CN105055933B - One kind is used for treating cancer Chinese medicine composition and preparation method thereof, preparation and application - Google Patents
One kind is used for treating cancer Chinese medicine composition and preparation method thereof, preparation and application Download PDFInfo
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Abstract
The invention discloses a kind of Chinese medicine composition and preparation method thereof, preparation and application.Described Chinese medicine composition includes 11 ~ 63 parts of 11 ~ 63 parts of polygonum perfoliatum, 9 ~ 58 parts of Chinese lobelia, 4 ~ 52 parts of oldenlandia diffusa, 13 ~ 76 parts of clerodendron yunnanensis Hu leaf, 2 ~ 17 parts of radix dactylicapni root, 18 ~ 73 parts of madder, 4 ~ 52 parts of polygoni cuspidati,radix, 19 ~ 75 parts of rhizoma imperatae, 9 ~ 58 parts of houttuynia cordata root, rough gentian and 5 ~ 57 parts of the selfheal of raw material medicines in portions by weight, is prepared through pre-treatment, extraction, drying.The characteristics of Chinese medicine composition of the present invention has Mutiple Targets, multiaction passage, the advantage for having uniqueness in terms of the anti-mutation of anticancer.Clearly, preparation method science, quality index is clearly controllable, safe, evident in efficacy, has broad application prospects for Chinese medicine composition of the present invention, reasonable recipe, medicine elementary source and agents area.
Description
Technical field
The invention belongs to pharmaceutical technology field, and in particular to a kind of Chinese medicine composition and preparation method thereof, preparation and application.
Background technology
Cancer is the particularly serious disease that the world today endangers human health, and its morbidity and mortality is in rapid increase in recent years
Trend, the exploitation of cancer therapy drug turns into the key subjects of this century new drug research.At present, doctor trained in Western medicine clinical treatment tumour is typically adopted
With surgery excision, radiotherapy, the big treatment method of chemotherapy three, curative effect is quick, but toxicity is big, and great pain is brought to patient.Especially
It is chemical anticarcinogenic drug, often involves normal cell when acting on target cell, some anti-tumor medicine things also usually companion
There is different degrees of mutagenesis genetoxic, cause to add patient while certain tumour is treated and suffer from second tumour
Possibility.In recent years, people are antitumor to Chinese medicine has carried out numerous studies, it is found that Chinese medicine has multicomponent, Mutiple Targets, multichannel
The characteristics of, toxicity is few, small to human injury, the advantage and wide application prospect for having uniqueness in terms of the anti-mutation of anticancer.
Therefore, a kind of few, small to the human injury cancer therapy drug of toxicity is developed to be very important.
The content of the invention
The first object of the present invention is to provide a kind of Chinese medicine composition；Second purpose is to provide the Chinese medicine composition
Preparation method；3rd purpose is the preparation for providing the Chinese medicine composition；4th purpose is to provide the Chinese traditional medicine composition
The application of thing.
The first object of the present invention is achieved in that described Chinese medicine composition includes the polygonum perfoliatum of raw material medicines in portions by weight
11 ~ 63 parts, 9 ~ 58 parts of Chinese lobelia, 4 ~ 52 parts of oldenlandia diffusa, 13 ~ 76 parts of clerodendron yunnanensis Hu leaf, 2 ~ 17 parts of radix dactylicapni root, madder 18
5 ~ 57 parts of ~ 73 parts, 4 ~ 52 parts of polygoni cuspidati,radix, 19 ~ 75 parts of rhizoma imperatae, 9 ~ 58 parts of houttuynia cordata root, 11 ~ 63 parts of rough gentian and selfheal, it is premenstrual
Processing, extraction, drying are prepared.Preferably, the Chinese medicine composition of the present invention, its bulk drug is made up of following Chinese medicine：
18 ~ 52 parts of polygonum perfoliatum, 35 ~ 43 parts of Chinese lobelia, 24 ~ 35 parts of oldenlandia diffusa
31 ~ 51 parts of 5 ~ 15 parts of radix dactylicapni root, 43 ~ 61 parts of madders of clerodendron yunnanensis Hu leaf
13 ~ 34 parts of polygoni cuspidati,radix, 29 ~ 63 parts of rhizoma imperatae, 18 ~ 49 parts of houttuynia cordata root
19 ~ 41 portions of rough gentian, 29 ~ 41 parts of selfheal
As further preferably, Chinese medicine composition of the invention, its bulk drug is made up of following Chinese medicine：
33 parts of polygonum perfoliatum, 35 parts of Chinese lobelia, 29 parts of oldenlandia diffusa
27 parts of 9 parts of radix dactylicapni root, 43 parts of madders of clerodendron yunnanensis Hu leaf
29 parts of polygoni cuspidati,radix, 54 parts of rhizoma imperatae, 34 parts of houttuynia cordata root
33 portions of rough gentian, 29 parts of selfheal
Or：
52 parts of polygonum perfoliatum, 43 parts of Chinese lobelia, 35 parts of oldenlandia diffusa
51 parts of 5 parts of radix dactylicapni root, 54 parts of madders of clerodendron yunnanensis Hu leaf
13 parts of polygoni cuspidati,radix, 63 parts of rhizoma imperatae, 49 parts of houttuynia cordata root
19 portions of rough gentian, 41 parts of selfheal
Or：
18 parts of polygonum perfoliatum, 39 parts of Chinese lobelia, 24 parts of oldenlandia diffusa
31 parts of 15 parts of radix dactylicapni root, 61 parts of madders of clerodendron yunnanensis Hu leaf
34 parts of polygoni cuspidati,radix, 29 parts of rhizoma imperatae, 18 parts of houttuynia cordata root
41 portions of rough gentian, 36 parts of selfheal
The Chinese medicine composition of the present invention, its general flavone content >=2.0%, total saponin content >=1.0%, rosmarinic acid contents >=
0.1%th, madder cellulose content >=0.2%, and tannin, resin and oxalates must not be detected.
The second object of the present invention, which is achieved in that, to be comprised the following steps：
A, radix dactylicapni root, polygoni cuspidati,radix crushed 10 ~ 50 mesh sieves, added acetic acid of the weight than 2 ~ 3 times-alcohol-water mixing molten
Agent, in soaking 4 ~ 8h at 10 ~ 30 DEG C, is transferred in percolator, the ethanol of the mass concentration 30 ~ 70% with medicinal material weight than 5 ~ 7 times
24 ~ 48h of seepage pressure effects, collects percolate, and filtration obtains filtrate a standby；
B, clerodendron yunnanensis Hu leaf, oldenlandia diffusa, houttuynia cordata root, selfheal, Chinese lobelia, madder, polygonum perfoliatum, rough gentian, rhizoma imperatae
Cataclasm is 1 ~ 5cm, and the ethanol for adding mass concentration 50 ~ 95% is extracted, and extract solution filtration obtains filtrate b standby；
C, merging filtrate a and filtrate b, object is obtained in 40 ~ 60 DEG C of vacuum spray dryings.
In above-mentioned preparation method, acetic acid, the matter of second alcohol and water in acetic acid-ethanol-water mixed solvent described in step A
Amount is than being 0.1 ~ 0.5:20~30：69.5~79.9.
In above-mentioned preparation method, the extraction described in step B is to extract in three times, and first time post processing extraction solvent consumption is medicine
8-12 times of material weight, extraction time is 3 hours；Second of post processing extraction solvent consumption is 6-8 times of medicinal material weight, extraction time 2
Hour；Third time post processing extraction solvent consumption is 3-4 times of medicinal material weight, and extraction time is 1 hour.
In above-mentioned preparation method, the temperature control of the extraction described in step B is at 40 ~ 60 DEG C.
The third object of the present invention be achieved in that added in Chinese medicine composition of the present invention it is pharmaceutically acceptable
Auxiliary material, the pharmaceutical dosage form or preparation of clinical receiving is made.The formulation or preparation include but is not limited to capsule, tablet, soft
Capsule, granule, powder, oral liquid formulations, pill, honeyed bolus, sustained release preparation, freeze drying powder injection, paste, cataplasm, patch,
Targeting preparation, controlled release preparation, spray, liniment, tincture etc..
