CN101868425B - 通过电解控制pH梯度以及它们在等电聚焦中的用途 - Google Patents

通过电解控制pH梯度以及它们在等电聚焦中的用途 Download PDF

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Publication number
CN101868425B
CN101868425B CN2008801146906A CN200880114690A CN101868425B CN 101868425 B CN101868425 B CN 101868425B CN 2008801146906 A CN2008801146906 A CN 2008801146906A CN 200880114690 A CN200880114690 A CN 200880114690A CN 101868425 B CN101868425 B CN 101868425B
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volume
proton concentration
environment
proton
electrode
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Expired - Fee Related
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CN101868425A (zh
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U·西万
E·布罗德
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Technion Research and Development Foundation Ltd
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Technion Research and Development Foundation Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
    • C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
    • C02F1/46104—Devices therefor; Their operating or servicing
    • C02F1/46109—Electrodes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
    • C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
    • C02F1/46104—Devices therefor; Their operating or servicing
    • C02F1/4618—Devices therefor; Their operating or servicing for producing "ionised" acidic or basic water
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
    • C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
    • C02F1/46104—Devices therefor; Their operating or servicing
    • C02F1/46109—Electrodes
    • C02F2001/46152—Electrodes characterised by the shape or form
    • C02F2001/46157—Perforated or foraminous electrodes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2201/00—Apparatus for treatment of water, waste water or sewage
    • C02F2201/46—Apparatus for electrochemical processes
    • C02F2201/461—Electrolysis apparatus
    • C02F2201/46105—Details relating to the electrolytic devices
    • C02F2201/46115—Electrolytic cell with membranes or diaphragms
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2201/00—Apparatus for treatment of water, waste water or sewage
    • C02F2201/46—Apparatus for electrochemical processes
    • C02F2201/461—Electrolysis apparatus
    • C02F2201/46105—Details relating to the electrolytic devices
    • C02F2201/4612—Controlling or monitoring
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2201/00—Apparatus for treatment of water, waste water or sewage
    • C02F2201/46—Apparatus for electrochemical processes
    • C02F2201/461—Electrolysis apparatus
    • C02F2201/46105—Details relating to the electrolytic devices
    • C02F2201/4612—Controlling or monitoring
    • C02F2201/46125—Electrical variables
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2201/00—Apparatus for treatment of water, waste water or sewage
    • C02F2201/46—Apparatus for electrochemical processes
    • C02F2201/461—Electrolysis apparatus
    • C02F2201/46105—Details relating to the electrolytic devices
    • C02F2201/4612—Controlling or monitoring
    • C02F2201/46125—Electrical variables
    • C02F2201/4614—Current
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/06—Controlling or monitoring parameters in water treatment pH

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Environmental & Geological Engineering (AREA)
  • Engineering & Computer Science (AREA)
  • Hydrology & Water Resources (AREA)
  • Water Supply & Treatment (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Electrolytic Production Of Non-Metals, Compounds, Apparatuses Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN2008801146906A 2007-08-27 2008-08-26 通过电解控制pH梯度以及它们在等电聚焦中的用途 Expired - Fee Related CN101868425B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US93569807P 2007-08-27 2007-08-27
US60/935698 2007-08-27
US60/935,698 2007-08-27
US3925708P 2008-03-25 2008-03-25
US61/039257 2008-03-25
US61/039,257 2008-03-25
PCT/IL2008/001159 WO2009027970A2 (en) 2007-08-27 2008-08-26 Ph gradients controlled by electrolysis, and their use in isoelectric focusing

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201210450797.6A Division CN103134843B (zh) 2007-08-27 2008-08-26 通过电解控制ph梯度以及它们在等电聚焦中的用途

Publications (2)

Publication Number Publication Date
CN101868425A CN101868425A (zh) 2010-10-20
CN101868425B true CN101868425B (zh) 2013-01-02

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CN2008801146906A Expired - Fee Related CN101868425B (zh) 2007-08-27 2008-08-26 通过电解控制pH梯度以及它们在等电聚焦中的用途
CN201210450797.6A Expired - Fee Related CN103134843B (zh) 2007-08-27 2008-08-26 通过电解控制ph梯度以及它们在等电聚焦中的用途

