CN1213507A

CN1213507A - Straw storage compound inoculating agent and its prodn. method

Info

Publication number: CN1213507A
Application number: CN97119728A
Authority: CN
Inventors: 李小平
Original assignee: Individual
Current assignee: Individual
Priority date: 1997-10-08
Filing date: 1997-10-08
Publication date: 1999-04-14
Anticipated expiration: 2017-10-08
Also published as: CN1089560C

Abstract

An inoculating agent for preparing excellent feed by storage of crops' straw or stalk is a mixture of lactobacillus and its activating agent, compound enzyme and its trigger, rice husk powder, ammonium sulfate and urea, and prepared through culturing lactobacillus strains, adding it to powdered rice husk, drying, and mixing with others. The inoculating agent is mixed with the crops' straw or stalk and the mixture is stored for several days under enclosed condition to obtain the excellent feed.

Description

A kind of straw storage compound inoculating agent and production method thereof

The present invention relates to a kind of straw storage compound inoculating agent and production method thereof, belong to the additive of animal feed.Its raw materials for production comprise lactic acid bacteria, saccharomycete, complex enzyme, actication of culture agent etc., and its production process comprises that the expansion of bacterial classification is cultivated, the preparation of the dry thing of viable bacteria and each component mixing etc.

About the existing patent application of feed addictive, it is made up of complex enzyme, trace element, feeding rare earth addition and saccharomycete as Chinese patent application numbers 93118064.3, it must be operated in the aerobic fermentation mode, and fermentate can not be preserved, and the fermentation heat production causes bigger sugar loss.

The purpose of this invention is to provide a kind of straw storage compound inoculating agent and production method thereof, this production method is through enlarge cultivating and in addition actication of culture agent, plurality of enzymes preparation can be produced complex inoculant through technical process such as viable bacteria drying, component mixing again with bacterial classification.This complex inoculant is added water admix various agricultural crop straws and in addition airtight, can become easy storage, livestock is liked the feed eaten.

The objective of the invention is to realize by following measure: the prescription of Inoculant and the percentage by weight of each component be the dry thing 8-12% of powdered rice hulls viable bacteria that contains lactic acid bacteria, contain the dry thing 5-7% of the powdered rice hulls viable bacteria that produces the yellowish fiber monad, the combination drying viable bacteria thing 4-6% of candida tropicalis and geotrichum candidum, bunch spore stemphylium drying thing 5-7%, complex enzyme (comprising fiber rope enzyme, zytase, pectase, amylase, acid protease) 36-40%,

The mixture 28-32% of ammonium sulfate and urea, the actication of culture agent (comprises glucose, NaCl, KH ₂PO ₄, MgSO ₄.7H ₂O, MnSO ₄.4H ₂O, FeSO ₄.7H ₂O) 2%, anhydrous calcium chloride 1%.

With four kinds of lactic acid bacteria culture solutions of Pediococcus pentosaceus, streptococcus faecalis, Lactobacillus delbrueckii, Lactobacillus plantarum of enlarging cultivation by 1: 2.5: 2.5: 4 weight ratio is mixed with powdered rice hulls (120 order) after sterilization again and is evenly mixed by 1.8: 1 weight ratio, makes viable bacteria drying thing with fluidized bed dryer with the homogenize of 45 ℃ of thermal currents dryings;

Evenly mix platform with enlarging the product yellowish fiber monad nutrient solution of cultivation with the weight ratio that the powdered rice hulls (120 order) after sterilization was pressed 1.8: 1, make the dry thing of viable bacteria with fluidized bed dryer with the dry homogenize of 45 ℃ of thermal currents again.

With the above-mentioned dry thing of viable bacteria that contains lactic acid bacteria and produce the yellowish fiber monad, (comprise glucose, NaCl, KH through mixture, the actication of culture agent of the candida tropicalis of low temperature drying homogenize and geotrichum candidum solid mixture, bunch full stemphylium, multiple holoenzyme (comprising cellulase, zytase, pectase, amylase, acid protease), ammonium sulfate and urea ₂PO ₄, MgSO ₄.7H ₂O, MaSO ₄.4H ₂O, FeSO ₄.7H ₂O), anhydrous calcium chloride mixes packing in proportion and is finished product.

