CN109221115A - A kind of pesticide made of Chinese medicinal herbs of antibacterial desinsection - Google Patents
A kind of pesticide made of Chinese medicinal herbs of antibacterial desinsection Download PDFInfo
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- CN109221115A CN109221115A CN201811175799.2A CN201811175799A CN109221115A CN 109221115 A CN109221115 A CN 109221115A CN 201811175799 A CN201811175799 A CN 201811175799A CN 109221115 A CN109221115 A CN 109221115A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/26—Meliaceae [Chinaberry or Mahogany family], e.g. mahogany, langsat or neem
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/30—Polygonaceae [Buckwheat family], e.g. red-knees or rhubarb
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
- A01N65/385—Tobacco
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
It is the microemulsion made of ingredient A and ingredient B and suitable surfactant, cosolvent, defoaming agent the invention discloses a kind of pesticide made of Chinese medicinal herbs of antibacterial desinsection；The ingredient A is active insecticidal components, for the toosendanin in polygonum flaccidum dialdehyde, azedarach, periploca spium root chloroform extract, the combination for not toasting tobacco chloroform extract, thunder godvine alkaloid extract, kuh-seng alkaloid, Azalea pontica extract, any one or any n kind in madder ethanol extract, 2≤n≤8；The ingredient B is bacteriostatic active ingredients, for the combination of any one or any m kind in the petroleum ether extract of volatile oil, periploca spium stem in Herba polygoni hydropiperis extract, Folium Meliae, tobacco bud essential oil, the ethanol extract of tripterygium wilfordii, rubimaillin, the ethanol extract of kuh-seng, Azalea pontica general flavone extracting solution, 2≤m≤8.
Description
Technical field
The invention belongs to Chinese herbal medicine and technical field of pesticide, and in particular to and one kind is capable of the pesticide made of Chinese medicinal herbs of antibacterial desinsection,
Safely and effectively new pesticide is provided for the plantation generation of crops.
Background technique
According to major agribusiness's situation of selling well agricultural product data, market current hundred obtained by Agrochemicals status and market survey
That all there is sphere of actions is small for/eight ten or more Pesticidal products, effect toxic to human body and has residual to crops.
The applicant has found that Guangxi is many distinctive and the Chinese herbal medicine grown extensively has very in study and practice process
Good antibacterial insecticidal effect.The antibacterial insecticidal effect of this kind of Chinese herbal medicine and no less than current general pesticide, and be eco-friendly pesticide, both
It ensure that the toxic action of pesticide, and harmless protective to peasant household's body.Pesticide is used in order to more preferably understand national conditions and people
Situation, our several villages and small towns in on-site inspection Nanning City and more than 200 agricultural chemicals enterprises understand Pesticide use situation, and integrative medicine
With pharmacy knowledge, develops kind of a formula rate for the green Chinese herbal medicine pesticide of antibacterial desinsection, raw material extracting method and be somebody's turn to do
The preparation method of pesticide.
Summary of the invention
Goal of the invention: researching and developing a kind of pesticide made of Chinese medicinal herbs of antibacterial desinsection, low in raw material price, the Traditional Chinese medicine bacteriostatic screened,
Active insecticidal components are easy to extract, and are conducive to development of resources and utilize；Compared with current chemical pesticide, except with general plant source agriculture
Also there is efficient proportion mode, stiff stability and the safety to human health outside medicine popular feature.
Technical solution: the purpose of the present invention is what is realized by following technical proposals:
A kind of pesticide made of Chinese medicinal herbs of antibacterial desinsection, be by ingredient A and ingredient B and suitable surfactant, cosolvent,
Microemulsion made of defoaming agent；
The ingredient A is active insecticidal components, is polygonum flaccidum dialdehyde, the toosendanin in azedarach, the extraction of periploca spium root chloroform
Object does not toast tobacco chloroform extract, thunder godvine alkaloid extract, kuh-seng alkaloid, Azalea pontica extract (FC-22), madder
The combination of any one or any n kind in careless ethanol extract, 2≤n≤8；
The ingredient B is bacteriostatic active ingredients, is the petroleum ether of Herba polygoni hydropiperis extract, the volatile oil in Folium Meliae, periploca spium stem
Extract, tobacco bud essential oil, the ethanol extract of tripterygium wilfordii, rubimaillin, the ethanol extract of kuh-seng, Azalea pontica are always yellow
The combination of any one or any m kind in ketone extracting solution, 2≤m≤8；
The Herba polygoni hydropiperis extract is that polygonum flaccidum methanolic extract or polygonum flaccidum ligroin extraction or polygonum flaccidum ethyl acetate extract
Object；
The cosolvent is n-butanol, ethyl alcohol, preferably ethyl alcohol；
For the pesticide made of Chinese medicinal herbs using desinsection as primary efficacy, the weight ratio of the ingredient A and the ingredient B are 5:1；
Under normal circumstances, in order to test and match conveniently, wherein in the inside n kind constituent of the ingredient A, each group
Weight at ingredient is the 1/n of ingredient A total weight, it may be assumed that if ingredient A includes 2 kinds of constituents, then every kind of constituent accounts for
The 1/2 of total weight；If ingredient A includes 3 kinds of constituents, then every kind of constituent accounts for the 1/3 of total weight, pushes away class with this；Institute
It states in the m kind constituent of ingredient B, the weight of each constituent is the 1/m of ingredient B total weight.
Preferably, the equal value 8 of n and m, ingredient A are extracted by toosendanin, the periploca spium root chloroform in polygonum flaccidum dialdehyde, azedarach
Object does not toast tobacco chloroform extract, thunder godvine alkaloid extract, kuh-seng alkaloid, Azalea pontica extract (FC-22), madder
Careless ethanol extract composition, each constituent account for the 1/8 of ingredient A total weight；In ingredient B, the Herba polygoni hydropiperis extract is selected peppery
Knotweed ethyl acetate extract, and the petroleum ether extract of the volatile oil, periploca spium stem in polygonum flaccidum ethyl acetate extract, Folium Meliae,
Tobacco bud essential oil, the ethanol extract of tripterygium wilfordii, rubimaillin, the ethanol extract of kuh-seng, Azalea pontica general flavone extract
Liquid respectively accounts for the 1/8 of ingredient B total weight.
