CN101862351B - Application of active parts of gallnut in preparing anti-ulcerative colitis medicine - Google Patents
Application of active parts of gallnut in preparing anti-ulcerative colitis medicine Download PDFInfo
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Abstract
The invention relates to an application of active parts of gallnut in preparing an anti-ulcerative colitis medicine, wherein the active parts of the gallnut are obtained by water extraction and then organic solvent extraction and mainly comprise a tannin type; and then the active parts are subject to extraction separation through a gel chromatography to obtain a monomeric compound, gallic acid, m-digallic acid, methyl gallate, 1,2,3,6-4-O-galloyl group-Beta-D-glucose and 1,2,3,4,6-5-O-galloyl group-Beta-D-glucose. Clinical outcomes show that the active parts of the gallnut have obvious therapeutic action on ulcerative colitis with the total effective rate up to 91.7%, the short-term cure rate up to 66.7%, effective rate of 25% and inefficiency of 8.3%. Pharmacologic research shows that the active parts of the gallnut can obviously increase SOD content and lower MPO content of an ulcer tissue, and has obvious therapeutic action on rat ulcerative colitis, obvious anti-inflammatory and analgesic action and bacteriostatic action on the common pathogenic bacteria related to ulcerative colitis.
Description
Technical field
The present invention relates to a kind of medicinal application of active parts of gallnut, especially treat the purposes of the medicine of anti-ulcerative colitis effect in preparation.
Background technology
Galla Turcica (Galla Helepensis) is the larva of the insecticide gall wasps Cynips gallae-tinctorice Oliv of gall wasps section, parasitizes on the Fagaceae plant Galla Turcica (Galla Helepensis) tree Quercus infectoria Oliv. sprout insect gall that is produced.Galla Turcica (Galla Helepensis) property is done cold, and bitter and puckery flavor has the effect of astringent or styptic treatment for spontaneous sweating, convergence, dampness, hemostasis, antiinflammatory, is the Uygur medicine medicinal herbs most in use.The Uygur medicine theory thinks that it is directed against the intestinal wall absorbing power that colitis causes and the power that pinches descends, and directly acts on intestinal wall, holds back intestinal, and antiinflammatory antidiarrheal is opened the intestinal resistance, impels ulcer healing.The clinical treatment that is used for ulcerative colitis, evident in efficacy.But relevant active parts of gallnut does not appear in the newspapers as the medicinal usage of preparation anti-ulcerative colitis effect as yet.
(ulcerative colitis UC) claims nonspecific ulcerative colitis again to ulcerative colitis, is a kind of agnogenic chronic rectum and colon inflammatory diseases, is main clinical manifestation with stomachache, diarrhoea, mucus hemafecia, tenesmus.Pathological changes mainly is positioned at the mucous layer of colon, is main with ulcer, involves rectum and far-end colon more, also can spread all over whole colon.Epidemiologic data prompting, and though the sickness rate of ulcerative colitis at home with the trend that increases is year by year abroad all arranged, China's ulcerative colitis prevalence over nearly 10 years than increasing the fifties in last century about 10 times; 20 the center investigation in Europe show that the annual morbidity of ulcerative colitis is 11.2/100000; This disease can betide any age, and is relevant with the morbidity of colon cancer, and the course of disease is long; the lesion degree weight is different; because etiology unknown, and usually performance shows effect and to cure difficulty big repeatedly clinically, is classified as one of modern difficult treatment by World Health Organization (WHO).
Summary of the invention
The objective of the invention is to, the purposes of a kind of active parts of gallnut as preparation treatment anti-ulcerative colitis drugs with function is provided, what this active parts of gallnut adopted that water carries the back organic solvent extraction can obtain active parts of gallnut, mainly comprises the tannin class; Again effective site can be obtained monomeric compound through the gel chromatography extraction separation, comprise gallic acid ,-digallic acid, gallicin, 1,2; 3,6-four-O-galloyl-beta-D-glucose, 1,2; 3,4,6-five-O-galloyl-beta-D-glucose.Show that through clinical effectiveness active parts of gallnut has the obvious treatment effect to ulcerative colitis, total effective rate 91.7%, cure rate 66.7% effectively is 25% in the recent period, invalid is 8.3%.Pharmacological research shows that active parts of gallnut has the obvious treatment effect to the rat ulcer colitis; Can significantly increase SOD content in the chronic ulcer tissue, reduce MPO content, and tangible antiinflammatory, analgesic activity are arranged, the common pathogen relevant to ulcerative colitis has bacteriostasis, and active parts of gallnut is the good medicine of treatment ulcerative colitis.
Active parts of gallnut of the present invention is in the purposes of the medicine of preparation treatment anti-ulcerative colitis, and the method for distilling of active parts of gallnut follows these steps to carry out:
A, get dry Galla Turcica (Galla Helepensis) pulverizing medicinal materials, cross sieve No. 2, take by weighing the 100g medicinal powder, extracting in water 3 times adds 5-15 times of water gaging at every turn, extracts 20-120min;
B, extracting solution is evaporated to 500-1500ml, concentrated solution discards ether layer with the clarification of extracted with diethyl ether to ether layer;
C, with water layer position reuse ethyl acetate extraction, reclaim ethyl acetate, 45-90 ℃ of vacuum drying temperature obtains the active parts of gallnut of 30-60%;
D, the active parts of gallnut that step c is obtained carry out the separation of chemical constituent with the conventional method gel chromatography column, can obtain monomeric compound, comprise gallic acid ,-digallic acid, gallicin, 1; 2,3,6-four-O-galloyl-beta-D-glucose, 1; 2; 3,4,6-five-O-galloyl-beta-D-glucose.
Active parts of gallnut adopts version pharmacopeia appendix X B content of tannin algoscopy in 2000, and the content of measuring tannin is greater than 50%.
The present invention is on the basis of Galla Turcica (Galla Helepensis) clinical treatment ulcerative colitis effectiveness; Galla Turcica (Galla Helepensis) has been carried out than systematic research: (1) is conceived to the safety of Galla halepensis extract; Galla halepensis extract has been carried out general toxicology research, rat has been carried out rectally acute toxicity test, the property test of rectally mucosa irritation and long term toxicity test respectively.Rat rectally The acute toxicity tests shows: the rectum single-dose, and its MTD＞13g/kg is 245 times of the clinical plan dosage of being grown up, and this dosage is not seen drug-induced toxic reaction, and pathological anatomy is not seen the obvious pathological change of Rats Organs and Tissues yet.Rat rectally mucomembranous pungency test and long term toxicity test result show: rat is rectally repeatedly, does not see that drug-induced mucous membrane of rectum is congested, red and swollen phenomenon, and histopathology is not seen the drug-induced pathological change of rat mucous membrane of rectum.Galla halepensis extract does not have obvious irritation.3 various dose administrations of Galla halepensis extract group rat and matched group compare, and the equal no significant difference of each item index is not seen the pathological change relevant with drug effect.Galla halepensis extract is under these 3 various dose conditions, and administration did not have overt toxicity to rat in 120 days.(2) be the therapeutical effect of research Galla Turcica (Galla Helepensis) to rat ulcer colitis (UC) animal model; Adopt TNB and ethanol complex method to prepare rat UC model; After pathological section confirms the modeling success; Treat with Galla halepensis extract, estimate rat colon mucosa injury index (CMDI), detection body weight, the heavy exponential sum immune organ weight of intestinal index variation, detect superoxide dismutase (SOD), myeloperoxidase (MPO) (MPO) activity in the rat colon tissue.Result of the test shows that Galla halepensis extract high dose group animal ulcer is healing fully all, compares with the model group animal, and SOD significantly increases (P＜0.01) in the high and low dose treated animal tissue, and MPO significantly reduces (P＜0.01).Galla halepensis extract has the obvious treatment effect to the rat ulcer colitis.(3) serve as to investigate index with the Galla Turcica (Galla Helepensis) total tannin, extraction process is carried out screening test, Galla Turcica (Galla Helepensis) water is carried the back and is used extracted with diethyl ether as a result; Water layer position reuse ethyl acetate extraction reclaims ethyl acetate, vacuum drying; The dry extract yield is higher than 40%, and total tannin content is greater than 60%.This method of employing enrichment Galla Turcica (Galla Helepensis) total tannin preferably is described.
By conventional method the active parts of gallnut that extracts is processed oral formulations, said preparation is sick as preparation treatment anti-ulcerative colitis.
Active parts of gallnut anti-ulcerative colitis new drug is intended the Galla Turcica (Galla Helepensis) herb resource that utilizes nature abundant, development anti-ulcerative colitis uighur medicine preparation and new drug.Be the preparation that effective site-the Galla Turcica (Galla Helepensis) total tannin is processed that from the Galla Turcica (Galla Helepensis) medical material, extracts, be used to treat ulcerative colitis clinically, clinical efficacy is remarkable.Said preparation will be promoted the use of as the active drug of treatment ulcerative colitis disease, remove slight illness to patients of ulcerative colitis, bring glad tidings.
The specific embodiment
Embodiment 1
A, get dry Galla Turcica (Galla Helepensis) pulverizing medicinal materials, cross sieve No. 2, take by weighing the 100g medicinal powder, extracting in water 3 times adds 5 times of water gagings at every turn, extracts 20min;
B, extracting solution is evaporated to 500ml, concentrated solution discards ether layer with the clarification of extracted with diethyl ether to ether layer;
C, with water layer position reuse ethyl acetate extraction, reclaim ethyl acetate, vacuum drying, 45 ℃ of temperature obtain 30% active parts of gallnut;
D, active parts of gallnut that step c is obtained are with gel chromatography column (model: Sephadex LH-20) carry out the separation of chemical constituent; Carry out gradient elution with water and 20%-100% methanol; The extractum I of 40%-70% meoh eluate and the extractum II of 70%-100% meoh eluate have been obtained; Again the extractum I is used model respectively with II: MCI-GEL CHP-20P gel chromatography column separates, with water and 20%-80% methanol gradient elution; The extractum I has obtained the chemical compound gallic acid ,-digallic acid and gallicin; The extractum II has obtained the extractum III and the extractum IV of 30%-60% meoh eluate; Respectively the extractum III is used model with the extractum IV again: Sephadex LH-20 gel chromatography column separates; Methanol with water and 20%-100% carries out gradient elution, in the meoh eluate of the 60%-100% of extractum III, has obtained chemical compound 1,2; 3,6-four-O-galloyl-beta-D-glucose; In 40%～60% meoh eluate of extractum IV, obtained chemical compound 1,2,3,4,6-five-O-galloyl-beta-D-glucose.
Active parts of gallnut adopts version pharmacopeia appendix X B content of tannin algoscopy in 2000, and the content of measuring tannin is greater than 50%.
Embodiment 2
A, get dry Galla Turcica (Galla Helepensis) pulverizing medicinal materials, cross sieve No. 2, take by weighing the 100g medicinal powder, extracting in water 3 times adds 10 times of water gagings at every turn, extracts 50min;
B, extracting solution is evaporated to 800ml, concentrated solution discards ether layer with the clarification of extracted with diethyl ether to ether layer;
C, with water layer position reuse ethyl acetate extraction, reclaim ethyl acetate, 65 ℃ of vacuum drying temperature obtain 45% active parts of gallnut;
D, active parts of gallnut that step c is obtained are with gel chromatography column (model: Sephadex LH-20) carry out the separation of chemical constituent; Carry out gradient elution with water and 20%-100% methanol; The extractum I of 40%-70% meoh eluate and the extractum II of 70%-100% meoh eluate have been obtained; Again the extractum I is used model respectively with II: MCI-GEL CHP-20P gel chromatography column separates, with water and 20%-80% methanol gradient elution; The extractum I has obtained the chemical compound gallic acid ,-digallic acid and gallicin; The extractum II has obtained the extractum III and the extractum IV of 30%-60% meoh eluate; Respectively the extractum III is used model with the extractum IV again: Sephadex LH-20 gel chromatography column separates; Methanol with water and 20%-100% carries out gradient elution, in the meoh eluate of the 60%-100% of extractum III, has obtained chemical compound 1,2; 3,6-four-O-galloyl-beta-D-glucose; In the meoh eluate of the 40%-60% of extractum IV, obtained chemical compound 1,2,3,4,6-five-O-galloyl-beta-D-glucose.
Active parts of gallnut adopts version pharmacopeia appendix X B content of tannin algoscopy in 2000, and the content of measuring tannin is greater than 50%.
Embodiment 3
A, get dry Galla Turcica (Galla Helepensis) pulverizing medicinal materials, cross sieve No. 2, take by weighing the 100g medicinal powder, extracting in water 3 times adds 15 times of water gagings at every turn, extracts 120min;
B, extracting solution is evaporated to 1500ml, concentrated solution discards ether layer with the clarification of extracted with diethyl ether to ether layer;
C, with water layer position reuse ethyl acetate extraction, reclaim ethyl acetate, 90 ℃ of vacuum drying temperature obtain 60% active parts of gallnut;
D, active parts of gallnut that step c is obtained are with gel chromatography column (model: Sephadex LH-20) carry out the separation of chemical constituent; Carry out gradient elution with water and 20%-100% methanol; The extractum I of 40%-70% meoh eluate and the extractum II of 70%-100% meoh eluate have been obtained; Again the extractum I is used model respectively with II: MCI-GEL CHP-20P gel chromatography column separates, with water and 20%-80% methanol gradient elution; The extractum I has obtained the chemical compound gallic acid ,-digallic acid and gallicin; The extractum II has obtained the extractum III and the extractum IV of 30%-60% meoh eluate; Respectively the extractum III is used model with the extractum IV again: Sephadex LH-20 gel chromatography column separates; Methanol with water and 20%-100% carries out gradient elution, in the meoh eluate of the 60%-100% of extractum III, has obtained chemical compound 1,2; 3,6-four-O-galloyl-beta-D-glucose; In 40%～60% meoh eluate of extractum IV, obtained chemical compound 1,2,3,4,6-five-O-galloyl-beta-D-glucose.
Active parts of gallnut adopts version pharmacopeia appendix X B content of tannin algoscopy in 2000, and the content of measuring tannin is greater than 50%.
Embodiment 4
A, get dry Galla Turcica (Galla Helepensis) pulverizing medicinal materials, cross sieve No. 2, take by weighing the 100g medicinal powder, extracting in water 3 times adds 12 times of water gagings at every turn, extracts 80min;
B, extracting solution is evaporated to 1100ml, concentrated solution discards ether layer with the clarification of extracted with diethyl ether to ether layer;
C, with water layer position reuse ethyl acetate extraction, reclaim ethyl acetate, 80 ℃ of vacuum drying temperature obtain 50% active parts of gallnut;
D, active parts of gallnut that step c is obtained are with conventional method gel chromatography (model: Sephadex LH-20) carry out the separation of chemical constituent; Carry out gradient elution with water and 20%-100% methanol; The extractum I of 40%-70% meoh eluate and the extractum II of 70%-100% meoh eluate have been obtained; Again the extractum I is used model respectively with II: MCI-GEL CHP-20P gel chromatography column separates, with water and 20%-80% methanol gradient elution; Extractum I has obtained the chemical compound gallic acid ,-digallic acid and gallicin; The extractum II has obtained the extractum III and the extractum IV of 30%-60% meoh eluate; Respectively the extractum III is used model with the extractum IV again: Sephadex LH-20 gel chromatography column separates; Methanol with water and 20%-100% carries out gradient elution, in the meoh eluate of the 60%-100% of extractum III, has obtained chemical compound 1,2; 3,6-four-O-galloyl-beta-D-glucose; In 40%～60% meoh eluate of extractum IV, obtained chemical compound 1,2,3,4,6-five-O-galloyl-beta-D-glucose.
Active parts of gallnut adopts version pharmacopeia appendix X B content of tannin algoscopy in 2000, and the content of measuring tannin is greater than 50%.
Active parts of gallnut adopts water to carry the back organic solvent extraction and can obtain active parts of gallnut as the purposes of the medicine of preparation treatment anti-ulcerative colitis effect, mainly comprises the tannin class; Effective site can obtain monomeric compound through the gel chromatography extraction separation, comprise gallic acid ,-digallic acid, gallicin, 1,2; 3,6-four-O-galloyl-beta-D-glucose, 1,2; 3; 4,6-five-O-galloyl-beta-D-glucose is processed oral formulations by conventional method with the active parts of gallnut that extracts.
Embodiment 5 active parts of gallnut are in the experimentation of preparation treatment anti-ulcerative colitis
Summary: purpose: the research Galla halepensis extract is to the therapeutical effect of rat ulcer colitis (UC) animal model.Method: adopt TNB and ethanol complex method to prepare rat UC model, after pathological section confirms the modeling success, be divided into Galla halepensis extract high and low dose group at random, model group and sulfasalazine group.Behind the gastric infusion 20d, estimate rat colon mucosa injury index (CMDI); Detect body weight, the heavy exponential sum immune organ weight of intestinal index variation; Detect superoxide dismutase (SOD), myeloperoxidase (MPO) (MPO) activity in the rat colon tissue.The result: Galla halepensis extract high dose group animal ulcer is healing fully all, compares with the model group animal, and SOD significantly increases (P＜0.01) in the high and low dose treated animal tissue, and MPO significantly reduces (P＜0.01).Conclusion: Galla halepensis extract has the obvious treatment effect to the rat ulcer colitis.
The present invention, observes its therapeutical effect and detects index of correlation after the Galla halepensis extract administration through setting up rat colitis model, understands the mechanism of action of its treatment ulcerative colitis.
Experiment material and method
Animal and grouping
50 of SPF level SD rats, male and female half and half, 200-250g, the unit of providing: Nanking Military Area Command of the Chinese People's Liberation Army medical animal experiment center, credit number: SCXK (Soviet Union) 2003-0004.Be divided into blank group, model group, positive controls and Galla halepensis extract high and low dose group at random, 10 every group.
Medicine and reagent
Active parts of gallnut extractum, Uigur Medical Inst., Uigur Autonomous Region, Xinjiang provides, lot number: 090926; 2,4,6-TNB (TNBS) is the Sigma Company products, 50% volume fraction, lot number: 046K5008; Dehydrated alcohol (AR) is Nanjing Chemistry Reagent Co., Ltd.'s product, lot number: 061110961; Ether (AR) is Nanjing Chemistry Reagent Co., Ltd.'s product, lot number: 060610483; The sulfasalazine enteric coatel tablets are Shanghai Sunve Pharmaceutical Co., Ltd.'s product, the accurate word of traditional Chinese medicines: H31020450, lot number: 200701C03; SOD test kit (surveying total superoxide dismutase), lot number: 20070516; Myeloperoxidase (MPO) (MPO) testing cassete, lot number: 20070516, above testing cassete builds up bio-engineering research institute product by Nanjing
The model preparation
60 of SD rats, male and female half and half, 200～250g, the labelling that divides into groups to weigh, fasting one day is freely drunk water.Modeling:, be made into modeling solution with the ethanol of 50% (V/V) and 5% TNBS equivalent mixing., and fixing with ether with rat anesthesia, one section diameter 2.0mm is about baby's catheter of 10cm, by the about deeply 8cm of the light and slow insertion of rat anus, slowly inject TNBS and alcohol mixed solution 1ml/, let the rat inclination a little of facing upward during injection, prevent leakage.In 2～3 days subsequently, observe the physiological change of rat.Diarrhoea appears, mucopurulent bloody stool modeling success.
Administration
From the 7th day beginning gastric infusion of preparation behind the model, every day 1 time, totally 20 days.Blank group and model group are irritated stomach and are given 10ml/kg normal saline.Positive controls: sulfasalazine enteric coatel tablets 100mg/kg, Galla halepensis extract high and low dose group dosage is respectively 1.68g/kg and 0.34g/kg.
The preparation of tissue specimen and index detect
Rat is put to death in rat administration the 21st day, gets colon, carries out morphologic observation and colon damage scoring (CMDI); The clip colon is weighed and is calculated the heavy index of intestinal.Picked at random part colon is fixed with the formalin solution of 10% (V/V), gets each three sections intestinal tube of lesions and front and back thereof then, through the flowing water flushing, and conventional preparation section, HE dyeing.Win spleen and thymus simultaneously, claim its weight, and calculate its organ index.Eyeball is got blood before putting to death rat, separate to obtain serum, and centrifugal 10 minutes of 3000r/min ,-4 ℃ of preservations are subsequent use, press the test kit description and measure SOD and MPO activity.
Statistical procedures
Adopt SPSS11.5 software processes statistical data data, adopt t check and F to analyze (q check) and analyze above-mentioned each each mean of group experiment.
Experimental result
Active parts of gallnut is to the influence of ulcerative colitis rat colon damage due to the TNBS
CMDI is the leading indicator of reaction colon degree of injury, and relatively there were significant differences (P＜0.01) for high dose group and positive drug group and model group, and low dose group and model group be no difference of science of statistics relatively.
Table 1 active parts of gallnut is to the influence of ulcerative colitis rat colon damage due to the TNBS
Compare with the model group group:
▲P＜0.05
▲ ▲P＜0.01.
Active parts of gallnut is to the heavy exponential influence of ulcerative colitis rat colon intestinal due to the TNBS:
Heavy index of active parts of gallnut high dose group and positive drug group intestinal and normal group be no significant difference (P＞0.05) relatively, significantly reduces with model group (P＜0.01); There were significant differences (P＜0.01) for heavy index of Galla halepensis extract low dose group intestinal and normal control group, with the apparent in view reduction of model group (P＜0.05).
Table 2 active parts of gallnut is to the heavy exponential influence of ulcerative colitis rat colon intestinal due to the TNBS
Compare with normal group: * * P＜0.05, * * P＜0.01; Compare with the model group group:
▲P＜0.05
▲ ▲P＜0.01
The mensuration of rat immunity organ relative weight
Compare with matched group, the immune organ of positive drug group, Galla halepensis extract high and low dose group does not all have significant difference (P＞0.05); Each group and the relatively more equal not statistically significant (P＞0.05) of model group.
Table 3 active parts of gallnut is to the influence of ulcerative colitis rat immunity organ due to the TNBS
Compare with normal group: * P＜0.05**P＜0.01; Compare with model group:
▲P＜0.05
▲ ▲P＜0.01.
Active parts of gallnut is to the active influence of UC rat blood serum MPO due to the TNBS:
Compare with model group, active significantly reduce (P＜0.01) of high dose group and positive drug group MPO, low dose group and model group be not statistically significant (P＞0.05) relatively; Compare positive drug, active significantly raise (P＜0.01) of Galla halepensis extract high and low dose group SOD with model group.
Table 4 active parts of gallnut is to MPO, the active influence of SOD in the ulcerative colitis rat blood serum due to the TNBS
Compare with normal group: * P＜0.05**P＜0.01; Compare with model group:
▲P＜0.05
▲ ▲P＜0.01.
Pathological examination results
The blank group: each Mus colon liner simple columnar epithelium, mucosa is complete, and goblet cell is abundant, is dispersed in the lymph-plasma cellular infiltration between mucosa, and part is distinguished visible lymph follicle under the mucosa, the equal Non Apparent Abnormality of flesh layer and placenta percreta.Model group: the wherein basic degeneration of 9 Mus mucous membrane of colon epitheliums, come off, replaced by large stretch of necrosis and inflammatory exudate, treating serious edema caused under the mucosa, under the mucosa, flesh layer, placenta percreta and serous coat are seen a large amount of inflammatory cell infiltrations outward, are main with neutrophilic granulocyte.In addition 1 Mus is inflammatory cell slightly increases in mucous membrane of colon, roughly with blank group Mus colon plesiomorphism.Positive drug administration group: each Mus colon form and blank group basically identical, the liner simple columnar epithelium, mucosa is complete; Goblet cell is abundant; Be dispersed in the lymph-plasma cellular infiltration between mucosa, part is distinguished visible lymph follicle under the mucosa, the equal Non Apparent Abnormality of flesh layer and placenta percreta.Low dosage administration group: wherein 7 routine Mus mucous membrane of colon are seen and are dispersed in a columnar epithelium degeneration, come off, mucosa down and the flesh layer see more acute and chronic cell infiltration, and have lymph follicle to form filling ulcer; All the other 3 routine Mus mucous membrane of colon forms and blank group basically identicals; Mucosa is complete, and goblet cell is abundant, is dispersed in the lymph-plasma cellular infiltration between mucosa; Part is distinguished visible lymph follicle under the mucosa, the equal Non Apparent Abnormality of flesh layer and placenta percreta.High dose administration group: 8 routine Mus mucous membrane of colon see the many places mucosa than the degeneration of large tracts of land epithelium, come off, inflammatory exudate and downright bad more under the mucosa, flesh layer and all visible a large amount of cell infiltration of placenta percreta, is main with neutrophilic granulocyte.All the other 2 routine Mus mucous membrane of colon forms and blank group basically identicals, mucosa is complete, and goblet cell is abundant, is dispersed in the lymph-plasma cellular infiltration between mucosa, and part is distinguished visible lymph follicle under the mucosa, the equal Non Apparent Abnormality of flesh layer and placenta percreta.
Discuss
At present; The common drug of treatment ulcerative colitis mainly comprises hormone (like cortical steroid), aminosallcylic acid class (as: SASP, 5-ASA) and immunosuppressant (like methotrexate), calcium ion antagonist, heparin etc.; But effect is not good enough, and untoward reaction is many, easy relapse after the drug withdrawal.Chinese traditional treatment is many from function of spleen and stomach regulating, nourishing liver and kidney Zhi Qiben, heat-clearing and toxic substances removing, and activating blood and removing stasis is Gu intestinal is controlled its mark.The all methods of administered through oral Chinese medicine, Chinese medicine and western medicine coupling and herbal retention enema on the method for treatment; All received certain curative effect in clinical; What is worth mentioning is that the oral results of comparison of extract oral and Western medicine is seen, the curative effect of Chinese medicine all is superior to using Western medicine merely, explains that Chinese medicine has its certain advantage in this disease of treatment; Simultaneously also received the curative effect same with oral medicine for herbal retention enema, and easy and simple to handle.Galla Turcica (Galla Helepensis) property is done cold, and bitter and puckery flavor has the effect of astringent or styptic treatment for spontaneous sweating, convergence, dampness, hemostasis, antiinflammatory, and research shows that Galla Turcica (Galla Helepensis) contains compositions such as tannin, flavone, gallic acid, and wherein the content of tannin of tannin extract surpasses 50%.Pharmaceutical research proves that Galla halepensis extract has stronger inhibition and killing action to staphylococcus aureus, escherichia coli, beta hemolytic streptococcus etc.; And can significantly suppress acute and chronic inflammation, and improve the body non-specific immunity, have clearing away heat and alleviating pain, remove free radical and suppress effect such as lipid peroxidation injury, it is clinical that to be usually used in treating ulcerative colitis evident in efficacy.
The present invention adopts TNBS and ethanol composite algorithm to make rat ulcer colitis model.TNBS is a kind of hapten material, and the mucus on dissolve with ethanol intestinal mucosa surface is destroyed intestinal mucosal barrier, becomes complete antigen after making TNBS and intestinal tissue macromolecular organization protein binding, causes being directed against the immunoreation of intestinal mucosa, brings out the generation of ulcerative colitis.Its pathological characteristic is that colon is a kind of chronic inflammatory disease variation, and the course of disease is longer, and pathological change is mutually similar with human ulcerative colitis.Test confirms that Galla halepensis extract (high and low dose group) has clear and definite therapeutical effect to rat colon ulcer due to the TNBS.Show Galla halepensis extract high dose group animal colon Non Apparent Abnormality from pathological examination; Model group rat colon mucosa and Submucosa inflammatory cell infiltration; With neutrophil infiltration is main, and part exuviation occurs and mucous layer is downright bad, tangible granuloma occurs.MPO is a kind of enzyme that is present in the neutrophilic granulocyte; Being the important indicator of neutrophil infiltration, also is the index of colitis degree of injury, and experimental result shows; Galla halepensis extract can obviously reduce MPO level in the rat model serum, explains that Galla halepensis extract has tangible antiinflammatory action.Radical damage is one of important mechanisms of UC morbidity; SOD is the important enzyme that body is removed oxygen-derived free radicals, and the oxidation and the antioxidation balance of body played crucial effects, and it can suppress the lipid peroxidation in the intestinal tissue; And ability stabilizing cell membrane; Experimental result shows that Galla halepensis extract has obviously improved SOD level in the rat peripheral blood, explains that it has the anti-oxidative damage ability.This research shows that active parts of gallnut has significant therapeutic effect to ulcerative colitis, and its mechanism maybe be relevant with antiinflammatory, the antioxidation of Galla halepensis extract.
Embodiment 6 active parts of gallnut are in preparation treatment antiinflammatory, analgesia and antibacterial experiment research
Summary: purpose: observe antiinflammatory, analgesia and the pharmacological action such as antibiotic of active parts of gallnut.Method: through the swelling of mice caused by dimethylbenzene xylene auricular concha, acetic acid induced mice abdominal cavity capillary permeability increase, carrageenin brings out mouse peritoneal leukoplania, carrageenin cause that mice pedal swelling, rat granuloma are swollen, the tests such as in-vitro antibacterial of acetic acid induced mice writhing response and common intestinal bacteria, observe antiinflammatory, analgesia and the antibacterial action of Galla halepensis extract.The result: active parts of gallnut mice (2.5,5.0,10.0ml/kg), rat (1.75,3.5,7.0ml/kg) gastric infusion obviously suppresses the swelling of xylene induced mice auricular concha; Mouse peritoneal capillary permeability due to the antagonism acetic acid increases; Rat abdominal cavity leukoplania due to the remarkable minimizing carrageenin; Obviously suppressing mice toes carrageenin causes swollen; To the swollen inhibitory action that is formed with of rat granuloma; Can obviously reduce acetic acid induced mice writhing response number of times.The in-vitro antibacterial test is respectively 0.0650%, 0.0650%, 0.0650%, 0.0650%, 0.0650%, 0.150%, 0.150%, 0.150%, 0.50% to the MBC (MBC) of 9 kinds of common index bacterium such as Salmonella typhimurium Pseudomonas, moscow' paratyphi B genus, Salmonella typhimurium genus, enterotoxigenic E.Coli, enteropathogenic E.Coli, enteroinvasive E.Coli, shigella dysenteriae genus, shigella flexneri genus, bacillus ceylonensis A genus.Conclusion: active parts of gallnut has tangible antiinflammatory, analgesic activity, and the common pathogen relevant to ulcerative colitis has bacteriostasis.
Galla Turcica (Galla Helepensis) is the larva of the insecticide gall wasps Cynips gallae-tinctoriceOliv of gall wasps section, parasitizes on the Fagaceae plant Galla Turcica (Galla Helepensis) tree Quercus infectoriaOliv. sprout insect gall that is produced.Galla Turcica (Galla Helepensis) property is done cold, and bitter and puckery flavor has the effect of astringent or styptic treatment for spontaneous sweating, convergence, dampness, hemostasis, antiinflammatory, is the Uygur medicine medicinal herbs most in use.The Uygur medicine theory thinks that it is directed against the intestinal wall absorbing power that colitis causes and the power that pinches descends, and directly acts on intestinal wall, holds back intestinal, and antiinflammatory antidiarrheal is opened the intestinal resistance, impels ulcer healing.The clinical treatment that is used for ulcerative colitis, evident in efficacy.But mechanism of action of its treatment ulcerative colitis is not clear and definite as yet, and this experiment is studied through antiinflammatory, analgesia and antibacterial action to active parts of gallnut, inquires into the mechanism of action that it treats ulcerative colitis, for the new drug development of Galla Turcica (Galla Helepensis) provides foundation.
Experiment material and instrument
Medicine and reagent
Receive the reagent thing: active parts of gallnut extractum (every milliliter contains crude drug 40mg), Uigur Medical Inst., Uigur Autonomous Region, Xinjiang provides, lot number: 090926.
Test reagent: xylene, analytical pure, Tianjin northization glass purchase and sale center (lot number 031228).Ivens is blue: Shanghai chemical reagent purchasing and supply station provides, Fluka import packing (lot number 82-11-02).Acetic acid: the many chemical reagent factories (lot number 030506) of Zhengzhou City's moral.Carrageenin: pharmacological room of Beijing Medical University provides.Index bacterium, Salmonella typhimurium Pseudomonas (50071), moscow' paratyphi B belong to (50602), Salmonella typhimurium belongs to (50115), enterotoxigenic E.Coli (44815), enteropathogenic E.Coli (44336), enteroinvasive E.Coli (44850), shigella dysenteriae genus (51570), shigella flexneri genus (51142), bacillus ceylonensis A genus (51592).Microbiology teaching and research room of Xinjiang Medicine University provides.
Nutrient agar, Hangzhou Milky Way biological reagent company limited product (lot number 200304).The MH broth bouillon, the health defence check of the military region, Zhejiang Province rear-service department provides (lot number 200304).The MH agar culture medium, Hangzhou Milky Way biological reagent company limited (lot number 200304).Normal saline: Shangdong Hualu Pharmaceutical Co., Ltd. (lot number H20030128).
Test apparatus
BS110S electronic balance: Beijing Sai Duolisi balance company limited.DT200 electronic balance: Shanghai Hengping Science Instrument Co., Ltd..JM31000 electronic balance: the Yuyao City inscription calibration equipment company limited of weighing of recording.Outside micrometer: Shanghai Measuring and Cutting Tools Plant.Electronic clinical thermometer: Fujian health Scientific and Technical Industry Co., Ltd.Stopwatch: Shanghai Venus instrucment and meter plant, QB/T1534-199.The biochemical incubator of FM: Shanghai Fuma Experiment Equipment Co., Ltd..The YJ-875 superclean bench: the cleaning equipment company limited is stayed in the sea, Wujiang.DM2500 micro imaging system: German Leica Company products.
Laboratory animal
Wistar and SD male rat, regular grade; Mice, Kunming kind, regular grade, male and female half and half.The certificate of competency is provided: moving word 16-003 number of Xinjiang doctor by Xinjiang Medicine University's Experimental Animal Center.
Method and result
Active parts of gallnut to mice auricular concha caused by dimethylbenzene xylene bullate influence get 50 of Kunming mouses, body weight 20 ± 2g, male and female half and half.Be divided into 5 groups at random, 10 every group, be blank group, positive controls, active parts of gallnut little, in, heavy dose of group; Once a day, continuously gastric infusion (ig) is 7 days, in the last administration after 1 hour; Xylene 50 μ l are smeared on every group of mouse right ear two sides, take off neck behind the 1h and put to death mice, get left and right sides auricle with the 8mm card punch; Weigh; With two auricle weight differences is the swelling degree, organizes a mean t check, and calculates the checking suppression ratio according to " (the average swelling degree of the blank group-average swelling degree of administration the group)/average swelling degree of blank group * 100% ".
The result: active parts of gallnut various dose gastric infusion, the swelling of xylol induced mice auricular concha has inhibitory action (result sees table 1) significantly.
Table 1 active parts of gallnut is to the bullate influence of mice auricular concha caused by dimethylbenzene xylene
Annotate: compare * * * P＜0.01, * * P＜0.05 with the blank group.
The influence that active parts of gallnut Dichlorodiphenyl Acetate induced mice abdominal cavity capillary permeability increases:
Get 50 of Kunming mouses, body weight 20 ± 2g, male and female half and half are divided into 5 groups at random, 10 every group, be blank group, positive controls, Galla halepensis extract little, in, heavy dose of group.Once a day, continuous gastric infusion is 7 days.1h after the last administration, each mouse tail vein injection 0.5% azovan blue normal saline solution 0.1ml/10g, and ip0.6% glacial acetic acid 0.2ml/ immediately; Put to death mice behind the 20min; With 5ml normal saline flushing abdominal cavity, collect cleaning mixture, cleaning mixture adds normal saline to 10ml; The centrifugal 15min of 3000rpm gets supernatant and measures its OD value in ultraviolet spectrophotometer 590nm wavelength.With the blue concentration of ivens in the OD value representation flushing liquor, reflect the degree of peritoneum inflammatory exudation indirectly.And the blank group is relatively organized a mean t check, and there was no significant difference is relatively arranged.And with " (the average OD value of the blank group-average OD value of administration the group)/average OD value of blank group * 100% " calculating suppression ratio.
As a result, each administration group abdominal cavity cleaning mixture OD value of active parts of gallnut is obviously low than matched group, shows that the mouse peritoneal capillary permeability that said preparation obviously suppresses due to the acetic acid increases (result sees table 2).
The influence that table 2 active parts of gallnut group increases the mouse peritoneal capillary permeability (X ± SD)
Annotate: compare * * * P＜0.01 with the blank group.
Active parts of gallnut causes the influence of swelling to mice foot sole of the foot carrageenin:
Get 50 of Kunming mouses, body weight 20 ± 2g, male and female half and half.Be divided into 5 groups at random, 10 every group, be blank group, positive controls, Galla halepensis extract little, in, heavy dose of group.Once a day, continuous gastric infusion is 7 days.1h after the last administration, central authorities are subcutaneous with the 2% carrageenin 0.06ml injection mice foot sole of the foot, cause scorching back 1h, 6h, 24h, survey two sufficient sole of the foot thickness with micrometer, so that sufficient sole of the foot thickness are the swelling degree before and after the inflammation, respectively organize mean t check.
As a result, active parts of gallnut various dose gastric infusion, on Carrageenan causes the mice pedal swelling has inhibitory action, and effect can be kept about 24 hours, wherein heavy dose of group apparent in view (result sees table 3).
Table 3 active parts of gallnut causes bullate influence (X ± SD) to mice foot sole of the foot carrageenin
Annotate: compare * P＞0.05, * * P＜0.05, * * * P＜0.01 with the blank group.
Active parts of gallnut is to the influence of rat abdominal cavity leukoplania:
Choose 60 of SD rats, body weight 200 ± 20g, male and female half and half.Be divided into 6 groups at random, 10 every group, be blank group, model group, positive controls, active parts of gallnut little, in, heavy dose of group.Blank group and model group rat oral gavage give normal saline, and positive controls is irritated the stomach phenacetin+caffeine+aspirin, tried thing little, in, heavy dose of group irritates the active parts of gallnut that stomach gives corresponding dosage respectively, once a day, continuous 7 days.1h after the last administration; Other group rats by intraperitoneal injection 2% carrageenin 0.4ml/ only puts to death animal after 5h hour, with 3ml normal saline flushing abdominal cavity except that matched group; Bar liquid 1ml is washed in collection; Placing in the small test tube, by the total white blood cells in each visual field of numeration of leukocyte method counting, is that index is organized a t check with the leukocyte count mean.
The result shows, active parts of gallnut various dose ig administration, and the rat abdominal cavity leukoplania has inhibitory action (result sees table 4) significantly due to the on Carrageenan.
Table 4 active parts of gallnut is to the influence
of rat abdominal cavity leukoplania
Annotate: compare with the blank group
△ △ △P＜0.01; Compare * * * P＜0.01 with model group.
Galla halepensis extract is to the bullate influence of rat granuloma:
Get 40 of Wistar rats, male and female half and half, body weight 200 ± 20g is divided into 5 groups at random, 8 every group.It is the active parts of gallnut administration group of normal group, 5-ASA group, 3 various dose.In the ether light anaesthesia, anaesthetize successfully after, the both sides footpath portion of every rat uses iodine disinfection; 75% cotton ball soaked in alcohol is respectively cut little a bite after taking off iodine, expands subcutaneous tissue with vascular forceps, implants subcutaneous from incision the autoclaving cotton balls of 20mg with the ophthalmology tweezers; With skin suture, postoperative began once a day the same day, ig7 days continuously; Put to death animal on the 8th day, and peeled off and take out the cotton balls granulation tissue, behind 60 ℃ of baking oven inner drying 12h, weigh; Deduct the raw cotton ball weight, be the granuloma net weight, and be converted to the 100g body weight with rat body weight and contain granuloma mg number; With t check between each group granuloma amount mean work group, and with " (blank group granuloma net (dry) weight-administration group granuloma the net (dry) weight)/clean weight in wet base of blank group granuloma * 100% " calculating suppression ratio.
The result shows, active parts of gallnut various dose gastric infusion is to the swollen inhibitory action (result sees table 5) significantly that is formed with of rat granuloma.
Table 5 active parts of gallnut is to the bullate influence of rat granuloma
Annotate: compare * * * P＜0.01 with the blank group.
Active parts of gallnut is to the influence of writhing response due to the mice acetic acid:
Get 50 of Kunming mouses, body weight 20 ± 2g, male and female half and half.Be divided into 5 groups at random, 10 every group, be blank group, positive controls, active parts of gallnut little, in, heavy dose of group.Once a day, continuous gastric infusion is 7 days.1h after the last administration, every Mus ip0.6% acetic acid 0.2ml/ only, that observes that every writhing response incubation period and 15 minutes behind the ip in mice acetic acid, endogenous cause of ill pain caused turns round the body number of times.And matched group is relatively organized a mean t check, and there was no significant difference is relatively arranged.And with " (the blank group is on average turned round body number of times-medication group and on average turned round the body number of times)/blank group is on average turned round body number of times * 100% " calculating analgesia percentage rate.
As a result, active parts of gallnut can obviously reduce acetic acid induced mice writhing response number of times, shows that said preparation has significant analgesic activity (result sees table 6).
The influence
of table 6 active parts of gallnut Dichlorodiphenyl Acetate induced mice writhing response
Annotate: compare * * * P＜0.01 with the blank group.
Active parts of gallnut is to the influence of nine kinds of common intestinal bacteria:
Index bacterium working concentration: 9 strain index bacterium recoveries is also gone down to posterity after 2 times, and under physiological saline solution, turbidimetry is made into working concentration and is about 1 * 10
6/ ml, dilution back inoculation simultaneously is dull and stereotyped, calculates viable count.Behind the medicine process filtering bacterium, make doubling dilution with MH meat soup, the packing small test tube, every pipe 1.8ml, the drug dilution degree was respectively 1: 1, and 1: 2,1: 4,1: 8,1: 16,1: 32,1: 64.Get above-mentioned antibacterial work bacterium liquid 0.2ml, add in the test tube of 1.8ml pastille meat soup, index bacterium ultimate density is about 1 * 10
6/ ml cultivated 24 hours for 37 ℃, and dull and stereotyped observation of inoculation has or not the respective fine bacteria growing respectively, calculates MBC (MBC), the test triplicate.Matched group: the aseptic contrast of culture medium, the aseptic contrast of medicine, the aseptic contrast of normal saline.
Index bacterium working concentration: 10
7Cfu/ml; Salmonella typhimurium Pseudomonas working concentration is 2.7 * 10
7Cfu/ml, index bacterium final concentration 2.7 * 10
6Cfu/ml; It is 6.2 * 10 that moscow' paratyphi B belongs to working concentration
7Cfu/ml, index bacteria concentration 6.2 * 10
6Cfu/ml; Salmonella typhimurium belongs to working concentration 2.0 * 10
7Cfu/ml, index bacteria concentration 2.0 * 10
6Cfu/ml; Enterotoxigenic E.Coli working concentration 0.6 * 10
7Cfu/ml, index bacteria concentration 0.6 * 10
6Cfu/ml; Enteropathogenic E.Coli working concentration 4.8 * 10
7Cfu/ml, index bacteria concentration 4.8 * 10
6Cfu/ml; Enteroinvasive E.Coli working concentration 0.7 * 10
7Cfu/ml, index bacteria concentration 0.7 * 10
6Cfu/ml; Shigella dysenteriae belongs to working concentration 7.0 * 10
7Cfu/ml, index bacteria concentration 7.0 * 10
6Cfu/ml; Shigella flexneri belongs to working concentration 2.1 * 10
7Cfu/ml, index bacteria concentration 2.1 * 10
6Cfu/ml; Bacillus ceylonensis A belongs to working concentration 1.8 * 10
7Cfu/ml, index bacteria concentration 1.8 * 10
6Cfu/ml.
The result shows that the MBC of 9 kinds of index bacterium (MBC) is respectively Salmonella typhimurium Pseudomonas 0.0650%, and moscow' paratyphi B belongs to 0.0650%; Shigella dysenteriae belongs to 0.0650%, and shigella flexneri belongs to 0.0650%, and bacillus ceylonensis A belongs to 0.0650%; Salmonella typhimurium belongs to 0.150%; Enterotoxigenic E.Coli belongs to 0.150%, and enteroinvasive E.Coli belongs to 0.150%, and enteropathogenic E.Coli belongs to 0.50%.Contrast: culture medium asepsis growth, normal saline asepsis growth, equal asepsis growth (table 7) behind 3 kinds of drug disinfections.
Table 7 active parts of gallnut is to the influence of nine kinds of common intestinal bacteria
Discuss
Galla Turcica (Galla Helepensis) is the Uygur medicine medicinal herbs most in use, records medical material standard-first volume in Uygur.Have astringent or styptic treatment for spontaneous sweating, dampness, hemostasis, antiinflammatory, anticorrosion effect.Be used for that asthenia of the large intestine is sliding, dysentery is more than, habitual enteritis, gingiva are lax, periodontitis, halitosis, pharyngolaryngitis etc.Xinjiang Uygur Autonomous Regions's Uygur medicine hospital clinical utilizes Galla halepensis extract, and stomachache, diarrhoea and the pyemia etc. of treatment due to the ulcerative colitis have obtained significant curative effect.
In vivo test research proof, Galla halepensis extract mice (2.5,5.0,10.0ml/kg), rat (1.75,3.5,7.0ml/kg) gastric infusion obviously suppresses the swelling of xylene induced mice auricular concha; Mouse peritoneal capillary permeability due to the antagonism acetic acid increases; Abdominal cavity leukoplania due to the minimizing carrageenin; The swelling of obvious suppression carrageenin induced mice toes; To the swollen inhibitory action significantly that is formed with of rat granuloma; Can obviously reduce acetic acid and cause the mouse writhing reaction times.The in-vitro antibacterial experimental study proves; Galla halepensis extract has inhibitory action to 9 kinds of common index bacterium such as Salmonella typhimurium Pseudomonas, moscow' paratyphi B genus, Salmonella typhimurium genus, enterotoxigenic E.Coli, enteropathogenic E.Coli, enteroinvasive E.Coli, shigella dysenteriae genus, shigella flexneri genus, bacillus ceylonensis A genus; Its MBC (MBC) is respectively Salmonella typhimurium Pseudomonas 0.0650%, and moscow' paratyphi B belongs to 0.0650%, and shigella dysenteriae belongs to 0.0650%; Shigella flexneri belongs to 0.0650%; Bacillus ceylonensis A belongs to 0.0650%, and Salmonella typhimurium belongs to 0.150%, and enterotoxigenic E.Coli belongs to 0.150%; Enteroinvasive E.Coli belongs to 0.150%, and enteropathogenic E.Coli belongs to 0.50%.
Above-mentioned research shows that Galla halepensis extract has tangible antiinflammatory, analgesic activity, and the common pathogen relevant to ulcerative colitis has bacteriostasis.
Embodiment 7
Active parts of gallnut is treated the clinical observation of anti-ulcerative colitis 48 examples in preparation:
Ulcerative colitis is a kind of chronic nonspecific ulcerative inflammation.Pathological changes mainly occurs in big intestinal mucosa and Submucosa, is main with ulcer and erosion, just is main clinical manifestation with diarrhoea, stomachache and the pus and blood mucus that shows effect repeatedly.At present should the disease sickness rate rise, treat thornyly, classified as one of modern difficult treatment by World Health Organization (WHO).The western medicine curative effect is dissatisfied, erious adverse reaction.Galla Turcica (Galla Helepensis) property is done cold, and bitter and puckery flavor has the effect of astringent or styptic treatment for spontaneous sweating, convergence, dampness, hemostasis, antiinflammatory, is the Uygur medicine medicinal herbs most in use, is mainly used in the treatment ulcerative colitis.Use active parts of gallnut treatment ulcerative colitis 48 examples, the result is reported as follows at present:
Document method
Physical data
48 examples are light, the moderate patients of ulcerative colitis of outpatient service, are divided into treatment group and matched group at random.24 examples are organized in treatment, male 16 examples, women 8 examples; Age 20-65 year, average (42.4 ± 12.5) year; Course of disease 1-22, average (7.3 ± 3.6) year; Slight 11 examples, moderate 23 examples.Matched group 24 examples, male 15 examples, women 9 examples; Age 20-62 year, average (41.7 ± 11.9) year: course of disease 1-20, average (6.8 ± 2.9) year: slight 12 examples, moderate 12 examples.Through statistical procedures, 2 groups are not being had significant difference (P＞0.05), tool comparability aspect sex, age, the course of disease and the severity extent.
Include standard in
All cases all have in various degree diarrhoea, stomachache and pus and blood mucus just, meet diagnostic criteria and therapeutic evaluation standard that the whole nation, Chengdu in 2000 inflammatory bowel scientific seminar formulates.All cases are light, the moderate patient who meets this standard, all diarrhoea＞6 time/d, have severe mucus hemafecia, body temperature more than 37.5 ℃, the severe patient of pulse＞90 time/min, hemoglobin＜100g/L, erythrocyte sedimentation rate＞30mm/h all forecloses.
Therapeutic Method
Treatment group: use active parts of gallnut 1.0g, every day 4 times.
Matched group: oral sulfasalazine 1.0g, every day 4 times; Change 1.0g after the remission into, every day 2 times.2 groups of cases all treated for 8 weeks.
Observation index and method
Observe symptom, signs such as treatment front and back patient's diarrhoea, stomachache and bloody purulent stool and improve situation; Mucosa variation, biological tissue pathologic finding under stool routine examination, routine blood test, the colonoscope; Observe untoward reaction.
Criterion of therapeutical effect
Adopt national chronic noninfective intestinal tract disease scientific seminar standard in 1993: promptly 1. cure in the recent period: clinical symptom disappearance, colonoscope check mucosa is normal.Drug withdrawal or only use the maintenance dose medicine is observed and was not had recurrence in 6 months.2. effective: clinical symptoms disappears basically, and mild inflammation reaction of colonoscope check mucosa and the false polyp of part form.3. invalid: clinical symptoms, scope and pathologic finding do not have improvement after treatment.
Statistical method
Carry out χ with the SPSS12.0 statistical software
2Check.
Observed result
Curative effect is relatively seen table 1.
Table 1 liang group clinical efficacy is (n=24) relatively
| Group | Cure in the recent period | Effectively | Invalid | Total effective rate (%) |
| The treatment group | 16(66.7％) | 6(25.0％) | 2(8.3％) | 91.7 ＊ |
| Matched group | 12(50.0％) | 7(29.2％) | 5(20.8％) | 79.2 |
*: p＜0.01, compare with matched group.
Discuss
Ulcerative colitis is a kind of comprehensive syndrome of colonic pathological change of changing into principal character with chronic nonspecific inflammation.Primary disease is prone to show effect repeatedly, delay and difficultly heals, complication is more.The cause of disease that causes ulcerative colitis has multiple theory, like theories such as immune factor, inherited genetic factors, Nervous and Mental Factors, infective agent, protection material want factors.Research recently shows that superoxide dismutase (SOD) activity is lower in ulcerative colitis patient's intestinal wall mucosa.Galla Turcica (Galla Helepensis) property is done cold, and bitter and puckery flavor has the effect of astringent or styptic treatment for spontaneous sweating, convergence, dampness, hemostasis, antiinflammatory, is the Uygur medicine medicinal herbs most in use.The Uygur medicine theory thinks that it is directed against the intestinal wall absorbing power that colitis causes and the power that pinches descends, and directly acts on intestinal wall, holds back intestinal, and antiinflammatory antidiarrheal is opened the intestinal resistance, impels ulcer healing.The clinical treatment that is used for ulcerative colitis, evident in efficacy.
Clinical treatment observation is the result show, active parts of gallnut has the obvious treatment effect to ulcerative colitis, total effective rate 91.7%, and cure rate 66.7% effectively is 25% in the recent period, invalid is 8.3%, obviously is superior to matched group.Pharmacological research shows that active parts of gallnut has the obvious treatment effect to the rat ulcer colitis; Can significantly increase SOD content in the chronic ulcer tissue (P＜0.01), reduce MPO content (P＜0.01), and tangible antiinflammatory, analgesic activity are arranged, the common pathogen relevant to ulcerative colitis has bacteriostasis.Active parts of gallnut is the good medicine of treatment ulcerative colitis.
Claims (1)
1. an active parts of gallnut is characterized in that in the purposes of the medicine of preparation anti-ulcerative colitis the method for distilling of active parts of gallnut follows these steps to carry out:
A, get dry Galla Turcica (Galla Helepensis) pulverizing medicinal materials, cross sieve No. 2, take by weighing the 100g medicinal powder, extracting in water 3 times adds 5-15 times of water gaging at every turn, extracts 20-120min;
B, extracting solution is evaporated to 500-1500ml, concentrated solution discards ether layer with the clarification of extracted with diethyl ether to ether layer;
C, with water layer position reuse ethyl acetate extraction, reclaim ethyl acetate, 45-90 ℃ of vacuum drying temperature obtains the active parts of gallnut of 30-60%; This effective site is carried out the separation of chemical constituent with Sephadex LH-20 type gel chromatography column; Carry out gradient elution with water and 20%-100% methanol; The extractum I of 40%-70% meoh eluate and the extractum II of 70%-100% meoh eluate have been obtained; Again extractum I is separated with MCI-GEL CHP-20P gel chromatography column respectively with II, with water and 20%-80% methanol gradient elution; Extractum I has obtained the chemical compound gallic acid ,-digallic acid and gallicin; Extractum II has obtained the extractum III and the extractum IV of 30%-60% meoh eluate; Respectively extractum III is separated with Sephadex LH-20 gel chromatography column with extractum IV again; Methanol with water and 20%-100% carries out gradient elution, in the meoh eluate of the 60%-100% of extractum III, has obtained chemical compound 1,2; 3,6-four-O-galloyl-beta-D-glucose; In the meoh eluate of the 40%-60% of extractum IV, obtained chemical compound 1,2,3,4,6-five-O-galloyl-beta-D-glucose.
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