CN101953440B - House fly larva feed containing cellulomonas flavigena - Google Patents
House fly larva feed containing cellulomonas flavigena Download PDFInfo
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- CN101953440B CN101953440B CN2010105033107A CN201010503310A CN101953440B CN 101953440 B CN101953440 B CN 101953440B CN 2010105033107 A CN2010105033107 A CN 2010105033107A CN 201010503310 A CN201010503310 A CN 201010503310A CN 101953440 B CN101953440 B CN 101953440B
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Abstract
The invention discloses a house fly larva feed containing cellulomonas flavigena. Based on 100 mass percent, the house fly larva feed consists of 0.6 to 65 percent of cellulomonas flavigena liquid and 35 to 99.4 percent of common house fly larva feed components; and the cellulomonas flavigena liquid is obtained by inoculating 0.05 to 0.2 percent of activated cellulomonas flavigena seed liquid to a liquid culture medium and culturing the seed liquid for 16 to 48 hours at the temperature of between 26 and 30 DEG C. Compared with the house fly larva feed without containing the cellulomonas flavigena, the house fly larva feed containing cellulomonas flavigena has the advantages that: the biological yield obtained by the same weight of house fly larva feed is 1.23 to 2.21 times that of control feed without containing the cellulomonas flavigena, the conversion rate of wheat bran is 1.23 to 2.22 times that of a control without containing the cellulomonas flavigena, the utilization rate of the feed is remarkably improved, and the purposes of increasing yield and income are fulfilled. The house fly larva feed has no adverse effects on survival and pupation of maggots, eclosion of pupae, oviposition of feather adults and the like in a recommended range. The house fly larva feed can be used in artificial culture and industrialized production.
Description
Technical field
The present invention relates to a kind of housefly larvae feeds, relate to specifically a kind of housefly larvae feeds that produces the yellowish fiber monad that contains.
Background technology
Housefly is a kind of people's of disturbing sanitary insect pest, also be a kind of resource insect of propagating artificially simultaneously, can be the mankind provides " antibacterial peptide " and some natural polymers of good animal protein, high-strength bactericidal action, such as chitin, shitosan etc., pay attention to so the cultivation of housefly is subject to people day by day, it is propagated artificially and even suitability for industrialized production also has report.But in the propagating artificially of common house-fly grub (claiming again fly maggot), exist the not high problem of conversion ratio of feed, have research to find to raise in the remaining feed of fly maggot and still have a lot of unemployed nutriments.Improving cultivation efficient and efficiency of feed utilization, acquisition high yield is housefly industrialization cultivation urgent problem.As a kind of animal protein, how to improve its quality simultaneously, reach the common aspiration that the purpose that increases income is the raiser.
Producing yellowish fiber monad (Cellulomonas flavigena) is the type sepecies of Cellulomonas.Cellulomonas cell in children's age culture is elongated irregular bacillus, 0.5～0.6 μ m * 2.0～5.0 μ m, until slightly curved, the V word shape that is that has is arranged, accidental branch but without filamentous.The bar of old culture shortens usually, has the minority globuli cell to occur; Gram-positive, but fugitive color; Chang Yiyi root or minority flagellum movement; Do not give birth to spore, not antiacid; Amphimicrobian, the bacterial strain that has under anaerobic can be grown but be very poor; Bacterium colony on peptone-yeast extract agar is projection usually, and is faint yellow; Be chemoheterotrophic bacteria, but but also fermentating metabolism of respiratory metabolism; Under aerobic and anaerobic conditions, all produce acid from glucose and other carbohydrate; Catalase is positive; Reducible nitrate is to nitrite; 28-30 ℃ of its optimum growth temperature.
The achievement that improves the fly-maggot feed utilization rate by the method that contains certain volume production yellowish fiber monad in fly-maggot feed has no report.
Summary of the invention
The purpose of this invention is to provide a kind of efficiently, safe, contain the housefly larvae feeds that produces the yellowish fiber monad.
The present invention is by adding a certain amount of product yellowish fiber monad liquid in housefly larvae feeds commonly used, obtain a kind of new housefly larvae feeds, through behind this forage feed, can effectively improve output that fly maggot raises and the utilization ratio of feed, thereby realize purpose of the present invention.
