CN103830288B - Match certain herbaceous plants with big flowers extractive of general flavone and its production and use - Google Patents
Match certain herbaceous plants with big flowers extractive of general flavone and its production and use Download PDFInfo
- Publication number
- CN103830288B CN103830288B CN201410089748.3A CN201410089748A CN103830288B CN 103830288 B CN103830288 B CN 103830288B CN 201410089748 A CN201410089748 A CN 201410089748A CN 103830288 B CN103830288 B CN 103830288B
- Authority
- CN
- China
- Prior art keywords
- herbaceous plants
- certain herbaceous
- big flowers
- general flavone
- match certain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention provides match certain herbaceous plants with big flowers extractive of general flavone, the weight percentage of general flavone is in terms of rutin, no less than 1%.Present invention also offers the preparation method of the extract and purposes.Present invention match certain herbaceous plants with big flowers extractive of general flavone steady quality, controllable, extraction process is simple and feasible, free from environmental pollution, is adapted to extensive extract.Match certain herbaceous plants with big flowers extractive of general flavone is used to treat prostatic disorders, especially treats hyperplasia of prostate, prostatitis or prostate cancer and non-bacterial prostatitis, clearly, safety provides a kind of new medication selection to drug effect to be clinical.
Description
Technical field
The present invention relates to match certain herbaceous plants with big flowers extract, belong to drug field.
Background technology
Match certain herbaceous plants with big flowers Malvastrum coromandelianum (L.) Garcke is Malvaceae semishrub plant, alias sweet vernal grass
Or Coromadel Coast Falsemallow Herb.For perennial Malvales draft, originate in America, now be widely distributed in the whole world tropical area.China is mainly distributed
In Taiwan, Fujian, Guangdong, Hong Kong, Hainan, Guangxi, Yunnan.Certain herbaceous plants with big flowers is matched with all herbal medicine, property is bitter, light, cool.With clearing heat and promoting diuresis,
The effect of removing toxic substances dissipates the stasis of blood.Cure mainly damp-heat dysentery, it is jaundice, cough with lung heat, abscess of throat, hemorrhoid, carbuncle sore tumefacting virus, traumatic injury, preceding
Row adenositis.Among the people to be commonly used to treat icteric hepatitis, pharmacological evaluation has been reported that proof match certain herbaceous plants with big flowers has antipyretic-antalgic antiinflammatory action（Field
Brightness, etc. the pharmacognostical study of match certain herbaceous plants with big flowers, Chinese medicine the 8th phase in August, 2006 of volume 29）.
Pertinent literature there is no to report currently for the active component or active component in match certain herbaceous plants with big flowers.
The content of the invention
The technical scheme is that there is provided a kind of match certain herbaceous plants with big flowers extract.Another technical scheme of the present invention there is provided this
The preparation method and purposes of extract.
Certain herbaceous plants with big flowers extractive of general flavone is matched the invention provides one kind, the weight percentage of general flavone is no less than in terms of rutin
1%.Further, the weight percentage of general flavone is in terms of rutin, no less than 3%；It is further preferred that the weight hundred of general flavone
Point content is in terms of rutin, no less than 20%；It is further preferred that the weight percentage of general flavone is no less than in terms of rutin
55%, it is no less than 50% with myricetin content meter.
It is to be extracted to be prepared from by water or organic solvent.
It is further preferred that described organic solvent is the ethanol that concentration is more than 70%.
Present invention match certain herbaceous plants with big flowers extractive of general flavone is by by water or organic solvent, being carried using refluxing extraction, surname extraction, ultrasound
Take, Microwave Extraction, supercritical CO2Extraction, dipping or percolation extraction method are prepared from.
Present invention also offers a kind of method for preparing described match certain herbaceous plants with big flowers extractive of general flavone, it comprises the following steps：
A, weigh match certain herbaceous plants with big flowers, add water or organic solvent immersion；
B, using heating water bath refluxing extraction, surname extraction, ultrasonic extraction, Microwave Extraction, supercritical CO2Extraction, dipping
Or percolation extraction method；Residue is washed with coordinative solvent, contract filtrate, filtering, produces present invention match certain herbaceous plants with big flowers extractive of general flavone.
Wherein, described organic solvent is more than 70% ethanol.
Wherein, in addition to step c：Purifying, specific purification process is as follows：
1. after the AB-8 type macroreticular resins that crude drug amount is soaked with ethanol, upper prop is pre-processed, with the second of volume fraction 95%
Alcohol flowing cleaning, the ethanol extremely flowed out to ethanol and water 1：Untill the not white muddiness of 5 mixing, then with distilled water flushing
To water lotion without alcohol taste；
2. the match certain herbaceous plants with big flowers extract solution loading of b step is taken, first with the water elution of 4 times of column volumes, eluent is discarded；After with 4 times of posts
The ethanol elution of volume 10%, discards eluent；Then with 8 times 40% of ethanol elution, eluent is collected, ethanol is recovered under reduced pressure,
It is concentrated to dryness, produces match certain herbaceous plants with big flowers general flavone；The weight percentage of general flavone is in terms of rutin, no less than 55%, with myricetin content meter
No less than 50%.
Present invention also offers use of the described match certain herbaceous plants with big flowers extractive of general flavone in the medicine for preparing treatment prostatic disorders
On the way.
Wherein, described medicine is the medicine for treating hyperplasia of prostate, prostatitis or prostate cancer.
It is the medicine for treating non-bacterial prostatitis present invention also offers described medicine.
Present invention match certain herbaceous plants with big flowers extractive of general flavone steady quality, controllable, extraction process is simple and feasible, free from environmental pollution, is adapted to
It is extensive to extract.Match certain herbaceous plants with big flowers extractive of general flavone is used to treat prostatic disorders, especially treat hyperplasia of prostate, prostatitis or
Prostate cancer and non-bacterial prostatitis, clearly, safety provides a kind of new medication selection to drug effect to be clinical.
Brief description of the drawings
The pathological change of Fig. 1 each group rat prostate tissues
Fig. 2 each group rat prostate tissue pathological changes
Embodiment
Embodiment 1 matches the preparation of certain herbaceous plants with big flowers extract（Reflux extraction）
Match certain herbaceous plants with big flowers 20g is weighed, is separately added into after 200ml 70% ethanol immersion 30min, heating and refluxing extraction 2 in water-bath
The secondary each 1h of, are filtered while hot, residual washing-out 2~3 times, and merging filtrate is recycled to no alcohol taste, are dissolved in water to every 1mL and are contained 0.5g
Crude drug, centrifuges (4000r/min) 15min, and filtering is standby.Take 1.0mL to take to be placed in 25mL measuring bottles, add water 6.0mL；Add 5%
(mass fraction) sodium nitrite solution 1.0mL, makes mixing, places 6min；10% (mass fraction) aluminum nitrate 1.0mL is added, is shaken
It is even, place 6min；4% (mass fraction) NaOH solution l0.0mL is added, scale is added water to, shakes up, 15min is placed；According to point
Light photometry (annex VB of Chinese Pharmacopoeia 2010 edition) determines absorbance A at 500nm wavelength.General flavone in sample solution
Recovery rate (general flavone recovery rate=general flavone quality/match certain herbaceous plants with big flowers quality × 100%).It is about 3.0g to match certain herbaceous plants with big flowers total extract quality, always
The content of flavones is calculated as 0.526g with rutin, accounts for the 17.5% of total extract, and general flavone recovery rate is 2.63%.
