CN115024393A - Application of fructus amomi essential oil in improving quality of paper mulberry silage - Google Patents
Application of fructus amomi essential oil in improving quality of paper mulberry silage Download PDFInfo
- Publication number
- CN115024393A CN115024393A CN202210742830.6A CN202210742830A CN115024393A CN 115024393 A CN115024393 A CN 115024393A CN 202210742830 A CN202210742830 A CN 202210742830A CN 115024393 A CN115024393 A CN 115024393A
- Authority
- CN
- China
- Prior art keywords
- paper mulberry
- silage
- essential oil
- fructus amomi
- ensiling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000341 volatile oil Substances 0.000 title claims abstract description 54
- 239000004460 silage Substances 0.000 title claims description 86
- 240000006248 Broussonetia kazinoki Species 0.000 title 1
- 241000933832 Broussonetia Species 0.000 claims abstract description 90
- 241000705930 Broussonetia papyrifera Species 0.000 claims description 49
- 241000196324 Embryophyta Species 0.000 claims description 23
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 22
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 21
- 238000002156 mixing Methods 0.000 claims description 17
- 239000004310 lactic acid Substances 0.000 claims description 11
- 235000014655 lactic acid Nutrition 0.000 claims description 11
- 238000007781 pre-processing Methods 0.000 claims description 9
- 235000019750 Crude protein Nutrition 0.000 claims description 8
- 150000001720 carbohydrates Chemical class 0.000 claims description 8
- 239000000835 fiber Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 5
- 238000011049 filling Methods 0.000 claims description 5
- OBMBUODDCOAJQP-UHFFFAOYSA-N 2-chloro-4-phenylquinoline Chemical compound C=12C=CC=CC2=NC(Cl)=CC=1C1=CC=CC=C1 OBMBUODDCOAJQP-UHFFFAOYSA-N 0.000 claims description 4
- 239000003599 detergent Substances 0.000 claims description 4
- 230000007935 neutral effect Effects 0.000 claims description 4
- 239000004698 Polyethylene Substances 0.000 claims description 2
- -1 polyethylene Polymers 0.000 claims description 2
- 229920000573 polyethylene Polymers 0.000 claims description 2
- 238000000855 fermentation Methods 0.000 abstract description 5
- 230000004151 fermentation Effects 0.000 abstract description 5
- 241000186660 Lactobacillus Species 0.000 abstract description 4
- 240000001929 Lactobacillus brevis Species 0.000 abstract description 4
- 235000013957 Lactobacillus brevis Nutrition 0.000 abstract description 4
- 229940039696 lactobacillus Drugs 0.000 abstract description 4
- 230000001580 bacterial effect Effects 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 240000006024 Lactobacillus plantarum Species 0.000 description 7
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 7
- 239000000654 additive Substances 0.000 description 7
- 229940072205 lactobacillus plantarum Drugs 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 5
- 241000186679 Lactobacillus buchneri Species 0.000 description 5
- 230000000996 additive effect Effects 0.000 description 5
- 244000144972 livestock Species 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 244000141331 Amomum villosum Species 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 235000019260 propionic acid Nutrition 0.000 description 3
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 3
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 240000004658 Medicago sativa Species 0.000 description 2
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000013613 poultry product Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
- A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses an application of fructus amomi essential oil in improving paper mulberry ensiling quality, belonging to the technical field of paper mulberry ensiling. When the paper mulberry ensiling feed is ensiled for 60 days, the relative abundance of Levilactobacillus and Lentilactibacillus in the ensiling feed treated by the AVEO is higher than that of other ensiling feeds, the fructus amomi essential oil influences the fermentation quality of the paper mulberry ensiling by changing the bacterial community structure of the paper mulberry ensiling, and the AVEO mainly increases the abundance of lactobacillus brevis and lactobacillus slowianus of the ensiling, thereby improving the quality of the paper mulberry ensiling.
