CN101904270A - High-yield bagged material culture method of tea tree mushrooms - Google Patents
High-yield bagged material culture method of tea tree mushrooms Download PDFInfo
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- CN101904270A CN101904270A CN 201010224189 CN201010224189A CN101904270A CN 101904270 A CN101904270 A CN 101904270A CN 201010224189 CN201010224189 CN 201010224189 CN 201010224189 A CN201010224189 A CN 201010224189A CN 101904270 A CN101904270 A CN 101904270A
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Abstract
The invention discloses a high-yield bagged material culture method of tea tree mushrooms, comprising the following steps of bagged material making, inoculation, mycelium culture and fruiting body culture. Bagged material has the advantages of scientific material matching and reasonable ratio and can supply essential nutrition to myceliums and fruiting bodies. The technological conditions of temperature, humidity and the like are controlled strictly in the culture process to accelerate mycelium growth and fruiting body primordium differentiation; thus, the invention has the advantages of good fruiting body development, high biological transformation rate, little misshapen mushroom production, uniform individual fruiting body, high quality, long mushroom production period, high yield, little pollution and low cost and further enhances the utilization ratio of cultural materials.
Description
Technical field
The present invention relates to the arviculture field, be specifically related to a kind of Bag Material high-yield cultivating method of tea tree mushroom.
Background technology
Tea firewood mushroom also claims tea tree mushroom, Agroeybe cylindracea mycelia, Liu Songrong, Liu Huan bacterium etc., is subordinate to excrement rust umbrella section, and how wild fruit body summer, autumn be in the withered position of tea oil tree, is Dan Sheng, twin or from life.Bacteria cover diameter 2-8 centimetre, the first protruding back of giving birth to is open and flat, and the brown limit is lighter, smooth surface; The bacterial context look white, plump, thick 3-6 centimetre, and in the stem true length 13-8 centimetre, thick 3-12 millimeter, there is striped, light brown on middle real surface.
Tea firewood mushroom delicious flavour, tender and crisp good to eat, and contain 18 seed amino acids and multi mineral prime element, traditional Chinese medicine is thought, the flat temperature of tea firewood mushroom property, effect such as clearing damp, diuresis strengthening the spleen and stomach are arranged, make eye bright is one of a kind of rare edible mushroom.
Tea firewood mushroom to lignin, cellulose-decomposing ability a little less than, not long because of the tea tree mushroom cultivation history, production management lacks standard, and this mycelium and fruit body contain one almond flavor simultaneously, damage by disease and insect is more, still has the pollution rate height in cultivation, yields poorly, problem such as poor quality.Tea tree mushroom is cultivated fruiting in spring, two seasons of autumn usually, but cannot not go out intactly mushroom at this season, needs just can go out mushroom through summer, the processing of surviving the winter more when temperature suits.Cultivating in bag is traditional mushroom cultivation method, though with short production cycle, time-consuming taking a lot of work, efficiency is low, and the fruiting phase is short.The cultivation that is used for tea tree mushroom often cause bacterium bag nutrient loss big, yield poorly down, the availability of cultivating facility, resource is low.
Be necessary to overcome above-mentioned defective, a kind of Bag Material high-yield cultivating method of tea tree mushroom is provided, adopt this method cultivate agrocybe mushroom, its fruiting phase is long, biological transformation ratio is high, mycelia survival rate height, output height, pollute less, cost is low, the Stropharia rugoso-annulata fruit body that cultivates is individual evenly, quality better.
Summary of the invention
In view of this, the Bag Material high-yield cultivating method that the purpose of this invention is to provide a kind of tea tree mushroom, adopt this method cultivate agrocybe mushroom, its fruiting phase is long, biological transformation ratio is high, mycelia survival rate height, output height, pollute less, cost is low, the Stropharia rugoso-annulata fruit body that cultivates is individual evenly, quality better.
