CN101427691B - Scarabaeus green muscardine fungus and green muscardine fungus native mixed insecticidal composition - Google Patents
Scarabaeus green muscardine fungus and green muscardine fungus native mixed insecticidal composition Download PDFInfo
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- CN101427691B CN101427691B CN2008102199921A CN200810219992A CN101427691B CN 101427691 B CN101427691 B CN 101427691B CN 2008102199921 A CN2008102199921 A CN 2008102199921A CN 200810219992 A CN200810219992 A CN 200810219992A CN 101427691 B CN101427691 B CN 101427691B
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- destruxins
- metarhizium anisopliae
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- green muscardine
- insecticidal composition
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Abstract
The invention discloses a mixed disinsection composition of Metarhizium anisopliae and destruxins; wherein the ratio of the number of Metarhizium anisopliae conidium to the mg number of destruxins is (0.1-10.0)*10<6>:10-100. The mixed disinsection composition conquers the defects of the prior disinsection composition that the Metarhizium anisopliae is singly used to controlling soil insects with unstable control effect and slow effect. Using synergism of destruxins to Metarhizium anisopliae, after a large amount of tests, the best mixing ratio of the Metarhizium anisopliae to destruxins is determined to develop the mixed disinsection composition of Metarhizium anisopliae and destruxins, at the same time the insecticide containing the mixed disinsection composition is provided. The preparation and use method of the insecticide are simple and the technical proposal is feasible, with stable and obvious control efficiency and great value to control the soil insects especially white grub.
Description
Technical field
The invention belongs to biological pesticide technical field, be specifically related to a kind of Metarhizium anisopliae and destruxins synergistic blend Pesticidal combination.
Background technology
Metarhizium anisopliae (Metarhizium anisopliae) is a kind of important insect pathogenic fungus, insects such as coleoptera, Lepidoptera, Hymenoptera, Homoptera, diptera there is infection ability, particularly underground insect (as grub) there is control action preferably, utilizes that green muscardine fungus control of grubs class is for example big to wait gill cockchafer etc. to have more than 100 year history.Yet, use the Metarhizium anisopliae pest control separately, its control efficiency instability, and it is slow to take effect.The compounded pesticides of exploitation Metarhizium anisopliae overcomes the unstable and slow defective that takes effect of its control efficiency, to control soil insect especially grub category insect, has great importance.
Destruxins (destruxin) is an insect pathogenic fungus---the cyclic depsipeptides antibiotic that green muscardine fungus Metarhiziumanisopliae var.anisopliae produces.Destruxins has significant insecticidal effect, and has the brand-new mechanism of action that is different from existing insecticide, mainly acts on the hemolymph immune system of insect, makes it to lose the immune defense ability.Destruxins is easily degraded, noresidue, and toxicity is low, and the compounded pesticides of development and use destruxins has important practical significance for overcoming pest resistance to insecticide and improving control efficiency.
Summary of the invention
An object of the present invention is to overcome independent use green muscardine fungus pest control control efficiency instability, the slow defective that takes effect, a kind of Metarhizium anisopliae and destruxins synergistic blend Pesticidal combination are provided.
Purpose of the present invention is achieved by following technical proposals:
The invention provides a kind of Metarhizium anisopliae and destruxins mixed insecticidal composition, described Metarhizium anisopliae conidium mixes in following ratio with destruxins: Metarhizium anisopliae conidium (individual): destruxins (milligram)=(0.1～10.0) * 10
6: (10～100).
The preferred Metarhizium anisopliae conidium of described ratio (individual): destruxins (milligram)=(0.5～5.0) * 10
6: (20～60).
Described Metarhizium anisopliae conidium is the conidium that survives.
Described destruxins can be any or their the two or more mixtures that mix with any ratio among destruxins A, B, C, D, the E.
Pesticidal combination of the present invention can be processed into formulations such as finish, suspension emulsion, granula, pulvis, wetting powder or microcapsule formulations.
