CN101932925A - 用于从图像中除去自身荧光的方法和系统 - Google Patents
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
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- Immunology (AREA)
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- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (22)
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US11/948,205 US8031924B2 (en) | 2007-11-30 | 2007-11-30 | Methods and systems for removing autofluorescence from images |
PCT/EP2008/066189 WO2009068546A1 (en) | 2007-11-30 | 2008-11-26 | Methods and systems for removing autofluorescence from images |
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CN101932925A true CN101932925A (zh) | 2010-12-29 |
CN101932925B CN101932925B (zh) | 2013-11-20 |
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US (1) | US8031924B2 (zh) |
EP (1) | EP2212677B1 (zh) |
JP (1) | JP5496906B2 (zh) |
CN (1) | CN101932925B (zh) |
AU (1) | AU2008328861B2 (zh) |
CA (1) | CA2706428C (zh) |
WO (1) | WO2009068546A1 (zh) |
Cited By (3)
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---|---|---|---|---|
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CN111208106A (zh) * | 2013-12-16 | 2020-05-29 | 克罗姆尼贡公司 | 显微镜系统和用显微镜系统检测从样本发射的荧光的方法 |
CN113424050A (zh) * | 2019-02-13 | 2021-09-21 | 文塔纳医疗系统公司 | 用于计算多通道图像中的自体荧光贡献的系统和方法 |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2904691B1 (fr) * | 2006-08-02 | 2009-03-06 | Commissariat Energie Atomique | Procede et dispositif de reconstruction 3d de la distribution d'elements fluorescents. |
US8031924B2 (en) * | 2007-11-30 | 2011-10-04 | General Electric Company | Methods and systems for removing autofluorescence from images |
CA3162577C (en) | 2008-05-20 | 2023-09-26 | University Health Network | Device and method for fluorescence-based imaging and monitoring |
FR2950431B1 (fr) | 2009-09-24 | 2011-12-09 | Commissariat Energie Atomique | Dispositif et procede de reconstruction spatiale d'une cartographie de fluorescence |
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US8532398B2 (en) * | 2010-03-26 | 2013-09-10 | General Electric Company | Methods and apparatus for optical segmentation of biological samples |
EP2601513B1 (en) * | 2010-08-05 | 2014-05-14 | Cambridge Research & Instrumentation, Inc. | Enhancing visual assessment of samples |
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US11378517B2 (en) * | 2010-08-31 | 2022-07-05 | Konica Minolta, Inc. | Biological substance detection method |
US8818069B2 (en) * | 2010-11-30 | 2014-08-26 | General Electric Company | Methods for scaling images to differing exposure times |
FR2968921B1 (fr) | 2010-12-15 | 2013-01-11 | Commissariat Energie Atomique | Procede de localisation d'un marqueur optique dans un milieu diffusant |
US8977331B2 (en) | 2012-12-13 | 2015-03-10 | General Electric Company | Systems and methods for nerve imaging |
EP2963672A1 (en) | 2014-06-30 | 2016-01-06 | FEI Company | Computational scanning microscopy with improved resolution |
PL3171765T3 (pl) | 2014-07-24 | 2022-01-03 | University Health Network | Zbieranie i analiza danych do celów diagnostycznych |
JP6543347B2 (ja) * | 2015-02-06 | 2019-07-10 | ライフ テクノロジーズ コーポレーション | 光学的関心領域を測定する方法およびシステム |