Embodiment 2 matches the preparation of certain herbaceous plants with big flowers extract（Soxhlet extraction）
Match certain herbaceous plants with big flowers 5g is weighed, is separately added into after 50ml 70% ethanol immersion 30min, by temperature control at 85 DEG C, extracts 4h,
Suction filtration while hot.Residue is washed 2~3 times with coordinative solvent, and merging filtrate is recycled to no alcohol taste, is dissolved in water to every 1mL and is contained 0.5g
Crude drug, centrifuges (4000r/min) 15min, and filtering is standby.By above-mentioned spectrophotometry general flavone, this method extracts what is obtained
It is about 0.9g to match certain herbaceous plants with big flowers total extract quality, and the content of general flavone is calculated as 0.140g with rutin, accounts for total extract 16%, general flavone is carried
It is 2.79% to take rate.
Embodiment 3 matches the preparation of certain herbaceous plants with big flowers extract（Ultrasonic extraction）
Match certain herbaceous plants with big flowers 20g is weighed, is separately added into after 200ml 70% ethanol immersion 30min, ultrasound（300W）Extraction 2 times, every time
30min, while hot suction filtration.Residue is washed 2~3 times with coordinative solvent, and merging filtrate is recycled to no alcohol taste, is dissolved in water to every 1mL
Crude drug containing 0.5g, centrifuges (4000r/min) 15min, and filtering is standby.By above-mentioned spectrophotometry general flavone, this method is extracted
Obtained match certain herbaceous plants with big flowers total extract quality about 3.5g, the content of general flavone is calculated as 0.716g with rutin, accounts for total extract 20%, always
Extracting flavonoids rate is 3.58%.
Embodiment 4 matches the preparation of certain herbaceous plants with big flowers extract（Microwave loss mechanisms）
Match certain herbaceous plants with big flowers 5g is weighed, is separately added into after 200ml 70% ethanol immersion 30min, microwave（400W）5min is extracted, while hot
Suction filtration, residue is washed 2~3 times with coordinative solvent, and merging filtrate is recycled to no alcohol taste, is dissolved in water to every 1mL crude drugs containing 0.5g,
(4000r/min) 15min is centrifuged, filtering is standby.By above-mentioned spectrophotometry general flavone, this method extracts obtained match certain herbaceous plants with big flowers
Total extract quality is about 2.0g, and the content of general flavone is calculated as 0.149g with rutin, accounts for total extract 7%, and general flavone recovery rate is
2.97%.
Embodiment 5 matches the preparation of certain herbaceous plants with big flowers extract（Supercritical CO2Extraction）
Match certain herbaceous plants with big flowers 5g is weighed, is fitted into extraction kettle, using 1mL/g absolute ethyl alcohol as entrainer, 60 DEG C of extraction temperature is extracted
Pressure 35MPa, extraction time 1h, CO2Flow 30kg/h-1CO2Extracted in supercritical extract system, collect extract, reclaimed
To without alcohol taste, being dissolved in water to every 1mL crude drugs containing 0.5g, (4000r/min) 15min is centrifuged, filtering is standby.By above-mentioned light splitting
Photometry determines general flavone, and this method extracts obtained match certain herbaceous plants with big flowers total extract quality about 2.0g, and the content of general flavone is in terms of rutin
For 0.099g, total extract 5% is accounted for, general flavone recovery rate is 1.97%.
Embodiment 6 matches the preparation of certain herbaceous plants with big flowers extract（Infusion process）
Match certain herbaceous plants with big flowers 5g is weighed, in Soakage extraction under 70% ethanol, solid-liquid ratio 1: 20, dipping 6h, room temperature condition, suction filtration, residue is used
Coordinative solvent is washed 2~3 times, and merging filtrate is recycled to no alcohol taste, is dissolved in water to every 1mL crude drugs containing 0.5g, is centrifuged (4000r/
Min) 15min, filtering is standby.By above-mentioned spectrophotometry general flavone, this method extracts obtained match certain herbaceous plants with big flowers total extract quality
About 2.0g, the content of general flavone is calculated as 0.066g with rutin, accounts for total extract 3%, and general flavone recovery rate is 1.31%.
Embodiment 7 matches the preparation of certain herbaceous plants with big flowers extract（Permeating extraction）
Match certain herbaceous plants with big flowers 20g is weighed, with being fitted into after 80% ethanol wet in diacolation post, starts diacolation after solubilizer immersion 24h, controls
Flow velocity 3mL/min, collects percolate, is recycled to no alcohol taste, is dissolved in water to every 1mL crude drugs containing 0.5g, centrifuges (4000r/min)
15min, filtering is standby.By above-mentioned spectrophotometry general flavone, this method extracts obtained match certain herbaceous plants with big flowers total extract quality and is about
2.5g, the content of general flavone is calculated as 0.414g with rutin, accounts for total extract 17%, and general flavone recovery rate is 2.07%.
Embodiment 8 matches the purifying of certain herbaceous plants with big flowers general flavone
After the AB-8 type macroreticular resins 24h that 4 times of crude drug amounts are soaked with ethanol, upper prop is pre-processed, and uses volume fraction
The flowing cleaning of 95% ethanol, the ethanol extremely flowed out to ethanol and water 1：Untill the not white muddiness of 5 mixing, then with distillation
Water is rinsed to water lotion without alcohol taste.Taking match certain herbaceous plants with big flowers extract solution 40mL, (per 1mL crude drugs containing 0.5g, general flavone content must not be less than on 1%)
Sample, first with the water elution of 4 times of column volumes, discards eluent；After the ethanol elution with 4 times of column volumes 10%, eluent is discarded；So
Afterwards with 8 times 40% of ethanol elution, eluent is collected, ethanol is recovered under reduced pressure, is concentrated to dryness, general flavone is produced.It is total that purifying is obtained
The recovery rate of flavones is about 3%, is 84% from the rate of transform in extract solution.
Wherein, what extracting method obtained in embodiment 1-7 is all crude flavonoid powder, by the purifying of embodiment 8, obtains the present invention
The percentage composition of general flavone in certain herbaceous plants with big flowers general flavone, described match certain herbaceous plants with big flowers general flavone is matched, in terms of rutin, no less than 55%, in terms of myricetin not
50% must be less than.
Concrete content assay method and detection data are：
Using rutin as reference substance, certain herbaceous plants with big flowers general flavone content is matched using determined by ultraviolet spectrophotometry.I.e. precision weighs rutin pair
According to product 23.0mg, put in 50mL measuring bottles, plus 95% EtOH Sonicate dissolves and is diluted to scale, shakes up, it is standby.Precision draws control
Product solution 1.0,2.0,3.0,4.0,5.0,6.0mL, are respectively placed in 25mL measuring bottles, add water to 6.0mL；Add 5% natrium nitrosum
Solution 1.0mL, makes mixing, places 6min；10% aluminum nitrate 1.0mL is added, is shaken up, 6min is placed；Add 4% sodium hydroxide
Solution 10.0mL, adds water to scale, shakes up, and places 15min；According to AAS (annex VB of Chinese Pharmacopoeia 2010 edition) in
Absorbance A is determined at 500nm wavelength, regression equation is calculated.Accurate pipette samples solution 1.0mL is put in 10mL measuring bottles,
Accurate draw solution 1.0mL again, by the above process, from " determining absorbance, substitution regression equation meter adding water to 6.0mL " in accordance with the law
Calculate the percentage composition of general flavone in sample solution.（Content/total extract of percentage composition=general flavone of general flavone）
Through three experiments, it is respectively 58%, 54%, 61% that purifying, which obtains match certain herbaceous plants with big flowers general flavone percentage composition, and it is always yellow as a result must to match certain herbaceous plants with big flowers
Ketone mean percent content is about 58%.
Using myricetin as reference substance, certain herbaceous plants with big flowers general flavone content is matched using determined by ultraviolet spectrophotometry.Take myricetin reference substance
14.18m g, are placed in 25mL volumetric flasks, add methanol dissolving, and the standard that concentration is 0.1592mg/mL is made in 25mL in constant volume
Product storing solution, precision measures the μ L of myricetin standard items storing solution appropriate 0,4,8,12,16,20, is respectively placed in 1mL volumetric flasks.
