CN101919407A - Biological suspension pesticide prepared by compounding abamectin with bacillus thuringiensis - Google Patents
Biological suspension pesticide prepared by compounding abamectin with bacillus thuringiensis Download PDFInfo
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- CN101919407A CN101919407A CN 201010235259 CN201010235259A CN101919407A CN 101919407 A CN101919407 A CN 101919407A CN 201010235259 CN201010235259 CN 201010235259 CN 201010235259 A CN201010235259 A CN 201010235259A CN 101919407 A CN101919407 A CN 101919407A
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- bacillus thuringiensis
- avermectin
- biological suspension
- suspension pesticide
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Abstract
The invention relates to a biological suspension pesticide prepared by compounding abamectin with bacillus thuringiensis, which comprises 88-93 percent by weight of main agent and 7-12 percent by weight of auxiliary agent, wherein the main agent is a mixture of the abamectin and the bacillus thuringiensis; the auxiliary agent comprises a dispersing agent, a thickening agent and other assistants; and the dispersing agent is polyoxyalkylene ether FS1 and urea and magnesium aluminum silicate. According to the formula, the biological suspension pesticide is prepared by the steps of: adding a bacillus thuringiensis fermentation liquor, the abamectin, the urea, the magnesium aluminum silicate and the thickening agent into a ball-milling reactive kettle, stirring at low speed, adding the FS1, and stirring at high speed; and then adding the other assistants for stirring uniformly to obtain the biological suspension pesticide. In the biological suspension pesticide, the content of the abamectin is not less than 2 percent, the content of insecticidal crystal protein of the bacillus thuringiensis is kept above 80 percent, the survival rate of the bacillus thuringiensis spore is above 90 percent, and the cotoxicity coefficient of the pesticide is more than 120. The invention is beneficial to delaying the generation of pesticide resistance of insects, and has the advantages of quick effect-taking, long valid period and low cost lower than or not more than that of the traditional similar pesticides.
Description
Technical field
The invention belongs to a kind of compound biotic insecticide technical field of killing lepidoptera pest, specifically, relate to a kind of bacillus thuringiensis and abamectin compounded suspension pesticide.
Technical background
Lepidoptera pest is the main and important pests on the agricultural, and the damage to crops scope is wide, and the extent of injury is big, is the importance of agricultural production to the control of this class pest always.That generally uses at present both at home and abroad has chemical pesticide and a biopesticide, wherein chemical pesticide in the highest flight, biopesticide accounts for 2%, mainly contains bacillus thuringiensis, Avermectin, pleocidin etc.
Bacillus thuringiensis (Bacillus thuringiensis, abbreviation Bt) this bacterium is the Gram-positive agrobacterium, belong to bacillus, the insecticidal proteins of its generation and gemma have very high insecticidal activity, be used for preventing and treating diamond-back moth, cotton bollworm, snout moth's larva etc.Because its unique mechanism of action, to mammality and environmental friendliness, Bt at home and abroad obtains to use widely in the decades in past, has a good application prospect.Brood cell and crystalline protein active insecticidal components exist easy inactivation under the ultraviolet irradiation, insecticidal spectrum is narrow, insecticidal rate is low weakness but Bt lives, and the brood cell that lives is difficult for long-term preservation, and shelf life is short, limits its large-scale commercial.Avermectin (Avermectin) is a kind of 16 membered macrolide compounds, has efficient insecticide, kills mite, nematocide activity.Be a kind of new antibiotic class medicament, because its novel chemical constitution and mechanism of action, to human and livestock safety, extensive use on producing in recent years is used for the control of crop pests, evil mite in a large number.But along with applying on a large scale of Avermectin, insect has been developed into a major issue to the pesticide resistance of Avermectin.
Because bacillus thuringiensis has the weakness that insecticidal spectrum is narrow, insecticidal rate is low, and Avermectin toxicity is stronger, insect easily develops immunity to drugs.If the Bt zymotic fluid is mixed composite with Avermectin, just may produce the insecticidal rate height, insecticidal spectrum is wide, toxicity is low, pest resistance to insecticide produces slow neoformation agricultural chemicals.Studies show that, Bt and Avermectin composite, and selection proper auxiliary agent, not only can overcome two kinds of medicine unfavorable factors, improve stability, the prolongation shelf life of Bt, reduce the decomposition and the drug-fast generation of Avermectin, also have significant synergistic effect, provide an approach with the improvement of safety effectively and reasonably for insect.But composite bacillus thuringiensiss of adopting solid fermentations of present bacillus thuringiensis and Avermectin more, the former powder of A Wei is directly admixed, and Ah's dimension hplc of admixing is generally at 0.1-0.2%, because the Abamectin water-soluble extreme difference, content is low again, and composite back effect is undesirable.