It is furthermore preferred that it is directed to planthopper, rice leaf roller, and in ingredient A, toosendanin, periploca spium in polygonum flaccidum dialdehyde, azedarach
Root chloroform extract, do not toast tobacco chloroform extract, thunder godvine alkaloid extract increases by 30% on the original basis,
Its ratio for respectively accounting for ingredient A total weight are as follows: 1/8* (1+30%)=16.25%, and kuh-seng alkaloid, Azalea pontica extract (FC-
22) it is (1-16.25%*5)/3=6.25% that, madder ethanol extract, which respectively accounts for the ratio of ingredient A total weight,；It is described in ingredient B
Herba polygoni hydropiperis extract selects peppery few ethyl acetate extract, the peppery few ethyl acetate extract, the volatile oil in Folium Meliae, periploca spium
The petroleum ether extract of stem, tobacco bud essential oil, the ethanol extract of tripterygium wilfordii, rubimaillin, kuh-seng ethanol extract,
Azalea pontica general flavone extracting solution respectively accounts for the 1/8 of ingredient B total weight.
It is furthermore preferred that for sugarcane moth borer and continuous worm, aphid, diamondback moth, in ingredient A, the river in polygonum flaccidum dialdehyde, azedarach
Chinaberry element, does not toast tobacco chloroform extract, thunder godvine alkaloid extract, kuh-seng alkaloid, Huang at periploca spium root chloroform extract
Cuckoo extract (FC-22), madder ethanol extract respectively account for the 1/8 of ingredient A total weight；In ingredient B, the Herba polygoni hydropiperis extract choosing
With peppery few ethyl acetate extract, and the ethanol extract of the ethanol extract of tripterygium wilfordii, rubimaillin, kuh-seng, Azalea pontica
General flavone extracting solution increases by 25% on the original basis, respectively account for ingredient B total weight ratio be 1/8* (1+25%)=
15.625%, and peppery few ethyl acetate extract, petroleum ether extract, the tobacco bud of volatile oil, periploca spium stem in Folium Meliae
The ratio that essential oil respectively accounts for ingredient B total weight is (1-15.625%*4)/4=9.375%.
The preparation method of the pesticide made of Chinese medicinal herbs of the antibacterial desinsection, includes the following steps:
(1) with Rotary Evaporators by the supernatant of the ingredient A and ingredient B ethyl alcohol remove, obtain the ingredient A and
The oil components of ingredient B；
(2) surfactant of appropriate hydrophilic and oleophilic value (HLB) is selected, the amount of the surfactant is oil components
2-5 times of volume；The surfactant uses anionic/non-ionic/nonionic compound, and the anionic selects rouge
Fat alcohol polyoxyethylene ether, non-ionic selected fatty acid polyoxyethylene base ester and alkylaryl sulfonates, and fatty alcohol polyoxy
Vinyl ethers: aliphatic acid polyethenoxy base ester: alkylaryl sulfonates=1:1:1, so that the surfactant is in compounding
After can play synergistic effect, HLB value also occurs to change accordingly, and the formation of microemulsion is more stable, transparency temperature model
It encloses and also accordingly broadens；
(3) suitable cosolvent, defoaming agent are selected, the cosolvent mainly has n-butanol, ethyl alcohol etc., it is contemplated that it is economical because
Chinese medicine mixed extract in element and this experiment is extracted with ethyl alcohol, and solubility in ethanol is bigger, and ethyl alcohol itself
There is certain fungistatic effect, reinforce the pesticide fungistatic effect, therefore, cosolvent selects ethyl alcohol；The suitable defoaming agent of reselection；
(4) after determining the dosage of surfactant, the compound proportion of surfactant by above-mentioned steps, with surfactant
Dosage, the compound proportion of surfactant, the dosage of cosolvent ethyl alcohol, defoaming agent dosage be 4 factors, with microemulsion
Cloud point is inspection target, selects optimal prescription, is prepared into microemulsion, and sealing room temperature is kept in dark place；The cloud point refers to microemulsion
In being gradually increasing from the muddy average value for becoming clear temperature or the temperature become cloudy by clarification with temperature.
The advantages of the present invention: the Chinese herbal medicine new pesticide that the present invention researches and develops is mainly in recent years high-incidence agriculture
Crop disease and insect provides safely and effectively new pesticide for the plantation of crops；Used herbal raw material is cheap,
Guangxi or even whole nation growth extensively, meet peasant household's requirement, conducive to the reasonable development and utilization of resource；The Traditional Chinese medicine bacteriostatic screened,
Active insecticidal components are easy to extract, and extracted amount is higher, resource rational utilization；Selective preparation can be done, it can be according to various regions species of insect pests
The difference of class, selectivity are made the pesticide for being suitble to locality with various concentration and ratio, pesticide are made to have more specific aim to its pest；
Its function and effect also has most efficient proportion compared with current chemical pesticide in addition to general botanical pesticide popular feature
Mode, stiff stability and the advantages of to the Novel Chinese herbal medicines pesticide such as safety of human health, also have quality secure, active
Component content is clear, adapts to modern society's healthy living theory, is easy to the advantages that developing and using；And break previous pesticide cannot be
The habit of edible phase sprinkling, conducive to antibacterial to reach fresh-keeping purpose in vegetable and fruit transport process.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, combined with specific embodiments below to the present invention
It is described in detail.