Various enzyme proportions are respectively cellulase 50%, zytase 20%, pectase 16%, amylase 7%, acid protease 7% in the complex enzyme, the weight ratio of ammonium sulfate and urea is 1: 2 in the mixture of ammonium sulfate and urea, and each composition proportion of actication of culture agent is glucose 70%, NaCl ₂1.8%, KH ₂PO ₄5%, MgSO ₄.7H ₂O%, MnSO ₄.4H ₂O1%, FeSO ₄.7H ₂O0.2%.

The mechanism of action of the present invention and advantage are: when straw feed on request (moisture transfers to 65%) add Inoculant and airtight after, aerobic cellulose decomposing bacteria factor amount in the Inoculant is big and utilize oxygen in the bin stock and the nutriment in the Inoculant and abundant growth and breeding, simultaneously the saccharomycete in the Inoculant begins propagation, and very fast both promptly become dominant bacteria in the bin stock.Suppress the propagation of harmful aerobic bacteria such as original Escherichia coli and aerobacteria in the raw material by competition more effectively on the one hand, reduced the nutritive loss of bin stock.On the other hand, because of enzyme (particularly cellulase) is a process relatively slowly to the degraded of raw material, the reduced sugar (particularly glucose) that degraded produces can not satisfy the needs of aerobic microbiological and yeast in this section of falling, the cellulose-decomposing bacteria that is in starvation can quicken the plain enzyme of eccrine fiber, cellulose polysaccharide in the raw material goes to degrade, and the reduced sugar of degraded, most of again by yeast, other aerobic microbiological in the raw material and can utilizing the sheet coccus and the galactococcus of neutral pH ambient growth, impel cellulose-decomposing bacteria to secrete more cellulase more, oxygen in bin stock is depleted, and the activity of all aerobic bacterias stops gradually.The required nitrogenous source of this section of falling aerobic microbiological and Yeast proliferation is provided by inorganic nitrogen contained in the Inoculant (urea and ammonium sulfate), carbon source mainly is that corresponding enzymolysis product (mainly being glucose and wood sugar) provides, thereby mycoprotein and a spot of lactic acid (mainly being the metabolite of sheet coccus and galactococcus) of some have been accumulated, the pH value of bin stock can be reduced to below 5.5, and this process is approximately 30 hours.After this mainly be that amphimicrobian lactic acid bacteria begins the raised growth breeding, utilize the catabolite of cellulase and hemicellulase to make carbon source, utilize acid protease that the various nitrogen base acid that part protein degradation in the raw material forms is made nitrogenous source and continued growth and generation lactic acid, and at this moment more acidproof lactobacillus raised growth and produce more lactic acid, treat pH reduce to 4.2 or following, when lactic acid runs up to 1.2-1.5%, all microorganisms (comprising lactic acid bacteria) are all dead or stop action because of low pH and anaerobic, thereby enter stationary phase, overall process is greatly about 4-6 days.This moment bin stock in except that the mycoprotein that has accumulated some, the unavailable polysaccharose substance of multiple domestic animal is partly degraded, simultaneously, produce the lactic acid of larger amt again, the nutritive value of bin stock increases substantially, palatability also is improved, and makes bin stock have good anaerobism shelf characteric.

The present invention also has following characteristics:

1, increases the volume of active bacteria formulation, guaranteed the mixture homogeneity of final products.

2, utilize the loose structure of powdered rice hulls molecule, in the presence of surfactant, thalline enters in the hole of powdered rice hulls small particle with nutrient solution, makes the thalline that enters hole avoid processing the physical damnification that causes with transport point.

3,, make liquid culture to carry out rapid draing, and needn't use expensive spray drying device with the fluid bed of cheapness because of having used this carrier of powdered rice hulls.

The prescription of the present invention and the technological process of production are seen accompanying drawing.

Embodiment:

Four kinds of lactic acid bacterias of Pediococcus pentosaceus, streptococcus faecalis, Lactobacillus delbrueckii, Lactobacillus plantarum that will enlarge cultivation earlier were by 1: 2.5: 2.5: 4 weight ratio is mixed with powdered rice hulls (120 order) after sterilization again and is evenly mixed by 1.8: 1 weight ratio, be dried to water content with fluidized bed dryer with 45 ℃ of thermal currents and got final product in 8 minutes with the double worm mixer homogenize again, need make 100 kilograms altogether less than 8%.

The product yellowish fiber monad that will enlarge cultivation more evenly mixes by 1.8: 1 part by weight with powdered rice hulls (120 order) after sterilization, use again fluidized bed dryer with 45 ℃ of hot gas to water content less than 8%, got final product in 8 minutes with the double worm mixer homogenize again, make 60 kilograms altogether.