Summary of the invention
Adopt the bacillus thuringiensis of solid fermentation directly to mix the unfavorable problem of composite effect in order to solve prior art, the invention provides the composite biological suspension pesticide of a kind of Avermectin and bacillus thuringiensis with the former powder of A Wei.
The technical solution that realizes above-mentioned purpose is as follows:
The composite biological suspension pesticide of Avermectin and bacillus thuringiensis is made up of host and assistant agent, and percentage by weight is host 88-93%, assistant agent 7-12%;
Described host is that the bacillus thuringiensis of toxicity evaluation 2000 above international unit/milligrams and purity are the mixture of 95% Avermectin, and its weight ratio is bacillus thuringiensis: Avermectin=25-45: 1;
Described assistant agent is dispersant, thickener and other assistant agents;
Described dispersant is polyoxy alkane alkene ether FS1 and urea and the U.S. aluminium of silicic acid;
By above-mentioned prescription, take by weighing bacillus thuringiensis zymotic fluid, Avermectin, urea, aluminium-magnesium silicate and thickener, and add together in the ball-milling reaction still, stirring at low speed 30 minutes, add Chinese (FS1), high-speed stirred 2 hours adds other assistant agent then and stirs evenly, and promptly gets the complex biological suspension pesticide of Avermectin and bacillus thuringiensis.
The percentage by weight of dispersant, thickener, other assistant agents is respectively 6.2-6.9%, 1.5-1.6%, 0.1-3.3% in the described assistant agent.
The percentage by weight of polyoxy alkane alkene ether FS1, urea, the U.S. aluminium of silicic acid is respectively 4.0-4.5%, 1.0%, 1.2-1.4% in the described dispersant.
Described thickener is organobentonite and xanthans, and the percentage by weight of organobentonite and xanthans is respectively 1.4-1.5%, 0.1%.
Described other assistant agent is meant preservative or synergist or its mixture, is specially three-tree butyl paracresol, salicylic acid lipid, benzoic acid lipid, imidazolidine ketone.
Compared with prior art, complex biological suspension pesticide of the present invention has following useful result:
(1) have synergistic effect, composite dose co-toxicity coefficient is greater than 120.
(2) help delaying pest resistance to insecticide and produce, the insect of antagonism Avermectin is effective; And rapid-action, the term of validity is long.
(3) price is lower than or is not higher than existing same insecticides.
(4) make mix preparation after, Avermectin content is not less than 2%, the insecticidal crystal protein content of bacillus thuringiensis keeps more than 80%, the survival rate of gemma is more than 90%.
Embodiment
Below in conjunction with embodiment, the present invention is done to describe further.
Embodiment 1:
The preparation of 2% A Wei and the composite suspending agent of 2700IU Bt
The mass ratio of each component is: toxicity evaluation is the bacillus thuringiensis zymotic fluid 90% of 3000IU/mg, purity is 95% Avermectin 2.2%, assistant agent is by the dispersant polyoxy alkane alkene ether FS14% of this grease production of bamboo, urea 1%, aluminium-magnesium silicate 1.2%, organobentonite 1.4%, xanthans 0.1%, three-tree butyl paracresol 0.1% is common industrial chemicals.
Compound method: by above-mentioned prescription, the an amount of bacillus thuringiensis zymotic fluid of weighing, Avermectin, urea, aluminium-magnesium silicate, organobentonite and the good xanthans of swelling add in the ball-milling reaction still together, after the stirring at low speed 30 minutes, add polyoxy alkane alkene ether FS1, high-speed stirred 2 hours, add three-tree butyl paracresol then and stir evenly, promptly get the composite biological suspension pesticide of Avermectin and bacillus thuringiensis.
After preparation was finished, complex biological suspension pesticide physics, chemical property character were as follows:
The outward appearance thick liquid that is creamy white, going into water dispersible is top grade, cloud disperses;
Suspensibility reaches more than 85%;
Particle diameter 15% is less than 1um, and 85% less than 10um, and average grain diameter is less than 4um;
Pourability is qualified, and toppling over residual volume is 0.8%, and toppling over residual volume after the washing is 0.1%.
The active ingredient Avermectin reaches more than 90% of addition;
Su Yun Jin Jing body protein content keeps more than 80%;
Su Yun gold gemma survival rate is more than 85%.
Embodiment 2:
The preparation of the composite suspending agent of 3% A Wei 2500IU Bt
The mass ratio of each component is: toxicity evaluation is the bacillus thuringiensis zymotic fluid 85% of 3000IU/mg, purity is 95% Avermectin 3.2%, assistant agent polyoxy alkane alkene ether FS14.5%, urea 1%, aluminium-magnesium silicate 1.4%, organobentonite 1.5%, xanthans 0.1%, three-tree butyl paracresol 0.1%, benzoate 3.2%.
