CN111109295B - Pesticide composition with kasugamycin and nano-silver as active components and application thereof - Google Patents
Pesticide composition with kasugamycin and nano-silver as active components and application thereof Download PDFInfo
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- CN111109295B CN111109295B CN202010045852.8A CN202010045852A CN111109295B CN 111109295 B CN111109295 B CN 111109295B CN 202010045852 A CN202010045852 A CN 202010045852A CN 111109295 B CN111109295 B CN 111109295B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a pesticide composition taking kasugamycin and nano-silver as active components and application thereof, wherein the pesticide composition comprises the active components of kasugamycin and nano-silver in a mass ratio of 100: 1-1: 100. the kasugamycin and the nano silver used in the invention have obvious synergistic effect after being compounded.
Description
Technical Field
The invention belongs to the technical field of pesticide compounding, and particularly relates to a pesticide composition taking kasugamycin and nano-silver as active components and application thereof.
Background
Citrus canker is a bacterial disease caused by the pathogen citrus canker. The citrus canker pathogen is Flavobacterium carpoformomonas xanthomonas citri pathogenic variety of the Leptomonas melegueta of the phylum LeptomonasXanthomonas axonopodis pv. citriThe citrus canker pathogen can infect various rutaceae plants, and is most susceptible to diseases of common sweet oranges, navel oranges, sour oranges and the like. The germs can be transmitted along with splashed raindrops and high-speed wind, and penetrate through skin holes, stomata and wounds to invade plants, so that leaves, fruits and stem parts are in crater scabs, and immature fruits are cracked, deformed, dropped and fallen leaves are caused. The existing medicines for preventing and treating citrus canker pathogenic bacteria mainly take copper preparation single agents and compound preparations as main materials, such as copper hydroxide, copper oxychloride, kasugao, copper oxychloride, copper zineb, copper calcium sulfate, basic copper sulfate, kasuga, oxine-copper, mesogen copper acetate, copper rosinate, cuaminosulfate, copper succinate and the like. But copper formulationsHas certain limitations of poor crop safety, poor mixability or serious drug resistance and the like. Therefore, the development of drugs or compound combinations with high biological activity on citrus canker is urgent.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides the pesticide composition taking kasugamycin and nano-silver as active components and the application thereof.
The invention adopts the following technical scheme that a pesticide composition with kasugamycin and nano-silver as active components comprises the active components of kasugamycin and nano-silver, wherein the mass percentage of the kasugamycin to the nano-silver is 100: 1-1: 100.
further, the mass percentage of kasugamycin to nano silver is preferably 10: 1-1: 10. within this range, the synergistic effect is most pronounced.
Further, in the pesticide composition, the mass sum of the active ingredients kasugamycin and the silver particles accounts for 1.5-60% of the total mass of the pesticide composition.
Further, in the pesticide composition, the mass sum of the active components kasugamycin and the silver particles accounts for 3-40% of the total mass of the pesticide composition.
Furthermore, the pesticide composition also contains auxiliary components, wherein the auxiliary components include but are not limited to fillers, auxiliaries and water.
The invention also provides a wettable pesticide powder composition taking kasugamycin and nano-silver as active components, which comprises the following components in percentage by mass: 1-60% of kasugamycin, 0.1-10% of nano silver, 2-10% of dispersing agent, 2-10% of wetting agent and the balance of filler to 100%.
Further, the preparation method of the pesticide wettable powder composition comprises the following steps: proportionally putting the kasugamycin active compound, the nano-silver active compound, the dispersing agent, the wetting agent and the filler into a mixer for mixing, crushing by a jet mill, and uniformly mixing to obtain the kasugamycin nano-silver nano-particles.
The invention also provides a pesticide suspending agent composition taking kasugamycin and nano-silver as active components, which comprises the following components in percentage by mass: 1-60% of kasugamycin, 0.1-10% of nano silver, 2-10% of dispersing agent, 0.1-3% of defoaming agent, 0.05-3% of thickening agent, 0.05-9% of antifreezing agent and water until the water content is 100%.
