CN113475532A - Metarhizium anisopliae compound biological insecticide and application thereof - Google Patents
Metarhizium anisopliae compound biological insecticide and application thereof Download PDFInfo
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- CN113475532A CN113475532A CN202110857949.3A CN202110857949A CN113475532A CN 113475532 A CN113475532 A CN 113475532A CN 202110857949 A CN202110857949 A CN 202110857949A CN 113475532 A CN113475532 A CN 113475532A
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- Prior art keywords
- metarhizium anisopliae
- pesticide
- biological pesticide
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- surfactant
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Abstract
The invention discloses a metarhizium anisopliae compound biological insecticide and application thereof, and relates to the technical field of biological insecticides. The active ingredients of the pesticide of the invention are metarhizium anisopliae and sophoricoside flavanone G, wherein the number of conidia of the metarhizium anisopliae and the gram number of the sophoricoside flavanone G are 100-200 hundred million: 0.3 to 0.8. The composite biological pesticide has high pesticidal efficiency, can kill pests quickly, improves the stability of the pesticide, and does not generate drug resistance.
Description
Technical Field
The invention belongs to the technical field of biological insecticides, and particularly relates to a metarhizium anisopliae composite biological insecticide and application thereof.
Background
Metarhizium anisopliae is an entomopathogenic fungus for biological control, has the advantages of no environmental pollution, safety to people and livestock and the like, but the metarhizium anisopliae is low in insecticidal efficiency and long in response time, so that the metarhizium anisopliae is often required to be mixed with other chemical insecticides for use, and the aims of improving the lethality rate of pests and quickly killing the pests can be achieved.
Although the insecticidal efficiency can be improved by using the metarhizium anisopliae and other chemical insecticides in a compounding way, the using amount of the agricultural chemical insecticides in the existing compound insecticides is relatively high, which undoubtedly increases the pollution to the environment and causes pests to generate certain insecticide resistance.
For example, in chinese patent CN200810219992.1, a mixed insecticidal composition of metarhizium anisopliae and metarhizium anisopliae is disclosed, the metarhizium anisopliae is a secondary metabolite secreted by metarhizium anisopliae, the patent utilizes the metarhizium anisopliae to realize the synergistic effect on the metarhizium anisopliae, and can solve the problems of unstable prevention and treatment effect and slow effect of the individual metarhizium anisopliae, and improve the efficiency of pest prevention and treatment.
In Chinese patent CN201210341679.1, an insecticidal composition containing metarhizium anisopliae and tefluthrin is disclosed, wherein tefluthrin is a synthetic insecticide, and tests prove that the insecticidal efficiency can be improved by using the metarhizium anisopliae and the tefluthrin for compounding, but the tefluthrin easily causes insects to generate drug resistance, and the compound insecticide is not used for a long time.
Therefore, the development of a composite biological insecticide which can improve the control stability and the insecticidal efficiency of the metarhizium anisopliae insecticide, is not easy to generate drug resistance and is safe to use is a problem to be solved urgently in the industry at present.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the compound biological pesticide of the metarhizium anisopliae, which realizes the synergy of the insecticidal capacity of the metarhizium anisopliae through the sophoricone flavanone G, improves the insecticidal efficiency of the pesticide, takes effect in a time period, and the insects can not generate drug resistance.
The active ingredients in the compound biological pesticide of the metarhizium anisopliae of the invention are metarhizium anisopliae and sophoricoside G, and the metarhizium anisopliae and the sophoricoside G are mixed according to the following proportion:
the number of conidia of the metarhizium anisopliae and the gram number of the sophoraflavone G are 100 hundred million to 200 hundred million: 0.3 to 0.8.
The pesticide of the invention also comprises an adjuvant. In some embodiments the adjuvant is a surfactant, carrier, dispersant, stabilizer.
Preferably, the surfactant of the present invention is an anionic surfactant or a nonionic surfactant. In some embodiments the anionic surfactant is calcium dodecylbenzene sulfonate or phenethylphenol polyoxyethylene ether phosphate.
In some embodiments the nonionic surfactant is castor oil polyoxyethylene ether or phenethylphenol polyoxyethylene ether.
Preferably, the carrier is talcum powder, activated carbon or attapulgite.
Preferably, the dispersant of the present invention is calcium lignosulfonate, polyacrylic acid or sodium dibutylnaphthalene sulfonate.
Preferably, the stabilizer of the present invention is ethylene glycol or isobutanol.