5% aluminium chloride 3mL is separately added into, with methanol constant volume in 10mL, 20min is placed, retinue reagent is blank, is surveyed at 270nm wavelength
Determine absorbance, calculate regression equation.Accurate pipette samples solution 1.0mL is put in 10mL measuring bottles, then accurate draw solution
1.0mL, by the above process, determines absorbance, substitutes into the percentage composition that regression equation calculation goes out general flavone in sample solution.（Always
Content/total extract of percentage composition=general flavone of flavones）
Through three experiments, obtained match certain herbaceous plants with big flowers general flavone percentage composition difference 49%, 53%, 51% is purified, as a result certain herbaceous plants with big flowers must be matched always yellow
Ketone mean percent content is 51%.
Beneficial effects of the present invention are proved below by way of specific pharmacodynamics test or experiment in vitro.
Test example 1 matches protective effect of the certain herbaceous plants with big flowers extract to non-bacterial prostatitis
1 material
1.1 animal SD rats, cleaning grade, male, 180~220g；KM mouse, cleaning grade, male, 18~22g of body weight,
By Fujian University of Traditional Chinese Medicine, Experimental Animal Center is provided.
1.2 medicines match certain herbaceous plants with big flowers extract with reagent（Embodiment 3 is made）；Prostat is given birth to by Meirui Pharmaceutical Co., Ltd, Nanjing
Production, lot number 20070514；XIAOZHILING ZHUSHEYE is produced by Shuanghe High-Tech. Natural Medicine Co., Ltd., Beijing, lot number
061212；Carrageenan is purchased from Sigma companies；Furosemide injection (SN) is purchased from Changzhou Kangpu Pharmaceutical Co., Ltd., ELISA reagents
Box is provided by Uscn Life Science Inc. companies.
2 methods
Protective effect of the 2.1 match certain herbaceous plants with big flowers extracts to chronic non-bacterial prostatitis will wipe out lower abdomen after SD rat anesthesias
Hair, aseptically abdominal incision go deep into abdominal cavity, exposure prostate is injected to 2 prostates with 25% XIAOZHILING ZHUSHEYE
Lateral lobe, suturing them.Rat was randomly divided into 6 groups, i.e. blank group in postoperative 7th day, model group matches basic, normal, high dose of certain herbaceous plants with big flowers extract
Every group 10,30d is administered by body weight continuous gavage in amount group (8,4,2gkg-1), positive drug Prostat group (60mgkg-1),
It is administered once daily, blank group and model group give normal saline.Put to death rat after last dose, abdominal aortic blood, from
The heart, takes serum, and blood is taken using the content each group rat aorta of ELISA method detection rat blood serum IL-1 β, IL-2 and TNF-α,
2000 × g centrifuges 10min, takes serum.Operation is carried out in strict accordance with kit specification.Prostata tissue is taken, is visually observed
Prostatic lesion state simultaneously claims weight in wet base, calculates prostate index；The μ L of massage of prostate liquid 10 are taken to be put into leucocyte dilution, light
Learn micro- Microscopic observation, counted for total White；A drop prostatic fluid smear separately is taken, Microscopic observation lecithin density is commented
Minute mark standard is:The full visual field (+++) of lecithin is 4 points, and 1/2 visual field (++) is 3 points, and 1/4 visual field (+) is 2 points, under low power lens
It is rarely seen it is several be 1 point.Opposite side lateral lobe of prostate gland is taken, fixed, transparent, the paraffin of conventional dehydration is placed in 10% formalin solution
A part of HE that cuts into slices is dyed, in tissues observed metamorphosis under light microscope.
2.2 carrageenans cause the experiment of rat acute prostatitis that SD rats (350~400g) are randomly divided into 6 groups, i.e., empty
White group, model group, match certain herbaceous plants with big flowers extract low dose group (2g/kg), match certain herbaceous plants with big flowers extract middle dose group (4g/kg), match certain herbaceous plants with big flowers extract are high
Dosage group (8g/kg), positive drug Prostat group (60mg/kg), every group 10.7d is administered by the advance continuous gavage of body weight, daily
It is administered once, blank group and model group give normal saline.30min after administration in 6th day, by rat anesthesia, lower abdomen is just
Middle otch, exposure prostate, the seminal fluid capsule on lateral lobe of prostate gland injects 0.1% sterile carrageenan normal saline solution, then
Suturing them.Weighed after 24h, put to death rat, taken prostate and claim weight in wet base, calculate prostate index；Take the μ of massage of prostate liquid 10
L is put into leucocyte dilution, optical microphotograph Microscopic observation, counted for total White (WBC)；It is another to take a drop prostatic fluid to apply
Piece, Microscopic observation lecithin density, standards of grading are：The full visual field (+++) of lecithin is 4 points, and 1/2 visual field (++) is
3 points, 1/4 visual field (+) be 2 points, under low power lens it is rarely seen it is several be 1 point.
KM mouse are randomly divided into 6 groups, i.e. blank group, model group, match certain herbaceous plants with big flowers extraction by the experiment of 2.3 mice caused by dimethylbenzene xylene ear swellings
Thing low dose group (2g/kg), match certain herbaceous plants with big flowers extract middle dose group (4g/kg), match certain herbaceous plants with big flowers extract high dose group (8g/kg), positive drug
Prostat group (60mg/kg), every group 10.Per mouse, left ear two sides, which is applied, causes scorching liquid 0.05mL, and auris dextra makees blank control.Difference gavage
7d is administered, is administered once a day, blank group and model group give normal saline.Put to death small after last day administration 0.5h
Mouse, is punched at same position respectively, and assay balance is weighed, and calculates swelling rate.
2.4 mouse granuloma induced by implantation of cotton pellets experiment by KM mouse be randomly divided into 6 groups, i.e. blank group, model group, match certain herbaceous plants with big flowers extract it is low
Dosage group (2g/kg), match certain herbaceous plants with big flowers extract middle dose group (4g/kg), match certain herbaceous plants with big flowers extract high dose group (8g/kg), positive drug house Buddhist nun
Logical group (60mg/kg), every group 10.In addition to blank group, mouse armpit subcutaneous operation implantation sterilizing cotton balls (10.0 ± 0.5) mg.
7d is administered in continuous gavage, is administered once a day, blank group and model group give normal saline.Weighed after 7d, put to death mouse.
Granulation tissue is peeled off, dries, weighs in 60 DEG C of baking ovens, granuloma weight is calculated.
The influence of 2.5 pairs of female mice hot plate induced pains takes 20 ± 2g of body weight female mice, by hot plate method in experiment before
Mouse 60 of the pain threshold in 5s-30s is determined on hot plate pain threshold detector, by body weight be randomly divided into 6 groups, i.e. blank group, model group,
Match certain herbaceous plants with big flowers extract low dose group (2g/kg), match certain herbaceous plants with big flowers extract middle dose group (4g/kg), match certain herbaceous plants with big flowers extract high dose group (8g/
Kg), positive drug Prostat group (60mg/kg), every group 10.To there is licking metapedes reaction for observation index.Filled respectively at every mouse
Stomach above medicine, 0.2mL/ is only.Pain threshold is determined by upper method after 0.5h, is then administered by preceding method, 1h, 1.5h, 2h pain is determined
Threshold value.Compare the change of the threshold of pain before and after administration, and by average pain threshold before (after medication-medication before average pain threshold)/medication ×
100%, which calculates the threshold of pain, improves percentage.
