CN107418899B - Beauveria bassiana BbL25 strain for preventing and treating red imported fire ants and application thereof - Google Patents
Beauveria bassiana BbL25 strain for preventing and treating red imported fire ants and application thereof Download PDFInfo
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Abstract
The invention discloses a beauveria bassiana BbL25 strain for preventing and treating red imported fire ants. The strain is preserved in China center for type culture Collection in 2017, 2 months and 16 days, and the preservation number of the strain is CCTCC NO: and M2017045. The beauveria bassiana BbL25 strain is highly pathogenic to the red imported fire ants, and the semilethal concentration (LC) of the beauveria bassiana BbL25 strain to the red imported fire ants is 14 days after inoculation50) Is 8.15 multiplied by 105conidium/mL, and the strain has better field control effect on the red imported fire ants, 1 multiplied by 107The control effect of the spore suspension on the solenopsis invicta reaches 82.52 percent 14 days after the application, and is equivalent to the effect of a chemical insecticide, namely 0.25 percent of fluben bait. The beauveria bassiana BbL25 strain can be used as a living biological insecticide, has no pollution to the environment and no toxicity to people and livestock, is suitable for the production of organic agricultural products, and has a great application prospect.
Description
Technical Field
The invention belongs to the technical field of biological control. More particularly, relates to a beauveria bassiana BbL25 strain for preventing and treating red imported fire ants and application thereof.
Background
Solenopsis invicta Buren belongs to Hymenoptera Formicidae Myricidae Hymenoptera Solenopsis, and is a typical agricultural and forestry pest and causes serious damage to the invaded area. In China, red imported fire ants have invaded 11 provinces such as Taiwan, Guangdong, hong Kong, Macau, Fujian, Guangxi, Hunan, Hainan, Sichuan, Jiangxi, Yunnan and the like. The red fire ants have strong destructive power and aggressiveness, eat seeds of plants, prey on invertebrates, release venom through the thorns to bite human beings and wild animals, bite on insulating layers of electronic and electric equipment to destroy civil infrastructure and the like. Poses serious threats to human health, biodiversity, agriculture and forestry production and infrastructure safety.
The prevention and control of the solenopsis invicta mainly comprises methods such as physical prevention and control, chemical prevention and control, biological prevention and control and the like, wherein the method is mainly to apply chemical insecticide. However, the long-term use of chemical prevention can cause the drug resistance of the solenopsis invicta, and the use amount of chemical agents needs to be increased continuously in the prevention process, so that the prevention cost is increased continuously. In addition, excessive use of chemical pesticides is easy to cause harm and pollution to soil and non-target organisms around the soil, and environmental safety and biodiversity are influenced. The safety of chemical pesticide control has become a key factor limiting the control of solenopsis invicta, and the development and utilization of biological control methods to control solenopsis invicta has become a trend.
Recently, there have been a number of studies on the biological control of solenopsis invicta at home and abroad, including the control using natural enemy insects, predatory mites, parasitic nematodes and pathogenic microorganisms. The fungus pesticide has the advantages of wide host range, safety to environment, no drug resistance to pests and the like, and is an ideal means for preventing and controlling the solenopsis invicta. There are various entomogenous fungi that infect red imported fire ants, such as corynebacterium fumosoroseum Isaria, Metarhizium flavoviride, Metarhizium anisopliae and Beauveria bassiana, paecilomyces lilacinus, etc., and there have been some related patent publications: patent application No. 201610656107.0 discloses an uvioresistant metarhizium anisopliae mutant strain MaUV 1 (preservation number is CCTCC NO: M2016250) and application thereof, wherein the strain has strong infection insecticidal effect on drug-resistant diamond back moth, red imported fire ant and other pests, and strong uvioresistant capability; patent application No. 201510103305.X discloses a microbial pesticide composition for controlling red imported fire ants, wherein beauveria bassiana and thiacloprid are compounded to have a remarkable synergistic effect, so that the control effect is very good, and the composition has the advantages of high efficiency and high speed for the red imported fire ants; patent application No. 201310721119.3 discloses an isaria fumosorosea and avermectin oil suspending agent, a preparation method and application thereof, wherein the suspending agent is mainly used for controlling pests such as diamondback moth, red imported fire ant and the like; patent application No. 201120139109.5 discloses a device for inducing and collecting red fire ant infected entomogenous fungi, which has good effect of preventing and treating red fire ant, wherein conidia adhered to the surface of the plant oil in the device are conidia of P028 strain of Isaria javanicus; patent application No. 200810029158.6 discloses a paecilomyces javanicus strain and its application, the paecilomyces javanicus has strong infection insecticidal effect on pests such as whitefly, red imported fire ant, prodenia litura, aphid, diamond back moth, etc.; patent application No. 200810029745.5 discloses a paecilomyces javanicus and azadirachtin compound pesticide which has good control effect on pests such as red imported fire ants, prodenia litura, aphids, whiteflies, diamond back moths and the like.