A kind of housefly larvae feeds that produces the yellowish fiber monad that contains of the present invention, by gross mass mark 100%, the product yellowish fiber monad liquid by 0.6%～65% and 35%～99.4% housefly larvae feeds component commonly used form; Described product yellowish fiber monad liquid be will activation product yellowish fiber monad seed liquor be inoculated in the fluid nutrient medium as 0.05%～0.2% take inoculum concentration, cultivation temperature is 26～30 ℃, incubation time is to obtain in 16～48 hours.
The housefly larvae feeds that produces the yellowish fiber monad that contains of the present invention, by gross mass mark 100%, be 3-65% by producing yellowish fiber monad liquid measure preferably, the amount of housefly larvae feeds component commonly used is 35%～97% composition, the cultivation temperature of producing in the yellowish fiber monad seed liquor access fluid nutrient medium is 26～28 ℃, and incubation time is 16～24 hours.Further preferred product yellowish fiber monad liquid measure is 13%, and the amount of housefly larvae feeds component commonly used is 87%, and inoculum concentration is 0.1%, and cultivation temperature is 26～28 ℃, and incubation time is 16 hours.
The housefly larvae feeds component commonly used that contains in the housefly larvae feeds composition that produces the yellowish fiber monad of the present invention is housefly larvae feeds commonly used, preferably formed by wheat bran and water, because producing yellowish fiber monad liquid is liquid, contain moisture, in order to reach the requirement of the conventional moisture content of wheat bran, of the present invention contain produce the yellowish fiber monad housefly larvae feeds preferably by wheat bran and liquid composition according to mass ratio 35～40: 60～65 form, and described liquid composition is water and product yellowish fiber monad liquid.
The preparation method who contains the housefly larvae feeds that produces the yellowish fiber monad of the present invention activates first to produce the yellowish fiber monad, then will activate the seed liquor of producing the yellowish fiber monad, be inoculated in the fluid nutrient medium, obtain to produce yellowish fiber monad liquid, then after will producing yellowish fiber monad liquid and water mixing, mix with wheat bran again, stir, contain the housefly larvae feeds that produces the yellowish fiber monad and form.
Utilize the housefly larvae feeds that produces the yellowish fiber monad common house-fly grub of feeding that contains of the present invention, compare with the contrast that does not contain the housefly larvae feeds that produces the yellowish fiber monad, the biological yield that the housefly larvae feeds of identical weight obtains, what contain that the housefly larvae feeds that produces the yellowish fiber monad feeds is not contain 1.23～2.21 times that the contrast of producing the yellowish fiber monad is fed, the conversion ratio of wheat bran is not contain 1.23～2.22 times of the contrast of producing yellowish fiber monad liquid, significantly improve the utilization rate of feed, reached the purpose of increasing both production and income.And in recommended range contained product yellowish fiber monad amount to the survival of fly maggot, pupate, laying eggs etc. of the emergence of pupa and emergence adult thereof all have no adverse effects.Of the present inventionly contain the utilization rate that the housefly larvae feeds that produces the yellowish fiber monad can improve feed significantly, reached the purpose of increasing both production and income, therefore can be used for propagating artificially so that suitability for industrialized production in.
The specific embodiment
Following examples are to further specify of the present invention, are not limitations of the present invention.
Embodiment one:
According to conventional method, to produce the activation of yellowish fiber monad, obtain seed liquor, seed liquor is inoculated in the LB culture medium, inoculum concentration is 0.2%, cultivation temperature 26-28 ℃, incubation time is 24 hours, acquisition contains the bacterium liquid that produces the yellowish fiber monad, measures by 0.65% of the gross mass that contains the housefly larvae feeds that produces the yellowish fiber monad and produces yellowish fiber monad liquid, and it is mixed with water first, add again in the wheat bran, stir, configuration forms and contains the housefly larvae feeds 1kg that produces the yellowish fiber monad thus, and wheat bran and water account for 99.35% of gross mass in this feed, wheat bran and liquid (water+product yellowish fiber monad liquid) mix according to mass ratio at 35: 65, above-mentioned feed is put into fly maggot raise box, repeat 3 times, as experimental group.Preparation does not contain fly-maggot feed commonly used (wheat bran and water mix according to mass ratio at 35: the 65) 1kg that produces yellowish fiber monad liquid in addition, puts into fly maggot and raises box, repeats 3 times, in contrast group.With the feed for preparing after placing 5 hours under the condition of indoor (natural temperature: 23-29 ℃), in experimental group and each fly maggot raising box of control group, put into fly blow 0.9g respectively, and with the feed of raising in the box ovum is covered about 1cm, at indoor ((natural temperature: 23-29 ℃), illumination in 12 hours) raised 3.5 days under the condition, feed and larva in every raising box are separated, the weight of larva is all isolated in weighing respectively, get at random wherein certain number larva, weigh its weight, and get at random respectively wherein 10 of larvas, killed in 3 minutes with 80 ℃ of hot water, it is long to measure its body.The weight (fresh weight) that obtains according to the fresh material conversion ratio of the feed=raising/computational methods of feed weight * 100% are calculated respectively the conversion ratio of the long situation of body, fresh feed of weight (fresh weight), the larva of each output (fresh weight) of processing the results larva, 50 cephalonts and wheat bran conversion ratio etc.The results are shown in Table 1.