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WO2016144341A1 (en) * | 2015-03-11 | 2016-09-15 | Siemens Aktiengesellschaft | Systems and methods for deconvolutional network based classification of cellular images and videos |
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US10827911B2 (en) * | 2016-06-03 | 2020-11-10 | Trustees Of Boston University | Optical imaging system employing vortex fiber for multiple-mode illumination |
BR112022001579A2 (pt) * | 2019-07-31 | 2022-04-19 | Somalogic Inc | Método, aparelho, e meio legível por computador para normalização adaptativa de níveis de analito |
CN114981642A (zh) * | 2020-02-14 | 2022-08-30 | 深圳华大智造科技股份有限公司 | 基于图像分析液滴的方法、计算机装置及存储介质 |
Family Cites Families (35)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH03152444A (ja) * | 1989-11-10 | 1991-06-28 | Kirin Brewery Co Ltd | 微生物の検出法および検出装置 |
JPH08334466A (ja) | 1995-06-06 | 1996-12-17 | Nikon Corp | 蛍光測光方法及び装置 |
US5880473A (en) | 1997-07-28 | 1999-03-09 | Applied Imaging, Inc. | Multifluor-fluorescence in-situ hybridization (M-FISH) imaging techniques using multiple multiband filters with image registration |
AU4920297A (en) * | 1996-10-25 | 1998-05-15 | Applied Imaging, Inc. | Multifluor-fluorescence in situ hybridization (m-fish) imaging techniques using multiple multiband filters with image registration |
US5902239A (en) * | 1996-10-30 | 1999-05-11 | U.S. Philips Corporation | Image guided surgery system including a unit for transforming patient positions to image positions |
CN1289239A (zh) * | 1998-01-26 | 2001-03-28 | 麻省理工学院 | 荧光成象内窥镜 |
US6990221B2 (en) * | 1998-02-07 | 2006-01-24 | Biodiscovery, Inc. | Automated DNA array image segmentation and analysis |
KR100691043B1 (ko) * | 1998-05-09 | 2007-03-09 | 아이코니시스 인코포레이티드 | 컴퓨터 제어된 태아 세포 포함 희귀 세포 진단 방법 및 장치 |
US6343228B1 (en) * | 1999-10-19 | 2002-01-29 | The Hong Kong University Of Science And Technology | Method and apparatus for fluorescence imaging of tissue |
US7187810B2 (en) * | 1999-12-15 | 2007-03-06 | Medispectra, Inc. | Methods and systems for correcting image misalignment |
JP2001198080A (ja) * | 2000-01-20 | 2001-07-24 | Fuji Photo Film Co Ltd | 蛍光画像表示方法および装置 |
JP2001258837A (ja) * | 2000-03-16 | 2001-09-25 | Fuji Photo Film Co Ltd | 蛍光観察装置 |
AU2001249386A1 (en) * | 2000-03-22 | 2001-10-03 | Quantum Dot Corporation | Methods of using semiconductor nanocrystals in bead-based nucleic acid assays |
US6748259B1 (en) * | 2000-06-15 | 2004-06-08 | Spectros Corporation | Optical imaging of induced signals in vivo under ambient light conditions |
US6980674B2 (en) * | 2000-09-01 | 2005-12-27 | Large Scale Proteomics Corp. | Reference database |
US6678398B2 (en) * | 2000-09-18 | 2004-01-13 | Sti Medical Systems, Inc. | Dual mode real-time screening and rapid full-area, selective-spectral, remote imaging and analysis device and process |
US6599694B2 (en) * | 2000-12-18 | 2003-07-29 | Cytokinetics, Inc. | Method of characterizing potential therapeutics by determining cell-cell interactions |
US7219016B2 (en) * | 2001-04-20 | 2007-05-15 | Yale University | Systems and methods for automated analysis of cells and tissues |
WO2003100069A1 (de) * | 2002-05-23 | 2003-12-04 | Koenig Karsten | Verfahren zum transfer von molekülen in vitale zellen mittels laserstrahlung sowie anordnung zur durchführung des verfahrens |