Description
Technical Field
The invention relates to the technical field of paper mulberry silage, in particular to application of fructus amomi essential oil in improving paper mulberry silage quality.
Background
With the strong support of the state on the animal husbandry, the development trend of the animal husbandry is good in recent years, and one of the problems restricting the further development of the animal husbandry is that the feed supply is insufficient, so that the contradiction of food competition between people and livestock is increasingly prominent. In order to solve the demand of livestock for feed, the development and utilization of unconventional novel forage grass resources are imperative. The paper mulberry (Broussonetia papyrifera) has the advantages of fast growth, rich nutrient contents, strong stress resistance and the like, and can improve the disease resistance and the digestibility of livestock due to the rich nutrient substances such as Crude Protein (CP), crude fat (EE) and the like, thereby being a high-quality feed resource. However, the harvest of broussonetia papyrifera biomass is seasonal, with a high accumulation in rainy seasons and with water contents of broussonetia papyrifera up to 68%. Therefore, ensiling is more suitable for keeping paper mulberry trees than traditional hay making.
Food problems have been a major civil problem. Because antibiotics have adverse effects on human bodies, it is imperative to find some safe and environment-friendly additives to replace the antibiotics, and the characteristics of nature, no pollution, no residue and the like of the plant essential oil also become research hotspots. The plant essential oil as an additive is added into the feed to accelerate the growth and development process of animals and improve the quality of livestock and poultry products, and the effect of the plant essential oil on preventing the feed from generating mycotoxin is proved, so that the green and pollution-free livestock and poultry products can be provided. The results of the Chaves et al study show that the addition of plant essential oil to barley silage can increase the soluble carbohydrate (WSC) content of the silage and can reduce the yeast and mold content of the barley silage. Studies of Liwei and the like show that the addition of plant essential oil in alfalfa ensilage can increase the content of Lactic Acid (LA) and the number of lactic acid bacteria, improve the aerobic stability of the alfalfa ensilage, and reduce the pH value and the ammoniacal Nitrogen (NH) 3 -N) content. Fructus amomi essential oil (Amomum villosum Lour. essential oil, AVEO) mainly contains substances such as alkene, ester and alcohol, and the AVEO proves that the fructus amomi essential oil has wide bacteriostatic action, so the fructus amomi essential oil can be used as an antibacterial medicament and also can be used as an antibacterial agentCan be used as natural antiseptic additive in food.
Fresh broussonetia papyrifera samples have the defects of low attached lactic acid bacteria content, low soluble carbohydrate content, high buffer energy value and the like, so that the conventional silage is not easy to succeed, and the silage additive must be added to improve the silage quality. Researches show that the common additives can not improve the silage quality of the paper mulberry, and a new method for improving the silage quality of the paper mulberry needs to be developed. Therefore, the invention provides the application of the fructus amomi essential oil in improving the quality of paper mulberry silage.
Disclosure of Invention
The invention provides application of fructus amomi essential oil in improving the paper mulberry silage quality, effectively solves the technical problem that the paper mulberry silage quality cannot be effectively improved by the existing additive, and simultaneously provides a method for simply and efficiently improving the paper mulberry silage quality.
The invention provides application of fructus amomi essential oil in improving the quality of paper mulberry silage, which is characterized by comprising the following steps:
s1, collecting a paper mulberry: selecting a broussonetia papyrifera with a good growth state, and taking a part of plants for later use;
s2, preprocessing a paper mulberry: cutting the part of the plant of S1 to 1-2 cm to obtain a fresh paper mulberry sample;
s3, ensiling treatment: and (5) adding fructus amomi essential oil into the fresh paper mulberry sample of S2, uniformly mixing, filling into an ensiling bag, vacuumizing, and ensiling to obtain paper mulberry ensiling.
Preferably, in S2, the fresh broussonetia papyrifera sample has a moisture content of 65% to 75%.
