CN101433215A - Compositional main pesticide and pesticide containing destruxins of Metarhizium anisopliae and high-efficient cypermethrin - Google Patents
Compositional main pesticide and pesticide containing destruxins of Metarhizium anisopliae and high-efficient cypermethrin Download PDFInfo
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- CN101433215A CN101433215A CNA2008102199989A CN200810219998A CN101433215A CN 101433215 A CN101433215 A CN 101433215A CN A2008102199989 A CNA2008102199989 A CN A2008102199989A CN 200810219998 A CN200810219998 A CN 200810219998A CN 101433215 A CN101433215 A CN 101433215A
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Abstract
The invention discloses a pesticidal main agent and a pesticide by compounding destruxins of Metarrhizium anisopliae and high-efficiency cypermethrin. The mass ratio of the destruxins of Metarrhizium anisopliae to the high-efficiency cypermethrin in the main agent is 7-1:3-9. The invention adopts pyrethroid pesticide with high efficiency, low toxicity, low residue and broad spectrum activity, namely the high-efficiency cypermethrin, and entomopathogenic fungi with obvious disinsection effect, namely the destruxins of Metarrhizium anisopliae, for compounding to obtain the pesticidal main agent, and determines the preferable compounding ratio of both through a large number of experiments. The pesticide has brand-new action mechanism different from the prior pesticides and obvious disinsection effect, particularly has obvious toxic action on prodenia litura which has severe harm, and has the characteristic of environmental protection at the same time.
Description
Technical field
The invention belongs to biological pesticide technical field, relate to a kind of destruxins and efficient cypermethrin compounded desinsection host and insecticide.
Background technology
Prodenia litura (Spodoptera litura) is the important pest of a kind of universal omnivorousness, and the frequency in China's big generation in many areas obviously improved in the last few years, and harm increases the weight of.Because the long-term chemical pesticide control that uses, cause this worm that insecticides such as organochlorine, organic phosphor, carbamate and pyrethroid have been produced pesticide resistance in various degree, the most conventional effect of chemical control significantly descends, and can not effectively control its harm, the control difficulty.Efficient beta-cypermethrin is one of important medicament of preventing and treating prodenia litura, because long-term the use developed immunity to drugs at present, therefore suddenly waits to develop effective complex preparation.
Beta-cypermethrin (alpha-Cypermethrin, high chlorine) be at present widely used efficient, low toxicity, low-residual, broad spectrum activity pyrethroid insecticides, have good tagging and stomach poison function, be mainly used in control Lepidoptera, coleoptera blade face insect, be a kind of in the vegetable-crop pest-insect control widely used efficient, low toxicity, low-residual chemical pesticide.Yet insect has greatly been limited its application to the pesticide resistance significantly development of beta-cypermethrin at present.Adopting the agriculture chemical compounding technology or mixing application technique is to overcome or delay a drug-fast important method, and therefore, the Compositional type insecticide of exploitation beta-cypermethrin is significant.Though efficient cypermethrin compounded dose of China's registration is many, for example beta-cypermethrin and pyrethroid, carbamates, organic phosphates, antibiotics and growth regulator class principal item all have mix preparation, but insect is constantly development to the pesticide resistance of agricultural chemicals, and Recompounded pesticide has also constantly been proposed new demand for development.
Destruxins (destruxin) is an insect pathogenic fungus---the cyclic depsipeptides antibiotic that green muscardine fungus (Metarhiziumanisopliae var.anisopliae) produces.Destruxins has significant insecticidal effect, and has the brand-new mechanism of action that is different from existing insecticide, mainly acts on the hemolymph immune system of insect, makes it to lose the immune defense ability.Destruxins is easily degraded, noresidue, and toxicity is low, and the development and use destruxins has important economic implications.
Do not see at present the report of composite dose of beta-cypermethrin and destruxins, development beta-cypermethrin and destruxins compounded pesticides have great importance to pest control, developing agricultural production, especially develop composite dose of significant and economic worth of the beta-cypermethrin and the destruxins that are used to prevent and treat prodenia litura.