One, the extracting method and step of ingredient A and each constituent of ingredient B:
1, ingredient A:
(1) peppery few, extract is polygonum flaccidum dialdehyde, and fresh peppery trailing plants plant extracting method: is placed on ventilation, shading, thousand
It dries in dry place.It takes 2500 grams of leaves to smash to pieces, is impregnated one week with 10 liters of anhydrous ethers.Soak is filtered out, a small amount of nothing of residue
The elution of water ether, leacheate merge with filtrate, and then plus people's anhydrous sodium sulfate is 48 hours dry.Removed with thin film evaporator volatilization
Whole solvents obtain 16.5 grams of blackish greens and glue cream, are the 0.66% of leaf weight, weigh peppery few 0.5 3 grams of ether extract, use
100 grams of silica gel (60-100 mesh) carry out Gradient column chromatography with hexane/ether.It is monitored using thin plate chromatography, few two will be contained
The part of aldehyde merges, and after removing solvent, obtains 0.33 gram of crude product.If method carries out primary column chromatography again, make standard specimen with synthetic sample, passes through
It is 0.064 gram that GC, which examines peppery few dialdehyde content, so that the content for calculating the peppery few dialdehyde in ether extract is
12.17%, and the content in peppery few cured leaf be about 0.08% (amount being adsorbed in chromatographic column to count).
(2) China tree, extract China tree water, China tree water boil liquid (useful component is the toosendanin in azedarach) extraction side
Method: claiming smashed sample 25g, adds 150mL water, impregnates 60h, then heats and boils 0.5h, cooled and filtered, and filtrate water is fixed
Hold to 125mL, dry-eye disease containing 0.2g in suitable 1mL solution is placed in 8 DEG C of refrigerators and saves backup.
(3) periploca spium, extract are chloroform extract, extracting method: claim smashed periploca spium root skin powder 50g, with filter
Paper bag is filled in Soxhlet extractor at tubbiness, is added 200mL chloroform recovery solvent soaking 12h, is then extracted 6h, flows back 5 per hour
Secondary, extracting solution evaporates on the rotary evaporator closely to be done, and residue is dissolved with 93% ethyl alcohol and is settled to 50mL for gained extracting solution
It is put into 8 refrigerators and saves backup (concentration is equivalent to the 1mL solvent root skin of periploca spium containing 1g powder).
(4) tobacco is not toasted, and extract is chloroform extracted solution.Chloroform extracted solution extracting method: claim smashed sample
50g (tobacco sample 25g) is bundled into tubbiness with filter paper, in Serb Soxhlet extractor, with 200mL chloroform recovery solvent soaking 12h,
Then extracting 6h, Extracting temperature are 85 DEG C, are flowed back 5 times per hour, extracting solution is concentrated under reduced pressure in rotary evaporator, gained chloroform
Extract is dissolved with 95% ethyl alcohol and is settled to 50mL.Extract concentration is equivalent to dry-eye disease containing 1g in 1mL solution, and (tobacco is
0.5g/mL), it is placed in 8 DEG C of refrigerators and saves backup.
(5) tripterygium wilfordii, extract are thunder godvine alkaloid.The extracting method of thunder godvine alkaloid: by velamen of Tripterygium wilfordii
It dries at 60 DEG C or less, is crushed into powder with plant pulverizer, impregnate 12h, extract to obtain tripterygium wilfordii leaching three times with alcohol reflux (6h)
Cream tripterygium wilfordii medicinal extract has sediment generation after adding water, after being filtered, dries into the mass fraction 5%HCl that coarse powder is measured with 8 times
The solution diacolation coarse powder, percolate are adjusted to pH9~10 with concentrated NaOH solution, stand for 24 hours, are precipitated with filtered on buchner funnel
Object is washed with water sediment 1 time, then is filtered dry, dry under the conditions of 60 DEG C, obtains thick tripterygium wilfordii total alkali.With ether dissolution, ether liquid quality
The extraction of score 1%HCl solution, extract liquor are adjusted to pH9~10. with ammonium hydroxide and stand overnight, filter out sediment and sediment 2 is washed with water
It is secondary, it is filtered dry, 60 DEG C of dryings obtain white powder product, by this total alkali with acetone recrystallizing methanol to get colourless granules shape total alkali
Sterling.
(6) madder, extract are its ethanol extract, and the extracting method of madder ethyl alcohol: claiming 80g madder herb powder,
It is fitted into 1 000mL conical flask, adds 10 times of 95% ethyl alcohol of amount, soaking at room temperature rear ultrasonic extraction 40min, filtering for 24 hours repeats to extract
3 times, merging filtrate obtains ethanol extract after being concentrated under reduced pressure.Ethanol extract is diluted to series mass concentration with 50% acetone,
It is placed in spare in 4 DEG C of refrigerators.
(7) kuh-seng, extract are kuh-seng alkaloid.The extracting method of kuh-seng alkaloid: seepage pressure effects technique are as follows: kuh-seng
After coarse powder sufficiently impregnates 2 hours with 0.2% aqueous hydrochloric acid solution, using percolation with solid-liquid ratio 1:15, diacolation extraction in 24 hours is bitter
Alkaloid component in ginseng, obtains percolate；It is preferred that going out kuh-seng alkaloid separation, purifying process are as follows: percolate ammonium hydroxide tune pH7,
Precipitating, filtering, then pH9 is adjusted, it precipitates, filtering, filtrate is by large pore resin absorption column, with the absorption of 5BVh-1 speed upper prop.Acid
Property ethanol elution column in kuh-seng alkaloid, matrine to the greatest extent in column is eluted with high-volume fractional acidic ethanol (about 3 times of resin volumes),
After acidic ethanol (about 5 times of resin volumes) elution column internal oxidition matrine and the N-Oxysophocarpine to the greatest extent with low volume fraction.It will be bitter
Ginseng alkali eluent is concentrated under reduced pressure into syrupy shape, and neutral alumina mixes cream and acetone is added to be heated to reflux 2h, and acetone crystallizes up to matrine,
Obtain matrine sterling.