Compound method: by above-mentioned prescription, the an amount of bacillus thuringiensis zymotic fluid of weighing, Avermectin, urea, aluminium-magnesium silicate, benzoate, organobentonite and the good xanthans of swelling add in the ball-milling reaction still together, after the stirring at low speed 30 minutes, add polyoxy alkane alkene ether FS1, high-speed stirred 2 hours, add three-tree butyl paracresol then and stir evenly, promptly get the composite biological suspension pesticide of Avermectin and bacillus thuringiensis.
After preparation was finished, complex biological suspension pesticide physics, chemical property character were as follows:
The outward appearance thick liquid that is creamy white, going into water dispersible is top grade, cloud disperses;
Suspensibility reaches more than 85%;
Particle diameter 15% is less than 1um, and 85% less than 10um, and average grain diameter is less than 4um;
Pourability is qualified, and toppling over residual volume is 0.8%, and toppling over residual volume after the washing is 0.1%.
The active ingredient Avermectin reaches more than 90% of addition;
Su Yun Jin Jing body protein content keeps more than 80%;
Su Yun gold gemma survival rate is more than 85%.
Embodiment 3:
Press the Toxicity Determination of the suspending agent of embodiment 1 preparation to cotton bollworm
During Toxicity Determination, use worm with cotton bollworm do test in 3 ages, adopt infusion process to carry out the virulence test, repeat 4 times, 4 days effect behind the investigation medicine, the calculation correction lethality, ask the drug concentration logarithm--the regression equation of-lethality probability value, and the comptibility test of the side of card, obtain LC50 then, calculate the co-toxicity coefficient (CTC) of relative virus force index (TI) and intermixture with reference to the abundant method of Sun Yun.Its result of the test such as table 1 when bacillus thuringiensis and Avermectin are mixed, have synergistic effect.
Table 1 bacillus thuringiensis Avermectin is to the Toxicity Determination of cotton bollworm (behind the medicine 4 days)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | TI | CTC |
| Bacillus thuringiensis | Y＝4.23+4.92X | 0.955 | 1.032，1.07～4.32 | 100.00 | |
| Avermectin | Y＝17.40+4.44X | 0.977 | 0.106，0.97～4.76 | 1935.00 | |
| Complex preparation | Y＝10.8900+3.75X | 0.958 | 0.029，0.99～4.65 | 2900.00 | 142.07 |
Claims (5)
1. the composite biological suspension pesticide of Avermectin and bacillus thuringiensis, it is characterized in that: be made up of host and assistant agent, percentage by weight is host 88-93%, assistant agent 7-12%;
Described host is that the bacillus thuringiensis of toxicity evaluation 2000 above international unit/milligrams and purity are the mixture of 95% Avermectin, and its weight ratio is bacillus thuringiensis: Avermectin=25-45: 1;
Described assistant agent is dispersant, thickener and other assistant agents;
Described dispersant is polyoxy alkane alkene ether FS1 and urea and the U.S. aluminium of silicic acid;
By above-mentioned prescription, take by weighing bacillus thuringiensis zymotic fluid, Avermectin, urea, aluminium-magnesium silicate and thickener, and add together in the ball-milling reaction still, stirring at low speed 30 minutes, add Chinese (FS1), high-speed stirred 2 hours adds other assistant agent then and stirs evenly, and promptly gets the composite biological suspension pesticide of Avermectin and bacillus thuringiensis.
2. Avermectin according to claim 1 and the composite biological suspension pesticide of bacillus thuringiensis, it is characterized in that: the percentage by weight of dispersant, thickener, other assistant agents is respectively 6.2-6.9%, 1.5-1.6%, 0.1-3.3% in the described assistant agent.
3. Avermectin according to claim 1 and the composite biological suspension pesticide of bacillus thuringiensis is characterized in that: the percentage by weight of polyoxy alkane alkene ether FS1, urea, the U.S. aluminium of silicic acid is respectively 4.0-4.5%, 1.0%, 1.2-1.4% in the described dispersant.
4. Avermectin according to claim 1 and the composite biological suspension pesticide of bacillus thuringiensis, it is characterized in that: described thickener is organobentonite and xanthans, and the percentage by weight of organobentonite and xanthans is respectively 1.4-1.5%, 0.1%.
5. Avermectin according to claim 1 and the composite biological suspension pesticide of bacillus thuringiensis, it is characterized in that: described other assistant agent is meant preservative or synergist or its mixture, is specially three-tree butyl paracresol, salicylic acid lipid, benzoic acid lipid, the imidazolidine ketone.