Summary of the invention
An object of the present invention is to overcome the deficiencies in the prior art, a kind of destruxins and efficient cypermethrin compounded desinsection host are provided, by continuous experimental study, find the suitable compound proportion of destruxins and beta-cypermethrin, obtain a kind of Compositional type desinsection host of efficient, low toxicity.
Another object of the present invention is on the basis of above-mentioned desinsection host, further determines the proportioning of host and assistant agent, prepares good disinsection effect and the insecticide stable, that the practical application generalization is very strong.
Purpose of the present invention realizes by following technical proposals:
A kind of destruxins and efficient cypermethrin compounded desinsection host are provided, and the mass ratio of described destruxins and beta-cypermethrin is 7～1: 3～9.
The mass ratio of described destruxins and beta-cypermethrin is preferably 3～4: 7～6.
More preferred, in the host provided by the invention, the mass ratio of described destruxins and beta-cypermethrin is 7: 3,5: 5,4: 6,3: 3 or 1: 9.
Described destruxins is any one or the two or more mixture that mixes with any ratio among destruxins A, destruxins B or the destruxins E.
On the basis of above-mentioned host, the present invention specifically provides a kind of insecticide, comprises host and the conventional assistant agent that uses, by mass percentage, host accounts for 20%, and assistant agent accounts for 80%, and described host is that destruxins is the mixture that mixes at 7: 3 according to mass ratio with beta-cypermethrin.
Adopt conventional preparation method described insecticide can be prepared formulations such as becoming emulsifiable concentrate.
The invention has the beneficial effects as follows:
The invention provides the compounded technology scheme of destruxins and beta-cypermethrin, utilized efficient, low toxicity, low-residual, broad spectrum activity pyrethroid insecticides beta-cypermethrin and had the active ingredient of the insect pathogenic fungus destruxins of remarkable insecticidal effect, determined the preferred compound proportion of the two by a large amount of experiments as compounded pesticides; Insecticide of the present invention has the brand-new mechanism of action that is different from existing insecticide, and insecticidal effect is remarkable, and especially the toxic action at the serious prodenia litura of harm is remarkable, has eco-friendly characteristics simultaneously, and the preparation method is simple, is easy to promote.
Embodiment
Always further describe the present invention below in conjunction with concrete enforcement.
Embodiment 1 destruxins of the present invention and efficient cypermethrin compounded host are to the Toxicity Determination experiment of prodenia litura
During Toxicity Determination, according to destruxins and beta-cypermethrin mass ratio is respectively to measure an amount of medicament at 7～1: 3～9, be made into certain density mother liquor with acetone and place refrigerator (4 ℃) standby, all medicaments are all used 0.01% Tween-80 solution dilution during test.Described destruxins is any one or the two or more mixture that mixes with any ratio among destruxins A, destruxins B or the destruxins E.Each is handled 3 times and repeats, and each repeats 1 culture dish, each culture dish put 15 three age Spodoptera litura larvae.At first cabbage leaves is cut into the fritter of 6～7cm, takes out behind the about 5s of immersion soup, after indoor drying, again blade is put into the culture dish that is connected to prodenia litura, preserve moisture with wet cotton balls.Every 24h changes cabbage leaves and clears up culture dish behind the medicine.Experiment repeats secondary.Behind medicine 24,48,72h observed and recorded prodenia litura survival condition, calculate lethality, corrected mortality, and the lethal concentration of 50 (LC of 72h after the computing
50), correlation coefficient and regression equation, calculate the co-toxicity coefficient (CTC) of intermixture with reference to the abundant method of Sun Yun.Because experimental technique belongs to this area conventional method, experimental result is not given unnecessary details one by one, part of test results sees Table 1, by the visible destruxins of table 1 and beta-cypermethrin so that ratio was mixed in 7: 3,5: 5,4: 6,3: 3,3: 7,1: 9, its co-toxicity coefficient reaches 122.26,129.05,138.83,149.86 and 137.40, has synergistic effect.
Table 1 destruxins, beta-cypermethrin and compounded pesticides of the present invention are to the toxicity test of prodenia litura 3 instar larvaes
Illustrate: co-toxicity coefficient CTC had the effect of subtracting effect less than 80 o'clock between the two; Greater than having synergistic effect at 120 o'clock; It in the time of between 80～120 summation action.