In addition, some research reports about pathogenic entomogenous fungi of the solenopsis invicta are reported, and Lulihua et al (2011) find that the beauveria bassiana Bb02 and Bb04 strains have stronger pathogenicity and lethal speed by measuring the pathogenic capacity of 6 strains of entomopathogenic fungi from different sources to the solenopsis invicta, and the strains are excellent strains for the microbial control of the solenopsis invicta; liu Xiao Yan et al (2010) separate and identify pathogenic bacteria of red imported fire ants from Guangzhou Muyu district, Nansha district, Huizhou and Shenzhen city, and the determination finds that the isolate PL has certain pathogenic effect on the red fire ants; liu Xiao Yan et al (2014) set 17, 21, 25, 29 and 33 deg.C 5 temperature levels, and spray method is adopted to obtain concentration of 1 × 105And 1X 108Each mL-1The muscardine spore suspension liquid is respectively infected with the ergates of red fire ants, so as to test the pathogenicity of the muscardine strain to the ergates of red fire ants at different temperatures; 4 Beauveria bassiana strains sensitive to the solenopsis invicta are screened indoors (2009) and the results show that the 4 strains have certain potential in the biological control of the solenopsis invicta; wang Lei et al (2014) studied the toxicity of different doses of metarhizium anisopliae M09 to red fire ants, and the results showed that the mortality rate of red fire ants is in positive correlation with the dose of metarhizium anisopliae; a good biocontrol strain-green muscardine strain SM076 for preventing and controlling the harm of red imported fire ants is screened by the love, and the like (2013).
However, the effect of the pathogenic entomogenous fungi on controlling red fire ants is not ideal, the research is only carried out under indoor conditions, and because the indoor measurement conditions are different from the actual application, environmental factors such as temperature and humidity have great influence on the pathogenicity of white muscardine bureau (Libangchen and Leyochang, 1994; Dimpinella Paniculata, 2008), the control effect of the entomogenous fungi on the red fire ants in the field environment is not verified.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defects and shortcomings of pathogenic entomogenous fungi of solenopsis invicta in the prior art and provides a beauveria bassiana BbL25 strain for preventing and treating the solenopsis invicta. The beauveria bassiana BbL25 strain is highly pathogenic to red fire ants, has ideal field control effect, can be used as a living biological insecticide, has no pollution to the environment and no toxicity to people and livestock, is suitable for production of organic agricultural products, and has great application prospect.
The invention aims to provide a beauveria bassiana BbL25 strain for preventing and controlling red imported fire ants.
Another object of the present invention is to provide the use of the above Beauveria bassiana BbL25 strain.
The above object of the present invention is achieved by the following technical solutions:
a Beauveria bassiana (Beauveria bassiana) BbL25 strain for preventing and treating red imported fire ants, wherein the strain is preserved in China Center for Type Culture Collection (CCTCC) in 2017, 2 months and 16 days, and the preservation number of the strain is CCTCC NO: m2017045, the name number of the classification is Beauveria gossiana BbL 25; the preservation address is Wuhan university in Wuhan, China.