Formulation of the present invention or preparation are preferably capsule, tablet, granule and oral liquid.Capsule can be common hard
Shell capsule or soft capsule, capsulae enterosolubilis, spansule, controlled release capsule, targeting positioning capsule, intelligent capsule etc..Tablet
Can be ordinary tablet or dispersible tablet, enteric coatel tablets, sustained release tablets, controlled release tablet, targeting spacer, artificial intelligence piece etc..Particle
Agent can contain sugar, can also be without sugar.
The fourth object of the present invention is achieved in that application of the Chinese medicine composition in cancer drug is prepared.
It is preferred that, Chinese medicine composition of the present invention is treated in liver-cancer medicine in the application in preparing cancer drug to prepare
Application.
It is preferred that, Chinese medicine composition of the present invention is treated in lung-cancer medicament in the application in preparing cancer drug to prepare
Application.
It is preferred that, Chinese medicine composition of the present invention is treated in gastric cancer medicament in the application in preparing cancer drug to prepare
Application.
The traditional Chinese medical science thinks that heat toxin, hemostasis, phlegm wet are the important factors of induced tumor.Intrinsic heat poison in collaterals of human internal organs, in the stasis of blood
The long-time stimulus of the various pathogenic factors such as blood, phlegm wet, under interaction, with the passing of time body imbalance of yin and yang, pathological product coalescence is then sent out
Raw tumour.Clinically, tumor patient is often accompanied by the scorching hot pain of local lump, heating or dysphoria in chestpalms-soles, thirsty, yellowish fur, just
It is secret, urinate the hot disease such as red.
In Chinese medicine composition of the present invention, Chinese lobelia, oldenlandia diffusa, clearing heat and detoxicating polygonum perfoliatum function, inducing diuresis to remove edema, the scattered stasis of blood
Disappear carbuncle, is monarch drug in a prescription；Madder, giant knotweed root functionality dissipate blood stasis and smoothing collaterals, dehumidifying resolving sputum, clerodendron yunnanensis Hu leaf benefiting qi and raising yang, invigorating the spleen for eliminating dampness, removing toxic substances
The stasis of blood is dissipated, radix dactylicapni root anti-inflammatory analgesic, hemostasis decompression, are altogether ministerial drug；Rough gentian, selfheal, heartleaf houttuynia root functionality heat-clearing and damp-drying drug, clearing liver profit
Courage, purging intense heat radiating, rhizoma imperatae cooling blood and hemostasis, reducing fever and causing diuresis, tonifying Qi of promoting the production of body fluid are allowed to medicine for assistant altogether；Ten a herbs of full side, with knot
For monarch, clearing heat and detoxicating, scattered stasis of blood resolving sputum, benefiting qi and raising yang, the work(of carbuncle that disappears dissipating bind are played altogether, collaterals of human internal organs can be effectively removed
The heat toxin inside contained, changes scattered blood stasis, phlegm wet, improves human immunological competence, therefore have notable therapeutic effect to tumor and cancer.Through existing
Proved for pharmacological experiment study, growth of the Chinese medicine composition of the present invention to tumours such as liver cancer, lung cancer, stomach cancers has significant suppression
Effect.
In the bulk drug of Chinese medicine composition of the present invention, oldenlandia diffusa is complete for the drying of madder wort oldenlandia diffusa
Grass.Summer, the excavation of season in autumn two, remove impurity, clean, dry.This product nature and flavor slight bitter, micro-sweet, are slightly cold, the thoughts of returning home, liver, the spleen channel, function
It is clearing heat and detoxicating, carbuncle that disappears dissipating bind, inducing diuresis for removing edema.It is among the people to be usually used in abscess of throat, dyspnea and cough due to lung-heat, puckery pain of heat gonorrhea, jaundice with damp-heat pathogen, poisonous snake
Bite, the treatment of the hot carbuncle of sore swells.
In the bulk drug of Chinese medicine composition of the present invention, rough gentian is gentianaceae plant Gentiana manshurica, rough gentian, G. triflora or Yunnan
The dry root and rhizome of rough gentian.Spring, the excavation of season in autumn two, cleaning, drying.This product is bitter in taste, trembles with fear, Return liver, gallbladder channel, function heat-clearing are dry
It is wet, purging the liver of pathogenic fire courage fire.Clinic is used under jaundice with damp-heat pathogen, swelling of vulva pruritus vulvae, band, eczema itch, irascibility hot eyes, Hiccough and deaf, hypochondriac pain
Bitter taste, persistent erection, the treatment of the illness such as convulsion.
In the bulk drug of Chinese medicine composition of the present invention, polygonum perfoliatum is the dry aerial parts of polygonaceae plant polygonum perfoliatum.Summer
Tap, dry when blooming.This product nature and flavor acid, is slightly cold, channel tropism, kidney, bladder warp, function inducing diuresis for removing edema are clearing heat and detoxicating, and the scattered stasis of blood is stopped
Blood.Among the people to be used for nephritic dropsy, the infection of the upper respiratory tract, pertussis, rush down dysentery, eczema, furuncle, venomous snake bite is spitted blood, and having blood in stool, it is sick to wait
Disease, effect is notable.
In the bulk drug of Chinese medicine composition of the present invention, houttuynia cordata root is the root of saururaceae plant houttuynia cordata.This product nature and flavor are pungent, micro-
Cold, return lung warp, function is clearing heat and detoxicating, the carbuncle that disappears apocenosis, inducing diuresis for treating strangurtia.Clinic is usually used in lung carbuncle pyemesis, and phlegm heat panting is coughed, hot dysentery, heat
Drench, the treatment of carbuncle sore tumefacting virus.
In the bulk drug of Chinese medicine composition of the present invention, rhizoma imperatae is the dry rhizome of grass cogongrass.Spring, Qiu Erji
Excavation, cleans, dries, and removes fibrous root and film quality leaf sheath, is bundled into wisp.This product nature and flavor are sweet, tremble with fear, return lung, stomach, bladder warp, function
Cooling blood and hemostasis, reducing fever and causing diuresis.It is among the people to be used for blood-head haematemesis, bleeding from five sense organs or subcutaneous tissue, hematuria, pyreticosis polydipsia, jaundice with damp-heat pathogen, oedema oliguria, heat
Drench the diseases such as puckery pain.
In the bulk drug of Chinese medicine composition of the present invention, Chinese lobelia is the drying herb of Campanulaceae Chinese lobelia.Summer adopts
Receive, remove silt, clean, dry.This product nature and flavor are pungent, put down, the thoughts of returning home, small intestine, lung channel, function inducing diuresis to remove edema are clearing heat and detoxicating.It is among the people
It is usually used in extensive abdominal edema, face foot edema, carbuncle swells furunculosis, snake bite and insect sting;The treatment of the diseases such as advanced schistosomiasis ascites.
In the bulk drug of Chinese medicine composition of the present invention, madder is the dry root and rhizome of madder wort madder.Spring, autumn
Two seasons excavated, and removed silt, dried.This product is bitter in taste, trembles with fear, return liver warp, function cool blood, dissolving stasis, and hemostasis is stimulated the menstrual flow.Clinic is main
It is used to spit blood, bleeding from five sense organs or subcutaneous tissue, uterine bleeding, traumatism and bleeding, stasis blocking Amenorrhea, joint pain, the treatment of tumbling and swelling.
In the bulk drug of Chinese medicine composition of the present invention, clerodendron yunnanensis Hu leaf is Yi nationality of Yunnan characteristic medicine, also known as fragrant glorybower root or leaf leaf
（《Yunnan Chinese herbal medicine choosing》）, combined spicebush leaf（《Often selected with Chinese herbal medicine in Kunming》, first recorded in《Sad jail book on Chinese herbal medicine》, it is Verbenaceae Yunnan
The dried leaf of Changshan leaf.Summer, autumn harvesting, cleaning, drying.This product nature and flavor slight bitter, peppery, temperature, Return liver, spleen, kidney channel, function benefit
Gas lift sun, invigorating the spleen for eliminating dampness, dispelling wind and arresting itching.Yunnan is among the people to be usually used under the prolapse of uterus, prolapse of the anus, the enuresis, band, the disease such as pruitus
Disease.