The Bag Material high-yield cultivating method of tea tree mushroom of the present invention may further comprise the steps:
A. make cultivate material: get hardwood sawdust 74, rice bran 14, corn flour 6, dregs of beans 5 and land plaster 1 by weight as siccative, being mixed and made into pH value with water again is that 5.0-6.0, moisture mass fraction are the cultivate material of 65%-75%;
B. make Bag Material: the amount of trying to please is 700-800cm
3Polypropylene Bag cultivate material is carried out packing, the charge weight of cultivate material is counted 500-600g by siccative in each Polypropylene Bag, Bag Material;
C. inoculation: Bag Material in 98-100 ℃ of normal-pressure sterilization 15-16 hour, is moved in the aseptic inoculation chamber more while hot and is cooled to 25-30 ℃, inoculate then;
D. cultural hypha: postvaccinal Bag Material is carried out cultural hypha in 23-28 ℃, the gnotobasis of 60-70% humidity;
E. fruit body is cultivated: after treating that mycelia is full of Polypropylene Bag, the sack of Polypropylene Bag opened wide and under 85-95% humidity, 18-24 ℃ condition enrichment culture 10-15 days, the fruit body of gathering then promptly.
Further, the vaccination ways among the step c is: adopt the dibbling mode to insert original seed in bacterium bag two ends, every end inoculum concentration is 20 grams, 2 cun of the line-spacings of dibbling, 2 cun of spacing in the rows;
Further, among the step e, fruit body is incubated in the cultivation house carries out, keeping humidity in the cultivation house during the cultivation is that 80-90%, temperature are 20-24 ℃, keep between air in the cultivation house and the atmosphere with the convection current of 0.5-1.5m/s speed, and replaces illumination by 600-700lx light intensity irradiation 8-10 hour, dark 10 hours frequency and gather until fruit body;
Further, cut off the water bacteria 5-10 days after every damp mushroom is gathered, the operation of repeating step e continues to be cultured to gathering of next damp mushroom again, repeats the 2-3 tide of gathering altogether.
The invention has the beneficial effects as follows: the Bag Material high-yield cultivating method of tea tree mushroom of the present invention, comprise the Bag Material making, inoculation, cultural hypha and fruit body are cultivated several big steps, Bag Material materials science, reasonable mixture ratio, the necessary nutrition of mycelia and sporophore growth can be provided, strict control temperature in the cultivation process, process conditions such as humidity, make the differentiation of mycelial growth and fruit body primordium rapidly, fruit body development is good, the biological transformation ratio height, misshapen mushroom generates few, and fruit body is evenly individual, quality better, further improve the availability of cultivate material, make the fruiting phase long, the output height, pollution is few and cost is low.
Other advantages of the present invention, target and feature will be set forth to a certain extent in the following description, and to a certain extent, based on being conspicuous to those skilled in the art, perhaps can obtain instruction from the practice of the present invention to investigating hereinafter.Target of the present invention and other advantages can realize and obtain by following specification and claims.
Embodiment
The present invention will be further described below with reference to embodiment.
Embodiment one:
The Bag Material high-yield cultivating method of the tea tree mushroom of present embodiment may further comprise the steps:
A. make cultivate material: get willow wood chip 74, rice bran 14, corn flour 6, dregs of beans 5 and land plaster 1 by weight as siccative, be mixed and made into water that pH value is 5.0 again, moisture mass fraction is 65% cultivate material;
B. make Bag Material: the amount of trying to please is 700-800cm
3Polypropylene Bag cultivate material is carried out packing, the charge weight of cultivate material is counted 500g by siccative in each Polypropylene Bag, Bag Material;
C. inoculation: with Bag Material in 98-100 ℃ of normal-pressure sterilization 15 hours (sterilising temp at 15-16 hour all can) herein, move in the aseptic inoculation chamber more while hot and be cooled to 25 ℃, adopt the dibbling mode to insert original seed then in bacterium bag two ends, every end inoculum concentration is 20 grams, 2 cun of the line-spacings of dibbling, 2 cun of spacing in the rows;
D. cultural hypha: postvaccinal Bag Material is carried out cultural hypha in 23-25 ℃, the gnotobasis of 60-62% humidity;
E. fruit body is cultivated: after treating that mycelia is covered with Polypropylene Bag, the sack of Polypropylene Bag opened wide and place under 85-88% humidity, the 18-20 ℃ condition reproduction to cultivate 10 days, keep the culture environment ventilation and have scattered light, the fruit body of gathering then gets the first menses mushroom, output is average every bag of 547g, and misshapen mushroom output accounts for 1% of first menses mushroom.
