad industrial prospect. With the simple preparation method, the insect repellent is especially suitable for non-polluted production of rice and has broad economic, social and biological benefits.
Description
Technical field
The present invention relates to a kind of can effectively regulate and control the rice fulgorid adult lay eggs behavior with become, the repellant of nymph trophic behaviour, particularly relate to repellant that a kind of host's volatile matter that utilizes non-host's secondary metabolites and insect pest to induce regulates and control the behavior of laying eggs of rice fulgorid and its production and application.
Background technology
Rice fulgorid is one of most important insect on China paddy rice.Its main kind comprises brown planthopper Nilaparvata lugens
With white-backed planthopper Sogatella furcifera etc.In recent years, rice fulgorid broke out greatly every year, and China's rice yield has been caused heavy losses.Zhejiang Province Department of Agriculture survey data in 2005 shows, the local rice field in Zhejiang Province, the every 667m of the 5th generation brown planthopper generating capacity
2Reach 66.7 ten thousand hm that have more than 300,000
2, account for 86% of the total cultivated area of Zhejiang Province paddy rice; Every 667m
2The rice field area of rice fulgorid generating capacity above 1,000,000 has 33.3 ten thousand hm
2, account for 42% of the total cultivated area of Zhejiang Province paddy rice; The field piece rice fulgorid generating capacity of causing harm the most serious is up to every 667m
2More than 1,000 ten thousand; The area of total crop failure reaches 5333hm
2, cause direct rice yield to lose 1,200,000 tons.Current, the main chemical pesticide that relies on is controlled its harm in the production.Long-term a large amount of use chemical pesticide is killed natural enemies, contaminated environment not only, and causes pest resistance to insecticide and increase wildness again.Existent environment of people and health have been caused serious threat.Therefore, developing safely and effectively, environment friendly agricultural has become the urgent task that the control brown planthopper endangers.
Since the plant resource compound have to people and animals' low toxicity, in environment easy advantage such as degraded, therefore be an important source developing environment friendly agricultural.Up to now, although there are some researchs to report the repellent action of some plant extractss to brown planthopper, the action effect of plant extracts to a certain worm attitude of brown planthopper only reported in these researchs mostly, and to its resultant effect and unclear.If any discovering that the ginkgo leaf ethanol extract has stronger refusing to keep away activity to the brown planthopper nymph; Gelsemium elegan, red chinaberry and Fructus Bruceae extract have the deterrent effect of significantly laying eggs to brown planthopper.Clock waits the food refusal effect of 47 kinds of non-host plant ethanol extracts of 31 sections to the brown back rice plant hopper adult of having studied all one's life, and the extract of find garlic, neem oil, spending Africa mountain soya bean in vain is obvious to brown planthopper adult food refusal effect.The invention provides a kind of utilize plant resource compound configuration can get the lay eggs repellant of generation repellent action of food, female adult worm to brown planthopper nymph and female adult worm, this has important facilitation to the improvement that improves brown planthopper.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, a kind of brown planthopper repellant and its production and application is provided.
The objective of the invention is to be achieved through the following technical solutions: a kind of brown planthopper repellant, it comprises following components in weight percentage: dissolving pepper extract 1-80mg, water hyacinth extract 1-80mg and the rare 0.01-0.10mg of Fa Ni in every ml alcohol solvent.
Further, described brown planthopper repellant comprises following components in weight percentage: dissolving pepper extract 5-70mg, water hyacinth extract 5-70mg and the rare 0.02-0.08mg of Fa Ni in every ml alcohol solvent.
Further, described brown planthopper repellant comprises following components in weight percentage: dissolving pepper extract 10-60mg, water hyacinth extract 10-60mg and the rare 0.03-0.07mg of Fa Ni in every ml alcohol solvent.
The preparation method of above-mentioned brown planthopper repellant may further comprise the steps:
(1) preparation Chinese prickly ash ethanol extract: 65 ℃ of oven for drying, liquid nitrogen grinding is crossed 60 mesh sieves with Chinese prickly ash, is that 95% ethanol cold soaking extracts with percent by volume, and Chinese prickly ash is soaked 36h in 6 times of ethanol, and ultrasonic 1h gets filtrate after the filtration; Filter residue soaks 24h again in 3 times of ethanol, filter.Merge filtrate twice, be concentrated into driedly, preserve standby.
