CN114317293A - Spaceflight entomogenous fungus strain SCAUHT18 with high pathogenicity and high ultraviolet resistance to common thrips and application thereof - Google Patents
Spaceflight entomogenous fungus strain SCAUHT18 with high pathogenicity and high ultraviolet resistance to common thrips and application thereof Download PDFInfo
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Abstract
The invention relates to the field of biological control, in particular to an excellent space strain with high pathogenicity and high ultraviolet resistance on common thrips, which is beauveria bassiana (Beauveria bassiana)Beauveria bassiana) The strain SCAUHT18 is preserved in Guangdong province microorganism culture Collection (GDMCC), and the preservation number is GDMCC NO: 62188. The invention comprehensively considers the toxicity and the ultraviolet resistance of the original strain and the space mutation strain to the common thrips by combining the indexes of strain screening, reasonable experimental schemes and the like. The screened bacterial strains with excellent pathogenicity and high ultraviolet resistance to the common thrips have great potential for biological control of the common thrips.
Description
Technical Field
The invention relates to the field of plant protection, in particular to the field of biological control, and especially relates to biological control of common thrips.
Background
Along with the sustainable development, the protection of the ecological environment is of great importance. In the development process of agricultural technology, unreasonable application of pesticides can easily cause various ecological environment pollution problems. In the production, chemical control is usually adopted, which not only has unsatisfactory effect but also causes a series of problems of drug resistance of pests, mass death of natural enemy insects, loss of biological diversity, overproof pesticide residue, ecological environment pollution and the like (Yangyuangjun. harmfulness of pesticide and green plant protection technology discussion [ J ] agricultural development and equipment, 2021(04): 132-. With the continuous development of agricultural economy in recent years, the usage amount of pesticides is continuously increased, the pesticide amount used for preventing and treating crop diseases and insect pests in the world is about 600 million t every year, the effective utilization rate of the pesticides is only 30%, and the rest 70% of the pesticide amount belongs to the loss amount (Heyufeng. research on harmfulness of pesticides and green plant protection technology [ J ]. agricultural technology and equipment, 2021(01): 94-95.). Pesticide residues in the environment can be continuously diffused through weather conditions such as rain, wind and the like, so that a large amount of pesticide residues are formed in global air, oceans, soil, organisms and the like. China belongs to a big agricultural country, and the agricultural production yield is quite large, so that the pesticide dosage in China is promoted to occupy the top of the world. The problem of pesticide pollution is getting worse and even the case of pesticide poisoning frequently occurs because of the continuous expansion of the pesticide usage amount (Liuguan. pesticide environmental hazard problem and countermeasure research [ J ] Green technology, 2020(12): 158-.
At the moment, a consciously reinforced green plant protection technical mode is needed, and the prevention and control work of the pesticide pollution problem is reduced as much as possible. Therefore, the method changes the traditional control mode mainly using chemical pesticides, carries out the plant protection policy of 'prevention is mainly and comprehensive control', adheres to the basis of agricultural control and biological control, and has extremely important significance in sustainable health-building development for comprehensively controlling pests by combining other control measures (Yangyuan Hua, Dukai Boo, Shimingwang. biological control research progress of entomogenous fungi [ J ]. proceedings of Henan academy of science and technology, 2011, 39(1): 34-37).
