CN1293899A - Physically multi-point coupled biological insecticide - Google Patents

Physically multi-point coupled biological insecticide Download PDF

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Publication number
CN1293899A
CN1293899A CN 99122187 CN99122187A CN1293899A CN 1293899 A CN1293899 A CN 1293899A CN 99122187 CN99122187 CN 99122187 CN 99122187 A CN99122187 A CN 99122187A CN 1293899 A CN1293899 A CN 1293899A
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bacillus thuringiensis
avermectin
point coupled
coupled biological
biological insecticide
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CN 99122187
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CN1167345C (en
Inventor
刘波
林国宪
林融初
林抗美
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BIOLOGICAL TECH CENTER FUJIAN PROVINCIAL ACADEMY OF AGRICULTURAL SCIENCES
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BIOLOGICAL TECH CENTER FUJIAN PROVINCIAL ACADEMY OF AGRICULTURAL SCIENCES
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Priority to CNB991221877A priority Critical patent/CN1167345C/en
Publication of CN1293899A publication Critical patent/CN1293899A/en
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Abstract

A bioinsecticide is prepared from bacillus thuringiesis and abamectin through mixing and features very high insecticiding effect.

Description

Physically multi-point coupled biological insecticide
The present invention relates to a kind of insecticide that contains microorganism, particularly a kind of physically multi-point coupled biological insecticide.
Although bacillus thuringiensis has no residual hazard, free from environmental pollution, advantage such as use cost is low as biopesticide, also exist the narrow and insecticidal rate of insecticidal spectrum simultaneously and wait weak point slowly.The antiinsect antibiotic Avermectin has high-efficiency broad spectrum but the distinguishing feature of production cost costliness.
The objective of the invention is to overcome weak point of the prior art, and a kind of high-efficiency broad spectrum, no residual hazard, free from environmental pollution are provided, simultaneously the lower again physically multi-point coupled biological insecticide of use cost.
Of the present inventionly realize by following approach simultaneously.
Physically multi-point coupled biological insecticide comprises bacillus thuringiensis, and its composition main points are: also include Avermectin, bacillus thuringiensis mixes with Avermectin.
Avermectin also is a kind of biological insecticides, it has high-efficiency broad spectrum but characteristics such as production cost costliness, these characteristics characteristics lucky and bacillus thuringiensis form good complementarity, thereby make the present invention have high-efficiency broad spectrum, no residual hazard, free from environmental pollution, characteristics such as simultaneously use cost is lower again.
Because bacillus thuringiensis is a kind of stomach toxicity type insecticide, Avermectin then is the malicious type insecticide of a kind of nerve, thus the two mix insecticide also produced complement each other, this unexpected effect of phase mutual excitation synergy.
What is more important, the combination of two kinds or two or more toxin has produced this notion of multidigit point desinsection toxin.As everyone knows, various toxalbumin all have corresponding with it acceptor and action site, and a kind of toxin protein has been formed pesticide resistance, only be that variation has taken place the corresponding a kind of acceptor of toxalbumin therewith, and other acceptor do not become.Do not become the corresponding toxin protein of acceptor with this and still bring into play insecticidal action, the reason that does not produce cross resistance between the dissimilar toxin proteins is because acceptor and the action site that has separately.Compare with single toxin, multidigit point desinsection toxin more helps Pest Control, prevents the pest resistance that extensively takes place.
The bacillus thuringiensis English name is: Bacillus thuringiensis, and the Avermectin English name is: Abamectin.Therefore the inventor is called for short the present invention and is the BtA biological insecticides.Avermectin is a kind of macrolide antiinsect antibiotic, and domestic major product has that the outstanding worm of collection completely restrains, A Bading etc.
Purpose of the present invention can also realize by following approach.
Volumetric ratio between bacillus thuringiensis (16000IU tires) and the abamectin solution (containing 1.8% Avermectin) is 1: 0.0002~0.001.
Though any volumetric ratio between bacillus thuringiensis and the Avermectin all has above-mentioned effect,, mix according to above-mentioned volumetric ratio, then can obtain better effect, its reason comes from the inventor and has following location parameter:
The location parameter of the ratio combination that bacillus thuringiensis is different with Avermectin:
In the table: Bt is bacillus thuringiensis (wetting powder, 16000IU tires)
A is abamectin solution (containing 1.8% Avermectin)
LC50 is the lethal dose of 50
Bt is the ratio of volume with the ratio of A
The examination worm is the instar larvae of diamond-back moth
Volumetric ratio between bacillus thuringiensis (16000IU tires) and the abamectin solution (containing 1.8% Avermectin) is 1: 0.0006.
Just can know in fact from last table, the slope maximum of handling D (promptly 1: 0.0006) is 2.11, slope B value is used to illustrate that insect colony is to handling the reaction uniformity and the susceptibility of this drug regimen of D, be that more insect individuality is to handling all even sensitivity of reaction of D, in conjunction with co-toxicity coefficient and synergy multiple and production cost comprehensive distinguishing, handling D is best complex proportioning (promptly 1: 0.0006).
The abamectin solution of the bacillus thuringiensis of dust cloud state and mist of oil state is adsorbed mutually and the physics lotus root is closed.
Bacillus thuringiensis mixes with Avermectin, can adopt to stir and wait some usual manners to carry out.But owing to bacillus thuringiensis is aqueous is aqua, Avermectin is aqueous then to be finish, in the recombination process of the two, it is uneven even differ greatly to exist between former medicine and filler and the raw material relative density, the improper uneven problem of the mutual physical distribution of former medicine that causes of this hybrid mode, when dusting operation in the field, can cause former pharmaceutically active ingredient excessive, and influence the compound action of former pharmaceutically active ingredient in the separation in space.Bacillus thuringiensis and Avermectin are in that the two adsorbs the physics mat mutually and closes resulting product of the present invention and have characteristics such as physical distribution is even between the former medicine under the mist attitude.
The adsorbing base that also comprises a kind of powder shape, the bacillus thuringiensis solution of water smoke state and the abamectin solution of mist of oil state are adsorbed on the adsorbing base and physical coupling jointly.
Adsorbing base is the potassium dihydrogen phosphate pulvis.
In sum, the present invention has following advantage compared to existing technology: not only combine bacillus thuringiensis and Avermectin advantage separately, make the present invention have advantages such as low, the no residual hazard of use cost, free from environmental pollution, desinsection spectrum width and killing rate be fast simultaneously, its pesticidal applications scope has comprised more than 90% of pest, and insecticidal spectrum expands to five orders---Lepidoptera, Homoptera, coleoptera, diptera and mite class.And the compound use of the bacillus thuringiensis of stomach toxicity type and the Avermectin of neural malicious type has also produced complementary synergy on insecticidal effect.Killing rate at 12,18,24,36,48 hours is respectively 81.2%, 86.6%, 91.8%, 95%, 100%, its insecticidal rate>85%/24 hour.The more important thing is, the present invention proposes this notion of multidigit point desinsection toxin, it prevents the extensively pest resistance of generation for more helping Pest Control, makes at present limited biological insecticides research find new life that a kind of brand-new, effective product is provided.
Below we carry out more detailed description to the present invention in conjunction with the embodiments.
Embodiment 1:
Physically multi-point coupled biological insecticide, comprise bacillus thuringiensis pulvis and abamectin solution, bacillus thuringiensis mixes with abamectin solution, the proportioning of the two is 1: 0.0002, wherein, bacillus thuringiensis is tired and is 16000IU, and abamectin solution contains 1.8% Avermectin.
Embodiment 2:
Physically multi-point coupled biological insecticide, comprise bacillus thuringiensis pulvis and abamectin solution, the Avermectin of the bacillus thuringiensis of dust cloud state and mist of oil state is adsorbed and physical coupling mutually, the proportioning of bacillus thuringiensis and abamectin solution is 1: 0.001 (volumetric ratio), wherein, bacillus thuringiensis is tired and is 16000IU, and abamectin solution contains 1.8% Avermectin.Most preferred embodiment:
Physically multi-point coupled biological insecticide, comprise bacillus thuringiensis solution and abamectin solution and adsorbing base, under 60 ℃ of temperature, the Avermectin of the bacillus thuringiensis of water smoke state and mist of oil state is adsorbed on the adsorbing base jointly, the proportioning of bacillus thuringiensis and Avermectin and adsorbing base is 1: 0.0006: (volumetric ratio), wherein, bacillus thuringiensis is tired and is the bacillus solution of 16000IU, abamectin solution contains 1.8% Avermectin, and adsorbing base is a phosphoric acid dioxy potassium pulvis.