(2) preparation weter hyacinth ethanol extract: 65 ℃ of oven for drying, liquid nitrogen grinding is crossed 60 mesh sieves with weter hyacinth, is that 95% ethanol cold soaking extracts with percent by volume, and weter hyacinth is soaked 36h in 6 times of ethanol, and ultrasonic 1h gets filtrate after the filtration; Filter residue soaks 24h again in 3 times of ethanol, filter.Merge filtrate twice, be concentrated into driedly, preserve standby.
(3) preparation repellant: press each component of following formulated: dissolve the rare 0.01-0.10mg of dissolution method Buddhist nun in water hyacinth extract 1-80mg, the 1ml alcohol solvent in dissolving pepper extract 1-80mg, the 1ml alcohol solvent in the 1ml alcohol solvent, evenly mix, get the brown planthopper repellant;
Above-mentioned brown planthopper repellant is got food and the application of female adult worm aspect laying eggs repellent brown planthopper female adult worm, nymph.
The invention has the beneficial effects as follows that the present invention utilizes ethanol extract of plant and the repellant that volatile matter is made, can get food and the female adult worm generation repellent action of laying eggs to brown planthopper female adult worm, nymph, thereby reduce causing harm and population quantity of brown planthopper.Characteristics such as this repellant has that cost is low, efficient is high, environmentally friendly, person poultry safety; Brown planthopper repellant of the present invention has wide industrialization prospect, because the preparation method is simple, is particularly suitable for using in the nuisanceless production of paddy rice, has wide economy, society and ecological benefits.
Description of drawings
Fig. 1 gives birth to survey installation drawing;
Fig. 2 is the repellent effect figure that the Chinese prickly ash ethanol extract is got food and laid eggs the brown planthopper female adult worm;
Fig. 3 is the repellent effect figure of weter hyacinth ethanol extract to the brown planthopper nymph;
Fig. 4 is the repellent effect figure of the repellant of configuration to the brown planthopper imago/nymph;
The repellant of Fig. 5 configuration is to the repellent effect figure of field brown planthopper.
Embodiment
Brown planthopper repellant of the present invention comprises following components in weight percentage: dissolving pepper extract 1-80mg, water hyacinth extract 1-80mg and the rare 0.01-0.10mg of Fa Ni in every ml alcohol solvent.
Preferably, the brown planthopper repellant comprises following components in weight percentage: dissolving pepper extract 5-70mg, water hyacinth extract 5-70mg and the rare 0.02-0.08mg of Fa Ni in every ml alcohol solvent.
Best, the brown planthopper repellant comprises following components in weight percentage: dissolving pepper extract 10-60mg, water hyacinth extract 10-60mg and the rare 0.03-0.07mg of Fa Ni in every ml alcohol solvent.
The preparation method of brown planthopper repellant of the present invention may further comprise the steps:
1, preparation Chinese prickly ash ethanol extract: 65 ℃ of oven for drying, liquid nitrogen grinding is crossed 60 mesh sieves with Chinese prickly ash, is that 95% ethanol cold soaking extracts with percent by volume, and Chinese prickly ash is soaked 36h in 6 times of ethanol, and ultrasonic 1h gets filtrate after the filtration; Filter residue soaks 24h again in 3 times of ethanol, filter.Merge filtrate twice, be concentrated into driedly, preserve standby.
2, preparation weter hyacinth ethanol extract: 65 ℃ of oven for drying, liquid nitrogen grinding is crossed 60 mesh sieves with weter hyacinth, is that 95% ethanol cold soaking extracts with percent by volume, and weter hyacinth is soaked 36h in 6 times of ethanol, and ultrasonic 1h gets filtrate after the filtration; Filter residue soaks 24h again in 3 times of ethanol, filter.Merge filtrate twice, be concentrated into driedly, preserve standby.
3, preparation repellant: press each component of following formulated: dissolve the rare 0.01-0.10mg of dissolution method Buddhist nun in water hyacinth extract 1-80mg, the 1ml alcohol solvent in dissolving pepper extract 1-80mg, the 1ml alcohol solvent in the 1ml alcohol solvent, evenly mix, get the brown planthopper repellant;
Preferably, take by weighing each component of repellant by following prescription in the step (3): 1ml alcohol solvent dissolving pepper extract 5-70mg, 1ml alcohol solvent dissolving water hyacinth extract 5-70mg, the rare 0.02-0.08mg of 1ml alcohol solvent dissolution method Buddhist nun.
Best, take by weighing each component of repellant by following prescription in the step (3): 1ml alcohol solvent dissolving pepper extract 10-60mg, 1ml alcohol solvent dissolving water hyacinth extract 10-60mg, the rare 0.03-0.07mg of 1ml alcohol solvent dissolution method Buddhist nun.