(destruxins: the mass ratio of beta-cypermethrin=3: 7) missible oil is to the Toxicity Determination of prodenia litura for embodiment 2 20% compounded pesticides of the present invention
Above-mentioned missible oil is diluted with water to concentration gradient to be measured, and experimental technique is with embodiment 1.Experimental result sees Table 2, and its co-toxicity coefficient reaches 179.52, has obvious synergistic effect.
Table 2 destruxins, the agent of beta-cypermethrin list and emulsifiable concentrate of the present invention are to the toxicity test result of prodenia litura 3 instar larvaes
Claims (5)
1, a kind of destruxins and efficient cypermethrin compounded desinsection host, the mass ratio that it is characterized in that described destruxins and beta-cypermethrin is 7～1: 3～9.
2, according to the desinsection host of described destruxins of claim 1 and chlorpyrifos mixture, the mass ratio that it is characterized in that described destruxins and beta-cypermethrin is 3～4: 7～6.
3, according to the desinsection host of claim 1 or 2 described destruxinses and chlorpyrifos mixture, the mass ratio that it is characterized in that described destruxins and beta-cypermethrin is 7: 3,5: 5,4: 6,3: 3,3: 7 or 1: 9.
4,, it is characterized in that described destruxins is any one or the two or more mixture that mixes with any ratio among destruxins A, destruxins B or the destruxins E according to claim 1 or 2 described destruxinses and efficient cypermethrin compounded desinsection host.
5, a kind of insecticide comprises host and assistant agent, it is characterized in that by mass percentage, host accounts for 20%, and assistant agent accounts for 80%; Described host is that destruxins is the mixture that mixes at 3: 7 according to mass ratio with beta-cypermethrin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102199989A CN101433215B (en) | 2008-12-15 | 2008-12-15 | Compositional main pesticide and pesticide containing destruxins of Metarhizium anisopliae and high-efficient cypermethrin |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008102199989A CN101433215B (en) | 2008-12-15 | 2008-12-15 | Compositional main pesticide and pesticide containing destruxins of Metarhizium anisopliae and high-efficient cypermethrin |
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| CN101433215A true CN101433215A (en) | 2009-05-20 |
| CN101433215B CN101433215B (en) | 2011-04-27 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823625A (en) * | 2012-09-14 | 2012-12-19 | 广东省昆虫研究所 | Insecticidal composition containing metarhizium anisopliae and tefluthrin |
| CN106614696A (en) * | 2017-02-15 | 2017-05-10 | 海南省植物保护总站（海南省农药检定所） | Insecticidal composition containing bifenthrin and application of insecticidal composition |
| CN108294045A (en) * | 2018-03-15 | 2018-07-20 | 重庆中邦药业（集团）有限公司 | One vegetative insecticide composition, preparation and its application |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100486440C (en) * | 2006-12-26 | 2009-05-13 | 华南农业大学 | Compounding insecticide containing destruxin and admire |
| CN100477920C (en) * | 2006-12-26 | 2009-04-15 | 华南农业大学 | Compounding insecticide containing destruxin and abamectin |
-
2008
- 2008-12-15 CN CN2008102199989A patent/CN101433215B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823625A (en) * | 2012-09-14 | 2012-12-19 | 广东省昆虫研究所 | Insecticidal composition containing metarhizium anisopliae and tefluthrin |
| CN106614696A (en) * | 2017-02-15 | 2017-05-10 | 海南省植物保护总站（海南省农药检定所） | Insecticidal composition containing bifenthrin and application of insecticidal composition |
| CN106614696B (en) * | 2017-02-15 | 2019-09-13 | 海南省植物保护总站（海南省农药检定所） | A kind of Pesticidal combination containing Biphenthrin and its application |
| CN108294045A (en) * | 2018-03-15 | 2018-07-20 | 重庆中邦药业（集团）有限公司 | One vegetative insecticide composition, preparation and its application |
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| CN101433215B (en) | 2011-04-27 |
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