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| CN 201010235259 CN101919407A (en) | 2010-07-21 | 2010-07-21 | Biological suspension pesticide prepared by compounding abamectin with bacillus thuringiensis |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102047915A (en) * | 2011-01-11 | 2011-05-11 | 青岛海利尔药业有限公司 | Insecticidal composition containing thiacloprid amide |
| CN104788196A (en) * | 2015-04-16 | 2015-07-22 | 王龙潮 | Replanting-resistant compound yield-increasing agent |
| CN104996495A (en) * | 2014-08-07 | 2015-10-28 | 太仓市永发农场专业合作社 | Efficient biopesticide applied in controlling insect diseases for high-quality paddy rice and production method thereof |
| US20170290339A1 (en) * | 2014-09-17 | 2017-10-12 | Bayer Cropscience Lp | Compositions comprising recombinant bacillus cells and an insecticide |
| CN107361083A (en) * | 2017-08-18 | 2017-11-21 | 马鞍山中粮生物化学有限公司 | A kind of efficient pesticides and preparation method thereof |
| CN107417775A (en) * | 2017-09-21 | 2017-12-01 | 福建省农业科学院农业生物资源研究所 | A kind of Bt Pesticidal toxins of AVM coupling and its application |
| CN108208015A (en) * | 2018-02-08 | 2018-06-29 | 武汉楚强生物科技有限公司 | A kind of agricultural chemicals suspension agent and its preparation method and application |
| US10779542B2 (en) | 2013-03-15 | 2020-09-22 | Spogen Biotech Inc. | Fusion proteins and methods for stimulating plant growth, protecting plants, and immobilizing bacillus spores on plants |
| US10836800B2 (en) | 2014-09-17 | 2020-11-17 | Spogen Biotech Inc. | Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria |
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| CN1293899A (en) * | 1999-11-02 | 2001-05-09 | 福建省农业科学院生物技术中心 | Physically multi-point coupled biological insecticide |
| CN1421139A (en) * | 2002-05-28 | 2003-06-04 | 张用梅 | Recompounded biological farm pesticide and the prepn of its suspension and wettable powder |
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| CN1293899A (en) * | 1999-11-02 | 2001-05-09 | 福建省农业科学院生物技术中心 | Physically multi-point coupled biological insecticide |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102047915A (en) * | 2011-01-11 | 2011-05-11 | 青岛海利尔药业有限公司 | Insecticidal composition containing thiacloprid amide |
| US11882829B2 (en) | 2013-03-15 | 2024-01-30 | Spogen Biotech Inc. | Fusion proteins and methods for stimulating plant growth, protecting plants, and immobilizing bacillus spores on plants |
| US10779542B2 (en) | 2013-03-15 | 2020-09-22 | Spogen Biotech Inc. | Fusion proteins and methods for stimulating plant growth, protecting plants, and immobilizing bacillus spores on plants |
| US11134681B2 (en) | 2013-03-15 | 2021-10-05 | Spogen Biotech Inc. | Fusion proteins and methods for stimulating plant growth, protecting plants from pathogens, and immobilizing Bacillus spores on plant roots |
| CN104996495A (en) * | 2014-08-07 | 2015-10-28 | 太仓市永发农场专业合作社 | Efficient biopesticide applied in controlling insect diseases for high-quality paddy rice and production method thereof |
| US20220151240A1 (en) * | 2014-09-17 | 2022-05-19 | Bayer Cropscience Lp | Compositions comprising recombinant bacillus cells and an insecticide |
| US20170290339A1 (en) * | 2014-09-17 | 2017-10-12 | Bayer Cropscience Lp | Compositions comprising recombinant bacillus cells and an insecticide |
| US11905315B2 (en) | 2014-09-17 | 2024-02-20 | Spogen Biotech Inc. | Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria |
| US10836800B2 (en) | 2014-09-17 | 2020-11-17 | Spogen Biotech Inc. | Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria |
| US11044916B2 (en) * | 2014-09-17 | 2021-06-29 | Bayer Cropscience Lp | Compositions comprising recombinant Bacillus cells and an insecticide |
| CN104788196A (en) * | 2015-04-16 | 2015-07-22 | 王龙潮 | Replanting-resistant compound yield-increasing agent |
| CN107361083A (en) * | 2017-08-18 | 2017-11-21 | 马鞍山中粮生物化学有限公司 | A kind of efficient pesticides and preparation method thereof |
| CN107417775B (en) * | 2017-09-21 | 2021-03-30 | 福建省农业科学院农业生物资源研究所 | Abamectin-coupled Bt insecticidal toxin and application thereof |
| CN107417775A (en) * | 2017-09-21 | 2017-12-01 | 福建省农业科学院农业生物资源研究所 | A kind of Bt Pesticidal toxins of AVM coupling and its application |
| CN108208015A (en) * | 2018-02-08 | 2018-06-29 | 武汉楚强生物科技有限公司 | A kind of agricultural chemicals suspension agent and its preparation method and application |
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Application publication date: 20101222 |