Preferably, in S3, the fructus amomi essential oil has a purity of 99%.
Preferably, in S3, the ratio of the fresh broussonetia papyrifera sample to the amomum villosum essential oil is 1 kg: 1-3 ml.
Preferably, in S3, the ensiling treatment temperature is 22 to 26 ℃.
Preferably, in S3, the ensiling treatment time is 30 to 60 days.
Preferably, in S3, the vacuumizing time is 3-5 min, and the vacuum pressure is 50-60 kPa.
Preferably, the dry matter content of the paper mulberry silage is 25.46-27.5% based on fresh paper mulberry samples.
Preferably, based on dry matter of paper mulberry ensiling, the paper mulberry ensiling has crude protein content of 15.46-15.56%, neutral detergent fiber content of 37.87-41.93%, acid detergent fiber content of 35.01-38.13%, soluble carbohydrate content of 1.14-1.18%, ammoniacal nitrogen content of 2.16-2.3%, lactic acid content of 7.26-7.68% and acetic acid content of 3.09-3.33%.
Preferably, in S3, the silage bag is a polyethylene bag having a gauge of 25cm × 30 cm.
Compared with the prior art, the invention has the beneficial effects that:
(1) the method comprises the steps of selecting a paper mulberry plant with a good growth state, cutting the paper mulberry plant into 1-2 cm, adding fructus amomi essential oil (AVEO), uniformly mixing, filling the paper mulberry plant into an ensiling bag, vacuumizing, carrying out ensiling treatment to obtain the silage paper mulberry, and carrying out ensiling for 60 days, wherein the relative abundance of Lactplantibacillus (Lactobacillus plantarum) in the silage treated by LAB and AVEO is higher than that of CK silage. When the silage is ensiled for 60 days, the relative abundance of Levilactobacillus (Lactobacillus brevis) and Lentillactobacillus (Lactobacillus lentus) in the silage treated by AVEO is higher than that of other silage, the fructus amomi essential oil influences the fermentation quality of paper mulberry silage by changing the bacterial community structure of paper mulberry silage, and the AVEO mainly increases the abundance of the silage Lactobacillus brevis and the Lactobacillus lentus, so that the quality of paper mulberry silage is improved; the paper mulberry silage obtained by the treatment of the amomum villosum essential oil has dry matter content of 25.46-27.5%, crude protein content of 15.46-15.56%, neutral detergent fiber content of 37.87-41.93%, acid detergent fiber content of 35.01-38.13%, soluble carbohydrate content of 1.14-1.18%, ammonia nitrogen content of 2.16-2.3%, lactic acid content of 7.26-7.68% and acetic acid content of 3.09-3.33%.
(2) The fructus amomi essential oil provided by the invention is applied to improving the silage quality of paper mulberry, has the advantages of simple silage method, strong practicability and low cost, and can effectively solve the technical problem that the silage quality of paper mulberry cannot be effectively improved by the existing additive.
Drawings
FIG. 1 is a graph of the relative abundance of microorganisms in paper mulberry silage treated with comparative example 1(CK), comparative example 2(LAB) and example 3 (AVEO).
Detailed Description
In order to make the technical solutions of the present invention better understood and enable those skilled in the art to practice the present invention, the following embodiments are further described, but the present invention is not limited to the following embodiments. The following test methods and detection methods, unless otherwise specified, are conventional methods; the reagents and starting materials are all commercially available, unless otherwise specified.
Example 1
The application of the fructus amomi essential oil in improving the quality of paper mulberry silage is characterized by comprising the following steps:
s1, collecting a paper mulberry: selecting a broussonetia papyrifera with a good growth state, and taking a part of plants for later use;
s2, preprocessing a paper mulberry: cutting the partial plant of S1 to 1cm to obtain a fresh Broussonetia papyrifera sample with the water content of 65%;
s3, ensiling treatment: mixing fresh broussonetia papyrifera sample with 1kg of fructus amomi essential oil: adding fructus Amomi essential oil with purity of 99% into fresh Broussonetia papyrifera sample of S2 at a feed-liquid ratio of 1ml, mixing, placing into silage bags of 25cm × 30cm, vacuumizing (vacuumizing time is 3min, and vacuum pressure is 50kPa), and performing silage treatment at 22 deg.C for 30d to obtain Broussonetia papyrifera silage.