The strain is obtained by separating and purifying soil from Dutch showering of Balin Lufeng city of inner Mongolia, and is determined to be an entomogenous fungus strain with high efficiency to red fire ants through biological activity determination and biological characteristic research strain identification, and the classification status of the strain belongs to Deuteromycotina (Deuteromycina), Hyphomycetes (Hyphomycetes), Moniliales (Moniliales), Moniliaceae (Moniliaceae) and Beauveria (Beauveria).
The morphological characteristics of the strain on a potato glucose medium are as follows:
the colony is white in the initial stage and has a villous surface; the colony is circular when cultured on day 12, the center and the edge of the colony slightly bulge, most of the middle part of the colony is slightly sunken, the surface of the colony is white and villous, the back of the colony is light yellow, the diameter of the colony is (68.16 +/-1.96) mm, and the middle part of the later colony becomes powder. Under an optical microscope, hyphae have septa, branches and are transparent, and the width is (1.08 +/-0.14) mu m; the conidiogenous cells are recurrent or single, conidiophores are not branched, the tops of the conidiophores are expanded, and conidia grow densely on the conidiophores; conidiophore sphere or near sphere, unit cell, colorless, diameter (2.05 + -0.13) μm.
Further, the ITS1-5.8S-ITS4rDNA sequence of the beauveria bassiana BbL25 strain is shown in SEQ ID NO: 1 is shown.
Furthermore, the β -tubulin gene sequence of the beauveria bassiana BbL25 strain is shown in SEQ ID NO. 2.
The beauveria bassiana BbL25 strain of the invention has semilethal concentration (LC) to red fire ants on 14 days after indoor inoculation of the red fire ants50) Is 8.15 multiplied by 105conidium/mL and the strain has better field control effect on the solenopsis invicta, 1 multiplied by 107The control effect of the spore suspension on the solenopsis invicta reaches 82.52 percent 14 days after the application, and is equivalent to the effect of a chemical insecticide, namely 0.25 percent of fluben bait.
After the conidium suspension of the beauveria bassiana BbL25 strain is soaked in the red fire ants for 2-3 days, the red fire ants have the phenomena of slow action and cachexia and then die. Under an optical microscope, it can be observed that white hyphae firstly extend out of the body from the podosoma, the oral organ and the somite of the red imported fire ant cadaver, and a large amount of white conidia are generated on the hyphae with the passage of time.
Therefore, the application of the beauveria bassiana BbL25 strain in preventing and controlling red imported fire ants and the application in preparing the medicament for preventing and controlling the red imported fire ants are also within the protection scope of the invention.
Preferably, the method of application is: harvesting conidia of the beauveria bassiana BbL25 strain, and preparing the conidia into a 5 multiplied by 10 concentration by using 0.01-0.03% (preferably 0.02%) Tween 80 solution6～1×107Perml (preferably 1X 10)7mL) by spraying the spore suspension onto the solenopsis invicta for solenopsis invicta control. Particularly, the pesticide can be sprayed on the red imported fire ants when the red imported fire ants gather on the surfaces of ant nests so as to prevent and treat the red imported fire ants.
Meanwhile, the invention also provides a biological preparation for preventing and treating red imported fire ants, which comprises the beauveria bassiana BbL25 strain.
Preferably, the biological preparation comprises 5 × 106～1×107spores/mL of spore suspension of Beauveria bassiana BbL25 strain.
More preferably, the biological preparation comprises 1 × 107spores/mL of spore suspension of Beauveria bassiana BbL25 strain.
Meanwhile, the application of the biological agent in preventing and controlling the solenopsis invicta also belongs to the protection scope of the invention.