Entomogenous fungi are a class of parasitic fungi that enter the body of an insect by infestation and absorb insect nutrition ultimately leading to death of the host. It has different action mode from chemical pesticide, is safe to environment and human body, and is commonly used for biological control of pests. There are two main ways in which entomogenous fungi infect insects: external routes, contact with insects, invasion from body walls, spiracles and wounds, etc.; the internal pathway, when the insect takes food and breathes, invades through the digestive tract and respiratory tract. External infection is exemplified by body surface invasion, firstly entomogenous fungi is attached to the body surface of a host as conidia, then the conidia germinate to grow a germ tube, the germ tube penetrates through the host body wall to enter the blood cavity of the host, and internally and rapidly propagates in the form of mycelium or through small independent propagules, so that nutrients of the entomogenous fungi are absorbed in the blood lymph and toxic substances are continuously generated and released, and finally the death of the host insects is caused (plum, ginger spring jee, Zhaoyying, etc.. application of entomogenous fungi in forestry pest biological control [ J]Plant protection, 2016, 10(20): 10-24). The internal invasion is the invasion of entomogenous fungi through the alimentary canal, respiratory tract and other routes of insects, and then the entomogenous fungi germinate and grow in a host body until the nutrition in the insect body is completely consumed. At present, more than 400 entomogenous fungi species are reported in China, wherein metarhizium anisopliae isMetarhiziumspp. and Beauveria bassianaBeauveriaspp is the most widely used for commercial promotion and application. Beauveria bassiana (Vuill.) VuillBeauveriaThe host range is wide, including important agricultural and forest pests, vector insects and acarids, the insect pest killer is easy to culture, is harmless to warm-blooded animals and plants, has strong disease control capability on adults, can infect larvae, pupae, adults and other insect states, has a continuous effect on the next generation, and plays an important role in the management of agricultural and forest pests. The entomogenous fungi are well known for the advantages of various species, safety, effectiveness, long application period, no harm to natural enemies, difficult generation of resistance, rapid mass production and the like, and are applied to organismsThe application research of entomogenous fungi in biological pest control [ J]Shandong science, 2005, 18(4): 37-40.).
Space breeding is a high-tech breeding approach and method for breeding new plant germplasm and new material on the ground and cultivating new species by utilizing the beneficial variation generated by the space environment which can be reached by recoverable satellites and the like on the mutagenesis of plants (seeds), and is called space breeding (Macheng, Mawei super, Anjian Hei, plum man. China. application and research progress of microbial space mutagenesis breeding [ J]Hunan agriculture university, 2012(19): 5-8.). The specific conditions of the space environment may cause genetic variation of organisms, and the specific conditions comprise ultrahigh vacuum, ultra-cleanness, microgravity, strong radiation, great difference from the ground conditions, strong ultraviolet irradiation and the like. Space environment is a particularly important area of space science research. High mutation frequency, wide mutation spectrum, large mutation amplitude and stable mutation character after mutation, thereby shortening breeding cycle and improving biological safety (Lionhead G, Vanderbrink J P, hoeksmem J D, et al. The impact of mutated microbiology on The growth of differential genes of The modulated plant medical great project [ J]. Microgravity Science and Technology, 2018, 30(4): 491-502.). The space mutation of the microorganism provides a new way for the mutation breeding of the microorganism, and the space mutation can obviously change the growth character of the microorganism in the aspects of growth speed, growth form, growth characteristic and the like. Space induction is changed into genetic improvement of a biological control excellent strain, a new way is provided, and entomogenous fungi carried by space possibly improve the toxicity of the strain and strengthen the stress resistance of the strain to the environment under the action of space environment, so that high-efficiency engineering bacteria are obtained. These changes in the trait result in a change in the ability of the microorganism to produce metabolites. Compared with the traditional mutation breeding under the same condition, the space mutation breeding has obvious effect, better genetic stability of characters, great application potential and great channel brought byEconomic benefit (Jiangbena, Zhang Pinna, Wang haisheng, Xiao traceu, Yang cheng, New Cone. Shi Bacterin engineering bacteria space mutagenesis effect and screening of high-yield strains thereof [ J]Chemical journal, 2010,61(02): 455-461).