Example 2
The application of the fructus amomi essential oil in improving the quality of paper mulberry silage is characterized by comprising the following steps:
s1, collecting a paper mulberry: selecting a broussonetia papyrifera with a good growth state, and taking a part of plants for later use;
s2, preprocessing a paper mulberry: cutting the partial plant of S1 to 2cm to obtain fresh Broussonetia papyrifera sample with water content of 75%;
s3, ensiling treatment: mixing fresh broussonetia papyrifera sample with 1kg of fructus amomi essential oil: adding fructus Amomi essential oil with purity of 99% into fresh Broussonetia papyrifera sample of S2 at a feed-liquid ratio of 3ml, mixing, placing into silage bags of 25cm × 30cm, vacuumizing (vacuumizing time is 5min, and vacuum pressure is 60kPa), and performing silage treatment at 26 deg.C for 45 days to obtain Broussonetia papyrifera silage.
Example 3
The application of the fructus amomi essential oil in improving the quality of paper mulberry silage is characterized by comprising the following steps:
s1, collecting a paper mulberry: selecting a broussonetia papyrifera with a good growth state, and taking a part of plants for later use;
s2, preprocessing a paper mulberry: cutting the partial plant of S1 to 1.5cm to obtain a fresh Broussonetia papyrifera sample with the water content of 65%;
s3, ensiling treatment: mixing fresh broussonetia papyrifera sample with 1kg of fructus amomi essential oil: adding fructus Amomi essential oil with purity of 99% into the fresh Broussonetia papyrifera sample of S2 at a feed-liquid ratio of 2ml, mixing, placing into silage bags of 25cm × 30cm, vacuumizing (vacuumizing time is 4min, and vacuum pressure is 55kPa), and performing silage treatment at 24 deg.C for 60 days to obtain Broussonetia papyrifera silage.
Example 4
The application of the fructus amomi essential oil in improving the quality of paper mulberry silage is characterized by comprising the following steps:
s1, collecting a paper mulberry: selecting a broussonetia papyrifera with a good growth state, and taking a part of plants for later use;
s2, preprocessing a broussonetia tree: cutting the partial plant of S1 to 1.2cm to obtain fresh Broussonetia papyrifera sample with water content of 68%;
s3, ensiling treatment: mixing fresh broussonetia papyrifera sample with 1kg of fructus amomi essential oil: adding fructus Amomi essential oil with purity of 99% into the fresh Broussonetia papyrifera sample of S2 at a feed-liquid ratio of 1.5ml, mixing, placing into silage bags of 25cm × 30cm, vacuumizing for 3.5min under vacuum pressure of 52kPa) and performing silage treatment at 23 deg.C for 37 days to obtain Broussonetia papyrifera silage.
Example 5
The application of the fructus amomi essential oil in improving the quality of paper mulberry silage is characterized by comprising the following steps:
s1, collecting a paper mulberry: selecting a broussonetia papyrifera with a good growth state, and taking a part of plants for later use;
s2, preprocessing a paper mulberry: cutting the partial plant of S1 to 1.8cm to obtain a fresh Broussonetia papyrifera sample with the water content of 72%;
s3, ensiling treatment: mixing fresh broussonetia papyrifera sample with 1kg of fructus amomi essential oil: adding fructus Amomi essential oil with purity of 99% into the fresh Broussonetia papyrifera sample of S2 at a feed-liquid ratio of 2.5ml, mixing, placing into a silage bag of 25cm × 30cm, vacuumizing (vacuumizing time is 4.5min, vacuum pressure is 58kPa) and performing silage treatment at 25 ℃ for 53d to obtain Broussonetia papyrifera silage.