(8) Azalea pontica, extract are as follows: Azalea pontica extract (FC-22).Its extracting method: the first: by Rhododendron molle flowers
It dries under 23~27 DEG C of sunlight or is dried in 40 DEG C or less, obtain methanol (MeOH) extract with reference to the method for Hu Meiying etc..By clock
The method of China of state etc. obtains ethyl acetate (EtOAc) extract, methylene chloride (CH2Cl2) extract, methanol-water respectively
(MeOH-H2O) extract carries out silica gel column chromatography to EtOAc extract with reference to the methods of Klocke, separates to obtain w=81.58%
R-III, prepare for not being converted to sterling when reagent liquid.Second: Azalea pontica is taken into medicine under petroleum ether extracting at room temperature
Slag, then by the dregs of a decoction at room temperature through ether extraction, its dregs of a decoction is taken, then its dregs of a decoction is obtained into chloroform at room temperature through chloroform
Extract (FC), then this chloroform extract is chromatographed by silicagel column, FC-1, FC-2, FC-3, FC-4.FC-5 are obtained, then
By FC-2 and FC-3, FC-4 is merged into FC-2, and using chloroform, aluminium column obtains FC-22.
2, ingredient B:
(1) polygonum flaccidum, extract are polygonum flaccidum ethyl acetate extract, preparation method: are extracted Chinese medicine polygonum flaccidum sample
It sets in Soxhlet extractor respectively, is successively separately added into each 300mL of petroleum ether, ethyl acetate, methanol, flow back 7h, and concentration and recovery is molten
Agent obtains corresponding extract.
(2) Folium Meliae (or kernel), extract are the volatile oil in Folium Meliae.Preparation method: China tree kernel powder is taken
Methanol etc. is added in end, and the effective component of China tree is extracted using ultrasonic technology.Processed light yellow China tree crude extract powder, with
Dimethyl sulfoxide is cosolvent, and the phosphate buffer solution of PH=7.2 is diluent, is configured to China tree liquid.
(3) periploca spium stem, extract are as follows: petroleum ether extract.Preparation method: periploca spium stem thickness powder 8kg is taken, with 95% second
Alcohol soak at room temperature extracts (30LX5), and medicinal extract 219g is concentrated under reduced pressure to obtain in combined extract.Medicinal extract is well-dispersed in distilled water,
With recycling design after petroleum ether, extraction, petroleum ether extract 60g is obtained.
(4) tobacco, extract are tobacco bud essential oil, and preparation method: the sample for weighing certain mass is placed in flask
In, a certain amount of sodium chloride and distilled water is added, enters flask after several beades are connected and fixed after mixing and is placed in ultrasound
Ultrasound is impregnated after a certain period of time in cleaning machine, and flask and steam distillation device are opened electric jacket switch, from distillating drop
Start timing when the first drop out, stop heating after distilling 4h, after cooling, moisture is released from separator lower end.Use liquid-transfering gun
Oil reservoir is collected, and three times with hot water injection's extractor, flushing liquor is merged with oil reservoir, is after cooling again removed lower water, most
Addition anhydrous sodium sulfate thoroughly removes moisture and obtains tobacco bud essential oil afterwards.The essential oil prepared is placed in cold in 4 DEG C of refrigerating chambers
Hiding, it is spare.Under optimum process condition, the tobacco bud extraction of essential oil rate in each place of production is different, Yunnan mountain of papers, south china essence
Oil extract rate is respectively 0.574%, 0.614%.
(5) tripterygium wilfordii, extract be ethanol extract, preparation method: need public affairs rattan be ground into coarse powder, 60 DEG C of dryings
8h.Weighing needs public rattan coarse powder 2008, sets in extractor, 3 times of 75% ethyl alcohol of amount are added, refluxing extraction 1.5h leaching extracting solution is residual
2 times of 75% ethyl alcohol of amount, refluxing extraction 1.5h is respectively added in slag again.Merge extracting solution twice, medicinal carbon 5g is added, stirs evenly, puts
It to room temperature, is filtered with No. III sintered glass funnel, each filtrate splits in 1OOOmL measuring bottle, adds 75% ethyl alcohol to scale, shakes up
It is spare.
(6) madder, extract are rubimaillin, preparation method: using acetone as solvent, accurately weigh that treated
Madder powder 50.0000g, solid-liquid ratio 1:10 are added, and after concussion is extracted for 24 hours in the huge temperature oscillator of 40 0C:J, are filtered by vacuum, rotation
Evaporation, at medicinal extract shape after the interior heating concentration of baking oven, finally being soughed with diformazan family estate, it is spare to be made into lOmg/mL sample.Madder acetone
Final extract yield is 2.67%.
(7) kuh-seng, extract are kuh-seng (Kurarinone) (I) and Sophoraflavanone G (Sophoraflavanone
G) (II) preparation method: weighs radix sophorae of the 200g through crushing and is successively mentioned with n-hexane, ethyl acetate, methanol continuous dipping
Every kind of solvent is taken to be added in three times, each 5OOmL is extracted for 24 hours.Solvent under reduced pressure is evaporated, the extraction of three kinds of solvents is respectively obtained
By document, [4 methods measure mosquitocide active antibacterial activity and eelworm-killing activity wherein to 1.6,10.0 and 27.0g of object, and ethyl acetate mentions
Object is taken to show strong bacteriostatic activity.Under the guidance of bioactivity Tracing Experiment, TLC (silica gel is successively led to ethyl acetate extract
Work MPLC (C-18 } and HPLC (C-18) further isolate and purify, obtain the strongest compound work (2.19g) of two bioactivity
With II (1.03g).
(8) Azalea pontica, extract are Azalea pontica general flavone extracting solution, preparation method: use Waters C18 (4.6mm
× 250mm, 5 μm) reverse-phase chromatographic column, it is eluted in the eluent gradient of first alcohol and water, elution program are as follows: 0-24min, 31%-
42%B；24-30min, 42%-50%B；30-35min, 50%-60%B；
35-40min, 60%B；Detection wavelength 356nm, flow velocity 0.7mL/min can make under the conditions of 30 DEG C of column temperature beautiful
Flavone compound HPLC in cuckoo obtains good separating effect.