Common Japanese thistleMegalurothripsusitatus(Baball), also known as Strychnos faberi and Strychnos japonicum, is of the order ThysanopteraThysanopteraThrips subfamilyThripina（Stephens）PriesnerCircium genusMegalurothripsInsects widely distributed in the world wide tropical regions of China, Malaysia, India, Australia, etc. (economic insects of Han Korea, Israel, Thysanoptera M]Beijing: scientific Press, 1997: 39-59.). At present, the prevention and treatment of thrips also mainly depends on chemical prevention and treatment means. The insect genus omnivorous pest has 28 kinds of 9 families according to incomplete statistics, wherein 16 kinds of the host plants are leguminous plants (Miyazaki M, Kudo I, Iqbal A. Notes on the thrips (Thysanoptera) curing on the soybean in Java [ J ] M]. library wur, 1984, 52(4): 482-486.). Can be harmful during the whole growth period of the host plant, and the harm is mainly to file and suck the juice of young tissues and organs such as growing points, floral organs and the like of the host plant, so that the leaves are shrunk, become small, bent or deformed, and when the plant grows slowly or stops, the plant prefers to eat floral organs and fruits. However, due to the small size, good hiding, strong reproduction ability, wide adaptability to life habits of thrips, etc., the drug resistance of thrips has been increasingly enhanced in recent years, and it is difficult to achieve good control effect of chemical control means (research on the types of thrips in the Tang-national culture, Gong-letter culture, Meng-Gung-Wuhan region [ J ] on the types of thrips in Wuhan region]The university of agriculture, Huazhong, 2002, 21(1): 5-9.). Due to the long-term use of chemical pesticides, some pests have strong drug resistance, and many natural enemies of the pests are mistakenly killed in the control process. The problems of ecological balance destruction, environmental pollution and food safety of farmlands caused by chemical control are solved, and in recent years, common thrips has different degrees of drug resistance to various common insecticides.
Entomogenous fungi are affected by various environmental factors in each stage of life history, such as ultraviolet irradiation, temperature, humidity and stress of other biological or non-biological factors, the factors can interact to influence the activity and the infectivity of spores, the large-scale application of fungus pesticides in production is also restricted, and the screening of strains with high toxicity and high stress resistance is very important for field production and popularization.
Disclosure of Invention
The invention screens out the strain with higher ultraviolet resistance from the high-toxicity beauveria bassiana strain induced in the voyage, and applies the strain to the biological control technical means of the common thrips. Therefore, the invention aims to compare the original strain and induce the uvioresistant performance of a high-toxicity strain in space flight and research and develop the high-toxicity strain, and provides a space return beauveria bassiana strain which has high toxicity and high uvioresistant performance on common circium japonicum. The strain has excellent insecticidal effect on common thrips and has the advantage of high ultraviolet resistance. In order to obtain an excellent strain with biological control effect on common thrips, the original strain and the early aerospace return are determined to be beauveria bassiana strains with high toxicity on the common thrips, the inventor adopts the research on the biological characteristics of the aerospace high toxicity strain to determine the survival rate of conidia under the irradiation of different ultraviolet time, and thus, the excellent strain with strong pathogenicity on the common thrips female and high ultraviolet resistance is obtained. The sporulation quantity, the colony growth rate, the spore viability and the like of the strain under the ultraviolet irradiation at different time are important indexes for screening excellent strains.
Experiments show that 20 strains of aerospace strains with high toxicity to common thistle and the original strain may have different ultraviolet resistances, so that the aerospace strains have different ultraviolet resistances. According to the experimental result, the ultraviolet resistance is considered comprehensively by combining the indexes of excellent strain screening, wherein the ultraviolet resistance is an important index for measuring the outdoor biological control potential of the high-toxicity fungus strain, the colony diameter, the spore yield and the spore germination rate of the aerospace strains SCAUHT18, SCAUHT38 and SCAUHT56 are higher than those of other aerospace strains and original strains under the ultraviolet irradiation of 0h, 1h, 2h, 4h and 8h, and the ultraviolet resistance of the aerospace strains SCAUHT18, SCAUHT38 and SCAUHT56 is more stable than those of the original strains and other test strains.