Compared with the prior art, the invention has the following beneficial effects:
(1) the beauveria bassiana BbL25 strain is high in efficiency on preventing and controlling red imported fire ants, and the semilethal concentration (LC) of the beauveria bassiana BbL25 strain on the red imported fire ants is 14 days after application50) Is 8.15 multiplied by 105/mL。
(2) The beauveria bassiana BbL25 strain has good field control effect on the red imported fire ants, and the field control effect is 1 multiplied by 107The control effect of the spore suspension on the solenopsis invicta reaches 82.52 percent 14 days after the application, and is equivalent to the effect of a chemical insecticide, namely 0.25 percent of fluben bait.
(3) The beauveria bassiana BbL25 strain is an entomopathogenic fungus, can be used as a biological living pesticide, has the characteristics of no pollution to the environment and no toxicity to people and livestock, is suitable for the production requirement of organic agricultural products, and has a wide application prospect.
Drawings
FIG. 1 shows the colony morphology of beauveria bassiana BbL25 strain when cultured on potato dextrose medium at 25 ℃ for 12 days; a is the front side and B is the back side.
FIG. 2 is a micrograph of spore-forming structures and conidia of Beauveria bassiana BbL25 strain; a is a spore-forming structure, and B is a conidium.
FIG. 3 is a morphological feature of Beauveria bassiana BbL25 strain when it infests red imported fire ants.
FIG. 4 shows specific amplification electrophoresis diagram of ITS1-5.8S-ITS4rDNA gene and β -tubulin gene of Beauveria bassiana BbL25 strain.
FIG. 5 shows a branched developmental tree of rDNA-ITS gene sequence of Beauveria bassiana BbL25 strain.
FIG. 6 shows a branched developmental tree of the β -tubulin gene sequence of Beauveria bassiana BbL25 strain.
Detailed Description
The invention is described in further detail below with reference to the drawings and specific examples, which are provided for illustration only and are not intended to limit the scope of the invention. The test methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are, unless otherwise specified, commercially available reagents and materials.
Example 1 isolation and identification of Beauveria bassiana BbL25 Strain
1. Strain isolation
The strain is obtained by separating and purifying soil of the Berlin dexisraella showering in Nemontage-Chifeng city.
2. Morphological characterization of strains
The obtained strain is cultured on a potato glucose culture medium, and the morphological characteristics of the strain are as follows: the colony is white in the initial stage and has a villous surface; the colony is circular by 12 days, the center and the edge of the colony are slightly raised, most of the middle part of the colony is slightly sunken, the surface of the colony is white and villous, the back surface of the colony is light yellow, the diameter of the colony is (68.16 +/-1.96) mm, and the middle part of the colony at the later stage is changed into powder (shown in figure 1). Under an optical microscope, hyphae have septa, branches and are transparent, and the width is (1.08 +/-0.14) mu m; the conidiogenous cells are recurrent or single, conidiophores are not branched, the tops of the conidiophores are expanded, and conidia grow densely on the conidiophores; conidiophores are spherical or nearly spherical, monosporous, colorless, and have diameters (2.05. + -. 0.13) μm (as shown in FIG. 2).
3. Molecular characterization of strains
The electrophoresis pattern of ITS1-5.8S-ITS4rDNA gene specific amplification product and β -tubulin gene specific amplification product of the strain is shown in figure 4, and the corresponding ITS1-5.8S-ITS4rDNA gene sequence and β -tubulin gene sequence are sequentially shown in SEQ ID NO: 1-2.
The ITS1-5.8S-ITS4rDNA gene sequence was aligned in the NCBI database, and the alignment results showed that the strain sequence was highly similar to the sequences of multiple Beauveria bassiana (Beauveria bassiana) strains. Constructing phylogenetic clade of the strain, a plurality of beauveria bassiana strains and Isaria fumosorosea strains serving as outer groups; as shown in FIG. 5, the isolated strains of the present invention were clustered on the same branch with all Beauveria bassiana, and the genetic relationship was highly homologous.