In the bulk drug of Chinese medicine composition of the present invention, radix dactylicapni root is Bai Nationality In Yunnan characteristic medicine, and white language claims " growing hard wheel ",
For the dry root of bloodroot radix dactylicapni, autumn excavation removes impurity, dried.This product is bitter in taste, pungent, cool, function analgesia,
Hemostasis, decompression.Bai nationality is among the people to be usually used in that stomachache, nervous headache, toothache, wound are swollen and ache, traumatism and bleeding, hypertension etc. are controlled
Treat.
In the bulk drug of Chinese medicine composition of the present invention, polygoni cuspidati,radix is the dry rhizome and root of polygonaceae plant giant knotweed.Spring, autumn two
Season excavates, and removes fibrous root, takes advantage of the short disconnected or sheet of fresh-cut, dries.This product nature and flavor slight bitter, is slightly cold, Return liver courage lung channel, function wind-dispelling profit
Wet, the scattered stasis of blood is led to surely, relieving cough and reducing sputum, and clinic is used for joint pain, jaundice with damp-heat pathogen, Amenorrhea, coughing with a lot of sputum, scald, bruise
The diseases such as damage.
In Chinese medicine composition of the present invention, its main ingredient pharmacological component is the compositions such as flavones, saponin(e.Traditional Chinese medicine is carried
Mode is taken to boil extraction for water, many active components are difficult to abundant extraction, and in water boils extraction and drying process, active component
Heated broken ring is serious, and the flavor efficacy of Chinese medicine is lost, and does not reach expected therapeutic effect.The preparation side of Chinese medicine composition of the present invention
Method, is needed for active constituents of medicine and clinical application, is extracted using different extracting methods, solvent and process conditions,
Active component, which is able to fully extract, to be retained, and recovery rate is high, and bioactivity is strong, and objectionable impurities composition is few, Drug safety and is had
Effect property is greatly improved, and anticancer effect is notable.
Experimental study shows that Chinese medicine composition of the present invention is notable not with traditional water extract in terms of chemistry with pharmaceutical component
Together.Present composition general flavone content, total saponin content, madder cellulose content are all remarkably higher than traditional water extract；Of the present invention group
Compound does not detect in tannin, resin and oxalates, but traditional water extract and detects a large amount of tannins, resin and oxalates.Of the present invention group
Dissimilarity of the compound with traditional water extract in terms of chemistry, pharmaceutical component, is pointed out between the two in pharmacological effect, purposes and security
There is dramatically different property in aspect.In addition, emergency toxicology experiment display, Chinese medicine composition LD of the present invention50For 136.82 ± 17.43
~ 191.75 ± 31.87 g/kg, are significantly higher than the LD of traditional water extract50The g/kg of value 65.37 ± 11.36, shows Chinese medicine of the present invention
The security of composition is apparently higher than traditional water extract.Above chemistry and pharmaceutical component research and the equal table of toxicologic study result
Bright, the preparation method of the present composition is very scientific and reasonable.
Chinese medicine composition of the present invention shows that it has obvious inhibiting effect, and energy to Lewis lung cancer through mouse experiment in vivo
Transfer number of the tumour in lungs is substantially reduced, therefore, it can prevent and treat the medicine of lung cancer for preparing.
Chinese medicine composition of the present invention shows that it has to rat liver cancer H22 tumours substantially suppresses to make through mouse experiment in vivo
With therefore, it can prevent and treat the medicine of liver cancer for preparing.
Chinese medicine composition of the present invention shows that it has to the tumours of human gastric cancer SGC- 7901 substantially suppresses to make through experiment in vitro
With therefore, it can prevent and treat the medicine of stomach cancer for preparing.
Chinese medicine composition of the present invention is a multicomponent compound medicine, the characteristics of with Mutiple Targets, multiaction passage, in anticancer
There is the advantage of uniqueness in terms of anti-mutation.Chinese medicine composition of the present invention, reasonable recipe, medicine elementary source and agents area clearly, are prepared
Methodological science, quality index is clearly controllable, safe, evident in efficacy, has broad application prospects.
Embodiment
With reference to embodiment, the present invention is further illustrated, but the present invention is not any limitation as in any way,
Based on present invention teach that any conversion or replacement made, belong to protection scope of the present invention.
Chinese medicine composition of the present invention, including 11 ~ 63 parts of the polygonum perfoliatum of raw material medicines in portions by weight, 9 ~ 58 parts of Chinese lobelia,
It is 4 ~ 52 parts of oldenlandia diffusa, 13 ~ 76 parts of clerodendron yunnanensis Hu leaf, 2 ~ 17 parts of radix dactylicapni root, 18 ~ 73 parts of madder, 4 ~ 52 parts of polygoni cuspidati,radix, white
5 ~ 57 parts of 19 ~ 75 parts of lalang grass rhizome, 9 ~ 58 parts of houttuynia cordata root, 11 ~ 63 parts of rough gentian and selfheal, are prepared into through pre-treatment, extraction, drying
Arrive.
Polygonum perfoliatum 18 ~ 52 part of the described bulk drug including parts by weight, 35 ~ 43 parts of Chinese lobelia, oldenlandia diffusa 24 ~ 35
Part, 31 ~ 51 parts of clerodendron yunnanensis Hu leaf, 5 ~ 15 parts of radix dactylicapni root, 43 ~ 61 parts of madder, 13 ~ 34 parts of polygoni cuspidati,radix, 29 ~ 63 parts of rhizoma imperatae,
29 ~ 41 parts of 18 ~ 49 parts of houttuynia cordata root, 19 ~ 41 parts of rough gentian and selfheal.
Described bulk drug includes 33 parts of polygonum perfoliatum, 35 parts of Chinese lobelia, 29 parts of oldenlandia diffusa, the clerodendron yunnanensis Hu leaf of parts by weight
40 parts, 9 parts of radix dactylicapni root, 43 parts of madder, 29 parts of polygoni cuspidati,radix, 54 parts of rhizoma imperatae, 34 parts of houttuynia cordata root, 33 parts of rough gentian and selfheal
29 parts.
Described bulk drug includes 52 parts of polygonum perfoliatum, 43 parts of Chinese lobelia, 35 parts of oldenlandia diffusa, the clerodendron yunnanensis Hu leaf of parts by weight
51 parts, 5 parts of radix dactylicapni root, 54 parts of madder, 13 parts of polygoni cuspidati,radix, 63 parts of rhizoma imperatae, 49 parts of houttuynia cordata root, 19 parts of rough gentian and selfheal
41 parts.
Described bulk drug includes 18 parts of polygonum perfoliatum, 39 parts of Chinese lobelia, 24 parts of oldenlandia diffusa, the clerodendron yunnanensis Hu leaf of parts by weight
31 parts, 15 parts of radix dactylicapni root, 61 parts of madder, 34 parts of polygoni cuspidati,radix, 29 parts of rhizoma imperatae, 18 parts of houttuynia cordata root, 41 parts of rough gentian and summer are withered
36 parts of grass.
General flavone content >=2.0% in described Chinese medicine composition, total saponin content >=1.0%, rosmarinic acid contents >=
0.1%, madder cellulose content >=0.2%, and tannin, resin and oxalates must not be detected.
The preparation method of Chinese medicine composition of the present invention, comprises the following steps：
A, radix dactylicapni root, polygoni cuspidati,radix crushed 10 ~ 50 mesh sieves, added acetic acid of the weight than 2 ~ 3 times-alcohol-water mixing molten
Agent, in soaking 4 ~ 8h at 10 ~ 30 DEG C, is transferred in percolator, the ethanol of the mass concentration 30 ~ 70% with medicinal material weight than 5 ~ 7 times
24 ~ 48h of seepage pressure effects, collects percolate, and filtration obtains filtrate a standby；
B, clerodendron yunnanensis Hu leaf, oldenlandia diffusa, houttuynia cordata root, selfheal, Chinese lobelia, madder, polygonum perfoliatum, rough gentian, rhizoma imperatae
Cataclasm is 1 ~ 5cm, and the ethanol for adding mass concentration 50 ~ 95% is extracted, and extract solution filtration obtains filtrate b standby；
C, merging filtrate a and filtrate b, object is obtained in 40 ~ 60 DEG C of vacuum spray dryings.
Acetic acid, the mass ratio of second alcohol and water are 0.1 ~ 0.5 in acetic acid-ethanol-water mixed solvent described in step A:20~
30：69.5~79.9.
Extraction described in step B is to extract in three times, and first time post processing extraction solvent consumption is 8-12 times of medicinal material weight, is carried
It is 3 hours to take the time；Second of post processing extraction solvent consumption is 6-8 times of medicinal material weight, 2 hours extraction times；Third time extracts molten
Agent consumption is 3-4 times of medicinal material weight, and extraction time is 1 hour.
The temperature control of extraction described in step B is at 40 ~ 60 DEG C.
The preparation of Chinese medicine composition of the present invention is to add pharmaceutically receive in described Chinese medicine composition
Auxiliary material be made, including powder, tablet, capsule, granule, pill, oral liquid or paste etc..
The application of Chinese medicine composition of the present invention is described Chinese medicine composition in treating cancer medicine is prepared
Using.
Application of the described Chinese medicine composition in treatment liver cancer, lung cancer and gastric cancer medicament is prepared.
The preparation of the Chinese medicine composition 1 of embodiment 1
1. medicine elementary source and the place of production
Oldenlandia diffusa：For the drying herb of madder wort oldenlandia diffusa, the place of production is Baoshan, Yunnan, by Yunnan Province one
Xin Tang Chinese herbal medicines Co., Ltd provides.
Houttuynia cordata root：For the dry aerial parts of saururaceae plant houttuynia cordata, the place of production is Kunming, Yunnan, by Yunnan Province's wholeheartedly hall
Chinese herbal medicine Co., Ltd provides.
Rhizoma imperatae：For the dry rhizome of grass cogongrass, the place of production is Dongzhou Period in Chuxiong, by Yunnan Province's wholeheartedly hall Chinese herbal medicine
Co., Ltd provides.
Rough gentian：For the dry root and rhizome of gentianaceae plant Yunnan rough gentian, the place of production is Lincang, in Yunnan Province wholeheartedly hall
Herbal medicine Co., Ltd provides.
Chinese lobelia：For the drying herb of Campanulaceae Chinese lobelia.June 15 in 2008 is harvested in Kunming, Yunnan Yiliang County,
Crude drug is accredited as Campanulaceae Chinese lobelia through Kunming drug research institute of pharmacy group Zhou Rongguang senior engineers.
Polygonum perfoliatum：For the dry aerial parts of polygonaceae plant polygonum perfoliatum.On July 26th, 2008 is harvested in Yunnan Province Qujing land
Good county, crude drug is accredited as polygonaceae plant polygonum perfoliatum through Kunming pharmacy group drug research Zhou Rongguang institute senior engineers.
Madder：For the dry root and rhizome of madder wort madder.The place of production is Henan, by Yunnan Province's wholeheartedly hall Chinese herbal medicine
Co., Ltd provides.
Clerodendron yunnanensis Hu leaf：For the dried leaf of Verbenaceae clerodendron yunnanensis Hu.On October 12nd, 2008 is harvested in Yunnan Province Zhaotong
Daguan County, it is normal that crude drug is accredited as Verbenaceae Yunnan through Kunming drug research institute of pharmacy group Zhou Rongguang senior engineers
Mountain.
Radix dactylicapni root：For the dry root of bloodroot radix dactylicapni.September is harvested in Dali Wei Shan on the 18th within 2008,
Crude drug is accredited as bloodroot radix dactylicapni root through Kunming drug research institute of pharmacy group Zhou Rongguang senior engineers.
2. prescription
33 parts of polygonum perfoliatum, 35 parts of Chinese lobelia, 29 parts of oldenlandia diffusa
40 parts of 9 parts of radix dactylicapni root, 43 parts of madders of clerodendron yunnanensis Hu leaf
29 parts of polygoni cuspidati,radix, 54 parts of rhizoma imperatae, 34 parts of houttuynia cordata root
33 portions of rough gentian, 29 parts of selfheal
3. preparation method：
Step 1）：Radix dactylicapni root, polygoni cuspidati,radix are weighed by recipe quantity, 10 sieve meshes were crushed, the second of 3 times of amounts of medicinal material weight is added
Acid-ethanol-water mixed solvent（0.1:20:79.9, m/m/m）, it is closed to be standing and soak for 8 hours, it is fitted into percolator, uses medicinal material
The mass concentration of 5 times of amounts of weight extracts 24h for 70% ethanol percolation, collects percolate, and filtration obtains filtrate A standby；
Step 2）：Clerodendron yunnanensis Hu leaf, oldenlandia diffusa, houttuynia cordata root, selfheal, Chinese lobelia, madder, thick stick are weighed by recipe quantity
Plate is returned, rough gentian, rhizoma imperatae, the cataclasm short section for 1-5cm, is extracted in three times for 50% ethanol with mass concentration.Extract for the first time
Solvent load is 8 times of medicinal material weight, and extraction time is 3 hours；Second of post processing extraction solvent consumption is 6 times of medicinal material weight, is carried
Take time 2 h；Third time post processing extraction solvent consumption is 3 times of medicinal material weight, and extraction time is 1 hour.Extracting temperature control exists
40-60℃.After extraction is finished, extract solution filtration obtains liquor B standby；
Step 3）：Merging filtrate A and liquor B, in 40-60 DEG C of vacuum spray drying into powdered, are produced.
The preparation of the Chinese medicine composition 2 of embodiment 2
1. medicine elementary source and the place of production
Be the same as Example 1.
2. prescription
52 parts of polygonum perfoliatum, 43 parts of Chinese lobelia, 35 parts of oldenlandia diffusa
51 parts of 5 parts of radix dactylicapni root, 54 parts of madders of clerodendron yunnanensis Hu leaf leaf
13 parts of polygoni cuspidati,radix, 63 parts of rhizoma imperatae, 49 parts of houttuynia cordata root
19 portions of rough gentian, 41 parts of selfheal
3. preparation method：
Step 1）：Radix dactylicapni root, polygoni cuspidati,radix are weighed by recipe quantity, 50 sieve meshes were crushed, the second of 2 times of amounts of medicinal material weight is added
Acid-ethanol-water mixed solvent（0.5:30:69.5, m/m/m）, it is closed to be standing and soak for 4 hours, it is fitted into percolator, uses medicinal material
The mass concentration of 7 times of amounts of weight extracts 48h for 30% ethanol percolation, collects percolate, and filtration obtains filtrate A standby；
Step 2）：By recipe quantity weigh clerodendron yunnanensis Hu leaf, oldenlandia diffusa, houttuynia cordata root, selfheal, Chinese lobelia, madder,
Polygonum perfoliatum, rough gentian, rhizoma imperatae, the cataclasm short section for 1-5cm are extracted in three times with mass concentration for 95% ethanol.Carry for the first time
It is 12 times of medicinal material weight to take solvent load, and extraction time is 3 hours；Second of post processing extraction solvent consumption is 8 times of medicinal material weight,
2 hours extraction times；Third time post processing extraction solvent consumption is 4 times of medicinal material weight, and extraction time is 1 hour.Extracting temperature is controlled
At 40-60 DEG C.After extraction is finished, extract solution filtration obtains liquor B standby；
Step 3）：Merging filtrate A and liquor B, in 40-60 DEG C of vacuum spray drying into powdered, are produced.
The preparation of the Chinese medicine composition 3 of embodiment 3
1. medicine elementary source and the place of production
Be the same as Example 1.
