CN102000145A - Traditional Chinese medicine and western medicine compound infusion liquid for preventing and treating endometritis of dam and preparing method thereof - Google Patents
Traditional Chinese medicine and western medicine compound infusion liquid for preventing and treating endometritis of dam and preparing method thereof Download PDFInfo
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Abstract
The present invention discloses a traditional Chinese medicine and western medicine compound infusion liquid for preventing and treating endometritis of dam and preparing method thereof, wherein the contents of various constituents are as follows: 0.5-5g/L of safflower extract, 1-10g/L of epimedium extract, 1-10g/L of leonurus extract, 5-50g/L of Maquinox, 10-100g/L of salicylic acid, 4-40g/L of Ciprofloxacin Lactate, 3-30g/L of metronidazole, 1-10g/L of borneol, 20-40g/L of polyvinylpyrrolidone, 100-200g/L of ethanol and 5-15g/L of tween-80, the rests are water for injection. In the present invention, the effective combination of traditional Chinese medicine and western medicine overcomes the shortages of big dosage, high dosing strength, becoming effective slowly and long course of treatment in pure traditional Chinese treatment, and the shortages of big side-effects and easily generated resistance in pure western medicine treatment. Symptoms and root causes are all treated, and the organic combination of antibacterial inflammation of antibiotics and repairing uterine function by traditional Chinese medicine ingredients is realized.
Description
Technical field
The invention belongs to field of veterinary, relate to a kind of Chinese medicine and western medicine perfusate of female animal endometritis and preparation method thereof that is used to prevent and treat.
Technical background
Endometritis is one of principal disease in the female livestock breed process, and ill female animal causes the sexual cycle disorder, and directly influences the vigor and the embryo nidation of sperm because the uterus is subjected to the influence of factors such as inflammation, inflammatory products and bacteriotoxin, causes joining repeatly infertile.If primary disease is treated untimely or the treatment improper measures, then can influence the fertility performance of female animal greatly, cause serious economy loss to animal husbandry.The sickness rate of female animal endometritis is very high, and is particularly huge to the harm of milch cow and large-scale pig farm sow.It is reported that the sickness rate of external milch cow accounts for 90% of infertile milch cow 30~40%.And the sickness rate of domestic cow endometritis is about 20~50%, accounts for 60~90% of infertile milch cow.Therefore the milch cow that is eliminated accounts for 10% of morbidity cattle.And sow trouble endometritis often causes conception rate not high, and breeding potential is low, and stillborn fetus is many, and puerperal, sow appetite was bad, few milk, and many diseases such as HUANGBAI(sic) dysentery take place in piglets.If after developing into the intractable endometritis, also can increase and produce the sow mortality.According to investigations, in large scale of pig farm was produced, endometritis often took place in sow (comprising multiparity sow and replacement gilt), and sickness rate is about 20%, and the pig farm that has is up to 40%.The sow of eliminating because of endometritis accounts for superseded more than 60% of sow of producing.
To the control of endometritis, at present based on antibacterials and hormonotherapy.Though these methods have certain curative effect, the heavy dose of use, even the endometritis recovery from illness, the female livestock breed performance also can reduce.The toxic and side effects of hormone is big in addition, and carcinogenic, teratogenesis and mutagenic action clinically lie dormant.Some medicine also can be residual in breast, meat, is detrimental to health, and the food-safety problem that brings has thus caused people's common concern.So advantages such as Chinese medicine is low with toxicity, noresidue, difficult generation drug resistance are used to the control of endometritis gradually.Chinese medicine is the main pathomechanism that takes place at endometritis, by strengthening uterus movement to promote the lochia discharge and to improve the uterus local blood circulation, regulate the breeding of immunity of organisms and rejection condition pathogenic bacterium to remove pathogen, the metabolism of regulon intrauterine tissue is to recover the uterus function.But the common dosage of pure Chinese prescription is big, and administration intensity height takes effect slowly, and the course of treatment is long.
In sum, preferably adopt the bonded method in Chinese and Western to realize treating both the principal and secondary aspects of a disease for the control of female animal endometritis.By the uterus perfusion, realize the combination of antibiotic anti-inflammation and Chinese medicine ingredients reparation uterus function.
Summary of the invention
The object of the present invention is to provide a kind of Chinese medicine and western medicine perfusate of preventing and treating the female animal endometritis.
Another object of the present invention is to provide a kind of preparation method of preventing and treating the Chinese medicine and western medicine perfusate of female animal endometritis.