The invention has the beneficial effects as follows: the present invention has overcome that existing control soil insect is used its control efficiency instability of green muscardine fungus separately, slow deficiency takes effect, utilize the synergistic effect of destruxins to green muscardine fungus, by a large amount of experimental studies, the mixed proportion that the two is best has been determined in selection, develop green muscardine fungus and the destruxins Pesticidal combination that is mixed, the insecticide that contains described composition is provided simultaneously.Insecticide preparation of the present invention and using method are simple, and technical scheme is practical, and control efficiency is stable and remarkable, to control soil insect especially grub, have great importance.
Embodiment
Further describe the present invention below in conjunction with specific embodiment.
Embodiment 1: the intermixture of Metarhizium anisopliae MaQ10 bacterial strain conidium and destruxins is to the Toxicity Determination of gill cockchafer Exolontha serrulata (Gyllenhall) such as big
Experiment
Described Metarhizium anisopliae MaQ10 bacterial strain has detailed argumentation in application number is 200810219405.9 patent application.Described destruxins can be any or their the two or more mixtures that mix with any ratio among destruxins A, B, C, D, the E.
Big gill cockchafer 1 instar larvae that waits adopts infusion process to measure green muscardine fungus conidium, destruxins and both mixtures virulence of tagging to grub for examination.Earlier conidial powder is dissolved in the 0.02% Tween 80 aqueous solution, stirs, be mixed with 10
7, 5 * 10
6, 10
6, 5 * 10
5, 10
5The medicament of a series of concentration of individual conidium/ml, again with destruxins by 100,20,10 and 60mg/L join respectively in the above-mentioned microbial inoculum, contrast is 0.01% Tween 80 solution.To soak 10s at soup or contrast in the liquid for the examination grub, and put into plastic cup after drying, dress 100g is through the soil of sterilization in the cup, and 1 of every glass of foster worm is put into some grain germination wheats as feed in the cup, raises down at 25 ℃.Each handles 10 grubs, repeats 3 times (30), and same experiment repeats 2 times.Every the 24h check result.Write down dead borer population, adopt the DPS statistical software to analyze experimental result, ask virulence equation and half lethal concentration LC
50And half lethal time LT
50
The result shows that the destruxins of adding 20 and 60mg/L in conidial suspension can significantly improve the big lethality that waits gill cockchafer.Part of test results sees Table 1; Simultaneously, its half lethal concentration and half lethal time also obviously diminish, and see Table 2, illustrate that destruxins mixes the preventive and therapeutic effect that can significantly improve grub with the Metarhizium anisopliae conidium, shorten the control responding time.
Table 1 Metarhizium anisopliae MaQ10 bacterial strain conidium and destruxins are to the lethal effect result of gill cockchafer such as big
*There are not significant difference (multiple range test, DMRT) (p＜0.05) between the data that letter is identical in the same column
Table 2MaQ10 bacterial strain conidium and destruxins are to the LC of gill cockchafer such as big
50And LT
50And confidence interval
Embodiment 2: the intermixture of Metarhizium anisopliae MaB08 bacterial strain conidium and destruxins is to the Toxicity Determination experiment of Brontispa longissima Brontispa longissima
Described Metarhizium anisopliae MaB08 bacterial strain has detailed argumentation in application number is 200810219404.4 patent application.
With the 0.02% Tween 80 aqueous solution conidium is mixed with the series concentration spore suspension, again with destruxins A (DA) by 60 and 20mg/L add respectively, contrast is 0.02% Tween 80 solution.Destruxins A also can use any or their the two or more mixtures that mix with any ratio among destruxins B, C, D, the E to replace, and adds respectively by total amount 60 and 20mg/L.
With the Poter spray tower spore suspension and contrast liquid are sprayed on on the examination Brontispa longissima 2 instar larvae bodies, will trys worm after drying and change the dependent insect cage worm over to and raise with the cocoanut tree lobus cardiacus, each handles 10 examination worms, repeats 3 times (30), tests repetition 2 times equally.Every the 24h check result.Write down the borer population of dying of illness, adopt the DPS statistical software to analyze experimental result, ask virulence equation and half lethal concentration LC
50And half lethal time LT
50
The result shows, in conidial suspension, add 20 and destruxins A, the B of 60mg/L, C, D, E in any, can significantly improve the lethality of Brontispa longissima.With destruxins A (DA) is example, and experimental result sees Table 3; Simultaneously, its half lethal concentration and half lethal time also obviously diminish, and see Table 4, illustrate that destruxins mixes the control efficiency that can significantly improve Brontispa longissima with the Metarhizium anisopliae conidium.