Furthermore, the invention also provides the aerospace strain SCAUHT18 on common thripsThe strain is preserved in Guangdong province microorganism culture collection center (GDMCC, microbial research institute of Guangdong province institute of science institute of 5-floor of Guangdong province No. 59 great institute of Mieli Zhou Lu 100, Guangzhou, China) at 2022, 1 month and 4 days, the preservation number is GDMCC NO:62188, and the classified name is GDMCC NO:62188Beauveriabassiana. Whereas the aerospace strains SCAUHT38 and SCAUHT56 will be patented by additional.
In specific implementation, the strain can be cultured to prepare spore liquid or a microbial inoculum containing spores. Preferably, the spore concentration of the strain at the time of administration is 106 spore/mL to 109spores/mL, most preferably 107 spore/mL to 108spores/mL.
The inventor discovers the original strain beauveria bassiana strain HNSB110 which has a certain effect on common thrips in the past in screening of the original strain and the aerospace strain, and the beauveria bassiana strain HNSB110 has a particularly prominent effect on the common thrips after return of aerospace mutagenesis and obviously improves the biological characteristics. Biological experiments show that the control effect of the space mutation strain on the common thrips reaches 90 percent (10 percent)7 spore/mL) whereas the effect of the original strain is only 60% under the same conditions; at 1X 107 Under the condition of spore/ml concentration solution, the colony diameter, spore yield and spore germination rate of the SCAUHT18, SCAUHT38 and SCAUHT56 are all obviously higher than those of other strains under ultraviolet irradiation of different treatment time, and when the ultraviolet irradiation is carried out for 8 hours, the colony diameter of the 10 th day reaches: 40mm, 40mm and 37.5mm, and the spore yield reaches: 2.6X 107/mL、2.35×107/mL、2.4×107Per mL; the germination rate reaches 72 h: 62.5%, 60.5%. Much higher than other strains (see experimental results and tables for specific examples). Therefore, the beauveria bassiana strains SCAUHT18, SCAUHT38 and SCAUHT56 can effectively inhibit outdoor common circium japonicum populations, are safe to people, livestock, plants and environment, can partially replace thrips chemical prevention pesticides, and have great application potential in outdoor biological prevention and control.
Drawings
FIG. 1 is a schematic diagram of the aerial breeding steps of the present invention.
FIG. 24 PE tubes of mutagenized strains obtained by space breeding.
FIG. 3 comparison of the colony diameters of strains SCAUHT18, SCAUHT38, SCAUHT56 and the wild type strains. Wherein A is a front view of the flat panel, and B is a back view of the flat panel.
Detailed Description
The following description is given by way of development and specific embodiments of the present invention, and is not intended to limit the invention thereto.
The first embodiment is as follows: space breeding
1. Test strains
HNSB 110: beauveria bassiana (balsamo) Vuillemin: (B)Beauveriabassiana) The strain is separated from forest soil in Kunming city of Yunnan province in 9 months in 2007, and is deposited in biological control center of biological control education department of southern China agricultural university.
As early experiments show that the strain has a high growth speed and a certain control effect on common thrips, the strain is selected for space breeding.
2. Experimental methods
The original strain HNSB110 is inoculated into 4 PE tubes, the PE tubes are sealed by preservative films, and the samples are collected and then put into bins in an experimental box. The airship is launched in space for on-orbit test, the returning capsule is retracted, the returning capsule is opened, and after the test box is opened, the sample is finally distributed (the specific operation steps are shown in figure 1).
3. Conditions of the experiment
(1) Carrier: long-symbol five-number B carrier rocket
(2) Time: 2020.5.5-2020.5.8 (67 hours of on-orbit flight)
(3) Launching site: hainan Wenchang space station
(4) Height on track: 300 to 8000 km
(5) The environment is as follows: multiple crossing Van Allen radiation band (high energy particle radiation band)
4. Results of the experiment
4 PE tubes (shown in figure 2) of mutant strains are obtained in total, and the strains are carried and returned, stored in the biological control center of the department of biological control education of southern China university and stored at 4 ℃ for subsequent experiments.
