CN110066739A - A kind of muscardine SB010 and its application in the common setose thistle Malaysia and China of prevention and treatment - Google Patents
A kind of muscardine SB010 and its application in the common setose thistle Malaysia and China of prevention and treatment Download PDFInfo
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- CN110066739A CN110066739A CN201910337089.3A CN201910337089A CN110066739A CN 110066739 A CN110066739 A CN 110066739A CN 201910337089 A CN201910337089 A CN 201910337089A CN 110066739 A CN110066739 A CN 110066739A
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- thrips
- bacterial strain
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- spore
- muscardine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
Abstract
The present invention discloses a kind of application of muscardine bacterial strain SB010 in prevention and treatment thrips, wherein the bacterial strain SB010 is preserved in Guangdong Province's Culture Collection on April 19th, 2018, deposit number is GDMCC NO:60359.Common big thrips population can be effectively suppressed in the bacterial strain SB010, and to people, animal, plant and Environmental security, can partially substitute thrips chemoprevention pesticide, have very big application potential in biological control.
Description
Technical field
The present invention relates to plant protection arts, and in particular to field of biological control more particularly to muscardine and its
The application for preventing and treating common setose thistle Malaysia and China.
Background technique
Chemical pesticide plays great function in control of insect, and the usage amount and yield of China's pesticide occupy the world for a long time
It is the first.However according to statistics, the effective rate of utilization of global pesticide is less than 30%, and the annual chemical pesticide usable floor area in China is 2.8 hundred million
hm2More than, amount of application reaches ten thousand tons of 50-60, and about 80% chemical pesticide is directly entered environment, and (Li Liang, Zhang Zhanying, Sun Huiying wait
Pesticide harm and environment protective plant protecting Discussion on Technology [J] Hubei plant protection, 2018, (4): 63-64).As chemical pesticide is a large amount of for a long time
It uses, bring " 3R " problem, that is, pest resistance to insecticide (Resistance), pesticide residue (Residue) and pest run wild again
(Resurgence) and problem of environmental pollution is increasingly severe.Therefore, to change using the tradition prevention and treatment based on chemical pesticide
Mode implements the plant protection policy of " putting prevention first, integrated control ", adheres to based on cultural control, based on biological control, and combine
Be extremely important (Yang Yunhua, Du Kai are developed in the sustainable Jiankang that other control measures carry out comprehensive treatment to pest
Book, Research Progress on Biological Control [J] Henan Science and Technology College journal of Shi Mingwang entomogenous fungi, 2011,39 (1): 34-37).
Thrips is the general designation of Thysanoptera (Thysanoptera) Thripidae (Thripida) insect.Thrips class pest figure is micro-
It is small, it is easy to hide, and reproductive capacity is strong, growth cycle is short, and generation overlap is serious, increases difficulty for prevention and treatment.Thrips body is small or living
Move hidden, initial stage of causing harm is not easy to be discovered by people, is often just found after causing major disaster.Currently, the class pest is anti-
It controls and depends on chemical prevention, since the normal phase lacks ideal, efficient chemical agent, lead to the unreasonable of a large amount of chemical agents
Use, to cause pest resistance to insecticide, killing natural enemy, pesticide residue, the problems such as environmental pollution, Product quality and safety also by
To threat.In recent years, global trade and climate warming accelerate the diffusion and propagation of thrips, and thrips has become worldwide important
Agriculture and forestry injurious insect.So seeking environmentally friendly control strategy extremely urgent today, biological control is in the anti-of thrips class pest
It controls in work and has broad application prospects.In addition, biological control can ensure the quality safety of product, energy in process of production
The effective usage amount for reducing chemical agent, so far, the research about bio-control method prevention and treatment thrips pest are existing not
Understatement road.
Muscardine is subordinate to Deuteromycotina (Deutero mycotina), Moniliales (Moniliales), clump spore
Stalk section (moniliaceae), Beauveria (Beaveriaspp), is important biological and ecological methods to prevent plant disease, pests, and erosion strain.Muscardine is black in Mexico
It is found for the first time on locust (Melanopius mexicanus), the initial stage seventies, is applied to the gill in west May as biocontrol microorganisms for the first time
It in cockchafer, and is succeeded, attracts extensive attention.It is infected by force for example, disclosing one plant in yellow roc etc. and having to hot-zone flowers and fruits thrips
The beauveria bassiana BB-T02 of power is good to hot-zone flowers and fruits thrips tool to infect lethal effect (CN107384803A)；Li Yinping etc.