US6800249B2 (en) * | 2002-06-14 | 2004-10-05 | Chromavision Medical Systems, Inc. | Automated slide staining apparatus |
US7616985B2 (en) * | 2002-07-16 | 2009-11-10 | Xenogen Corporation | Method and apparatus for 3-D imaging of internal light sources |
US7668351B1 (en) * | 2003-01-17 | 2010-02-23 | Kestrel Corporation | System and method for automation of morphological segmentation of bio-images |
US20040225222A1 (en) * | 2003-05-08 | 2004-11-11 | Haishan Zeng | Real-time contemporaneous multimodal imaging and spectroscopy uses thereof |
US7321791B2 (en) * | 2003-09-23 | 2008-01-22 | Cambridge Research And Instrumentation, Inc. | Spectral imaging of deep tissue |
JP2005287964A (ja) * | 2004-04-02 | 2005-10-20 | Olympus Corp | 生体・臓器・組織の観察装置 |
JP4790248B2 (ja) * | 2004-03-31 | 2011-10-12 | オリンパス株式会社 | 観察装置および蛍光観察装置 |
US7589839B2 (en) | 2004-03-31 | 2009-09-15 | Olympus Corporation | Examination apparatus, fluoroscopy apparatus, examination method, and experimental method |
EP2191774A3 (en) * | 2004-12-06 | 2010-06-23 | Cambridge Research & Instrumentation, Inc. | Systems and methods for in-vivo optical imaging and measurement |
US7831075B2 (en) * | 2005-10-20 | 2010-11-09 | Case Western Reserve University | Imaging system |
US8078265B2 (en) * | 2006-07-11 | 2011-12-13 | The General Hospital Corporation | Systems and methods for generating fluorescent light images |
US8131476B2 (en) * | 2006-08-07 | 2012-03-06 | General Electric Company | System and method for co-registering multi-channel images of a tissue micro array |
US8244021B2 (en) * | 2006-12-20 | 2012-08-14 | Ventana Medical Systems, Inc. | Quantitative, multispectral image analysis of tissue specimens stained with quantum dots |
US8031924B2 (en) * | 2007-11-30 | 2011-10-04 | General Electric Company | Methods and systems for removing autofluorescence from images |
US8135187B2 (en) * | 2008-03-26 | 2012-03-13 | General Electric Company | Method and apparatus for removing tissue autofluorescence |
US20100121172A1 (en) * | 2008-11-12 | 2010-05-13 | Siemens Corporate Research, Inc. | Microscopic and macroscopic data fusion for biomedical imaging |
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Cited By (6)
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CN111208106A (zh) * | 2013-12-16 | 2020-05-29 | 克罗姆尼贡公司 | 显微镜系统和用显微镜系统检测从样本发射的荧光的方法 |
US11668918B2 (en) | 2013-12-16 | 2023-06-06 | Kromnigon Ab | System and method for fluorescence microscopy with detection of light emission from multiple fluorochromes |
CN106018355A (zh) * | 2015-03-27 | 2016-10-12 | 希森美康株式会社 | 待测样品分析方法及待测样品分析装置 |
CN106018355B (zh) * | 2015-03-27 | 2019-01-18 | 希森美康株式会社 | 待测样品分析方法及待测样品分析装置 |
CN113424050A (zh) * | 2019-02-13 | 2021-09-21 | 文塔纳医疗系统公司 | 用于计算多通道图像中的自体荧光贡献的系统和方法 |
CN113424050B (zh) * | 2019-02-13 | 2025-01-10 | 文塔纳医疗系统公司 | 用于计算多通道图像中的自体荧光贡献的系统和方法 |
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CA2706428A1 (en) | 2009-06-04 |
JP2011505004A (ja) | 2011-02-17 |
WO2009068546A1 (en) | 2009-06-04 |
EP2212677B1 (en) | 2019-10-16 |
US8031924B2 (en) | 2011-10-04 |
US20090141959A1 (en) | 2009-06-04 |
JP5496906B2 (ja) | 2014-05-21 |
CN101932925B (zh) | 2013-11-20 |
AU2008328861B2 (en) | 2014-07-10 |
AU2008328861A1 (en) | 2009-06-04 |
CA2706428C (en) | 2016-05-24 |
EP2212677A1 (en) | 2010-08-04 |
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