In order to further illustrate the effects of the present invention, the present invention was also provided with comparative examples, as follows:
comparative example 1
The influence of distilled water on the quality of paper mulberry silage comprises the following steps:
s1, collecting a paper mulberry: selecting a broussonetia papyrifera with a good growth state, and taking a part of plants for later use;
s2, preprocessing a paper mulberry: cutting the partial plant of S1 to 1.5cm to obtain a fresh Broussonetia papyrifera sample with the water content of 65%;
s3, ensiling treatment: mixing a fresh broussonetia papyrifera sample with 1kg of distilled water: adding distilled water into the fresh paper mulberry sample in S2 according to the feed-liquid ratio of 2ml, uniformly mixing, filling into an ensiling bag with the thickness of 25cm multiplied by 30cm, vacuumizing (the vacuumizing time is 4min, and the vacuum pressure is 55kPa), and carrying out ensiling treatment for 60 days at 24 ℃ to obtain the paper mulberry ensiling.
Comparative example 2
The application of the mixture of lactobacillus plantarum and lactobacillus buchneri in improving the quality of paper mulberry silage comprises the following steps:
s1, collecting a paper mulberry: selecting a broussonetia papyrifera with a good growth state, and taking a part of plants for later use;
s2, preprocessing a paper mulberry: cutting the partial plant of S1 to 1.5cm to obtain a fresh Broussonetia papyrifera sample with the water content of 65%;
s3, ensiling treatment: according to the ratio of the mixture of fresh broussonetia papyrifera sample and lactobacillus plantarum and lactobacillus buchneri of 1 kg: 2ml of the mixture of lactobacillus plantarum and lactobacillus buchneri in the fresh broussonetia papyrifera sample according to the feed liquid ratio of S2, uniformly mixing, filling into silage bags of 25cm multiplied by 30cm, vacuumizing (the vacuumizing time is 4min, and the vacuum pressure is 55kPa), and carrying out silage treatment at 24 ℃ for 60 days to obtain broussonetia papyrifera silage.
The mixture of the lactobacillus plantarum and the lactobacillus buchneri takes the mass of a paper mulberry sample as a referenceThe effective bacteria is 2 × 10 7 cfu/g。
According to detection, chemical components, fermentation characteristics and bacterial community performances of broussonetia papyrifera ensilage obtained by treatment of fructus amomi essential oil (AVEO) in examples 1-5 are similar, and only example 3 is taken as an example for description, and the invention also provides comparative example 1 and comparative example 2, wherein the weight of distilled water (CK) equal to that of the fructus amomi essential oil is added into a broussonetia papyrifera sample in comparative example 1, and a mixture (LAB) of lactobacillus plantarum and lactobacillus buchneri is added into a fresh broussonetia papyrifera sample in comparative example 2. The detection method and the detection result are as follows.
Detecting chemical components of paper mulberry silage: firstly, ensiling a fresh paper mulberry sample to obtain paper mulberry ensiling, drying at constant temperature of 65 ℃, crushing, sieving by a 0.5mm sieve, and weighing to obtain the Dry Matter (DM) content; crude Protein (CP), Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), and soluble carbohydrate (WSC) were measured in dry matter on a dry matter content basis, respectively, and the results are shown in table 1.