(2013) isolated to the efficient beauveria bassiana SZ-26 bacterial strain of Frankliniella occidentalis from the Ostrinia furnacalis worm corpse of Northeast China
(Chinese biological preventing and treating journal)；Yuan Shengyong etc. (2011 and 2013) is separated from three-spotted phytometra larvae to be obtained to Frankliniella occidentalis high poison
The beauveria bassiana MZ050724 bacterial strain of power, and to 080717 bacterial strain of beauveria bassiana Bb of the high virulence of Gynaikothrips uzeli and
MZ050724 bacterial strain (Southwest University's journal, environmental entomology report, Chinese forest disease pest)；Wang Haihong etc. is separated from corn borer and is obtained
Obtain two plants of beauveria bassianas (104263655 A of CN 104212724 A and CN) for having good control efficiency to Frankliniella occidentalis；It applies
Powerful army etc. (2016) has found one plant of beauveria bassiana (106135295 A of CN) for having good control efficiency to alfalfa thrips, etc.
Deng.Wherein as it can be seen that most study is Biocontrol Effect of the beauveria bassiana to thrips.
Summary of the invention
The purpose of the present invention is to provide to thrips, especially common setose thistle saddlery has the biocontrol microorganisms of excellent biocontrol effect.
In the course of the research, discovery is before this to the muscardine bacterial strain SB010 of prodenia litura certain effect to the tool of common big thrips
There is more prominent toxic effect.The patent application 201810596222.2 that muscardine bacterial strain SB010 is proposed in the applicant
It is identified in (insecticide and its application associated with a kind of matrine and muscardine), and is protected on April 19th, 2018
It is hidden in Guangdong Province's Culture Collection, deposit number is GDMCC NO:60359.
The present invention provides application of the bacterial strain in prevention and treatment thrips accordingly.Preferably, the thrips is common big thrips.
In one embodiment, spore liquid will be made after the strain culturing, containing the microbial inoculum of spore.Bacterial strain when application
Spore concentration is 107Spore/mL to 109Spore/mL, preferably 108Spore/mL to 109Spore/mL.
In addition, the bacterial strain and thrips can also be prevented and treated with other drug combinations, especially common big thrips, may obtain compared with
Good synergistic effect.
The present inventor's discovery in screening (candidate strain also includes some beauveria bassianas) is certain to prodenia litura before this
The muscardine bacterial strain SB010 of effect has special effect to common big thrips.The biological experiment of process shows this hair
Bright muscardine bacterial strain reaches 90% (with 10 to common big thrips control effect8Spore/mL is tested), and same item
It is only 36% under common muscardine effect under part；Under 0.4mg/ml concentration under the conditions of bacterial strain fermentation liquor, SB010 is not existing together
The reason time is all significantly higher than other bacterial strains to the lethality of thrips, and the 5th day corrected mortality is much higher than other bacterium up to 93.3%
Strain (see the experimental result and table of specific embodiment).Therefore, muscardine bacterial strain SB010 of the invention can be effectively suppressed general
Lead to big thrips population, and to people, animal, plant and Environmental security, can partially substitute thrips chemoprevention pesticide, have in biological control
Very big application potential.
Specific embodiment
It is introduced, but is not constituted to limit of the invention below by R&D process and specific embodiment of the invention
System.
Embodiment: the bioassay of bacterial strain
1 for trying insect
Common big thrips population picks up from Guangzhou Zhu Cun cowpea field, and in laboratory rearing mostly generation, rearing conditions are temperature 26
DEG C, relative humidity 65%, photoperiod L: D=12: 12.
2 strains testeds
List the bacterial strain that part is screened for:
SB009: beauveria bassiana (Beauveria bassiana) bacterium is preserved in Guangdong Province's Culture Collection
(GDMCC), deposit number is GDMCC NO:60588 (in addition proposing patent application 2019102239096).
SB010: bacterial strain muscardine (Beauveria brongniartii) is preserved in Guangdong Province microorganism fungus kind guarantor
Hiding center, deposit number be GDMCC NO:60359 (in addition proposition patent application 201810596222.2, which provide
Preservation information).
SP016: beauveria bassiana (Beauveria bassiana) is preserved in the biological control Ministry of Education, Agricultural University Of South China
Engineering Research Center.
SP535: fumosorosea (Isaria fumosorosea) is preserved in Agricultural University Of South China's biological control education
Portion's Engineering Research Center.