As seen observation post gets fruit body: fruit body is light brown, smooth surface, and the bacterial context look white is, plump; Wherein the bacteria cover diameter of 95% fruit body is between 3.5-4.5 centimetre, cap thickness between the 4.5-5.5 millimeter, stem length between 10-12 centimetre, the stem diameter is between 0.5-1 centimetre.
Embodiment two:
Repeat the operating procedure of embodiment one, difference is:
Among the step a, the pH value of cultivate material is 6.0, moisture mass fraction is 75%; Among the step b, the charge weight of cultivate material is counted 600g by siccative in each Polypropylene Bag; Among the step c, the cooling inoculation temperature is below 30 ℃; In the steps d, the temperature of gnotobasis is that 26-28 ℃, humidity are 68-70%; Among the step e, the fruit body culture environment is humidity 92-95%, temperature 22-24 ℃, and the reproduction incubation time is 15-25 days.
Described first menses mushroom output is average every bag of 556g, and misshapen mushroom output accounts for 1.2% of first menses mushroom.
As seen observation post gets fruit body: as seen observation post gets fruit body: fruit body is light brown, smooth surface, and the bacterial context look white is, plump; Wherein the bacteria cover diameter of 95% fruit body is between 3.5-4.5 centimetre, thickness between the 4.5-5.5 millimeter, stem length between 10-12 centimetre, the stem diameter is between 0.5-1 centimetre.
Embodiment three
Repeat the operating procedure of embodiment one, difference is:
Among the step a, the pH value of cultivate material is 5.0, moisture mass fraction is 75%; Among the step b, the charge weight of cultivate material is counted 500g by siccative in each Polypropylene Bag; Among the step c, the cooling inoculation temperature is 30 ℃; In the steps d, the temperature of gnotobasis is that 23-25 ℃, humidity are 68-70%; Among the step e, the fruit body culture environment is humidity 85-88%, temperature 22-24 ℃, and the enrichment culture time is 10 days.
Described first menses mushroom output is average every bag of 568g, and misshapen mushroom output accounts for 0.9% of first menses mushroom.
As seen observation post gets fruit body: fruit body is light brown, smooth surface, and the bacterial context look white is, plump; Wherein the bacteria cover diameter of 96% fruit body is between 4-5 centimetre, cap thickness between the 5-6 millimeter, stem length at 10-12 centimetre, stem diameter between 0.6-1 centimetre.
Embodiment four
Repeat the operating procedure of embodiment one, difference is:
Among the step a, the pH value of cultivate material is 6.0, moisture mass fraction is 65%; Among the step b, the charge weight of cultivate material is counted 600g by siccative in each Polypropylene Bag; Among the step c, the cooling inoculation temperature is 20 ℃; In the steps d, the temperature of gnotobasis is that 26-28 ℃, humidity are 60-62%; Among the step e, the fruit body culture environment is humidity 93-95%, temperature 20-22 ℃, and the enrichment culture time is 15 days.
Described first menses mushroom output is average every bag of 554g, and misshapen mushroom output accounts for 1.3% of first menses mushroom.
As seen observation post gets fruit body: fruit body is light brown, smooth surface, and the bacterial context look white is, plump; Wherein the bacteria cover diameter of 95% fruit body is between 4-5 centimetre, cap thickness between the 5-5.5 millimeter, stem length between 8-12 centimetre, the stem diameter is between 0.6-1 centimetre.
