CN114521613A - Method for improving quality of whole-plant corn straw silage - Google Patents
Method for improving quality of whole-plant corn straw silage Download PDFInfo
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- CN114521613A CN114521613A CN202210224690.3A CN202210224690A CN114521613A CN 114521613 A CN114521613 A CN 114521613A CN 202210224690 A CN202210224690 A CN 202210224690A CN 114521613 A CN114521613 A CN 114521613A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention belongs to the technical field of biological fermentation feed, and discloses a method for improving the quality of whole-plant corn straw silage, which comprises the following steps: preparing a composite microbial inoculum; and preparing the clausena lansium extract and the pomegranate extract; obtaining a whole corn straw, and cutting or crushing the whole corn straw to obtain a whole corn straw section or whole corn straw powder; adding the prepared clausena lansium extract and the pomegranate extract into the obtained whole corn straw sections or whole corn straw powder, and uniformly mixing by using a stirrer; adding the prepared complex microbial inoculum into the obtained mixture, uniformly mixing, and performing fermentation treatment. The invention can effectively improve the nutritional quality of the silage, enrich the nutritional substances of the silage, and improve the protein content of the silage.
Description
Technical Field
The invention belongs to the technical field of biological fermentation feed, and particularly relates to a method for improving the quality of whole-plant corn straw silage.
Background
At present, with the rapid development of ecological animal husbandry in the directions of scale, standardization and modernization, the processed forage grass and feed is very important and becomes one of important factors influencing the continuous development of animal husbandry in recent years. Under the conditions that the China faces insufficient forage and unbalanced seasonal supply and demand, the development and utilization of silage for animal husbandry are needed to meet the current actual situation and the continuous and healthy development of the animal husbandry.
At present, most silage adopts a natural fermentation mode, but a plurality of unstable factors limit the fermentation effect of the ensiling of the green straws, high-quality silage is difficult to produce, and particularly the nutrition quality and aerobic stability of the silage prepared under the condition of summer high-temperature and high-humidity weather are easily seriously affected.
Through the above analysis, the problems and defects of the prior art are as follows: the existing silage is not high in quality and is unstable.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a method for improving the quality of whole corn straw silage.
The invention is realized by a method for improving the quality of whole corn straw silage, which comprises the following steps:
step one, preparing a complex microbial inoculum; and preparing the clausena lansium extract and the pomegranate extract;
step two, obtaining whole corn straws, and cutting or crushing the whole corn straws to obtain whole corn straw sections or whole corn straw powder;
adding the prepared clausena lansium extract and the pomegranate extract into the obtained whole corn straw sections or whole corn straw powder, and uniformly mixing by using a stirrer;
and step four, adding the prepared complex microbial inoculum into the obtained mixture, uniformly mixing, and performing fermentation treatment.
Further, the preparation of the complex microbial inoculum comprises the following steps:
(1) obtaining strains of various bacteria agents, respectively culturing the obtained strains of the various bacteria agents, and obtaining bacterial liquids of the various bacteria agents;
(2) uniformly mixing the obtained bacterial solutions of various bacteria agents, and adding a certain amount of starch to obtain a composite bacteria agent seed solution containing starch;
(3) and performing mixed fermentation culture on the obtained composite microbial inoculum seed liquid on a culture medium, and performing centrifugation, emulsification and freeze-drying treatment to obtain the composite microbial inoculum.
Further, the plurality of microbial agents include: lactobacillus, pediococcus pentosaceus, yeast.
Further, in the step (1), the culturing includes: culturing at 35 deg.C for 20 h.
Further, the preparing of the clausena lansium extract comprises the following steps:
firstly, cleaning wampee fruits, adding a proper amount of clear water, and crushing the wampee fruits by using a crusher to obtain wampee fruit paste;
secondly, carrying out continuous reflux extraction on the obtained wampee fruit paste under the assistance of ultrasonic waves by using the mixed extracting solution, and filtering to obtain an extracting solution;
and finally, carrying out vacuum concentration on the extracting solution to obtain the clausena lansium extract.
Further, the mixed extracting solution is a mixed extracting solution of hydrochloric acid and petroleum ether.
Further, the hydrochloric acid concentration was 6%.
Further, the preparing of the pomegranate extract comprises:
1) separating pericarpium Granati or fructus Punicae Granati from fresh fructus Punicae Granati, squeezing juice from pericarpium Granati, and filtering to obtain juice and fruit residue;
2) uniformly mixing the pomegranate fruits and the pomace with a certain amount of ethanol, and then extracting to obtain an extracting solution; removing solvent from the obtained extractive solution, and mixing with the pomegranate juice to obtain pomegranate extract.
Further, the filtering process includes: filtration was performed with 600 mesh filter cloth.
Further, the addition amount of the composite microbial inoculum is 3% -6% of the whole corn straw.