Plant extracts of the present invention all derives from nature, and extraction step is simple, and the organic solvent usage amount is little, to operating personnel and Environmental security.
The rare product that can adopt SIGMA company of method Buddhist nun, but be not limited thereto.
Repellant of the present invention is made up of 3 kinds of different materials of the mechanism of action, and insect is difficult to produce resistance.Brown planthopper repellant reasonable mixture ratio, effectively repellent brown planthopper female adult worm, nymph get the food and female adult worm lay eggs.Brown planthopper repellant of the present invention has been given full play to natural plants ethanol extract and volatile matter effect characteristics separately, is a kind of repellant of brown planthopper safely and effectively.
Further specify the present invention below in conjunction with specific embodiment.
The preparation (weight portion) of embodiment 1 brown planthopper repellant
1 part of preparation Chinese prickly ash ethanol extract, 1 part of weter hyacinth ethanol extract, rare 0.01 part of method Buddhist nun mix and promptly make the brown planthopper repellant.Experiment showed, this brown planthopper repellant effectively repellent brown planthopper female adult worm, nymph get the food and female adult worm lay eggs.
Embodiment 2: the preparation of brown planthopper repellant (weight portion)
80 parts of preparation Chinese prickly ash ethanol extracts, 80 parts of weter hyacinth ethanol extracts, rare 0.10 part of method Buddhist nun mix and promptly make the brown planthopper repellant.Experiment showed, this brown planthopper repellant effectively repellent brown planthopper female adult worm, nymph get the food and female adult worm lay eggs.
Embodiment 3: the preparation of brown planthopper repellant (weight portion)
5 parts of preparation Chinese prickly ash ethanol extracts, 5 parts of weter hyacinth ethanol extracts, rare 0.02 part of method Buddhist nun mix and promptly make the brown planthopper repellant.Experiment showed, this brown planthopper repellant effectively repellent brown planthopper female adult worm, nymph get the food and female adult worm lay eggs.
Embodiment 4: the preparation of brown planthopper repellant (weight portion)
70 parts of preparation Chinese prickly ash ethanol extracts, 70 parts of weter hyacinth ethanol extracts, rare 0.08 part of method Buddhist nun mix and promptly make the brown planthopper repellant.Experiment showed, this brown planthopper repellant effectively repellent brown planthopper female adult worm, nymph get the food and female adult worm lay eggs.
Embodiment 5: the preparation of brown planthopper repellant (weight portion)
10 parts of preparation Chinese prickly ash ethanol extracts, 10 parts of weter hyacinth ethanol extracts, rare 0.03 part of method Buddhist nun mix and promptly make the brown planthopper repellant.Experiment showed, this brown planthopper repellant effectively repellent brown planthopper female adult worm, nymph get the food and female adult worm lay eggs.
Embodiment 6: the preparation of brown planthopper repellant (weight portion)
40 parts of preparation Chinese prickly ash ethanol extracts, 40 parts of weter hyacinth ethanol extracts, rare 0.05 part of method Buddhist nun mix and promptly make the brown planthopper repellant.Experiment showed, this brown planthopper repellant effectively repellent brown planthopper female adult worm, nymph get the food and female adult worm lay eggs.
Embodiment 7: the preparation of brown planthopper repellant
1, the preparation of Chinese prickly ash ethanol extract: the Chinese prickly ash that collects is put into 65 ℃ of oven for drying, and liquid nitrogen grinding is crossed 60 mesh sieves, adopts 95% ethanol cold soaking extraction of substance method, and Chinese prickly ash is soaked 36h in 6 times of ethanol, and ultrasonic 1h gets filtrate after the filtration; Then filter residue is soaked 24h again in 3 times of ethanol, filter, merge filtrate twice, be concentrated into driedly, preserve standby.
2, the preparation of weter hyacinth ethanol extract: the weter hyacinth that collects is put into 65 ℃ of oven for drying, and liquid nitrogen grinding is crossed 60 mesh sieves, adopts 95% ethanol cold soaking extraction of substance method, and weter hyacinth is soaked 36h in 6 times of ethanol, and ultrasonic 1h gets filtrate after the filtration; Then filter residue is soaked 24h again in 3 times of ethanol, filter, merge filtrate twice, be concentrated into driedly, preserve standby.