In some embodiments, the pesticide of the present invention comprises the following raw materials by mass:
30 to 70 percent of active ingredients,
1 to 10 percent of surfactant,
5 to 20 percent of dispersant,
5 to 15 percent of stabilizing agent,
the balance being carriers.
The invention also aims to provide the application of the metarhizium anisopliae compound biological insecticide, and the specific using method is that the insecticide is diluted by water and then sprayed on the leaf surface or root soil of crops to prevent and control insect pests.
Compared with the prior art, the invention has the following beneficial effects:
the composite biological pesticide has high pesticidal efficiency, can kill pests quickly, improves the stability of the pesticide, and does not generate drug resistance.
Detailed Description
The present invention will be further described with reference to the following specific examples. In the following examples, metarhizium anisopliae is metarhizium anisopliae raw powder purchased from Chongqing Juxin bioengineering Co., Ltd (the number of spores in 1g metarhizium anisopliae raw powder is 100 hundred million).
Example 1
A compound biological pesticide of metarhizium anisopliae comprises the following components:
50 wt% of active ingredients (the number of conidia of metarhizium anisopliae and the gram of the sophoricoside G are 100 hundred million: 0.5),
5 weight percent of calcium dodecyl benzene sulfonate,
5 percent by weight of calcium lignosulphonate,
10 wt% of ethylene glycol,
the rest is talcum powder.
Examples 2 to 5
The raw materials in the example 1 are adopted, and the mixture ratio of the components in the example 1 is only adjusted to prepare the metarhizium anisopliae compound biological insecticide, which has the composition shown in the table 1:
TABLE 1
Example 6
A compound biological pesticide of metarhizium anisopliae comprises the following components:
50 percent of active ingredients (the number of conidia of metarhizium anisopliae and the gram of the sophoricoside G are 100 hundred million: 0.5),
5 percent of phenethyl phenol polyoxyethylene ether phosphate ester,
5 percent by weight of calcium lignosulphonate,
10 wt% of ethylene glycol,
the rest is talcum powder.
Example 7
A compound biological pesticide of metarhizium anisopliae comprises the following components:
50 wt% of active ingredients (the number of conidia of metarhizium anisopliae and the gram of the sophoricoside G are 100 hundred million: 0.5),
5 percent of castor oil polyoxyethylene ether,
5 percent by weight of calcium lignosulphonate,
10 wt% of ethylene glycol,
the rest is talcum powder.
Example 8
A compound biological pesticide of metarhizium anisopliae comprises the following components:
50 wt% of active ingredients (the number of conidia of metarhizium anisopliae and the gram of the sophoricoside G are 100 hundred million: 0.5),
5 weight percent of phenethyl phenol polyoxyethylene ether,
5 percent by weight of calcium lignosulphonate,
10 wt% of ethylene glycol,
the rest is talcum powder.
Example 9
A compound biological pesticide of metarhizium anisopliae comprises the following components:
50 wt% of active ingredients (the number of conidia of metarhizium anisopliae and the gram of the sophoricoside G are 100 hundred million: 0.5),
5 weight percent of calcium dodecyl benzene sulfonate,
5 wt% of polyacrylic acid,
10 wt% of ethylene glycol,
the rest is talcum powder.
Example 10
A compound biological pesticide of metarhizium anisopliae comprises the following components:
50 wt% of active ingredients (the number of conidia of metarhizium anisopliae and the gram of the sophoricoside G are 100 hundred million: 0.5),
5 weight percent of calcium dodecyl benzene sulfonate,
5 weight percent of dibutyl sodium naphthalene sulfonate,
10 wt% of ethylene glycol,
the rest is talcum powder.
Example 11
A compound biological pesticide of metarhizium anisopliae comprises the following components:
50 wt% of active ingredients (the number of conidia of metarhizium anisopliae and the gram of the sophoricoside G are 100 hundred million: 0.5),
5 weight percent of calcium dodecyl benzene sulfonate,
5 percent by weight of calcium lignosulphonate,
10wt percent of isobutanol,
the rest is talcum powder.
Example 12
A compound biological pesticide of metarhizium anisopliae comprises the following components:
50 wt% of active ingredients (the number of conidia of metarhizium anisopliae and the gram of the sophoricoside G are 100 hundred million: 0.5),
5 weight percent of calcium dodecyl benzene sulfonate,
5 percent by weight of calcium lignosulphonate,
10 wt% of ethylene glycol,
the balance of activated carbon.