Healthy SD male rat (180~220g) is pre-placed 1 in metabolic cage by the experiment of 2.6 Water l oad rat urine amounts
~2d adapts to environment, is selected from the stable rat 50 of urine volume under conditions of water drinking, is randomly divided into Normal group, positive control
Group (furosemide injection 10mg/kg) and the match basic, normal, high dosage group of certain herbaceous plants with big flowers extract, every group 10.7d is administered in continuous gavage, finally
Water is can't help in 18h fasting before administration in one day, and each group presses 2mL/100g body weight dose intraperitoneal injection of saline before administration, then gavage
Administration, control group gives equivalent distilled water.Rat is put into metabolic cage immediately after administration, per one, cage, 1, after 2,4h respectively
Measure the urine volume of each group animal.
2.7 statistical procedures carry out statistical procedures using SPSS statistical softwares.Data withRepresent, compare between two groups
Relatively use independent samples t test, multigroup is compared and use one-way analysis of variance.
3 results
The influence finding of naked eye prostate group for the chronic non-bacterial prostatitis that 3.1 match certain herbaceous plants with big flowers extracts are induced xiaozhiling injection
Knit outward appearance:Blank group rat prostate tissue pinkiness, matter is soft, glossy, with surrounding tissue without adhesion；Before model group rats
Row gland body of gland is in kermesinus or brown color, generally with surrounding tissue adhesion, and matter is hard, has obvious scleroma, and have oedema；Prostat
Group rat prostate body of gland and surrounding tissue adhesion are lighter, and part body of gland matter is hard, and nodosity, color is pale brown；Match certain herbaceous plants with big flowers extract 8,
4gkg-1 dosage groups prostate gland is light with surrounding tissue adhesion, hardens small, and part body of gland is in brown color, there is Mild edema,
Indivedual bodies of gland are soft, ruddy, glossy, and outward appearance is similar to normal prostate tissue.
Match the antiinflammatory action of certain herbaceous plants with big flowers extract:Model group is compared with blank group, and the increase of WBC numbers, lecithin are small in prostatic fluid
Volume density is reduced, and the pathological change of chronic prostatitis is presented, and shows modeling success.Match certain herbaceous plants with big flowers extract 8gkg-1 dosage groups with
Model group compares, and WBC numbers are reduced, the increase of lecithin density, show that the chronic prostatitis that the medicine is induced xiaozhiling injection has
Certain antiinflammatory action, is shown in Table 1.
The influence for the rat chronic non-bacterial prostatitis that the match certain herbaceous plants with big flowers extract of table 1 is induced xiaozhiling injection
Note:Compared 1) P with blank group<0.01；Compared 2) P with model group<0.05,3) P<0.01.
The pathological change of prostata tissue:Blank group rat prostate major part lumen of gland is larger, and intracavitary has a large amount of powder dyes point
Secretion, interstitial no inflammation has no obvious fibroplasia；Model group rats lumen of gland reduces, and luminal sectetion is reduced, and interstitial is visible big
Inflammatory cell infiltration is measured, proliferation of fibrous tissue is obvious；Prostat group rat major part prostate lumen of gland is larger, and intracavitary has a large amount of powder
Secretion is contaminated, individual animal secretion is reduced, and interstitial no inflammation has no obvious proliferation of fibrous tissue；Match each dosage of certain herbaceous plants with big flowers extract
The most of prostate lumen of gland of group rat is larger, there is a large amount of powder dye secretion in lumen of gland, and interstitial inflammation substantially mitigates, and has no obvious fibre
Dimensional tissue hyperplasia；Individual animal lumen of gland reduces, and segmental inflammation cellular infiltration, it is seen that hyperplasia that fibr tissue is slight is shown in Fig. 1.
Match influence of the certain herbaceous plants with big flowers extract to prostatitis rat blood serum IL-1 β, TNF-α and IL-2：Certain herbaceous plants with big flowers extract is matched to chronic
IL-1 β and TNF-α in prostatitis rat blood serum have obvious inhibitory action, to IL-1 β and TNF-α during 150mgkg-1
Generation inhibiting rate be respectively 43.88%, 47.49%；The level of IL-2 in serum can be significantly improved simultaneously, during 150mgkg-1
Generation increment rate to IL-2 is 31.19%, and being compared difference with model group has statistical significance, is shown in Table 2.
Table 2 matches certain herbaceous plants with big flowers extract to prostatitis rat blood serum IL-1 β, TNF-α and IL-2 influence
Note:Compared 1) P with blank group<0.01；Compared 2) P with model group<0.05,3) P<0.01.
3.2 match certain herbaceous plants with big flowers extract Carrageenans cause the prostatitic influence match certain herbaceous plants with big flowers extract high dose group of rat acute and mould
Type group compares, and sees that inflammatory cell infiltration degree substantially mitigates in lumen of gland under light microscope, lecithin is close in prostatic fluid
Degree substantially increase, points out match certain herbaceous plants with big flowers extract to have the effect (table 3) of good control prostate acute inflammation.
The prostatitic influence of rat acute of the match certain herbaceous plants with big flowers extract Carrageenan induction of table 3
Note:Compared with blank group*P<0.05；Compared with model group#P<0.05,##P<0.01。
3.3 match certain herbaceous plants with big flowers extract paraxylene cause the influence match high agent of certain herbaceous plants with big flowers extract of mice ear and mouse granuloma induced by implantation of cotton pellets
Amount is compared with blank group, and inflammation has obvious inhibitory action caused by paraxylene；Match certain herbaceous plants with big flowers extract high dose and model group ratio
Compared with to granulomatous formation with significant inhibitory action (table 4).
The match certain herbaceous plants with big flowers extract paraxylene of table 4 causes the influence of ear swelling and granuloma induced by implantation of cotton pellets
Note:Compared with blank group*P<0.05；Compared with model group#P<0.05。
The analgesic activity match certain herbaceous plants with big flowers extract high dose of 3.4 match certain herbaceous plants with big flowers extracts is compared with blank group, and thermostimulation induced pain is tested
In the mouse threshold of pain effect of being significantly improved (table 5).
Table 5 matches influence of the certain herbaceous plants with big flowers extract to thermostimulation
Note:Compared with blank group*P<0.01；Compared with model group#P<0.05。
Influence match certain herbaceous plants with big flowers extract of the 3.5 match certain herbaceous plants with big flowers extracts to Water l oad rat urine volume can substantially increase Water l oad rat 4
The urine volume of period, difference is compared with blank group has statistical significance, points out match certain herbaceous plants with big flowers extract to have significantly Water l oad rat
Diuresis (table 6).
Table 6 matches influence of the certain herbaceous plants with big flowers extract to thermostimulation
Note:Compared with blank group*P<0.05,**P<0.01。
Test example 2 matches the clinical observation of certain herbaceous plants with big flowers extract for treating non-bacterial prostatitis
First, clinical data
1. general information：120 patients are all from outpatient service, are randomly divided into treatment group and each 60 of control group.Wherein treat
Group average age (34.73 ± 8.14) year, the course of disease (2.18 ± 1.61) year, symptom score (20.05 ± 4.60) point, sign scoring
(4.27 ± 1.54) divide；Control group average age (36.71 ± 11.34) year, the course of disease (2.20 ± 1.52) year, symptom score
(19.75 ± 3.98) divide, sign scoring (4.22 ± 1.44) point.Through examine, two groups age, the course of disease and the state of an illness relatively on nothing
Notable difference (every P>0.05), with comparativity.And hyperplasia of prostate, prostate cancer are excluded, do not receive in medical preceding January
Cross other drugs treatment.