TABLE 1 chemical composition Table of fresh Broussonetia papyrifera samples and Broussonetia papyrifera silage
| Chemical composition | Fresh sample of paper mulberry | Comparative example 1 | Comparative example 2 | Example 3 |
| DM％FM | 28.87±0.08 | 25.83±0.44b | 24.22±0.16b | 26.48±1.02a |
| CP％DM | 15.67±0.49 | 14.98±0.78 | 15.61±0.22 | 15.51±0.05 |
| NDF％DM | 66.70±0.04 | 31.21±0.54b | 37.50±0.91a | 39.90±2.03a |
| ADF％DM | 39.91±1.72 | 25.26±0.73b | 33.65±1.00a | 36.57±1.56a |
| WSC％DM | 2.07±0.04 | 0.71±0.01b | 1.19±0.04a | 1.16±0.02a |
As can be seen from table 1, the Dry Matter (DM) content of the AVEO treated silage compared to comparative example 1 and comparative example 2 is significantly higher than that of the other treated silage (P < 0.05); NDF and ADF levels of LAB and AVEO treated silage were higher than CK silage (P < 0.05); AVEO treatment significantly increased WSC content compared to CK ensilage (P < 0.05).
(II) detecting the fermentation characteristics of paper mulberry silage: broussonetia papyrifera obtained in comparative example 1, comparative example 2 and example 3Weighing 20g of the silage, mixing with 180ml of sterile water for 3min, filtering with 4 layers of coarse cotton cloth to obtain filtrate, and measuring pH value, organic acid and ammonia Nitrogen (NH) of paper mulberry silage 3 -N) content, the contents of Lactic Acid (LA), Acetic Acid (AA), Propionic Acid (PA) and Butyric Acid (BA) were determined using a UV detector at 210nm and an Agilent 1260HPLC system from Acclaim (TM), the results of which are shown in Table 2.
TABLE 2 fermentation characteristics of paper mulberry silage
| Item | Comparative example 1 | Comparative example 2 | Example 3 |
| pH | 5.25±0.01a | 5.31±0.15a | 4.45±0.06b |
| LA(％DM) | 1.30±0.06c | 2.20±0.03b | 7.47±0.21a |
| AA(％DM) | 2.25±0.25c | 3.83±0.04a | 3.21±0.12b |
| PA(％DM) | - | - | - |
| BA(％DM) | - | - | - |
| NH 3 -N(％DM) | 2.44±0.06a | 2.36±0.07b | 2.23±0.07c |
As can be seen from Table 2: during ensiling, the pH of the AVEO-treated ensilage was lower (P) than that of the CK ensilage<0.05); the AVEO-treated silage has the highest LA content (P) compared to other silage<0.05); AVEO treatment significantly reduced NH compared to CK silage 3 -N content (P)<0.05); AVEO treatment significantly increased AA content (P) compared to CK silage<0.05)。
(III) detecting paper mulberry ensiling microorganisms: the microorganisms contained in the broussonetia papyrifera silage obtained in comparative example 1, comparative example 2 and example 3 were tested, and the results are shown in fig. 1.
As can be seen from fig. 1: at 60 days of ensiling, the relative abundance of Lactplantibacillus (Lactobacillus plantarum) was higher than that of CK ensiling in LAB and AVEO treated ensiling. At 60 days of ensiling, the relative abundance of Levilactobacillus (lactobacillus brevis) and lentilabactobacterium (lactobacillus lentus) in the AVEO-treated silage was higher than that of other ensilages.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (10)
1. The application of the fructus amomi essential oil in improving the quality of paper mulberry silage is characterized by comprising the following steps:
s1, collecting a paper mulberry: selecting a broussonetia papyrifera with a good growth state, and taking a part of plants for later use;
s2, preprocessing a broussonetia tree: cutting the part of the plant of S1 to 1-2 cm to obtain a fresh paper mulberry sample;
s3, ensiling treatment: and (4) adding fructus amomi essential oil into the fresh paper mulberry sample of S2, uniformly mixing, filling into an ensiling bag, vacuumizing and ensiling to obtain paper mulberry ensiling.
2. The application of fructus amomi essential oil in improving the silage quality of a paper mulberry, as claimed in claim 1, wherein in S2, the moisture content of the fresh paper mulberry sample is 65% -75%.