Comparing β -tubulin gene sequence in NCBI database, the result shows that the strain sequence is highly similar to the sequences of multiple Beauveria bassiana (Beauveria bassiana) strains and a Beauveria bassiana (Beauveria brongniartii) strain, the strain is constructed with Beauveria bassiana, Beauveria bassiana, some similar strains and with corynebacterium fumosoroseum (I.fumosorosea) as outer group, the result is shown in figure 6, the separated strain is highly homologous with Beauveria bassiana and a Beauveria bassiana on the same branch.
Thus, the strain was identified as Beauveria bassiana (Beauveria bassiana) by combining the ITS1-5.8S-ITS4rDNA sequence claded developmental tree and the β -tubulin gene sequence claded developmental tree, and the strains classified as Beauveria bassiana (Deuteromycetina), Hyphomycetes (Hyphomycetes), Moniliales (Moniliales), Moniliaceae (Moniliaceae), Beauveria (Beauveria).
The strain is named as Beauveria bassiana (Beauveria bassiana) BbL25 strain and is preserved in China Center for Type Culture Collection (CCTCC) in 2017, 2 months and 16 days, and the preservation number of the strain is CCTCC NO: m2017045, the name of the classification name is Beauveria gossiana; the preservation address is Wuhan university in Wuhan, China.
Example 2 determination of virulence of Beauveria bassiana BbL25 strain against Solenopsis invicta
1. Spore suspension preparation
(1) Conidium production
A slant strain of the beauveria bassiana BbL25 strain stored in the example 1 is taken, a small amount of conidia are scraped and inoculated on a PDA plate, the strain is cultured in an incubator at 25 ℃ until a bacterial colony is nearly full of a culture dish, and the conidia on the surface layer of the bacterial colony are scraped for standby.
(2) Molecular spore suspension preparation
Placing the conidia scraped in the step (1) into a centrifuge tube, adding 0.02% Tween-80 solution, shaking uniformly, measuring the spore concentration by using a blood counting chamber, and preparing conidia suspensions with different required concentrations for later use.
Conidia suspensions of beauveria bassiana BbL25 strain were prepared at the following concentrations:
(1)1×106per mL;
(2)5×105per mL;
(3)1×105per mL;
(4)5×104per mL;
soaking in the above 4 spore suspensions with different concentrations under laboratory conditions, continuously investigating the death rate of Solenopsis invicta for 14 days, and analyzing and calculating the lethality and lethal medium concentration (LC) of each concentration by SPSS software50) (ii) a The calculation formula is as follows:
mortality (%). mortality vs. number of dead insects/number of insects before treatment X100%
Corrected mortality (%) - (treatment mortality-control mortality)/(1-control mortality) × 100%
2. Results
The results show that 14 days after the impregnation treatment, conidia of the beauveria bassiana BbL25 strain have the toxicity effect on the red imported fire ants: 1X 1065X 10 units/mL51X 10 units/mL55X 10 units/mL4The 4 concentrations per mL corresponded to corrected mortality rates of 64.77%, 38.10%, 9.52%, 19.05%, respectively (as shown in table 1). Performing regression analysis on conidium concentration and red imported fire ant corrected mortality by SPSS 17.0 software, and performing semi-Lethal Concentration (LC) of treated beauveria bassiana50) Is 8.15 multiplied by 105conidia/mL (shown in Table 2), the results show that the Beauveria bassiana BbL25 strain has good treatment effect on the Solenopsis invicta.