2. diagnostic criteria：With reference to NIH's (NIH) prostatitis class definition and《Urology Surgery》System
It is fixed.Patient's prostatic fluid (EPS) leukocyte count>10/HP, the position such as waist, few abdomen, perineum, glans penis under having in various degree
Pain, frequent micturition, urine retention sense, the refreshing tired soreness of waist, impotence and premature ejaculation, and prostate scleroma hardening etc. change, the training of prostatic fluid bacterium
(Meares-Stamey methods) feminine gender is supported, mycoplasma, Chlamydia culture are negative.Tcm diagnosis and exclusion standard are with reference to the Chinese people
What the Ministry of Public Health of republic formulated《New Chinese medicine guideline of clinical investigations》.
2nd, treatment method
1. medicine
Treatment group is decocted in water for oral dose with match certain herbaceous plants with big flowers（General flavone content average out to 41% in terms of rutin in decocting about 2h, water extract）.
The oral Prostat piece of control group (Prostat, Sweden PHARMACIA ALLERGON AB are produced), 2 times a day, and 1 tablet once.Two
Group, for 1 course for the treatment of, continuously carries out therapeutic evaluation with 1 month after 2 courses for the treatment of of treatment.
2. observation item
(1) symptom score：With reference to NIH-CPSI (NIH's Symptom of Chronic Prostatitis) standard
Setting.Dividing pain index, micturition index, the aspect of quality of life index three, totally 9 indexs are scored respectively, 0~21 point of pain index,
0~10 point of micturition index, 0~12 point of quality of life index, 43 points altogether, total mark is higher, and the state of an illness is heavier.
(2) sign scores：Reference《New Chinese medicine guideline of clinical investigations》Record EPS, digital rectal examination and ultrasound diagnosis knot
Really, according to degree it is different with it is light, in, again (1~3 point) represents, be normally 0 point.(being shown in Table 7)
(3) adverse reaction：By checking blood routine, hepatic and renal function, after observation medication, the poison of adverse reaction and medicine is secondary makees
With.
3. statistical method
According to the concrete condition of data, t inspections, rank test is respectively adopted.
3rd, treatment results
1. efficacy assessment standard
With reference to《New Chinese medicine guideline of clinical investigations》Formulate.Recovery from illness:Total score (symptom score+sign scoring) after treatment
For 0；It is effective:Total score declines >=70% after treatment；Effectively:Total score declines between 30%~69% after treatment；It is invalid:After treatment
Total score declines<30%.
2. overall clinical efficacy rate compares
Treatment group fully recovers 14, effective 25, effective 16, invalid 5, total effective rate 91.7%；Control group is fully recovered 6,
Effective 19, effective 22, invalid 13, total effective rate 78.3%.Ridit analysis displays, two groups of total effectses compare P<0.01,
Treatment group's curative effect is better than control group.
3. symptom score compares before and after two groups of treatments
Match certain herbaceous plants with big flowers water decoction has after the treatment of pain index, micturition index, quality of life index and the indices that always grade
It is decreased obviously, and control group ratio, declining degree, there were significant differences.
Match certain herbaceous plants with big flowers group and Prostat group NIH-CPSI scoring changes
Note:Itself compare before and after treatment,*P<0.01；Compared with after control group treatment,ΔP<0.01。
4. sign integral contrast before and after two groups of treatments
As a result show, every integration is decreased obviously after the group treatment of match certain herbaceous plants with big flowers；With control group ratio, integrated and total score in B ultrasound
It is upper that there were significant differences.
Match certain herbaceous plants with big flowers group and the change of Prostat group somatic feature score
Note:Itself compare before and after treatment,*P<0.01；Compared with after control group treatment,ΔP<0.01。
5. toxicity
Two groups of observations are without serious adverse reaction.
Test example 3 matches influence of the certain herbaceous plants with big flowers extract to benign prostatic hyperplasia in rats
1 materials and methods
1.1 animal：Cleaning grade male SD rat 60,210~230g of body weight, Shanghai Slac Experimental Animal Co., Ltd.
There is provided, quality certification number：SCXK2007-0005.
1.2 medicine：50mg/m1 testosterone propionate parenteral solutions（The pharmaceutical factory of Shanghai the 9th, lot number：991203）；Proscar 5mg
（Hangzhou Mo Shadong drugmakers, lot number：BH20020304）；Match certain herbaceous plants with big flowers extract（Embodiment 3 is made）, 50mg/ is configured to respectively
Ml, 100mg/ml, 200mg/ml3 concentration；Testosterone, estradiol radioimmunoassay kit（Beijing North biotechnology research
Institute, lot number：S10940093）.
1.3 instrument：Electronic analytical balance（Shanghai balance equipment factory）；Binocular optical microscope（Japanese Olympus companies）.
1.4 experiment packets and processing
1.4.1 the preparation of hyperplasia of prostate rat animal model：Male SD rat 60, yellow Jackets 40mg/kg abdomens
Chamber injecting anesthetic, through scrotum row aseptic operation, extracts bilateral testes, after recovering 1 week, and every is subcutaneously injected testosterone propionate 4mg/
Kg, continuous 30d, weigh weekly 1 time.
1.4.2 administration and processing：Adaptability is raised 1 week before modeling, is divided into 6 groups, i.e. normal group, model by table of random number
Group, Proscar group, the match basic, normal, high dosage treatment group of certain herbaceous plants with big flowers extract, every group 10.Physiological saline is subcutaneously injected in normal group, remaining
5 groups of hypodermic injection testosterone propionates.Normal group and model group give 10ml/kg physiological saline；Proscar group dosage is 0.8mg/
Kg is dissolved in 10ml/kg physiological saline, and the above uses gastric infusion, and one time a day, continuous 30d.Next day after last gavage, eyeball
Blood sampling is put to death, and peels off prostate surrounding tissue, and electronic analytical balance claims prostate wet weight, takes out prostate siphonal lobe tissue, clip
Identical weight, 10% formalin is fixed.
1.5 observation index and detection method
1.5.1 observation index：Prostata tissue weight in wet base is measured with assay balance；Volumetric method measures volume；Calculate prostate
Index（PI）.PI=prostate wet weight/body weight, body weight before rat body weight-modeling before rat body weight value added=execution.
1.5.2 prostata tissue pathological change：Prostate siphonal lobe same area prostata tissue is taken, 10% formalin is consolidated
It is fixed, routine hematoxylin Yihong（HE）Dyeing, the pathomorphology change of light Microscopic observation prostate section.
1.5.3 biochemical analysis：At the end of administration, eyeball blood sampling, by detection rat blood serum testis the step of kit specification
Ketone, estradiol level.
1.6 statistical procedures：Data are represented with x ± s, are examined using t, with SPSS16.0 statistical softwares processing data
2 results
2.1 experimental result：It is shown in Table 8, table 9.
86 groups of rats of table are put on weight, the comparison of prostate wet weight, volume, index and bulk index
Note：Compared with normal group,**P ＜ 0.01,*P ＜ 0.05；Compared with model group,##P ＜ 0.01,#P ＜ 0.05
The comparison of 96 groups of rat blood serum T, E2 contents of table（x±s）
Note：Compared with normal group,**P ＜ 0.01；Compared with model group,##P ＜ 0.01
2.2 pathologic finding：The arrangement of normal group body of gland is clear, and lumen of gland is without expansion, and galandular epithelium is in simple columnar.Model group body of gland
Dense arrangement, lumen of gland becomes big, part Glandular Dilatation, the obvious hyperplasia of galandular epithelium, and subregion level increase, arrangement disorder is most
Galandular epithelium is dashed forward to intracavitary in mamillary, and interstitial fibers tissue and smooth muscle tissue increase.Preceding Lenin's low dose group is shown in that lumen of gland is obvious
Reduce, tube chamber cell is generally simple columnar arrangement, cell shrinkage, interstitial is few and loose.The middle and high dosage group of certain herbaceous plants with big flowers extract is matched with protecting
Row are controlled a group tube chamber cell list cladding and occurred, and are in high column or cubic epithelial cell, mamilla is significantly reduced more.See
Fig. 2.