Purpose of the present invention is achieved through the following technical solutions:
A kind of Chinese medicine and western medicine perfusate of preventing and treating the female animal endometritis, each components contents is in this infusion liquid: Flos Carthami extract 0.5~5g/L, Herba Epimedii extract 1~10g/L, Herba Leonuri extract 1~10g/L, LIJUNJING 5~50g/L, salicylic acid 10~100g/L, ciprofloxacin lactate 4~40g/L, metronidazole 3~30g/L, Borneolum Syntheticum 1~10g/L, polyvinylpyrrolidone 20~40g/L, ethanol 100~200g/L, tween 80 5~15g/L, surplus is a water for injection.Be preferably: Flos Carthami extract 2.5g/L, Herba Epimedii extract 5g/L, Herba Leonuri extract 5g/L, LIJUNJING 25g/L, salicylic acid 50g/L, ciprofloxacin lactate 20g/L, metronidazole 5g/L, Borneolum Syntheticum 5g/L, polyvinylpyrrolidone 30g/L, ethanol 150g/L, tween 80 10g/L, surplus is a water for injection.
The preparation method of the Chinese medicine and western medicine perfusate of above-mentioned control female animal endometritis, it is: incite somebody to action the Flos Carthami extract of weighing in proportion respectively with water for injection earlier, Herba Epimedii extract, Herba Leonuri extract, LIJUNJING, salicylic acid, ciprofloxacin lactate, join mixing in the proportion container after the metronidazole dissolving, during dissolving, earlier the water for injection preheating can be improved dissolution velocity, weighing polyvinylpyrrolidone in proportion, Borneolum Syntheticum, ethanol, tween 80 joins mixing in the proportion container, surplus adds the injection water and supplies, regulate pH value to 5~7, filter, embedding, sterilization, packing.
Described Flos Carthami extract, Herba Epimedii extract or Herba Leonuri extract ratio extract for adopting conventional method to extract, wherein the withdrawal ratio of each extract (mass ratio of former Chinese crude drug and extract) is: Flos Carthami extract: 10: 1 (promptly 10 kilograms of Flos Carthamis through extraction 1 kilogram of Flos Carthami extract); Herba Epimedii extract: 25: 1: Herba Leonuri extract: 20: 1.The said extracted thing can directly be bought from market, also can extract voluntarily.
The extracting method of extract is: with Chinese medicine through pulverizing several times, reach the micropowder state after, put into purified water and carry out ultrasonic extraction, get this extracting solution of Chinese medicine through the centrifugal treating after-filtration then, medicinal residues carry out extraction second time, extract so repeatedly 3 times.Discard medicinal residues, merge three times extracting solution.Add ethanol in extracting solution, the ethanol volume accounts for the 30%-70% of extracting solution and ethanol volume sum, adds flocculating agent then and precipitates 24~48 hours, and the starch of Chinese medicine extraction liquid the inside and the abundant post precipitation high speed centrifugation of colloid are filtered.Discard precipitate, keep filtrate, ethanol (these several Chinese medicines all contain volatile oil component, can not reclaim at normal temperatures and drying) is returned and receives in the cold drying of filtrate process negative pressure of vacuum, gets extract.
The present invention adopts the bonded mode of Chinese medicine and western medicine to make, and has the effect of antiinflammatory progestogenic.Wherein ciprofloxacin lactate, LIJUNJING (having another name called: Mequindox or 3-methyl-2-acetyl group quinoxaline-1,4-dioxide) have broad-spectrum bactericidal action, and sterilizing power is strong, and effect rapidly.Except that gram-negative bacteria and gram positive bacteria, bacillus pyocyaneus, part anaerobe also there is stronger antibacterial action.Metronidazole has powerful killing action to most of obligate anaerobes, and most antibacterials have stronger inhibitory action; The Flos Carthami blood circulation promoting and blood stasis dispelling promotes the discharge of intrauterine inflammatory products, improves blood circulation, regulates the uterus metabolism.Herba Leonuri has excited uterus, strengthens the effect of uterus muscle segment rule property contraction.In addition, Flos Carthami, Herba Leonuri etc. contains estrogen-like product, can promote cervical orifice open, for prerequisite is created in the discharge of intrauterine pathologic product.Borneolum Syntheticum has heat-clearing and fire-dispersing, the effect of reducing swelling and alleviating pain.Herba Epimedii has significant cellular immunization and humoral immunization facilitation simultaneously, energy human body immunity improving power; Polyvinylpyrrolidone has parcel, slow release and to endometrial adhesive attraction, drug distribution is fully acted on lastingly.