3, the preparation of repellant: press each component of following formulated: dissolving pepper extract 1mg, water hyacinth extract 1mg, the rare 0.01mg of method Buddhist nun in the 1ml alcohol solvent, evenly mix then, promptly get the brown planthopper repellant.
Embodiment 8
The brown planthopper repellant that adopts embodiment 1 to make.Get the rice seedling that two strains are in the Sheng phase of tillering, plant in the plastic cup of tool mill water culture nutrient solution, and at the narrow meshed glass tube of stem stalk position outer cover of two seedlings (diameter 40mm, on 24 apertures are arranged), (as Fig. 1) sealed with sponge in two ends.In the two strain paddy rice seedlings, the stem stalk of a strain is smeared the ethanol extract of 30 μ l plants with writing brush, and another strain is smeared 95% ethanol and compared.After treating 30min, in cloche, connect 15 3-4 nymphs in age, place a temperature control (25 ± 1 ℃), control light (L: D=16: 8) in the greenhouse, observe and write down the quantity that each rice straw stops nymph behind the 24h then.Calculate the land rate of nymph on rice seedling.Repeat 10 times.
Survey data from Fig. 4 as can be seen, the quantity of brown planthopper nymph in contrast rice strain has tangible repellent effect apparently higher than handling in the rice strain.The brown planthopper repellant that adopts embodiment 2～6 to make carries out experiment same as described above, also can reach the technique effect similar to embodiment 1.
Embodiment 9
The brown planthopper repellant that adopts embodiment 1 to make.Get the rice seedling that two strains are in the Sheng phase of tillering, plant in the plastic cup of tool mill water culture nutrient solution, and at the narrow meshed glass tube of stem stalk position outer cover of two seedlings (diameter 40mm, on 24 apertures are arranged), (as Fig. 1) sealed with sponge in two ends.In the two strain paddy rice seedlings, the stem stalk of a strain is smeared the ethanol extract of 30 μ l plants with writing brush, and another strain is smeared 95% ethanol and compared.After treating 30min, in cloche, connect 15 head length wing type female adult worm, place a temperature control (25 ± 1 ℃), control light (L: D=16: 8) in the greenhouse then, 24h observes and writes down the quantity that each rice straw stops female adult worm, take out the rice stem behind the 48h, under stereomicroscope, cut open and look into, and each ovum grain number of handling of record.Repeat 10 times.
Survey data from Fig. 4 as can be seen, brown planthopper female adult worm quantity and ovum amount in contrast rice strain will be apparently higher than handling in the rice strain, female adult worm got food and lay eggs have tangible repellent effect.The brown planthopper repellant that adopts embodiment 2～6 to make carries out test same as described above, also can reach the technique effect similar to embodiment 1.
Embodiment 10
The brown planthopper repellant that adopts embodiment 1 to make.Get the close 9m of two block length gesture
2Paddy field, 1m paddy rice band is isolated all around, before the dispenser two plots of paddy fields are investigated the brown planthopper quantity, after the 3h, one of them field piece sprays the 700ml repellant with small-sized watering can, another field piece sprays the 700ml contrast, and the quantity of brown planthopper adult and nymph respectively handled in investigation in 2 days, 4 days, 10 days and record behind medicine respectively.Repeat 3 times.
Survey data from Fig. 5 as can be seen, the brown planthopper quantity in the control plot is apparently higher than handling in the sub-district, especially after dispenser 10 days, brown planthopper had tangible repellent effect.The brown planthopper repellant that adopts embodiment 2～6 to make carries out test same as described above, also can reach the technique effect similar to embodiment 1.
The foregoing description is used for the present invention that explains, rather than limits the invention, and in the protection domain of spirit of the present invention and claim, any modification and change to the present invention makes all fall into protection scope of the present invention.
Claims (5)
1. a brown planthopper repellant is characterized in that it comprises following components in weight percentage: dissolving pepper extract 1-80mg, water hyacinth extract 1-80mg and the rare 0.01-0.10mg of Fa Ni in every ml alcohol solvent.
2. according to the described brown planthopper repellant of claim 1, it is characterized in that described brown planthopper repellant comprises following components in weight percentage: dissolving pepper extract 5-70mg, water hyacinth extract 5-70mg and the rare 0.02-0.08mg of Fa Ni in every ml alcohol solvent.
3. according to the described brown planthopper repellant of claim 1, it is characterized in that described brown planthopper repellant comprises following components in weight percentage: dissolving pepper extract 10-60mg, water hyacinth extract 10-60mg and the rare 0.03-0.07mg of Fa Ni in every ml alcohol solvent.