By embodiment 1 to embodiment 4 data show, adopt the inventive method cultivate agrocybe mushroom, the output of its first menses mushroom (adopts the cultivate material situation cultivation method cultivation routinely of identical weight compared to conventional cultivation method gained output, output is generally about every bag of 470-490g) all higher, and the misshapen mushroom yield is low, the individual uniformity of fruit body, quality better.
Implement five
Repeat the operating procedure of embodiment three, difference is: among the step e, fruit body is incubated in the cultivation house carries out, keep in the cultivation house humidity 92-95%, temperature 18-24 ℃ during the cultivation, keep between air in the cultivation house and the atmosphere, and replace illumination by 600lx light intensity irradiation 8 hours, dark 10 hours frequency and gather until fruit body with the convection current of 0.5-1.0m/s speed.
Described first menses mushroom output is average every bag of 562g, and misshapen mushroom output accounts for 0.8% of first menses mushroom.
As seen observation post gets fruit body: fruit body is light brown, smooth surface, and the bacterial context look white is, plump; Wherein the bacteria cover diameter of 96% fruit body is between 4-5 centimetre, cap thickness between the 5-6 millimeter, stem length at 9-12 centimetre, stem diameter between 0.7-1 centimetre.
Embodiment six
Repeat the operating procedure of embodiment three, difference is: among the step e, fruit body is incubated in the cultivation house carries out, keep in the cultivation house humidity 83-85%, temperature 21-22 ℃ during the cultivation, keep between air in the cultivation house and the atmosphere, and replace illumination by 700lx light intensity irradiation 10 hours, dark 10 hours frequency and gather until fruit body with the convection current of 1.0-1.5m/s speed.
Described first menses mushroom output is average every bag of 549g, and misshapen mushroom output accounts for 0.5% of first menses mushroom.
As seen observation post gets fruit body: fruit body is light brown, smooth surface, and the bacterial context look white is, plump; Wherein the bacteria cover diameter of 96% fruit body is between 4-5 centimetre, cap thickness between the 5-6 millimeter, stem length at 10-12 centimetre, stem diameter between 0.6-1 centimetre.
Embodiment seven
Repeat the operating procedure of embodiment three, difference is: among the step e, fruit body is incubated in the cultivation house carries out, keep interior humidity 83-85%, temperature 22-24 ℃ of cultivation house during the cultivation, keep between interior air of cultivation house and the atmosphere with the convection current of 1.0-1.5m/s speed, and replace illumination by 600lx light intensity irradiation 10 hours, dark 10 hours frequency and gather until the first menses mushroom, cut off the water then bacteria 5 days, the operation of repeating step e is gathered until two damp mushrooms.
Described first menses mushroom output is average every bag of 551g, and two damp mushroom output are average every bag of 498g, and misshapen mushroom output accounts for 0.9% of first menses mushroom.
As seen observation post gets fruit body: fruit body is light brown, smooth surface, and the bacterial context look white is, plump; Wherein the bacteria cover diameter of 92% fruit body is between 4-5 centimetre, thickness between the 5-5.5 millimeter, stem length between 10-12 centimetre, the stem diameter is between 0.6-1 centimetre.
Embodiment eight
Repeat the operating procedure of embodiment seven, difference is: among the step e, fruit body is incubated in the cultivation house carries out, keep interior humidity 88-90%, temperature 20-22 ℃ of cultivation house during the cultivation, keep between interior air of cultivation house and the atmosphere with the convection current of 1.0-1.5m/s speed, and replace illumination by 700lx light intensity irradiation 8 hours, dark 10 hours frequency and gather until the first menses mushroom, cut off the water then bacteria 10 days, the operation of repeating step e is gathered until three damp mushrooms.
Described first menses mushroom output is average every bag of 560g, and two damp mushroom output are average every bag of 501g, and three damp mushroom output are average every bag of 421g, and misshapen mushroom output accounts for 1.0% of two damp mushrooms, three damp mushrooms.