Claims (6)

1. physically multi-point coupled biological insecticide comprises bacillus thuringiensis, it is characterized in that, also includes Avermectin, and bacillus thuringiensis mixes with Avermectin.
2. physically multi-point coupled biological insecticide according to claim 1 is characterized in that, bacillus thuringiensis (16000IU tires) is 1: 0.0002~0.001 (volumetric ratio) with the proportioning of abamectin solution (containing 1.8% Avermectin).
3. physically multi-point coupled biological insecticide according to claim 1 is characterized in that, bacillus thuringiensis (16000IU tires) is 1: 0.0006 (volumetric ratio) with the proportioning of abamectin solution (containing 1.8% Avermectin).
4. physically multi-point coupled biological insecticide according to claim 1 is characterized in that, the abamectin solution of the bacillus thuringiensis of dust cloud state and mist of oil state is adsorbed mutually and the physics lotus root is closed.
5. physically multi-point coupled biological insecticide according to claim 1, it is characterized in that, the adsorbing base that also comprises a kind of powder shape, the bacillus thuringiensis solution of water smoke state and the abamectin solution of mist of oil state are adsorbed on the adsorbing base and physical coupling jointly.
6. physically multi-point coupled biological insecticide according to claim 5 is characterized in that, adsorbing base is the potassium dihydrogen phosphate pulvis.
CNB991221877A 1999-11-02 1999-11-02 Physically multi-point coupled biological insecticide Expired - Fee Related CN1167345C (en)

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CN1167345C CN1167345C (en) 2004-09-22

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101919407A (en) * 2010-07-21 2010-12-22 合肥工业大学 Biological suspension pesticide prepared by compounding abamectin with bacillus thuringiensis
CN104996495A (en) * 2014-08-07 2015-10-28 太仓市永发农场专业合作社 Efficient biopesticide applied in controlling insect diseases for high-quality paddy rice and production method thereof
CN107474121A (en) * 2017-09-21 2017-12-15 福建省农业科学院农业生物资源研究所 A kind of Bt Pesticidal toxins of AVM coupling and its application

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101919407A (en) * 2010-07-21 2010-12-22 合肥工业大学 Biological suspension pesticide prepared by compounding abamectin with bacillus thuringiensis
CN104996495A (en) * 2014-08-07 2015-10-28 太仓市永发农场专业合作社 Efficient biopesticide applied in controlling insect diseases for high-quality paddy rice and production method thereof
CN107474121A (en) * 2017-09-21 2017-12-15 福建省农业科学院农业生物资源研究所 A kind of Bt Pesticidal toxins of AVM coupling and its application
CN107474121B (en) * 2017-09-21 2021-04-09 福建省农业科学院农业生物资源研究所 Abamectin-coupled Bt insecticidal toxin and application thereof

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