2. prescription
18 parts of polygonum perfoliatum, 39 parts of Chinese lobelia, 24 parts of oldenlandia diffusa
31 parts of 15 parts of radix dactylicapni root, 61 parts of madders of clerodendron yunnanensis Hu leaf leaf
34 parts of polygoni cuspidati,radix, 29 parts of rhizoma imperatae, 18 parts of houttuynia cordata root
41 portions of rough gentian, 36 parts of selfheal
3. preparation method：
Step 1）：Radix dactylicapni root, polygoni cuspidati,radix are weighed by recipe quantity, 10 sieve meshes were crushed, 1.5 times of amounts of medicinal material weight are added
Acetic acid-ethanol-water mixed solvent（0.2:25:74.8, m/m/m）, it is closed to be standing and soak for 5 hours, it is fitted into percolator, medication
The mass concentration of 6 times of amounts of material weight extracts 36h for 50% ethanol percolation, collects percolate, and filtration obtains filtrate A standby；
Step 2）：By recipe quantity weigh clerodendron yunnanensis Hu leaf, oldenlandia diffusa, houttuynia cordata root, selfheal, Chinese lobelia, madder,
Polygonum perfoliatum, rough gentian, rhizoma imperatae, the cataclasm short section for 1-5cm are extracted in three times with mass concentration for 75% ethanol.Carry for the first time
It is 10 times of medicinal material weight to take solvent load, and extraction time is 3 hours；Second of post processing extraction solvent consumption is 7 times of medicinal material weight,
2 hours extraction times；Third time post processing extraction solvent consumption is 3 times of medicinal material weight, and extraction time is 1 hour.Extracting temperature is controlled
At 40-60 DEG C.After extraction is finished, extract solution filtration obtains liquor B standby；
Step 3）：Merging filtrate A and liquor B, in 40-60 DEG C of vacuum spray drying into powdered, are produced.In embodiment 4
The preparation of drug composition 4
1. medicine elementary source and the place of production
Be the same as Example 1.
2. prescription
11 parts of polygonum perfoliatum, 9 parts of Chinese lobelia, 52 parts of oldenlandia diffusa
76 parts of 2 parts of radix dactylicapni root, 73 parts of madders of clerodendron yunnanensis Hu leaf leaf
52 parts of polygoni cuspidati,radix, 19 parts of rhizoma imperatae, 58 parts of houttuynia cordata root
11 portions of rough gentian, 5 parts of selfheal
3. preparation method：
Step 1）：Radix dactylicapni root, polygoni cuspidati,radix are weighed by recipe quantity, 30 sieve meshes were crushed, the second of 2 times of amounts of medicinal material weight is added
Acid-ethanol-water mixed solvent（0.3:22.5:77.2, m/m/m）, it is closed to be standing and soak for 6 hours, it is fitted into percolator, medication
The mass concentration of 6.5 times of amounts of material weight extracts 36h for 55% ethanol percolation, collects percolate, and filtration obtains filtrate A standby
With；
Step 2）：By recipe quantity weigh clerodendron yunnanensis Hu leaf, oldenlandia diffusa, houttuynia cordata root, selfheal, Chinese lobelia, madder,
Polygonum perfoliatum, rough gentian, rhizoma imperatae, the cataclasm short section for 1-5cm are extracted in three times with mass concentration for 65% ethanol.Carry for the first time
It is 11 times of medicinal material weight to take solvent load, and extraction time is 3 hours；Second of post processing extraction solvent consumption is the 7.5 of medicinal material weight
Times, 2 hours extraction times；Third time post processing extraction solvent consumption is 3.5 times of medicinal material weight, and extraction time is 1 hour.Extracting temperature
Control is at 40-45 DEG C.After extraction is finished, extract solution filtration obtains liquor B standby；
Step 3）：Merging filtrate A and liquor B, in 40-45 DEG C of vacuum spray drying into powdered, are produced.In embodiment 5
The preparation of drug composition 5
1. medicine elementary source and the place of production
Be the same as Example 1.
2. prescription
63 parts of polygonum perfoliatum, 58 parts of Chinese lobelia, 4 parts of oldenlandia diffusa
13 parts of 17 parts of radix dactylicapni root, 18 parts of madders of clerodendron yunnanensis Hu leaf leaf
4 parts of polygoni cuspidati,radix, 75 parts of rhizoma imperatae, 9 parts of houttuynia cordata root
63 portions of rough gentian, 57 parts of selfheal
3. preparation method：
Step 1）：Radix dactylicapni root, polygoni cuspidati,radix are weighed by recipe quantity, 20 sieve meshes were crushed, 2.5 times of amounts of medicinal material weight are added
Acetic acid-ethanol-water mixed solvent（0.5:20:79.5, m/m/m）, it is closed to be standing and soak for 4 hours, it is fitted into percolator, medication
The mass concentration of 7 times of amounts of material weight extracts 45h for 70% ethanol percolation, collects percolate, and filtration obtains filtrate A standby；
Step 2）：By recipe quantity weigh clerodendron yunnanensis Hu leaf, oldenlandia diffusa, houttuynia cordata root, selfheal, Chinese lobelia, madder,
Polygonum perfoliatum, rough gentian, rhizoma imperatae, the cataclasm short section for 1-5cm are extracted in three times with mass concentration for 95% ethanol.Carry for the first time
It is 10 times of medicinal material weight to take solvent load, and extraction time is 3 hours；Second of post processing extraction solvent consumption is 8 times of medicinal material weight,
2 hours extraction times；Third time post processing extraction solvent consumption is 3 times of medicinal material weight, and extraction time is 1 hour.Extracting temperature is controlled
At 55-60 DEG C.After extraction is finished, extract solution filtration obtains liquor B standby；
Step 3）：Merging filtrate A and liquor B, in 55-60 DEG C of vacuum spray drying into powdered, are produced.Embodiment 6 into
Divide research
Chinese medicine composition 1 of the present invention：Referred to as " composition 1 " is prepared by the embodiment of the present invention 1；
Chinese medicine composition 2 of the present invention：Referred to as " composition 2 " is prepared by the embodiment of the present invention 2；
Chinese medicine composition 3 of the present invention：Referred to as " composition 3 " is prepared by the embodiment of the present invention 3；
Chinese medicine composition 4 of the present invention：Referred to as " composition 4 " is prepared by the embodiment of the present invention 4；
Chinese medicine composition 5 of the present invention：Referred to as " composition 5 " is prepared by the embodiment of the present invention 5；
Traditional water extract control sample：Referred to as " traditional water extract ", preparation method is：Weigh the parts by weight of polygonum perfoliatum 33, half
The parts by weight of side lotus 35, the parts by weight of oldenlandia diffusa 29, the parts by weight of clerodendron yunnanensis Hu leaf 40, the parts by weight of radix dactylicapni root 9, the weight of madder 43
Part, the parts by weight of polygoni cuspidati,radix 29, the parts by weight of rhizoma imperatae 54, the parts by weight of houttuynia cordata root 34, the parts by weight of rough gentian 33, the parts by weight of selfheal 29,
Add water to cook it is secondary, 1 hour every time, collecting decoction, filtration, filtrate concentration, be evaporated, produce.
2. laboratory apparatus and reagent
Agilent1200 high performance liquid chromatographs（Agilent company of the U.S.）；UV-2700 ultraviolet specrophotometers（Japan
Shimadzu Corporation）；B204-N type electronic balances（Mettler companies of Switzerland）.Control substance of Rutin, oleanolic acid reference substance, rosemary
Sour reference substance, rubimaillin reference substance, purpurin reference substance are provided by Products in China inspection institute；Other reagents are equal
Pure to analyze, water is deionized water.
3. experimental method
The measure of 3.1 general flavone contents
The preparation of reference substance solution：It is appropriate that precision weighs the control substance of Rutin being dried under reduced pressure at 120 DEG C to constant weight, plus 60%
Solution of every 1ml containing 0.1mg is made in ethanol, produces.
The preparation of need testing solution：Precision weighs test sample 10mg, puts in 50ml measuring bottles, plus 60% ethanol is diluted to quarter
Degree, shakes up, produces.
Determination method：Precision draws reference substance solution and each 5ml of need testing solution, puts respectively in 10ml measuring bottles, respectively adds 5%
Sodium nitrite solution 0.3ml, shakes up, and places 6 minutes.10% aluminum nitrate solution 0.3ml is separately added into, is shaken up, is placed 6 minutes, then
1mol/L sodium hydroxide solution 4ml are added, scale is diluted to 60% ethanol, shakes up, are placed 10 minutes.Take test sample molten simultaneously
Liquid 5ml, plus 60% ethanol are diluted to 10ml, are used as blank.According to AAS（Chinese Pharmacopoeia one annex V of version in 2010
B）, trap is determined at 505nm wavelength, correction is subtracted, calculates, produces.This product general flavone content is with anhydrous rutin
（C27H30O16）Meter.It the results are shown in Table 1.