Two, Mortality of insect is measured to the bioactive ingredients extracted
Bioassay is carried out using improved skin spy spray-on process.The bioactivity of a variety of extractions is configured by different proportion
Extracting solution, it is on the plant leaf blade of 9cm that medical fluid, which is sprayed onto diameter, by spraying with skin spy, and blade tow sides are both needed to sprinkling medical fluid
1.5mL.After drying, upward by blade reverse side, being transferred to diameter is that 9cm is lined in the culture dish of filter paper.It is then careful with writing brush
It chooses into 10 test pests, 100 cabbage caterpillars that pest picks up from same piece of land use common white dish leaf preculture 3d indoors, so
After be randomly divided into 5 groups, every group 20, be put in 5 30cm x 30cm sand silk screen casees respectively, be 15 husky silk screen casees numbers,
2,3,4,5.The Chinese cabbage leaf that the solution of the beaker of the reference numeral of equivalent impregnates is put to 1,2,3,4, No. 5 husky silk screen case respectively, is fed
Food for 24 hours, is observed, the life survival state of statistics cabbage caterpillar.48h checks death condition after spray, calculates the death rate, and come with this
Evaluate the activity of Chinese herbal medicine (borer population having in fact in culture dish when checking is total as test worm).
Three, pest anti-food rate is measured to the bioactivity extracted
The bioactivity of a variety of extractions is pressed into different proportion configuration extraction liquid, medical fluid, which is sprayed onto diameter, by spraying with skin spy is
On the plant leaf blade of 9cm, blade tow sides are both needed to sprinkling medical fluid 1.5mL.After drying, upward by blade reverse side, it is transferred to straight
Diameter is that 9cm is lined in the culture dish of filter paper.It is then carefully chosen with writing brush into 10 test pests, pest picks up from same piece of land
100 vegetable aphids use common white dish leaf preculture 3d indoors, are then randomly divided into 5 groups, every group 20, are put in 5 30cm respectively
It is 1,2,3,4,55 husky silk screen case numbers in x 30cm sand silk screen case.Equivalent is put to No. 1,2,3,4,5 husky silk screen casees respectively
Reference numeral beaker solution dipping Chinese cabbage leaf, feeding for 24 hours, observation, statistics cabbage caterpillar life survival state.Processing
It is measured for 24 hours with coordinate method by feeding area afterwards, calculates anti-food rate, and Chinese herbal medicine is evaluated to the antifeedant activity of cabbage caterpillar with this.
Death condition is checked after 48h, calculates the death rate.
Four, the harmful bacteria death rate is measured to the bioactive ingredients of taking-up
PDA liquid medium is stand-by by high pressure sterilization processing, Biohazard Safety Equipment ultraviolet disinfection 30 minutes will own
Experimental article be placed on bio-safety counter top, the sickle bought embrace freeze-dried powder peace thus use 75% alcohol absorbent cotton to peace thus outside
Surface carries out disinfection, and heats its top with flame, and the peace event top for dripping a small amount of sterile water to heating is allowed to rupture, with file or tweezer
Son strikes oneself the lower event of the peace through rupturing top and is instilled in peace event with sterile liquid-transfering gun absorption 0.5m1PDA fluid nutrient medium.Gently shake
It swings, makes to be lyophilized thallus dissolution in suspension, draw whole bacteria suspensions, transplant in the triangular pyramidal bottle of 100m1, in 27 DEG C of temperature
Under the conditions of be placed in constant-temperature table, revolving speed 110r/min.Observation bacterium solution state daily is cultivated and is observed under 7d liquid medium within,
Conical flask bottom has a large amount of mycelia to generate, and 10 microlitres of bacterium solutions of suction pipe absorption are placed on coverslip and observe under the microscope, and three
Kind bacterium has different sons of embracing to be formed respectively.Then the method that the bacterium solution of formation is inoculated with to the layout line is seeded on plate, constant temperature
27 DEG C of culture to mycelia are covered in entire plate, as a result, it has been found that covering with bacterium colony in plate after 72h.Three are made a call to punch respectively
Bacteria cake is placed in PDA liquid medium, 27 DEG C of culture 7d of constant-temperature table is placed in, by the bacterium solution shaken out in super-clean bench sterile gauze
Filtering removes mycelia, and being made into bacterial concentration with aqua sterilisa is 0.5-2.5 × 104cfu/ml, takes golden yellow Portugal respectively with liquid-transfering gun
Grape coccus and 50 microlitres of Escherichia coli bacteria liquid are placed in 9cm plate, and PDA solid medium that sterilizing is dissolved is cooled to 45 DEG C,
Plate is injected using sterile working process, each plate 15-20m1 culture medium sets horizontal position and rotates plate rapidly, and mixing is equal
After even, taking diameter is that speckling with for 6mm is placed in culture medium plate by the filter paper for reagent liquid that different proportion configures repeatedly, in
It is cultivated under the conditions of 27 DEG C for 24 hours, observe and measures antibacterial circle diameter with vernier caliper.
Minimal inhibitory concentration (minimum inhibitory concentration, MIC) is measured with 96 orifice plates, in lower layer
100 microlitres of mixed medicine culture mediums are injected, 110 holes are for the doubling dilution of reagent liquid, and 11 holes are negative control, and 12 holes are blank control；
Then configured bacteria suspension is added on upper layer, in constant incubator culture 72h, directly observation as a result, negative control is muddy, hole
If interior turbidity and precipitation occur, that is, shows bacterium well-grown under this condition, then determine in negative hole without precipitating, show without any microorganism
Pollution, the transparent result of blank control is reliable, visually observes transparent hole number, has recorded result.
Five, the harmful bacteria death rate is measured with mtt assay measurement
Terminate preceding 3~4h in incubation, 10 μ L of MTT liquid is added in every hole, after continuing 3~4h of culture, 100 μ L of supernatant is sucked out, adds
Enter 100 μ L of dimethyl sulfoxide (DMSO), after shaking 5min, surveys absorbance A with microplate reader (BioRAD450 type microwell plate interpretoscope)
(measurement wavelength 570nm, reference wavelength 655nm).Equally using be suppressed for 80% hole quality of liquid medicine concentration as effectively mentioning
The MIC for taking ingredient, using the complete repressed minimum drug quality concentration in hole as the MIC terminal for the effective component extracted.It is true
The accuracy and repeatability of experimental result are protected, this experimental result is the reproducible results of 3 experiments.