Further, the preparation method of the pesticide suspending agent composition comprises the following steps: the technical grade of kasugamycin, the nano silver, the dispersing agent, the defoaming agent, the thickening agent and the antifreezing agent in the formula are sequentially added into water, and the pH of the liquid is regulated and controlled within the range of 3.5-5.0 by hydrochloric acid in the whole processing process, so that the kasugamycin is obtained.
The adjuvants and fillers used in the composition of the present invention are common to those skilled in the art, and belong to auxiliary components that can be added to pesticides. According to different formulations, the composition can also be added with dispersing agents, wetting agents, thickening agents, defoaming agents, antifreezing agents, fillers and other auxiliary agents commonly used by the technical personnel in the field.
Further, the wetting agent is selected from: fatty alcohol-polyoxyethylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, sodium laurate sulfate, sodium dodecyl sulfate, nekal, tween 60 and the like.
Further, the dispersant is selected from: sodium lignosulfonate, MF, sodium methylenedinaphthalene sulfonate, saponin powder, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether, ammonium sulfate, succinic acid and the like.
Further, the thickener is selected from: starch, corn flour, carboxymethyl cellulose, gelatin, and the like.
Further, the anti-freezing agent is selected from: propylene glycol, glycerin, ethylene glycol, diethylene glycol, and the like.
Further, the filler is selected from one or more of kaolin, talcum powder, white carbon black, light calcium carbonate, diatomite, bentonite, argil, attapulgite, montmorillonite, mica powder, perlite, calcite, illite, pyrophyllite, water and castor oil.
The invention also protects the application of the pesticide composition, the pesticide wettable powder composition and the pesticide suspending agent composition in inhibiting citrus canker pathogen.
The pesticide composition is generally used by adopting a spraying method, and other application technologies can be adopted according to the actual agricultural production requirements.
Compared with the prior art, the invention has the beneficial effects that:
1. the bacteriostatic mechanism of kasugamycin is mainly to prevent the initiation of protein translation (Tanaka N, 1965), thereby affecting protein synthesis and causing bacterial death; the chemical structure of silver determines that the silver has higher catalytic capability, and the reduction potential of Ag ions is extremely high enough to enable the surrounding space to generate active oxygen which can sterilize. Meanwhile, when the Ag ions contact with bacteria, the Ag ions immediately permeate into cells or are kept on cell membranes, so that the Ag ions have the effects of blocking the growth and synthesis path of the bacteria and blocking an energy system, and the denaturation of enzyme protein and the biological damage of the cell membranes are caused, thereby killing the bacteria. When the thallus is killed by Ag ions, the Ag ions are dissociated from the bacterial corpses and contact with other bacterial colonies, and the above-mentioned actions are repeated. At present, the silver particles are considered to be adhered to the outer membrane of the bacteria and then penetrate through the outer membrane to damage the bacteria so as to achieve the sterilization effect. The two active components have different action mechanisms, and the two active components are compounded for use in the invention, thereby obtaining the technical effect of being beneficial to slowing down the generation of drug resistance of pathogenic bacteria.
2. The pesticide composition has a very obvious synergistic effect on citrus canker pathogens through the synergistic effect of kasugamycin and nano-silver.
Detailed Description
The present invention is further illustrated in the following examples, which do not limit the scope of the invention.
Example 1
Indoor Activity assay
Indoor toxicity determination of kasugamycin and nano-silver compounded 50% water dispersible granules on citrus canker
Test subjects: the citrus canker pathogen is from Lipeboshi laboratory of Guizhou Kaili institute of Guizhou southeast national medicine comprehensive utilization engineering research center.
The test method comprises the following steps: the bacteriostatic activity of the kasugamycin and silver particle composition 50% water dispersible granules on citrus canker pathogenic bacteria is measured by a turbidity method.
Preparing the composition into drug-containing NB culture medium with different concentration gradients, and measuring OD of the 5 concentrations of drug-containing sterile NB culture medium595And the value is the OD value of the sterile medium. Respectively adding the bacterial suspension, performing shake culture in a constant temperature shaking table at 28 deg.C and 180 rpm/min for 48 h, and measuring OD of bacterial liquid with each concentration on enzyme labeling instrument595The value is the OD value of the culture medium containing bacteria, a virulence curve is drawn by adopting a double dilution method, and EC is obtained50(mean effective concentration) value.