The assay of 3.2 total saposins
The preparation of reference substance solution：Precision weighs the mg of oleanolic acid reference substance 5.0, is dissolved with methanol and is settled to 2
ML, shakes up, and obtains the oleanolic acid standard solution that concentration is 2.5 mg/mL.
The preparation of need testing solution：Precision weighs test sample 10mg, puts in 50ml measuring bottles, plus methanol dilution is to scale,
Shake up, produce.
Determination method：Precision draws reference substance solution and each 5ml of need testing solution, puts respectively in 10ml measuring bottles, 70 DEG C of water-baths
On be evaporated or dry up, in each test tube plus the 5 % vanillic aldehydes-mL of glacial acetic acid solution 0.2, the mL of perchloric acid 0.8 that newly prepare,
Put and 15 min are heated in 60 DEG C of water-baths, ice-water bath cooling, the mL of acetic acid 5 on the rocks shakes up, and places 10 minutes.Take simultaneously for trying
Product solution 5ml, plus 60% ethanol are diluted to 10ml, are used as blank.According to AAS（Chinese Pharmacopoeia one annex of version in 2010
Ⅴ B）, trap is determined at 560nm wavelength, correction is subtracted, calculates, produces.This product total saponin content is with oleanolic acid
（C30H48O3）Meter.It the results are shown in Table 1.
3.3 rosmarinic acid contents are determined
Chromatographic condition and system suitability:Using octadecylsilane chemically bonded silica as filler；With methanol -0.1%
Trifluoroacetic acid solution (42:58) it is mobile phase；Detection wavelength is 330nm.Number of theoretical plate is calculated by Rosmarinic acid peak to be not less than
6000。
The preparation of reference substance solution:Take Rosmarinic acid reference substance appropriate, it is accurately weighed, plus Diluted Alcohol is made every 1m1 and contains
0.5mg solution, is produced.
The preparation of need testing solution:Test sample 0.5g is taken, it is accurately weighed, put in conical flask with cover, precision adds dilute second
Alcohol 50ml, ultrasonically treated (power 90W, frequency 59kHz) 30 minutes is let cool, then weighed weight, and the weight of less loss is supplied with Diluted Alcohol
Amount, shakes up, and filters, takes subsequent filtrate, produce.
Determination method：It is accurate respectively to draw reference substance solution and each 5ul of need testing solution, liquid chromatograph is injected, is determined, i.e.,
.
This product rosmarinic acid contents are with Rosmarinic acid（C18H16O8）Meter.It the results are shown in Table 1.
The measure of 3.4 madder cellulose contents
Chromatographic condition and system suitability：Using octadecylsilane chemically bonded silica as filler；With methanol-acetonitrile
0.2% phosphoric acid solution (25:50:25) it is mobile phase；Detection wavelength is 250nm.Number of theoretical plate presses rubimaillin, hydroxyl madder
Plain peak, which is calculated, all should be not less than 4000.
The preparation of reference substance solution：Take rubimaillin reference substance, purpurin reference substance appropriate, it is accurately weighed, plus
Every 1ml 0.1mg containing rubimaillin, the μ g of hydroxyl rubican 40 solution is respectively prepared in methanol, produces.
The preparation of need testing solution：Test sample 0.5g is taken, it is accurately weighed, put in conical flask with cover, precision adds methanol
100ml, close plug, weighed weight is stood overnight, ultrasonically treated (power 250W, frequency 40kHz) 30 minutes, is let cool, then weighed heavy
Amount, the weight of less loss is supplied with methanol, is shaken up, and is filtered, and precision measures subsequent filtrate 50ml, is evaporated, and residue adds the hydrochloric acid of methanol 25%
(4：1) mixed solution 20ml dissolves, and puts heating hydrolysis 30 minutes in water-bath, cools down immediately, add triethylamine 3ml, mix, transfer
Into 25m1 measuring bottles, plus methanol is to scale, shakes up, filtration, takes subsequent filtrate, produces.
Determination method：It is accurate respectively to draw the μ l of the reference substance solution 10 and μ l of need testing solution 20, liquid chromatograph is injected, is surveyed
It is fixed, produce.
This product madder cellulose content is with rubimaillin (C17H15O4) and purpurin (C14H8O5) calculate.
The inspection of 3.5 tannins
The mg of test sample 1, plus the physiological saline 5ml containing 1% egg newly prepared are taken, after mixing, is placed 10 minutes.If
There is muddy or precipitation, then it represents that have tannin detection；Otherwise, it means that without tannin detection.
The inspection of 3.6 resins
The mg of test sample 5 is taken, chlorination imitates 10ml, and shaking is extracted, and is divided and is taken chloroform solution, is evaporated, residue acetic acid 2ml on the rocks makes
Dissolving, puts in tool plug test tube, add water 3ml, mixes, and places 30 minutes.If there is floccule precipitation, resin detection is indicated；Conversely,
Then indicate no resin detection.
The inspection of 3.7 oxalates
The mg of test sample 2 is taken, according to《Chinese Pharmacopoeia》The S methods of one annex of version in 2010 Ⅸ are checked.If occurring muddy or heavy
Form sediment, then it represents that have oxalates detection；Otherwise, it means that without oxalates detection.
The quality measurements of table 1
| Item No. | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| Detection project | General flavone (%) | Total saposins (%) | Rosmarinic acid (%) | Madder element (%) | Tannin | Resin | Oxalates |
| Traditional water is extracted | 0.15 | 0.52 | 0.24 | 0 | Detection | Detection | Detection |
| Composition 1 | 6.18 | 5.98 | 0.28 | 0.51 | Do not detect | Do not detect | Do not detect |
| Composition 2 | 7.14 | 4.39 | 0.44 | 0.69 | Do not detect | Do not detect | Do not detect |
| Composition 3 | 5.49 | 5.77 | 0.31 | 0.72 | Do not detect | Do not detect | Do not detect |
| Composition 4 | 4.37 | 5.43 | 0.12 | 0.85 | Do not detect | Do not detect | Do not detect |
| Composition 5 | 5.43 | 6.11 | 0.69 | 0.21 | Do not detect | Do not detect | Do not detect |
It is above-mentioned test result indicates that, the present composition chemistry with pharmaceutical component in terms of with traditional water extract significantly not
Together：Present composition general flavone content, total saponin content, madder cellulose content are all remarkably higher than traditional water extract；Of the present invention group
Compound does not detect in tannin, resin and oxalates, but traditional water extract and detects a large amount of tannins, resin and oxalates.Of the present invention group
Dissimilarity of the compound with traditional water extract in terms of chemistry, pharmaceutical component, is pointed out between the two in pharmacological effect, purposes and security
There is dramatically different property in aspect.
The safety experiment of embodiment 7（Acute toxicity testing）
1. test sample
Chinese medicine composition 1, Chinese medicine composition 2, Chinese medicine composition 3, Chinese medicine composition 4, Chinese medicine composition 5, traditional water extraction
Thing control sample：Be the same as Example 6.
2. experimental animal
Kunming mouse, body weight 18-22g, male, Kunming Medical University's Experimental Animal Center is provided.
3rd, experimental method
By experiment mice 120,6 groups are equally divided into：1 group of composition, 2 groups of composition, 3 groups of composition, composition 4, group
Compound 5 and traditional water extract group, every group 20.Dosage is allocated by Karber methods, is observed 72 hours after gavage, record
Every group of animal dead number, calculates LD50。
4th, experimental result：
Each experimental group LD50Testing result is shown in Table 2.