Beneficial effect of the present invention:
Chinese and western medicinal composition infusion liquid determined curative effect of the present invention, instant effect, scientific formula is remarkable to curative effect of disease such as bacillary endometritis.Chinese medicine ingredients of the present invention is a Chinese medicine extract, and its active constituent content is higher, has improved curative effect of medication.Compare with other administering modes, this medicine pours into by the uterus, and effect is direct, convenient drug administration, and dosage is little.
Infusion liquid of the present invention adopts the Chinese medicine and western medicine combination, made up the advantage of Chinese medicine and western medicine, effectively reduce simultaneously the deficiency of pure treatment by Chinese herbs or pure western medicine again, Chinese medicine component in the prescription has advantages such as toxicity is low, noresidue, difficult generation drug resistance, western medicine component and Chinese medicinal components partner treatment instant effect can effectively shorten the treatment course of treatment and reduce dosage.The present invention by the effective combination with Chinese medicine and Western medicine remedied that pure treatment by Chinese herbs dosage is big, administration intensity height, take effect slowly, the course of treatment is long, reach that pure western medicine side effect is big, cost is high, easily produce the deficiency of resistance.Realize the combination for the treatment of both the principal and secondary aspects of a disease and antibiotic anti-inflammation and Chinese medicine ingredients reparation uterus function.
The specific embodiment
Embodiment 1
With the water for injection that is preheated to about 70 ℃, it is (commercially available to dissolve Flos Carthami extract respectively, withdrawal ratio is: 10: 1, manufacturer: 2.5kg ShenFang,SiChuan letter ring crude drug company limited), Herba Epimedii extract is (commercially available, withdrawal ratio is: 25: 1, manufacturer: 5kg ShenFang,SiChuan letter ring crude drug company limited), Herba Leonuri extract is (commercially available, withdrawal ratio is: 20: 1, manufacturer: 5kg ShenFang,SiChuan letter ring crude drug company limited), LIJUNJING 25kg, salicylic acid 50kg, ciprofloxacin lactate 20kg, metronidazole 5kg, pour solution in material-compound tank mixing, in material-compound tank, add polyvinylpyrrolidone 30kg stirring and evenly mixing then, add the ethanol 150kg that is preheated to about 50 ℃, Borneolum Syntheticum 5kg stirring and dissolving mixing, add tween 80 10kg again, add the injection water and complement to 1000L and stir, regulate pH to 5~7 with lactic acid, after the assay was approved, filter, embedding, 105 ℃ of sterilization 30min packings promptly.
Embodiment 2
With the water for injection that is preheated to about 70 ℃, it is (commercially available to dissolve Flos Carthami extract respectively, withdrawal ratio is: 10: 1, manufacturer: 5kg ShenFang,SiChuan letter ring crude drug company limited), Herba Epimedii extract is (commercially available, withdrawal ratio is: 25: 1, manufacturer: 6kg ShenFang,SiChuan letter ring crude drug company limited), Herba Leonuri extract is (commercially available, withdrawal ratio is: 20: 1, manufacturer: 6kg ShenFang,SiChuan letter ring crude drug company limited), LIJUNJING 20kg, salicylic acid 40kg, ciprofloxacin lactate 25kg, metronidazole 10kg, pour solution in material-compound tank mixing, in material-compound tank, add polyvinylpyrrolidone 25kg stirring and evenly mixing then, add the ethanol 120kg that is preheated to about 50 ℃, Borneolum Syntheticum 6kg stirring and dissolving mixing, add tween 80 12kg again, add the injection water and complement to 1000L and stir, regulate pH to 5~7 with lactic acid, after the assay was approved, filter, embedding, 105 ℃ of sterilization 30min packings promptly.
Embodiment 3
With the water for injection that is preheated to about 70 ℃, it is (commercially available to dissolve Flos Carthami extract respectively, withdrawal ratio is: 10: 1, manufacturer: 1kg ShenFang,SiChuan letter ring crude drug company limited), Herba Epimedii extract is (commercially available, withdrawal ratio is: 25: 1, manufacturer: 9kg ShenFang,SiChuan letter ring crude drug company limited), Herba Leonuri extract is (commercially available, withdrawal ratio is: 20: 1, manufacturer: 9kg ShenFang,SiChuan letter ring crude drug company limited), LIJUNJING 10kg, salicylic acid 60kg, ciprofloxacin lactate 30kg, metronidazole 10kg, pour solution in material-compound tank mixing, in material-compound tank, add polyvinylpyrrolidone 20kg stirring and evenly mixing then, add the ethanol 100kg that is preheated to about 50 ℃, Borneolum Syntheticum 8kg stirring and dissolving mixing, add tween 80 5kg again, add the injection water and complement to 1000L and stir, regulate pH to 5~7 with lactic acid, after the assay was approved, filter, embedding, 105 ℃ of sterilization 30min packings promptly.