In combination with the technical solutions and the technical problems to be solved, please analyze the advantages and positive effects of the technical solutions to be protected in the present invention from the following aspects:
first, aiming at the technical problems existing in the prior art and the difficulty in solving the problems, the technical problems to be solved by the technical scheme of the present invention are closely combined with results, data and the like in the research and development process, and some creative technical effects are brought after the problems are solved. The specific description is as follows:
according to the invention, the compound microbial inoculum and the pomegranate and wampee extracts are added, so that the contents of escherichia coli and the like in the silage can be effectively reduced, and an obvious antibacterial effect is achieved; is beneficial to improving the stability of the silage.
Secondly, considering the technical scheme as a whole or from the perspective of products, the technical effect and advantages of the technical scheme to be protected by the invention are specifically described as follows:
the invention can effectively improve the nutritional quality of the silage, enrich the nutritional substances of the silage, and improve the protein content of the silage.
Drawings
FIG. 1 is a flow chart of a method for improving the quality of whole corn stover silage provided by an embodiment of the invention.
Fig. 2 is a flowchart of a method for preparing a complex microbial inoculum according to an embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
First, an embodiment is explained. This section is an explanatory embodiment expanding on the claims so as to fully understand how the present invention is embodied by those skilled in the art.
As shown in fig. 1, the method for improving the quality of whole corn straw silage provided by the embodiment of the invention comprises the following steps:
s101, preparing a complex microbial inoculum; and preparing the clausena lansium extract and the pomegranate extract;
s102, obtaining whole corn straws, and cutting or crushing the whole corn straws to obtain whole corn straw sections or whole corn straw powder;
s103, adding the prepared clausena lansium extract and the pomegranate extract into the obtained whole corn straw sections or whole corn straw powder, and uniformly mixing by using a stirrer;
and S104, adding the prepared complex microbial inoculum into the obtained mixture, uniformly mixing, and performing fermentation treatment.
As shown in fig. 2, the preparation of the complex microbial inoculum according to the embodiment of the present invention includes:
s201, obtaining strains of various bacteria agents, and culturing the obtained strains of the various bacteria agents at 35 ℃ for 20 hours respectively to obtain bacterial liquids of the various bacteria agents;
s202, uniformly mixing the obtained bacterial liquids of various bacteria agents, and adding a certain amount of starch to obtain a composite bacteria agent seed liquid containing starch;
and S203, performing mixed fermentation culture on the obtained composite microbial inoculum seed solution on a culture medium, and performing centrifugation, emulsification and freeze-drying treatment to obtain the composite microbial inoculum.
The various microbial agents provided by the embodiment of the invention comprise: lactobacillus, pediococcus pentosaceus, yeast.
In the step (1), the culturing comprises: culturing at 35 deg.C for 20 h.
The preparation method of the clausena lansium extract provided by the embodiment of the invention comprises the following steps:
firstly, cleaning wampee fruits, adding a proper amount of clear water, and crushing the wampee fruits by using a crusher to obtain wampee fruit paste;
secondly, carrying out continuous reflux extraction on the obtained wampee fruit paste under the assistance of ultrasonic waves by using the mixed extracting solution, and filtering to obtain an extracting solution;
and finally, carrying out vacuum concentration on the extracting solution to obtain the clausena lansium extract.
The mixed extracting solution provided by the embodiment of the invention is a mixed extracting solution of hydrochloric acid and petroleum ether.
The embodiment of the invention provides a hydrochloric acid concentration of 6%.
The preparation of the pomegranate extract provided by the embodiment of the invention comprises the following steps:
1) separating pericarpium Granati or fructus Punicae Granati from fresh fructus Punicae Granati, squeezing juice from pericarpium Granati, and filtering to obtain juice and fruit residue;
2) uniformly mixing the pomegranate fruits and the pomace with a certain amount of ethanol, and then extracting to obtain an extracting solution; removing solvent from the obtained extractive solution, and mixing with the pomegranate juice to obtain pomegranate extract.
The filtering treatment provided by the embodiment of the invention comprises the following steps: filtration was performed with 600 mesh filter cloth.
The addition amount of the composite microbial inoculum provided by the embodiment of the invention is 3-6% of that of the whole corn straw.
And II, application embodiment. In order to prove the creativity and the technical value of the technical scheme of the invention, the part is an application example of the technical scheme of the claims to a specific product or related technology.
The method for improving the quality of the whole corn straw silage is applied to the preparation of the silage, and the quality of the silage can be effectively improved.
The above description is only for the purpose of illustrating the embodiments of the present invention, and the scope of the present invention should not be limited thereto, and any modifications, equivalents and improvements made by those skilled in the art within the technical scope of the present invention as disclosed in the present invention should be covered by the scope of the present invention.