The LD50 testing results of table 2
| Sequence number | Group | LD50（g/kg） |
| 1 | Composition 1 | 187.81±24.93 |
| 2 | Composition 2 | 191.75±31.8 |
| 3 | Composition 3 | 165.44±19.58 |
| 4 | Composition 4 | 136.82±17.43 |
| 5 | Composition 5 | 142.95±17.21 |
| 4 | Traditional water extract | 65.37±11.36 |
Experiment display, Chinese medicine composition LD of the present invention50It is significantly high for 136.82 ± 17.43 ~ 191.75 ± 31.87 g/kg
In the LD of traditional water extract50The g/kg of value 65.37 ± 11.36, this shows, the security of Chinese medicine composition of the present invention apparently higher than
Traditional water extract.
Inhibitory action research of the embodiment 8 to mice lung cancer Lewis tumours
1. test sample
Chinese medicine composition 1 of the present invention：Referred to as " composition 1 " is prepared by the embodiment of the present invention 1；
Chinese medicine composition 2 of the present invention：Referred to as " composition 2 " is prepared by the embodiment of the present invention 2；
Chinese medicine composition 3 of the present invention：Referred to as " composition 3 " is prepared by the embodiment of the present invention 3；
Chinese medicine composition 4 of the present invention：Referred to as " composition 4 " is prepared by the embodiment of the present invention 4；
Chinese medicine composition 5 of the present invention：Referred to as " composition 5 " is prepared by the embodiment of the present invention 5；
Control drug fluorouracil：The upper good fortune pharmaceutcal corporation, Ltd of Hisense's friendship nine provides, code H31021009
2. laboratory apparatus and reagent
CHD types light microscope (Japanese Olympus companies)；B204-N type electronic balances（Mettler companies of Switzerland）.
Lewis lung cancer cell lines, are provided by China Concord Medical Science University's cell centre immunization experiment room.
3. experimental animal
C57BL/6J inbred mouses, 6 weeks mouse ages, body weight 22-24 g, Kunming Medical University's Experimental Animal Center is provided.
4. experimental method
After experiment mice adaptability is fed 1 week, in every mouse subcutaneous injection culture Lewis lung cancer cell line 0.2
Divide 7 groups at random by successful 70 mouse of modeling after mL, 5d：Model group（Equivalent distilled water）, 1 group of composition (10mg/kg),
2 groups of composition（10mg/kg), 3 groups of composition（10mg/kg), 4 groups of composition（10mg/kg), 5 groups of composition（10mg/kg) and
Positive control medicine fluorouracil group (10mg/kg), every group 10.Each medicine group presses prescribed dose gastric infusion, and model group is given
Equivalent distilled water is given, 1 time a day, successive administration three weeks.In 8h after last dose, tumor mass is separated, is weighed, calculate tumour inhibiting rate.
Chorista, is weighed, is fixed in 4% paraformaldehyde simultaneously, and observation counts the number of mouse lung tissue Nodules.
Tumour inhibiting rate（%）=（Model group knurl weight-medicine group knurl weight）/ model group knurl weight × 100%.Lung metastases inhibiting rate（%）=（Model group
Shift number-medicine group transfer number）/ model group shifts number × 100%.
5. experimental result
Chinese medicine composition of the present invention is shown in Table 3. to the inhibitory action experimental result of mice lung cancer Lewis tumours
Inhibitory action of the table 3 to mice lung cancer Lewis tumours（X ± s, n=10）
Test result indicates that, five groups of Chinese medicine compositions of the invention have obvious suppression to make to Mice Bearing Lewis lung cancer
With tumour inhibiting rate is respectively 48.3%, 60.9%, 43.9%, 34.5%, 38.0%, and can substantially reduce transfer of the tumour in lungs
Number.
6. conclusion
Chinese medicine composition of the present invention shows that it plays the role of significantly to suppress to Lewis lung cancer through mouse experiment in vivo,
And transfer number of the tumour in lungs can be substantially reduced, it therefore, it can prevent and treat the medicine of lung cancer for preparing.
Inhibitory action research of the embodiment 9 to rat liver cancer H22 tumours
1. test sample
Chinese medicine composition 1 of the present invention：Referred to as " composition 1 " is prepared by the embodiment of the present invention 1；
Chinese medicine composition 2 of the present invention：Referred to as " composition 2 " is prepared by the embodiment of the present invention 2；
Chinese medicine composition 3 of the present invention：Referred to as " composition 3 " is prepared by the embodiment of the present invention 3；
Chinese medicine composition 4 of the present invention：Referred to as " composition 4 " is prepared by the embodiment of the present invention 4；
Chinese medicine composition 5 of the present invention：Referred to as " composition 5 " is prepared by the embodiment of the present invention 5；
Control drug endoxan：Haizheng Medicine Stock Co., Ltd., Zhejiang Prov provides, code H20084627.
2. laboratory apparatus and reagent
CHD types light microscope (Japanese Olympus companies)；B204-N type electronic balances（Mettler companies of Switzerland）
Rat liver cancer ascitic type H22 tumour cells, are provided by China Concord Medical Science University's cell centre immunization experiment room.
3. experimental animal
Kunming mouse, body weight 18-22g, male, Kunming Medical University's Experimental Animal Center is provided.
4. experimental method
It is outstanding in every right side of mice armpit subcutaneous vaccination H22 liver cancer oncocyte after experiment mice adaptability is fed 1 week
Divide 7 groups at random by successful 70 mouse of modeling after liquid 0.2 mL, 7d：Model group（Equivalent distilled water）, 1 group of composition
2 groups of (20mg/kg), composition（20mg/kg), 3 groups of composition（20mg/kg), 4 groups of composition（20mg/kg), 5 groups of composition
（20mg/kg) with positive control medicine endoxan group (20mg/kg), every group 10.Each medicine group is given by prescribed dose gavage
Medicine, model group gives equivalent distilled water, 1 time a day, successive administration three weeks.In 12h after last dose, tumor mass is separated, is weighed,
Calculate tumour inhibiting rate.Tumour inhibiting rate（%）=（Model group knurl weight-medicine group knurl weight）/ model group knurl weight × 100%.
5. experimental result
Invention Chinese medicine composition is shown in Table 4. to the inhibitory action experimental result of rat liver cancer H22 tumours
Inhibitory action of the table 4 to rat liver cancer H22 tumours
（X ± s, n=10）
Test result indicates that, five groups of Chinese medicine compositions of the invention have obvious suppression to rat liver cancer H22 tumours
Effect, tumour inhibiting rate is respectively 82.1%, 74.7%, 67.5%, 52.9%, 40.5%.
6. conclusion
Chinese medicine composition of the present invention shows that it has obvious suppression to rat liver cancer H22 tumours through mouse experiment in vivo
Effect, therefore, it can prevent and treat the medicine of liver cancer for preparing.
The external inhibitory action research to the tumours of human gastric cancer SGC- 7901 of embodiment 10
1. test sample
Chinese medicine composition 1 of the present invention：Referred to as " composition 1 " is prepared by the embodiment of the present invention 1；
Chinese medicine composition 2 of the present invention：Referred to as " composition 2 " is prepared by the embodiment of the present invention 2；
Chinese medicine composition 3 of the present invention：Referred to as " composition 3 " is prepared by the embodiment of the present invention 3；
Chinese medicine composition 4 of the present invention：Referred to as " composition 4 " is prepared by the embodiment of the present invention 4；
Chinese medicine composition 5 of the present invention：Referred to as " composition 5 " is prepared by the embodiment of the present invention 5；
Control drug fluorouracil：The upper good fortune pharmaceutcal corporation, Ltd of Hisense's friendship nine provides, code H31021009
2. laboratory apparatus and reagent
SANYOMC0175 types CO 2Incubator (SANYO GS company);CLOUTE REPICS-XL fluidic cells
Instrument (Beckman-Coulter companies of the U.S.);Fluorescence microscope (Leica companies).
SGC-7901 cells, are provided by China Concord Medical Science University's cell centre immunization experiment room.