As seen observation post gets fruit body: fruit body is light brown, smooth surface, and the bacterial context look white is, plump; Wherein the bacteria cover diameter of 93% fruit body is between 4-5 centimetre, thickness between the 5-6 millimeter, stem length at 10-11 centimetre, stem diameter between 0.6-1 centimetre.
By embodiment five to embodiment eight data as can be known, fruit body is cultivated in cultivating the room and is carried out, and will cultivate that environmental condition can (be that misshapen mushroom is few in guarantee mushroom product quality by conditional parameter setting of the present invention in the room in, individual even, the quality better of fruit body) time, improve the single moushroom yield, further improve raw material availability, make under the cultivating in bag, the fruiting phase is long, fruiting strength foot, it is many to produce mushroom tide number, significantly reduces cost and increases economic benefit.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement technical scheme of the present invention, and not breaking away from the aim and the scope of the technical program, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (4)
1. the Bag Material high-yield cultivating method of tea tree mushroom is characterized in that: may further comprise the steps:
A. make cultivate material: get hardwood sawdust 74, rice bran 14, corn flour 6, dregs of beans 5 and land plaster 1 by weight as siccative, being mixed and made into pH value with water again is that 5.0-6.0, moisture mass fraction are the cultivate material of 65%-75%;
B. make Bag Material: the amount of trying to please is 700-800cm
3Polypropylene Bag cultivate material is carried out packing, the charge weight of cultivate material is counted 500-600g by siccative in each Polypropylene Bag, Bag Material;
C. inoculation: Bag Material in 98-100 ℃ of normal-pressure sterilization 15-16 hour, is moved in the aseptic inoculation chamber more while hot and is cooled to 25-30 ℃, inoculate then;
D. cultural hypha: postvaccinal Bag Material is carried out cultural hypha in 23-28 ℃, the gnotobasis of 60-70% humidity;
E. fruit body is cultivated: after treating that mycelia is full of Polypropylene Bag, the sack of Polypropylene Bag opened wide and under 85-95% humidity, 18-24 ℃ condition enrichment culture 10-15 days, the fruit body of gathering then promptly.
2. the Bag Material high-yield cultivating method of tea tree mushroom according to claim 1, it is characterized in that: the vaccination ways among the step c is: adopt the dibbling mode to insert original seed in bacterium bag two ends, every end inoculum concentration is 20 grams, 2 cun of the line-spacings of dibbling, 2 cun of spacing in the rows.
3. the Bag Material high-yield cultivating method of tea tree mushroom according to claim 1, it is characterized in that: among the step e, fruit body is incubated in the cultivation house carries out, keeping humidity in the cultivation house during the cultivation is that 80-90%, temperature are 20-24 ℃, keep between air in the cultivation house and the atmosphere with the convection current of 0.5-1.5m/s speed, and replaces illumination by 600-700lx light intensity irradiation 8-10 hour, dark 10 hours frequency and gather until fruit body.