TABLE 1 virulence test results of BbL25 strain spore suspensions on Solenopsis invicta at different concentrations
| Concentration/ |
1×106 | 5×105 | 1×105 | 5×104 |
| Correcting mortality | 64.77％ | 38.10％ | 9.52％ | 19.05％ |
TABLE 2 BbL25 model of virulence probit of Solenopsis invicta and its semilethal concentration (LC)50)
| Equation of model | Chi fang check pb | LC50And 95% confidence interval (spore/mL) |
| Y＝1.233X-7.286a | 0.695 | 8.15×105(3.04×105-2.31×106) |
a: the concentration X is converted using a base 10 logarithm
b: in the result of goodness-of-fit test, p of pearson chi-square test is greater than 0.05, which indicates that no problem exists in the degree of fitting
This example also observed the infestation of the red imported fire ants by beauveria bassiana BbL25 strain in conjunction with the bioassay. Observing the morphological characteristics of the red imported fire ants infected by the red imported fire ants under a dissecting mirror on 2-4 days after the death of the red imported fire ants respectively, wherein the results are shown in a figure 3: after the Beauveria bassiana conidia suspension is soaked in the red fire ants, the red fire ants have the phenomena of slow action and cachexia 2-4 days, and then die. Under an optical microscope, it can be observed that white hyphae firstly extend out of the body from the podosoma, the oral organ and the somite of the red imported fire ant cadaver, and a large amount of white conidia are generated on the hyphae with the passage of time.
EXAMPLE 3 determination of the pathogenicity of several entomogenous fungi on Solenopsis invicta
1. The method for producing conidia according to the step (1) of the above example 2 was carried out by formulating MS-01, MS-02, MS-03, MS-05, MS-06, MS-08, MS-13, MS-22, L18 and BbL25 strains and Java strain isolated from Spodoptera litura and Corynebacterium fumosoroseus (IfB01) isolated from Bemisia tabaci in Guangzhou into 1.0X 10 strains respectively in soils from different sites of inner Mongolia7spores/mL, in laboratory conditions, red imported fire ant were treated by impregnation with a spore suspension, and the mortality rate of red imported fire ant was investigated for 14 consecutive days after treatment.
Mortality (%). mortality vs. number of dead insects/number of insects before treatment X100%
Corrected mortality (%) - (treatment mortality-control mortality)/(1-control mortality) × 100%
2. The corrected mortality for bemisia tabaci for the different strains is shown in table 3; the results showed that beauveria bassiana BbL25 strain had the highest corrected mortality rate for solenopsis invicta, 87.29%, showing strong pathogenicity for solenopsis invicta.
TABLE 3 determination of the pathogenicity of the different entomogenous fungi strains on Solenopsis invicta (1X 10)7mL)
Example 4 field efficacy test of Beauveria bassiana BbL25 strain on Solenopsis invicta
1. According to the method for producing conidia according to the step (1) in the above example 2, conidia of beauveria bassiana were added to a 0.02% tween-80 solution, and the experimental group and the control group were set at the following concentrations or methods:
(1)1×106a spore suspension/mL;
(2)5×106a spore suspension/mL;
(3)1×107a spore suspension/mL;
(4) positive control: 0.25% of fluben bait (trade name: ant King, Yongdong Zhaoqing city, ancient lake region, Bio-preparation Limited);
(5) blank control: and (4) clear water.
The experiment is carried out in Ningxi teaching and scientific research base of south China agricultural university, and 3 treatments (1 multiplied by 10) are set6，5×106And 1X 107spores/mL suspension), 4 nests per treatment were replicated 3 times, with 0.25% fluben bait compared to clear water. When the pesticide is applied, the ant nest is stirred by a thin wood rod to attract a large number of red fire ants to climb out of the surface of the ant nest, conidium suspensions of the BbL25 beauveria bassiana strains with different concentrations are respectively sprayed until the ant nest is completely wetted, and the ant nest is marked after treatment. Before application, and 7 days and 14 days after application, trapping Solenopsis invicta in trapping bottle with built-in ham sausage for 30min near the ant nest, counting, and calculating the insect population reduction rate and control effect of Solenopsis invicta.
Rate of insect population decline (%) (1-total number of solenopsis invicta lured by post-drug treatment luring bottle/total number of solenopsis invicta lured by pre-drug luring bottle) × 100
Control effect (%) < 100 × (treatment decline rate-CK decline rate)/(100-CK decline rate)
2. The test result shows that the beauveria bassiana BbL25 strain has better control effect on the solenopsis invicta in the wild, and the control effect of the strain is correspondingly increased along with the increase of the concentration of the spore suspension. When 1X 107The control effect of the/mL spore reaches 67.14% and 82.52% within 7 days and 14 days after application, respectively, and is equivalent to the effect of 0.25% of the sulfluramid bait (Table 4).