Table 1. contains 0.65% and produces the housefly larvae feeds of yellowish fiber monad liquid and the production target situation that fly-maggot feed commonly used is raised fly maggot
Containing 0.65% output of producing the housefly larvae feeds of yellowish fiber monad liquid is 1.03 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, and the wheat bran conversion ratio is 1.03 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output.
Embodiment two:
According to conventional method, to produce the activation of yellowish fiber monad, obtain seed liquor, seed liquor is inoculated in the LB culture medium, inoculum concentration is 0.1%, cultivation temperature 26-28 ℃, incubation time is 24 hours, acquisition contains the bacterium liquid that produces the yellowish fiber monad, measures by 3% of the gross mass that contains the housefly larvae feeds that produces the yellowish fiber monad and produces yellowish fiber monad liquid, and it is mixed with water first, add again in the wheat bran, stir, configuration forms and contains the housefly larvae feeds 1kg that produces the yellowish fiber monad thus, and wheat bran and water account for 97% in this feed, wheat bran and liquid (water+product yellowish fiber monad liquid) mix according to mass ratio at 35: 65, above-mentioned feed is put into fly maggot raise box, repeat 3 times, as experimental group.Preparation does not contain fly-maggot feed commonly used (wheat bran and water mix according to mass ratio at 35: the 65) 1kg that produces yellowish fiber monad liquid in addition, puts into fly maggot and raises box, repeats 3 times, in contrast group.With the feed for preparing after placing 5 hours under the condition of indoor (natural temperature: 23-29 ℃), in experimental group and each fly maggot raising box of control group, put into fly blow 0.9g respectively, and with the feed of raising in the box ovum is covered about 1cm, at indoor ((natural temperature: 23-29 ℃), illumination in 12 hours) raised 3.5 days under the condition, feed and larva in every raising box are separated, the weight of larva is all isolated in weighing respectively, get at random wherein certain number larva, weigh its weight, and get at random respectively wherein 10 of larvas, killed in 3 minutes with 80 ℃ of hot water, it is long to measure its body.The weight (fresh weight) that obtains according to the fresh material conversion ratio of the feed=raising/computational methods of feed weight * 100% are calculated respectively the conversion ratio of the long situation of body, fresh feed of weight (fresh weight), the larva of each output (fresh weight) of processing the results larva, 50 cephalonts and wheat bran conversion ratio etc.The results are shown in Table 2.
Table 2. contains 3% and produces the housefly larvae feeds of yellowish fiber monad liquid and the production target situation that fly-maggot feed commonly used is raised fly maggot
Containing the 3% fly maggot output of producing the housefly larvae feeds of yellowish fiber monad liquid is 1.52 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, the wheat bran conversion ratio is 1.52 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, has improved 18 percentage points.
Embodiment three:
According to conventional method, to produce the activation of yellowish fiber monad, obtain seed liquor, seed liquor is inoculated in the LB culture medium, inoculum concentration is 0.1%, cultivation temperature 26-28 ℃, incubation time is 16 hours, acquisition contains the bacterium liquid that produces the yellowish fiber monad, measures by 13% of the gross mass that contains the housefly larvae feeds that produces the yellowish fiber monad and produces yellowish fiber monad liquid, and it is mixed with water first, add again in the wheat bran, stir, configuration forms and contains the housefly larvae feeds 4kg that produces the yellowish fiber monad thus, and wheat bran and water account for 87% of quality in this feed, wheat bran and liquid (water+product yellowish fiber monad liquid) mix according to mass ratio at 35: 65, put into fly maggot and raise box, repeat 3 times, as experimental group.Preparation does not contain fly-maggot feed commonly used (wheat bran and water mix according to mass ratio at 35: the 65) 4kg that produces yellowish fiber monad liquid in addition, puts into fly maggot and raises box, repeats 3 times, in contrast group.With the feed for preparing indoor (after placing 5 hours under the condition of air-conditioning control temperature (25～27 ℃), in experimental group and each fly maggot raising box of control group, put into fly blow 3.5g respectively, and with the feed of raising in the box ovum is covered about 1cm, in indoor (air-conditioning control temperature (25～27 ℃), illumination in 12 hours) raised 3.5 days under the condition, feed and larva in every raising box are separated, the weight of larva is all isolated in weighing respectively, get at random wherein certain number larva, weigh its weight, and get at random respectively wherein 10 of larvas, killed in 3 minutes with 80 ℃ of hot water, it is long to measure its body.The weight (fresh weight) that obtains according to the fresh material conversion ratio of the feed=raising/computational methods of feed weight * 100% are calculated respectively the long situation of body, and the conversion ratio of feed etc. of weight (fresh weight), the larva of each output (fresh weight) of processing the results larva, 50 cephalonts, and utilize SPSS software to analyze.The results are shown in Table 3.
Table 3. contains 13% and produces the housefly larvae feeds of yellowish fiber monad liquid and the production target situation that fly-maggot feed commonly used is raised fly maggot
Containing the 13% fly maggot output of producing the housefly larvae feeds of yellowish fiber monad liquid is 2.21 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, the wheat bran conversion ratio is 2.17 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, has increased by 37.67 percentage points wheat bran conversion ratio.
Embodiment four:
According to conventional method, to produce the activation of yellowish fiber monad, obtain seed liquor, seed liquor is inoculated in the LB culture medium, inoculum concentration is 0.1%, cultivation temperature 26-28 ℃, incubation time is 16 hours, acquisition contains the bacterium liquid that produces the yellowish fiber monad, measures by 39% of the gross mass that contains the housefly larvae feeds that produces the yellowish fiber monad and produces yellowish fiber monad liquid, and it is mixed with water first, add again in the wheat bran, stir, configuration forms and contains the housefly larvae feeds 4kg that produces the yellowish fiber monad thus, and wheat bran and water account for 61% of gross mass in this feed, wheat bran and liquid (water+product yellowish fiber monad liquid) mix according to mass ratio at 35: 65, above-mentioned feed is put into fly maggot raise box, repeat 3 times, as experimental group.Preparation does not contain fly-maggot feed commonly used (wheat bran and water mix according to mass ratio at 35: the 65) 4kg that produces yellowish fiber monad liquid in addition, puts into fly maggot and raises box, repeats 3 times, in contrast group.With the feed for preparing indoor (after placing 5 hours under the condition of air-conditioning control temperature (25～27 ℃), in experimental group and each fly maggot raising box of control group, put into fly blow 3.5g respectively, and with the feed of raising in the box ovum is covered about 1cm, in indoor (air-conditioning control temperature (25～27 ℃), illumination in 12 hours) raised 3.5 days under the condition, feed and larva in every raising box are separated, the weight of larva is all isolated in weighing respectively, get at random wherein certain number larva, weigh its weight, and get at random respectively wherein 10 of larvas, killed in 3 minutes with 80 ℃ of hot water, it is long to measure its body.The weight (fresh weight) that obtains according to the fresh material conversion ratio of the feed=raising/computational methods of feed weight * 100% are calculated respectively the long situation of body, and the conversion ratio of feed etc. of weight (fresh weight), the larva of each output (fresh weight) of processing the results larva, 50 cephalonts, and utilize SPSS software to analyze.The results are shown in Table 4.
Table 4. contains 39% and produces the housefly larvae feeds of yellowish fiber monad liquid and the production target situation that fly-maggot feed commonly used is raised fly maggot
Containing the 39% fly maggot output of producing the housefly larvae feeds of yellowish fiber monad liquid is 2.07 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, the wheat bran conversion ratio is 2.03 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, has increased by 33.34 percentage points wheat bran conversion ratio.
Embodiment five:
According to conventional method, to produce the activation of yellowish fiber monad, obtain seed liquor, seed liquor is inoculated in the LB culture medium, inoculum concentration is 0.1%, cultivation temperature 26-28 ℃, incubation time is 16 hours, obtains to contain the bacterium liquid that produces the yellowish fiber monad, measure product yellowish fiber monad liquid by 65% of the gross mass that contains the housefly larvae feeds that produces the yellowish fiber monad, it is added in the wheat bran, stir, configuration forms and contains the housefly larvae feeds 4kg that produces the yellowish fiber monad thus, wheat bran accounts for 35% in this feed, above-mentioned feed is put into fly maggot raise box, repeat 3 times, as experimental group.Preparation does not contain fly-maggot feed commonly used (wheat bran and water mix according to mass ratio at 35: the 65) 4kg that produces the yellowish fiber monad in addition, puts into fly maggot and raises box, repeats 3 times, in contrast group.With the feed for preparing indoor (after placing 5 hours under the condition of air-conditioning control temperature (25～27 ℃), in experimental group and each fly maggot raising box of control group, put into fly blow 3.5g respectively, and with the feed of raising in the box ovum is covered about 1cm, in indoor (air-conditioning control temperature (25～27 ℃), illumination in 12 hours) raised 3.5 days under the condition, feed and larva in every raising box are separated, the weight of larva is all isolated in weighing respectively, get at random wherein certain number larva, weigh its weight, and get at random respectively wherein 10 of larvas, killed in 3 minutes with 80 ℃ of hot water, it is long to measure its body.The weight (fresh weight) that obtains according to the fresh material conversion ratio of the feed=raising/computational methods of feed weight * 100% are calculated respectively the long situation of body, and the conversion ratio of feed etc. of weight (fresh weight), the larva of each output (fresh weight) of processing the results larva, 50 cephalonts, and utilize SPSS software to analyze.The results are shown in Table 5.
Table 5. contains 65% and produces the housefly larvae feeds of yellowish fiber monad liquid and the production target situation that fly-maggot feed commonly used is raised fly maggot
Containing the 65% fly maggot output of producing the housefly larvae feeds of yellowish fiber monad liquid is 1.99 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, the wheat bran conversion ratio is 1.95 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, has increased by 30.67 percentage points wheat bran conversion ratio.
Embodiment six:
According to conventional method, will produce the activation of yellowish fiber monad, obtain seed liquor, seed liquor is inoculated in the LB culture medium, inoculum concentration is 0.1%, cultivation temperature 26-28 ℃, incubation time is 32 hours, obtains to contain the bacterium liquid that produces the yellowish fiber monad.Measure product yellowish fiber monad liquid by 26% of the gross mass that contains the housefly larvae feeds that produces the yellowish fiber monad, it is mixed with water first, add again in the wheat bran, stir, configuration forms and contains the housefly larvae feeds 2kg that produces the yellowish fiber monad thus, and wheat bran and water account for 74% of gross mass in this feed, wheat bran and liquid (water+product yellowish fiber monad liquid) mix according to mass ratio at 35: 65, above-mentioned feed is put into fly maggot raise box, repeat 3 times, as experimental group.Preparation does not contain fly-maggot feed commonly used (wheat bran and water mix according to mass ratio at 35: the 65) 2kg that produces the yellowish fiber monad in addition, puts into fly maggot and raises box, repeats 3 times, in contrast group.With the feed for preparing after placing 5 hours under the condition of indoor (natural temperature: 19-25 ℃), in experimental group and each fly maggot raising box of control group, put into fly blow 1.8g respectively, and with the feed of raising in the box ovum is covered about 1cm, at indoor ((natural temperature: 19-25 ℃), illumination in 12 hours) raised 3.5 days under the condition, feed and larva in every raising box are separated, the weight of larva is all isolated in weighing respectively, get at random wherein certain number larva, weigh its weight, and get at random respectively wherein 10 of larvas, killed in 3 minutes with 80 ℃ of hot water, it is long to measure its body.The weight (fresh weight) that obtains according to the fresh material conversion ratio of the feed=raising/computational methods of feed weight * 100% are calculated respectively the long situation of body, and the conversion ratio of feed etc. of weight (fresh weight), the larva of each output (fresh weight) of processing the results larva, 50 cephalonts, and utilize SPSS software to analyze.The results are shown in Table 6.
Table 6. contains 26% and produces the housefly larvae feeds of yellowish fiber monad liquid and the production target situation that fly-maggot feed commonly used is raised fly maggot
Containing the 26% fly maggot output of producing the housefly larvae feeds of yellowish fiber monad liquid is 1.93 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, the wheat bran conversion ratio is 1.95 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, has increased by 23 percentage points wheat bran conversion ratio.
Embodiment seven:
According to conventional method, to produce the activation of yellowish fiber monad, obtain seed liquor, seed liquor is inoculated in the LB culture medium, inoculum concentration is 0.05%, cultivation temperature 26-28 ℃, incubation time is 48 hours, acquisition contains the bacterium liquid that produces the yellowish fiber monad, measures by 26% of the gross mass that contains the housefly larvae feeds that produces the yellowish fiber monad and produces yellowish fiber monad liquid, and it is mixed with water first, add again in the wheat bran, stir, configuration forms and contains the housefly larvae feeds 2kg that produces the yellowish fiber monad thus, and wheat bran and water account for 74% of gross mass in this feed, wheat bran and liquid (water+product yellowish fiber monad liquid) mix according to mass ratio at 35: 65, above-mentioned feed is put into fly maggot raise box, repeat 3 times, as experimental group.Preparation does not contain fly-maggot feed commonly used (wheat bran and water mix according to mass ratio at 35: the 65) 2kg that produces the yellowish fiber monad in addition, puts into fly maggot and raises box, repeats 3 times, in contrast group.With the feed for preparing after placing 5 hours under the condition of indoor (natural temperature: 19-25 ℃), in experimental group and each fly maggot raising box of control group, put into fly blow 1.8g respectively, and with the feed of raising in the box ovum is covered about 1cm, at indoor ((natural temperature: 19-25 ℃), illumination in 12 hours) raised 3.5 days under the condition, feed and larva in every raising box are separated, the weight of larva is all isolated in weighing respectively, get at random wherein certain number larva, weigh its weight, and get at random respectively wherein 10 of larvas, killed in 3 minutes with 80 ℃ of hot water, it is long to measure its body.The weight (fresh weight) that obtains according to the fresh material conversion ratio of the feed=raising/computational methods of feed weight * 100% are calculated respectively the long situation of body, and the conversion ratio of feed etc. of weight (fresh weight), the larva of each output (fresh weight) of processing the results larva, 50 cephalonts, and utilize SPSS software to analyze.The results are shown in Table 7.
Table 7. contains 26% and produces the housefly larvae feeds of yellowish fiber monad liquid and the production target situation that fly-maggot feed commonly used is raised fly maggot
Containing the 26% fly maggot output of producing the housefly larvae feeds of yellowish fiber monad liquid is 1.37 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, the wheat bran conversion ratio is 1.38 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, has increased by 9.34 percentage points wheat bran conversion ratio.
Embodiment eight:
According to conventional method, to produce the activation of yellowish fiber monad, obtain seed liquor, seed liquor is inoculated in the LB culture medium, inoculum concentration is 0.1%, cultivation temperature 28-30 ℃, incubation time is 36 hours, acquisition contains the bacterium liquid that produces the yellowish fiber monad, measures by 0.6% of the gross mass that contains the housefly larvae feeds that produces the yellowish fiber monad and produces yellowish fiber monad liquid, and it is mixed with water first, add again in the wheat bran, stir, configuration forms and contains the housefly larvae feeds 1kg that produces the yellowish fiber monad thus, and wheat bran and water account for 99.4% in this feed, wheat bran and liquid (water+product yellowish fiber monad liquid) mix according to mass ratio at 40: 60, put into fly maggot and raise box, repeat 3 times, as experimental group.Preparation does not contain fly-maggot feed commonly used (wheat bran and water mix according to mass ratio at 40: the 60) 1kg that produces the yellowish fiber monad in addition, puts into fly maggot and raises box, repeats 3 times, in contrast group.With the feed for preparing after placing 5 hours under the condition of indoor (temperature: 25-29 ℃), in experimental group and each fly maggot raising box of control group, put into fly blow 0.9g respectively, and with the feed of raising in the box ovum is covered about 1cm, at indoor ((temperature: 25-29 ℃), illumination in 12 hours) raised 3.5 days under the condition, feed and larva in every raising box are separated, the weight of larva is all isolated in weighing respectively, get at random wherein certain number larva, weigh its weight, and get at random respectively wherein 10 of larvas, killed in 3 minutes with 80 ℃ of hot water, it is long to measure its body.The weight (fresh weight) that obtains according to the fresh material conversion ratio of the feed=raising/computational methods of feed weight * 100% are calculated respectively the conversion ratio of the long situation of body, fresh feed of weight (fresh weight), the larva of each output (fresh weight) of processing the results larva, 50 cephalonts and wheat bran conversion ratio etc.The results are shown in Table 8.
Table 8. contains 0.6% and produces the housefly larvae feeds of yellowish fiber monad liquid and the production target situation that fly-maggot feed commonly used is raised fly maggot
Containing the 0.6% fly maggot output of producing the housefly larvae feeds of yellowish fiber monad liquid is 1.23 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, the wheat bran conversion ratio is 1.23 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, has increased by 8 percentage points wheat bran conversion ratio.
Embodiment nine:
According to conventional method, to produce the activation of yellowish fiber monad, obtain seed liquor, seed liquor is inoculated in the LB culture medium, inoculum concentration is 0.1%, cultivation temperature 28-30 ℃, incubation time is 36 hours, acquisition contains the bacterium liquid that produces the yellowish fiber monad, measures by 12% of the gross mass that contains the housefly larvae feeds that produces the yellowish fiber monad and produces yellowish fiber monad liquid, and it is mixed with water first, add again in the wheat bran, stir, configuration forms and contains the housefly larvae feeds 1kg that produces the yellowish fiber monad thus, and wheat bran and water account for 88% of gross mass in this feed, wheat bran and liquid (water+product yellowish fiber monad liquid) mix according to mass ratio at 40: 60, put into fly maggot and raise box, repeat 3 times, as experimental group.Preparation does not contain fly-maggot feed commonly used (wheat bran and water mix according to mass ratio at 40: the 60) 1kg that produces the yellowish fiber monad in addition, puts into fly maggot and raises box, repeats 3 times, as a kind of control group; Simultaneously preparation does not contain produces yellowish fiber monad liquid, and with fly-maggot feed (wheat bran and LB culture medium mix according to mass ratio at 40: the 60) 1kg of the LB culture medium place of water of sterilization, puts into fly maggot and raise box, repeats 3 times, as another kind of control group.With the feed for preparing after placing 5 hours under the condition of indoor (temperature: 25-29 ℃), in experimental group and each fly maggot raising box of control group, put into fly blow 0.9g respectively, and with the feed of raising in the box ovum is covered about 1cm, at indoor ((temperature: 25-29 ℃), illumination in 12 hours) raised 3.5 days under the condition, feed and larva in every raising box are separated, the weight of larva is all isolated in weighing respectively, get at random wherein certain number larva, weigh its weight, and get at random respectively wherein 10 of larvas, killed in 3 minutes with 80 ℃ of hot water, it is long to measure its body.The weight (fresh weight) that obtains according to the fresh material conversion ratio of the feed=raising/computational methods of feed weight * 100% are calculated respectively the conversion ratio of the long situation of body, fresh feed of weight (fresh weight), the larva of each output (fresh weight) of processing the results larva, 50 cephalonts and wheat bran conversion ratio etc.The results are shown in Table 9.
Table 9. contains housefly larvae feeds and the fly-maggot feed commonly used of 12% product yellowish fiber monad liquid and raises the production target situation of fly maggot with the fly-maggot feed of sterilization LB culture medium place of water
Containing the 12% fly maggot output of producing the housefly larvae feeds of yellowish fiber monad liquid is 1.53 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, is not contain to produce yellowish fiber monad liquid but with 1.23 times of the fly-maggot feed raising fly maggot output of sterilization LB culture medium place of water configuration; The wheat bran conversion ratio is 1.53 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, increased by 18 percentage points wheat bran conversion ratio, not contain to produce yellowish fiber monad liquid but with 1.23 times of the fly-maggot feed raising fly maggot output of sterilization LB culture medium place of water configuration, increased by 9.7 percentage points wheat bran conversion ratio, and contain 12% use amount of producing LB culture medium in the housefly larvae feeds of yellowish fiber monad liquid be do not contain LB culture medium in the fly-maggot feed that produces yellowish fiber monad liquid but dispose with sterilization LB culture medium place of water use amount 1/5th.