SP502: fumosorosea (Isaria fumosorosea) is preserved in Agricultural University Of South China's biological control education
Portion's Engineering Research Center.
MZ16: Metarhizium anisopliae (Metarhizium anisopliae) is preserved in Agricultural University Of South China's biological control
Ministry of Education's Engineering Research Center.
3 experimental methods
(1) preparation of spore suspension
On PDA plate after 26 ± 1 DEG C of culture 7d, 0.05% Tween-80 of entomogenous fungi conidium of spore is sufficiently produced
Sterile water elution spore is wiped with magnetic stirrer, then after being placed in 25 DEG C of shaken cultivation 25min of shaking table 180rpm with double-deck
The filtering of mirror paper, is counted with blood counting chamber, measures the concentration of mother liquor, be configured to 1 × 108Spore/mL spore suspension.
(2) preparation of bacterial strain fermentation liquor
Each bacterial strain 1 × 10 is prepared in aforementioned manners7Spore/mL spore suspension is inoculated with the prepared spore suspension of 5mL
Liquid in equipped with 50mL SDA culture medium conical flask in, 26 DEG C 180rpm shaken cultivation 5 days, Buchner funnel collected by suction filtrate,
It measures its protein concentration and is diluted to 0.4mg/ml.
(3) toxicity test of the entomogenous fungi to common big thrips
Prepared spore suspension and fermentation liquid are placed in flat finger-type pipe (15mm × 75mm), poured out after impregnating 2h
Spore suspension, it is spare after finger-type pipe natural air drying；Cowpea dissection (1cm, both ends are non-porous), immerses spore suspension, and 30s takes
Out, it is correspondingly placed into after natural air drying in the finger-type pipe handled well, while accessing common big thrips female adult pest 50, cotton sealing,
It is placed in growth cabinet, using 0.05% Tween-80 sterile water as blank control, each processing is repeated 4 times.Continuous 5d records it
The death rate.
(3) data processing
Test process analysis is carried out using 19.0 software of SPSS, each result is analyzed using one-way analysis of variance,
And use Tukey testing significance of difference.
4 experimental results
Experimental result shows, pathogenicity significant difference of the different strains spore suspension to common big thrips, bacterial strain SB010
It is significantly higher than other bacterial strains (table 1, table 2)；Same bacterial strain, various concentration, different disposal time pathogenicity have differences, common big
The death rate of thrips female adult pest rises with the increase of concentration and processing time, when concentration is 1 × 108(table when spore/mL
1), SB010 is 90% in the cumulative mortality of 5d, is significantly higher than concentration 1 × 107When spore/mL.
The result shows that bacterial strain SB010 has preferable lethal effect to common big thrips, belong to potential excellent biocontrol microorganisms.
Corrected mortality (%) of 1 different strains of table to common big thrips
Note: examining through Tukey, and the different lowercases of same row indicate pathogenicity significant difference (P < between different strains
0.05)
Corrected mortality (%) of 2 different strains of table to common big thrips
Note: examining through Tukey, and the different lowercases of same row indicate pathogenicity significant difference (P < between different strains
0.05)
Further, measurement bacterial strain fermentation liquor shows that SB010 is in the different disposal time pair to the experimental result of the virulence of thrips
The lethality of thrips is all significantly higher than other bacterial strains (being shown in Table 3), wherein under 0.4mg/ml concentration, the 5th day corrected mortality
Up to 93.3%, it is much higher than other bacterial strains.
Corrected mortality (%) of the 3 different strains fermentation liquid of table to common big thrips
Note: examining through Tukey, and the different lowercases of same row indicate pathogenicity significant difference (P < between different strains
0.05)。
Claims (6)
1. application of the muscardine bacterial strain SB010 in prevention and treatment thrips, wherein the bacterial strain SB010 was on April 19th, 2018
It is preserved in Guangdong Province's Culture Collection, deposit number is GDMCC NO:60359.
2. application as described in claim 1, which is characterized in that the thrips is common big thrips.
3. application as described in claim 1, which is characterized in that spore liquid will be made after bacterial strain SB010 culture, containing spore
Microbial inoculum.
4. application as claimed in claim 3, which is characterized in that the spore concentration of bacterial strain SB010 is 10 when application7 Spore/mL
To 109 Spore/mL.
5. application as claimed in claim 4, which is characterized in that spore concentration 108 Spore/mL to 109 Spore/mL.