Six, seek the active appraisal experiment of the optimal proportion (but based on desinsection) of antibacterial desinsection
The effective component that each traditional Chinese medicine extraction is come out individually carries out Mortality of insect and pest anti-food rate respectively first,
And record the killing rate for the specific pest that the effective elements of the medicine in every kind is directed to it
Secondly the mixing (every kind of proportion is identical) of effective extract component of every two kinds of Chinese medicine will be carried out, and is mixed with it
Liquid individually acts on the targeted all pests of both Chinese medicines, measures the death rate and pest anti-food rate of pest, and remembers
Record is got off.
Secondly the mixing (every kind of proportion is identical) of effective extract component of every three kinds of Chinese medicine will be carried out, and is mixed with it
Liquid individually acts on the targeted all pests of both Chinese medicines, measures the death rate and pest anti-food rate of pest, and remembers
Record is got off.
Secondly the mixing (every kind of proportion is identical) of effective extract component of every four kinds of Chinese medicine will be carried out, and is mixed with it
Liquid individually acts on the targeted all pests of both Chinese medicines, measures the death rate and pest anti-food rate of pest, and remembers
Record is got off.
Secondly the mixing (every kind of proportion is identical) of effective extract component of every five kinds of Chinese medicine will be carried out, and is mixed with it
Liquid individually acts on the targeted all pests of both Chinese medicines, measures the death rate and pest anti-food rate of pest, and remembers
Record is got off.
Effective extract component of all Chinese medicine is finally mixed into (every kind of proportion is identical) together, and is mixed with it
Liquid individually acts on the targeted all pests of both Chinese medicines, measures the death rate and pest anti-food rate of pest, and remembers
Record is got off.
According to the death rate and pest anti-food rate for pest obtained above, we can count respectively and can effectively be directed to
Certain collocation (unlimited ingredient is several) of a certain pest effect, and count the death of the ranking of certain pest from high in the end
The ranking of rate and anti-food rate, and first three is taken to arrange in pairs or groups respectively, the higher collocation of the frequency of occurrences is finally counted, as being directed to
The relatively best proportion compatibility of desinsection.Best proportion compatibility in bacteriostatic experiment can also be obtained similarly.
If technical solution part is described previously, the pesticide made of Chinese medicinal herbs using desinsection as primary efficacy, the ingredient A with it is described
The weight ratio of ingredient B is 5:1；
Under normal circumstances, in order to test and match conveniently, wherein in the inside n kind constituent of the ingredient A, each group
Weight at ingredient is the 1/n of ingredient A total weight, it may be assumed that if ingredient A includes 2 kinds of constituents, then every kind of constituent accounts for
The 1/2 of total weight；If ingredient A includes 3 kinds of constituents, then every kind of constituent accounts for the 1/3 of total weight, pushes away class with this；Institute
It states in the m kind constituent of ingredient B, the weight of each constituent is the 1/m of ingredient B total weight.
Preferably, the equal value 8 of n and m, ingredient A are extracted by toosendanin, the periploca spium root chloroform in polygonum flaccidum dialdehyde, azedarach
Object does not toast tobacco chloroform extract, thunder godvine alkaloid extract, kuh-seng alkaloid, Azalea pontica extract (FC-22), madder
Careless ethanol extract composition, each constituent account for the 1/8 of ingredient A total weight；In ingredient B, the Herba polygoni hydropiperis extract is selected peppery
Knotweed ethyl acetate extract, and the petroleum ether extract of the volatile oil, periploca spium stem in polygonum flaccidum ethyl acetate extract, Folium Meliae,
Tobacco bud essential oil, the ethanol extract of tripterygium wilfordii, rubimaillin, the ethanol extract of kuh-seng, Azalea pontica general flavone extract
Liquid respectively accounts for the 1/8 of ingredient B total weight.
It is furthermore preferred that it is directed to planthopper, rice leaf roller, and in ingredient A, toosendanin, periploca spium in polygonum flaccidum dialdehyde, azedarach
Root chloroform extract, do not toast tobacco chloroform extract, thunder godvine alkaloid extract increases by 30% on the original basis,
Then its ratio for respectively accounting for ingredient A total weight are as follows: 1/8* (1+30%)=16.25%, and kuh-seng alkaloid, Azalea pontica extract
(FC-22), it is (1-16.25%*5)/3=6.25% that madder ethanol extract, which respectively accounts for the ratio of ingredient A total weight,；In ingredient B,
The Herba polygoni hydropiperis extract selects polygonum flaccidum ethyl acetate extract, and volatile oil, thick stick in polygonum flaccidum ethyl acetate extract, Folium Meliae
The ethyl alcohol extraction of the petroleum ether extract, tobacco bud essential oil, the ethanol extract of tripterygium wilfordii, rubimaillin, kuh-seng of willow stem
Object, Azalea pontica general flavone extracting solution respectively account for the 1/8 of ingredient B total weight.
It is furthermore preferred that for sugarcane moth borer and continuous worm, aphid, diamondback moth, in ingredient A, the river in polygonum flaccidum dialdehyde, azedarach
Chinaberry element, does not toast tobacco chloroform extract, thunder godvine alkaloid extract, kuh-seng alkaloid, Huang at periploca spium root chloroform extract
Cuckoo extract (FC-22), madder ethanol extract respectively account for the 1/8 of ingredient A total weight；In ingredient B, the Herba polygoni hydropiperis extract choosing
With polygonum flaccidum ethyl acetate extract, and the ethanol extract of the ethanol extract of tripterygium wilfordii, rubimaillin, kuh-seng, Azalea pontica
General flavone extracting solution increases by 25% on the original basis, then its ratio for respectively accounting for ingredient B total weight be 1/8* (1+25%)=
15.625%, and petroleum ether extract, the tobacco bud of volatile oil, periploca spium stem in polygonum flaccidum ethyl acetate extract, Folium Meliae
The ratio that essential oil respectively accounts for ingredient B total weight is (1-15.625%*4)/4=9.375%.