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US (2) US9274082B2 (enExample)
EP (2) EP2559666B1 (enExample)
JP (1) JP5702142B2 (enExample)
KR (1) KR20100053672A (enExample)
CN (2) CN101868425B (enExample)
AU (1) AU2008293381B2 (enExample)
CA (1) CA2697649C (enExample)
WO (1) WO2009027970A2 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2559666B1 (en) 2007-08-27 2014-07-23 Technion Research & Development Foundation Ltd. pH gradients controlled by electrolysis, and their use in isoelectric focusing
US10107782B2 (en) * 2008-01-25 2018-10-23 ProteinSimple Method to perform limited two dimensional separation of proteins and other biologicals
JP5550729B2 (ja) * 2009-08-18 2014-07-16 テクニオン リサーチ アンド ディベロップメント ファウンデーション リミテッド プロトン濃度トポグラフィーならびにその生成方法および生成デバイス
WO2011021195A1 (en) 2009-08-18 2011-02-24 Technion Research & Development Foundation Ltd. Methods and devices of separating molecular analytes
JP5950429B2 (ja) * 2011-05-13 2016-07-13 シャープ株式会社 電気泳動方法、及び電気泳動装置
US9234875B2 (en) 2011-11-04 2016-01-12 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
EP2773743B1 (en) 2011-11-04 2019-05-22 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of ph
WO2013067529A1 (en) * 2011-11-04 2013-05-10 Bio-Rad Laboratories, Inc. PROTEIN FRACTIONATION BASED ON pI
CN102565171A (zh) * 2012-01-05 2012-07-11 厦门大学 一种用于等电聚焦分离的微流控芯片
EP2814597A4 (en) 2012-02-15 2015-10-28 Bio Rad Laboratories ELECTRONIC CONTROL OF PH-VALUE AND ION STRENGTH
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
CN104034789A (zh) * 2013-04-02 2014-09-10 上海交通大学 蛋白质等电聚焦电泳的装置
CN106260992B (zh) * 2015-06-09 2020-02-18 海南椰国食品有限公司 一种含有酸性椰果和/或碱性椰果的食品
CN106267304B (zh) * 2015-06-09 2019-10-18 海南光宇生物科技有限公司 具有酸碱性的生物纤维素凝胶膜及由其制备的医用敷料
EP3653121A1 (en) * 2018-11-14 2020-05-20 Koninklijke Philips N.V. Sensor unit, body fluid monitoring device and method for detecting an analyte
EP4202431B1 (en) * 2021-12-23 2025-11-12 Imec VZW Electric field gradient focusing device
CN119470588A (zh) * 2024-11-18 2025-02-18 中国科学院空天信息创新研究院 集成式多种重金属检测微传感器