4. according to the Bag Material high-yield cultivating method of the described tea tree mushroom of the arbitrary claim of claim 1 to 3, it is characterized in that: cut off the water after every damp mushroom is gathered bacteria 5-10 days, the operation of repeating step e continue to be cultivated until the gathering of next damp mushroom again, and 2-3 tide altogether repeats to gather.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103304287A (en) * | 2013-06-28 | 2013-09-18 | 广西巴马原生长寿食品有限公司 | Culture medium and cultivation method of agrocybe cylindracea |
| CN103304341A (en) * | 2013-07-16 | 2013-09-18 | 王龙 | Agrocybe cylindracea culture medium and preparation method thereof |
| CN103333022A (en) * | 2013-07-04 | 2013-10-02 | 邬金飞 | Cultivation material compatibility of medicines for agrocybe cylindracea and manufacture method of cultivation material |
| CN103583226A (en) * | 2012-08-14 | 2014-02-19 | 玉林市微生物研究所 | Good quality and high yield cultivation method for tea tree mushroom |
| CN103975763A (en) * | 2014-05-14 | 2014-08-13 | 河北沃达生物技术有限公司 | Agrocybe cylindracea cultivation method |
| CN104472212A (en) * | 2014-11-25 | 2015-04-01 | 曾令钢 | Factory-like bagging cultivation method for agrocybe cylindracea |
| CN104663247A (en) * | 2015-03-12 | 2015-06-03 | 象州县科学技术局 | Method for utilizing mulberry branch powder to cultivate agrocybe cylindracea |
| CN104770215A (en) * | 2015-03-22 | 2015-07-15 | 邬金飞 | Agrocybe cylindracea cultivation method |
| CN104770214A (en) * | 2015-03-22 | 2015-07-15 | 邬金飞 | Agrocybe cylindracea cultivation method |
| CN107409757A (en) * | 2017-08-15 | 2017-12-01 | 西南林业大学 | The white ginseng bacterium bag material and cultural method formed with Eupatorium adenophorum and organic material |
| CN111328631A (en) * | 2020-04-14 | 2020-06-26 | 重庆市城口县松坤菌草科技开发有限责任公司 | Flower mushroom cultivation method suitable for high-altitude areas |
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| CN1145160A (en) * | 1995-09-14 | 1997-03-19 | 谢远泰 | Tech. for cultivating tea tree mushroom in fields |
Non-Patent Citations (1)
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| CN103583226B (en) * | 2012-08-14 | 2016-08-03 | 玉林市微生物研究所 | A kind of Agrocybe aegerita (Brig.) Sing. cultivating superior high-yield method |
| CN103583226A (en) * | 2012-08-14 | 2014-02-19 | 玉林市微生物研究所 | Good quality and high yield cultivation method for tea tree mushroom |
| CN103304287A (en) * | 2013-06-28 | 2013-09-18 | 广西巴马原生长寿食品有限公司 | Culture medium and cultivation method of agrocybe cylindracea |
| CN103333022A (en) * | 2013-07-04 | 2013-10-02 | 邬金飞 | Cultivation material compatibility of medicines for agrocybe cylindracea and manufacture method of cultivation material |
| CN103304341A (en) * | 2013-07-16 | 2013-09-18 | 王龙 | Agrocybe cylindracea culture medium and preparation method thereof |
| CN103304341B (en) * | 2013-07-16 | 2015-06-10 | 王龙 | Agrocybe cylindracea culture medium and preparation method thereof |
| CN103975763A (en) * | 2014-05-14 | 2014-08-13 | 河北沃达生物技术有限公司 | Agrocybe cylindracea cultivation method |
| CN104472212A (en) * | 2014-11-25 | 2015-04-01 | 曾令钢 | Factory-like bagging cultivation method for agrocybe cylindracea |
| CN104472212B (en) * | 2014-11-25 | 2016-08-24 | 曾令钢 | A kind of agrocyb eaegerita batch production bag cultivating method |
| CN104663247A (en) * | 2015-03-12 | 2015-06-03 | 象州县科学技术局 | Method for utilizing mulberry branch powder to cultivate agrocybe cylindracea |
| CN104770214A (en) * | 2015-03-22 | 2015-07-15 | 邬金飞 | Agrocybe cylindracea cultivation method |
| CN104770215A (en) * | 2015-03-22 | 2015-07-15 | 邬金飞 | Agrocybe cylindracea cultivation method |
| CN107409757A (en) * | 2017-08-15 | 2017-12-01 | 西南林业大学 | The white ginseng bacterium bag material and cultural method formed with Eupatorium adenophorum and organic material |
| CN111328631A (en) * | 2020-04-14 | 2020-06-26 | 重庆市城口县松坤菌草科技开发有限责任公司 | Flower mushroom cultivation method suitable for high-altitude areas |
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| CN101904270B (en) | 2012-02-08 |
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