(7) corresponding microemulsion is made and detects its quality standard and primary stability
A, the ethyl alcohol in the supernatant of extracting solution is removed with Rotary Evaporators first；
B, since Chinese medicine mixing alcohol extract is mainly grease, appropriate hydrophilic and oleophilic value (HLB) is selected according to its property
Surfactant, surfactant can play synergistic effect after compounding so that HLB value also occurs to change accordingly,
The formation of microemulsion is more stable, and transparency temperature range also accordingly broadens；In microemulsion common surfactant package be it is non-/
Non-, non-/ negative two states, thus mainly Surfactant proportion is screened in auxiliary material screening, in microemulsion
The amount of surfactant is 2-5 times of oil components；For the mechanical strength and integrality at microemulsion interface, in preliminary screening
The amount that the amount of surfactant can be increased, therefore can fix compound surfactant is 25% progress preliminary screening, and
The mode of non-/ yin compounding is selected to prepare microemulsion during subsequent experimental；The anionic selects aliphatic alcohol polyethenoxy
Base ether, non-ionic selected fatty acid polyoxyethylene base ester and alkylaryl sulfonates, it is auxiliary using the method screening of orthogonal test
Expect the optimal proportion in microemulsion, final proportion compatibility are as follows: yin: non-: non-is 1:1:1, i.e. aliphatic alcohol polyethenoxy base
Ether, aliphatic acid polyethenoxy base ester and alkylaryl sulfonates.
C, microemulsion is mainly by surfactant, cosolvent, and the composition such as defoaming agent, cosolvent mainly has n-butanol, ethyl alcohol
Deng, it is contemplated that the Chinese medicine mixed extract in economic factor and this experiment is extracted with ethyl alcohol, solubility ratio in ethanol
It is larger, and ethyl alcohol itself has certain fungistatic effect, reinforces the pesticide fungistatic effect, therefore, cosolvent selects ethyl alcohol；Reselection is closed
Suitable defoaming agent；
D, after determining the dosage of surfactant, the compound proportion of surfactant by above-mentioned steps, with surfactant
Dosage, the proportion of surfactant, cosolvent ethanol consumption, defoaming agent dosage be 4 factors, each factor is 3 levels,
Mainly with microemulsion cloud point, (cloud point refers to microemulsion as being gradually increasing for temperature becomes clear temperature from muddiness or by clear
The average value of the temperature become cloudy clearly, the i.e. referred to as cloud point of microemulsion) it is inspection target, carry out the screening of optimal prescription；Then
Microemulsion is prepared by optimal kitchen, and measures the content of each active constituent in compound preparation with high performance liquid chromatograph method, is formulated
Simple quality standard, and to it in high temperature, strong light intensity carries out primary stability evaluation in the case of penetrating.
The foregoing is merely presently preferred embodiments of the present invention and related case, is not intended to limit the invention, all in this hair
Made any modifications, equivalent replacements, and improvements etc. within bright spirit and principle, be all contained in protection scope of the present invention it
It is interior.
Claims (9)
1. a kind of pesticide made of Chinese medicinal herbs of antibacterial desinsection, which is characterized in that be by ingredient A and ingredient B and suitable surface-active
Agent, cosolvent, microemulsion made of defoaming agent；
The ingredient A is active insecticidal components, is polygonum flaccidum dialdehyde, the toosendanin in azedarach, periploca spium root chloroform extract, not
Toast tobacco chloroform extract, thunder godvine alkaloid extract, kuh-seng alkaloid, Azalea pontica extract (FC-22), madder ethyl alcohol
The combination of any one or any n kind in extract, 2≤n≤8；
The ingredient B is bacteriostatic active ingredients, is the petroleum ether extraction of Herba polygoni hydropiperis extract, the volatile oil in Folium Meliae, periploca spium stem
Object, tobacco bud essential oil, the ethanol extract of tripterygium wilfordii, rubimaillin, the ethanol extract of kuh-seng, Azalea pontica general flavone mention
Take the combination of any one or any m kind in liquid, 2≤m≤8.
2. the pesticide made of Chinese medicinal herbs of antibacterial desinsection according to claim 1, which is characterized in that the polygonum flaccidum methanolic extract is
Polygonum flaccidum methanolic extract or polygonum flaccidum ligroin extraction or polygonum flaccidum ethyl acetate extract.
3. the pesticide made of Chinese medicinal herbs of antibacterial desinsection according to claim 1, which is characterized in that the cosolvent be n-butanol or
Ethyl alcohol.
4. the pesticide made of Chinese medicinal herbs of antibacterial desinsection according to claim 2, which is characterized in that described in the pesticide made of Chinese medicinal herbs
The weight ratio of the ingredient A and ingredient B is 5:1.
5. the pesticide made of Chinese medicinal herbs of antibacterial desinsection according to claim 4, which is characterized in that the inside n kind group of the ingredient A
At in ingredient, the weight of each constituent is the 1/n of ingredient A total weight；It is each to form in the m kind constituent of the ingredient B
The weight of ingredient is the 1/m of ingredient B total weight.
6. the pesticide made of Chinese medicinal herbs of antibacterial desinsection according to claim 5, which is characterized in that the equal value 8 of n and m；Ingredient A by
Toosendanin, periploca spium root chloroform extract in polygonum flaccidum dialdehyde, azedarach do not toast tobacco chloroform extract, tripterygium wilfordii biology
Alkali extract, kuh-seng alkaloid, Azalea pontica extract, madder ethanol extract composition, each constituent account for ingredient A total weight
1/8；In ingredient B, the Herba polygoni hydropiperis extract selects polygonum flaccidum ethyl acetate extract, and polygonum flaccidum ethyl acetate extract, China tree
The petroleum ether extract of volatile oil, periploca spium stem in leaf, tobacco bud essential oil, the ethanol extract of tripterygium wilfordii, rubimaillin,
Ethanol extract, the Azalea pontica general flavone extracting solution of kuh-seng respectively account for the 1/8 of ingredient B total weight.