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3682502D1 (de) * 1985-08-21 1991-12-19 Biotope Inc Verfahren und geraete zur trennung, vermischung und bestimmung von komponenten in spezifischen bindungstests.
US4670119A (en) * 1985-10-25 1987-06-02 Hurd Stanley M Isoelectric focusing device and process
US4900414A (en) * 1988-08-19 1990-02-13 Drug Delivery Systems Inc. Commercial separation system and method using electrokinetic techniques
US5160594A (en) 1989-03-08 1992-11-03 Board Of Regents Of The University Of Texas System Apparatus and methods for isoelectric focusing of amphoteric substances incorporating ion selective membranes in electrode chambers
DE3929137C1 (enExample) * 1989-09-01 1991-02-28 Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung Ev, 8000 Muenchen, De
US5110434A (en) * 1990-12-20 1992-05-05 Bio-Rad Laboratories, Inc. Use of zwitterions to mobilize isoelectrically focused ampholyte zones
US5646001A (en) * 1991-03-25 1997-07-08 Immunivest Corporation Affinity-binding separation and release of one or more selected subset of biological entities from a mixed population thereof
GB2275974B (en) 1993-03-10 1996-01-10 Curty Payen Sa Improvements in and relating to gaskets
US6129828A (en) * 1996-09-06 2000-10-10 Nanogen, Inc. Apparatus and methods for active biological sample preparation
JPH0933479A (ja) * 1995-07-18 1997-02-07 Mizu Kk センサカバー及びこれを用いた電解水生成装置
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
WO1999063332A1 (en) * 1998-06-05 1999-12-09 Sarnoff Corporation Apparatus for separating molecules
JP3113645B2 (ja) * 1999-03-01 2000-12-04 ファースト・オーシャン株式会社 電解水製造法
WO2000073780A1 (en) * 1999-05-28 2000-12-07 Proteologics, Inc. Methods and apparatus for nonlinear mobility electrophoresis separation
DE10047088C2 (de) 2000-09-21 2002-10-17 Gerhard Weber Medium für analytische und präparative Elektrophorese
JP2002265494A (ja) 2001-03-06 2002-09-18 Tokuyama Corp 混合物から高純度化された目的物を製造する方法
EP1410029B1 (en) 2001-07-16 2007-09-12 Protein Forest, Inc. Arrays of buffers for analysing biomolecules by their isoelectric point
GB0121189D0 (en) * 2001-08-31 2001-10-24 Diagnoswiss Sa Apparatus and method for separating an analyte
SE0203773D0 (sv) * 2002-12-19 2002-12-19 Capture Device Ab Method and device for capturing charged molecules traveling in a flow stream
US7150813B2 (en) * 2003-06-12 2006-12-19 Palo Alto Research Center Incorporated Isoelectric focusing (IEF) of proteins with sequential and oppositely directed traveling waves in gel electrophoresis
WO2005021841A2 (en) 2003-09-03 2005-03-10 Bioforms Methods and apparatus for rapid crystallization of biomolecules
JP2006213932A (ja) 2004-05-07 2006-08-17 Honda Motor Co Ltd 電解水生成用電極
CN1258828C (zh) 2004-02-13 2006-06-07 清华大学 一种质子交换膜燃料电池多元催化剂的制备方法
US7238272B2 (en) * 2004-02-27 2007-07-03 Yoichi Sano Production of electrolytic water
WO2006014680A1 (en) * 2004-07-19 2006-02-09 Cell Biosciences, Inc. Methods and devices for analyte detection
US7749370B2 (en) * 2005-02-03 2010-07-06 Osao Sumita Manufacturing method of oxidative water to be employed for sterilization
JP5129442B2 (ja) 2005-02-08 2013-01-30 エア・ウォーター株式会社 液体の帯電物質の濃度調節装置
JP4856530B2 (ja) * 2005-12-21 2012-01-18 ミドリ安全株式会社 電解水の有効塩素濃度調節方法、電解水のｐＨ調節方法および電解水生成装置。
ATE486046T1 (de) 2006-02-17 2010-11-15 Actides Gmbh Verfahren zur herstellung eines desinfektionsmittels durch elektrochemische aktivierung (eca) von wasser
JP3163188U (ja) 2007-03-13 2010-10-07 シーメンス ウォーター テクノロジース コーポレイション 酸及び塩基生成のための装置及び方法
WO2008131328A1 (en) 2007-04-20 2008-10-30 University Of South Carolina Mineral oil free isoelectric focusing apparatus for immobilized ph gradient strips
CN101294930B (zh) 2007-04-27 2013-08-14 杨春 一种整体型固定化pH梯度的制备方法及其应用
US8366899B2 (en) * 2007-06-22 2013-02-05 Massachusetts Institute Of Technology Isoelectric focusing systems and methods
EP2559666B1 (en) 2007-08-27 2014-07-23 Technion Research & Development Foundation Ltd. pH gradients controlled by electrolysis, and their use in isoelectric focusing
JP5550729B2 (ja) 2009-08-18 2014-07-16 テクニオン リサーチ アンド ディベロップメント ファウンデーション リミテッド プロトン濃度トポグラフィーならびにその生成方法および生成デバイス
WO2011021195A1 (en) * 2009-08-18 2011-02-24 Technion Research & Development Foundation Ltd. Methods and devices of separating molecular analytes
WO2013067529A1 (en) * 2011-11-04 2013-05-10 Bio-Rad Laboratories, Inc. PROTEIN FRACTIONATION BASED ON pI

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Publication number Publication date
EP2559666A3 (en) 2013-04-03
US10132776B2 (en) 2018-11-20
US20100307920A1 (en) 2010-12-09
JP2010538255A (ja) 2010-12-09
EP2193100B1 (en) 2014-05-07
AU2008293381B2 (en) 2012-07-19
CA2697649A1 (en) 2009-03-05
EP2559666A2 (en) 2013-02-20
CN103134843A (zh) 2013-06-05
KR20100053672A (ko) 2010-05-20
JP5702142B2 (ja) 2015-04-15
WO2009027970A3 (en) 2009-05-14
AU2008293381A1 (en) 2009-03-05
CN101868425A (zh) 2010-10-20
EP2559666B1 (en) 2014-07-23
US20160178578A1 (en) 2016-06-23
CA2697649C (en) 2016-08-09
WO2009027970A2 (en) 2009-03-05
US9274082B2 (en) 2016-03-01
CN103134843B (zh) 2014-11-12
EP2193100A2 (en) 2010-06-09

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