Embodiment 4 treatment sow endometritis tests
Test method: the multiparity broad sow that is selected from Zhejiang Hangzhou beacon kind pig farm 3~5 parity, by tame livestock production science diagnostic criteria, uterus secretions is mucus or purulence, and lists test in 20 sick pigs of tangible General Symptoms (fervescence is searched for food and reduced or stop).
Medication: behind 0.1% bromo geramine solution cleaning pudendum, use through the disinfectant deferent duct infusion liquid is imported intrauterine.Observed and record treatment situation in second day after the medication.
Tell 10 at random and be experimental group, to the new infusion liquid medicinal liquid of intrauterine perfusion 100ml embodiment 1 preparation, the next day, pour into once more at every turn, and severe patient can pour into 3 times.
10 matched groups, wherein 5 is that infusion liquid (the Chinese medicinal perfusion liquid of the patent No.: the 200310106276.X) 15ml of main effective ingredient does contrast with pure Chinese medicine, the next day, pour into once, until rehabilitation, because the concentration of effective ingredient is bigger in the pure Chinese medicinal perfusion liquid as a comparison, thus when treatment chemical liquid consumption less relatively; Middle pharmaceutically active ingredient concentration in the used Chinese medicine and western medicine perfusate of experimental group is less relatively, and chemical liquid consumption is bigger during treatment, and the consumption of the middle pharmaceutically active ingredient that uses during the two treatment is suitable.Other 5 are adopted western medicine method commonly used to treat: 0.1% potassium permanganate solution flushing uterus, perfusion penicillin 4,000,000 IU, streptomycin 2,000,000 IU, every flushing perfusion in two days once, every day twice intramuscular injection penicillin, streptomycin 8,000,000 IU five days.Day by day the variation that observes the symptoms is according to the recovery situation medication.Compare rehabilitation duration length.And after the record rehabilitation to the interval of oestrusing, observe and whether to be impregnated.
Result of the test: body temperature is between 38.9~40.7 ℃ before 20 sick pig treatments of endometritis, and appetite reduces or do not eat; Wherein 13 secretions are mucus, and 7 is purulence.Symptom obviously alleviated after 10 of experimental grouies poured into once with this product, and body temperature drops to 38.4~39.6 ℃, and sick pig feed desire begins to recover, and uterus secretions begins to reduce, and character is improved.After twice of the medication, 8 sick pig rehabilitations, appetite is normal, 3~4 days courses of treatment.Remain the rehabilitation after medication 3 times of 2 severe patients, 6 days courses of treatment.14 sick pig breedings of all oestrusing in 17~42 days after rehabilitation are impregnated for 9.Matched group, rehabilitation after twice of 1 medication of the sick pig back rehabilitations of 5 perfusion Chinese medicines, 4 medications three times, and all oestrus and be impregnated.5 contrast 3 rehabilitations in the 6th day of disease pig with Western medicine, and 2 rehabilitations in the 7th day are all oestrused, and wherein are impregnated for 3.From therapeutic outcome, the Chinese medicine and western medicine compound filling liquor ratio pure Chinese medicinal preparation treatment shortening course of treatment, higher than shortening of Western medicine infusion liquid inter oestrual period and conception rate.
The curative effect of table 1 infusion liquid treatment sow endometritis
Clinical verification:
This product through test of pesticide effectiveness checking back priority in Shandong, ground such as Jiangsu, Zhejiang and Guangdong apply, and are subjected to numerous raisers' favorable comment.According to the feedback data statistics (partial results sees Table 2) of some areas 04 month in November, 2010 in 2009, endometritis 513 examples for the treatment of the multiparity female animal with this infusion liquid, milch cow 127 examples wherein, sow 386 examples.The cure rate of cattle is more than 86%, and the cure rate of pig is more than 90%.
Table 2.2009 year 04 month to part market feedback in November, 2010 statistics table
Claims (5)
1. Chinese medicine and western medicine perfusate of preventing and treating the female animal endometritis, it is characterized in that each components contents is in this infusion liquid: Flos Carthami extract 0.5~5g/L, Herba Epimedii extract 1~10g/L, Herba Leonuri extract 1~10g/L, LIJUNJING 5~50g/L, salicylic acid 10~100g/L, ciprofloxacin lactate 4~40g/L, metronidazole 3~30g/L, Borneolum Syntheticum 1~10g/L, polyvinylpyrrolidone 20~40g/L, ethanol 100~200g/L, tween 80 5~15g/L, surplus is a water for injection.