3. experimental method
It is about 1 × 10 to be configured to concentration after the SGC-7901 cells of exponential phase are digested with pancreatin7Individual/L's is thin
Cytosol, 96 orifice plates are inoculated in by 1000/hole, add 100 μ l per hole.Add and contain composition 1（Concentration 5g/L）, composition 2
（Concentration 5g/L）, composition 3（Concentration 5g/L）, composition 4（Concentration 5g/L）, composition 5（Concentration 5g/L）With reference substance medicine
Fluorouracil（Concentration 5g/L）And the fresh medium of solvent control, add 100 μ l per hole, every group sets 3 parallel holes.In 37
DEG C continue to cultivate after 72 h, abandon supernatant, the MTT nutrient solutions containing 0. 5g/L of 100 μ l Fresh are added per hole, continue training
4 h are supported, culture supernatant is abandoned, add 200 μ l DMSO per hole, is vibrated and mixed with microoscillator, with SUNRISE type enzyme marks
Instrument determines OD value (O D) under the conditions of 570 nm.Inhibiting rate of the medicine to tumour cell is calculated as follows:
Inhibiting rate（%） =（Solvent control group O D values-medicine group O D values）The % of/solvent control group O D values × 100.
4. experimental result
The external inhibitory action experimental result to human gastric cancer SGC-7901 tumours of Chinese medicine composition of the present invention is shown in Table 5.
The external inhibitory action to the tumours of human gastric cancer SGC- 7901 of table 5（X ± s, n=3）
| Medicine | Concentration（g/L） | Inhibiting rate（%） |
| Composition 1 | 5 | 52.17 |
| Composition 2 | 5 | 59.76 |
| Composition 3 | 5 | 64.58 |
| Composition 4 | 5 | 30.33 |
| Composition 5 | 5 | 21.49 |
| Fluorouracil | 5 | 55.3 |
Test result indicates that, three groups of Chinese medicine compositions of the invention have bright to the tumours of human gastric cancer SGC- 7901 in vitro
Aobvious inhibitory action, the extracorporeal inhibiting rate in the case where concentration is 5 g/L is respectively 52.17%, 59.76%, 64.58%,
30.33%, 21.49%.
6. conclusion
Chinese medicine composition of the present invention shows that it has obvious suppression to the tumours of human gastric cancer SGC- 7901 through experiment in vitro
Effect, therefore, it can prevent and treat the medicine of stomach cancer for preparing.
The tablet of embodiment 11
Chinese medicine composition 7% of the present invention
Starch 85%
Microcrystalline cellulose 8%
Above-mentioned lamp Chinese medicine composition of the present invention, starch, microcrystalline cellulose are mixed after tabletting on tablet press machine, produced.
The capsule of embodiment 12
Chinese medicine composition 30% of the present invention
Starch 61%
Magnesium stearate 9%
By the invention described above Chinese medicine composition, starch, magnesium stearate, it is packed into hard gelatin capsule, produces after mixing.
The oral liquid of embodiment 13
Chinese medicine composition 20% of the present invention
Vitamins c 1.5%
Distilled water 78.5%
By the invention described above Chinese medicine composition, vitamins c, distilled water, stir and evenly mix, be fitted into oral liquid bottle, produce.
Claims (10)
1. one kind is used for treating cancer Chinese medicine composition, it is characterised in that the bulk drug for treating cancer Chinese medicine composition
For 11 ~ 63 parts of the polygonum perfoliatum of parts by weight, 9 ~ 58 parts of Chinese lobelia, 4 ~ 52 parts of oldenlandia diffusa, 13 ~ 76 parts of clerodendron yunnanensis Hu leaf, radix dactylicapni
2 ~ 17 parts of root, 18 ~ 73 parts of madder, 4 ~ 52 parts of polygoni cuspidati,radix, 19 ~ 75 parts of rhizoma imperatae, 9 ~ 58 parts of houttuynia cordata root, 11 ~ 63 parts of rough gentian and
5 ~ 57 parts of selfheal, prepares through pre-treatment, extraction, drying.
2. it is used for treating cancer Chinese medicine composition according to claim 1, it is characterised in that the bulk drug is parts by weight
18 ~ 52 parts of polygonum perfoliatum, 35 ~ 43 parts of Chinese lobelia, 24 ~ 35 parts of oldenlandia diffusa, 31 ~ 51 parts of clerodendron yunnanensis Hu leaf, 5 ~ 15 parts of radix dactylicapni root,
43 ~ 61 parts of madder, 13 ~ 34 parts of polygoni cuspidati,radix, 29 ~ 63 parts of rhizoma imperatae, 18 ~ 49 parts of houttuynia cordata root, 19 ~ 41 parts of rough gentian and selfheal 29
~ 41 parts.
3. it is used for treating cancer Chinese medicine composition according to claim 1, it is characterised in that described to be used for treating cancer Chinese medicine
General flavone content >=2.0% in composition, total saponin content >=1.0%, rosmarinic acid contents >=0.1%, madder cellulose content >=
0.2%, and tannin, resin and oxalates must not be detected.
4. a kind of any preparation method for treating cancer Chinese medicine composition of claim 1 ~ 3, it is characterised in that including
Following steps：
A, radix dactylicapni root, polygoni cuspidati,radix crushed 10 ~ 50 mesh sieves, added acetic acid-ethanol-water mixed solvent of the weight than 2 ~ 3 times, in
4 ~ 8h is soaked at 10 ~ 30 DEG C, is transferred in percolator, the ethanol percolation of the mass concentration 30 ~ 70% with medicinal material weight than 5 ~ 7 times
24 ~ 48h is extracted, percolate is collected, filtration obtains filtrate a standby；
B, clerodendron yunnanensis Hu leaf, oldenlandia diffusa, houttuynia cordata root, selfheal, Chinese lobelia, madder, polygonum perfoliatum, rough gentian, rhizoma imperatae is cataclasm
For 1 ~ 5cm, the ethanol for adding mass concentration 50 ~ 95% is extracted, and extract solution filtration obtains filtrate b standby；
C, merging filtrate a and filtrate b, object is obtained in 40 ~ 60 DEG C of vacuum spray dryings.
5. preparation method according to claim 4, it is characterised in that second in acetic acid-ethanol-water mixed solvent described in step A
Acid, the mass ratio of second alcohol and water are 0.1 ~ 0.5:20~30:69.5~79.9.
6. preparation method according to claim 4, it is characterised in that it is to extract in three times to be extracted described in step B, for the first time
Post processing extraction solvent consumption is 8-12 times of medicinal material weight, and extraction time is 3 hours；Second of post processing extraction solvent consumption is medicinal material weight
6-8 times, 2 hours extraction times；Third time post processing extraction solvent consumption is 3-4 times of medicinal material weight, and extraction time is 1 hour.
7. preparation method according to claim 4, it is characterised in that the temperature control extracted described in step B is at 40 ~ 60 DEG C.
8. any preparation for treating cancer Chinese medicine composition of a kind of claim 1 ~ 3, it is characterised in that used described
Pharmaceutically acceptable auxiliary material is added in treating cancer Chinese medicine composition powder, tablet, capsule, granule, ball is made
Agent, oral liquid or paste.
9. any application for treating cancer Chinese medicine composition of a kind of claim 1 ~ 3, it is characterised in that described to be used for
Application of the treating cancer Chinese medicine composition in treating cancer medicine is prepared.
10. apply according to claim 9, it is characterised in that the treating cancer Chinese medicine composition that is used for is preparing treatment liver
Application in cancer, lung cancer or gastric cancer medicament.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103520625A (en) * | 2013-10-14 | 2014-01-22 | 谭钦文 | Traditional Chinese medicine for treating liver-kidney disharmony type stuffiness and fullness |
| CN103520596A (en) * | 2013-10-25 | 2014-01-22 | 扈晓楠 | Traditional Chinese medicine composition for treating bladder cancer |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103520625A (en) * | 2013-10-14 | 2014-01-22 | 谭钦文 | Traditional Chinese medicine for treating liver-kidney disharmony type stuffiness and fullness |
| CN103520596A (en) * | 2013-10-25 | 2014-01-22 | 扈晓楠 | Traditional Chinese medicine composition for treating bladder cancer |
Non-Patent Citations (3)
| Title |
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| 原发性肝癌的中医药防治研究;王羲明;《中医药研究》;19910131(第1期);40-43页 * |
| 周仲瑛教授从癌毒辨治肿瘤经验;陈四清;《新中医》;20040229;第36卷(第2期);7-9页 * |
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