Corrected OD value-bacteria-containing medium OD value-sterile medium OD value
Control effect (OD value of corrected contrast culture medium bacterial liquid-corrected toxic culture medium OD value)/corrected contrast culture medium bacterial liquid OD value
The synergistic effect of the combination of fungicides was evaluated according to the Wadley method.
EC50 theory=（a+b）/（a/ EC50A+b/ EC50B）
SR= EC50 theory/ EC50 practice
A and B represent the pharmaceutical components, respectively; a and b are respectively the content ratio of each component in the compound combination. When SR is less than or equal to 0.5, antagonism is obtained; 0.5 < SR < 1.5 is additive; SR is more than or equal to 1.5, which is the synergistic effect.
The results of the indoor virulence determination are shown in table 1 below:
TABLE 1 indoor toxicity test results of kasugamycin and nano silver compounding on citrus canker pathogen
The indoor toxicity test result shows that the ratio of kasugamycin to nano silver is 100: 1-1: 100, has bacteriostasis and synergism on citrus canker germs, and particularly has the bactericidal synergism effect on citrus canker germs within 10: 1-1: between 10, the SR is more than 1.65 (including 1.65), which shows that the synergy is more remarkable.
The pesticide composition can be prepared into any one dosage form suitable for agriculture by a known method, and the better dosage forms are water dispersible granules and suspending agents.
In order to make the object and technical solution of the present invention clearer, the following specific examples are given for illustration, but not for limitation.
Example 2: 30% kasugamycin and nano-silver wettable powder
Composition ratio of kasugamycin and nano-silver wettable powder with 230 percent of surface
The active ingredients, various auxiliary agents, fillers and the like are uniformly mixed according to the proportion shown in the table 2, and the mixture is crushed by a superfine crusher to obtain the 30 percent kasugamycin-nano silver wettable powder.
Example 3: 10% kasugamycin-nano silver suspending agent
TABLE 310% kasugamycin-nano silver dry suspending agent component ratio
The active components of kasugamycin technical, the active components of nano-silver, sodium lignosulfonate, carboxymethyl cellulose, an organic silicon compound and glycerin which are shown in the formula in the table 3 are sequentially added into water, and the pH value is adjusted to 3.5-5.0 by hydrochloric acid, so that the 10% kasugamycin-nano-silver suspending agent can be obtained.
Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (3)
1. The pesticide composition with kasugamycin and nano-silver as active components is characterized by comprising the following active components in percentage by mass: 1-1: 10, the sum of the mass of the active ingredients of kasugamycin and the mass of the nano silver accounts for 3 to 40 percent of the total mass of the pesticide composition.
2. The pesticide composition as set forth in claim 1, wherein the pesticide composition further comprises auxiliary components, including but not limited to fillers and adjuvants.
3. Use of a pesticidal composition according to claim 1 or 2 for the control of citrus canker.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010045852.8A CN111109295B (en) | 2020-01-16 | 2020-01-16 | Pesticide composition with kasugamycin and nano-silver as active components and application thereof |
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| CN202010045852.8A CN111109295B (en) | 2020-01-16 | 2020-01-16 | Pesticide composition with kasugamycin and nano-silver as active components and application thereof |
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| CN111109295A CN111109295A (en) | 2020-05-08 |
| CN111109295B true CN111109295B (en) | 2021-07-27 |
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| CN112640896B (en) * | 2020-12-25 | 2022-05-17 | 中国农业大学 | Surface-modified silver nano suspending agent for preventing and treating pine wood nematode disease and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106889066A (en) * | 2017-02-14 | 2017-06-27 | 陕西康禾立丰生物科技药业有限公司 | A kind of agricultural chemicals of the kasugarnycin of high content |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106889066A (en) * | 2017-02-14 | 2017-06-27 | 陕西康禾立丰生物科技药业有限公司 | A kind of agricultural chemicals of the kasugarnycin of high content |
Non-Patent Citations (2)
| Title |
|---|
| 纳米银制备及提高生防菌剂抗菌活性;马汇泉等;《农药》;20110430;第50卷(第4期);第258-260页 * |
| 纳米银材料对植物病原细菌抑制作用的研究;甘林;《中国优秀硕士论文全文数据库 工程科技 Ⅰ辑》;20120215;B020-235 * |
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