3. The use of fructus amomi essential oil for improving the quality of broussonetia papyrifera silage according to claim 1, wherein in S3, the purity of the fructus amomi essential oil is 99%.
4. The application of fructus amomi essential oil in improving the silage quality of paper mulberry according to claim 1, wherein in S3, the feed-liquid ratio of the fresh paper mulberry sample to the fructus amomi essential oil is 1 kg: 1-3 ml.
5. The use of fructus amomi essential oil for improving the quality of paper mulberry silage as claimed in claim 1, wherein in S3, the temperature of silage treatment is 22-26 ℃.
6. The application of fructus amomi essential oil in improving the silage quality of paper mulberry according to claim 1, wherein in S3, the silage treatment time is 30-60 days.
7. The application of fructus amomi essential oil in improving the quality of paper mulberry ensilage as claimed in claim 1, wherein in S3, the vacuumizing time is 3-5 min, and the vacuum pressure is 50-60 hPa.
8. The use of fructus amomi essential oil for improving the quality of paper mulberry silage, as claimed in claim 1, characterized in that the dry matter content of paper mulberry silage is 25.46% -27.5% based on fresh paper mulberry sample.
9. The application of the fructus amomi essential oil in improving the quality of paper mulberry ensiling is characterized in that the crude protein content of the paper mulberry ensiling is 15.46-15.56%, the neutral detergent fiber content is 37.87-41.93%, the acid detergent fiber content is 35.01-38.13%, the soluble carbohydrate content is 1.14-1.18%, the ammonia nitrogen content is 2.16-2.3%, the lactic acid content is 7.26-7.68%, and the acetic acid content is 3.09-3.33% based on the dry matter of the paper mulberry ensiling.
10. The use of fructus amomi essential oil for improving the silage quality of paper mulberry according to claim 1, wherein in S3, the silage bag is a polyethylene bag with the specification of 25cm x 30 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210742830.6A CN115024393A (en) | 2022-06-28 | 2022-06-28 | Application of fructus amomi essential oil in improving quality of paper mulberry silage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210742830.6A CN115024393A (en) | 2022-06-28 | 2022-06-28 | Application of fructus amomi essential oil in improving quality of paper mulberry silage |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115024393A true CN115024393A (en) | 2022-09-09 |
Family
ID=83126150
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210742830.6A Pending CN115024393A (en) | 2022-06-28 | 2022-06-28 | Application of fructus amomi essential oil in improving quality of paper mulberry silage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115024393A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115918787A (en) * | 2023-02-07 | 2023-04-07 | 西北农林科技大学 | Forage grass ensiling method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041289A (en) * | 2017-12-15 | 2018-05-18 | 湖北省农业科学院畜牧兽医研究所 | A kind of paper mulberry special ensiling additive and application |
| CN109845896A (en) * | 2019-01-02 | 2019-06-07 | 中国农业大学 | A kind of high protein roughage hybridization paper mulberry ensiling and preparation method thereof |
| CN109938176A (en) * | 2019-03-28 | 2019-06-28 | 黑龙江八一农垦大学 | A kind of method of clover high-quality ensiling |
| CN112167449A (en) * | 2020-11-06 | 2021-01-05 | 江西省科学院生物资源研究所 | Hybrid broussonetia papyrifera silage, preparation method, coarse feed and ruminant feed |
| CN113397039A (en) * | 2021-06-15 | 2021-09-17 | 大理白族自治州畜牧工作站 | Hybrid paper mulberry silage and processing method thereof |
-
2022
- 2022-06-28 CN CN202210742830.6A patent/CN115024393A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041289A (en) * | 2017-12-15 | 2018-05-18 | 湖北省农业科学院畜牧兽医研究所 | A kind of paper mulberry special ensiling additive and application |