2. the Chinese medicine and western medicine perfusate of control female animal endometritis according to claim 1, it is characterized in that each components contents is in this infusion liquid: Flos Carthami extract 2.5g/L, Herba Epimedii extract 5g/L, Herba Leonuri extract 5g/L, LIJUNJING 25g/L, salicylic acid 50g/L, ciprofloxacin lactate 20g/L, metronidazole 5g/L, Borneolum Syntheticum 5g/L, polyvinylpyrrolidone 30g/L, ethanol 150g/L, tween 80 10g/L, surplus is a water for injection.
3. the Chinese medicine and western medicine perfusate of control female animal endometritis according to claim 1 and 2 is characterized in that the withdrawal ratio of described Flos Carthami extract, Herba Epimedii extract, Herba Leonuri extract is: Flos Carthami extract 10: 1, Herba Epimedii extract 25: 1, Herba Leonuri extract 20: 1.
4. the preparation method of the Chinese medicine and western medicine perfusate of the described control of claim 1 female animal endometritis, it is characterized in that: join mixing in the proportion container after the Flos Carthami extract of weighing, Herba Epimedii extract, Herba Leonuri extract, LIJUNJING, salicylic acid, ciprofloxacin lactate, the metronidazole dissolving in proportion respectively with water for injection earlier, weighing polyvinylpyrrolidone, Borneolum Syntheticum, ethanol, tween 80 join mixing in the proportion container in proportion, surplus adds the injection water and supplies, regulate pH value to 5~7, filtration, embedding, sterilization, packing.
5. the preparation method of the Chinese medicine and western medicine perfusate of control female animal endometritis according to claim 4 is characterized in that the withdrawal ratio of described Flos Carthami extract, Herba Epimedii extract, Herba Leonuri extract is: Flos Carthami extract 10: 1, Herba Epimedii extract 25: 1, Herba Leonuri extract 20: 1.
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Cited By (6)
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CN102552366A (en) * | 2012-02-14 | 2012-07-11 | 江西正邦动物保健品有限公司 | Chinese medicinal extract composition as female livestock uterus cleaning liquid |
CN103768603A (en) * | 2014-02-25 | 2014-05-07 | 刘明洋 | Uterine perfusion fluid for livestock delivery |
CN104208142A (en) * | 2013-05-29 | 2014-12-17 | 镇江威特药业有限责任公司 | Perfusate for preventing and treating infertility of female animals, and its preparation method |
CN104840502A (en) * | 2015-01-09 | 2015-08-19 | 广西南宁市桃源兽药厂 | Composition containing epimedium herb and mequindox and used for livestock and poultry, and preparation method thereof |
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CN1813738A (en) * | 2005-12-14 | 2006-08-09 | 刘满秋 | External-use medicament for postpartum uterus-cleaning for sow |
CN101238849A (en) * | 2007-02-09 | 2008-08-13 | 陈高芹 | Female livestock postpartum feed additive and preparation thereof |
CN101879240A (en) * | 2010-07-20 | 2010-11-10 | 宁夏大学 | Chinese medicinal compound suppository for treating endometritis of livestock and preparation method thereof |
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CN102552366A (en) * | 2012-02-14 | 2012-07-11 | 江西正邦动物保健品有限公司 | Chinese medicinal extract composition as female livestock uterus cleaning liquid |
CN104208142A (en) * | 2013-05-29 | 2014-12-17 | 镇江威特药业有限责任公司 | Perfusate for preventing and treating infertility of female animals, and its preparation method |
CN103768603A (en) * | 2014-02-25 | 2014-05-07 | 刘明洋 | Uterine perfusion fluid for livestock delivery |
CN104840502A (en) * | 2015-01-09 | 2015-08-19 | 广西南宁市桃源兽药厂 | Composition containing epimedium herb and mequindox and used for livestock and poultry, and preparation method thereof |
CN106106364A (en) * | 2016-07-29 | 2016-11-16 | 董标 | A kind of cultural method improving sow reproductive performance |
CN106106364B (en) * | 2016-07-29 | 2019-03-08 | 深圳农畉农产品有限公司 | A kind of cultural method improving sow reproductive performance |
CN113144006A (en) * | 2021-04-27 | 2021-07-23 | 湖南美可达生物资源股份有限公司 | Traditional Chinese medicine composition for treating endometritis of female animals and preparation method and application thereof |
CN113144006B (en) * | 2021-04-27 | 2022-04-08 | 湖南美可达生物资源股份有限公司 | Traditional Chinese medicine composition for treating endometritis of female animals and preparation method and application thereof |
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