Example 13
A compound biological pesticide of metarhizium anisopliae comprises the following components:
50 wt% of active ingredients (the number of conidia of metarhizium anisopliae and the gram of the sophoricoside G are 100 hundred million: 0.5),
5 weight percent of calcium dodecyl benzene sulfonate,
5 percent by weight of calcium lignosulphonate,
10 wt% of ethylene glycol,
the balance of attapulgite.
Comparative example 1
A metarhizium anisopliae insecticide comprises the following components:
50 wt% of Metarhizium anisopliae
5 weight percent of calcium dodecyl benzene sulfonate,
5 percent by weight of calcium lignosulphonate,
10 wt% of ethylene glycol,
the rest is talcum powder.
Comparative example 2
An insecticide, which comprises the following components:
sophora flavanone G50 wt%
5 weight percent of calcium dodecyl benzene sulfonate,
5 percent by weight of calcium lignosulphonate,
10 wt% of ethylene glycol,
the rest is talcum powder.
Insecticidal Performance testing
Diluting the pesticides prepared in examples 1-13 and comparative examples 1-2 with water, wherein the mass ratio of the pesticides to the water is 1:800, and then detecting the insecticidal effect of the diluted pesticides, wherein the specific detection method comprises the following steps:
respectively soaking filter paper with the diameter of 10cm in the insecticide aqueous solution for wetting, paving the wetted filter paper in a circular culture dish with the diameter of 10cm, soaking fresh tea in the insecticide aqueous solution with the same filter paper for 15s, taking out the tea, paving the tea in the culture dish, gently moving 30 third-instar tea lesser leafhopper larvae with the same size into the culture dish by using a writing brush, repeating the operation for 4 times, and adding clear water for comparison. During the test, the filter paper was replenished with fresh water in time to keep the filter paper wet, and the number of survivors was recorded daily as shown in table 2.
TABLE 2
| 72 h/piece | 96 h/piece | 120 h/only | 144 h/only | |
| Example 1 | 11 | 3 | 1 | 0 |
| Example 2 | 13 | 5 | 3 | 0 |
| Example 3 | 12 | 7 | 5 | 0 |
| Example 4 | 13 | 6 | 2 | 0 |
| Example 5 | 13 | 5 | 2 | 0 |
| Example 6 | 12 | 5 | 2 | 0 |
| Example 7 | 12 | 6 | 3 | 0 |
| Example 8 | 10 | 6 | 3 | 0 |
| Example 9 | 11 | 7 | 3 | 0 |
| Example 10 | 12 | 7 | 4 | 0 |
| Example 11 | 14 | 8 | 5 | 0 |
| Example 12 | 13 | 7 | 5 | 0 |
| Example 13 | 15 | 9 | 3 | 0 |
| Comparative example 1 | 26 | 21 | 14 | 10 |
| Comparative example 2 | 22 | 18 | 10 | 7 |
| Blank example | 29 | 26 | 24 | 22 |
As can be seen from Table 2, the insecticides of the present invention have a high killing rate against pests. The pesticide (comparative example 1) prepared by only using metarhizium anisopliae as an active component has long acting time and cannot achieve the effect of killing pests by 100 percent in a short time. Meanwhile, the pesticide prepared by only using the sophoricoside G as an active component cannot achieve the effect of killing hundreds of insects.
The above embodiments are merely examples of the present invention, and it is obvious that the present invention is not limited to the above embodiments, but may be modified. All modifications directly or indirectly derivable by a person skilled in the art from the present disclosure are to be considered within the scope of the present invention.
Claims (9)
1. The compound biological pesticide is characterized in that the active ingredients of the pesticide are metarhizium anisopliae and sophoricoside G, and the metarhizium anisopliae and the sophoricoside G are mixed according to the following proportion:
the number of conidia of the metarhizium anisopliae and the gram number of the sophoraflavone G are 100 hundred million to 200 hundred million: 0.3 to 0.8.
2. The metarhizium anisopliae composite biological pesticide of claim 1, wherein the pesticide further comprises an adjuvant.
3. The metarhizium anisopliae compound biological pesticide of claim 2, wherein the auxiliary agent is a surfactant, a carrier, a dispersant or a stabilizer.
4. The metarhizium anisopliae composite biological pesticide of claim 3, wherein the surfactant is an anionic surfactant or a nonionic surfactant.
5. The metarhizium anisopliae composite biological pesticide of claim 3, wherein the carrier is talcum powder, activated carbon or attapulgite.