7. the pesticide made of Chinese medicinal herbs of antibacterial desinsection according to claim 4, which is characterized in that the equal value 8 of n and m；Fly for rice
Lice, rice leaf roller, in ingredient A, toosendanin in polygonum flaccidum dialdehyde, azedarach, does not toast tobacco at periploca spium root chloroform extract
Chloroform extract, thunder godvine alkaloid extract increase by 30% on the original basis, respectively account for the ratio of ingredient A total weight
Are as follows: 1/8* (1+30%)=16.25%, and kuh-seng alkaloid, Azalea pontica extract, that madder ethanol extract respectively accounts for ingredient A is total
The ratio of weight is (1-16.25%*5)/3=6.25%；In ingredient B, the Herba polygoni hydropiperis extract selects polygonum flaccidum ethyl acetate to mention
Take object, and the petroleum ether extract of the volatile oil, periploca spium stem in polygonum flaccidum ethyl acetate extract, Folium Meliae, tobacco bud essential oil,
The ethanol extract of tripterygium wilfordii, rubimaillin, the ethanol extract of kuh-seng, that Azalea pontica general flavone extracting solution respectively accounts for ingredient B is total
The 1/8 of weight.
8. the pesticide made of Chinese medicinal herbs of antibacterial desinsection according to claim 4, which is characterized in that the equal value 8 of n and m；For sugarcane
Snout moth's larva and continuous worm, aphid, diamondback moth, in ingredient A, toosendanin, periploca spium root chloroform extract in polygonum flaccidum dialdehyde, azedarach,
Tobacco chloroform extract, thunder godvine alkaloid extract, kuh-seng alkaloid, Azalea pontica extract, madder ethyl alcohol is not toasted to extract
Object respectively accounts for the 1/8 of ingredient A total weight；In ingredient B, the Herba polygoni hydropiperis extract selection polygonum flaccidum ethyl acetate extract, and tripterygium wilfordii
Ethanol extract, rubimaillin, the ethanol extract of kuh-seng, Azalea pontica general flavone extracting solution increase on the original basis
25%, the ratio for respectively accounting for ingredient B total weight is 1/8* (1+25%)=15.625%；Polygonum flaccidum ethyl acetate extract, China tree
The ratio that petroleum ether extract, the tobacco bud essential oil of volatile oil, periploca spium stem in leaf respectively account for ingredient B total weight is (1-
15.625%*4)/4=9.375%.
9. according to claim 1 in -8 the pesticide made of Chinese medicinal herbs of any antibacterial desinsection preparation method, which is characterized in that including
Following steps:
(1) ethyl alcohol in the supernatant of the ingredient A and ingredient B is removed with Rotary Evaporators, obtains the ingredient A and ingredient
The oil components of B；
(2) select the surfactant of appropriate hydrophilic and oleophilic value: the amount of the surfactant for oil components volume 2-5
Times；The surfactant uses anionic/non-ionic/nonionic compound, and the anionic selects poly alkyl alcohol
Oxyethylene group ether, non-ionic selected fatty acid polyoxyethylene base ester and alkylaryl sulfonates, and aliphatic alcohol polyethenoxy base
Ether: aliphatic acid polyethenoxy base ester: alkylaryl sulfonates=1:1:1, so that the surfactant can after compounding
Play synergistic effect, HLB value also occurs to change accordingly, and the formation of microemulsion is more stable, transparency temperature range also phase
It should broaden；
(3) select suitable cosolvent and defoaming agent: the cosolvent mainly has n-butanol, ethyl alcohol etc., it is contemplated that economic factor
And the Chinese medicine mixed extract in this experiment is extracted with ethyl alcohol, solubility in ethanol is bigger, and ethyl alcohol itself has
Certain fungistatic effect reinforces the pesticide fungistatic effect, and therefore, cosolvent selects ethyl alcohol；The suitable defoaming agent of reselection；
(4) after determining the dosage of surfactant, the compound proportion of surfactant by above-mentioned steps, with the use of surfactant
Amount, the compound proportion of surfactant, the dosage of cosolvent ethyl alcohol, defoaming agent dosage are 4 factors, with microemulsion cloud point
For inspection target, optimal prescription is selected, is prepared into microemulsion, sealing room temperature is kept in dark place；The cloud point refer to microemulsion with
Being gradually increasing from the muddy average value for becoming clear temperature or the temperature become cloudy by clarification for temperature.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113475532A (en) * | 2021-07-28 | 2021-10-08 | 广州毅田生物技术有限公司 | Metarhizium anisopliae compound biological insecticide and application thereof |
| CN113925067A (en) * | 2021-10-22 | 2022-01-14 | 重庆御尚生物科技有限公司 | Chinese herbal medicine preparation for preventing and treating wax moth and preparation method thereof |
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|---|---|---|---|---|
| CN103704281A (en) * | 2013-12-27 | 2014-04-09 | 成都新朝阳作物科学有限公司 | Pesticide composition containing sophora flavescens and tobacco extracts as well as preparation method and application thereof |
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2018
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103704281A (en) * | 2013-12-27 | 2014-04-09 | 成都新朝阳作物科学有限公司 | Pesticide composition containing sophora flavescens and tobacco extracts as well as preparation method and application thereof |
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| 刘广文: "《现代农药剂型加工技术》", 31 January 2013, 化学工业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113475532A (en) * | 2021-07-28 | 2021-10-08 | 广州毅田生物技术有限公司 | Metarhizium anisopliae compound biological insecticide and application thereof |
| CN113925067A (en) * | 2021-10-22 | 2022-01-14 | 重庆御尚生物科技有限公司 | Chinese herbal medicine preparation for preventing and treating wax moth and preparation method thereof |
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