6. such as application described in any one of claim 1 to 5, which is characterized in that the bacterial strain and the medicament of other prevention and treatment thrips join
It closes and uses.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910337089.3A CN110066739B (en) | 2019-04-25 | 2019-04-25 | Beauveria bassiana SB010 and application thereof in prevention and treatment of common thistle marigold |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910337089.3A CN110066739B (en) | 2019-04-25 | 2019-04-25 | Beauveria bassiana SB010 and application thereof in prevention and treatment of common thistle marigold |
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| CN110066739B CN110066739B (en) | 2021-08-17 |
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Cited By (7)
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| CN111084195A (en) * | 2019-12-12 | 2020-05-01 | 西北农林科技大学 | Aphid-killing microbial preparation and use methods thereof |
| CN111909854A (en) * | 2020-07-20 | 2020-11-10 | 华南农业大学 | Zero-valent iron-Beauveria brucei nano-particles and preparation method and application thereof |
| CN112715577A (en) * | 2020-10-22 | 2021-04-30 | 华南农业大学 | Application of matrine and beauveria bassiana in preventing and treating common circium japonicum |
| CN114317292A (en) * | 2022-01-30 | 2022-04-12 | 华南农业大学 | Space breeding beauveria bassiana strain SCAUHT12 with high pathogenicity to common thrips and application thereof |
| CN114317294A (en) * | 2022-01-30 | 2022-04-12 | 华南农业大学 | Spaceflight entomogenous fungus strain SCAUHT56 with high pathogenicity and high ultraviolet resistance to common thrips and application thereof |
| CN114317291A (en) * | 2022-01-30 | 2022-04-12 | 华南农业大学 | Space breeding beauveria bassiana strain SCAUHT21 with high pathogenicity to common thrips and application thereof |
| CN114317293A (en) * | 2022-01-30 | 2022-04-12 | 华南农业大学 | Spaceflight entomogenous fungus strain SCAUHT18 with high pathogenicity and high ultraviolet resistance to common thrips and application thereof |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111084195A (en) * | 2019-12-12 | 2020-05-01 | 西北农林科技大学 | Aphid-killing microbial preparation and use methods thereof |
| CN111909854A (en) * | 2020-07-20 | 2020-11-10 | 华南农业大学 | Zero-valent iron-Beauveria brucei nano-particles and preparation method and application thereof |
| CN111909854B (en) * | 2020-07-20 | 2023-02-03 | 华南农业大学 | Zero-valent iron-Beauveria bassiana nano-particles and preparation method and application thereof |
| CN112715577A (en) * | 2020-10-22 | 2021-04-30 | 华南农业大学 | Application of matrine and beauveria bassiana in preventing and treating common circium japonicum |
| CN114317292A (en) * | 2022-01-30 | 2022-04-12 | 华南农业大学 | Space breeding beauveria bassiana strain SCAUHT12 with high pathogenicity to common thrips and application thereof |
| CN114317294A (en) * | 2022-01-30 | 2022-04-12 | 华南农业大学 | Spaceflight entomogenous fungus strain SCAUHT56 with high pathogenicity and high ultraviolet resistance to common thrips and application thereof |
| CN114317291A (en) * | 2022-01-30 | 2022-04-12 | 华南农业大学 | Space breeding beauveria bassiana strain SCAUHT21 with high pathogenicity to common thrips and application thereof |
| CN114317293A (en) * | 2022-01-30 | 2022-04-12 | 华南农业大学 | Spaceflight entomogenous fungus strain SCAUHT18 with high pathogenicity and high ultraviolet resistance to common thrips and application thereof |
| CN114317292B (en) * | 2022-01-30 | 2023-05-12 | 华南农业大学 | Space breeding beauveria bassiana strain SCAUHT12 with high pathogenicity to common thrips and application thereof |
| CN114317291B (en) * | 2022-01-30 | 2023-05-12 | 华南农业大学 | Space breeding beauveria bassiana strain SCAUHT21 with high pathogenicity to common thrips and application thereof |
| CN114317293B (en) * | 2022-01-30 | 2024-03-26 | 华南农业大学 | High pathogenicity and high ultraviolet resistance space insect fungus strain SCAUHT18 for common thrips and application thereof |
| CN114317294B (en) * | 2022-01-30 | 2024-03-26 | 华南农业大学 | High pathogenicity and high ultraviolet resistance space insect fungus strain SCAUHT56 for common thrips and application thereof |
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