6. The metarhizium anisopliae composite biological pesticide of claim 3, wherein the dispersant is calcium lignosulphonate, polyacrylic acid or sodium dibutylnaphthalene sulfonate.
7. The metarhizium anisopliae composite biological pesticide of claim 3, wherein the stabilizer is glycol or isobutanol.
8. The metarhizium anisopliae compound biological pesticide of claim 7, which is characterized by comprising the following raw materials in percentage by mass:
30 to 70 percent of active ingredients,
1 to 10 percent of surfactant,
5 to 20 percent of dispersant,
5 to 15 percent of stabilizing agent,
the balance being carriers.
9. The application of the metarhizium anisopliae compound biological pesticide as claimed in any one of claims 1 to 8, wherein the pesticide is diluted by water and then sprayed on the leaf surface or root soil of crops to prevent and control insect pests.
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| CN202110857949.3A CN113475532A (en) | 2021-07-28 | 2021-07-28 | Metarhizium anisopliae compound biological insecticide and application thereof |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0873372A (en) * | 1994-08-31 | 1996-03-19 | Ichimaru Pharcos Co Ltd | Antimicrobial agent and preservative and cosmetic containing artocarpin or/and sophoraflavanone g |
| CN101427691A (en) * | 2008-12-15 | 2009-05-13 | 华南农业大学 | Scarabaeus green muscardine fungus and green muscardine fungus native mixed insecticidal composition |
| WO2009148880A2 (en) * | 2008-05-29 | 2009-12-10 | Dsm Ip Assets B.V. | Antimicrobial polymers and their uses |
| CN102823625A (en) * | 2012-09-14 | 2012-12-19 | 广东省昆虫研究所 | Insecticidal composition containing metarhizium anisopliae and tefluthrin |
| CN106265628A (en) * | 2016-07-18 | 2017-01-04 | 暨南大学 | Sophoraflavanone G kills the application in Fish ectoparasite ciliate medicine in preparation |
| CN109221115A (en) * | 2018-10-10 | 2019-01-18 | 广西中医药大学 | A kind of pesticide made of Chinese medicinal herbs of antibacterial desinsection |
| KR20200030863A (en) * | 2018-09-13 | 2020-03-23 | 경희대학교 산학협력단 | An antimicrobial composition comprising sophoraflavanone g as an active ingredient |
| CN111771889A (en) * | 2020-08-03 | 2020-10-16 | 遵义师范学院 | Metarhizium anisopliae drug composition applied to tea garden pest control and application |
-
2021
- 2021-07-28 CN CN202110857949.3A patent/CN113475532A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0873372A (en) * | 1994-08-31 | 1996-03-19 | Ichimaru Pharcos Co Ltd | Antimicrobial agent and preservative and cosmetic containing artocarpin or/and sophoraflavanone g |
| WO2009148880A2 (en) * | 2008-05-29 | 2009-12-10 | Dsm Ip Assets B.V. | Antimicrobial polymers and their uses |
| CN101427691A (en) * | 2008-12-15 | 2009-05-13 | 华南农业大学 | Scarabaeus green muscardine fungus and green muscardine fungus native mixed insecticidal composition |
| CN102823625A (en) * | 2012-09-14 | 2012-12-19 | 广东省昆虫研究所 | Insecticidal composition containing metarhizium anisopliae and tefluthrin |
| CN106265628A (en) * | 2016-07-18 | 2017-01-04 | 暨南大学 | Sophoraflavanone G kills the application in Fish ectoparasite ciliate medicine in preparation |
| KR20200030863A (en) * | 2018-09-13 | 2020-03-23 | 경희대학교 산학협력단 | An antimicrobial composition comprising sophoraflavanone g as an active ingredient |
| CN109221115A (en) * | 2018-10-10 | 2019-01-18 | 广西中医药大学 | A kind of pesticide made of Chinese medicinal herbs of antibacterial desinsection |
| CN111771889A (en) * | 2020-08-03 | 2020-10-16 | 遵义师范学院 | Metarhizium anisopliae drug composition applied to tea garden pest control and application |
Non-Patent Citations (2)
| Title |
|---|
| YAMAMOTO, H: "Origin of two isoprenoid units in a lavandulyl moiety of sophoraflavanone G from Sophora flavescens cultured cells", 《PHYTOCHEMISTRY》 * |
| 郑永权等: "苦参杀虫抑菌活性成分研究", 《农药学学报》 * |
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