Test example 4 matches clinical research of the certain herbaceous plants with big flowers extract to treatment benign prostate hyperplasia
1 data and method
1.1 cases are originated
Patient is andrology of Fujian University of Traditional Chinese Medicine consulting patients in out-patient department, through inquiring medical history, digital rectal examination, ultrasound diagnosis, urine
Flow rate measure etc., which is checked, is diagnosed as good victory hyperplasia of prostate (BPH).Totally 80, it is randomly divided into two groups, treatment group and control group
Each 40.
1.2 diagnostic criteria and case inclusion criteria
1.2.1 inclusive criteria
Western medicine diagnostic criteria was promulgated with reference to Urology Surgery association of Chinese Medical Association in 2006《Benign prostatic hyperplasis is diagnosed
Treatment guidelines》.Tcm diagnosis and CM syndrome differentiation criterion refer to China《New Chinese medicine guideline of clinical investigations) } " Chinese medicine in (1997)
The standard that the good victory hyperplasia of prostate clinical guidance principle of Drugs in Therapy " is formulated, case is demonstrate,proved for kidney deficiency blood addiction selected by this research.It is all
Meet the diagnosis of this disease and Standards of Chinese Medical Syndrome Differentiation, 50~80 years old age male person；Stop relevant administration more than 1 month, examination can be included
Test case.
1.2.2 exclusion standard
(1) urethral calculus, prostate cancer, acute and chronic renal insufficiency, kidney work(（Cr）Abnormal person.(2) neurogenic bladder,
Bladder neoformation, neck of urinary bladder fibrosis, dysuriac caused by ankylurethria.(3) the serious primary disease of hemopoietic system is merged,
Liver function（ALT）More than more than 1 times person of normal value, mental patient；There is hemorrhagic tendency person.(4) allergic constitution person (5) has pelvic cavity
The wounded is damaged in operation.(6) inclusive criteria is not met, not by regulation medication, it is impossible to judge curative effect, or data is not congruent affects the treatment
Or security judgement person.
1.2.4 reject, come off and terminate the standard tested
(1) reject：Group should not be entered but enrolled subject should reject, including：Receive by mistake, mistaken diagnosis, a medicine are unused, nothing
Any detection keeper.The case of rejecting answers illustration, does not make curative effect statistical analysis, but at least receives seance, and has
Safety records person, can participate in analysis of adverse reactions at least one times.(2) come off:Enter group but do not complete the case of clinical protocol,
Following situations should be regarded as coming off, including patient voluntarily exits, lost to follow-up, compliance is poor, concomitant disease makes it exit what is come off by doctor
Case answers illustration, if any baseline efficacy data, and the curative effect index result switching of its last time most can terminate
Fruit carries out statistical analysis.
1.3 medicines, medication, treatment time
（1）Treatment group's medication：Match certain herbaceous plants with big flowers（Embodiment 3 is made）；
（2）Control group medication：The retention of urine relaxes（Produced by Shijiazhuang City Ke Di pharmaceutcal corporation, Ltds）, usage：3 every time, often
Day 2 times.Medicine is constituted：Psoralea corylifolia, edible tulip, Lygodium japonicum, desmodium, amber, motherwort etc. constitute.The course for the treatment of 1 month is 1 treatment
Journey, two groups are treated 1 course for the treatment of.
1.4 observation index
（1）International contest（IPSS）, influence of the urination symptom to quality of life（QOL）；（2）Tcm symptom
Including situations such as enuresis nocturna frequency, dysuria, soreness and weakness of waist and knees, small distention and fullness in the abdomen.（3）Qmax（MFR）Detection（4）Residual urine
Amount（5）Prostate volume
1.5 statistical method
Data analysis is carried out using SPSS11.5 statistics softwares, measurement data is examined using t or F is examined, enumeration data
Using x2Examine.
2 results
Include 60 of this phase experiments are effective case, without coming off or reject.Last effective case 43, wherein controlling
Treatment group 23, control group 20.Meet the quality control requirement in case-control study.In 60 patients, treatment group's age
It is minimum 63 years old, it is maximum 76 years old, it is average（66.54±5.43）Year；The control group age is minimum 52 years old, maximum 80 years old, average（67.49
±4.65）Year.Through examining, P>0.05.Illustrating two groups, there was no significant difference in age distribution.With comparativity.
2.1 total effectses are evaluated
10 two groups of total effectses of table compare
As can be seen from the above table, treatment group's effective percentage is 76.7%；Control group effective percentage is 66.7%.Analyzed through Ridit, P<
0.05, show that match certain herbaceous plants with big flowers clinical efficacy relaxes better than the retention of urine.
2.2IPSS, which scores, and QOL scoring treatments are front and rear changes
Table 11 treats front and rear IPSS scorings and QOL scoring changes
Note:*p<0.05, before treatment after vs treatments;#p<0.05, treatment group's vs control groups.
Examined through t：IPSS and QOL contrast not statistically significant (P with control group before the treatment>0.05).After treatment, controlling
Between treatment group and control group, SPSS compares statistically significant (P<0.05);Then not statistically significant (the P of QOL>0.05).Explanation
Match influence of the certain herbaceous plants with big flowers for improving patient's international contest to relax better than the retention of urine, but match certain herbaceous plants with big flowers can not be illustrated for improving patient
Influence of the symptom of urinating to the scoring of quality of life is relaxed better than the retention of urine.
2.3 tcm symptoms improve situation
Note:With comparing before treating:**P<0.01;With contrast ratio:△P<0.05。
After treatment group and control group treatment, the symptom such as enuresis nocturna frequency, dysuria, soreness and weakness of waist and knees, small distention and fullness in the abdomen has aobvious
Writing improves (P<0.01);Treatment group is compared with control group, improves soreness and weakness of waist and knees, and treatment group is better than control group (P<0.05), change
Kind enuresis nocturna frequency, dysuria, small distention and fullness in the abdomen, two groups of no significant difference (P>0.05).
Compare before and after the treatment of 2.4 Qmaxs
Table 12 is treated front and rear Qmax and compared
Note:*p<0.05, before treatment after vs treatments;#p<0.05, treatment group's vs control groups.
Examined through t:The Qmax of two groups of patients is before the treatment without significant difference (p>0.05).Compare before and after two groups of treatments
Compared with Qmax is significantly increased, statistically significant (p<0.05) two kinds of medicines, are pointed out to improve the maximum urine of patient
Flow rate.Qmax difference comparsion has significant difference (p after being treated between group<0.05), prompting match certain herbaceous plants with big flowers is improving hyperplasia of prostate
Effect in terms of patient's Qmax closes relieving capsule better than addiction.
The change of 2.5 residual urine volumes
Table 13 treats front and rear residual urine volume (mL) change
Note:*p<0.05, before treatment after vs treatments;#p<0.05, treatment group's vs control groups.
Examined through t:Before treatment, two groups of residual urine volumes are without significant difference (p>0.05).Compare before and after two groups of treatments, residual urine
Amount is reduced, statistically significant (p<0.05), two kinds of medicines are pointed out to reduce the residual urine volume of patient.It is residual after being treated between group
Remaining urine volume difference comparsion has significant difference (p<0.05), prompting match certain herbaceous plants with big flowers curative effect in terms of residual urine volume is reduced closes easypro glue better than addiction
Capsule.
The change of 2.6 prostate volumes
Table 14 treats front and rear prostate volume (cm3) change
Note:*p<0.05, before treatment after vs treatments;#p<0.05, treatment group's vs control groups.
Examined through t:Before and after treatment, two groups of prostate volumes are relatively without significant difference (corpse>0.05).Illustrate match certain herbaceous plants with big flowers and addiction
Relieving capsule is closed to fail to reduce the prostate volume of BPH during testing.