Example two: bioassay of strains
1. Test insects: the common thrips species is collected from Chongcun cowpea field in Guangzhou, and is raised in laboratory for several generations under the conditions of 26 deg.C, 65% relative humidity and L: D = 12: 12 photoperiod.
2. Test strains:
(1) HNSB 110: beauveria bassiana (balsamo) Vuillemin: (B)Beauveriabassiana) Is preserved in the biological control center of the department of biological control education of southern China university of agriculture and is an original wild strain.
(2) 100 single colony spores were picked up using the aviation mutant strains obtained in example one to obtain 100 space-borne strains. The primary toxicity test of common thrips was performed on 100 space strains, and then the bioassay test of common thrips was performed on the first 20 strains with higher toxicity.
3. Experimental methods
(1) Preparation of spore suspension
Culturing at 26 + -1 deg.C on PDA plate for 7 days, eluting conidia of entomogenous fungi with 0.05% Tween-80 sterile water, stirring with magnetic stirrer, shaking at 180 rpm in shaking table at 25 deg.C for 25 min, filtering with double-layer mirror paper, counting with blood counting plate, measuring mother liquor concentration, and making into 1 × 107 spores/mL of spore suspension.
(2) Determination of toxicity of entomogenous fungi to common thrips
Placing the prepared spore suspension and fermentation liquor in a flat-bottom finger-shaped tube (15 mm multiplied by 75 mm), soaking for 2h, pouring out the spore suspension, and naturally drying the finger-shaped tube for later use; cutting cowpea into segments (1 cm, without holes at both ends), soaking in spore suspension, taking out for 30s, air drying, placing into treated finger-shaped tube, simultaneously inoculating 50 heads of female common thrips, sealing cotton, placing in artificial climate box, and repeating each treatment for 4 times with 0.05% Tween-80 sterile water as blank control. Mortality was recorded for 7 consecutive days.
(3) Data processing
SPSS 19.0 software is used for experimental treatment analysis, single-factor analysis of variance is used for analyzing each result, and Tukey is used for detecting the significance of difference.
4 results of the experiment
The experimental results show that the pathogenicity difference of different strain spore suspensions on common thrips is obvious, the pathogenicity difference of different strains at the same concentration and different treatment time exists, and the death rate of the common thrips female imagoes is increased along with the increase of the concentration and the treatment time (Table 1); when the concentration is 1X 107At spore/mL (Table 1), the cumulative mortality rates at 6d for SCAUHT18, SCAUHT38, SCAUHT56 were 100%, 100%
The results show that the strains SCAUHT18, SCAUHT38 and SCAUHT56 have better lethal effect on common thrips, and belong to potential excellent biocontrol bacteria.
TABLE 1 corrected mortality of different strains of common thrips (%)
Note that different lower case letters in the same column indicate significant pathogenicity difference between different strains (P < 0.05) by Tukey test.
Example three: anti-ultraviolet biological characteristics of aerospace high-toxicity strain
1. Test strains
HNSB 110: beauveria bassiana (balsamo) Vuillemin: (B)Beauveriabassiana) Is collected at the center of biological control department of biological control education of southern China university of agriculture.
20 strains selected in the second strain of example. Wherein SCAUHT18, SCAUHT38 and SCAUHT56 are preserved in Guangdong province microorganism culture Collection (GDMCC), and the preservation numbers are GDMCC NO:62188, GDMCC NO: 62187 and GDMCC NO: 62199 respectively.
2. Test method
(1) Preparation of spore suspension
Culturing at 26 + -1 deg.C on PDA plate for 7 days, eluting conidia of entomogenous fungi with 0.05% Tween-80 sterile water, stirring with magnetic stirrer, shaking at 180 rpm in shaking table at 25 deg.C for 25 min, filtering with double-layer mirror paper, counting with blood count plate, measuring mother liquor concentration, and concoctingMaking into 1 × 107spores/mL of spore suspension.
(2) Ultraviolet irradiation
Will be 1 × 107spores/mL of spore suspension were irradiated in ultraviolet (UV-B) for 0, 1, 2, 4, and 8h, respectively.
(3) Colony diameter, spore yield and germination rate
200 mu L of suspension is dripped into the center of a PDA plate, the PDA plate is cultured for 10 days at 26 ℃, the colony diameter is measured and recorded every day, after the measurement of the last day is completed, 100 mL of 0.05 percent Tween-80 is used for elution to prepare conidium suspension, and the conidium yield is calculated by using a blood counting chamber.
Adding 2mL of the suspension into 18mL of SDA medium, shaking gently and mixing uniformly, placing the mixture in a shaking table at the temperature of 25 ℃ and rotating at 160 rpm, and performing microscopic examination on the germination condition of each treated spore for 24h, 48h and 72h respectively.
(4) Data processing
Performing experimental treatment analysis by using SPSS 19.0 software, analyzing each result by adopting one-factor analysis of variance, and testing the significance of difference by using Tukey
3. Results of the experiment
(1) Colony diameter and spore yield
The experimental results show that after 0, 1, 2, 4 and 8 hours of (UV-B) irradiation, the colony diameters and the spore yields of the aerospace strains SCAUHT18, SCAUHT38 and SCAUHT56 are obviously higher than those of the original strains and other strains after 10 days of growth compared with the colony diameters of the original strains, the colony diameters and the spore yields of the aerospace strains SCAUHT18, SCAUHT38 and SCAUHT 56. After 4h of (UV-B) irradiation, the colony diameters of the aerospace strains SCAUHT18, SCAUHT38, SCAUHT56 at 10d were 49.5mm, 45mm, 47mm, while the colony diameter of the original strain HNSB110 was: 31.5 mm. The original strain HNSB110 sporulation amount is 1.5 × 10 at 10 days7And the sporulation yield of the aerospace strains SCAUHT18, SCAUHT38 and SCAUHT56 at the 10d are respectively as follows: 3.3X 107/mL、3.35×107/mL、2.95×107/mL。
The results show that: under the irradiation of different ultraviolet times, the colony diameters (shown in figure 3, wherein A is a front view of a flat plate, and B is a back view of the flat plate) and the spore yields of space strain space strains SCAUHT18, SCAUHT38 and SCAUHT56 are both larger than those of original strains and other space strains, so that the space strain space strains have better spore activities, and belong to potential field excellent biocontrol bacteria.
TABLE 2 colony diameters of different strains under UV irradiation at different times
Note that different lower case letters in the same column indicate significant differences in colony diameter between strains (P < 0.05) by Tukey's test.
TABLE 3. different strains exposed to UV radiation at different times at 1X 106Spore yield/mL
Note that different lower case letters in the same column indicate significant difference in spore yield between different strains (P < 0.05) by Tukey's test.
(2) Germination rate
The experimental results show that after different ultraviolet irradiation times, the spore germination rates of the aerospace strains SCAUHT18, SCAUHT38 and SCAUHT56 at 24h, 48h and 72h are higher than those of the original strain and other strains. After 4h of ultraviolet irradiation, the germination rates of the aerospace strains SCAUHT18, SCAUHT38 and SCAUHT56 at 24 are 26.5%, 24.5% and 24%, and the germination rate at 48h is as follows: 38.5%, 36.5%, germination rate at 72 h: 74.5%, 72.5%, 70.5%. And after the original strain is subjected to ultraviolet irradiation for 4 hours, the germination rates of the original strain at 24 hours, 48 hours and 72 hours are as follows: 11.5%, 28.5% and 52%.
The results show that the aerospace strains SCAUHT18, SCAUHT38 and SCAUHT56 have higher spore germination rates than the original strains and other aerospace strains, and belong to potential excellent field biocontrol bacteria.
TABLE 4 germination rates of different strains under UV 0h irradiation
Note that different lower case letters in the same column indicate significant differences in germination rates between different strains (P < 0.05) by Tukey's test.
TABLE 5 germination rates of different strains under UV 1h irradiation
Note that different lower case letters in the same column indicate significant differences in germination rates between different strains (P < 0.05) by Tukey's test.
TABLE 6 germination rates of different strains under UV 2h irradiation
Note that different lower case letters in the same column indicate significant differences in germination rates between different strains (P < 0.05) by Tukey's test.
TABLE 7 germination rates of different strains under UV 4h irradiation
Note that different lower case letters in the same column indicate significant differences in germination rates between different strains (P < 0.05) by Tukey's test.
TABLE 8 germination rates of different strains under UV 8h irradiation
Note that different lower case letters in the same column indicate significant differences in germination rates between different strains (P < 0.05) by Tukey's test.
Claims (9)
1. An excellent space returning strain with disease-causing effect on common thistle marigold, which is beauveria bassiana (Beauveria bassiana (balsamo) Vuillemin)Beauveria bassiana) Strain SCAUHT18 preserved in Guangdong province microbial strainThe collection (GDMCC) is deposited as GDMCC NO: 62188.
2. Use of the strain according to claim 1 for field control of cynara scolymus.
3. The use according to claim 2, wherein the strain is cultured to produce a spore liquid or a spore-containing microbial inoculum.
4. The use of claim 2 or 3, wherein the final concentration of Beauveria bassiana when used is 1 x 106~1×109conidia/mL, preferably 1X 107~1×108conidia/mL.
5. Use according to claim 2 or 3, wherein the strain is administered with a spore concentration of 108spores/mL.
6. The use according to claim 2 or 3, wherein the bacterial agent is administered at a time of prediction of the future occurrence or early occurrence of Cirsium japonicum.
7. The use of claim 6, wherein the microbial inoculum is repeatedly applied every 5 to 7 days.
8. A fungicide for controlling common thrips, the active ingredient of which is the strain according to claim 1.
9. The microbial preparation according to claim 8, which is a spore liquid or a spore powder.
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| CN114982769A (en) * | 2022-06-04 | 2022-09-02 | 华南农业大学 | Pesticide containing emamectin benzoate and beauveria bassiana as effective components and application thereof in preventing and treating common thrips |
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| CN110066739A (en) * | 2019-04-25 | 2019-07-30 | 华南农业大学 | A kind of muscardine SB010 and its application in the common setose thistle Malaysia and China of prevention and treatment |
| CN113142244A (en) * | 2021-01-05 | 2021-07-23 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB063 and spinetoram |
| CN113142245A (en) * | 2021-01-05 | 2021-07-23 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB038 and spinetoram |
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| CN113142244A (en) * | 2021-01-05 | 2021-07-23 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB063 and spinetoram |
| CN113142245A (en) * | 2021-01-05 | 2021-07-23 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB038 and spinetoram |
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| CN114736810A (en) * | 2022-04-22 | 2022-07-12 | 浙江泰达作物科技有限公司 | Beauveria bassiana strain with high ultraviolet radiation resistance and directed mutagenesis method and application thereof |
| WO2023202608A1 (en) * | 2022-04-22 | 2023-10-26 | 浙江泰达作物科技有限公司 | Beauveria bassiana strain with high ultraviolet radiation resistance as well as directional mutagenesis method therefor and use thereof |
| CN114982769A (en) * | 2022-06-04 | 2022-09-02 | 华南农业大学 | Pesticide containing emamectin benzoate and beauveria bassiana as effective components and application thereof in preventing and treating common thrips |
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