Claims (10)
1. A method of improving whole corn stover silage quality, comprising:
step one, preparing a complex microbial inoculum; and preparing the clausena lansium extract and the pomegranate extract;
step two, obtaining whole corn straws, and cutting or crushing the whole corn straws to obtain whole corn straw sections or whole corn straw powder;
adding the prepared clausena lansium extract and the prepared pomegranate extract into the obtained whole corn straw sections or whole corn straw powder, and uniformly mixing by using a stirrer;
and step four, adding the prepared complex microbial inoculum into the obtained mixture, uniformly mixing, and performing fermentation treatment.
2. The method for improving the quality of the whole corn straw silage as claimed in claim 1, wherein the preparing of the composite microbial inoculum comprises:
(1) obtaining strains of various bacteria agents, respectively culturing the obtained strains of the various bacteria agents, and obtaining bacterial liquids of the various bacteria agents;
(2) uniformly mixing the obtained bacterial solutions of various bacteria agents, and adding a certain amount of starch to obtain a composite bacteria agent seed solution containing starch;
(3) and performing mixed fermentation culture on the obtained composite microbial inoculum seed liquid on a culture medium, and performing centrifugation, emulsification and freeze-drying treatment to obtain the composite microbial inoculum.
3. The method of improving whole corn stover silage quality of claim 2, wherein the plurality of bacterial agents comprises: lactobacillus, pediococcus pentosaceus, yeast.
4. The method of improving the quality of whole corn stover silage as claimed in claim 2, wherein in step (1), the culturing comprises: culturing at 35 deg.C for 20 h.
5. The method of claim 1, wherein the preparing the flavedo extract comprises:
firstly, cleaning wampee fruits, adding a proper amount of clear water, and crushing the wampee fruits by using a crusher to obtain wampee fruit paste;
secondly, carrying out continuous reflux extraction on the obtained wampee fruit paste under the assistance of ultrasonic waves by using the mixed extracting solution, and filtering to obtain an extracting solution;
and finally, carrying out vacuum concentration on the extracting solution to obtain the clausena lansium extract.
6. The method of claim 5, wherein the mixed extract is a mixture of hydrochloric acid and petroleum ether.
7. The method of improving the quality of whole corn stover silage of claim 6, wherein the hydrochloric acid concentration is 6%.
8. The method of claim 1, wherein the step of preparing the pomegranate extract comprises:
1) separating pericarpium Granati or fructus Punicae Granati from fresh fructus Punicae Granati, squeezing juice from pericarpium Granati, and filtering to obtain juice and fruit residue;
2) uniformly mixing the pomegranate fruits and the pomace with a certain amount of ethanol, and then extracting to obtain an extracting solution; removing solvent from the obtained extractive solution, and mixing with the pomegranate juice to obtain pomegranate extract.
9. The method of improving whole corn stover silage quality of claim 8, wherein the filtration process comprises: filtration was performed with 600 mesh filter cloth.
10. The method for improving the quality of the whole corn stalk silage fodder as claimed in claim 8, wherein the compound bacteria agent is added in 3% -6% of the whole corn stalk.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210224690.3A CN114521613A (en) | 2022-03-07 | 2022-03-07 | Method for improving quality of whole-plant corn straw silage |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210224690.3A CN114521613A (en) | 2022-03-07 | 2022-03-07 | Method for improving quality of whole-plant corn straw silage |
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| CN114521613A true CN114521613A (en) | 2022-05-24 |
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| CN202210224690.3A Pending CN114521613A (en) | 2022-03-07 | 2022-03-07 | Method for improving quality of whole-plant corn straw silage |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9017286D0 (en) * | 1990-08-07 | 1990-09-19 | Ag Bag Corp | Stabilization of silage |
| CN1213507A (en) * | 1997-10-08 | 1999-04-14 | 李小平 | Straw storage compound inoculating agent and its prodn. method |
| CN103913564A (en) * | 2014-04-11 | 2014-07-09 | 苏秀芳 | Method for testing bacteriostatic activity of spire wampee extractive |
| CN108497175A (en) * | 2018-04-17 | 2018-09-07 | 贵州省草地技术试验推广站 | A kind of Silaging method removing fringe maize straw |
-
2022
- 2022-03-07 CN CN202210224690.3A patent/CN114521613A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9017286D0 (en) * | 1990-08-07 | 1990-09-19 | Ag Bag Corp | Stabilization of silage |
| CN1213507A (en) * | 1997-10-08 | 1999-04-14 | 李小平 | Straw storage compound inoculating agent and its prodn. method |
| CN103913564A (en) * | 2014-04-11 | 2014-07-09 | 苏秀芳 | Method for testing bacteriostatic activity of spire wampee extractive |
| CN108497175A (en) * | 2018-04-17 | 2018-09-07 | 贵州省草地技术试验推广站 | A kind of Silaging method removing fringe maize straw |
Non-Patent Citations (1)
| Title |
|---|
| 胡建军 等: "植物提取物体外抑菌试验的研究", 塔里木农垦大学学报, vol. 16, no. 2, pages 7 - 8 * |
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