2.7 safety monitoring
Before and after 339 Li Sai certain herbaceous plants with big flowers treatment group patients treatment, liver (GPT), kidney (Bun), blood, urine, electrocardiogram, blood pressure, PSA inspections
Look into, have no significant change.
The influence that the match certain herbaceous plants with big flowers extract of test example 5 is bred to prostate gland cancer cell DU-145, PC-3 cell
1 materials and methods
1.1 material
1.1.1 experiment material：
Certain herbaceous plants with big flowers extract is matched, 50mg/ml, 100mg/ml, 200mg/ml3 concentration are configured to respectively）；Cell line：People prostatitis
Gland cancer DU-145 and PC-3 cell line is purchased from Chinese Academy of Sciences's Shanghai Institute of Cell Biology cell bank.
1.1.2 main agents：
HamF12 culture mediums, Gibco companies；RPMI1640 culture mediums, Gibco companies；Hyclone（FBS), Hangzhou four
Ji Qing biological products Co., Ltd；Trypsase, SIGMA companies of the U.S.；Penicillin, streptomysin：Shandong medical share in Shandong has
Limit company；Chloroform etc., Chemical Reagent Co., Ltd., Sinopharm Group；
1.2 method
1.2.1 the preparation of certain herbaceous plants with big flowers extract is matched
Match certain herbaceous plants with big flowers 20g is weighed, is separately added into after 200ml 70% ethanol immersion 30min, heating and refluxing extraction 2 in water-bath
The secondary each 1h of, are filtered while hot, and residue is washed 2~3 times with coordinative solvent, and merging filtrate is reclaimed, and are dried, standby.
1.2.2 the measure of cell proliferation inhibition rate
Detected using mtt assay.Suspension is made in the prostate gland cancer cell in growth period of taking the logarithm, and is seeded to 96 orifice plates, often
The μ L of hole 200, in 5%CO2, 37 DEG C of adherent 4h are then respectively adding enzymolysis polypeptide solution, and each concentration sets 3 parallel holes, simultaneously
If not dosing control group, puts 5%CO2, it is incubated in 37 DEG C of incubators, culture, which terminates to add 180 μ L MTT and 20 μ L PBS, to be continued to train
4h is supported, the liquid for abandoning 96 orifice plates is inhaled, 150 μ L DMSO is added, is sufficiently mixed.Put enzyme-linked immunosorbent assay instrument and survey extinction in 570nm
Degree, calculates cell inhibitory effect index（IR), calculated by following equation.
IR=[(control group A value-medicine group A values)/control group A value] × 100%
1.2.3 data processing
Experimental result is to representData statistic analysis is carried out using SPSS16.0 softwares.
2 results
2.1 match certain herbaceous plants with big flowers extracts are acted on DU-145 and PC-3 cell inhibitory effects
Match certain herbaceous plants with big flowers extract is made into five concentration groups of 5mg/ml, lOmg/ml, 15mg/ml, 20mg/ml and 25mg/ml, point
After not measure effect 24h and 48h, the influence bred to DU-145 and PC-3 cells.As a result such as table 15 below.
The influence that the match certain herbaceous plants with big flowers extract of table 15 is bred to prostate gland cancer cell DU-145, PC-3 cell
Compared with the minimum inhibiting rate of allogenic cell：* represent;P<0.01
As seen from the above table, match certain herbaceous plants with big flowers extract is respectively provided with inhibitory action to DU-145 cells and PC-3 cells, and with concentration with
There is relation action time.And inhibiting rate has respectively reached 82.19% and 81.77% after two kinds of cell 48h of 25mg/ml sample effects.
Test example 6 matches 142 clinical observations of prostate cancer of certain herbaceous plants with big flowers extract for treating
1 clinical data
Observation case be in May, -2012 in January, 2009 the court be in hospital and out-patient totally 142, before pathological diagnosis is
Row gland cancer.It is 18~90 years old age, average 72.1 years old;Pathological diagnosis gland cancer 134, squamous carcinoma 3, small circle cell cancer l is undifferentiated
Cancer 4;Mono- 4NxMIc patients of clinical stages T3 21, wherein merge simultaneously Bone tumour, Lung metastases, pelvic cavity shift extensively (including
Invade bladder, seminal vesicle, psoas, buttocks muscle group etc.) 8, merge Bone tumour, Lung metastases 12, it is single l;The 4NxMlb phases of T3 mono- suffer from
Person 110;Mono- 2N0M0 phases of TI patient 11.Gleason scorings 8-10 points 48,7 points 69,5-6 points 22,2-4 points 3.
The course of disease l-51 months.
2 treatment methods
2.1 western medical treatment references《The Chinese prostate cancer diagnosis treatment guidelines of urological diseases diagnoses and treatment guide one》In
Recommendation therapeutic scheme.It is specific as follows:Tl-2N0M0 phase patient row radical Retropubic Prostatectomies.All patients underwent's all-males
Hormone-blocking treatment, is blocked using maximum endocrine therapy or interval endocrine;Bilateral kingfisher ball resection;And add non-steroid
Antiandrogen medicine Flutamide, each 250mg, 3 times a day, orally;Or Casodex, each 50mg, 1 time a day, orally.Bone turns
Moving ostalgia patient uses isotope radiotherapy, or Zoledronate to treat.
2.2 Chinese traditional treatment:Match certain herbaceous plants with big flowers extract is given, is decocted in water for oral dose（Decocting boils in about 2h, water extract general flavone content with reed
Fourth meter average out to 41%）.
3 observation items and statistical method
3.1 observation items observation patient treatment before and after PSA (PSA) 3, free serum testosterone (FT),
Blood cell analysis, Whole Body Bone Scanning radionuclide scanning (ECT), the situation of change of QLQ-C30 scales, and count overall survival, it is total
Body median survival interval, the overall median time that gets nowhere.
3.2 statistical methods are handled using SPSS16.0 statistical softwares packet, and two samples are used in measurement data analysis
The t that mean compares is examined, enumeration data analysis Chi-square Test.
4 criterions of therapeutical effect and treatment results
4.1QLQ-C30 scale score criterions of therapeutical effect are effective:More than 20 points of increase before standard scores are relatively treated after treatment;Effectively:
10-20 points of standard scores increase;It is unchanged:0-9 points of standard scores increase;Aggravate:Standard scores reduce more than 10 points before relatively treating.
4.2QLQ-C30 scale score curative treatments symptom in December improves effective 115, effective percentage 81.0%;Hot flash symptoms
Incidence 22.4%;Incidence of osteoporosis 13.8%;Mastosis incidence 11.7%.
142 follow-up of patients's 1-50 months of 4.3 survival rates and PSA recurrences, dead 16, overall survival during observation
88.73%；27.7 months Overall median life cycles；Totally get nowhere median time 25.2 months.Wherein 21 patients of T3-4NxMlc phases
Overall progression free survival phase 24.3 months;PSA minimums 0 after castration, PSA recurrence times (the 23.9 soil 19.2) moon, PSA doubling times
(the 24.1 soil 20.4) moon.The overall progression free survival phase of 110 patients of T3-4NxMlb phases 24.3 months;PSA minimums after castration.,
PSA recurrence times (the 20.1 scholar 15.2) moon, (22.3 scholar 17.4) moon PSA doubling times.
4.4 Bone tumour stoves and ECT transfer stove situation T3-4NxMlb phase Bone tumour stoves:Bone tumour stove disappears 4, reduces, becomes
Light 79, no diffusion, nothing increase 18, new transfer stove 9 occur.ECT transfer stoves:It is before the treatment of ECT transfer stoves number
(7.6 scholar 4.7) is individual, and treatment is that (3.4 soil 1.7) is individual after December, individual for (2.6 scholar 1.4) after 24 months, is after 36 months (4.8 scholar 2.1)
It is individual.