Each component is formulated as follows:

100 kilograms of dry things of powdered rice hulls viable bacteria that contain lactic acid bacteria

Contain 60 kilograms of the dry things of the powdered rice hulls viable bacteria that produces the yellowish fiber monad

Totally 400 kilograms of complex enzymes, wherein 200 kilograms of cellulases, 80 kilograms of zytases, 64 kilograms of pectases, 28 kilograms of amylase, 28 kilograms of acid proteases.

Totally 300 kilograms in the mixture of ammonium sulfate and urea, wherein 100 kilograms in ammonium sulfate, 200 kilograms in urea.

Totally 20 kilograms of actication of culture agent, wherein 14 kilograms of glucose, NaCl4.36 kilogram, KH ₂PO ₄1 kilogram, MgSO ₄.7H ₂O0.4 kilogram, MnSO ₄.4H ₂O0.4 kilogram, FeSO ₄.7H ₂The O0.04 kilogram.

Totally 50 kilograms of candida tropicalis and geotrichum candidum solid hybrid dry matters.

60 kilograms of the dry things of bunch spore stemphylium.

10 kilograms of anhydrous calcium chlorides.

With above-mentioned each component evenly mix, packing is finished product.

Claims

1, a kind of straw storage compound inoculating agent, its prescription comprises lactic acid bacteria, saccharomycete, complex enzyme, actication of culture agent etc., it is characterized in that the prescription of Inoculant and the percentage by weight of each component are the dry thing 8-12% of powdered rice hulls viable bacteria that contains lactic acid bacteria, contain the dry thing 5-7% of the powdered rice hulls viable bacteria that produces the yellowish fiber monad, the hybrid dry matter 4-6% of candida tropicalis and geotrichum candidum, the dry thing 5-7% of bunch spore stemphylium, complex enzyme (comprises cellulase, zytase, pectase, amylase, acid protease) 36-40%, the mixture 28-32% of ammonium sulfate and urea, the actication of culture agent (comprises glucose, NaCl, KH ₂PO ₄, MgSO ₄.7H ₂O, MnSO ₄.4H ₂O, FeSO ₄.7H ₂O) 2%, anhydrous calcium chloride 1%.

2, a kind of production method of straw storage compound inoculating agent, the expansion that comprises bacterial classification is cultivated, preparation and each component of the dry thing of viable bacteria are mixed, it is characterized in that to enlarge the Pediococcus pentosaceus of cultivation, streptococcus faecalis, Lactobacillus delbrueckii, four kinds of lactic acid bacteria culture solutions of Lactobacillus plantarum were in 1: 2.5: 2.5: 4 weight ratio is mixed with powdered rice hulls (120 order) after sterilization again and is evenly mixed in 1.8: 1 ratio, make the dry thing of viable bacteria with fluidized bed dryer with the dry homogenize of 45 ℃ of thermal currents, the product yellowish fiber monad nutrient solution that enlarges cultivation is evenly mixed by 1.8: 1 part by weight with powdered rice hulls (120 order) after sterilization, make the dry thing of viable bacteria with fluidized bed dryer with the dry homogenize of 45 ℃ of thermal currents again, contain lactic acid bacteria and produce candida tropicalis and the geotrichum candidum solid mixture of the dry thing of viable bacteria of yellowish fiber monad through the low temperature drying homogenize with above-mentioned, bunch full stemphylium, complex enzyme (comprises cellulase, zytase, pectase, amylase, acid protease), the mixture of ammonium sulfate and urea, the actication of culture agent (comprises glucose, NaCl, KH ₂PO ₄, MgSO ₄.7H ₂O, MnSO ₄.4H ₂O, FeSO ₄.7H ₂O), anhydrous calcium chloride mixes packing in proportion and is finished product.

3, Inoculant according to claim 1, it is characterized in that various enzyme proportions are respectively cellulase 50% in the complex enzyme, zytase 20%, pectase 16%, amylase 7%, acid protease 7%, the weight ratio of ammonium sulfate and urea is 1: 2 in the mixture of ammonium sulfate and urea, and each composition proportion of actication of culture agent is glucose 70%, NaCl ₂1.8%, KH ₂PO ₄5%, MgSO ₄.7H ₂O%, MnSO ₄.4H ₂O1%, FeSO ₄.7H ₂O0.2%.