| CN109845896A (en) * | 2019-01-02 | 2019-06-07 | 中国农业大学 | A kind of high protein roughage hybridization paper mulberry ensiling and preparation method thereof |
| CN109938176A (en) * | 2019-03-28 | 2019-06-28 | 黑龙江八一农垦大学 | A kind of method of clover high-quality ensiling |
| CN112167449A (en) * | 2020-11-06 | 2021-01-05 | 江西省科学院生物资源研究所 | Hybrid broussonetia papyrifera silage, preparation method, coarse feed and ruminant feed |
| CN113397039A (en) * | 2021-06-15 | 2021-09-17 | 大理白族自治州畜牧工作站 | Hybrid paper mulberry silage and processing method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 孙建政;孙国君;王晓娜;沈思军;李劼: "松针精油对苜蓿青贮饲料营养成分和发酵品质的影响", 绿洲农业科学与工程 * |
| 孙建政;王晓娜;孙国君;沈思军;: "松针精油对青贮饲料中主要微生物的影响", 饲料工业 * |
| 陈德奎: "砂仁精油对紫花苜蓿青贮品质的影响", 草地学报 * |
| 陈玉连: "砂仁精油对构树青贮品质的影响", 中国畜牧杂志 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115918787A (en) * | 2023-02-07 | 2023-04-07 | 西北农林科技大学 | Forage grass ensiling method |
| CN115918787B (en) * | 2023-02-07 | 2024-05-03 | 西北农林科技大学 | Forage grass silaging method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102864095B (en) | Lactobacillus plantarum used for silage alfalfa and use method thereof | |
| CN110692812A (en) | Raw material of solid feed for hermetia illucens | |
| CN104531566A (en) | Silage inoculant and application thereof | |
| CN106937704A (en) | A kind of composite probiotics ferment pleurotus eryngii edible fungi residue feed and its production method | |
| CN108835393B (en) | Mixed fermentation type mulberry branch and leaf micro-storage feed and preparation method thereof | |
| CN105941872A (en) | Lactobacillus buchneri inoculant as well as preparation method and application thereof | |
| CN109452449A (en) | A kind of method and its application of rapeseed meal raw material secondary fermentation production fermentation rapeseed meal | |
| CN111000046A (en) | Additive suitable for direct silage of high-moisture forage grass and preparation method thereof | |
| CN105851501A (en) | Mixed ensilage and preparation method thereof | |
| CN108576406A (en) | A kind of Silaging method of G. gracilis herbage | |
| CN110353087A (en) | The preparation method and fermented feed of rape stalk fermented feed | |
| CN112970952A (en) | Special fermentation auxiliary material and feed for ruminants and preparation method | |
| CN110982739B (en) | Lactobacillus plantarum for high-moisture oat silage and application thereof | |
| CN112544787A (en) | Method for fermenting paper mulberry compound feed by bacteria and enzyme in synergy mode and compound feed thereof | |
| CN115024393A (en) | Application of fructus amomi essential oil in improving quality of paper mulberry silage | |
| EP1438901A1 (en) | Silage additive and a process for preparing silage using it | |
| CN111887343A (en) | Preparation method of fermented mulberry leaves | |
| CN115812844A (en) | Fermented feed based on whole spent grains and preparation method thereof | |
| CN112335778B (en) | Fermented sweet sorghum TMR feed and preparation method thereof | |
| CN117378704A (en) | Method for producing biological feed by fermenting corncob with microorganism | |
| CN115024394A (en) | Application of formic acid in improving quality of broussonetia papyrifera ensilage | |
| CN107927353B (en) | Lactobacillus inoculant suitable for ensiling of myriophyllum viridis as well as preparation method and application thereof | |
| CN105901283A (en) | Method for rapid fermentation preparation of silage | |
| CN115462447A (en) | Method for preparing sorghum straw whole-plant corn mixed storage forage grass | |
| CN109452479A (en) | A kind of method that high-moisture corn fermentation generates people's pannage |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220909 |
|
| RJ01 | Rejection of invention patent application after publication |