Embodiment ten:
According to conventional method, to produce the activation of yellowish fiber monad, obtain seed liquor, seed liquor is inoculated in the LB culture medium, inoculum concentration is 0.1%, cultivation temperature 28-30 ℃, incubation time is 36 hours, obtains to contain the bacterium liquid that produces the yellowish fiber monad, measures by 60% of the gross mass that contains the housefly larvae feeds that produces the yellowish fiber monad and produces yellowish fiber monad liquid, mix with wheat bran (account for gross mass 40%), stir, configuration forms and contains the housefly larvae feeds 1kg that produces the yellowish fiber monad thus, above-mentioned feed is put into fly maggot raise box, repeat 3 times, as experimental group.Preparation does not contain fly-maggot feed commonly used (wheat bran and water mix according to mass ratio at 40: the 60) 1kg that produces yellowish fiber monad liquid in addition, puts into fly maggot and raises box, repeats 3 times, as a kind of control group; Simultaneously preparation does not contain produces yellowish fiber monad liquid, and with fly-maggot feed (wheat bran and LB culture medium mix according to mass ratio at 40: the 60) 1kg of the LB culture medium place of water of sterilization, puts into fly maggot and raise box, repeats 3 times, as another kind of control group.With the feed for preparing after placing 5 hours under the condition of indoor (temperature: 25-29 ℃), in experimental group and each fly maggot raising box of control group, put into fly blow 0.9g respectively, and with the feed of raising in the box ovum is covered about 1cm, at indoor ((temperature: 25-29 ℃), illumination in 12 hours) raised 3.5 days under the condition, feed and larva in every raising box are separated, the weight of larva is all isolated in weighing respectively, get at random wherein certain number larva, weigh its weight, and get at random respectively wherein 10 of larvas, killed in 3 minutes with 80 ℃ of hot water, it is long to measure its body.The weight (fresh weight) that obtains according to the fresh material conversion ratio of the feed=raising/computational methods of feed weight * 100% are calculated respectively the conversion ratio of the long situation of body, fresh feed of weight (fresh weight), the larva of each output (fresh weight) of processing the results larva, 50 cephalonts and wheat bran conversion ratio etc.The results are shown in Table 10.
Table 10. contains housefly larvae feeds and the fly-maggot feed commonly used of 60% product yellowish fiber monad liquid and raises the production target situation of fly maggot with the fly-maggot feed of sterilization LB culture medium place of water
Containing the 60% fly maggot output of producing the housefly larvae feeds of yellowish fiber monad liquid is 1.59 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, is not contain to produce yellowish fiber monad liquid but with 1.28 times of the fly-maggot feed raising fly maggot output of sterilization LB culture medium place of water configuration; The wheat bran conversion ratio is 1.60 times that fly-maggot feed commonly used (do not contain and produce yellowish fiber monad liquid) is raised fly maggot output, increased by 20.7 percentage points wheat bran conversion ratio, not contain to produce yellowish fiber monad liquid but with 1.28 times of the fly-maggot feed raising fly maggot output of sterilization LB culture medium place of water configuration, increased by 12 percentage points wheat bran conversion ratio.
Claims (3)
1. one kind contains the housefly larvae feeds that produces yellowish fiber monad (Cellulomonas flavigena), it is characterized in that, by gross mass mark 100%, the product yellowish fiber monad liquid by 0.6%～65% and 35%～99.4% housefly larvae feeds component commonly used form; Described product yellowish fiber monad liquid be will activation product yellowish fiber monad seed liquor be inoculated in the LB fluid nutrient medium with 0.05%～0.2% inoculum concentration, cultivation temperature is 26～30 ℃, incubation time is to obtain in 16～48 hours.
2. the housefly larvae feeds that produces the yellowish fiber monad that contains according to claim 1, it is characterized in that, the mass fraction of described product yellowish fiber monad liquid is 3%～65%, the mass fraction of described housefly larvae feeds component commonly used is 35%～97%, described cultivation temperature is 26～28 ℃, and described incubation time is 16～24 hours.
3. the housefly larvae feeds that produces the yellowish fiber monad that contains according to claim 2, it is characterized in that, the mass fraction of described product yellowish fiber monad liquid is 13%, the mass fraction of described housefly larvae feeds component commonly used is 87%, described inoculum concentration is 0.1%, and described incubation time is 16 hours.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1213507A (en) * | 1997-10-08 | 1999-04-14 | 李小平 | Straw storage compound inoculating agent and its prodn. method |
| EP1481681A1 (en) * | 2003-05-30 | 2004-12-01 | Claudio De Simone | Lactic acid bacteria combination and compositions thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1213507A (en) * | 1997-10-08 | 1999-04-14 | 李小平 | Straw storage compound inoculating agent and its prodn. method |
| EP1481681A1 (en) * | 2003-05-30 | 2004-12-01 | Claudio De Simone | Lactic acid bacteria combination and compositions thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刁治民，等.微生物单细胞蛋白饲料开发利用概况.《青海草业》.1994,第8卷(第1期),4-7. * |
| 李立.蛋白饲料专利技术概况.《饲料博览》.2004,(第10期),35-37. |
| 蛋白饲料专利技术概况;李立;《饲料博览》;20041031(第10期);35-37 * |
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