4. the preparation method of the described brown planthopper repellant of claim 1 is characterized in that, may further comprise the steps:
(1) preparation Chinese prickly ash ethanol extract: 65 ℃ of oven for drying, liquid nitrogen grinding is crossed 60 mesh sieves with Chinese prickly ash, is that 95% ethanol cold soaking extracts with percent by volume, and Chinese prickly ash is soaked 36h in 6 times of ethanol, and ultrasonic 1h gets filtrate after the filtration; Filter residue soaks 24h again in 3 times of ethanol, filter.Merge filtrate twice, be concentrated into driedly, preserve standby.
(2) preparation weter hyacinth ethanol extract: 65 ℃ of oven for drying, liquid nitrogen grinding is crossed 60 mesh sieves with weter hyacinth, is that 95% ethanol cold soaking extracts with percent by volume, and weter hyacinth is soaked 36h in 6 times of ethanol, and ultrasonic 1h gets filtrate after the filtration; Filter residue soaks 24h again in 3 times of ethanol, filter.Merge filtrate twice, be concentrated into driedly, preserve standby.
(3) preparation repellant: press each component of following formulated: dissolve the rare 0.01-0.10mg of dissolution method Buddhist nun in water hyacinth extract 1-80mg, the 1ml alcohol solvent in dissolving pepper extract 1-80mg, the 1ml alcohol solvent in the 1ml alcohol solvent, evenly mix, get the brown planthopper repellant.
5. the described brown planthopper repellant of claim 1 is got the application aspect laying eggs of food and female adult worm repellent brown planthopper female adult worm, nymph.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010105952027A CN102028003B (en) | 2010-12-10 | 2010-12-10 | Brown rice planthopper insect repellent as well as preparation method and application thereof |
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| CN2010105952027A CN102028003B (en) | 2010-12-10 | 2010-12-10 | Brown rice planthopper insect repellent as well as preparation method and application thereof |
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| CN102028003A true CN102028003A (en) | 2011-04-27 |
| CN102028003B CN102028003B (en) | 2013-06-19 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102771520A (en) * | 2012-07-11 | 2012-11-14 | 广西山云生化科技有限公司 | Plant yield increasing agent, preparation method thereof and applications in increasing yield of vegetable plants |
| CN105010422A (en) * | 2015-04-30 | 2015-11-04 | 新昌县大成生物科技有限公司 | Botanical rice planthopper repellent |
| CN106035415A (en) * | 2016-06-15 | 2016-10-26 | 安徽菲扬农业科技有限公司 | Pest repellent and preparation method thereof |
| CN106135317A (en) * | 2016-06-30 | 2016-11-23 | 安徽金培因科技有限公司 | A kind of insects repellant and preparation method thereof |
| CN108207949A (en) * | 2018-02-01 | 2018-06-29 | 江西省农业科学院农业应用微生物研究所（江西省农村能源研究中心） | A kind of application of alcohol compound in terms of brown paddy plant hopper is prevented |
-
2010
- 2010-12-10 CN CN2010105952027A patent/CN102028003B/en not_active Expired - Fee Related
Non-Patent Citations (2)
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| 苏远萍,等: "13种植物乙醇提取物对褐飞虱的生物活性研究", 《中国农学通报》 * |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102771520A (en) * | 2012-07-11 | 2012-11-14 | 广西山云生化科技有限公司 | Plant yield increasing agent, preparation method thereof and applications in increasing yield of vegetable plants |
| CN105010422A (en) * | 2015-04-30 | 2015-11-04 | 新昌县大成生物科技有限公司 | Botanical rice planthopper repellent |
| CN106035415A (en) * | 2016-06-15 | 2016-10-26 | 安徽菲扬农业科技有限公司 | Pest repellent and preparation method thereof |
| CN106135317A (en) * | 2016-06-30 | 2016-11-23 | 安徽金培因科技有限公司 | A kind of insects repellant and preparation method thereof |
| CN108207949A (en) * | 2018-02-01 | 2018-06-29 | 江西省农业科学院农业应用微生物研究所（江西省农村能源研究中心） | A kind of application of alcohol compound in terms of brown paddy plant hopper is prevented |
| CN108207949B (en) * | 2018-02-01 | 2020-08-04 | 江西省农业科学院农业应用微生物研究所（江西省农村能源研究中心） | Application of alcohol compound in controlling brown planthopper |
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| CN102028003B (en) | 2013-06-19 |
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