Table 3MaB08 bacterial strain conidium and destruxins are to the lethal effect of Brontispa longissima
*There are not significant difference (multiple range test, DMRT) (p＜0.05) between the data that letter is identical in the same column
Table 4MaB08 bacterial strain conidium and destruxins are to the LC of Brontispa longissima
50And LT
50And confidence interval
Claims (5)
1. Metarhizium anisopliae and destruxins mixed insecticidal composition is characterized in that described Metarhizium anisopliae conidium mixes in following ratio with destruxins: the milligram number of the conidial number of Metarhizium anisopliae and destruxins is than being (0.1～10.0) * 10
6: 10～100.
2. according to described Metarhizium anisopliae of claim 1 and destruxins mixed insecticidal composition, it is characterized in that the conidial number of described Metarhizium anisopliae and the milligram number ratio of destruxins are (0.5～5.0) * 10
6: 20～60.
3. according to described Metarhizium anisopliae of claim 1 and destruxins mixed insecticidal composition, it is characterized in that described Metarhizium anisopliae conidium is the conidium that survives.
4. according to described Metarhizium anisopliae of claim 1 and destruxins mixed insecticidal composition, it is characterized in that described destruxins can be any or their the two or more mixtures that mix with any ratio among destruxins A, B, C, D, the E.
5. according to described Metarhizium anisopliae of claim 1 and destruxins mixed insecticidal composition, it is characterized in that described mixed insecticidal composition is processed into finish, suspension emulsion, granula, pulvis, wetting powder or microcapsule formulations.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102199921A CN101427691B (en) | 2008-12-15 | 2008-12-15 | Scarabaeus green muscardine fungus and green muscardine fungus native mixed insecticidal composition |
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|---|---|---|---|
| CN2008102199921A CN101427691B (en) | 2008-12-15 | 2008-12-15 | Scarabaeus green muscardine fungus and green muscardine fungus native mixed insecticidal composition |
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| CN101427691A CN101427691A (en) | 2009-05-13 |
| CN101427691B true CN101427691B (en) | 2011-11-16 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004888B (en) * | 2012-05-16 | 2014-07-30 | 中国农业科学院植物保护研究所 | Metarrhizium anisopliae granular preparation and preparation method thereof |
| CN110226607B (en) * | 2019-07-26 | 2021-02-09 | 山东省农业科学院植物保护研究所 | Medicament composition for preventing and treating cucumber aphid and cucumber bacterial angular leaf spot |
| CN113475532A (en) * | 2021-07-28 | 2021-10-08 | 广州毅田生物技术有限公司 | Metarhizium anisopliae compound biological insecticide and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2008175A1 (en) * | 1990-01-19 | 1991-07-19 | Jalpa P. Tewari | Herbicide for cruciferous weeds |
| CN101002573A (en) * | 2006-12-26 | 2007-07-25 | 华南农业大学 | Compounding insecticide containing destruxin and abamectin |
| CN101016331A (en) * | 2006-12-26 | 2007-08-15 | 华南农业大学 | Method of separating and purifying destruxins of Metarrhizium anisopliae |
| CN101560473A (en) * | 2008-11-25 | 2009-10-21 | 华南农业大学 | Strain with high destruxins yield and application thereof |
-
2008
- 2008-12-15 CN CN2008102199921A patent/CN101427691B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2008175A1 (en) * | 1990-01-19 | 1991-07-19 | Jalpa P. Tewari | Herbicide for cruciferous weeds |
| CN101002573A (en) * | 2006-12-26 | 2007-07-25 | 华南农业大学 | Compounding insecticide containing destruxin and abamectin |
| CN101016331A (en) * | 2006-12-26 | 2007-08-15 | 华南农业大学 | Method of separating and purifying destruxins of Metarrhizium anisopliae |
| CN101560473A (en) * | 2008-11-25 | 2009-10-21 | 华南农业大学 | Strain with high destruxins yield and application thereof |
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