TABLE 4 field control of Solenopsis invicta with Beauveria bassiana BbL25 strain
Note: letters after the number indicate significant differences after analysis by the new repolarization method (DMRT), and differences in letters indicate significant differences (p < 0.05).
The beauveria bassiana is subjected to a field pesticide effect test by using a spraying method, and compared with a chemical agent for preventing and treating red imported fire ants, the beauveria bassiana is relatively high in prevention and treatment effect and long in persistence.
SEQUENCE LISTING
<110> southern China university of agriculture
<120> beauveria bassiana BbL25 strain for preventing and treating red imported fire ants and application thereof
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gagttcaaca tgaagaatct cgtcaa 506
Claims (7)
1. The beauveria bassiana BbL25 strain for preventing and treating red imported fire ants is characterized in that the strain is preserved in China Center for Type Culture Collection (CCTCC) in 2017, 2 months and 16 days, and the preservation number of the strain is CCTCC NO: and M2017045.
2. Use of the beauveria bassiana BbL25 strain of claim 1 for controlling solenopsis invicta.
3. The application according to claim 2, wherein the method of application is: harvesting conidia of the beauveria bassiana BbL25 strain, and preparing the conidia into a 5 multiplied by 10 concentration solution by using 0.01-0.03% Tween 806～1×107The spore suspension is sprayed on the red imported fire ant by a spraying method to control the red imported fire ant.
4. Use of the beauveria bassiana BbL25 strain of claim 1 in the preparation of a medicament for the control of red imported fire ants.
5. A biological agent for controlling solenopsis invicta, comprising the beauveria bassiana BbL25 strain of claim 1.
6. The biological agent according to claim 5, wherein the biological agent comprises 5 x 106～1×107Spore/ml spore suspension of beauveria bassiana BbL25 strain.
7. Use of the biological agent according to claim 5 or 6 for controlling solenopsis invicta.
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| CN110129209B (en) * | 2019-05-14 | 2022-05-20 | 西南林业大学 | Violet purpurea fungus strain and application thereof |
| CN113150999A (en) * | 2020-12-17 | 2021-07-23 | 华南农业大学 | Entomogenous fungus strain with high pathogenicity to prodenia litura and application thereof |
| CN113684136B (en) * | 2021-07-21 | 2023-08-22 | 华南农业大学 | Broad-spectrum insecticidal beauveria bassiana strain and application thereof |
| CN114480138B (en) * | 2022-01-11 | 2023-08-18 | 长江师范学院 | Beauveria bassiana LZ2-1a and application thereof |
| CN115927006A (en) * | 2022-10-24 | 2023-04-07 | 华南农业大学 | Beauveria bassiana and application thereof in preventing and controlling red imported fire ants |
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| CN103981108A (en) * | 2014-06-04 | 2014-08-13 | 河北省农林科学院植物保护研究所 | Beauveria bassiana for preventing radysia odoriphaga and application thereof |
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| CN104686500B (en) * | 2015-03-09 | 2017-03-29 | 广东省生物资源应用研究所 | Injecting type biology preventing control method in a kind of flourishing ant nest |
| CN105039184B (en) * | 2015-09-07 | 2018-09-21 | 福建省林业科学研究院 | A kind of muscardine bacterial strain and its application to the miscellaneous caterpillar larva of ripple with pathogenicity |
| CN106857683A (en) * | 2017-03-30 | 2017-06-20 | 福建省龙梦农生物科技有限公司 | A kind of biological source bait formulation for preventing and treating red fire ant |
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| CN103981108A (en) * | 2014-06-04 | 2014-08-13 | 河北省农林科学院植物保护研究所 | Beauveria bassiana for preventing radysia odoriphaga and application thereof |
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