Blood cell analysis change, which is compared, before and after 4.5 treatments is shown in Table 16.Treat the 24th, 36 months, leucocyte (WBC) substantially rises
Height, with being compared before treatment, difference is very significant (P<0.01);Treat the 12nd, 24,36 months hemoglobin (Hb), blood
Platelet (BPC) is significantly raised, and with being compared before treatment, difference is very significant (P<0.01).
Table 16 is treated front and rear blood corpuscle parameters and compared
Compared with before treatment, * P<0.01.
Claims (7)
1. purposes of the certain herbaceous plants with big flowers extractive of general flavone in the medicine for preparing treatment hyperplasia of prostate is matched, wherein, described match certain herbaceous plants with big flowers is always yellow
The weight percentage of general flavone is in terms of rutin in ketone extract, no less than 20%.
2. purposes according to claim 1, it is characterised in that：The weight of general flavone in described match certain herbaceous plants with big flowers extractive of general flavone
Percentage composition is in terms of rutin, no less than 55%, and 50% is no less than with myricetin weight.
3. purposes according to claim 1, it is characterised in that：Described match certain herbaceous plants with big flowers extractive of general flavone is by water or organic molten
Agent is extracted and is prepared from.
4. purposes according to claim 3, it is characterised in that：Described organic solvent is more than 70% ethanol.
5. the purposes according to claim 1-4 any one, it is characterised in that：The preparation of the match certain herbaceous plants with big flowers extractive of general flavone
Method comprises the following steps：
A, weigh match certain herbaceous plants with big flowers, add water or organic solvent immersion；
B, using heating water bath refluxing extraction, surname extraction, ultrasonic extraction, Microwave Extraction, SCF-CO 2, impregnate or ooze
Filter extracting method；Residue is washed with coordinative solvent, merging filtrate, filtering, produces match certain herbaceous plants with big flowers extractive of general flavone.
6. purposes according to claim 5, it is characterised in that：Described organic solvent is more than 70% ethanol.
7. purposes according to claim 5, it is characterised in that：Also include step c：Purifying, specific purification process is as follows：
1. after the AB-8 type macroreticular resins that crude drug amount is soaked with ethanol, upper prop is pre-processed, with the ethanol stream of volume fraction 95%
Dynamic cleaning, the ethanol extremely flowed out to ethanol and water 1：Untill the not white muddiness of 5 mixing, then with distilled water flushing to water
Washing lotion is without alcohol taste；
2. the match certain herbaceous plants with big flowers extract solution loading of b step is taken, first with the water elution of 4 times of column volumes, eluent is discarded；After with 4 times of column volumes
10% ethanol elution, discards eluent；Then with 8 times 40% of ethanol elution, eluent is collected, ethanol is recovered under reduced pressure, it is dense
Be reduced to it is dry, produce match certain herbaceous plants with big flowers general flavone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410089748.3A CN103830288B (en) | 2014-03-12 | 2014-03-12 | Match certain herbaceous plants with big flowers extractive of general flavone and its production and use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410089748.3A CN103830288B (en) | 2014-03-12 | 2014-03-12 | Match certain herbaceous plants with big flowers extractive of general flavone and its production and use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103830288A CN103830288A (en) | 2014-06-04 |
| CN103830288B true CN103830288B (en) | 2017-07-25 |
Family
ID=50794391
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410089748.3A Active CN103830288B (en) | 2014-03-12 | 2014-03-12 | Match certain herbaceous plants with big flowers extractive of general flavone and its production and use |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103830288B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106913599A (en) * | 2015-12-26 | 2017-07-04 | 蔡德成 | Maidenhair extractive of general flavone preparation method and applied in resistance prostatitis medicine |
| CN109181852A (en) * | 2018-09-18 | 2019-01-11 | 山东大学 | A kind of Herba Malvastri Coromandeliani volatile oil is preparing the application in anti-curing cancers and antibacterial product |
-
2014
- 2014-03-12 CN CN201410089748.3A patent/CN103830288B/en active Active
Non-Patent Citations (4)
| Title |
|---|
| Pharmacognostic and phytochemical investigation of the leaves of Malvastrum coromandelianum(L.)Garck;Dhirendra B. Sanghai等;《Anc Sci Life》;20130930;第39页第1段，第40页"Phytochemical screening"和"Determination of flavonoid content"部分 * |
| 槲皮素的药理活性与临床应用研究进展;张志琴等;《药学研究》;20130731;第32卷(第7期);第402页左栏第1段 * |
| 芦丁的资源、药理及主要剂型研究进展;李玉山等;《氨基酸和生物资源》;20130331;第35卷(第3期);第14页第2.4节 * |
| 赛葵的解热镇痛抗炎作用;罗谋伦;《中草药》;19990630;第30卷(第6期);第438页第3节 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103830288A (en) | 2014-06-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101357219B (en) | Medicine for treating chronic hepatitis B | |
| CN101862351B (en) | Application of active parts of gallnut in preparing anti-ulcerative colitis medicine | |
| CN104013668B (en) | Licoflavone class extract is used to prepare to be applied in treatment ulcerative colitis medicine | |
| CN102085252B (en) | Pharmaceutical composition for treating ulcerative colitis and colon-targeted micro-pill preparation thereof | |
| CN102225181B (en) | Medicament for treating benign prostatic hyperplasia disease and preparation method thereof | |
| CN102861287A (en) | Chinese medicinal composition for treating damp-heat invasion blood stasis diabetic foot and preparation method thereof | |
| CN103830288B (en) | Match certain herbaceous plants with big flowers extractive of general flavone and its production and use | |
| CN105250821A (en) | Traditional Chinese medicine compound preparation for treating ulcerative colitis and preparation method thereof | |
| CN103041247B (en) | Traditional Chinese medicine suppository for curing colpitis and preparation method thereof | |
| CN103705796B (en) | A kind of pharmaceutical composition for the treatment of diarrhoea and preparation method thereof | |
| CN102858359B (en) | Medicinal composition comprising alcohol-soluble and water-insoluble licorice extract, pharmaceutical preparation, pharmaceutical application, therapeutic method, and preparative method thereof | |
| CN102106993B (en) | Chinese medicinal composition for treating fatty liver and preparation method thereof | |
| CN104147544A (en) | Traditional Chinese medicinal composition for treating chronic anal cryptitis | |
| CN101380434A (en) | Yisheng granules and preparation method thereof | |
| CN101176779B (en) | Preparation method of enema for curing gynecology inflammation | |
| CN103933386B (en) | Compound hemophiliac capsule used for treating hemophilia and preparation method thereof | |
| CN104491101B (en) | Preparing method of traditional Chinese medicine preparation for treatment of chronic renal failure | |
| CN103800745B (en) | A kind of Chinese medicine for the treatment of damp-heat congestion type chronic Pelvic Pain Syndrome | |
| CN106109767A (en) | A kind of compound preparation preventing and treating non-alcohol fatty liver | |
| CN104887766A (en) | Traditional Chinese medicine compound capsules for treating atherosclerosis and preparation method thereof | |
| CN102210725B (en) | Application of hypericum japonicum thunb general flavone in preparing medicament for treating hepatic fibrosis | |
| CN104127545B (en) | New application of murraya tetramera huang and extract thereof in preparation of medicines | |
| CN104491102B (en) | Colon positioning traditional Chinese medicine preparation for treating chronic kidney failure | |
| CN102406869B (en) | Intestine-protecting and detoxifying micro pellets and use thereof | |
| CN104644769A (en) | Application of traditional Chinese medicine composition in preparation of medicine for treating prostatitis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |